CN112646820B - Gene and method for changing flowering period of corn - Google Patents

Abstract

The present invention is in the field of molecular genetics. In particular to a gene and a method for changing the flowering period of corn. The invention provides a sequence of a gene for controlling the flowering phase of corn and discloses a method for changing the flowering phase by mutating the gene by using a genetic engineering means. The invention also provides a maize mutant gene sequence with an altered flowering phase, which can be used for improving maize flowering phase traits.

Description

Gene and method for changing flowering period of corn

Technical Field

The present invention is in the field of molecular genetics. In particular to a gene for controlling the maize florescence and application thereof in changing maize florescence characters. The invention provides a sequence of a gene for controlling the flowering phase of corn and discloses a method for shortening the flowering phase by mutating the gene by using a genetic engineering means. The invention also provides a maize mutant gene sequence with shortened flowering phase, and the mutant gene sequence can be used for improving maize flowering phase characters.

Background

In higher plants, flowering represents a transition from vegetative to reproductive growth, which plays an important role throughout the growth and development stages of the plant. The biological character of when to bloom is subjected to the dual functions of the genetic factors of the plant body and the external environmental factors. Under the influence of the dual action, a series of flowering induction processes are common in higher plants, namely, the leaves of the plants generate flowering substances (or florigen) at proper time by sensing external growth conditions (light, temperature, humidity and the like), and the flowering substances are conveyed to the stem tip growing point from the leaves through the conduction tissues to stimulate the apical meristem to flower.

The flowering phase is an important character in the crop evolution and adaptation process, the understanding of the genetic basis of the character of the crop flowering phase and the cloning of candidate genes can improve the environmental adaptability and plasticity of the crop, which has important significance for cultivating good crop varieties adapting to different ecological regions, and simultaneously, the genetic improvement process of important production characters such as yield and the like closely related to the flowering phase can be promoted.

CCT (CO, COL and TOC1) family genes are widely involved in the regulation and control process of plant flowering phase and play an important role in the growth and development of plants. The maize B73 reference genome has 53 genes with CCT structure, and the applicant previously used a related population composed of 368 maize inbred lines to locate 34 CCT genes related to maize flowering (Jinminghai construction of maize pan transcriptome and functional analysis of maize flowering inhibitor ZmCOL3 [ D ]. Hubei: university of China agriculture, 2018; Jin M, Liu X, Jia W, et al. ZmCOL3, a CCT gene expression in mail flowering by maize flowering with the cyclic approach and activation expression of ZmCCT [ J ]. J Integr Plant biol.,2018,60(6):465 and 480.), most of which have no specific functional identification research results, so that the specific genes can really control maize traits incompletely and completely. If a more precise change in maize flowering phase is desired, it is necessary to specify which genes will control maize flowering phase traits and to what extent these genes have been genetically engineered to produce the changes in flowering phase traits.

In order to solve the problems, the invention utilizes a gene editing technology to mutate 15 genes in the 34 genes, obtains the genes and mutant genes which can influence the traits of the maize in the flowering phase through phenotype identification, and provides a method for artificially changing the maize flowering phase. The genes and the method can be used for artificially regulating the flowering phase of the corn and cultivating new corn materials which are suitable for different ecological environments.

Disclosure of Invention

One of the purposes of the invention is to provide a gene sequence for controlling the flowering character of corn.

The invention also aims to disclose a method for changing the flowering phase of the corn.

The invention also aims to provide a mutant gene for changing the flowering phase trait of the corn.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides an application of a corn gene in controlling the traits of corn in the flowering period, which is characterized in that: the gene sequence is shown in SEQ ID NO. 1. The SEQ ID NO.1 sequence is the genome sequence of GRMZM2G005732 gene in a maize inbred line B73.

The invention also provides a method for advancing the flowering phase of corn, which is characterized by comprising the following steps: inhibiting the expression and/or activity of the protein coded by the gene in the corn, and selecting the plant with early flowering phase of the corn.

In some embodiments, the method of inhibiting protein expression and/or activity comprises any one of gene editing, RNA interference, T-DNA insertion, physical or chemical mutagenesis.

In some embodiments, the above gene editing employs the CRISPR/Cas9 method.

In some embodiments, the DNA sequence of the genomic target region in maize of the CRISPR/Cas9 method described above is shown as SEQ ID No.2 or SEQ ID No. 3.

The invention also provides a kit for advancing the flowering phase of corn, which is characterized in that: including any of the following:

(1) the sequence of the sgRNA molecule is shown in SEQ ID NO.4 or SEQ ID NO. 5;

(2) a DNA molecule encoding the sgRNA;

(3) a vector expressing the sgRNA.

The invention also provides a mutant gene for advancing the flowering phase of corn, which is characterized in that: the sequence of the mutant gene is shown as SEQ ID NO.6 or SEQ ID NO. 7.

A plurality of different editing types can be obtained by gene editing mutation target genes, and the plants corresponding to the different editing types do not perform completely the same. Through screening and identification, the mutant gene shown in SEQ ID NO.6 or SEQ ID NO.7 is determined to lead the maize to be moderately early in flowering phase. The mutant gene can be introduced into corn materials with different genetic backgrounds in a sexual hybridization mode, so that a new early-flowering corn variety is created.

The present invention also provides a primer set for detecting the above mutant gene, characterized in that: the primer pair is a sequence shown in SEQ ID NO.8 and SEQ ID NO.9 or a complementary sequence thereof.

The invention also provides application of the primer pair in detecting the mutant gene. And carrying out PCR amplification on the genome DNA of the sample to be detected by using the primer pair, sequencing and analyzing the sequence of the amplification product, wherein the sample to be detected contains the mutant gene if the sequence of the sequenced amplification product is consistent with a partial sequence of the sequence shown by SEQ ID NO.6 or SEQ ID NO. 7.

The invention also provides a method for delaying the flowering phase of corn, which is characterized by comprising the following steps: improving the expression and/or activity of the sequence protein shown as SEQ ID NO.11 in the corn, and selecting the plants with delayed flowering phase of the corn.

The invention has the following advantages and beneficial effects: the invention utilizes the related population to locate 34 CCT genes related to the maize flowering phase trait, however, the specific genes in the candidate genes are not known to really control the maize flowering trait and influence degree on the flowering phase trait. The invention utilizes gene editing technology to mutate 15 of the 34 genes, and determines that the GRMZM2G005732 can really affect the maize flowering phase character through phenotype identification. The method for editing the CRISPR/Cas9 gene can be used for advancing the flowering period of the corn, and the edited mutant gene can be used for creating a new early-flowering corn variety. And the flowering period of the corn can be delayed by using a strong promoter to excessively express the GRMZM2G005732 gene coding region, and the obtained over-expression plant can be used for creating a new late-flowering corn variety. The invention provides a new gene and a new method for manually regulating the flowering period of corn to culture a new corn material suitable for different ecological environments.

Drawings

FIG. 1 Gene editing vector map. The English and abbreviated meanings of each element are listed as follows:

RB T-DNA repeat T-DNA right border repeat

M13 fwd M13 primer sequence (Forward)

p000204_1F target gRNA sequence

Ubi promoter ubiquitin promoter

3 × FLAG tag sequence

SV40NLS Simian Virus 40 Nuclear localization Signal

Cas9 Cas9 gene sequence

Nucleoplasm in NLS nuclear localization Signal

NOS terminator of nopaline synthase

lac promoter lactose promoter

M13 rev M13 primer sequence (reverse)

lac operator lactose operon

CAP biding site CAP binding site

CaMV35S promoter (enhanced) enhanced cauliflower mosaic virus 35S promoter

BlpR-encoded Bar protein confers glufosinate tolerance in plants

CaMV35S polyA single cauliflower mosaic virus 35S polyadenylation sequence

LB T-DNA repeat T-DNA left border repeat

Kan R kanamycin resistance sequence

Ori initiation region sequence

Bom framework region sequence

pVS1 RepA pVS1 replicon

pVS1 StaA pVS1 transcriptional initiation region

FIG. 2 is a diagram of a gene overexpression vector. The English and abbreviated meanings of each element are listed as follows:

t Border (R) T-DNA right BORDER sequence

pVS1 Sta pVS1 replication initiation site

pVS1-REP pVS1 replicon

Bom site of pBR322 bom site vector

pBR322 ori pBR322 vector replication initiation site

aadA streptomycin resistance sequences

T Border (L) T-DNA left BORDER sequence

tVsp Soy storage protein 3UTR terminator

Screening marker gene for tolerance to barfosinate-ammonium

PeCaMV35S cauliflower mosaic virus 35S promoter

pZmUBI pZmUBI promoter

TEV tobacco etch virus enhancer

GRMZM2G005732-T01 GRMZM2G005732-T01 transcript sequences

tNOS nopaline synthase terminator

Detailed Description

The following definitions and methods are provided to better define the present application and to guide those of ordinary skill in the art in the practice of the present application. Unless otherwise indicated, terms are to be understood in accordance with their ordinary usage by those of ordinary skill in the relevant art. All patent documents, academic papers, industry standards and other publications, etc., cited herein are incorporated by reference in their entirety.

As used herein, "maize" is any maize plant and includes all plant varieties that can be bred with maize, including whole plants, plant cells, plant organs, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, intact plant cells in plants or plant parts, such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruits, stems, roots, root tips, anthers, and the like. Unless otherwise indicated, nucleic acids are written from left to right in the 5 'to 3' direction; amino acid sequences are written from left to right in the amino to carboxy direction. Amino acids may be referred to herein by their commonly known three letter symbols or by the one letter symbols recommended by the IUPAC-IUB Biochemical nomenclature Commission. Similarly, nucleotides may be represented by commonly accepted single-letter codes. Numerical ranges include the numbers defining the range. As used herein, "nucleic acid" includes reference to deoxyribonucleotide or ribonucleotide polymers in either single-or double-stranded form, and unless otherwise limited, includes known analogs (e.g., peptide nucleic acids) having the basic properties of natural nucleotides that hybridize to single-stranded nucleic acids in a manner similar to naturally occurring nucleotides. As used herein, the term "encode" or "encoded" when used in the context of a particular nucleic acid means that the nucleic acid contains the necessary information to direct translation of the nucleotide sequence into a particular protein. The information encoding the protein is represented using a codon. As used herein, "full-length sequence" in reference to a particular polynucleotide or protein encoded thereby refers to the entire nucleic acid sequence or the entire amino acid sequence having a native (non-synthetic) endogenous sequence. The full-length polynucleotide encodes the full-length, catalytically active form of the particular protein. The terms "polypeptide" and "protein" are used interchangeably herein to refer to a polymer of amino acid residues. The term is used for amino acid polymers in which one or more amino acid residues are artificial chemical analogues of the corresponding naturally occurring amino acids. The term is also used for naturally occurring amino acid polymers. The terms "residue" or "amino acid" are used interchangeably herein to refer to an amino acid that is incorporated into a protein, polypeptide, or peptide (collectively, "protein"). The amino acid can be a naturally occurring amino acid, and unless otherwise limited, can include known analogs of natural amino acids that can function in a similar manner as naturally occurring amino acids.

As used herein, the terms "isolated" and "purified" may be used interchangeably to refer to a nucleic acid or polypeptide, or biologically active portion thereof, that is substantially or essentially free of components that normally accompany or react with the nucleic acid or polypeptide as found in its naturally occurring environment. Thus, an isolated or purified nucleic acid or polypeptide produced by recombinant techniques is substantially free of other cellular material or culture medium, or is substantially free of chemical precursors or other chemicals when chemically synthesized. An "isolated" nucleic acid is typically free of sequences (such as sequences encoding proteins) that naturally flank the nucleic acid (i.e., sequences located at the 5 'and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, an isolated nucleic acid may comprise less than about 0.5kb of nucleotide sequences that naturally flank the nucleic acid in the genomic DNA of the cell from which the nucleic acid is derived.

In this application, the words "comprise", "comprising" or variations thereof are to be understood as embracing elements, numbers or steps in addition to those described. By "subject plant" or "subject plant cell" is meant a plant or plant cell in which the genetic modification has been effected, or a progeny cell of the plant or cell so modified, which progeny cell comprises the modification. The "control" or "control plant cell" provides a reference point for measuring the phenotypic change of the test plant or plant cell. The control plant or plant cell may include, for example: (a) a wild-type plant or cell, i.e., a plant or cell having the same genotype as the starting material for the genetic alteration that produced the test plant or cell; (b) plants or plant cells having the same genotype as the starting material but which have been transformed with an empty construct (i.e., a construct that has no known effect on the trait of interest, such as a construct comprising a target gene); (c) a plant or plant cell that is a non-transformed isolate of a subject plant or plant cell; (d) a plant or plant cell that is genetically identical to the subject plant or plant cell but that has not been exposed to conditions or stimuli that induce expression of the gene of interest; or (e) the subject plant or plant cell itself, under conditions in which the gene of interest is not expressed.

Those skilled in the art will readily recognize that advances in the field of molecular biology, such as site-specific and random mutagenesis, polymerase chain reaction methods, and protein engineering techniques, provide a wide range of suitable tools and procedures for engineering or engineering amino acid sequences and potential gene sequences of proteins of agricultural interest.

In some embodiments, changes may be made to the nucleotide sequences of the present application to make conservative amino acid substitutions. The principles and examples of conservative amino acid substitutions are further described below. In certain embodiments, substitutions that do not alter the amino acid sequence of the nucleotide sequences of the present application can be made in accordance with the codon preferences disclosed for monocots, e.g., codons encoding the same amino acid sequence can be substituted with monocot preferred codons without altering the amino acid sequence encoded by the nucleotide sequence. In some embodiments, a portion of the nucleotide sequence in this application is replaced with a different codon that encodes the same amino acid sequence, such that the nucleotide sequence is not altered while the amino acid sequence encoded thereby is not altered. Conservative variants include those sequences that, due to the degeneracy of the genetic code, encode the amino acid sequence of one of the proteins of the embodiments. In some embodiments, a partial nucleotide sequence herein is replaced according to monocot preferred codons. One skilled in the art will recognize that amino acid additions and/or substitutions are generally based on the relative similarity of the amino acid side-chain substituents, e.g., hydrophobicity, charge, size, etc., of the substituents. Exemplary amino acid substituent groups having various of the foregoing properties are known to those skilled in the art and include arginine and lysine; glutamic acid and aspartic acid; serine and threonine; glutamine and asparagine; and valine, leucine and isoleucine. Guidance regarding suitable amino acid substitutions that do not affect the biological activity of the Protein of interest can be found in the model of the Atlas of Protein sequences and structures (Protein Sequence and Structure Atlas) (Natl. biomed. Res. Foundation, Washington, D.C.) (incorporated herein by reference). Conservative substitutions such as exchanging one amino acid for another with similar properties may be made. Identification of sequence identity includes hybridization techniques. For example, all or part of a known nucleotide sequence is used as a probe for selective hybridization to other corresponding nucleotide sequences present in a population of cloned genomic DNA fragments or cDNA fragments (i.e., a genomic library or cDNA library) from a selected organism.

In some embodiments, fragments of the nucleotide sequences and the amino acid sequences encoded thereby are also included. As used herein, the term "fragment" refers to a portion of the nucleotide sequence of a polynucleotide or a portion of the amino acid sequence of a polypeptide of an embodiment. Fragments of the nucleotide sequences may encode protein fragments that retain the biological activity of the native or corresponding full-length protein, and thus have protein activity. Mutant proteins include biologically active fragments of the native protein that comprise contiguous amino acid residues that retain the biological activity of the native protein. Some embodiments also include a transformed plant cell or transgenic plant comprising the nucleotide sequence of at least one embodiment. In some embodiments, plants are transformed with an expression vector comprising at least one embodiment of the nucleotide sequence and operably linked thereto a promoter that drives expression in plant cells. Transformed plant cells and transgenic plants refer to plant cells or plants that comprise a heterologous polynucleotide within their genome. Generally, the heterologous polynucleotide is stably integrated within the genome of the transformed plant cell or transgenic plant such that the polynucleotide is transmitted to progeny. The heterologous polynucleotide may be integrated into the genome alone or as part of an expression vector. In some embodiments, the plants to which the present application relates include plant cells, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, plant clumps, and plant cells, which are whole plants or parts of plants, such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruits, nuts, ears, cobs, husks, stalks, roots, root tips, anthers, and the like. The present application also includes plant cells, protoplasts, tissues, calli, embryos, and flowers, stems, fruits, leaves, and roots derived from the transgenic plants of the present application or progeny thereof, and thus comprising at least in part the nucleotide sequences of the present application.

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Modifications or substitutions to methods, steps or conditions of the present invention may be made without departing from the spirit and substance of the invention and are intended to be included within the scope of the present application. Unless otherwise indicated, the examples follow conventional experimental conditions, such as the Molecular cloning laboratory Manual of Sambrook et al (Sambrook J & Russell D W, Molecular cloning: a laboratory Manual,2001), or the conditions as recommended by the manufacturer's instructions. Unless otherwise specified, the chemical reagents used in the examples are all conventional commercially available reagents, and the technical means used in the examples are conventional means well known to those skilled in the art.

Examples

Example 1 identification of maize flowering phase changes following Gene editing knockout of candidate genes

The invention utilizes CRISPR-Cas9 gene editing technology to carry out site-directed editing on 15 genes in 34 genes related to the flowering phase of corn shown by a correlation analysis result.

The implementation mode comprises the construction of a gene editing vector, the genetic transformation of corn and the functional verification of editing effect. The method comprises the following specific steps:

1. construction of Gene editing vector

The gene editing vector of the invention is G08943-CPB-ZmUbi-hspCas9, and the vector diagram is shown in figure 1. The basic vector of the vector is CPB-ZmUbi-hspCas 9. The invention obtains double target U6-sgRNA through overlapPCR and clones the double target U6-sgRNA into a basic vector through homologous recombination, and the specific construction process is as follows:

(1) cloning of the U6 promoter. The U6 promoter was cloned from B73.

(2) Design of target gRNA. The recipient material B73 reference genomic sequence was imported into http:// cbi.hzau.edu.cn/criprpr/for target design.

(3) U6-sgRNA was obtained by Overlap PCR. The primer pair U6F1/U6R is used for amplifying the U6 promoter of the first target, and the product length is 515 bp; the primer pair gR-1F (3F)/gRR1 is used for amplifying the sgRNA of the first target, and the product length is 127 bp; primer pair U6F1/gRR1 was used to perform the Overlap PCR step 2 amplification (U6-sgRNA) with a product length of 634 bp. U6 and sgRNA are respectively amplified in the 1 st step of the Overlap PCR, and PCR products are respectively diluted by 50 times and then mixed to be used as a template for carrying out the 2 nd step amplification of the Overlap PCR. And (5) electrophoresis gel cutting recovery and sequencing of the amplification product to confirm the sequence. The Overlap PCR system and conditions were as follows: the 15. mu.L reaction in step 1 of the Overlap PCR was as follows, template DNA (U6 or sgRNA,. gtoreq.30 ng/. mu.L): 0.5. mu.L, Primer F/R: 1.2 μ L each, sterile ddH 2O: 3.7 μ L,2 × phanta max Buffer: 7.5 μ L, dNTP mix: 0.6. mu.L, Phanta enzyme (product No.: P505-d1/d2/d 3): 0.3. mu.L. The reaction system in step 2 of the Overlap PCR was a 30. mu.L system. U6 pipetted 1. mu.L, diluted with 49. mu.L ddH 2O; sgRNA aspirated at 1. mu.L, diluted with 49. mu.L of ddH2O, aspirated at 10. mu.L each, and mixed well. The method comprises the following specific steps: mixed template DNA (U6+ sgRNA): 1.5 μ L, Primer F/R: 2.4 μ L each, sterile ddH 2O: 6.9 μ L,2 × phanta max Buffer: 15 μ L, dNTP mix: 1.2. mu.L, Phanta enzyme: 0.6. mu.L. The Overlap PCR program was as follows: (1)94 ℃ for 5 minutes, (2)94 ℃ for 30 seconds, (3)62 ℃ for 35 seconds, (4)72 ℃ for 30 seconds, and the (5) th step is a cycle of 32 times from the (2) step to the (4) step, (6)72 ℃ for 10 minutes, and (7)25 ℃ for 5 minutes. The primer sequences required for vector construction are shown in Table 1.

TABLE 1 primer sequences required for vector construction

(4) The construction into a backbone vector is carried out by recombinant cloning. The CPB-Ubi-hspcas9 vector was digested with HindIII and recovered. Both U6-gRNA and the vector were ligated by homologous recombination. Before reaction liquid preparation, the concentration of each Overlap product is ensured to be close to be consistent, and a 20 mu L homologous recombination system comprises the following steps: cas Hind III: 3 μ L, T-1F Overlap: 1 μ L, sterile ddH 2O: 10 μ L, 5 × CE MultiS buffer: 4 μ L, Exnase MultiS (product No.: C113-01/02): 2 μ L.

2. Genetic transformation of maize

The vector is transferred into agrobacterium EHA105 by an electric shock method, and PCR is carried out for identification. Taking a freshly peeled young embryo of a maize inbred line KN5585 (an inbred line bred by Mimi Biotechnology (Jiangsu) Co., Ltd.) of about 1mm as a material, putting the peeled maize embryo into a 2mL plastic centrifuge tube containing 1.8mL of suspension, and treating about 150 immature young embryos within 30 min; the suspension was aspirated, the remaining corn embryos placed in a tube and then 1.0mL of Agrobacterium suspension was added and left for 5 min. The young embryos in the centrifuge tube are suspended and poured onto a co-culture medium, and the surplus agrobacterium liquid on the surface is sucked by a liquid transfer device and is cultured for 3 days in the dark at the temperature of 23 ℃. After co-cultivation, the young embryos were transferred to a resting medium, cultured in the dark at 28 ℃ for 6 days, placed on a screening medium containing 5mg/L of Bialaphos, and screened for 2 weeks, and then transferred to a screening medium containing 8mg/L of Bialaphos for 2 weeks. The resistant calli were transferred to differentiation medium 1 and cultured at 25 ℃ under 5000lx light for 1 week. Transferring the callus to a differentiation culture medium 2, and culturing for 2 weeks by illumination; transferring the differentiated plantlets to a rooting culture medium, and culturing at 25 ℃ and 5000lx by illumination until the plantlets are rooted; transferring the plantlets into small pots for growth, transplanting the plantlets into a greenhouse after a certain growth stage, and harvesting progeny seeds after 3-4 months.

3. Trait identification of gene-edited plants

The flowering phase traits of the obtained gene editing material are identified, and some of the genes can really cause the significant change of the flowering phase of the corn after being edited, while the obvious flowering phase change cannot be observed after some genes are edited, and the specific change mode is shown in table 2.

TABLE 2 flowering-time Change after Gene editing

Example 2 in-depth analysis of maize flowering-stage traits and identification of mutant genes

And carrying out more deep character identification on the gene editing material with the flowering character change, and analyzing specific editing sites. After editing of this gene GRMZM2G005732, maize flowering time was earlier for the T0 generation material than for the receptor control KN 5585. The flowering time of the T1 generation material of this transformant was more deeply characterized and analyzed for specific editing sites.

Two target sites designed during editing of the GRMZM2G005732 gene are shown as SEQ ID NO.2 and SEQ ID NO.3 respectively. gRNA sequences expressed by the vector containing the two targets are respectively shown as SEQ ID NO.4 and SEQ ID NO. 5.

Extracting DNA in seedling stage to detect gene editing condition. Designing a primer, wherein the sequence of the primer is shown as SEQ ID NO.8 and SEQ ID NO. 9. Amplifying a target editing section, wherein an amplification system is as follows: DNA: 3 μ L, 1 μ L each of the bidirectional primers, 2 × TaqMix: 7.5 μ L, ddH₂O: 2.5. mu.L, total volume 10. mu.L. The PCR reaction conditions were as follows: (1)94 ℃ for 5 minutes, (2)94 ℃ for 40 seconds, (3)57 ℃ for 30 seconds, (4)72 ℃ for 60 seconds, (5) cycle 35 times from (2) step (4), (6)72 ℃ for 7 minutes, and (7) storage at 4 ℃. The PCR product was submitted to Sanger sequencing by Wuhan Strongziaceae Biotech Ltd. And comparing the PCR amplification sequencing results of the transformant and the receptor KN5585, wherein the material subjected to base substitution, insertion or deletion is a positive editing material, and the material is a negative material otherwise.

After sequence comparison, 2 different editing types of materials are found, and the target points of the two materials are mutated. Wherein the A1 material was deleted 186bp between two targets and 214bp between two targets of A2 (Table 3). Thus, after editing, the gene sequences of both A1 and A2 changed from SEQ ID NO.1 to SEQ ID NO.6 and SEQ ID NO. 7.

TABLE 3 Gene editing maize Material flowering time trait data

"in ═ represents a deletion sequence, boxes represent PAM sequences, and underlines represent editing targets.

The flowering stage traits (including the emasculation stage, the pollen scattering stage and the silking stage) of the materials A1 and A2 were experimentally investigated in Guilin province in summer 2018, and the results are shown in Table 4. The flowering phase of the edited materials of A1 and A2 is obviously earlier than that of the unedited control material, and the fact that the gene controls the flowering phase trait and the flowering phase is advanced after gene editing is proved.

TABLE 4 Gene editing maize Material flowering time trait data

Florescence data are expressed as mean ± standard deviation, in units: and (5) day. "CK" represents unedited control material. "x" indicates a very significant difference compared to the control (P < 0.01).

Therefore, the mutant gene shown in SEQ ID NO.6 or SEQ ID NO.7 can lead the maize to be moderately early in flowering phase. The mutant gene can be introduced into corn materials with different genetic backgrounds in a sexual hybridization mode, so that a new early-flowering corn variety is created.

In the process of introduction, a primer pair with sequences shown in SEQ ID NO.8 and SEQ ID NO.9 is utilized to detect whether the maize genome contains the mutant gene, the primer pair is used for carrying out PCR amplification on the genome DNA of a sample to be detected, and if the size of an amplification product is 321bp, the mutation gene shown in SEQ ID NO.6 is contained; if the size of the amplified product is 293bp, the mutant gene comprises a mutant gene shown as SEQ ID NO. 7; if the amplification product size is 507bp, the gene type is unedited.

Example 3 delaying maize flowering by means of overexpression

The invention further utilizes an overexpression technology to carry out manual operation on the expression of the GRMZM2G005732 gene, and investigates the phenotype of the corn plant after the expression is changed. The specific process is as follows:

1. and constructing a overexpression vector.

A plurality of transcripts exist in the GRMZM2G005732 gene, and the invention constructs a GRMZM2G005732 gene T01 transcript overexpression vector pTF101-pZmUBI-GRMZM2G005732-T01 by taking pTF101-pZmUBI as a framework vector. The method comprises the following specific steps: taking leaves of 4 leaf stage of corn, extracting mRNA, carrying out reverse transcription to obtain cDNA, and cloning CDS sequence (SEQ ID NO.10) of GRMZM2G005732 gene T01 transcript by PCR. The amino acid sequence encoded by the CDS sequence of the T01 transcript is shown in SEQ ID NO. 11.

The pTF101-pZmUBI framework vector is subjected to double enzyme digestion by BamHI and SpeI, and is connected with a cDNA sequence of a T01 transcript of GRMZM2G005732 gene to obtain a super-expression vector which is pTF101-pZmUBI-GRMZM2G 005732-T01. The vector takes pZmUBI as a promoter to drive the overexpression of a T01 transcript of GRMZM2G005732 gene. The figure of the overexpression vector is shown in FIG. 2. The expression cassette sequence of pTF101-pZmUBI-GRMZM2G005732-T01 is shown in SEQ ID NO. 12.

2. Corn genetic transformation and transgenic material creation.

Refer to the conversion procedure of example 2. The transgenic corn of T0 generation obtained by research is backcrossed and transformed by adopting HiII (from A188 multiplied by B73) as receptor material and adopting a commercial corn inbred line B73 improved line as a recurrent parent. The obtained BC2 generation material is used for carrying out field phenotype identification.

3. Phenotypic identification of overexpressing plants

The flowering phase characters (including the emasculation phase, the pollen scattering phase and the silking phase) of the over-expressed material were experimentally investigated in Jilin province in summer 2020, and the results are shown in Table 5. The positive over-expression material of the OE-44 transgenic plant has a significantly delayed flowering phase compared with the control material of the negative segregation; the heading period and the pollen scattering period of the positive over-expression materials of the OE-31 transgenic plants and the OE-35 transgenic plants are obviously delayed compared with those of negative control materials; the flowering time of the over-expressed material of the OE-29 transgenic plants was not significantly different compared to the control, but still showed a tendency to delay flowering. In the transgenic plants, the expression of the exogenous gene is influenced by the difference of the insertion positions of the exogenous sequences into the genome, so that the property expression of different transgenic materials has difference, and the materials with excellent properties can be obtained by property identification and screening. The OE-44 transgenic material has the characteristic of obvious late flowering, and the transformation event can be introduced into corn materials with different genetic backgrounds in a sexual hybridization mode, so that a new late-flowering corn variety is created.

TABLE 5 flowering time trait data for over-expressed corn material

Florescence data are expressed as mean ± standard deviation, in units: and (5) day. "+" indicates positive over-expressed material. "-" indicates negative control material. "+" indicates a very significant difference compared to the control (P <0.01), "+" indicates a significant difference compared to the control (P < 0.05).

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Sequence listing

<110> university of agriculture in Huazhong, college of agricultural sciences of Jilin province, Miami Biotechnology (Jiangsu) Co., Ltd

<120> genes and methods for altering flowering phase of maize

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gcaggtcgtc agtgccctgc ttcgttgctg catgtaccaa ggtttgtttt atcccaattc 360

atccttcagt cgtttttctg acaggctatg atttcgcata ttcatagcat cgatcataac 420

ttggagctgt ttaacatcta taaataccaa ttcgcagcat atagatatgg aacaccgaaa 480

tgataaacat cggtctcagt ttcagtgttt cataaacatt tgcaaagtat catggataat 540

tgatatattg aaacagtaag gcaactgacc aaatcaactt acaatttcaa ctcgcggttg 600

cccgttagta atctaccaca gctggtacat agtagatggc ctcttgcagc cgaggaagga 660

gcgctaggcg tgacagtggt gtacggactg gattgctccc gatcggtgga gcagggagag 720

cggtagcata ggacatcaca agtgatgacg atgccaacgt ggctgtgcaa caagagccac 780

acttggtagt gtttagggtt ccatgatcag ggtgaccaca tcgggtcggc tatgcctatg 840

aaaatggtgc gtgagggagc aacacctatg gaggccacgc tagaggacct gcaggaagga 900

tacatagtgt tgagtcaatg ctgcgacata gattagcctg ttcatgcaag aatgcaaggt 960

ccgttaggag atggtgtagc aaaaggaaaa atggactcat tgaacacgac atgtcaaaag 1020

atgatgatcc tattggattg caagttgagg caccgatatc ctttaggctc cgtttggatg 1080

tcgatattgg agggcaagga attgaattgg gttcaatacc aaatcaacca tgatattgaa 1140

ccaattctat tgtttggatg tcactgaatt ggagtttgga attgtgcggt ctaatccacg 1200

caataccgag gggtgatgct ctgtattgga aagttgagtt tctagttata gtccaattcc 1260

agggaattgg gtctctgatt ccaaacctca attctatgtg caaccaaaca acaaaatttt 1320

gaaaagctga ttccaattct caattttgtg ttccaatatc tacatccaaa cagggtatta 1380

ggccctcatt ggaatgtggg aatttttgct gtttcctgcg tttttcctgt gaaaatgaac 1440

tgattcttgt aaaattcttg tatgattcct ctgaaattcc tgcattccaa agggggcctt 1500

atagtgagga gagtacccaa gaaagacaca taaaatgtac tgagccgcaa gtttatggct 1560

agtagtggca gaaaggttgg ggtaacaagt acaaccaaaa acacgaagat cattcatcgg 1620

tggggagcca gggaaggtga tgcgtggggt ttagtaattt agtgcctgta ggaaggatgt 1680

taagaaggtt ggtgactgta tgcagggttt cagcccagta atgaggagca aataatgttg 1740

ttgatagatc ggatgatgcg tttagccttg ccattttgag tcgaagtgta ggggcatgac 1800

atgcttgagt tgggcaccat gtgtaaggaa gaaggcgcgg gcgctagatt ctatcaagct 1860

cgtggctatt gtcgcacact cgcactagat ggccttaacg gaggcactga actaagtgga 1920

gacataagaa aaaaaataga gtagggaacg tgtccgactt gagtagcagt ggaaaaatcc 1980

aaaaaaaata tgaacaacca tcacaaataa ccaaatagta tttgtaactg gagacaatgg 2040

caacatgaag tccacaagtc acaatgtata aggttgaaat tattcttaac atgagatgtt 2100

ggcacattga aagaaagatg aacacgacga ccgagctaac acgcatgaca taaagtgctc 2160

atatctttat tacaagactg gatggcagaa taatgaacaa gtttggttag tgcctcgtgg 2220

ctgggatgta tgagatgtct ccagagcggg gatgaggtgc ttttgctttt ggtagcttgg 2280

taagaagagt acggattgca taaatgatgc tttagcggca tggctatgct agcgctcaga 2340

cgtctaatgg gtgctaacgt ttgaacactg gtggggccca ccgttgcaac atatacaaca 2400

tcactttgaa ctatcgcgac atccagttga actgttgcaa catttgaaac atgcatttga 2460

acacataaaa caactataac acacttgcac attcaaaaaa catttgttgc aacatctcga 2520

aaatcccatg caacacagaa acaatgaaca caagtacaca acattataaa tcagaaacca 2580

caacattgtt ggtcatatgg gctaggatgt acaacaacaa ggggattaat ttgaggccca 2640

aattgcaact ctagccattg gcccatcacc tttgagccag ttggagggag gagggaatag 2700

gtccccacct ttggcaccgg tcctatgact cctcgtcctc ctatttccct aggtcatatc 2760

gggctcctcg cgccaaggga tcgaagtccc atgagccccc tctgtgggct gaagtaggga 2820

ggtagtatga gcagccgagg atgatggcgt gcagacatcg ccaccacgga ggccatcgta 2880

tgctccgttg gcctcaactt tgacatactt ggtgaagagt gtggataaga atgagtgagt 2940

ggggaggaga gagcgttgtt gggagtgtgc caaacatgtg ttagatattt ctagcgatgt 3000

ctggatactt ttaaagtagc atttccattg ttaataggcc acctatcggc aacactcatt 3060

gcttggcgaa ggaaactcac attctaatat gtctatattg ttttgaacga actttttagc 3120

attttttttg cattgggtat tttttaaact taaaaatact gaactttgaa atcttggctc 3180

cactactgtg ggagaggtgc cactgtagga gacatgggtg cttaacattg gaggatagta 3240

gagcgatcga cgtgagttgc tagtgctcaa tgtctggagg ggacgagaag atactctcgt 3300

ttttttctcg aacacgtagg agagctgcgt atcattgcat taaaagatag aagaacaata 3360

caggggtcaa aaggtcctac acggaaaaga aagaaagaaa aaacacgaca ctaaaacatc 3420

tactccagag aagcaacaca tgcctcaacc taggtcatga ccataaacta agagcaagga 3480

caaaccctta gcaccagcaa gacaccacaa attacattca tcctcagcta aagacaaggc 3540

cctagcgaca ctaggcagaa cgccttcaaa aacagacacg ttgcagtgtc gccatataca 3600

ccaagctcct aaaatcacta gagttgaagc ctttttggat gcctccatta aggcgagtaa 3660

ttgaagtact gcaccaagcc tcaaaattgc tttcctcgat gcccagggac aacgcagcca 3720

gtcccctctt ctggaagatg ttgaaccaaa attgtctagc gaaaacataa gacaccaaca 3780

aatgatgaat tgtttcgtcc ttttggtcac ataaggggca aaagaccgaa tgtgaaaggc 3840

ctcttttagc caacctatca gctgtccaac accggttgtg catcacaagc caaagaaaaa 3900

acaacatttg gccggcaccc aagattgtca aatgcgctca aaaggaccaa aagaagtaga 3960

gccactgaac aacaaatcat aagccgactt agaggagtac tttccacaat tggaaaggct 4020

ccaaaaatgc ttaccatgaa tcgcatgctg cagcacataa tcatagaaaa cttcccaaag 4080

ggtcaaaaag tccaggatga cctcctagaa aacaaccccc ctgaaacaat tagtccaacg 4140

tgaattttcc aaagcctttt tgggacaagg gcgaccacac gaggggtata gtccaccagt 4200

ctcggagcca acaaccatct atcttgccaa aaagagtagt tttgccatct cccatattaa 4260

aggccataac aaataagaat atggactgca ccatagagtg ggactgcatt ggaagagcag 4320

cccaaggttt taaagagtca gttttttttg caaccacaac catctgccat gcaaggccca 4380

atttaaatac tgaagattgg aaataccaag accctcaaga tctttaggtc gagtggcagt 4440

gggccatgcc actaaacagt gacccccgtt ggcatccttg cgacctgtcc aaagaaaact 4500

acgacgaatc ttgtcaaagg ccttaatcgc ccactttgga agctcaagag ccatagcacg 4560

gtaaataatg gtggtagtga ggacagactg aactaggatg gccctgccag cctagtcatc 4620

aatgatttat tctagaggtt gtcgagaaca actatagcag gattgtccag gtggtggtgc 4680

caaatggagg aagagacgat aaggctagct tgagctttat tctaggggtt gtcgagaacg 4740

actatagtaa tatgatcaag gtggtggtgc taaacgaagg atgagatgat aaggttagct 4800

tgagttgcag gggtggtgac tagggggaag gtgttaagat tattgccatt attcaaacaa 4860

agaattacac acctggttta gaagtgctta aaaaaccaag agtgtcaaat ttgttggaac 4920

aactattgtc gcgcgtgaat taatgaacca aaaggttaca cataagtgat ggtatggcca 4980

gaacattatt tcaaatgaaa tttgattatg gcgtgataag aatgaaagta cctcatgaaa 5040

caaggatact atttgccatt gcctattgtg atgaagtaat gggtatgggg aaggcaaaag 5100

agtagtatac catattttta taattgtggg aggccggact agtatctatc tcccaaggtt 5160

taggagtctt gagggacgtg ttgttaaggg ccaaaattag tcactcttga ccccaaacca 5220

acacttgtta ttgttgttac ttggaagcct acgtcatgga gatgtaggca gactcttatg 5280

tcaatgtggg cctagagaca cagacccaac aagctgttgg agagcaccta ggtgtgtgca 5340

aagtagatgt actcacgtgg atggatgtgc tagccaaggg ttggtgtagt gcacatgcac 5400

aacaaaaata tttgttgaca ataacaacac gtaaggaaag attgccccat tgtattgcac 5460

taaggagtat ctcatgggca ggccaagaaa cacctcaaaa agttggttgt tctatacatg 5520

ccatcgagtg ctaggacaca tgtttgtggc catatgtatc ttgtggaaag tgagtctaga 5580

tggttacaca tataattcaa taatataaaa acgaggtttt ctaacctagg catgtttaaa 5640

tgcatcctca catggagtcg agcctaggac ctaaggtgct actaaggttg ttgcacccac 5700

tgggctacaa accctttcac cctataatct attgagaaca ggaaattgat taagggatgt 5760

ttccacaaga gtaaatttta ccgtcagccc ttgaacttgt tctatgttct catctcggtc 5820

ctagtacttg caaattgcac atttagctcc caaaacttat ctcgaattct tagtgttggc 5880

acttgcgttg cacgcttggc tttctaaact tgttctatgt tttcatatcg gttccagtac 5940

ttgtaaattg cgtgctcaac tctctaaact tatattactg taccatccag gtccaacccc 6000

tgcacactat gttgtcctta cccatcactc cacaagtgca tgtctcacca acgcggctac 6060

tctgtgcttc accatgcttg cctgcctacc tgcgttgcat caccgacaca atgtcatact 6120

accatgccac aatgatgcac taccatgttg tgcccttgcc gaggcgcctc actcccatgg 6180

tggtgtaggc gtacgtccac ttagtgagct tgtcgcgaca agagacgagg aaggttggaa 6240

gggcagagag ctggggtgag gagatcatga tcatgagagc atgcgcaatg ggtgagagaa 6300

aggagacaag gagtgtgtca gtattggagt tgagtgcaat taccgcgggg gcaggggtaa 6360

cgctggtggt gctgcaaggt gtaggagcga gtcgagcaag agcaaagagg gcactgaagg 6420

taggatcatg cctagacact catcttcgag aaagctaggc ttgcacgatt tggtggtgca 6480

aatgagcatg gcaatgggtg tggcaccgcg tcaacaacgc agcgtaggta agcatgggat 6540

ggtagattga tcacggtgca acacgacgca ggtgaggtgt gcacatgcat ggcaatgggg 6600

taaggacggt gtcatgcagg gcgaggggtg gactgggatg gtatagtaat acaatttttt 6660

ggagctgaat gtgcaattta ctagtacgag gatgggaacg agaactcata agtttaggga 6720

gccgagtgtg caacttacaa gtactgggat caggttgaga ccccttaagc ctgcacttat 6780

attattttaa aagacccttt cctagcattt gtttcatatg gaggataatc tcccaagttg 6840

tgtattatct attgaaatgg tgcaactttg ttgttttgag ctgggaacta atttcacttt 6900

ctaaaatctt acatatataa tattttatgt tttctttgat tgtttagtgt ttaatattta 6960

gggtggataa tacctgcttc aagctttggt acatagggac aattgcaaat agaaatggag 7020

taggactttt gattgatacg agtctcaaga acagtgtggt ggatgtgagg aggcaagaag 7080

ataggattat ctttttttct cgaacacgca gaagaactgc acatctttat attaagaaga 7140

agaagaagaa agagaggtcc aaaatagacc gctataaagc caccgaaagg tagcaataat 7200

agttacaaac taatcacctt gagcaacacg ccccctacaa caatgacatc ctctaaggcc 7260

ataagccaac caacccaaca acactagcat ggcaccaaac tcccatttag gattggattt 7320

tgcgatagct tttgacctgt tgatagctaa cacctttaag aagagagact cccatttagt 7380

gacctatttt tagataatgg aaacatagtc cgaccttggc ctcaaacgag gttacgacca 7440

attattacaa ctgaaggcaa catattgcct tgacctatca cacgacctca ccaaagaaca 7500

ccaactacta aatcaaagct acaactaagt taaacgcaac gaagaccccc atccaaaatt 7560

gtgaaagaac tgtagtagca ccaactccac atgacgacat gaagacttaa ttaattcttg 7620

gtcctccctt ctaggctgaa gctgtgtcta gaagtgacac caataagttg tcctgaaggt 7680

tacctgcaaa caagatttag gggacaattt attgaaaaca cctcattcct acttaaccaa 7740

atagcccaac aaattgtcga tgcaatggtt aatagtaagt tgcgtgatct ggacaacagg 7800

ccactcgccc aaaccccaaa caaatcatta gcatcccgag gaggtctcaa tcccgtactt 7860

atttgtaact aatgccaaat gaaagacaca tacaaacacc tgaaaaacag gtgtttcact 7920

gtctccggtc tgttacaaaa gcaacatgtc ttactaccgt tccaattcct cctagccagg 7980

ttatcctttg ttaaaattac acctcttttt aggtaccaca taaaaaaatt tattttcaaa 8040

ggaatcttaa gtttccaaag gtccttgtta tccattccca ccccattgtt gagagttgcc 8100

tgatacaaag actgaacagt aaacaaccca ttggtagtct agttccatct gaacgaatcc 8160

ctattagaat tcaaattagt atgtgctacc agcgatacca gttgaaacca ttggaccccc 8220

agatggtgaa ccaaccccct acgaaaggat atgtttaagg gcaccgtggg cagattgact 8280

ttgtcctcac aaggagagaa gataaacgac catccttgga ttgtaaagtg atacctagga 8340

agcgtgttgt ctctcaacat aagtttgtgg tggcggtctt ttgttttcag gtgtgtgtcc 8400

atagggataa acaagacaag attgcgagaa caagctggtg gaaattgaaa ggggaggagg 8460

gaaaaaagtc ttcaaggact aaaagaggac gtttggaaag aagagatgac acaaacaaca 8520

cgtgggagaa gatttttctc gaaagcgcag gagagctgcg cgaaaatata ttaagaaggg 8580

gaaaaaaagg tccaagaagg accccaagat acagataagg ccgccctatg gcggccaaaa 8640

acaagcataa atgaaaccat ccatgacaaa aacactgcta ccaaaacagc actacaacta 8700

tctagctaac aggtagacct gggatggggg cagtaagcaa ggacaacttc tttgcaccag 8760

ccataaccca aagatcaatc tccaaaccaa tgcttctaat agcagcagcc acactaggac 8820

tcttattgtc aaaaacgcag atagcaacct acgttcggaa ggtggtctca gaggtatgtg 8880

gagtaactaa ggggagtgga ggtgaagcta aagatacttg gtggttaaat gaggaagtcc 8940

aaagagctat taaggagaag aaagaatgct acaaacgcct ataccatgat aggaatgtgg 9000

acaacataga gaagtacaag gtagcaaaga agactacaaa gcaagctcta aatgtggtaa 9060

agggtagggc gtatgaggat ctttaccaac gcttgagtac gaaggaagaa gagaaggaca 9120

tatataggtt ggctaaggtt cgtgagagga agataggaga ctttaaccat gtgaagtgca 9180

ttaaggatga aacagagcac cttttggtga aggaggatgg gaccagacat agatgccaaa 9240

agtattttaa caaattgttc aatggggaca caacctttta gttgaatgac ccttttgatg 9300

acaccataga cgctttgtgc ataggatcca agaatctaag gttacagatt agggaggcat 9360

tgaaaaggat gaatacgagt aaggcgatga gccttgatgg tatcccaatt gaggcatgga 9420

gatgcctcag agacatctgt tgtatgacta accaacttgt tcaaccatat cttccggttg 9480

aacaagatgc ctgacgagtg gagagctgaa gaagtatatt ggtaccgatc aacaagaata 9540

agggagatat ttaaagttgt actaattgtt gaagtatata agtggattgc ctaccttcta 9600

acaatttaat cttttgggtt gaactggtta gtgcgtccac tctaacatgg tatcaaagtt 9660

agaggtctcc taacaattcc atgtttgtcc ctttaattgt ccttctgcac ctacacgtgt 9720

tgaactgtat aaatggattg ggttgaactg gtaagtgcgt tcactctaac atggtatcaa 9780

agcctaaggt ctcgagtttg aatcctggcg gatgctttat ttgtgcctcc gcccctttat 9840

tttcatgttt gtgccttttt ctatagatgc acgtgagtgt gggtgttgaa atgtataaat 9900

ggattgccta ccttcttcta acaacttaag tttttgggtt gaactgatta gtgcgtccac 9960

tctaacacta attacagagg aattaagttg atgagccata gtatgaagct atgggagaga 10020

gagagttatc gagtatcatc tgagaggaat aacgtagatc tctttgaacc aatttggttt 10080

aatgcttgga aggtcaacca tggaaaccat tttttaatat gacaaatgat ggagcaatat 10140

atggaacaaa agaaggatct acacatggtt tttattgact tagagaagac ttatgatgaa 10200

ataccaagta atgttatgtg gtgggctttg gacaaacata aagttccaag gaagtacgtt 10260

ggctcattac ggacgtgtac aaaaatgttg tgactagtgt tcgaataagt gatgatggca 10320

tagatgactt tccaattaga atatgattaa atcaagaatt agctttaagc ttttaccttt 10380

tgccttggtg atgaataagg tcacaaagga catacaaggg tatgtttttt tttacgaatg 10440

atgtagttct agttgatgac gggcatgagt aaatagaaaa tagagttgtg gcgggaggct 10500

ctagaatcca aaggttttag acttactaga actaaaatag aatatatgat atgtgatttc 10560

gacatacaca tgatgaaggt gatgttaatt tggaaggtca agtagtccct aggatggaca 10620

cctttaggta tttaggatca atgctacaga gtgataggga tattgatgaa aatgttaagc 10680

agggtggatg aagtggcatc aagcatctgg cgttctatat gacaagaggg taccacaaac 10740

ttgggcacct cctaagtgtc ggttcttcct ttggctggtg gctcataata ggtgctggac 10800

tgcagacaga cttgaaaaaa gaggactaaa tcatcctcct agatgccctc tttgtgacca 10860

agagcctgaa accatcaacc atcttttggt ttcttgtagc ttttccagat tatattggta 10920

catcactctt cggaaattcg ggctccacat tcttgcccct atccaagatg acacaatctt 10980

cctgcagtgg tggaaacgca ttgctgatat tgtgtcaggc atgaccaaga agggggtaaa 11040

ttgcgttgga tttgtggccc atataagaag ggatcgagtt cagaataatg atatacatga 11100

taggctaggg gtagcgccaa ttgaagaaaa gttgatgttg atccaacatc ggttgaaata 11160

gtttggatat gtccaataga gacctctaga gacgtcagtg catagtggga tcctaaggcg 11220

cgataacaat gagaagatag agaaagaccg aagttgacat gaaaagaagc tgtaaaagga 11280

gacttgaaag gatgtgatat attcaagatt tagtcttagg ccctgtttgg cacagcttat 11340

tttcaacttc ttcacaaatt taagcagagg ttctgccaaa caggcagctt ctgcagcagc 11400

ttatcagttc agctgcttct cagaatacac taaaagcagc caacaagcag aagctgcttt 11460

tcaccagctt ctcagataag ctgctttttc aacaagcacc agctgtgcca aacagggcct 11520

taataggagt gcatggaaaa caactattca tgtgcccgaa tcttgattgt gggttctatt 11580

ggatttttaa ctctagccta cccaacttgc ttgggataaa ggccctgttt ggcacagctt 11640

attttcagct tcttcacaaa tttaagcaga agttctgcca aacagctagc ttctgcagca 11700

gcttatcagt tcagctgctt ctcagaatac actaaaagca gccaacaagc agaagctgct 11760

tttcaccagc ttatcagata agctgctttt tcaacaagca gcagctgtgc caaacagggc 11820

caaaagactt tgttgttgtt gtttctttga ctgattgtta ttgttcttta agaaatttag 11880

ttatgctatc caaccttttt cagttatctc tgccgaaaat ggccagcaag catgggctta 11940

tcttgaagat aagagaaaca acatagatct tgttttgact gaggttttta tgcccggtgt 12000

atctggtatt tctctgctga gtagaatcat gagccacaat atttgcaaga acattccagt 12060

gattagtaag taactttcat ctctacagtg tagttcatat catgattaaa atgttgcttt 12120

catcatttat ttagtcccaa tgcttttagc tgctacactg atgttgtttg tgtgcagtga 12180

tgtcttcgag tgatgctatg agtacagtct ttaaatgttt gtcaaaaggt gctgttgact 12240

ttttagtcaa gcctatacgt aagaatgaac ttaagaacct ttggcagcat gtgtggagac 12300

aacggtgtca cagtgtaagt tgttcatggt ctggtttgaa tattgtaagt tctttgacgt 12360

tattcatgtt tctctgggta aaattgtata attactctaa caagagttta cttatttggg 12420

aaacaagaag acacctattg aggtatggtc tggtaaaaca taaggctgtc cctttagacg 12480

tgatgagctc tcccgctccc agcccctctc cctcccccag cccctctccc tctccccctc 12540

acacctccac cagcctctcg ccggaatcca ctccggaccc gtcgaccaac tcttcggccc 12600

ccaccccctc ggagattgcc cccagtcagg ccagtcgccc gatcccccag gaggcaggac 12660

gttctaagtc ccagagatgg tgtgatgacg ttattgatgg tgactcatcg ggaggatcct 12720

cctctgctta tcattcctat aaagaagctc tccttcaacc ttctggtcat ggtggtaacg 12780

cccctgctcc ccctattccc cacttgaaga cgaaagctgg tgaccagtca gaggtgcaag 12840

ggtcttctgc tgaaagtcga cgtcgaaagg aggatggctg gcaggtggtt gagcatagga 12900

ggcgccacct tcctcgtcga ggatccatag tggatctccg agggaagtgc tacaagtgcc 12960

tttctacctc ccacctggta gcttcctgcc gacgtcctac gaggtgctat cgctgcttca 13020

agtttggcca tcaagcggca aggtgcccct tgccggaagc tgggcgaaag acagtgtggc 13080

agcggctggg ctctggtgga gatcgtactc cggtgtggct gcgtttgtct ggagtggaca 13140

attctggccc aacggtgatg gatcgtccag tcattcagcg caaggcccca gtctggcgaa 13200

ggctttcccc tccagtgaac tccccattag tggaaaaaga agcacagggt ggcatgcttc 13260

tgcctgttgg acgtatgaag aagaagagaa gacgttctaa acgcgggaaa ggcagctcgg 13320

gactgcagtc gggcccaagt cagccgcatc ccaatccccc tgttgctgtc cagagcgcct 13380

tgccgcggaa accttcctgc gtattggagt tttccactga tttggcaaga aaggaagctg 13440

ctcttcggag agccttgttt gtttcggtgg ttggcaccag gccgatggtt cgcggggatg 13500

aggtgattga ggaattggcg cgaagtttca gcatcaaccc tgatgatatg tctatccagc 13560

aggccacgcc ggaagatttc ctactattcc tgcctgatga acacacagcg acccaaattc 13620

ttaacgaagg acgacctttc agaggccctg gattttctct caattttaaa agatggacaa 13680

gattctctca tgcctcgacg acgtctatgg cggcactggt tgatattgag attcgaggaa 13740

ttccagctca tgcttgggat atctccacgg caaaaatgtt actacaggat tcgtgctggg 13800

ttttggaatt gttaagggat tcaggaacag catttctgct acgcgcctgg tgttttgacc 13860

caaaatatct gcattcagaa atggaacttc atatcgtcga gccgggcagt gatgagcaga 13920

agaaacgatg tttgacatac aacatttcca tgacagtgtc atcggtgccc tttcagatga 13980

ccaatagcac tgaaactcta tccccttcct ctgacgatgg tcagtttgat gaaggaaatg 14040

acagtgatcc agctgactcc cagcagcgcc gccttccacc tcagccacct agggaatctg 14100

ttcaccttcg gataggcgcc cagtttgtgt ctggaaggag tagggcgctg tgttcggttg 14160

aggaagctga aaggatgggc ttaggcgcct cttcgggact taatgcacaa cattcaatgc 14220

ggccccttga agattgcccg acctggggag atgagatggg aatgcagctg gtctgtggcg 14280

cctcgggtcc ttccaggcag atatgtggcg ccttgggtcc ttccaggcgg atatgcggcc 14340

agccgtccat tgatgcttgc gttgcccaat taaataacgc acagctcgat gctcgggccg 14400

gctccttcat atcggtggag agatcccagg agtcgccttc cccttcatgg aagcagtgta 14460

gacagttggc taatgaggca cgcgaccttg gggtccgagc tgcctccttt aatgatgaag 14520

aaagaaccaa ggtgccacta gctgttgggt cgctgaatca gttgaggtca gcctcgcccc 14580

agtaccattt agctgctggg ccgctggagg gggaccggac tgattcgccc caagctactg 14640

ggcctttggg atttacgggt gaagcacgcc aggatgatct tgtggctgaa ccggtggatt 14700

gggctacgac ggacctcacc catcaaatgt gcatttctga gactgagagg acgaccttga 14760

ctctgggcga tcatgtggct gcggacagcg gaaggaagtc ctcaaccggg aatttgctac 14820

accaatccat tcactgcact actggcattg aacagccagg gatgctacat attaggggcc 14880

ttcaatcatc caaaaagctg atggtatact cacgaaaaag gttcaacagt agggacaagt 14940

ctggaaatat gaatgaagaa gggagtgggc caatcgcttc tgtactgagg attgatactg 15000

atgaaaatac agtgtccacg gtgcgagagg tcagcctagc tgttactact gatggtagtc 15060

agtcaacttc gcaagagacc caaacaggca gtacgcagct caagaacaaa caattcctta 15120

gcaagctctc caaaaagatt tcaggcctcc ttgcaacacc agaagatccc ccttttatga 15180

cgtctaaaaa tgcccaagtt gcagaaatta tcccacgccg cagccgtcga attgctggcg 15240

tgggagttga atttgacaac atggatttat cctcaagaac cacaaagaaa gttatgcagg 15300

ccttgaaagt aattggggat cctggggcag tcacccagca agccaaagaa gactatctga 15360

aagtattctc cactgaactt cctatttcac atattgaagc gctggcgtcc ttttttggat 15420

gggtggtacc agatgatctc aaggtgggca gcattagtgt tggcattagt gcctgttgag 15480

ttgtcctgta gaggtcgctg atgtaagagg tttgtaatgg atccaaaaaa aatcttgagc 15540

tggaatgtaa ggggtctcaa ttctacatct aggcaatgtt ctgttagaac ttgcattgat 15600

gcttgtggcg cagacgttat ctgtcttcag gaaacaaaaa tgcaggaaat taacagagga 15660

aaggtgctgg ctattttggg aacagatttt gatcagtatg taatgctgcc gtccgagggt 15720

gctagggggg gaattctgat ttcctggaaa aagcatataa aggtgacagg gctgaacaga 15780

gtggattctt atagtatctc cttgcaattc cagagaaata atgggcagtc ttggtggctg 15840

acttgtgtct atggacctca aagtaattct gaaaagatcc tttttcttca agagttaagg 15900

catttcagaa gcatctgctc tgagccttgg gtaatagtgg gagactttaa tctcatctac 15960

aaggttgaag acaagaataa tgataatatt aatagatcta tgatgggtcg cttcagaaaa 16020

tttatcaatg atgttggtct gattgatact cccctaattg gtcgcaaata tacctggtcc 16080

aatcatcaat cttcaccaac actggtccga ctggatagag ttctctgcac tactgattgg 16140

gaagcgttgt tccctaacca tctccttcaa agtgctgctt cagaggactc agatcattgc 16200

ccactgattc ttggacttaa cagtatgtgt tctggcaagc gtcgatttca tttcgaatcc 16260

ttttggccaa aattggaagg gttccaagag gtagttctgg aagcctggaa ttcggttaat 16320

gaaagaagtt gccctttcct gacattcgaa ctgaaattga aatcaactgc caaggctctg 16380

cagagatgga gtgctaagaa ggtaggccat gtgtcctccc agttggcact tgctagagaa 16440

attcttcatc agctggagat tgcccaagac tctcggctcc tgtctccttt ggaggtttgg 16500

ctcttgagag ggcttaaaaa acactcctta gcccttgcct ccctcaaacg cactattgcc 16560

cgcctacggt ccagaatcag ttggataaag gagggcgatg ccaactctaa gcttttccat 16620

atgcatgcta ggcacaggca aaggaagaac tttgttgcca agattgtgtc agggatgaac 16680

actttcactg atcacggcga gaaggctaga gcagtagatg agtttttatt tcaaactttt 16740

gggctgcaag gcggacagat ccttgactgt tgatttggat tacctgggtc taccttctca 16800

tgacctgact gatttagatg cgcagattga tgaaaatgag gttctggaat ccataatgca 16860

actaccttct gataaggctc cggggcctga tggttacaca ggcagatttt ataaagtttg 16920

ttggccaatt atcaagacgg atgtgatggc tgttattgct gcaatttggt gcagaaaatt 16980

ccagcagttt ggaagactta acactgcatt tgttactctt atccctaaga aggagggggc 17040

tgaggaagta aaagactttc gacctatcag tctggtacac agcattgcta aacttgtcac 17100

caagttgatg gccaacagat tgtcccaacg actgcatgat cttgtttctc ttaggcagag 17160

cgccttcatc aagggccgtt tcatccaaga caacttcatg ctggttcagc agactgccag 17220

attgcttcac cagcaaggcc aaccgagggt tcttttgaag cttgacatca ctaaagcctt 17280

cgattctgtt tcttggcctt ttttgcttga agtacttcag agattgggtt tcgggcagat 17340

ttggagggat atcttaagtg gccttttagc aacttcctcc acacaggttt tactcaatgg 17400

agttccgggg aacaccatcc cccacaagag agggctgcgg caaggggatc ccctctcccc 17460

tatgcttttt attttagtga tggacatcct tggttgcatt gtggccaaag ctgatgagga 17520

ggggcagctt gagcctctct cttccaaacc actgcaccac agaatttcgc tatatgctga 17580

tgacgcggtt ctttttttcc acccaaggga gagtgaaatt catactgtcc taggtattct 17640

gaatttgttt ggagaagctt caggattaaa aactaatatt cagaaaagca gtgtgtatcc 17700

aatccgttgt gggcaggatg agctggacat cctcaatgat tggcttcctt gtgaactttc 17760

gtctttccca tgcaaatatc ttggtctacc tttgtcctta aaaaagctgt ccaataatca 17820

gctgcagccc attattgaca aagtggctga tcaactttct gggtggaaag cggacctcat 17880

gacacgggca ggtagaagag tgcaggttca gtacgtgatg actggcatgc ttatatatct 17940

agtcatggca gttgacattc ccacaggctc gctcaaagct atcgacaaga tcagaaaagg 18000

atttctctgg cgtgggagga aagaggtcag aggaggacat tgtttagtgg cttggggaag 18060

agtttgtaga cctcttgaaa ttggtggact tgggatttct agtcttaaag aactaggatg 18120

ggccttaaga atgagatggt tgtggctgcg caaaacggat ccggataagc cttggtcctc 18180

attgcccatg cagatatcca acaaaataaa ggcctttttt tgcaccgccc tgctcactga 18240

aattggaaat gggtcctcaa ccttattttg gctggatcgt tgggtccacg gaaagaatat 18300

tgaagatatt gctcctcgac tgcttgcagc ggtaccaaag agaattagga acagcagaac 18360

tgtgcttgag gccttgacag acaggagatg gctaactgat attaaagggg ccttgacagt 18420

aggtgtcttt gccgatctcc tagatctctg ggatgaactt caaagtgtcc agcttcattc 18480

agacagggag gacaaacata ttttcaggtt cgctaatgat ggaatttatt cagcgaaagc 18540

agcttacaat ggcctcttca tcgggtcgac catggctaag tattgggaac taatttggaa 18600

aacctggtcc cctcccaagt gtaaattctt tttatggctt gctgatcttg gaaggtgctg 18660

gacagcagat cgtttgcaga agagaggtct tagccatcct gacaaatgtg tcctgtgtga 18720

ccaagagcaa gaaaccatag atcacatttt ggtggggtgt gtttttgcta ggagtttttg 18780

gtttcagctg ctgggacagg tcaacttatc tgcctttact cctgtgatgg gagaaactaa 18840

aatgatggag tggtggagca gaagcagtga gcagttacag gggattgcta aaaagggatt 18900

caattctttg attacccttg ggctttggac tctctggaac cacagaaatg gatgtgtttt 18960

tgatagagct acacctagca tggagggtgc tatcagaaga gctgaagaag aaattattat 19020

ttggaatttt gcgggggcca aacatcttgc ccttattaca gctcccattc caggtgtcat 19080

gtagggatct aggagtgagt tcagtagtta acctcactag ggaagggtgt tggttggtta 19140

agtttagatt gtgggggtag gttggtctag tttgctttct ttcttctcct ttttcttttt 19200

ctttttcttt atatttcttt ttctgttgtg tttgtaattg gtctaatttt tagacccttc 19260

ttcttaatac aatgatacgc agctctcctg cgtgttcgaa aaaaaaaaaa gtttacttat 19320

ttggaactca gtccagtggt agtggaagtg aaagtggcat tcagacgcag aagtgtacca 19380

agtcaaaagg tgccaatgaa tccggtaaca acagtggtag caacgacgat gaagctggca 19440

tgggacttaa tgcaagggat gacagtgata atggcagtgg cactcaagta tgggttttga 19500

tacttttttt tacttctcta aagcttatta ctggctgtta tcatgttgtt caaggtatga 19560

acgtagtaac acttagaata caagaaacat tttggcgcaa ctgcctataa tcaccaaatg 19620

gaatgtatta gaggtcagtt cacatagcag ctaaatttaa ttgtcattta taaccacaga 19680

attagacagt gtattgctgt cactactaaa tgttaatttc ttaggataac tatttctata 19740

tatatgctag aggtaccaca ttcctggttg aaacaaatga gtaaattact ctggatctta 19800

atttaattct aaaaaattgc acatgaacta gagaatgcct gtgatgtggt tttatttcac 19860

ctaacacact taagtccatc ttgcctctgt tctattttta aacttgacct gcggggggta 19920

agacagcccc cgggcattgt attaagaaca agaccttctc acacaggtcg agaaaacccc 19980

cgaaccccta ccccacccat acacagcggc atcgaagccc atgtgagaac gaccacgacc 20040

ggggctgagc cttgaacctg tgctttagcg tgggacagac gaggggattt ttttaaccac 20100

agcttgaaat tcgctcccac ggagagtcaa acccaggacc tgaggagtgc tactcagacc 20160

acctaaccaa ctcagctaga ggccctttcg ctctgttcta tttttaggct aaaagaaaaa 20220

tgccgactgt attatcttac tgtttcttga acacacatga gagatatgta tcttatatca 20280

ccccttgtat ttttatacaa ttatatgtta gtttagttgc atccagactt ttgcttaaat 20340

agtagcaagg gttcactgta accagttctt tgttcagaat ccatttagaa atatttggtt 20400

gtggaaaagc ttgaggtgaa ctgtttggtt cagaaacaaa ttattgtttc atatccacag 20460

tccatgagtt ttgacgtcaa aagctcatac tgatagaaaa acagagaccc actggatcag 20520

cctaagtccg acacaacctc aatgatgata agggccctaa ttctaatctg aagggttgag 20580

ggggaaggga aaactagcat acttactgtc caagagtaat ggcggcattg ggcatcgccg 20640

tcgtgcccaa ctgcccatcg ctggtgctcc acctccctgt tgtcctcctc cattgtcctg 20700

ccccatgatt agctggagaa agtgggggct cctcggatcc cggccctgtg ggggtggctg 20760

ggctctatgg acttcatcga ttgtggctct tccggtcgct gagaacgccg ggaattgtgg 20820

tggctagggt tttcgggcgc tagaatgtgt tggcgtctct gctggcctag gcctcctatt 20880

tatgttgtgt tctcgtgacc cggggggggg gggggggcaa ccataggttc ggtttgtgcc 20940

cccgatcagg gcgtgcgaca ccatgcactt ttggggagtt tggtgcttat tcaacttggt 21000

tctgttgctg agtcagtgaa catcctaaca aatacacgta agaaccaagt ttgaaatttt 21060

cacgtttgcc actgatgttc tcaatgccat ttgtccacat gagaacattt tgtaacttag 21120

gagtgatatt tactgtatct tttaaacata aaaaatgttt ttaaacttca gatatgttaa 21180

gttaatttta cttattaaac ttgtcgtttt caataatctt ttgctccttt tgtaaatttc 21240

ttttgaacac cgcttgcatt ttaataaaat gtagtttctc caatgtgttg tgaaaatgca 21300

tatgttgtgt tgaatttgtg gccatacaag aagggatcaa gtttgaaacg atgatatacg 21360

tgatagacta agggtagcac caattgaaga aaagttgttc aacaccgatt gagatggttt 21420

ggacatgttc aatggagatc tctagaggca tcagtttttt ttcttgaacc atgcaggaga 21480

attgtgtcat tttatttaag gaggaaaaat aattacatcg ggtaggcaaa aatgccccac 21540

ccgaacgcca cagacaccca acatgggact actcaaagag gagtcacgac ccggaccaat 21600

agccctaccc agctaagacc tgcgaccttc tattttgctc taggaggttg gcagctccgg 21660

ccctacacca aagcaaacct tcgtcatcta ttaactggag aacccgccga acatctggct 21720

gactggcctc aaacacaaaa gagttcctga atttccaaat ctcccaagcc actaaaatga 21780

acaaaaagtg ttgaaccctt tccgcacttt cttatgcacc atcttgatgg agcgtgccac 21840

taggaaaaga aatgagtatc agctgtagaa ggcgtaagag gggcaaggtt tagggagtga 21900

aggatcatgg tccagatttg tcgtgagaac acacagccca cgaagatgtg ttggatggtc 21960

tcgtccattg ttttcaagtc gaacgacttc ctggtcattc tggctgaccg acttgggcta 22020

ttaatcgtga ttaatcacga ttgatcggac gacttggacg attaatcgtg atcggtccaa 22080

actgacttgg atgattaatc acgattaatt ggacgacttg aaaacagtgg tcttgtttgc 22140

ctgatcacag aaggtatagg ccatggggta tgggagacct cacttggcaa ggcgatccac 22200

cgtccaacaa cggtgatttt ttgcaagcca aaggaagaac atgtacttca agggagccca 22260

gcctttccag atattcttcc aaccattaaa actgacagag tccaaaaaga acatggagta 22320

ggctgacttg ctgctataaa ttccggattg agtgaacttc cagtggtgtt gatcagacac 22380

gccctgctgg agaggcatgt catcaaccaa gtcccaaatc actggatatt caatcagcac 22440

ttgcacagat agagcacctc tgatgtcagc aacccaggtt cttttttttc tcgaacacgc 22500

aggagatttg cgtatcattc cattaataga tagaagaagg tacaacacac cacacacgac 22560

acacacctga cacgacacac acacacatga cacaacaaga cacaagacgc aacacagcaa 22620

ccacacacaa ccactctgaa caagccacta gactatcccc ctggtcaagc ataaacataa 22680

gagttgaaag caggttcaac cctttcgccc cggcactaca ccacatcctg agctcatcgc 22740

cagcgagggt gagagcactc gccaggctcg gcgatctccc attaaacaca cagtcgttgc 22800

gatgacgcca aaggctccaa gctccaagaa taactagaga attgaagccg gattggagat 22860

cacggggaat cttctccaca gccctgctcc accagtcatc aaggctgcaa ttagcattat 22920

ctggtgaaag gttaggcaac ccagacctgc ttaacaagga ataccagaac tgtcgtgcaa 22980

agacgcaaga gatgagaaga tgttgtattg tttcctcttc ttggtcgcaa agggggcaca 23040

cactggggtg gggtagcccc cttctagcaa gacggtctgc ggtccagcat ctgttatgca 23100

tgacaagcca aaggaagaaa ctgcactttg aaggtgccca agattcccag atgcgctcat 23160

atgcgcccaa agaagtggaa ccaagaaata acatatcata agccgacttt gaagagtatt 23220

ggcctgtcgg ggagaatttc cagatatgct tgtctggcac tccaacatta atggagtaat 23280

ctgcaatgag ttcattaaga gttaaaaaat cagagaggac atcccaagtg atggtgcctc 23340

tcaggtcccg aatccaagcc atctccacga caccttcagc caccgttctc ctattggtaa 23400

tcctggtcgg gacgagggcg aaaatatgag gagttagatc ctcaatcctc aaccccatca 23460

accatctgtc cctccaaaat aaggtgttac gaccatcacc aatttctgta atcaaagctg 23520

tagagaggat agatttcaga gtgctggaac tctggagagg taaatcagcc caagacttcg 23580

agtgatctgt tctttccaac cacagccatc taacccgaag ggcccaattc agcttttgta 23640

aatctgagat gccaagccca ccaagctcct ttgggcgtgt cactttgccc caagcgacta 23700

agcagtggcc accgagcaca tccttacgac ctttccaaag aaagccacgt ctaatcttgt 23760

caatggcttt gatagcccac ttaggtaaat ccaatgccat ggcgtgatat ataactgttg 23820

ccgtgagcac atgctgcact tggatggctc tgccagctcg agtcatcagg tcggccttcc 23880

aattaggtaa gaggtccgca agcctatcga ttagtggttg cagctgctcc ttgcttagct 23940

tcctgaggga aagaggcaac ccaggttcta ttgtggagga gggcttgagc cacagtcctg 24000

ttcttgactg ccctctttgg gactgcagag agtagagatg gcgccaaatc agctacgcac 24060

tttccatcga accacctatc tgtccagaat tttgttgaca ttccatctcc aacagtagtg 24120

cgaacaacca tatcaaacaa gacccttgcc tttgacagga caaaaacagg aagcccgccc 24180

aaagtctatt gagaaggttg aagttgatgt gagagactaa aataagattt gaaaagatgg 24240

aatataccca aagatttagc cttgaatagg agcgaataga aaacaactat ccacatgctt 24300

gaatcttgtg gtttatgtta ggttttaact ctagcctgcc tcaacttact tgagactaaa 24360

atgctttgtt gttgttgttg ttgtttctcc aatgtactac taagttatta ctgatatgtc 24420

taaaaggcct ctaacttagt tgattaggtg gtctaagtag cactcctcag gcccggagtt 24480

tgactctccg tgggagcaaa tttcatgacg tggttaaaaa aatcacctcg tcttcccacg 24540

ccaaagcata gttctaaggc ctgcccagtc gcagtcgttt tcaatgggct accgtgccgc 24600

tgtgcatggt ggggtaggga ttcgggggtt ttgtagacct acgtgataat gtcttattaa 24660

tatattgccg tggggcggtc ttatctccgc aggtagagtt ttttctactg ataaacgaca 24720

ttagggaatg ggtactgtat ttacaaaatg aactaagtgc aacatgagat atttggtttg 24780

catattatat cctaccatca cacaactaga cataacctga tatggaaacc aaataaaaga 24840

ttttttcttg cagataataa ctaggtggac taacctttaa taattgtata ccattaccac 24900

ataagcagga aaaaattaca cagtgaagac atagtttggt tatgtgtgaa agacgataac 24960

attatgatat gtggattact tatgaggtcc tcaaaagaac ttatgaggca aaagaagtgc 25020

atattttcaa atttgtagtc ttgaaaacgt gaagctgtat ggtttcctgg caaccttgca 25080

agcacatgta cgtattagga taaactatga tgttttcatg ttgacaggat ttaaacgatc 25140

ttgttggcgc ctttttgggc caaacactaa gggtccctgc ctccctggat cccgctcgcg 25200

gggaacgaga ccttggagaa cctctctgcg agatggtaga taaaatataa gacaaataac 25260

actacctgca atacgctcat gaactttacc tctatgatgg caccttctta ggaccaacta 25320

gcaacgaacc ctagggcctt catgtgaagt gcaagacctc gaattcggct ctgcgaggtg 25380

ccggacgatc tgcggtaggt tgtcggacca tcaacgatag ggcagcggac cgtccgtgac 25440

gacatagaga gctcttgggt gaacctggat ctcgcccctg ggagggatct cgtcaggcct 25500

agggtcttgt tagggtcggc aggccaccca cgacgtctct agatgacgta gagtcgaata 25560

caggtgaaga ttgatatgtg gaaactacga ctaaagctgc actagatcta ctcctaagac 25620

atgaatgata aataaggtaa tgtaaataga ttgattcgat tggaatgtgt tcggtgggtt 25680

ttcaatcggt tgtacccctt tatgtttata gggggaggtc tggacccgtt cctaggcaag 25740

atccaatgga ttcccacggt taggttagga taaccatgca tgggataagt actctcgctc 25800

gagttagtct gatcgtggac tgtccggccg tgcccatgtg ccaaatctgg tgctcaacac 25860

ataccccctg ccttttgatg gagcttggtg aaccaaaagc acctggtact accagaatct 25920

ttcgagaaaa catagattct gcaacttgcc ttgttcccga aatatgaact ctagcccgat 25980

atatgtcatt ggctcttgtg atcagataaa actatttgat agaactctaa tgcacagaga 26040

ccgtttaggt ttgcatcctc ttcaaccata tctacttcat caatatgtca ataggcaaaa 26100

acatgtggtg cgccccagcc taaataagca aacagattgg gcaagtaata cagattcatt 26160

gtcgtatcac ctcacacttg agtatgacaa cacattggcg atgaatagac cggaaagtac 26220

cacgacatcc ctggaggtgg atgagcaggt gaccttggct gcaccacttt tcgggccggt 26280

ttagtcagcc tttgcctttg cacagatcgc ctctttgact gttttattgg ccggtggtgc 26340

aaaacggcct tctccttcat atatttggca agaagttgat caaaagtagg gtcagcactg 26400

ctgagccaac caagtgtctt gggggtgttt tgcttcccag tgttgaagtg tataggtgga 26460

cgaactacct tctcctatca gcttaagctt gggttgaact gattagtgcg tccactctaa 26520

catggtatca tagccagagg tctcgagttc gaatcctggc agaggcttta tttgtgcctc 26580

cacccattta tttccatgtt tgtgtctttc tctttgtctg cgtttgcacc tttctctctg 26640

gctgcacgtg agtgagggtg ttgaaatgta taagtggata aactaccttc tcccatcagc 26700

ttaagctttt aggttgaact ggttagtgca tccactctaa cacccagtac atatttcagg 26760

atgttgtagt ctgacggtct gaggtcgccg ccgcttctag ttgtccgcgg attgtccgac 26820

ctttatggtt ggactattcg catctgttct ggactatccg gccttagtgt ccagaccttc 26880

cggcataccc aggataggtg accgagatcg ggtatccgat cgtgcttccc cccggtgctt 26940

tcagacttat tagcctttct tttgtccgga gcctttcgac taaccactcc acgtgataga 27000

tttggcgtgc gagggtcacc aataataata ttttcgcctt tgcctttatc ggccgcacac 27060

ggccgaacca agactttctt gtctgtcggg ctctaatgtg ttgacaggga catgttgcct 27120

gtcaattttt atttcttgaa atctcaatcg accttcattt atagctgatt gtatttaccg 27180

acggaagaca ttacaatcat tggtatcatg aaaaaaggag ccaacaatgc acacgccttt 27240

tcaatttcta tatcggagga attgtgtgag acaatttaat gttaccattc ttaagcaact 27300

catcaaaaaa attatcacac ttagcaacat taaatgtaaa cttaacttct ccccaccgat 27360

tcttttgaat cggctgtagg gaagagcaag cagaaagttt agccttagca agacaaataa 27420

gcttagcggg atgtacctct tttggctcgt catctgagct actactgcca cgacgctcat 27480

catgacaaga ctgttctttg ctttggcttt cacaagtcaa agcccgctgg tgcaatttag 27540

ttagcgaaaa aactagatcc tgtctaattt ttctttcatg tatggacata acacattgaa 27600

agccaaccct gctagttgct tgtccacgac ctggattcga gaacactggt ttctagtatt 27660

ccagaatctc tagatataat ctttaataga ttcatcgtcc tctttctgga tcgatgccag 27720

atcaactaaa tctaactcgt aatcactaga gaagaagtgt tcatgaaact tatgctataa 27780

atcatcccaa gaattaatcg agttaggagg taacacatca taccaagcga acacagtgcc 27840

agtaagggat agtgagaata ggcgaacacg aaaagcttct gtgtcgtcca attcccccaa 27900

ttgtgctaga aattggccta cgtgttcgtg agtgccttta ttgccttcgc ttgagaattt 27960

ggagaaatct gacatactag tccccggagg atatggaacg gtgtcccatt gagggtcgta 28020

tggcctctga tgggactatc ctaggccgga tacactgaca cctaatttat cccgaaattg 28080

ttcgactatt tcttctcgaa cttcatccat agcgatcgat ggtaacccat cggtcctcgg 28140

gacatggggc tcatgtggtc ggttatcctg atgcgggcct tctgcactag gacgatcaaa 28200

ggtgacgttc ggtataggcc cctgaggcat acctgtttca taccccattg tcgtttggta 28260

tgaggtgaac ctttctggct ccctagggat cgaaaaatat tcaacctcat gcatgggtga 28320

tggcatgcta tgactacgat gtgctgtagg tggggtgtag tgtcgctgag gtgagtagga 28380

aaattgttcc tgacgtgcaa cactctgtcc tgtgtagatg tactcggacc gtccgacctg 28440

ggtggccaga cgatccgcga agggactaga cgacccagta ctgtacccgg actgtccagt 28500

tgtgctggca tctcatgcga gccatgtggc gggctcggtg tggctctgaa ctgcccgacg 28560

taaggtgctg aaaggtctgc tatgaaatcg gactgtccga ggttatatct ggactgaccg 28620

acagtatttg gcaccgcatg tgtgccacat ggcgggctcg cgttgcactg gacagtccga 28680

catatggagc cggacagttc gtgcgatggc cagcggagtc agaaagttca ccgcctaata 28740

gatctgactt aatagatccg atctgatgga tctgatctaa taaatctaat ctcagtcatc 28800

cgcagcggag tcaccaaaaa gtgtgttggc gcctttttgg gccaaacact aatgatccct 28860

agaccccgct cgaggggaac gagaccttgg ggaacctctc tacgagatgg tagataaaat 28920

ataagacaaa taacactacc tataatatgg ttttgaactt cacctctatg ctagcacctt 28980

cttagggtca actagcaatg aaccctaggt ccttcacgtg atgtgcagga ccccaagttt 29040

ggctctgcga ggtgccgatt ggtccgcgat aggttgctgg accgttcgcg gtaggcacca 29100

gaccgtccgc gatagggcgg tagaccatcc acgatggtgc aaagagctct tggatctcgc 29160

cccccgggag ggaccttgtc agggaaagat tctaaggtcg tcatggggcc gacaggccac 29220

ccaagacacc tctagacgat gtggagtcga atagaggtga agattgatat gtggaaacta 29280

cgactaaagc tacgctagat ctactcttaa gaaatgaatg ataaataagg taatgtaaat 29340

aaattggttc gattggattg tgttcggggg ttatcaatcg gtcgtaccct ttatatttat 29400

agggggggtc ttcacccatt cgtaggcgag atccaacaga ttcccacgaa taggttaaga 29460

taaccatgct tgggataagt actttttttc tctccgaaaa atgcaggaga gttgcgtatc 29520

attatattaa gaagaagaaa aggtgaggta aaaagagaac ctcatacaac tccacgcaaa 29580

acccacacac actataatag atgcagattt acaaaaatag acagctagac ccgtgacctt 29640

gaagacccca aaccagaggt caccgattgg tgtgctggag cgctaggatg ttagataatc 29700

cacgggctcg agccctacac caaagatgag actcctcctt gacccaattg attaccaagt 29760

tagtacaagg ctggacaccg ttgaaaacac agcggttgcg gtgattccaa atggcccaag 29820

ctgcaagaat gatcagggaa ttcagaccct tgtgcagccc cttgtatgct ggtccaacag 29880

tagaagagga aactccttct caccaatcct taaaagaaga attatcatgg ccaggagcaa 29940

ggtgttgcag cccaacagat ctcataatct gaaaccaaac ttcccgagag aaaacacagg 30000

agactaacaa atggttcaag gtctcctcct gatctcatag caaacagttt tgggggtgag 30060

gtaacccttt ttttgccaga cgatcagctg tccagcagcg accgtgagcc acaagccgca 30120

tgaagaactt gcattttcca ggtgcccaac atttccagat cctcttccaa gggataagta 30180

ctctcgcccg agttaatctg atcgcggact gtccgagtta tctcgcggac cgtttggggc 30240

acatggccgc accgtccgac cgtgcttagg tgccaaatat ggtgctcaac ggatctagta 30300

gggttgggtg gtgttcagag aatgtaagta catttttatg attatcatga ccattagatc 30360

agcttgcata tgtctgcatg ataacttgtc cagaaagtat tgtggtttat ttgttggtga 30420

tggctaccgt gtcaacgcaa cattggtcca tacctttgat ttgaaatatt tgggtgctgt 30480

taacaaagtg tatacctcca agccaaggta gtaaatgttg gacctgaagt gttaaaacat 30540

ggctcagctg gttgggtggt aggtgtggtc gtgcacccaa tcaccgaagg tacaaggcct 30600

cggcttgaat ttgggtgcct atttctctat tttcattttt aattaaaaaa acgtctagtt 30660

tcttctaggt tggatcttga acatatgaat ttcatgattt aaatcatatt atttttcatt 30720

aacgggttat gaacaaggtg atctattctt ataattcgag atgaactgaa ttttgttttt 30780

ctttctttta ctttagttac atagtcatgt atcaattatt tttgaactaa gccataccta 30840

ttttctgtct ctggtttttc tgtggtaagg tatcttactg acaggtgggt ctaagcccat 30900

gtagcactga aagatctcag tggtgaagtt gctatatata tcggaaatta aagcaatgtt 30960

ccccagtgga gttgctatgt atatatcaaa attaaagcat gggcacatta taacttgttt 31020

ccttgttaat atttcataag gcacagaact catggactaa gcttgctgtg gagatcgaca 31080

gtccacaagc tatgtctctg gaccagttag ccgatccagc caatagcacc tgtgcacaag 31140

taatccactc aaagtcagag atatgtagcc acagatggct accaggtagc agcaacagaa 31200

actgcaagaa gcaaaaatac actaatggta tgccatgctc aactgatgca ctatgcaatt 31260

ttacaaaata aataaacttg ctggccattt tatctgttat gtttgatgct tgatttcaga 31320

tgactttaag ggaaaagact tggagataga tggtcctgga aatctatata tggatcacca 31380

atcttcccca aatgagagac ccatcaaagc agcagatcat gaaaacaatt ccaaggagtc 31440

aatgatggga aatttagggg atgcaactgt tcgagctgct gacctaattg gttcaatggc 31500

caaaaacatg gatgcccaac aggcagcaag agctgcagat acccctaatt tctcttctga 31560

agtgccagaa gggaaaggta agaatggtat gtcatgctca actgatgcac tatgcaattt 31620

tacaaaataa ttaaacttgc tggccatttt atctattaag tttgatggtt gatttcagat 31680

gactttaagg gaaaggactt ggagataggt ggccctagaa atttatatat ggatcaccaa 31740

tcttccccaa atgagagacc catcaaagca gcagatcatg aaaacaactc caaggagtca 31800

atgatagaaa atttagagga gccaactgtt cgagctgctg acctaattgg ttcaatggcc 31860

aaaaacatgg atacccaaca ggcagcaaga gctgcagaag atacccctaa tttctcttcc 31920

aaagtgccag aagggaaagg taagaatgac cagcatgata attatgtttt gccatcactt 31980

gagctgagtt tgaagaggtc gagatcatgt ggagatggtg ccaacgatac agtcaatgac 32040

gatgaacagc ggaatagtgc attaaggcga tcgaatctct cggcttttac aaggtgcaaa 32100

acataatatc agtgtcatta gtgagttatg aaagcaacag tatgttgggt actaacagtt 32160

accactttta ttgcaaggta ccatacatct gcggcttcca atcaaggtgg gacaggattg 32220

gtaggtagct gttcgccgca tgataacagc tcggaggctg tgaaaacgga ttctacttac 32280

aacatgaagt caaattcaga tgctgccgca ataaaacaag gctccaacgg cagtagcaat 32340

aacaatgaca tgggttccac tacaaaggat gttgtgacaa aacccagcac aaataacgag 32400

agggtgatgt tgccctctgc tattaaggct aatggataca cgtcaacatt ccatcctgtg 32460

cagcagtgga tggttccaga taatgcgaca gcagggaaag cgaaggctga tgaagtggcc 32520

aacaatgcag gcagaaatag tcatcctggt gacgtgcaga gcaacctgac gcaacaacat 32580

cgtccaaccc ttcattacgt ccattttgag aatagtggat caggtgccct gcaatgtggt 32640

tcctccaatg tatttgatcc cccacttgaa ggccaggcta ccaataacta tggtgtgaaa 32700

gccgggagca actcaggcag caacaagggg cagaataatg ggagtacagc tggtgcgagc 32760

acggctgctg caaatgctgg acggacagat acagagatac gggctatcga taaaagtgga 32820

cctggaggtg gcagtgggag tggcaatgac acatatgtca gacggcttgc tgcgagcatg 32880

acaccacgac aagaacagct aaagaaatat agagagaaaa agaaagatcg gaactttggg 32940

aaaaaggtag cctgttttca attgcatatt aatatttatt ctttatttgt tttcaattgc 33000

atatgtttaa gaagagagag cattggatag aaggactggt ttctttctga tggctgtctg 33060

ccatttccag gtgcggtacc agagcagaaa gaggctagcc gaccagcggc caagggttcg 33120

tggacagttc gtgaagcaag ctgtgcaaaa tcaagaggga gatggagaga gatgaccccc 33180

ccccccctct ggcttcagct catggttgtt agacgtggcg gttgatgatt cttccttctc 33240

tggtgtgatc tgatatgatc gacaacacac catccttcct tttcttcatt ggtttcttca 33300

catctagaat ttactgcctg accgggaaaa aataagctag gaacttctat acatgtgtat 33360

gcatttctaa tcatgtagaa gaataagtgt gtcttgactc tagataattt taggtagagt 33420

catgctcgct ctcctacatt atgtgatgta actgaagtgt tgttgtgttg tcatgtatgg 33480

tcttggatca ctttctgtca atatattact agcccacaac tactattaat ttgatgagat 33540

gtatataatt aaaaata 33557

<210> 2

<211> 20

<212> DNA

<213> Zea mays

<400> 2

gcaggcagaa atagtcatcc 20

<210> 3

<211> 20

<212> DNA

<213> Zea mays

<400> 3

gagtacagct ggtgcgagca 20

<210> 4

<211> 103

<212> RNA

<213> unknown (Artificial Synthesis)

<400> 4

gcaggcagaa auagucaucc guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu 103

<210> 5

<211> 103

<212> RNA

<213> unknown (Artificial Synthesis)

<400> 5

gaguacagcu ggugcgagca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu 103

<210> 6

<211> 33371

<212> DNA

<213> unknown (Artificial Synthesis)

<400> 6

acgaggaggg agcccttgaa ggcaaaccaa tgcgcaccgc cgcttattca ttaaccaaag 60

acttgcatcc aaccaatccc accacttgtg tctacctctt cgagtcaggc tgatgggagg 120

tgcctgtcag caactctccg tggacggaga tgcgaggacg cctgcgaatg accgcgatcc 180

ggccggcctg ctccagcatg atgatacgga aaaccagcag cagcagcagg cctgctggga 240

gcgcttcctc ctcaaggaga ctctcaacgt cttgcttgtg gagagtgatg attcaaccag 300

gcaggtcgtc agtgccctgc ttcgttgctg catgtaccaa ggtttgtttt atcccaattc 360

atccttcagt cgtttttctg acaggctatg atttcgcata ttcatagcat cgatcataac 420

ttggagctgt ttaacatcta taaataccaa ttcgcagcat atagatatgg aacaccgaaa 480

tgataaacat cggtctcagt ttcagtgttt cataaacatt tgcaaagtat catggataat 540

tgatatattg aaacagtaag gcaactgacc aaatcaactt acaatttcaa ctcgcggttg 600

cccgttagta atctaccaca gctggtacat agtagatggc ctcttgcagc cgaggaagga 660

gcgctaggcg tgacagtggt gtacggactg gattgctccc gatcggtgga gcagggagag 720

cggtagcata ggacatcaca agtgatgacg atgccaacgt ggctgtgcaa caagagccac 780

acttggtagt gtttagggtt ccatgatcag ggtgaccaca tcgggtcggc tatgcctatg 840

aaaatggtgc gtgagggagc aacacctatg gaggccacgc tagaggacct gcaggaagga 900

tacatagtgt tgagtcaatg ctgcgacata gattagcctg ttcatgcaag aatgcaaggt 960

ccgttaggag atggtgtagc aaaaggaaaa atggactcat tgaacacgac atgtcaaaag 1020

atgatgatcc tattggattg caagttgagg caccgatatc ctttaggctc cgtttggatg 1080

tcgatattgg agggcaagga attgaattgg gttcaatacc aaatcaacca tgatattgaa 1140

ccaattctat tgtttggatg tcactgaatt ggagtttgga attgtgcggt ctaatccacg 1200

caataccgag gggtgatgct ctgtattgga aagttgagtt tctagttata gtccaattcc 1260

agggaattgg gtctctgatt ccaaacctca attctatgtg caaccaaaca acaaaatttt 1320

gaaaagctga ttccaattct caattttgtg ttccaatatc tacatccaaa cagggtatta 1380

ggccctcatt ggaatgtggg aatttttgct gtttcctgcg tttttcctgt gaaaatgaac 1440

tgattcttgt aaaattcttg tatgattcct ctgaaattcc tgcattccaa agggggcctt 1500

atagtgagga gagtacccaa gaaagacaca taaaatgtac tgagccgcaa gtttatggct 1560

agtagtggca gaaaggttgg ggtaacaagt acaaccaaaa acacgaagat cattcatcgg 1620

tggggagcca gggaaggtga tgcgtggggt ttagtaattt agtgcctgta ggaaggatgt 1680

taagaaggtt ggtgactgta tgcagggttt cagcccagta atgaggagca aataatgttg 1740

ttgatagatc ggatgatgcg tttagccttg ccattttgag tcgaagtgta ggggcatgac 1800

atgcttgagt tgggcaccat gtgtaaggaa gaaggcgcgg gcgctagatt ctatcaagct 1860

cgtggctatt gtcgcacact cgcactagat ggccttaacg gaggcactga actaagtgga 1920

gacataagaa aaaaaataga gtagggaacg tgtccgactt gagtagcagt ggaaaaatcc 1980

aaaaaaaata tgaacaacca tcacaaataa ccaaatagta tttgtaactg gagacaatgg 2040

caacatgaag tccacaagtc acaatgtata aggttgaaat tattcttaac atgagatgtt 2100

ggcacattga aagaaagatg aacacgacga ccgagctaac acgcatgaca taaagtgctc 2160

atatctttat tacaagactg gatggcagaa taatgaacaa gtttggttag tgcctcgtgg 2220

ctgggatgta tgagatgtct ccagagcggg gatgaggtgc ttttgctttt ggtagcttgg 2280

taagaagagt acggattgca taaatgatgc tttagcggca tggctatgct agcgctcaga 2340

cgtctaatgg gtgctaacgt ttgaacactg gtggggccca ccgttgcaac atatacaaca 2400

tcactttgaa ctatcgcgac atccagttga actgttgcaa catttgaaac atgcatttga 2460

acacataaaa caactataac acacttgcac attcaaaaaa catttgttgc aacatctcga 2520

aaatcccatg caacacagaa acaatgaaca caagtacaca acattataaa tcagaaacca 2580

caacattgtt ggtcatatgg gctaggatgt acaacaacaa ggggattaat ttgaggccca 2640

aattgcaact ctagccattg gcccatcacc tttgagccag ttggagggag gagggaatag 2700

gtccccacct ttggcaccgg tcctatgact cctcgtcctc ctatttccct aggtcatatc 2760

gggctcctcg cgccaaggga tcgaagtccc atgagccccc tctgtgggct gaagtaggga 2820

ggtagtatga gcagccgagg atgatggcgt gcagacatcg ccaccacgga ggccatcgta 2880

tgctccgttg gcctcaactt tgacatactt ggtgaagagt gtggataaga atgagtgagt 2940

ggggaggaga gagcgttgtt gggagtgtgc caaacatgtg ttagatattt ctagcgatgt 3000

ctggatactt ttaaagtagc atttccattg ttaataggcc acctatcggc aacactcatt 3060

gcttggcgaa ggaaactcac attctaatat gtctatattg ttttgaacga actttttagc 3120

attttttttg cattgggtat tttttaaact taaaaatact gaactttgaa atcttggctc 3180

cactactgtg ggagaggtgc cactgtagga gacatgggtg cttaacattg gaggatagta 3240

gagcgatcga cgtgagttgc tagtgctcaa tgtctggagg ggacgagaag atactctcgt 3300

ttttttctcg aacacgtagg agagctgcgt atcattgcat taaaagatag aagaacaata 3360

caggggtcaa aaggtcctac acggaaaaga aagaaagaaa aaacacgaca ctaaaacatc 3420

tactccagag aagcaacaca tgcctcaacc taggtcatga ccataaacta agagcaagga 3480

caaaccctta gcaccagcaa gacaccacaa attacattca tcctcagcta aagacaaggc 3540

cctagcgaca ctaggcagaa cgccttcaaa aacagacacg ttgcagtgtc gccatataca 3600

ccaagctcct aaaatcacta gagttgaagc ctttttggat gcctccatta aggcgagtaa 3660

ttgaagtact gcaccaagcc tcaaaattgc tttcctcgat gcccagggac aacgcagcca 3720

gtcccctctt ctggaagatg ttgaaccaaa attgtctagc gaaaacataa gacaccaaca 3780

aatgatgaat tgtttcgtcc ttttggtcac ataaggggca aaagaccgaa tgtgaaaggc 3840

ctcttttagc caacctatca gctgtccaac accggttgtg catcacaagc caaagaaaaa 3900

acaacatttg gccggcaccc aagattgtca aatgcgctca aaaggaccaa aagaagtaga 3960

gccactgaac aacaaatcat aagccgactt agaggagtac tttccacaat tggaaaggct 4020

ccaaaaatgc ttaccatgaa tcgcatgctg cagcacataa tcatagaaaa cttcccaaag 4080

ggtcaaaaag tccaggatga cctcctagaa aacaaccccc ctgaaacaat tagtccaacg 4140

tgaattttcc aaagcctttt tgggacaagg gcgaccacac gaggggtata gtccaccagt 4200

ctcggagcca acaaccatct atcttgccaa aaagagtagt tttgccatct cccatattaa 4260

aggccataac aaataagaat atggactgca ccatagagtg ggactgcatt ggaagagcag 4320

cccaaggttt taaagagtca gttttttttg caaccacaac catctgccat gcaaggccca 4380

atttaaatac tgaagattgg aaataccaag accctcaaga tctttaggtc gagtggcagt 4440

gggccatgcc actaaacagt gacccccgtt ggcatccttg cgacctgtcc aaagaaaact 4500

acgacgaatc ttgtcaaagg ccttaatcgc ccactttgga agctcaagag ccatagcacg 4560

gtaaataatg gtggtagtga ggacagactg aactaggatg gccctgccag cctagtcatc 4620

aatgatttat tctagaggtt gtcgagaaca actatagcag gattgtccag gtggtggtgc 4680

caaatggagg aagagacgat aaggctagct tgagctttat tctaggggtt gtcgagaacg 4740

actatagtaa tatgatcaag gtggtggtgc taaacgaagg atgagatgat aaggttagct 4800

tgagttgcag gggtggtgac tagggggaag gtgttaagat tattgccatt attcaaacaa 4860

agaattacac acctggttta gaagtgctta aaaaaccaag agtgtcaaat ttgttggaac 4920

aactattgtc gcgcgtgaat taatgaacca aaaggttaca cataagtgat ggtatggcca 4980

gaacattatt tcaaatgaaa tttgattatg gcgtgataag aatgaaagta cctcatgaaa 5040

caaggatact atttgccatt gcctattgtg atgaagtaat gggtatgggg aaggcaaaag 5100

agtagtatac catattttta taattgtggg aggccggact agtatctatc tcccaaggtt 5160

taggagtctt gagggacgtg ttgttaaggg ccaaaattag tcactcttga ccccaaacca 5220

acacttgtta ttgttgttac ttggaagcct acgtcatgga gatgtaggca gactcttatg 5280

tcaatgtggg cctagagaca cagacccaac aagctgttgg agagcaccta ggtgtgtgca 5340

aagtagatgt actcacgtgg atggatgtgc tagccaaggg ttggtgtagt gcacatgcac 5400

aacaaaaata tttgttgaca ataacaacac gtaaggaaag attgccccat tgtattgcac 5460

taaggagtat ctcatgggca ggccaagaaa cacctcaaaa agttggttgt tctatacatg 5520

ccatcgagtg ctaggacaca tgtttgtggc catatgtatc ttgtggaaag tgagtctaga 5580

tggttacaca tataattcaa taatataaaa acgaggtttt ctaacctagg catgtttaaa 5640

tgcatcctca catggagtcg agcctaggac ctaaggtgct actaaggttg ttgcacccac 5700

tgggctacaa accctttcac cctataatct attgagaaca ggaaattgat taagggatgt 5760

ttccacaaga gtaaatttta ccgtcagccc ttgaacttgt tctatgttct catctcggtc 5820

ctagtacttg caaattgcac atttagctcc caaaacttat ctcgaattct tagtgttggc 5880

acttgcgttg cacgcttggc tttctaaact tgttctatgt tttcatatcg gttccagtac 5940

ttgtaaattg cgtgctcaac tctctaaact tatattactg taccatccag gtccaacccc 6000

tgcacactat gttgtcctta cccatcactc cacaagtgca tgtctcacca acgcggctac 6060

tctgtgcttc accatgcttg cctgcctacc tgcgttgcat caccgacaca atgtcatact 6120

accatgccac aatgatgcac taccatgttg tgcccttgcc gaggcgcctc actcccatgg 6180

tggtgtaggc gtacgtccac ttagtgagct tgtcgcgaca agagacgagg aaggttggaa 6240

gggcagagag ctggggtgag gagatcatga tcatgagagc atgcgcaatg ggtgagagaa 6300

aggagacaag gagtgtgtca gtattggagt tgagtgcaat taccgcgggg gcaggggtaa 6360

cgctggtggt gctgcaaggt gtaggagcga gtcgagcaag agcaaagagg gcactgaagg 6420

taggatcatg cctagacact catcttcgag aaagctaggc ttgcacgatt tggtggtgca 6480

aatgagcatg gcaatgggtg tggcaccgcg tcaacaacgc agcgtaggta agcatgggat 6540

ggtagattga tcacggtgca acacgacgca ggtgaggtgt gcacatgcat ggcaatgggg 6600

taaggacggt gtcatgcagg gcgaggggtg gactgggatg gtatagtaat acaatttttt 6660

ggagctgaat gtgcaattta ctagtacgag gatgggaacg agaactcata agtttaggga 6720

gccgagtgtg caacttacaa gtactgggat caggttgaga ccccttaagc ctgcacttat 6780

attattttaa aagacccttt cctagcattt gtttcatatg gaggataatc tcccaagttg 6840

tgtattatct attgaaatgg tgcaactttg ttgttttgag ctgggaacta atttcacttt 6900

ctaaaatctt acatatataa tattttatgt tttctttgat tgtttagtgt ttaatattta 6960

gggtggataa tacctgcttc aagctttggt acatagggac aattgcaaat agaaatggag 7020

taggactttt gattgatacg agtctcaaga acagtgtggt ggatgtgagg aggcaagaag 7080

ataggattat ctttttttct cgaacacgca gaagaactgc acatctttat attaagaaga 7140

agaagaagaa agagaggtcc aaaatagacc gctataaagc caccgaaagg tagcaataat 7200

agttacaaac taatcacctt gagcaacacg ccccctacaa caatgacatc ctctaaggcc 7260

ataagccaac caacccaaca acactagcat ggcaccaaac tcccatttag gattggattt 7320

tgcgatagct tttgacctgt tgatagctaa cacctttaag aagagagact cccatttagt 7380

gacctatttt tagataatgg aaacatagtc cgaccttggc ctcaaacgag gttacgacca 7440

attattacaa ctgaaggcaa catattgcct tgacctatca cacgacctca ccaaagaaca 7500

ccaactacta aatcaaagct acaactaagt taaacgcaac gaagaccccc atccaaaatt 7560

gtgaaagaac tgtagtagca ccaactccac atgacgacat gaagacttaa ttaattcttg 7620

gtcctccctt ctaggctgaa gctgtgtcta gaagtgacac caataagttg tcctgaaggt 7680

tacctgcaaa caagatttag gggacaattt attgaaaaca cctcattcct acttaaccaa 7740

atagcccaac aaattgtcga tgcaatggtt aatagtaagt tgcgtgatct ggacaacagg 7800

ccactcgccc aaaccccaaa caaatcatta gcatcccgag gaggtctcaa tcccgtactt 7860

atttgtaact aatgccaaat gaaagacaca tacaaacacc tgaaaaacag gtgtttcact 7920

gtctccggtc tgttacaaaa gcaacatgtc ttactaccgt tccaattcct cctagccagg 7980

ttatcctttg ttaaaattac acctcttttt aggtaccaca taaaaaaatt tattttcaaa 8040

ggaatcttaa gtttccaaag gtccttgtta tccattccca ccccattgtt gagagttgcc 8100

tgatacaaag actgaacagt aaacaaccca ttggtagtct agttccatct gaacgaatcc 8160

ctattagaat tcaaattagt atgtgctacc agcgatacca gttgaaacca ttggaccccc 8220

agatggtgaa ccaaccccct acgaaaggat atgtttaagg gcaccgtggg cagattgact 8280

ttgtcctcac aaggagagaa gataaacgac catccttgga ttgtaaagtg atacctagga 8340

agcgtgttgt ctctcaacat aagtttgtgg tggcggtctt ttgttttcag gtgtgtgtcc 8400

atagggataa acaagacaag attgcgagaa caagctggtg gaaattgaaa ggggaggagg 8460

gaaaaaagtc ttcaaggact aaaagaggac gtttggaaag aagagatgac acaaacaaca 8520

cgtgggagaa gatttttctc gaaagcgcag gagagctgcg cgaaaatata ttaagaaggg 8580

gaaaaaaagg tccaagaagg accccaagat acagataagg ccgccctatg gcggccaaaa 8640

acaagcataa atgaaaccat ccatgacaaa aacactgcta ccaaaacagc actacaacta 8700

tctagctaac aggtagacct gggatggggg cagtaagcaa ggacaacttc tttgcaccag 8760

ccataaccca aagatcaatc tccaaaccaa tgcttctaat agcagcagcc acactaggac 8820

tcttattgtc aaaaacgcag atagcaacct acgttcggaa ggtggtctca gaggtatgtg 8880

gagtaactaa ggggagtgga ggtgaagcta aagatacttg gtggttaaat gaggaagtcc 8940

aaagagctat taaggagaag aaagaatgct acaaacgcct ataccatgat aggaatgtgg 9000

acaacataga gaagtacaag gtagcaaaga agactacaaa gcaagctcta aatgtggtaa 9060

agggtagggc gtatgaggat ctttaccaac gcttgagtac gaaggaagaa gagaaggaca 9120

tatataggtt ggctaaggtt cgtgagagga agataggaga ctttaaccat gtgaagtgca 9180

ttaaggatga aacagagcac cttttggtga aggaggatgg gaccagacat agatgccaaa 9240

agtattttaa caaattgttc aatggggaca caacctttta gttgaatgac ccttttgatg 9300

acaccataga cgctttgtgc ataggatcca agaatctaag gttacagatt agggaggcat 9360

tgaaaaggat gaatacgagt aaggcgatga gccttgatgg tatcccaatt gaggcatgga 9420

gatgcctcag agacatctgt tgtatgacta accaacttgt tcaaccatat cttccggttg 9480

aacaagatgc ctgacgagtg gagagctgaa gaagtatatt ggtaccgatc aacaagaata 9540

agggagatat ttaaagttgt actaattgtt gaagtatata agtggattgc ctaccttcta 9600

acaatttaat cttttgggtt gaactggtta gtgcgtccac tctaacatgg tatcaaagtt 9660

agaggtctcc taacaattcc atgtttgtcc ctttaattgt ccttctgcac ctacacgtgt 9720

tgaactgtat aaatggattg ggttgaactg gtaagtgcgt tcactctaac atggtatcaa 9780

agcctaaggt ctcgagtttg aatcctggcg gatgctttat ttgtgcctcc gcccctttat 9840

tttcatgttt gtgccttttt ctatagatgc acgtgagtgt gggtgttgaa atgtataaat 9900

ggattgccta ccttcttcta acaacttaag tttttgggtt gaactgatta gtgcgtccac 9960

tctaacacta attacagagg aattaagttg atgagccata gtatgaagct atgggagaga 10020

gagagttatc gagtatcatc tgagaggaat aacgtagatc tctttgaacc aatttggttt 10080

aatgcttgga aggtcaacca tggaaaccat tttttaatat gacaaatgat ggagcaatat 10140

atggaacaaa agaaggatct acacatggtt tttattgact tagagaagac ttatgatgaa 10200

ataccaagta atgttatgtg gtgggctttg gacaaacata aagttccaag gaagtacgtt 10260

ggctcattac ggacgtgtac aaaaatgttg tgactagtgt tcgaataagt gatgatggca 10320

tagatgactt tccaattaga atatgattaa atcaagaatt agctttaagc ttttaccttt 10380

tgccttggtg atgaataagg tcacaaagga catacaaggg tatgtttttt tttacgaatg 10440

atgtagttct agttgatgac gggcatgagt aaatagaaaa tagagttgtg gcgggaggct 10500

ctagaatcca aaggttttag acttactaga actaaaatag aatatatgat atgtgatttc 10560

gacatacaca tgatgaaggt gatgttaatt tggaaggtca agtagtccct aggatggaca 10620

cctttaggta tttaggatca atgctacaga gtgataggga tattgatgaa aatgttaagc 10680

agggtggatg aagtggcatc aagcatctgg cgttctatat gacaagaggg taccacaaac 10740

ttgggcacct cctaagtgtc ggttcttcct ttggctggtg gctcataata ggtgctggac 10800

tgcagacaga cttgaaaaaa gaggactaaa tcatcctcct agatgccctc tttgtgacca 10860

agagcctgaa accatcaacc atcttttggt ttcttgtagc ttttccagat tatattggta 10920

catcactctt cggaaattcg ggctccacat tcttgcccct atccaagatg acacaatctt 10980

cctgcagtgg tggaaacgca ttgctgatat tgtgtcaggc atgaccaaga agggggtaaa 11040

ttgcgttgga tttgtggccc atataagaag ggatcgagtt cagaataatg atatacatga 11100

taggctaggg gtagcgccaa ttgaagaaaa gttgatgttg atccaacatc ggttgaaata 11160

gtttggatat gtccaataga gacctctaga gacgtcagtg catagtggga tcctaaggcg 11220

cgataacaat gagaagatag agaaagaccg aagttgacat gaaaagaagc tgtaaaagga 11280

gacttgaaag gatgtgatat attcaagatt tagtcttagg ccctgtttgg cacagcttat 11340

tttcaacttc ttcacaaatt taagcagagg ttctgccaaa caggcagctt ctgcagcagc 11400

ttatcagttc agctgcttct cagaatacac taaaagcagc caacaagcag aagctgcttt 11460

tcaccagctt ctcagataag ctgctttttc aacaagcacc agctgtgcca aacagggcct 11520

taataggagt gcatggaaaa caactattca tgtgcccgaa tcttgattgt gggttctatt 11580

ggatttttaa ctctagccta cccaacttgc ttgggataaa ggccctgttt ggcacagctt 11640

attttcagct tcttcacaaa tttaagcaga agttctgcca aacagctagc ttctgcagca 11700

gcttatcagt tcagctgctt ctcagaatac actaaaagca gccaacaagc agaagctgct 11760

tttcaccagc ttatcagata agctgctttt tcaacaagca gcagctgtgc caaacagggc 11820

caaaagactt tgttgttgtt gtttctttga ctgattgtta ttgttcttta agaaatttag 11880

ttatgctatc caaccttttt cagttatctc tgccgaaaat ggccagcaag catgggctta 11940

tcttgaagat aagagaaaca acatagatct tgttttgact gaggttttta tgcccggtgt 12000

atctggtatt tctctgctga gtagaatcat gagccacaat atttgcaaga acattccagt 12060

gattagtaag taactttcat ctctacagtg tagttcatat catgattaaa atgttgcttt 12120

catcatttat ttagtcccaa tgcttttagc tgctacactg atgttgtttg tgtgcagtga 12180

tgtcttcgag tgatgctatg agtacagtct ttaaatgttt gtcaaaaggt gctgttgact 12240

ttttagtcaa gcctatacgt aagaatgaac ttaagaacct ttggcagcat gtgtggagac 12300

aacggtgtca cagtgtaagt tgttcatggt ctggtttgaa tattgtaagt tctttgacgt 12360

tattcatgtt tctctgggta aaattgtata attactctaa caagagttta cttatttggg 12420

aaacaagaag acacctattg aggtatggtc tggtaaaaca taaggctgtc cctttagacg 12480

tgatgagctc tcccgctccc agcccctctc cctcccccag cccctctccc tctccccctc 12540

acacctccac cagcctctcg ccggaatcca ctccggaccc gtcgaccaac tcttcggccc 12600

ccaccccctc ggagattgcc cccagtcagg ccagtcgccc gatcccccag gaggcaggac 12660

gttctaagtc ccagagatgg tgtgatgacg ttattgatgg tgactcatcg ggaggatcct 12720

cctctgctta tcattcctat aaagaagctc tccttcaacc ttctggtcat ggtggtaacg 12780

cccctgctcc ccctattccc cacttgaaga cgaaagctgg tgaccagtca gaggtgcaag 12840

ggtcttctgc tgaaagtcga cgtcgaaagg aggatggctg gcaggtggtt gagcatagga 12900

ggcgccacct tcctcgtcga ggatccatag tggatctccg agggaagtgc tacaagtgcc 12960

tttctacctc ccacctggta gcttcctgcc gacgtcctac gaggtgctat cgctgcttca 13020

agtttggcca tcaagcggca aggtgcccct tgccggaagc tgggcgaaag acagtgtggc 13080

agcggctggg ctctggtgga gatcgtactc cggtgtggct gcgtttgtct ggagtggaca 13140

attctggccc aacggtgatg gatcgtccag tcattcagcg caaggcccca gtctggcgaa 13200

ggctttcccc tccagtgaac tccccattag tggaaaaaga agcacagggt ggcatgcttc 13260

tgcctgttgg acgtatgaag aagaagagaa gacgttctaa acgcgggaaa ggcagctcgg 13320

gactgcagtc gggcccaagt cagccgcatc ccaatccccc tgttgctgtc cagagcgcct 13380

tgccgcggaa accttcctgc gtattggagt tttccactga tttggcaaga aaggaagctg 13440

ctcttcggag agccttgttt gtttcggtgg ttggcaccag gccgatggtt cgcggggatg 13500

aggtgattga ggaattggcg cgaagtttca gcatcaaccc tgatgatatg tctatccagc 13560

aggccacgcc ggaagatttc ctactattcc tgcctgatga acacacagcg acccaaattc 13620

ttaacgaagg acgacctttc agaggccctg gattttctct caattttaaa agatggacaa 13680

gattctctca tgcctcgacg acgtctatgg cggcactggt tgatattgag attcgaggaa 13740

ttccagctca tgcttgggat atctccacgg caaaaatgtt actacaggat tcgtgctggg 13800

ttttggaatt gttaagggat tcaggaacag catttctgct acgcgcctgg tgttttgacc 13860

caaaatatct gcattcagaa atggaacttc atatcgtcga gccgggcagt gatgagcaga 13920

agaaacgatg tttgacatac aacatttcca tgacagtgtc atcggtgccc tttcagatga 13980

ccaatagcac tgaaactcta tccccttcct ctgacgatgg tcagtttgat gaaggaaatg 14040

acagtgatcc agctgactcc cagcagcgcc gccttccacc tcagccacct agggaatctg 14100

ttcaccttcg gataggcgcc cagtttgtgt ctggaaggag tagggcgctg tgttcggttg 14160

aggaagctga aaggatgggc ttaggcgcct cttcgggact taatgcacaa cattcaatgc 14220

ggccccttga agattgcccg acctggggag atgagatggg aatgcagctg gtctgtggcg 14280

cctcgggtcc ttccaggcag atatgtggcg ccttgggtcc ttccaggcgg atatgcggcc 14340

agccgtccat tgatgcttgc gttgcccaat taaataacgc acagctcgat gctcgggccg 14400

gctccttcat atcggtggag agatcccagg agtcgccttc cccttcatgg aagcagtgta 14460

gacagttggc taatgaggca cgcgaccttg gggtccgagc tgcctccttt aatgatgaag 14520

aaagaaccaa ggtgccacta gctgttgggt cgctgaatca gttgaggtca gcctcgcccc 14580

agtaccattt agctgctggg ccgctggagg gggaccggac tgattcgccc caagctactg 14640

ggcctttggg atttacgggt gaagcacgcc aggatgatct tgtggctgaa ccggtggatt 14700

gggctacgac ggacctcacc catcaaatgt gcatttctga gactgagagg acgaccttga 14760

ctctgggcga tcatgtggct gcggacagcg gaaggaagtc ctcaaccggg aatttgctac 14820

accaatccat tcactgcact actggcattg aacagccagg gatgctacat attaggggcc 14880

ttcaatcatc caaaaagctg atggtatact cacgaaaaag gttcaacagt agggacaagt 14940

ctggaaatat gaatgaagaa gggagtgggc caatcgcttc tgtactgagg attgatactg 15000

atgaaaatac agtgtccacg gtgcgagagg tcagcctagc tgttactact gatggtagtc 15060

agtcaacttc gcaagagacc caaacaggca gtacgcagct caagaacaaa caattcctta 15120

gcaagctctc caaaaagatt tcaggcctcc ttgcaacacc agaagatccc ccttttatga 15180

cgtctaaaaa tgcccaagtt gcagaaatta tcccacgccg cagccgtcga attgctggcg 15240

tgggagttga atttgacaac atggatttat cctcaagaac cacaaagaaa gttatgcagg 15300

ccttgaaagt aattggggat cctggggcag tcacccagca agccaaagaa gactatctga 15360

aagtattctc cactgaactt cctatttcac atattgaagc gctggcgtcc ttttttggat 15420

gggtggtacc agatgatctc aaggtgggca gcattagtgt tggcattagt gcctgttgag 15480

ttgtcctgta gaggtcgctg atgtaagagg tttgtaatgg atccaaaaaa aatcttgagc 15540

tggaatgtaa ggggtctcaa ttctacatct aggcaatgtt ctgttagaac ttgcattgat 15600

gcttgtggcg cagacgttat ctgtcttcag gaaacaaaaa tgcaggaaat taacagagga 15660

aaggtgctgg ctattttggg aacagatttt gatcagtatg taatgctgcc gtccgagggt 15720

gctagggggg gaattctgat ttcctggaaa aagcatataa aggtgacagg gctgaacaga 15780

gtggattctt atagtatctc cttgcaattc cagagaaata atgggcagtc ttggtggctg 15840

acttgtgtct atggacctca aagtaattct gaaaagatcc tttttcttca agagttaagg 15900

catttcagaa gcatctgctc tgagccttgg gtaatagtgg gagactttaa tctcatctac 15960

aaggttgaag acaagaataa tgataatatt aatagatcta tgatgggtcg cttcagaaaa 16020

tttatcaatg atgttggtct gattgatact cccctaattg gtcgcaaata tacctggtcc 16080

aatcatcaat cttcaccaac actggtccga ctggatagag ttctctgcac tactgattgg 16140

gaagcgttgt tccctaacca tctccttcaa agtgctgctt cagaggactc agatcattgc 16200

ccactgattc ttggacttaa cagtatgtgt tctggcaagc gtcgatttca tttcgaatcc 16260

ttttggccaa aattggaagg gttccaagag gtagttctgg aagcctggaa ttcggttaat 16320

gaaagaagtt gccctttcct gacattcgaa ctgaaattga aatcaactgc caaggctctg 16380

cagagatgga gtgctaagaa ggtaggccat gtgtcctccc agttggcact tgctagagaa 16440

attcttcatc agctggagat tgcccaagac tctcggctcc tgtctccttt ggaggtttgg 16500

ctcttgagag ggcttaaaaa acactcctta gcccttgcct ccctcaaacg cactattgcc 16560

cgcctacggt ccagaatcag ttggataaag gagggcgatg ccaactctaa gcttttccat 16620

atgcatgcta ggcacaggca aaggaagaac tttgttgcca agattgtgtc agggatgaac 16680

actttcactg atcacggcga gaaggctaga gcagtagatg agtttttatt tcaaactttt 16740

gggctgcaag gcggacagat ccttgactgt tgatttggat tacctgggtc taccttctca 16800

tgacctgact gatttagatg cgcagattga tgaaaatgag gttctggaat ccataatgca 16860

actaccttct gataaggctc cggggcctga tggttacaca ggcagatttt ataaagtttg 16920

ttggccaatt atcaagacgg atgtgatggc tgttattgct gcaatttggt gcagaaaatt 16980

ccagcagttt ggaagactta acactgcatt tgttactctt atccctaaga aggagggggc 17040

tgaggaagta aaagactttc gacctatcag tctggtacac agcattgcta aacttgtcac 17100

caagttgatg gccaacagat tgtcccaacg actgcatgat cttgtttctc ttaggcagag 17160

cgccttcatc aagggccgtt tcatccaaga caacttcatg ctggttcagc agactgccag 17220

attgcttcac cagcaaggcc aaccgagggt tcttttgaag cttgacatca ctaaagcctt 17280

cgattctgtt tcttggcctt ttttgcttga agtacttcag agattgggtt tcgggcagat 17340

ttggagggat atcttaagtg gccttttagc aacttcctcc acacaggttt tactcaatgg 17400

agttccgggg aacaccatcc cccacaagag agggctgcgg caaggggatc ccctctcccc 17460

tatgcttttt attttagtga tggacatcct tggttgcatt gtggccaaag ctgatgagga 17520

ggggcagctt gagcctctct cttccaaacc actgcaccac agaatttcgc tatatgctga 17580

tgacgcggtt ctttttttcc acccaaggga gagtgaaatt catactgtcc taggtattct 17640

gaatttgttt ggagaagctt caggattaaa aactaatatt cagaaaagca gtgtgtatcc 17700

aatccgttgt gggcaggatg agctggacat cctcaatgat tggcttcctt gtgaactttc 17760

gtctttccca tgcaaatatc ttggtctacc tttgtcctta aaaaagctgt ccaataatca 17820

gctgcagccc attattgaca aagtggctga tcaactttct gggtggaaag cggacctcat 17880

gacacgggca ggtagaagag tgcaggttca gtacgtgatg actggcatgc ttatatatct 17940

agtcatggca gttgacattc ccacaggctc gctcaaagct atcgacaaga tcagaaaagg 18000

atttctctgg cgtgggagga aagaggtcag aggaggacat tgtttagtgg cttggggaag 18060

agtttgtaga cctcttgaaa ttggtggact tgggatttct agtcttaaag aactaggatg 18120

ggccttaaga atgagatggt tgtggctgcg caaaacggat ccggataagc cttggtcctc 18180

attgcccatg cagatatcca acaaaataaa ggcctttttt tgcaccgccc tgctcactga 18240

aattggaaat gggtcctcaa ccttattttg gctggatcgt tgggtccacg gaaagaatat 18300

tgaagatatt gctcctcgac tgcttgcagc ggtaccaaag agaattagga acagcagaac 18360

tgtgcttgag gccttgacag acaggagatg gctaactgat attaaagggg ccttgacagt 18420

aggtgtcttt gccgatctcc tagatctctg ggatgaactt caaagtgtcc agcttcattc 18480

agacagggag gacaaacata ttttcaggtt cgctaatgat ggaatttatt cagcgaaagc 18540

agcttacaat ggcctcttca tcgggtcgac catggctaag tattgggaac taatttggaa 18600

aacctggtcc cctcccaagt gtaaattctt tttatggctt gctgatcttg gaaggtgctg 18660

gacagcagat cgtttgcaga agagaggtct tagccatcct gacaaatgtg tcctgtgtga 18720

ccaagagcaa gaaaccatag atcacatttt ggtggggtgt gtttttgcta ggagtttttg 18780

gtttcagctg ctgggacagg tcaacttatc tgcctttact cctgtgatgg gagaaactaa 18840

aatgatggag tggtggagca gaagcagtga gcagttacag gggattgcta aaaagggatt 18900

caattctttg attacccttg ggctttggac tctctggaac cacagaaatg gatgtgtttt 18960

tgatagagct acacctagca tggagggtgc tatcagaaga gctgaagaag aaattattat 19020

ttggaatttt gcgggggcca aacatcttgc ccttattaca gctcccattc caggtgtcat 19080

gtagggatct aggagtgagt tcagtagtta acctcactag ggaagggtgt tggttggtta 19140

agtttagatt gtgggggtag gttggtctag tttgctttct ttcttctcct ttttcttttt 19200

ctttttcttt atatttcttt ttctgttgtg tttgtaattg gtctaatttt tagacccttc 19260

ttcttaatac aatgatacgc agctctcctg cgtgttcgaa aaaaaaaaaa gtttacttat 19320

ttggaactca gtccagtggt agtggaagtg aaagtggcat tcagacgcag aagtgtacca 19380

agtcaaaagg tgccaatgaa tccggtaaca acagtggtag caacgacgat gaagctggca 19440

tgggacttaa tgcaagggat gacagtgata atggcagtgg cactcaagta tgggttttga 19500

tacttttttt tacttctcta aagcttatta ctggctgtta tcatgttgtt caaggtatga 19560

acgtagtaac acttagaata caagaaacat tttggcgcaa ctgcctataa tcaccaaatg 19620

gaatgtatta gaggtcagtt cacatagcag ctaaatttaa ttgtcattta taaccacaga 19680

attagacagt gtattgctgt cactactaaa tgttaatttc ttaggataac tatttctata 19740

tatatgctag aggtaccaca ttcctggttg aaacaaatga gtaaattact ctggatctta 19800

atttaattct aaaaaattgc acatgaacta gagaatgcct gtgatgtggt tttatttcac 19860

ctaacacact taagtccatc ttgcctctgt tctattttta aacttgacct gcggggggta 19920

agacagcccc cgggcattgt attaagaaca agaccttctc acacaggtcg agaaaacccc 19980

cgaaccccta ccccacccat acacagcggc atcgaagccc atgtgagaac gaccacgacc 20040

ggggctgagc cttgaacctg tgctttagcg tgggacagac gaggggattt ttttaaccac 20100

agcttgaaat tcgctcccac ggagagtcaa acccaggacc tgaggagtgc tactcagacc 20160

acctaaccaa ctcagctaga ggccctttcg ctctgttcta tttttaggct aaaagaaaaa 20220

tgccgactgt attatcttac tgtttcttga acacacatga gagatatgta tcttatatca 20280

ccccttgtat ttttatacaa ttatatgtta gtttagttgc atccagactt ttgcttaaat 20340

agtagcaagg gttcactgta accagttctt tgttcagaat ccatttagaa atatttggtt 20400

gtggaaaagc ttgaggtgaa ctgtttggtt cagaaacaaa ttattgtttc atatccacag 20460

tccatgagtt ttgacgtcaa aagctcatac tgatagaaaa acagagaccc actggatcag 20520

cctaagtccg acacaacctc aatgatgata agggccctaa ttctaatctg aagggttgag 20580

ggggaaggga aaactagcat acttactgtc caagagtaat ggcggcattg ggcatcgccg 20640

tcgtgcccaa ctgcccatcg ctggtgctcc acctccctgt tgtcctcctc cattgtcctg 20700

ccccatgatt agctggagaa agtgggggct cctcggatcc cggccctgtg ggggtggctg 20760

ggctctatgg acttcatcga ttgtggctct tccggtcgct gagaacgccg ggaattgtgg 20820

tggctagggt tttcgggcgc tagaatgtgt tggcgtctct gctggcctag gcctcctatt 20880

tatgttgtgt tctcgtgacc cggggggggg gggggggcaa ccataggttc ggtttgtgcc 20940

cccgatcagg gcgtgcgaca ccatgcactt ttggggagtt tggtgcttat tcaacttggt 21000

tctgttgctg agtcagtgaa catcctaaca aatacacgta agaaccaagt ttgaaatttt 21060

cacgtttgcc actgatgttc tcaatgccat ttgtccacat gagaacattt tgtaacttag 21120

gagtgatatt tactgtatct tttaaacata aaaaatgttt ttaaacttca gatatgttaa 21180

gttaatttta cttattaaac ttgtcgtttt caataatctt ttgctccttt tgtaaatttc 21240

ttttgaacac cgcttgcatt ttaataaaat gtagtttctc caatgtgttg tgaaaatgca 21300

tatgttgtgt tgaatttgtg gccatacaag aagggatcaa gtttgaaacg atgatatacg 21360

tgatagacta agggtagcac caattgaaga aaagttgttc aacaccgatt gagatggttt 21420

ggacatgttc aatggagatc tctagaggca tcagtttttt ttcttgaacc atgcaggaga 21480

attgtgtcat tttatttaag gaggaaaaat aattacatcg ggtaggcaaa aatgccccac 21540

ccgaacgcca cagacaccca acatgggact actcaaagag gagtcacgac ccggaccaat 21600

agccctaccc agctaagacc tgcgaccttc tattttgctc taggaggttg gcagctccgg 21660

ccctacacca aagcaaacct tcgtcatcta ttaactggag aacccgccga acatctggct 21720

gactggcctc aaacacaaaa gagttcctga atttccaaat ctcccaagcc actaaaatga 21780

acaaaaagtg ttgaaccctt tccgcacttt cttatgcacc atcttgatgg agcgtgccac 21840

taggaaaaga aatgagtatc agctgtagaa ggcgtaagag gggcaaggtt tagggagtga 21900

aggatcatgg tccagatttg tcgtgagaac acacagccca cgaagatgtg ttggatggtc 21960

tcgtccattg ttttcaagtc gaacgacttc ctggtcattc tggctgaccg acttgggcta 22020

ttaatcgtga ttaatcacga ttgatcggac gacttggacg attaatcgtg atcggtccaa 22080

actgacttgg atgattaatc acgattaatt ggacgacttg aaaacagtgg tcttgtttgc 22140

ctgatcacag aaggtatagg ccatggggta tgggagacct cacttggcaa ggcgatccac 22200

cgtccaacaa cggtgatttt ttgcaagcca aaggaagaac atgtacttca agggagccca 22260

gcctttccag atattcttcc aaccattaaa actgacagag tccaaaaaga acatggagta 22320

ggctgacttg ctgctataaa ttccggattg agtgaacttc cagtggtgtt gatcagacac 22380

gccctgctgg agaggcatgt catcaaccaa gtcccaaatc actggatatt caatcagcac 22440

ttgcacagat agagcacctc tgatgtcagc aacccaggtt cttttttttc tcgaacacgc 22500

aggagatttg cgtatcattc cattaataga tagaagaagg tacaacacac cacacacgac 22560

acacacctga cacgacacac acacacatga cacaacaaga cacaagacgc aacacagcaa 22620

ccacacacaa ccactctgaa caagccacta gactatcccc ctggtcaagc ataaacataa 22680

gagttgaaag caggttcaac cctttcgccc cggcactaca ccacatcctg agctcatcgc 22740

cagcgagggt gagagcactc gccaggctcg gcgatctccc attaaacaca cagtcgttgc 22800

gatgacgcca aaggctccaa gctccaagaa taactagaga attgaagccg gattggagat 22860

cacggggaat cttctccaca gccctgctcc accagtcatc aaggctgcaa ttagcattat 22920

ctggtgaaag gttaggcaac ccagacctgc ttaacaagga ataccagaac tgtcgtgcaa 22980

agacgcaaga gatgagaaga tgttgtattg tttcctcttc ttggtcgcaa agggggcaca 23040

cactggggtg gggtagcccc cttctagcaa gacggtctgc ggtccagcat ctgttatgca 23100

tgacaagcca aaggaagaaa ctgcactttg aaggtgccca agattcccag atgcgctcat 23160

atgcgcccaa agaagtggaa ccaagaaata acatatcata agccgacttt gaagagtatt 23220

ggcctgtcgg ggagaatttc cagatatgct tgtctggcac tccaacatta atggagtaat 23280

ctgcaatgag ttcattaaga gttaaaaaat cagagaggac atcccaagtg atggtgcctc 23340

tcaggtcccg aatccaagcc atctccacga caccttcagc caccgttctc ctattggtaa 23400

tcctggtcgg gacgagggcg aaaatatgag gagttagatc ctcaatcctc aaccccatca 23460

accatctgtc cctccaaaat aaggtgttac gaccatcacc aatttctgta atcaaagctg 23520

tagagaggat agatttcaga gtgctggaac tctggagagg taaatcagcc caagacttcg 23580

agtgatctgt tctttccaac cacagccatc taacccgaag ggcccaattc agcttttgta 23640

aatctgagat gccaagccca ccaagctcct ttgggcgtgt cactttgccc caagcgacta 23700

agcagtggcc accgagcaca tccttacgac ctttccaaag aaagccacgt ctaatcttgt 23760

caatggcttt gatagcccac ttaggtaaat ccaatgccat ggcgtgatat ataactgttg 23820

ccgtgagcac atgctgcact tggatggctc tgccagctcg agtcatcagg tcggccttcc 23880

aattaggtaa gaggtccgca agcctatcga ttagtggttg cagctgctcc ttgcttagct 23940

tcctgaggga aagaggcaac ccaggttcta ttgtggagga gggcttgagc cacagtcctg 24000

ttcttgactg ccctctttgg gactgcagag agtagagatg gcgccaaatc agctacgcac 24060

tttccatcga accacctatc tgtccagaat tttgttgaca ttccatctcc aacagtagtg 24120

cgaacaacca tatcaaacaa gacccttgcc tttgacagga caaaaacagg aagcccgccc 24180

aaagtctatt gagaaggttg aagttgatgt gagagactaa aataagattt gaaaagatgg 24240

aatataccca aagatttagc cttgaatagg agcgaataga aaacaactat ccacatgctt 24300

gaatcttgtg gtttatgtta ggttttaact ctagcctgcc tcaacttact tgagactaaa 24360

atgctttgtt gttgttgttg ttgtttctcc aatgtactac taagttatta ctgatatgtc 24420

taaaaggcct ctaacttagt tgattaggtg gtctaagtag cactcctcag gcccggagtt 24480

tgactctccg tgggagcaaa tttcatgacg tggttaaaaa aatcacctcg tcttcccacg 24540

ccaaagcata gttctaaggc ctgcccagtc gcagtcgttt tcaatgggct accgtgccgc 24600

tgtgcatggt ggggtaggga ttcgggggtt ttgtagacct acgtgataat gtcttattaa 24660

tatattgccg tggggcggtc ttatctccgc aggtagagtt ttttctactg ataaacgaca 24720

ttagggaatg ggtactgtat ttacaaaatg aactaagtgc aacatgagat atttggtttg 24780

catattatat cctaccatca cacaactaga cataacctga tatggaaacc aaataaaaga 24840

ttttttcttg cagataataa ctaggtggac taacctttaa taattgtata ccattaccac 24900

ataagcagga aaaaattaca cagtgaagac atagtttggt tatgtgtgaa agacgataac 24960

attatgatat gtggattact tatgaggtcc tcaaaagaac ttatgaggca aaagaagtgc 25020

atattttcaa atttgtagtc ttgaaaacgt gaagctgtat ggtttcctgg caaccttgca 25080

agcacatgta cgtattagga taaactatga tgttttcatg ttgacaggat ttaaacgatc 25140

ttgttggcgc ctttttgggc caaacactaa gggtccctgc ctccctggat cccgctcgcg 25200

gggaacgaga ccttggagaa cctctctgcg agatggtaga taaaatataa gacaaataac 25260

actacctgca atacgctcat gaactttacc tctatgatgg caccttctta ggaccaacta 25320

gcaacgaacc ctagggcctt catgtgaagt gcaagacctc gaattcggct ctgcgaggtg 25380

ccggacgatc tgcggtaggt tgtcggacca tcaacgatag ggcagcggac cgtccgtgac 25440

gacatagaga gctcttgggt gaacctggat ctcgcccctg ggagggatct cgtcaggcct 25500

agggtcttgt tagggtcggc aggccaccca cgacgtctct agatgacgta gagtcgaata 25560

caggtgaaga ttgatatgtg gaaactacga ctaaagctgc actagatcta ctcctaagac 25620

atgaatgata aataaggtaa tgtaaataga ttgattcgat tggaatgtgt tcggtgggtt 25680

ttcaatcggt tgtacccctt tatgtttata gggggaggtc tggacccgtt cctaggcaag 25740

atccaatgga ttcccacggt taggttagga taaccatgca tgggataagt actctcgctc 25800

gagttagtct gatcgtggac tgtccggccg tgcccatgtg ccaaatctgg tgctcaacac 25860

ataccccctg ccttttgatg gagcttggtg aaccaaaagc acctggtact accagaatct 25920

ttcgagaaaa catagattct gcaacttgcc ttgttcccga aatatgaact ctagcccgat 25980

atatgtcatt ggctcttgtg atcagataaa actatttgat agaactctaa tgcacagaga 26040

ccgtttaggt ttgcatcctc ttcaaccata tctacttcat caatatgtca ataggcaaaa 26100

acatgtggtg cgccccagcc taaataagca aacagattgg gcaagtaata cagattcatt 26160

gtcgtatcac ctcacacttg agtatgacaa cacattggcg atgaatagac cggaaagtac 26220

cacgacatcc ctggaggtgg atgagcaggt gaccttggct gcaccacttt tcgggccggt 26280

ttagtcagcc tttgcctttg cacagatcgc ctctttgact gttttattgg ccggtggtgc 26340

aaaacggcct tctccttcat atatttggca agaagttgat caaaagtagg gtcagcactg 26400

ctgagccaac caagtgtctt gggggtgttt tgcttcccag tgttgaagtg tataggtgga 26460

cgaactacct tctcctatca gcttaagctt gggttgaact gattagtgcg tccactctaa 26520

catggtatca tagccagagg tctcgagttc gaatcctggc agaggcttta tttgtgcctc 26580

cacccattta tttccatgtt tgtgtctttc tctttgtctg cgtttgcacc tttctctctg 26640

gctgcacgtg agtgagggtg ttgaaatgta taagtggata aactaccttc tcccatcagc 26700

ttaagctttt aggttgaact ggttagtgca tccactctaa cacccagtac atatttcagg 26760

atgttgtagt ctgacggtct gaggtcgccg ccgcttctag ttgtccgcgg attgtccgac 26820

ctttatggtt ggactattcg catctgttct ggactatccg gccttagtgt ccagaccttc 26880

cggcataccc aggataggtg accgagatcg ggtatccgat cgtgcttccc cccggtgctt 26940

tcagacttat tagcctttct tttgtccgga gcctttcgac taaccactcc acgtgataga 27000

tttggcgtgc gagggtcacc aataataata ttttcgcctt tgcctttatc ggccgcacac 27060

ggccgaacca agactttctt gtctgtcggg ctctaatgtg ttgacaggga catgttgcct 27120

gtcaattttt atttcttgaa atctcaatcg accttcattt atagctgatt gtatttaccg 27180

acggaagaca ttacaatcat tggtatcatg aaaaaaggag ccaacaatgc acacgccttt 27240

tcaatttcta tatcggagga attgtgtgag acaatttaat gttaccattc ttaagcaact 27300

catcaaaaaa attatcacac ttagcaacat taaatgtaaa cttaacttct ccccaccgat 27360

tcttttgaat cggctgtagg gaagagcaag cagaaagttt agccttagca agacaaataa 27420

gcttagcggg atgtacctct tttggctcgt catctgagct actactgcca cgacgctcat 27480

catgacaaga ctgttctttg ctttggcttt cacaagtcaa agcccgctgg tgcaatttag 27540

ttagcgaaaa aactagatcc tgtctaattt ttctttcatg tatggacata acacattgaa 27600

agccaaccct gctagttgct tgtccacgac ctggattcga gaacactggt ttctagtatt 27660

ccagaatctc tagatataat ctttaataga ttcatcgtcc tctttctgga tcgatgccag 27720

atcaactaaa tctaactcgt aatcactaga gaagaagtgt tcatgaaact tatgctataa 27780

atcatcccaa gaattaatcg agttaggagg taacacatca taccaagcga acacagtgcc 27840

agtaagggat agtgagaata ggcgaacacg aaaagcttct gtgtcgtcca attcccccaa 27900

ttgtgctaga aattggccta cgtgttcgtg agtgccttta ttgccttcgc ttgagaattt 27960

ggagaaatct gacatactag tccccggagg atatggaacg gtgtcccatt gagggtcgta 28020

tggcctctga tgggactatc ctaggccgga tacactgaca cctaatttat cccgaaattg 28080

ttcgactatt tcttctcgaa cttcatccat agcgatcgat ggtaacccat cggtcctcgg 28140

gacatggggc tcatgtggtc ggttatcctg atgcgggcct tctgcactag gacgatcaaa 28200

ggtgacgttc ggtataggcc cctgaggcat acctgtttca taccccattg tcgtttggta 28260

tgaggtgaac ctttctggct ccctagggat cgaaaaatat tcaacctcat gcatgggtga 28320

tggcatgcta tgactacgat gtgctgtagg tggggtgtag tgtcgctgag gtgagtagga 28380

aaattgttcc tgacgtgcaa cactctgtcc tgtgtagatg tactcggacc gtccgacctg 28440

ggtggccaga cgatccgcga agggactaga cgacccagta ctgtacccgg actgtccagt 28500

tgtgctggca tctcatgcga gccatgtggc gggctcggtg tggctctgaa ctgcccgacg 28560

taaggtgctg aaaggtctgc tatgaaatcg gactgtccga ggttatatct ggactgaccg 28620

acagtatttg gcaccgcatg tgtgccacat ggcgggctcg cgttgcactg gacagtccga 28680

catatggagc cggacagttc gtgcgatggc cagcggagtc agaaagttca ccgcctaata 28740

gatctgactt aatagatccg atctgatgga tctgatctaa taaatctaat ctcagtcatc 28800

cgcagcggag tcaccaaaaa gtgtgttggc gcctttttgg gccaaacact aatgatccct 28860

agaccccgct cgaggggaac gagaccttgg ggaacctctc tacgagatgg tagataaaat 28920

ataagacaaa taacactacc tataatatgg ttttgaactt cacctctatg ctagcacctt 28980

cttagggtca actagcaatg aaccctaggt ccttcacgtg atgtgcagga ccccaagttt 29040

ggctctgcga ggtgccgatt ggtccgcgat aggttgctgg accgttcgcg gtaggcacca 29100

gaccgtccgc gatagggcgg tagaccatcc acgatggtgc aaagagctct tggatctcgc 29160

cccccgggag ggaccttgtc agggaaagat tctaaggtcg tcatggggcc gacaggccac 29220

ccaagacacc tctagacgat gtggagtcga atagaggtga agattgatat gtggaaacta 29280

cgactaaagc tacgctagat ctactcttaa gaaatgaatg ataaataagg taatgtaaat 29340

aaattggttc gattggattg tgttcggggg ttatcaatcg gtcgtaccct ttatatttat 29400

agggggggtc ttcacccatt cgtaggcgag atccaacaga ttcccacgaa taggttaaga 29460

taaccatgct tgggataagt actttttttc tctccgaaaa atgcaggaga gttgcgtatc 29520

attatattaa gaagaagaaa aggtgaggta aaaagagaac ctcatacaac tccacgcaaa 29580

acccacacac actataatag atgcagattt acaaaaatag acagctagac ccgtgacctt 29640

gaagacccca aaccagaggt caccgattgg tgtgctggag cgctaggatg ttagataatc 29700

cacgggctcg agccctacac caaagatgag actcctcctt gacccaattg attaccaagt 29760

tagtacaagg ctggacaccg ttgaaaacac agcggttgcg gtgattccaa atggcccaag 29820

ctgcaagaat gatcagggaa ttcagaccct tgtgcagccc cttgtatgct ggtccaacag 29880

tagaagagga aactccttct caccaatcct taaaagaaga attatcatgg ccaggagcaa 29940

ggtgttgcag cccaacagat ctcataatct gaaaccaaac ttcccgagag aaaacacagg 30000

agactaacaa atggttcaag gtctcctcct gatctcatag caaacagttt tgggggtgag 30060

gtaacccttt ttttgccaga cgatcagctg tccagcagcg accgtgagcc acaagccgca 30120

tgaagaactt gcattttcca ggtgcccaac atttccagat cctcttccaa gggataagta 30180

ctctcgcccg agttaatctg atcgcggact gtccgagtta tctcgcggac cgtttggggc 30240

acatggccgc accgtccgac cgtgcttagg tgccaaatat ggtgctcaac ggatctagta 30300

gggttgggtg gtgttcagag aatgtaagta catttttatg attatcatga ccattagatc 30360

agcttgcata tgtctgcatg ataacttgtc cagaaagtat tgtggtttat ttgttggtga 30420

tggctaccgt gtcaacgcaa cattggtcca tacctttgat ttgaaatatt tgggtgctgt 30480

taacaaagtg tatacctcca agccaaggta gtaaatgttg gacctgaagt gttaaaacat 30540

ggctcagctg gttgggtggt aggtgtggtc gtgcacccaa tcaccgaagg tacaaggcct 30600

cggcttgaat ttgggtgcct atttctctat tttcattttt aattaaaaaa acgtctagtt 30660

tcttctaggt tggatcttga acatatgaat ttcatgattt aaatcatatt atttttcatt 30720

aacgggttat gaacaaggtg atctattctt ataattcgag atgaactgaa ttttgttttt 30780

ctttctttta ctttagttac atagtcatgt atcaattatt tttgaactaa gccataccta 30840

ttttctgtct ctggtttttc tgtggtaagg tatcttactg acaggtgggt ctaagcccat 30900

gtagcactga aagatctcag tggtgaagtt gctatatata tcggaaatta aagcaatgtt 30960

ccccagtgga gttgctatgt atatatcaaa attaaagcat gggcacatta taacttgttt 31020

ccttgttaat atttcataag gcacagaact catggactaa gcttgctgtg gagatcgaca 31080

gtccacaagc tatgtctctg gaccagttag ccgatccagc caatagcacc tgtgcacaag 31140

taatccactc aaagtcagag atatgtagcc acagatggct accaggtagc agcaacagaa 31200

actgcaagaa gcaaaaatac actaatggta tgccatgctc aactgatgca ctatgcaatt 31260

ttacaaaata aataaacttg ctggccattt tatctgttat gtttgatgct tgatttcaga 31320

tgactttaag ggaaaagact tggagataga tggtcctgga aatctatata tggatcacca 31380

atcttcccca aatgagagac ccatcaaagc agcagatcat gaaaacaatt ccaaggagtc 31440

aatgatggga aatttagggg atgcaactgt tcgagctgct gacctaattg gttcaatggc 31500

caaaaacatg gatgcccaac aggcagcaag agctgcagat acccctaatt tctcttctga 31560

agtgccagaa gggaaaggta agaatggtat gtcatgctca actgatgcac tatgcaattt 31620

tacaaaataa ttaaacttgc tggccatttt atctattaag tttgatggtt gatttcagat 31680

gactttaagg gaaaggactt ggagataggt ggccctagaa atttatatat ggatcaccaa 31740

tcttccccaa atgagagacc catcaaagca gcagatcatg aaaacaactc caaggagtca 31800

atgatagaaa atttagagga gccaactgtt cgagctgctg acctaattgg ttcaatggcc 31860

aaaaacatgg atacccaaca ggcagcaaga gctgcagaag atacccctaa tttctcttcc 31920

aaagtgccag aagggaaagg taagaatgac cagcatgata attatgtttt gccatcactt 31980

gagctgagtt tgaagaggtc gagatcatgt ggagatggtg ccaacgatac agtcaatgac 32040

gatgaacagc ggaatagtgc attaaggcga tcgaatctct cggcttttac aaggtgcaaa 32100

acataatatc agtgtcatta gtgagttatg aaagcaacag tatgttgggt actaacagtt 32160

accactttta ttgcaaggta ccatacatct gcggcttcca atcaaggtgg gacaggattg 32220

gtaggtagct gttcgccgca tgataacagc tcggaggctg tgaaaacgga ttctacttac 32280

aacatgaagt caaattcaga tgctgccgca ataaaacaag gctccaacgg cagtagcaat 32340

aacaatgaca tgggttccac tacaaaggat gttgtgacaa aacccagcac aaataacgag 32400

agggtgatgt tgccctctgc tattaaggct aatggataca cgtcaacatt ccatcctgtg 32460

cagcagtgga tggttccaga taatgcgaca gcagggaaag cgaaggctga tgaagtggcc 32520

aacaatgcag gcagaaatag tcatcctggt gacgtgcaga gcaacctgac gcaacaacat 32580

cgtccaaatg ctggacggac agatacagag atacgggcta tcgataaaag tggacctgga 32640

ggtggcagtg ggagtggcaa tgacacatat gtcagacggc ttgctgcgag catgacacca 32700

cgacaagaac agctaaagaa atatagagag aaaaagaaag atcggaactt tgggaaaaag 32760

gtagcctgtt ttcaattgca tattaatatt tattctttat ttgttttcaa ttgcatatgt 32820

ttaagaagag agagcattgg atagaaggac tggtttcttt ctgatggctg tctgccattt 32880

ccaggtgcgg taccagagca gaaagaggct agccgaccag cggccaaggg ttcgtggaca 32940

gttcgtgaag caagctgtgc aaaatcaaga gggagatgga gagagatgac cccccccccc 33000

ctctggcttc agctcatggt tgttagacgt ggcggttgat gattcttcct tctctggtgt 33060

gatctgatat gatcgacaac acaccatcct tccttttctt cattggtttc ttcacatcta 33120

gaatttactg cctgaccggg aaaaaataag ctaggaactt ctatacatgt gtatgcattt 33180

ctaatcatgt agaagaataa gtgtgtcttg actctagata attttaggta gagtcatgct 33240

cgctctccta cattatgtga tgtaactgaa gtgttgttgt gttgtcatgt atggtcttgg 33300

atcactttct gtcaatatat tactagccca caactactat taatttgatg agatgtatat 33360

aattaaaaat a 33371

<210> 7

<211> 33343

<212> DNA

<213> unknown (Artificial Synthesis)

<400> 7

acgaggaggg agcccttgaa ggcaaaccaa tgcgcaccgc cgcttattca ttaaccaaag 60

acttgcatcc aaccaatccc accacttgtg tctacctctt cgagtcaggc tgatgggagg 120

tgcctgtcag caactctccg tggacggaga tgcgaggacg cctgcgaatg accgcgatcc 180

ggccggcctg ctccagcatg atgatacgga aaaccagcag cagcagcagg cctgctggga 240

gcgcttcctc ctcaaggaga ctctcaacgt cttgcttgtg gagagtgatg attcaaccag 300

gcaggtcgtc agtgccctgc ttcgttgctg catgtaccaa ggtttgtttt atcccaattc 360

atccttcagt cgtttttctg acaggctatg atttcgcata ttcatagcat cgatcataac 420

ttggagctgt ttaacatcta taaataccaa ttcgcagcat atagatatgg aacaccgaaa 480

tgataaacat cggtctcagt ttcagtgttt cataaacatt tgcaaagtat catggataat 540

tgatatattg aaacagtaag gcaactgacc aaatcaactt acaatttcaa ctcgcggttg 600

cccgttagta atctaccaca gctggtacat agtagatggc ctcttgcagc cgaggaagga 660

gcgctaggcg tgacagtggt gtacggactg gattgctccc gatcggtgga gcagggagag 720

cggtagcata ggacatcaca agtgatgacg atgccaacgt ggctgtgcaa caagagccac 780

acttggtagt gtttagggtt ccatgatcag ggtgaccaca tcgggtcggc tatgcctatg 840

aaaatggtgc gtgagggagc aacacctatg gaggccacgc tagaggacct gcaggaagga 900

tacatagtgt tgagtcaatg ctgcgacata gattagcctg ttcatgcaag aatgcaaggt 960

ccgttaggag atggtgtagc aaaaggaaaa atggactcat tgaacacgac atgtcaaaag 1020

atgatgatcc tattggattg caagttgagg caccgatatc ctttaggctc cgtttggatg 1080

tcgatattgg agggcaagga attgaattgg gttcaatacc aaatcaacca tgatattgaa 1140

ccaattctat tgtttggatg tcactgaatt ggagtttgga attgtgcggt ctaatccacg 1200

caataccgag gggtgatgct ctgtattgga aagttgagtt tctagttata gtccaattcc 1260

agggaattgg gtctctgatt ccaaacctca attctatgtg caaccaaaca acaaaatttt 1320

gaaaagctga ttccaattct caattttgtg ttccaatatc tacatccaaa cagggtatta 1380

ggccctcatt ggaatgtggg aatttttgct gtttcctgcg tttttcctgt gaaaatgaac 1440

tgattcttgt aaaattcttg tatgattcct ctgaaattcc tgcattccaa agggggcctt 1500

atagtgagga gagtacccaa gaaagacaca taaaatgtac tgagccgcaa gtttatggct 1560

agtagtggca gaaaggttgg ggtaacaagt acaaccaaaa acacgaagat cattcatcgg 1620

tggggagcca gggaaggtga tgcgtggggt ttagtaattt agtgcctgta ggaaggatgt 1680

taagaaggtt ggtgactgta tgcagggttt cagcccagta atgaggagca aataatgttg 1740

ttgatagatc ggatgatgcg tttagccttg ccattttgag tcgaagtgta ggggcatgac 1800

atgcttgagt tgggcaccat gtgtaaggaa gaaggcgcgg gcgctagatt ctatcaagct 1860

cgtggctatt gtcgcacact cgcactagat ggccttaacg gaggcactga actaagtgga 1920

gacataagaa aaaaaataga gtagggaacg tgtccgactt gagtagcagt ggaaaaatcc 1980

aaaaaaaata tgaacaacca tcacaaataa ccaaatagta tttgtaactg gagacaatgg 2040

caacatgaag tccacaagtc acaatgtata aggttgaaat tattcttaac atgagatgtt 2100

ggcacattga aagaaagatg aacacgacga ccgagctaac acgcatgaca taaagtgctc 2160

atatctttat tacaagactg gatggcagaa taatgaacaa gtttggttag tgcctcgtgg 2220

ctgggatgta tgagatgtct ccagagcggg gatgaggtgc ttttgctttt ggtagcttgg 2280

taagaagagt acggattgca taaatgatgc tttagcggca tggctatgct agcgctcaga 2340

cgtctaatgg gtgctaacgt ttgaacactg gtggggccca ccgttgcaac atatacaaca 2400

tcactttgaa ctatcgcgac atccagttga actgttgcaa catttgaaac atgcatttga 2460

acacataaaa caactataac acacttgcac attcaaaaaa catttgttgc aacatctcga 2520

aaatcccatg caacacagaa acaatgaaca caagtacaca acattataaa tcagaaacca 2580

caacattgtt ggtcatatgg gctaggatgt acaacaacaa ggggattaat ttgaggccca 2640

aattgcaact ctagccattg gcccatcacc tttgagccag ttggagggag gagggaatag 2700

gtccccacct ttggcaccgg tcctatgact cctcgtcctc ctatttccct aggtcatatc 2760

gggctcctcg cgccaaggga tcgaagtccc atgagccccc tctgtgggct gaagtaggga 2820

ggtagtatga gcagccgagg atgatggcgt gcagacatcg ccaccacgga ggccatcgta 2880

tgctccgttg gcctcaactt tgacatactt ggtgaagagt gtggataaga atgagtgagt 2940

ggggaggaga gagcgttgtt gggagtgtgc caaacatgtg ttagatattt ctagcgatgt 3000

ctggatactt ttaaagtagc atttccattg ttaataggcc acctatcggc aacactcatt 3060

gcttggcgaa ggaaactcac attctaatat gtctatattg ttttgaacga actttttagc 3120

attttttttg cattgggtat tttttaaact taaaaatact gaactttgaa atcttggctc 3180

cactactgtg ggagaggtgc cactgtagga gacatgggtg cttaacattg gaggatagta 3240

gagcgatcga cgtgagttgc tagtgctcaa tgtctggagg ggacgagaag atactctcgt 3300

ttttttctcg aacacgtagg agagctgcgt atcattgcat taaaagatag aagaacaata 3360

caggggtcaa aaggtcctac acggaaaaga aagaaagaaa aaacacgaca ctaaaacatc 3420

tactccagag aagcaacaca tgcctcaacc taggtcatga ccataaacta agagcaagga 3480

caaaccctta gcaccagcaa gacaccacaa attacattca tcctcagcta aagacaaggc 3540

cctagcgaca ctaggcagaa cgccttcaaa aacagacacg ttgcagtgtc gccatataca 3600

ccaagctcct aaaatcacta gagttgaagc ctttttggat gcctccatta aggcgagtaa 3660

ttgaagtact gcaccaagcc tcaaaattgc tttcctcgat gcccagggac aacgcagcca 3720

gtcccctctt ctggaagatg ttgaaccaaa attgtctagc gaaaacataa gacaccaaca 3780

aatgatgaat tgtttcgtcc ttttggtcac ataaggggca aaagaccgaa tgtgaaaggc 3840

ctcttttagc caacctatca gctgtccaac accggttgtg catcacaagc caaagaaaaa 3900

acaacatttg gccggcaccc aagattgtca aatgcgctca aaaggaccaa aagaagtaga 3960

gccactgaac aacaaatcat aagccgactt agaggagtac tttccacaat tggaaaggct 4020

ccaaaaatgc ttaccatgaa tcgcatgctg cagcacataa tcatagaaaa cttcccaaag 4080

ggtcaaaaag tccaggatga cctcctagaa aacaaccccc ctgaaacaat tagtccaacg 4140

tgaattttcc aaagcctttt tgggacaagg gcgaccacac gaggggtata gtccaccagt 4200

ctcggagcca acaaccatct atcttgccaa aaagagtagt tttgccatct cccatattaa 4260

aggccataac aaataagaat atggactgca ccatagagtg ggactgcatt ggaagagcag 4320

cccaaggttt taaagagtca gttttttttg caaccacaac catctgccat gcaaggccca 4380

atttaaatac tgaagattgg aaataccaag accctcaaga tctttaggtc gagtggcagt 4440

gggccatgcc actaaacagt gacccccgtt ggcatccttg cgacctgtcc aaagaaaact 4500

acgacgaatc ttgtcaaagg ccttaatcgc ccactttgga agctcaagag ccatagcacg 4560

gtaaataatg gtggtagtga ggacagactg aactaggatg gccctgccag cctagtcatc 4620

aatgatttat tctagaggtt gtcgagaaca actatagcag gattgtccag gtggtggtgc 4680

caaatggagg aagagacgat aaggctagct tgagctttat tctaggggtt gtcgagaacg 4740

actatagtaa tatgatcaag gtggtggtgc taaacgaagg atgagatgat aaggttagct 4800

tgagttgcag gggtggtgac tagggggaag gtgttaagat tattgccatt attcaaacaa 4860

agaattacac acctggttta gaagtgctta aaaaaccaag agtgtcaaat ttgttggaac 4920

aactattgtc gcgcgtgaat taatgaacca aaaggttaca cataagtgat ggtatggcca 4980

gaacattatt tcaaatgaaa tttgattatg gcgtgataag aatgaaagta cctcatgaaa 5040

caaggatact atttgccatt gcctattgtg atgaagtaat gggtatgggg aaggcaaaag 5100

agtagtatac catattttta taattgtggg aggccggact agtatctatc tcccaaggtt 5160

taggagtctt gagggacgtg ttgttaaggg ccaaaattag tcactcttga ccccaaacca 5220

acacttgtta ttgttgttac ttggaagcct acgtcatgga gatgtaggca gactcttatg 5280

tcaatgtggg cctagagaca cagacccaac aagctgttgg agagcaccta ggtgtgtgca 5340

aagtagatgt actcacgtgg atggatgtgc tagccaaggg ttggtgtagt gcacatgcac 5400

aacaaaaata tttgttgaca ataacaacac gtaaggaaag attgccccat tgtattgcac 5460

taaggagtat ctcatgggca ggccaagaaa cacctcaaaa agttggttgt tctatacatg 5520

ccatcgagtg ctaggacaca tgtttgtggc catatgtatc ttgtggaaag tgagtctaga 5580

tggttacaca tataattcaa taatataaaa acgaggtttt ctaacctagg catgtttaaa 5640

tgcatcctca catggagtcg agcctaggac ctaaggtgct actaaggttg ttgcacccac 5700

tgggctacaa accctttcac cctataatct attgagaaca ggaaattgat taagggatgt 5760

ttccacaaga gtaaatttta ccgtcagccc ttgaacttgt tctatgttct catctcggtc 5820

ctagtacttg caaattgcac atttagctcc caaaacttat ctcgaattct tagtgttggc 5880

acttgcgttg cacgcttggc tttctaaact tgttctatgt tttcatatcg gttccagtac 5940

ttgtaaattg cgtgctcaac tctctaaact tatattactg taccatccag gtccaacccc 6000

tgcacactat gttgtcctta cccatcactc cacaagtgca tgtctcacca acgcggctac 6060

tctgtgcttc accatgcttg cctgcctacc tgcgttgcat caccgacaca atgtcatact 6120

accatgccac aatgatgcac taccatgttg tgcccttgcc gaggcgcctc actcccatgg 6180

tggtgtaggc gtacgtccac ttagtgagct tgtcgcgaca agagacgagg aaggttggaa 6240

gggcagagag ctggggtgag gagatcatga tcatgagagc atgcgcaatg ggtgagagaa 6300

aggagacaag gagtgtgtca gtattggagt tgagtgcaat taccgcgggg gcaggggtaa 6360

cgctggtggt gctgcaaggt gtaggagcga gtcgagcaag agcaaagagg gcactgaagg 6420

taggatcatg cctagacact catcttcgag aaagctaggc ttgcacgatt tggtggtgca 6480

aatgagcatg gcaatgggtg tggcaccgcg tcaacaacgc agcgtaggta agcatgggat 6540

ggtagattga tcacggtgca acacgacgca ggtgaggtgt gcacatgcat ggcaatgggg 6600

taaggacggt gtcatgcagg gcgaggggtg gactgggatg gtatagtaat acaatttttt 6660

ggagctgaat gtgcaattta ctagtacgag gatgggaacg agaactcata agtttaggga 6720

gccgagtgtg caacttacaa gtactgggat caggttgaga ccccttaagc ctgcacttat 6780

attattttaa aagacccttt cctagcattt gtttcatatg gaggataatc tcccaagttg 6840

tgtattatct attgaaatgg tgcaactttg ttgttttgag ctgggaacta atttcacttt 6900

ctaaaatctt acatatataa tattttatgt tttctttgat tgtttagtgt ttaatattta 6960

gggtggataa tacctgcttc aagctttggt acatagggac aattgcaaat agaaatggag 7020

taggactttt gattgatacg agtctcaaga acagtgtggt ggatgtgagg aggcaagaag 7080

ataggattat ctttttttct cgaacacgca gaagaactgc acatctttat attaagaaga 7140

agaagaagaa agagaggtcc aaaatagacc gctataaagc caccgaaagg tagcaataat 7200

agttacaaac taatcacctt gagcaacacg ccccctacaa caatgacatc ctctaaggcc 7260

ataagccaac caacccaaca acactagcat ggcaccaaac tcccatttag gattggattt 7320

tgcgatagct tttgacctgt tgatagctaa cacctttaag aagagagact cccatttagt 7380

gacctatttt tagataatgg aaacatagtc cgaccttggc ctcaaacgag gttacgacca 7440

attattacaa ctgaaggcaa catattgcct tgacctatca cacgacctca ccaaagaaca 7500

ccaactacta aatcaaagct acaactaagt taaacgcaac gaagaccccc atccaaaatt 7560

gtgaaagaac tgtagtagca ccaactccac atgacgacat gaagacttaa ttaattcttg 7620

gtcctccctt ctaggctgaa gctgtgtcta gaagtgacac caataagttg tcctgaaggt 7680

tacctgcaaa caagatttag gggacaattt attgaaaaca cctcattcct acttaaccaa 7740

atagcccaac aaattgtcga tgcaatggtt aatagtaagt tgcgtgatct ggacaacagg 7800

ccactcgccc aaaccccaaa caaatcatta gcatcccgag gaggtctcaa tcccgtactt 7860

atttgtaact aatgccaaat gaaagacaca tacaaacacc tgaaaaacag gtgtttcact 7920

gtctccggtc tgttacaaaa gcaacatgtc ttactaccgt tccaattcct cctagccagg 7980

ttatcctttg ttaaaattac acctcttttt aggtaccaca taaaaaaatt tattttcaaa 8040

ggaatcttaa gtttccaaag gtccttgtta tccattccca ccccattgtt gagagttgcc 8100

tgatacaaag actgaacagt aaacaaccca ttggtagtct agttccatct gaacgaatcc 8160

ctattagaat tcaaattagt atgtgctacc agcgatacca gttgaaacca ttggaccccc 8220

agatggtgaa ccaaccccct acgaaaggat atgtttaagg gcaccgtggg cagattgact 8280

ttgtcctcac aaggagagaa gataaacgac catccttgga ttgtaaagtg atacctagga 8340

agcgtgttgt ctctcaacat aagtttgtgg tggcggtctt ttgttttcag gtgtgtgtcc 8400

atagggataa acaagacaag attgcgagaa caagctggtg gaaattgaaa ggggaggagg 8460

gaaaaaagtc ttcaaggact aaaagaggac gtttggaaag aagagatgac acaaacaaca 8520

cgtgggagaa gatttttctc gaaagcgcag gagagctgcg cgaaaatata ttaagaaggg 8580

gaaaaaaagg tccaagaagg accccaagat acagataagg ccgccctatg gcggccaaaa 8640

acaagcataa atgaaaccat ccatgacaaa aacactgcta ccaaaacagc actacaacta 8700

tctagctaac aggtagacct gggatggggg cagtaagcaa ggacaacttc tttgcaccag 8760

ccataaccca aagatcaatc tccaaaccaa tgcttctaat agcagcagcc acactaggac 8820

tcttattgtc aaaaacgcag atagcaacct acgttcggaa ggtggtctca gaggtatgtg 8880

gagtaactaa ggggagtgga ggtgaagcta aagatacttg gtggttaaat gaggaagtcc 8940

aaagagctat taaggagaag aaagaatgct acaaacgcct ataccatgat aggaatgtgg 9000

acaacataga gaagtacaag gtagcaaaga agactacaaa gcaagctcta aatgtggtaa 9060

agggtagggc gtatgaggat ctttaccaac gcttgagtac gaaggaagaa gagaaggaca 9120

tatataggtt ggctaaggtt cgtgagagga agataggaga ctttaaccat gtgaagtgca 9180

ttaaggatga aacagagcac cttttggtga aggaggatgg gaccagacat agatgccaaa 9240

agtattttaa caaattgttc aatggggaca caacctttta gttgaatgac ccttttgatg 9300

acaccataga cgctttgtgc ataggatcca agaatctaag gttacagatt agggaggcat 9360

tgaaaaggat gaatacgagt aaggcgatga gccttgatgg tatcccaatt gaggcatgga 9420

gatgcctcag agacatctgt tgtatgacta accaacttgt tcaaccatat cttccggttg 9480

aacaagatgc ctgacgagtg gagagctgaa gaagtatatt ggtaccgatc aacaagaata 9540

agggagatat ttaaagttgt actaattgtt gaagtatata agtggattgc ctaccttcta 9600

acaatttaat cttttgggtt gaactggtta gtgcgtccac tctaacatgg tatcaaagtt 9660

agaggtctcc taacaattcc atgtttgtcc ctttaattgt ccttctgcac ctacacgtgt 9720

tgaactgtat aaatggattg ggttgaactg gtaagtgcgt tcactctaac atggtatcaa 9780

agcctaaggt ctcgagtttg aatcctggcg gatgctttat ttgtgcctcc gcccctttat 9840

tttcatgttt gtgccttttt ctatagatgc acgtgagtgt gggtgttgaa atgtataaat 9900

ggattgccta ccttcttcta acaacttaag tttttgggtt gaactgatta gtgcgtccac 9960

tctaacacta attacagagg aattaagttg atgagccata gtatgaagct atgggagaga 10020

gagagttatc gagtatcatc tgagaggaat aacgtagatc tctttgaacc aatttggttt 10080

aatgcttgga aggtcaacca tggaaaccat tttttaatat gacaaatgat ggagcaatat 10140

atggaacaaa agaaggatct acacatggtt tttattgact tagagaagac ttatgatgaa 10200

ataccaagta atgttatgtg gtgggctttg gacaaacata aagttccaag gaagtacgtt 10260

ggctcattac ggacgtgtac aaaaatgttg tgactagtgt tcgaataagt gatgatggca 10320

tagatgactt tccaattaga atatgattaa atcaagaatt agctttaagc ttttaccttt 10380

tgccttggtg atgaataagg tcacaaagga catacaaggg tatgtttttt tttacgaatg 10440

atgtagttct agttgatgac gggcatgagt aaatagaaaa tagagttgtg gcgggaggct 10500

ctagaatcca aaggttttag acttactaga actaaaatag aatatatgat atgtgatttc 10560

gacatacaca tgatgaaggt gatgttaatt tggaaggtca agtagtccct aggatggaca 10620

cctttaggta tttaggatca atgctacaga gtgataggga tattgatgaa aatgttaagc 10680

agggtggatg aagtggcatc aagcatctgg cgttctatat gacaagaggg taccacaaac 10740

ttgggcacct cctaagtgtc ggttcttcct ttggctggtg gctcataata ggtgctggac 10800

tgcagacaga cttgaaaaaa gaggactaaa tcatcctcct agatgccctc tttgtgacca 10860

agagcctgaa accatcaacc atcttttggt ttcttgtagc ttttccagat tatattggta 10920

catcactctt cggaaattcg ggctccacat tcttgcccct atccaagatg acacaatctt 10980

cctgcagtgg tggaaacgca ttgctgatat tgtgtcaggc atgaccaaga agggggtaaa 11040

ttgcgttgga tttgtggccc atataagaag ggatcgagtt cagaataatg atatacatga 11100

taggctaggg gtagcgccaa ttgaagaaaa gttgatgttg atccaacatc ggttgaaata 11160

gtttggatat gtccaataga gacctctaga gacgtcagtg catagtggga tcctaaggcg 11220

cgataacaat gagaagatag agaaagaccg aagttgacat gaaaagaagc tgtaaaagga 11280

gacttgaaag gatgtgatat attcaagatt tagtcttagg ccctgtttgg cacagcttat 11340

tttcaacttc ttcacaaatt taagcagagg ttctgccaaa caggcagctt ctgcagcagc 11400

ttatcagttc agctgcttct cagaatacac taaaagcagc caacaagcag aagctgcttt 11460

tcaccagctt ctcagataag ctgctttttc aacaagcacc agctgtgcca aacagggcct 11520

taataggagt gcatggaaaa caactattca tgtgcccgaa tcttgattgt gggttctatt 11580

ggatttttaa ctctagccta cccaacttgc ttgggataaa ggccctgttt ggcacagctt 11640

attttcagct tcttcacaaa tttaagcaga agttctgcca aacagctagc ttctgcagca 11700

gcttatcagt tcagctgctt ctcagaatac actaaaagca gccaacaagc agaagctgct 11760

tttcaccagc ttatcagata agctgctttt tcaacaagca gcagctgtgc caaacagggc 11820

caaaagactt tgttgttgtt gtttctttga ctgattgtta ttgttcttta agaaatttag 11880

ttatgctatc caaccttttt cagttatctc tgccgaaaat ggccagcaag catgggctta 11940

tcttgaagat aagagaaaca acatagatct tgttttgact gaggttttta tgcccggtgt 12000

atctggtatt tctctgctga gtagaatcat gagccacaat atttgcaaga acattccagt 12060

gattagtaag taactttcat ctctacagtg tagttcatat catgattaaa atgttgcttt 12120

catcatttat ttagtcccaa tgcttttagc tgctacactg atgttgtttg tgtgcagtga 12180

tgtcttcgag tgatgctatg agtacagtct ttaaatgttt gtcaaaaggt gctgttgact 12240

ttttagtcaa gcctatacgt aagaatgaac ttaagaacct ttggcagcat gtgtggagac 12300

aacggtgtca cagtgtaagt tgttcatggt ctggtttgaa tattgtaagt tctttgacgt 12360

tattcatgtt tctctgggta aaattgtata attactctaa caagagttta cttatttggg 12420

aaacaagaag acacctattg aggtatggtc tggtaaaaca taaggctgtc cctttagacg 12480

tgatgagctc tcccgctccc agcccctctc cctcccccag cccctctccc tctccccctc 12540

acacctccac cagcctctcg ccggaatcca ctccggaccc gtcgaccaac tcttcggccc 12600

ccaccccctc ggagattgcc cccagtcagg ccagtcgccc gatcccccag gaggcaggac 12660

gttctaagtc ccagagatgg tgtgatgacg ttattgatgg tgactcatcg ggaggatcct 12720

cctctgctta tcattcctat aaagaagctc tccttcaacc ttctggtcat ggtggtaacg 12780

cccctgctcc ccctattccc cacttgaaga cgaaagctgg tgaccagtca gaggtgcaag 12840

ggtcttctgc tgaaagtcga cgtcgaaagg aggatggctg gcaggtggtt gagcatagga 12900

ggcgccacct tcctcgtcga ggatccatag tggatctccg agggaagtgc tacaagtgcc 12960

tttctacctc ccacctggta gcttcctgcc gacgtcctac gaggtgctat cgctgcttca 13020

agtttggcca tcaagcggca aggtgcccct tgccggaagc tgggcgaaag acagtgtggc 13080

agcggctggg ctctggtgga gatcgtactc cggtgtggct gcgtttgtct ggagtggaca 13140

attctggccc aacggtgatg gatcgtccag tcattcagcg caaggcccca gtctggcgaa 13200

ggctttcccc tccagtgaac tccccattag tggaaaaaga agcacagggt ggcatgcttc 13260

tgcctgttgg acgtatgaag aagaagagaa gacgttctaa acgcgggaaa ggcagctcgg 13320

gactgcagtc gggcccaagt cagccgcatc ccaatccccc tgttgctgtc cagagcgcct 13380

tgccgcggaa accttcctgc gtattggagt tttccactga tttggcaaga aaggaagctg 13440

ctcttcggag agccttgttt gtttcggtgg ttggcaccag gccgatggtt cgcggggatg 13500

aggtgattga ggaattggcg cgaagtttca gcatcaaccc tgatgatatg tctatccagc 13560

aggccacgcc ggaagatttc ctactattcc tgcctgatga acacacagcg acccaaattc 13620

ttaacgaagg acgacctttc agaggccctg gattttctct caattttaaa agatggacaa 13680

gattctctca tgcctcgacg acgtctatgg cggcactggt tgatattgag attcgaggaa 13740

ttccagctca tgcttgggat atctccacgg caaaaatgtt actacaggat tcgtgctggg 13800

ttttggaatt gttaagggat tcaggaacag catttctgct acgcgcctgg tgttttgacc 13860

caaaatatct gcattcagaa atggaacttc atatcgtcga gccgggcagt gatgagcaga 13920

agaaacgatg tttgacatac aacatttcca tgacagtgtc atcggtgccc tttcagatga 13980

ccaatagcac tgaaactcta tccccttcct ctgacgatgg tcagtttgat gaaggaaatg 14040

acagtgatcc agctgactcc cagcagcgcc gccttccacc tcagccacct agggaatctg 14100

ttcaccttcg gataggcgcc cagtttgtgt ctggaaggag tagggcgctg tgttcggttg 14160

aggaagctga aaggatgggc ttaggcgcct cttcgggact taatgcacaa cattcaatgc 14220

ggccccttga agattgcccg acctggggag atgagatggg aatgcagctg gtctgtggcg 14280

cctcgggtcc ttccaggcag atatgtggcg ccttgggtcc ttccaggcgg atatgcggcc 14340

agccgtccat tgatgcttgc gttgcccaat taaataacgc acagctcgat gctcgggccg 14400

gctccttcat atcggtggag agatcccagg agtcgccttc cccttcatgg aagcagtgta 14460

gacagttggc taatgaggca cgcgaccttg gggtccgagc tgcctccttt aatgatgaag 14520

aaagaaccaa ggtgccacta gctgttgggt cgctgaatca gttgaggtca gcctcgcccc 14580

agtaccattt agctgctggg ccgctggagg gggaccggac tgattcgccc caagctactg 14640

ggcctttggg atttacgggt gaagcacgcc aggatgatct tgtggctgaa ccggtggatt 14700

gggctacgac ggacctcacc catcaaatgt gcatttctga gactgagagg acgaccttga 14760

ctctgggcga tcatgtggct gcggacagcg gaaggaagtc ctcaaccggg aatttgctac 14820

accaatccat tcactgcact actggcattg aacagccagg gatgctacat attaggggcc 14880

ttcaatcatc caaaaagctg atggtatact cacgaaaaag gttcaacagt agggacaagt 14940

ctggaaatat gaatgaagaa gggagtgggc caatcgcttc tgtactgagg attgatactg 15000

atgaaaatac agtgtccacg gtgcgagagg tcagcctagc tgttactact gatggtagtc 15060

agtcaacttc gcaagagacc caaacaggca gtacgcagct caagaacaaa caattcctta 15120

gcaagctctc caaaaagatt tcaggcctcc ttgcaacacc agaagatccc ccttttatga 15180

cgtctaaaaa tgcccaagtt gcagaaatta tcccacgccg cagccgtcga attgctggcg 15240

tgggagttga atttgacaac atggatttat cctcaagaac cacaaagaaa gttatgcagg 15300

ccttgaaagt aattggggat cctggggcag tcacccagca agccaaagaa gactatctga 15360

aagtattctc cactgaactt cctatttcac atattgaagc gctggcgtcc ttttttggat 15420

gggtggtacc agatgatctc aaggtgggca gcattagtgt tggcattagt gcctgttgag 15480

ttgtcctgta gaggtcgctg atgtaagagg tttgtaatgg atccaaaaaa aatcttgagc 15540

tggaatgtaa ggggtctcaa ttctacatct aggcaatgtt ctgttagaac ttgcattgat 15600

gcttgtggcg cagacgttat ctgtcttcag gaaacaaaaa tgcaggaaat taacagagga 15660

aaggtgctgg ctattttggg aacagatttt gatcagtatg taatgctgcc gtccgagggt 15720

gctagggggg gaattctgat ttcctggaaa aagcatataa aggtgacagg gctgaacaga 15780

gtggattctt atagtatctc cttgcaattc cagagaaata atgggcagtc ttggtggctg 15840

acttgtgtct atggacctca aagtaattct gaaaagatcc tttttcttca agagttaagg 15900

catttcagaa gcatctgctc tgagccttgg gtaatagtgg gagactttaa tctcatctac 15960

aaggttgaag acaagaataa tgataatatt aatagatcta tgatgggtcg cttcagaaaa 16020

tttatcaatg atgttggtct gattgatact cccctaattg gtcgcaaata tacctggtcc 16080

aatcatcaat cttcaccaac actggtccga ctggatagag ttctctgcac tactgattgg 16140

gaagcgttgt tccctaacca tctccttcaa agtgctgctt cagaggactc agatcattgc 16200

ccactgattc ttggacttaa cagtatgtgt tctggcaagc gtcgatttca tttcgaatcc 16260

ttttggccaa aattggaagg gttccaagag gtagttctgg aagcctggaa ttcggttaat 16320

gaaagaagtt gccctttcct gacattcgaa ctgaaattga aatcaactgc caaggctctg 16380

cagagatgga gtgctaagaa ggtaggccat gtgtcctccc agttggcact tgctagagaa 16440

attcttcatc agctggagat tgcccaagac tctcggctcc tgtctccttt ggaggtttgg 16500

ctcttgagag ggcttaaaaa acactcctta gcccttgcct ccctcaaacg cactattgcc 16560

cgcctacggt ccagaatcag ttggataaag gagggcgatg ccaactctaa gcttttccat 16620

atgcatgcta ggcacaggca aaggaagaac tttgttgcca agattgtgtc agggatgaac 16680

actttcactg atcacggcga gaaggctaga gcagtagatg agtttttatt tcaaactttt 16740

gggctgcaag gcggacagat ccttgactgt tgatttggat tacctgggtc taccttctca 16800

tgacctgact gatttagatg cgcagattga tgaaaatgag gttctggaat ccataatgca 16860

actaccttct gataaggctc cggggcctga tggttacaca ggcagatttt ataaagtttg 16920

ttggccaatt atcaagacgg atgtgatggc tgttattgct gcaatttggt gcagaaaatt 16980

ccagcagttt ggaagactta acactgcatt tgttactctt atccctaaga aggagggggc 17040

tgaggaagta aaagactttc gacctatcag tctggtacac agcattgcta aacttgtcac 17100

caagttgatg gccaacagat tgtcccaacg actgcatgat cttgtttctc ttaggcagag 17160

cgccttcatc aagggccgtt tcatccaaga caacttcatg ctggttcagc agactgccag 17220

attgcttcac cagcaaggcc aaccgagggt tcttttgaag cttgacatca ctaaagcctt 17280

cgattctgtt tcttggcctt ttttgcttga agtacttcag agattgggtt tcgggcagat 17340

ttggagggat atcttaagtg gccttttagc aacttcctcc acacaggttt tactcaatgg 17400

agttccgggg aacaccatcc cccacaagag agggctgcgg caaggggatc ccctctcccc 17460

tatgcttttt attttagtga tggacatcct tggttgcatt gtggccaaag ctgatgagga 17520

ggggcagctt gagcctctct cttccaaacc actgcaccac agaatttcgc tatatgctga 17580

tgacgcggtt ctttttttcc acccaaggga gagtgaaatt catactgtcc taggtattct 17640

gaatttgttt ggagaagctt caggattaaa aactaatatt cagaaaagca gtgtgtatcc 17700

aatccgttgt gggcaggatg agctggacat cctcaatgat tggcttcctt gtgaactttc 17760

gtctttccca tgcaaatatc ttggtctacc tttgtcctta aaaaagctgt ccaataatca 17820

gctgcagccc attattgaca aagtggctga tcaactttct gggtggaaag cggacctcat 17880

gacacgggca ggtagaagag tgcaggttca gtacgtgatg actggcatgc ttatatatct 17940

agtcatggca gttgacattc ccacaggctc gctcaaagct atcgacaaga tcagaaaagg 18000

atttctctgg cgtgggagga aagaggtcag aggaggacat tgtttagtgg cttggggaag 18060

agtttgtaga cctcttgaaa ttggtggact tgggatttct agtcttaaag aactaggatg 18120

ggccttaaga atgagatggt tgtggctgcg caaaacggat ccggataagc cttggtcctc 18180

attgcccatg cagatatcca acaaaataaa ggcctttttt tgcaccgccc tgctcactga 18240

aattggaaat gggtcctcaa ccttattttg gctggatcgt tgggtccacg gaaagaatat 18300

tgaagatatt gctcctcgac tgcttgcagc ggtaccaaag agaattagga acagcagaac 18360

tgtgcttgag gccttgacag acaggagatg gctaactgat attaaagggg ccttgacagt 18420

aggtgtcttt gccgatctcc tagatctctg ggatgaactt caaagtgtcc agcttcattc 18480

agacagggag gacaaacata ttttcaggtt cgctaatgat ggaatttatt cagcgaaagc 18540

agcttacaat ggcctcttca tcgggtcgac catggctaag tattgggaac taatttggaa 18600

aacctggtcc cctcccaagt gtaaattctt tttatggctt gctgatcttg gaaggtgctg 18660

gacagcagat cgtttgcaga agagaggtct tagccatcct gacaaatgtg tcctgtgtga 18720

ccaagagcaa gaaaccatag atcacatttt ggtggggtgt gtttttgcta ggagtttttg 18780

gtttcagctg ctgggacagg tcaacttatc tgcctttact cctgtgatgg gagaaactaa 18840

aatgatggag tggtggagca gaagcagtga gcagttacag gggattgcta aaaagggatt 18900

caattctttg attacccttg ggctttggac tctctggaac cacagaaatg gatgtgtttt 18960

tgatagagct acacctagca tggagggtgc tatcagaaga gctgaagaag aaattattat 19020

ttggaatttt gcgggggcca aacatcttgc ccttattaca gctcccattc caggtgtcat 19080

gtagggatct aggagtgagt tcagtagtta acctcactag ggaagggtgt tggttggtta 19140

agtttagatt gtgggggtag gttggtctag tttgctttct ttcttctcct ttttcttttt 19200

ctttttcttt atatttcttt ttctgttgtg tttgtaattg gtctaatttt tagacccttc 19260

ttcttaatac aatgatacgc agctctcctg cgtgttcgaa aaaaaaaaaa gtttacttat 19320

ttggaactca gtccagtggt agtggaagtg aaagtggcat tcagacgcag aagtgtacca 19380

agtcaaaagg tgccaatgaa tccggtaaca acagtggtag caacgacgat gaagctggca 19440

tgggacttaa tgcaagggat gacagtgata atggcagtgg cactcaagta tgggttttga 19500

tacttttttt tacttctcta aagcttatta ctggctgtta tcatgttgtt caaggtatga 19560

acgtagtaac acttagaata caagaaacat tttggcgcaa ctgcctataa tcaccaaatg 19620

gaatgtatta gaggtcagtt cacatagcag ctaaatttaa ttgtcattta taaccacaga 19680

attagacagt gtattgctgt cactactaaa tgttaatttc ttaggataac tatttctata 19740

tatatgctag aggtaccaca ttcctggttg aaacaaatga gtaaattact ctggatctta 19800

atttaattct aaaaaattgc acatgaacta gagaatgcct gtgatgtggt tttatttcac 19860

ctaacacact taagtccatc ttgcctctgt tctattttta aacttgacct gcggggggta 19920

agacagcccc cgggcattgt attaagaaca agaccttctc acacaggtcg agaaaacccc 19980

cgaaccccta ccccacccat acacagcggc atcgaagccc atgtgagaac gaccacgacc 20040

ggggctgagc cttgaacctg tgctttagcg tgggacagac gaggggattt ttttaaccac 20100

agcttgaaat tcgctcccac ggagagtcaa acccaggacc tgaggagtgc tactcagacc 20160

acctaaccaa ctcagctaga ggccctttcg ctctgttcta tttttaggct aaaagaaaaa 20220

tgccgactgt attatcttac tgtttcttga acacacatga gagatatgta tcttatatca 20280

ccccttgtat ttttatacaa ttatatgtta gtttagttgc atccagactt ttgcttaaat 20340

agtagcaagg gttcactgta accagttctt tgttcagaat ccatttagaa atatttggtt 20400

gtggaaaagc ttgaggtgaa ctgtttggtt cagaaacaaa ttattgtttc atatccacag 20460

tccatgagtt ttgacgtcaa aagctcatac tgatagaaaa acagagaccc actggatcag 20520

cctaagtccg acacaacctc aatgatgata agggccctaa ttctaatctg aagggttgag 20580

ggggaaggga aaactagcat acttactgtc caagagtaat ggcggcattg ggcatcgccg 20640

tcgtgcccaa ctgcccatcg ctggtgctcc acctccctgt tgtcctcctc cattgtcctg 20700

ccccatgatt agctggagaa agtgggggct cctcggatcc cggccctgtg ggggtggctg 20760

ggctctatgg acttcatcga ttgtggctct tccggtcgct gagaacgccg ggaattgtgg 20820

tggctagggt tttcgggcgc tagaatgtgt tggcgtctct gctggcctag gcctcctatt 20880

tatgttgtgt tctcgtgacc cggggggggg gggggggcaa ccataggttc ggtttgtgcc 20940

cccgatcagg gcgtgcgaca ccatgcactt ttggggagtt tggtgcttat tcaacttggt 21000

tctgttgctg agtcagtgaa catcctaaca aatacacgta agaaccaagt ttgaaatttt 21060

cacgtttgcc actgatgttc tcaatgccat ttgtccacat gagaacattt tgtaacttag 21120

gagtgatatt tactgtatct tttaaacata aaaaatgttt ttaaacttca gatatgttaa 21180

gttaatttta cttattaaac ttgtcgtttt caataatctt ttgctccttt tgtaaatttc 21240

ttttgaacac cgcttgcatt ttaataaaat gtagtttctc caatgtgttg tgaaaatgca 21300

tatgttgtgt tgaatttgtg gccatacaag aagggatcaa gtttgaaacg atgatatacg 21360

tgatagacta agggtagcac caattgaaga aaagttgttc aacaccgatt gagatggttt 21420

ggacatgttc aatggagatc tctagaggca tcagtttttt ttcttgaacc atgcaggaga 21480

attgtgtcat tttatttaag gaggaaaaat aattacatcg ggtaggcaaa aatgccccac 21540

ccgaacgcca cagacaccca acatgggact actcaaagag gagtcacgac ccggaccaat 21600

agccctaccc agctaagacc tgcgaccttc tattttgctc taggaggttg gcagctccgg 21660

ccctacacca aagcaaacct tcgtcatcta ttaactggag aacccgccga acatctggct 21720

gactggcctc aaacacaaaa gagttcctga atttccaaat ctcccaagcc actaaaatga 21780

acaaaaagtg ttgaaccctt tccgcacttt cttatgcacc atcttgatgg agcgtgccac 21840

taggaaaaga aatgagtatc agctgtagaa ggcgtaagag gggcaaggtt tagggagtga 21900

aggatcatgg tccagatttg tcgtgagaac acacagccca cgaagatgtg ttggatggtc 21960

tcgtccattg ttttcaagtc gaacgacttc ctggtcattc tggctgaccg acttgggcta 22020

ttaatcgtga ttaatcacga ttgatcggac gacttggacg attaatcgtg atcggtccaa 22080

actgacttgg atgattaatc acgattaatt ggacgacttg aaaacagtgg tcttgtttgc 22140

ctgatcacag aaggtatagg ccatggggta tgggagacct cacttggcaa ggcgatccac 22200

cgtccaacaa cggtgatttt ttgcaagcca aaggaagaac atgtacttca agggagccca 22260

gcctttccag atattcttcc aaccattaaa actgacagag tccaaaaaga acatggagta 22320

ggctgacttg ctgctataaa ttccggattg agtgaacttc cagtggtgtt gatcagacac 22380

gccctgctgg agaggcatgt catcaaccaa gtcccaaatc actggatatt caatcagcac 22440

ttgcacagat agagcacctc tgatgtcagc aacccaggtt cttttttttc tcgaacacgc 22500

aggagatttg cgtatcattc cattaataga tagaagaagg tacaacacac cacacacgac 22560

acacacctga cacgacacac acacacatga cacaacaaga cacaagacgc aacacagcaa 22620

ccacacacaa ccactctgaa caagccacta gactatcccc ctggtcaagc ataaacataa 22680

gagttgaaag caggttcaac cctttcgccc cggcactaca ccacatcctg agctcatcgc 22740

cagcgagggt gagagcactc gccaggctcg gcgatctccc attaaacaca cagtcgttgc 22800

gatgacgcca aaggctccaa gctccaagaa taactagaga attgaagccg gattggagat 22860

cacggggaat cttctccaca gccctgctcc accagtcatc aaggctgcaa ttagcattat 22920

ctggtgaaag gttaggcaac ccagacctgc ttaacaagga ataccagaac tgtcgtgcaa 22980

agacgcaaga gatgagaaga tgttgtattg tttcctcttc ttggtcgcaa agggggcaca 23040

cactggggtg gggtagcccc cttctagcaa gacggtctgc ggtccagcat ctgttatgca 23100

tgacaagcca aaggaagaaa ctgcactttg aaggtgccca agattcccag atgcgctcat 23160

atgcgcccaa agaagtggaa ccaagaaata acatatcata agccgacttt gaagagtatt 23220

ggcctgtcgg ggagaatttc cagatatgct tgtctggcac tccaacatta atggagtaat 23280

ctgcaatgag ttcattaaga gttaaaaaat cagagaggac atcccaagtg atggtgcctc 23340

tcaggtcccg aatccaagcc atctccacga caccttcagc caccgttctc ctattggtaa 23400

tcctggtcgg gacgagggcg aaaatatgag gagttagatc ctcaatcctc aaccccatca 23460

accatctgtc cctccaaaat aaggtgttac gaccatcacc aatttctgta atcaaagctg 23520

tagagaggat agatttcaga gtgctggaac tctggagagg taaatcagcc caagacttcg 23580

agtgatctgt tctttccaac cacagccatc taacccgaag ggcccaattc agcttttgta 23640

aatctgagat gccaagccca ccaagctcct ttgggcgtgt cactttgccc caagcgacta 23700

agcagtggcc accgagcaca tccttacgac ctttccaaag aaagccacgt ctaatcttgt 23760

caatggcttt gatagcccac ttaggtaaat ccaatgccat ggcgtgatat ataactgttg 23820

ccgtgagcac atgctgcact tggatggctc tgccagctcg agtcatcagg tcggccttcc 23880

aattaggtaa gaggtccgca agcctatcga ttagtggttg cagctgctcc ttgcttagct 23940

tcctgaggga aagaggcaac ccaggttcta ttgtggagga gggcttgagc cacagtcctg 24000

ttcttgactg ccctctttgg gactgcagag agtagagatg gcgccaaatc agctacgcac 24060

tttccatcga accacctatc tgtccagaat tttgttgaca ttccatctcc aacagtagtg 24120

cgaacaacca tatcaaacaa gacccttgcc tttgacagga caaaaacagg aagcccgccc 24180

aaagtctatt gagaaggttg aagttgatgt gagagactaa aataagattt gaaaagatgg 24240

aatataccca aagatttagc cttgaatagg agcgaataga aaacaactat ccacatgctt 24300

gaatcttgtg gtttatgtta ggttttaact ctagcctgcc tcaacttact tgagactaaa 24360

atgctttgtt gttgttgttg ttgtttctcc aatgtactac taagttatta ctgatatgtc 24420

taaaaggcct ctaacttagt tgattaggtg gtctaagtag cactcctcag gcccggagtt 24480

tgactctccg tgggagcaaa tttcatgacg tggttaaaaa aatcacctcg tcttcccacg 24540

ccaaagcata gttctaaggc ctgcccagtc gcagtcgttt tcaatgggct accgtgccgc 24600

tgtgcatggt ggggtaggga ttcgggggtt ttgtagacct acgtgataat gtcttattaa 24660

tatattgccg tggggcggtc ttatctccgc aggtagagtt ttttctactg ataaacgaca 24720

ttagggaatg ggtactgtat ttacaaaatg aactaagtgc aacatgagat atttggtttg 24780

catattatat cctaccatca cacaactaga cataacctga tatggaaacc aaataaaaga 24840

ttttttcttg cagataataa ctaggtggac taacctttaa taattgtata ccattaccac 24900

ataagcagga aaaaattaca cagtgaagac atagtttggt tatgtgtgaa agacgataac 24960

attatgatat gtggattact tatgaggtcc tcaaaagaac ttatgaggca aaagaagtgc 25020

atattttcaa atttgtagtc ttgaaaacgt gaagctgtat ggtttcctgg caaccttgca 25080

agcacatgta cgtattagga taaactatga tgttttcatg ttgacaggat ttaaacgatc 25140

ttgttggcgc ctttttgggc caaacactaa gggtccctgc ctccctggat cccgctcgcg 25200

gggaacgaga ccttggagaa cctctctgcg agatggtaga taaaatataa gacaaataac 25260

actacctgca atacgctcat gaactttacc tctatgatgg caccttctta ggaccaacta 25320

gcaacgaacc ctagggcctt catgtgaagt gcaagacctc gaattcggct ctgcgaggtg 25380

ccggacgatc tgcggtaggt tgtcggacca tcaacgatag ggcagcggac cgtccgtgac 25440

gacatagaga gctcttgggt gaacctggat ctcgcccctg ggagggatct cgtcaggcct 25500

agggtcttgt tagggtcggc aggccaccca cgacgtctct agatgacgta gagtcgaata 25560

caggtgaaga ttgatatgtg gaaactacga ctaaagctgc actagatcta ctcctaagac 25620

atgaatgata aataaggtaa tgtaaataga ttgattcgat tggaatgtgt tcggtgggtt 25680

ttcaatcggt tgtacccctt tatgtttata gggggaggtc tggacccgtt cctaggcaag 25740

atccaatgga ttcccacggt taggttagga taaccatgca tgggataagt actctcgctc 25800

gagttagtct gatcgtggac tgtccggccg tgcccatgtg ccaaatctgg tgctcaacac 25860

ataccccctg ccttttgatg gagcttggtg aaccaaaagc acctggtact accagaatct 25920

ttcgagaaaa catagattct gcaacttgcc ttgttcccga aatatgaact ctagcccgat 25980

atatgtcatt ggctcttgtg atcagataaa actatttgat agaactctaa tgcacagaga 26040

ccgtttaggt ttgcatcctc ttcaaccata tctacttcat caatatgtca ataggcaaaa 26100

acatgtggtg cgccccagcc taaataagca aacagattgg gcaagtaata cagattcatt 26160

gtcgtatcac ctcacacttg agtatgacaa cacattggcg atgaatagac cggaaagtac 26220

cacgacatcc ctggaggtgg atgagcaggt gaccttggct gcaccacttt tcgggccggt 26280

ttagtcagcc tttgcctttg cacagatcgc ctctttgact gttttattgg ccggtggtgc 26340

aaaacggcct tctccttcat atatttggca agaagttgat caaaagtagg gtcagcactg 26400

ctgagccaac caagtgtctt gggggtgttt tgcttcccag tgttgaagtg tataggtgga 26460

cgaactacct tctcctatca gcttaagctt gggttgaact gattagtgcg tccactctaa 26520

catggtatca tagccagagg tctcgagttc gaatcctggc agaggcttta tttgtgcctc 26580

cacccattta tttccatgtt tgtgtctttc tctttgtctg cgtttgcacc tttctctctg 26640

gctgcacgtg agtgagggtg ttgaaatgta taagtggata aactaccttc tcccatcagc 26700

ttaagctttt aggttgaact ggttagtgca tccactctaa cacccagtac atatttcagg 26760

atgttgtagt ctgacggtct gaggtcgccg ccgcttctag ttgtccgcgg attgtccgac 26820

ctttatggtt ggactattcg catctgttct ggactatccg gccttagtgt ccagaccttc 26880

cggcataccc aggataggtg accgagatcg ggtatccgat cgtgcttccc cccggtgctt 26940

tcagacttat tagcctttct tttgtccgga gcctttcgac taaccactcc acgtgataga 27000

tttggcgtgc gagggtcacc aataataata ttttcgcctt tgcctttatc ggccgcacac 27060

ggccgaacca agactttctt gtctgtcggg ctctaatgtg ttgacaggga catgttgcct 27120

gtcaattttt atttcttgaa atctcaatcg accttcattt atagctgatt gtatttaccg 27180

acggaagaca ttacaatcat tggtatcatg aaaaaaggag ccaacaatgc acacgccttt 27240

tcaatttcta tatcggagga attgtgtgag acaatttaat gttaccattc ttaagcaact 27300

catcaaaaaa attatcacac ttagcaacat taaatgtaaa cttaacttct ccccaccgat 27360

tcttttgaat cggctgtagg gaagagcaag cagaaagttt agccttagca agacaaataa 27420

gcttagcggg atgtacctct tttggctcgt catctgagct actactgcca cgacgctcat 27480

catgacaaga ctgttctttg ctttggcttt cacaagtcaa agcccgctgg tgcaatttag 27540

ttagcgaaaa aactagatcc tgtctaattt ttctttcatg tatggacata acacattgaa 27600

agccaaccct gctagttgct tgtccacgac ctggattcga gaacactggt ttctagtatt 27660

ccagaatctc tagatataat ctttaataga ttcatcgtcc tctttctgga tcgatgccag 27720

atcaactaaa tctaactcgt aatcactaga gaagaagtgt tcatgaaact tatgctataa 27780

atcatcccaa gaattaatcg agttaggagg taacacatca taccaagcga acacagtgcc 27840

agtaagggat agtgagaata ggcgaacacg aaaagcttct gtgtcgtcca attcccccaa 27900

ttgtgctaga aattggccta cgtgttcgtg agtgccttta ttgccttcgc ttgagaattt 27960

ggagaaatct gacatactag tccccggagg atatggaacg gtgtcccatt gagggtcgta 28020

tggcctctga tgggactatc ctaggccgga tacactgaca cctaatttat cccgaaattg 28080

ttcgactatt tcttctcgaa cttcatccat agcgatcgat ggtaacccat cggtcctcgg 28140

gacatggggc tcatgtggtc ggttatcctg atgcgggcct tctgcactag gacgatcaaa 28200

ggtgacgttc ggtataggcc cctgaggcat acctgtttca taccccattg tcgtttggta 28260

tgaggtgaac ctttctggct ccctagggat cgaaaaatat tcaacctcat gcatgggtga 28320

tggcatgcta tgactacgat gtgctgtagg tggggtgtag tgtcgctgag gtgagtagga 28380

aaattgttcc tgacgtgcaa cactctgtcc tgtgtagatg tactcggacc gtccgacctg 28440

ggtggccaga cgatccgcga agggactaga cgacccagta ctgtacccgg actgtccagt 28500

tgtgctggca tctcatgcga gccatgtggc gggctcggtg tggctctgaa ctgcccgacg 28560

taaggtgctg aaaggtctgc tatgaaatcg gactgtccga ggttatatct ggactgaccg 28620

acagtatttg gcaccgcatg tgtgccacat ggcgggctcg cgttgcactg gacagtccga 28680

catatggagc cggacagttc gtgcgatggc cagcggagtc agaaagttca ccgcctaata 28740

gatctgactt aatagatccg atctgatgga tctgatctaa taaatctaat ctcagtcatc 28800

cgcagcggag tcaccaaaaa gtgtgttggc gcctttttgg gccaaacact aatgatccct 28860

agaccccgct cgaggggaac gagaccttgg ggaacctctc tacgagatgg tagataaaat 28920

ataagacaaa taacactacc tataatatgg ttttgaactt cacctctatg ctagcacctt 28980

cttagggtca actagcaatg aaccctaggt ccttcacgtg atgtgcagga ccccaagttt 29040

ggctctgcga ggtgccgatt ggtccgcgat aggttgctgg accgttcgcg gtaggcacca 29100

gaccgtccgc gatagggcgg tagaccatcc acgatggtgc aaagagctct tggatctcgc 29160

cccccgggag ggaccttgtc agggaaagat tctaaggtcg tcatggggcc gacaggccac 29220

ccaagacacc tctagacgat gtggagtcga atagaggtga agattgatat gtggaaacta 29280

cgactaaagc tacgctagat ctactcttaa gaaatgaatg ataaataagg taatgtaaat 29340

aaattggttc gattggattg tgttcggggg ttatcaatcg gtcgtaccct ttatatttat 29400

agggggggtc ttcacccatt cgtaggcgag atccaacaga ttcccacgaa taggttaaga 29460

taaccatgct tgggataagt actttttttc tctccgaaaa atgcaggaga gttgcgtatc 29520

attatattaa gaagaagaaa aggtgaggta aaaagagaac ctcatacaac tccacgcaaa 29580

acccacacac actataatag atgcagattt acaaaaatag acagctagac ccgtgacctt 29640

gaagacccca aaccagaggt caccgattgg tgtgctggag cgctaggatg ttagataatc 29700

cacgggctcg agccctacac caaagatgag actcctcctt gacccaattg attaccaagt 29760

tagtacaagg ctggacaccg ttgaaaacac agcggttgcg gtgattccaa atggcccaag 29820

ctgcaagaat gatcagggaa ttcagaccct tgtgcagccc cttgtatgct ggtccaacag 29880

tagaagagga aactccttct caccaatcct taaaagaaga attatcatgg ccaggagcaa 29940

ggtgttgcag cccaacagat ctcataatct gaaaccaaac ttcccgagag aaaacacagg 30000

agactaacaa atggttcaag gtctcctcct gatctcatag caaacagttt tgggggtgag 30060

gtaacccttt ttttgccaga cgatcagctg tccagcagcg accgtgagcc acaagccgca 30120

tgaagaactt gcattttcca ggtgcccaac atttccagat cctcttccaa gggataagta 30180

ctctcgcccg agttaatctg atcgcggact gtccgagtta tctcgcggac cgtttggggc 30240

acatggccgc accgtccgac cgtgcttagg tgccaaatat ggtgctcaac ggatctagta 30300

gggttgggtg gtgttcagag aatgtaagta catttttatg attatcatga ccattagatc 30360

agcttgcata tgtctgcatg ataacttgtc cagaaagtat tgtggtttat ttgttggtga 30420

tggctaccgt gtcaacgcaa cattggtcca tacctttgat ttgaaatatt tgggtgctgt 30480

taacaaagtg tatacctcca agccaaggta gtaaatgttg gacctgaagt gttaaaacat 30540

ggctcagctg gttgggtggt aggtgtggtc gtgcacccaa tcaccgaagg tacaaggcct 30600

cggcttgaat ttgggtgcct atttctctat tttcattttt aattaaaaaa acgtctagtt 30660

tcttctaggt tggatcttga acatatgaat ttcatgattt aaatcatatt atttttcatt 30720

aacgggttat gaacaaggtg atctattctt ataattcgag atgaactgaa ttttgttttt 30780

ctttctttta ctttagttac atagtcatgt atcaattatt tttgaactaa gccataccta 30840

ttttctgtct ctggtttttc tgtggtaagg tatcttactg acaggtgggt ctaagcccat 30900

gtagcactga aagatctcag tggtgaagtt gctatatata tcggaaatta aagcaatgtt 30960

ccccagtgga gttgctatgt atatatcaaa attaaagcat gggcacatta taacttgttt 31020

ccttgttaat atttcataag gcacagaact catggactaa gcttgctgtg gagatcgaca 31080

gtccacaagc tatgtctctg gaccagttag ccgatccagc caatagcacc tgtgcacaag 31140

taatccactc aaagtcagag atatgtagcc acagatggct accaggtagc agcaacagaa 31200

actgcaagaa gcaaaaatac actaatggta tgccatgctc aactgatgca ctatgcaatt 31260

ttacaaaata aataaacttg ctggccattt tatctgttat gtttgatgct tgatttcaga 31320

tgactttaag ggaaaagact tggagataga tggtcctgga aatctatata tggatcacca 31380

atcttcccca aatgagagac ccatcaaagc agcagatcat gaaaacaatt ccaaggagtc 31440

aatgatggga aatttagggg atgcaactgt tcgagctgct gacctaattg gttcaatggc 31500

caaaaacatg gatgcccaac aggcagcaag agctgcagat acccctaatt tctcttctga 31560

agtgccagaa gggaaaggta agaatggtat gtcatgctca actgatgcac tatgcaattt 31620

tacaaaataa ttaaacttgc tggccatttt atctattaag tttgatggtt gatttcagat 31680

gactttaagg gaaaggactt ggagataggt ggccctagaa atttatatat ggatcaccaa 31740

tcttccccaa atgagagacc catcaaagca gcagatcatg aaaacaactc caaggagtca 31800

atgatagaaa atttagagga gccaactgtt cgagctgctg acctaattgg ttcaatggcc 31860

aaaaacatgg atacccaaca ggcagcaaga gctgcagaag atacccctaa tttctcttcc 31920

aaagtgccag aagggaaagg taagaatgac cagcatgata attatgtttt gccatcactt 31980

gagctgagtt tgaagaggtc gagatcatgt ggagatggtg ccaacgatac agtcaatgac 32040

gatgaacagc ggaatagtgc attaaggcga tcgaatctct cggcttttac aaggtgcaaa 32100

acataatatc agtgtcatta gtgagttatg aaagcaacag tatgttgggt actaacagtt 32160

accactttta ttgcaaggta ccatacatct gcggcttcca atcaaggtgg gacaggattg 32220

gtaggtagct gttcgccgca tgataacagc tcggaggctg tgaaaacgga ttctacttac 32280

aacatgaagt caaattcaga tgctgccgca ataaaacaag gctccaacgg cagtagcaat 32340

aacaatgaca tgggttccac tacaaaggat gttgtgacaa aacccagcac aaataacgag 32400

agggtgatgt tgccctctgc tattaaggct aatggataca cgtcaacatt ccatcctgtg 32460

cagcagtgga tggttccaga taatgcgaca gcagggaaag cgaaggctga tgaagtggcc 32520

aacaatgcag gcagaaatag tcaagcacgg ctgctgcaaa tgctggacgg acagatacag 32580

agatacgggc tatcgataaa agtggacctg gaggtggcag tgggagtggc aatgacacat 32640

atgtcagacg gcttgctgcg agcatgacac cacgacaaga acagctaaag aaatatagag 32700

agaaaaagaa agatcggaac tttgggaaaa aggtagcctg ttttcaattg catattaata 32760

tttattcttt atttgttttc aattgcatat gtttaagaag agagagcatt ggatagaagg 32820

actggtttct ttctgatggc tgtctgccat ttccaggtgc ggtaccagag cagaaagagg 32880

ctagccgacc agcggccaag ggttcgtgga cagttcgtga agcaagctgt gcaaaatcaa 32940

gagggagatg gagagagatg accccccccc ccctctggct tcagctcatg gttgttagac 33000

gtggcggttg atgattcttc cttctctggt gtgatctgat atgatcgaca acacaccatc 33060

cttccttttc ttcattggtt tcttcacatc tagaatttac tgcctgaccg ggaaaaaata 33120

agctaggaac ttctatacat gtgtatgcat ttctaatcat gtagaagaat aagtgtgtct 33180

tgactctaga taattttagg tagagtcatg ctcgctctcc tacattatgt gatgtaactg 33240

aagtgttgtt gtgttgtcat gtatggtctt ggatcacttt ctgtcaatat attactagcc 33300

cacaactact attaatttga tgagatgtat ataattaaaa ata 33343

<210> 8

<211> 21

<212> DNA

<213> unknown (Artificial Synthesis)

<400> 8

taacgagagg gtgatgttgc c 21

<210> 9

<211> 21

<212> DNA

<213> unknown (Artificial Synthesis)

<400> 9

agctgttctt gtcgtggtgt c 21

<210> 10

<211> 924

<212> DNA

<213> Escherichia coli

<400> 10

atgaaagcaa cagtatgttg ggtactaaca gttaccactt ttattgcaag gtaccataca 60

tctgcggctt ccaatcaagg tgggacagga ttggtaggta gctgttcgcc gcatgataac 120

agctcggagg ctgtgaaaac ggattctact tacaacatga agtcaaattc agatgctgcc 180

gcaataaaac aaggctccaa cggcagtagc aataacaatg acatgggttc cactacaaag 240

gatgttgtga caaaacccag cacaaataac gagagggtga tgttgccctc tgctattaag 300

gctaatggat acacgtcaac attccatcct gtgcagcagt ggatggttcc agataatgcg 360

acagcaggga aagcgaaggc tgatgaagtg gccaacaatg caggcagaaa tagtcatcct 420

ggtgacgtgc agagcaacct gacgcaacaa catcgtccaa cccttcatta cgtccatttt 480

gagaatagtg gatcaggtgc cctgcaatgt ggttcctcca atgtatttga tcccccactt 540

gaaggccagg ctaccaataa ctatggtgtg aaagccggga gcaactcagg cagcaacaag 600

gggcagaata atgggagtac agctggtgcg agcacggctg ctgcaaatgc tggacggaca 660

gatacagaga tacgggctat cgataaaagt ggacctggag gtggcagtgg gagtggcaat 720

gacacatatg tcagacggct tgctgcgagc atgacaccac gacaagaaca gctaaagaaa 780

tatagagaga aaaagaaaga tcggaacttt gggaaaaagg tgcggtacca gagcagaaag 840

aggctagccg accagcggcc aagggttcgt ggacagttcg tgaagcaagc tgtgcaaaat 900

caagagggag atggagagag atga 924

<210> 11

<211> 307

<212> PRT

<213> Zea mays

<400> 11

Met Lys Ala Thr Val Cys Trp Val Leu Thr Val Thr Thr Phe Ile Ala

1 5 10 15

Arg Tyr His Thr Ser Ala Ala Ser Asn Gln Gly Gly Thr Gly Leu Val

20 25 30

Gly Ser Cys Ser Pro His Asp Asn Ser Ser Glu Ala Val Lys Thr Asp

35 40 45

Ser Thr Tyr Asn Met Lys Ser Asn Ser Asp Ala Ala Ala Ile Lys Gln

50 55 60

Gly Ser Asn Gly Ser Ser Asn Asn Asn Asp Met Gly Ser Thr Thr Lys

65 70 75 80

Asp Val Val Thr Lys Pro Ser Thr Asn Asn Glu Arg Val Met Leu Pro

85 90 95

Ser Ala Ile Lys Ala Asn Gly Tyr Thr Ser Thr Phe His Pro Val Gln

100 105 110

Gln Trp Met Val Pro Asp Asn Ala Thr Ala Gly Lys Ala Lys Ala Asp

115 120 125

Glu Val Ala Asn Asn Ala Gly Arg Asn Ser His Pro Gly Asp Val Gln

130 135 140

Ser Asn Leu Thr Gln Gln His Arg Pro Thr Leu His Tyr Val His Phe

145 150 155 160

Glu Asn Ser Gly Ser Gly Ala Leu Gln Cys Gly Ser Ser Asn Val Phe

165 170 175

Asp Pro Pro Leu Glu Gly Gln Ala Thr Asn Asn Tyr Gly Val Lys Ala

180 185 190

Gly Ser Asn Ser Gly Ser Asn Lys Gly Gln Asn Asn Gly Ser Thr Ala

195 200 205

Gly Ala Ser Thr Ala Ala Ala Asn Ala Gly Arg Thr Asp Thr Glu Ile

210 215 220

Arg Ala Ile Asp Lys Ser Gly Pro Gly Gly Gly Ser Gly Ser Gly Asn

225 230 235 240

Asp Thr Tyr Val Arg Arg Leu Ala Ala Ser Met Thr Pro Arg Gln Glu

245 250 255

Gln Leu Lys Lys Tyr Arg Glu Lys Lys Lys Asp Arg Asn Phe Gly Lys

260 265 270

Lys Val Arg Tyr Gln Ser Arg Lys Arg Leu Ala Asp Gln Arg Pro Arg

275 280 285

Val Arg Gly Gln Phe Val Lys Gln Ala Val Gln Asn Gln Glu Gly Asp

290 295 300

Gly Glu Arg

305

<210> 12

<211> 3228

<212> DNA

<213> unknown (Artificial Synthesis)

<400> 12

gcatgcctgc agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca 60

tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt 120

tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta 180

caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac 240

aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc 300

cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca 360

tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc 420

tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt 480

tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat 540

taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc 600

cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga 660

agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac 720

cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc 780

cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt 840

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca 900

ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca 960

aatccacccg tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc 1020

tctaccttct ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt 1080

tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga 1140

tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa 1200

tcctgggatg gctctagccg ttccgcagac gggatcgatt tcatgatttt ttttgtttcg 1260

ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt 1320

cgggtcatct tttcatgctt ttttttgtct tggttgtgat gatgtggtct ggttgggcgg 1380

tcgttctaga tcggagtaga attaattctg tttcaaacta cctggtggat ttattaattt 1440

tggatctgta tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa 1500

atatcgatct aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat 1560

gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta 1620

gatcggagta gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg 1680

tgtgtgtcat acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat 1740

aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct 1800

attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt 1860

attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta 1920

gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct 1980

gttgtttggt gttacttctg caggtcgact ctagaggatc catgaaagca acagtatgtt 2040

gggtactaac agttaccact tttattgcaa ggtaccatac atctgcggct tccaatcaag 2100

gtgggacagg attggtaggt agctgttcgc cgcatgataa cagctcggag gctgtgaaaa 2160

cggattctac ttacaacatg aagtcaaatt cagatgctgc cgcaataaaa caaggctcca 2220

acggcagtag caataacaat gacatgggtt ccactacaaa ggatgttgtg acaaaaccca 2280

gcacaaataa cgagagggtg atgttgccct ctgctattaa ggctaatgga tacacgtcaa 2340

cattccatcc tgtgcagcag tggatggttc cagataatgc gacagcaggg aaagcgaagg 2400

ctgatgaagt ggccaacaat gcaggcagaa atagtcatcc tggtgacgtg cagagcaacc 2460

tgacgcaaca acatcgtcca acccttcatt acgtccattt tgagaatagt ggatcaggtg 2520

ccctgcaatg tggttcctcc aatgtatttg atcccccact tgaaggccag gctaccaata 2580

actatggtgt gaaagccggg agcaactcag gcagcaacaa ggggcagaat aatgggagta 2640

cagctggtgc gagcacggct gctgcaaatg ctggacggac agatacagag atacgggcta 2700

tcgataaaag tggacctgga ggtggcagtg ggagtggcaa tgacacatat gtcagacggc 2760

ttgctgcgag catgacacca cgacaagaac agctaaagaa atatagagag aaaaagaaag 2820

atcggaactt tgggaaaaag gtgcggtacc agagcagaaa gaggctagcc gaccagcggc 2880

caagggttcg tggacagttc gtgaagcaag ctgtgcaaaa tcaagaggga gatggagaga 2940

gatgaactag taacagcgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt 3000

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat 3060

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt 3120

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca 3180

aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgg 3228

Claims (5)

1. A method for advancing the flowering phase of corn is characterized in that: the expression and/or activity of the gene coding protein shown as SEQ ID NO.1 in the corn is inhibited by using a CRISPR/Cas9 gene method, and a plant with an early flowering phase of the corn is selected;

wherein the genome target region of the CRISPR/Cas9 method in maize is a combination of sequences shown as SEQ ID NO.2 and SEQ ID NO. 3.

2. A kit for advancing the flowering phase of corn, comprising: including any of the following:

(1) the sequence of the sgRNA molecule is the combination of sequences shown in SEQ ID NO.4 and SEQ ID NO. 5;

(2) a DNA molecule encoding the sgRNA of (1);

(3) a vector expressing the sgRNA of (1).

3. A mutant gene for early flowering phase of maize, which is characterized in that: the sequence of the mutant gene is shown as SEQ ID NO.6 or SEQ ID NO. 7.

4. A primer set for detecting a mutant gene according to claim 3, wherein: the primer pair is a sequence shown in SEQ ID NO.8 and SEQ ID NO. 9.

5. Use of the primer set of claim 4 for detecting the mutant gene of claim 3.

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