CN112646691B - Quick culture apparatus of microbial cell - Google Patents
Quick culture apparatus of microbial cell Download PDFInfo
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- CN112646691B CN112646691B CN202011403045.5A CN202011403045A CN112646691B CN 112646691 B CN112646691 B CN 112646691B CN 202011403045 A CN202011403045 A CN 202011403045A CN 112646691 B CN112646691 B CN 112646691B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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Abstract
The invention relates to a microbial cell rapid culture device, which relates to the technical field of cell culture; a partition block is fixed in the incubator, a cavity between the partition block and the incubator on one side of the partition block is a culture cavity, a culture box is arranged in the culture cavity, a movable mechanism is arranged on the upper side of the culture box, heating mechanisms are arranged on the left side and the right side of the culture box, a three-way joint is fixed at the water outlet end of a water outlet pipe, the other two ends of the three-way joint are fixedly communicated with a water diversion pipe, and the other end of the water diversion pipe is arranged on the upper side of the culture box after penetrating through the rear side wall of the incubator; the movable stop block is driven to move up and down, so that the culture cavity in the culture box is adjusted, external air is isolated, intermolecular expansion of the culture solution is accelerated, and the culture time is accelerated.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a microbial cell rapid culture device.
Background
Cell culture is also called cell cloning technology, the formal term in biology is cell culture technology, and cell culture is an essential process for the whole bioengineering technology and one of the biotechnology, cell culture itself is large-scale cloning of cells, the cell culture technology can be used for culturing a large amount of cells into simple single cells or cells with few differentiation, and obtaining a large amount of cells or metabolites thereof through cell culture, and biological products are obtained from the cells, so the cell culture technology is the most core and basic technology in biotechnology; the existing cell culture device has the defects that the existing cell culture device is only used for a cell culture placement function and cannot accelerate the cell culture process.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the invention provides the microbial cell rapid culture device which is simple in structure, reasonable in design and convenient to use, and the movable stop block is driven to move up and down to adjust a culture cavity in the culture box, so that external air is isolated, intermolecular expansion of a culture solution is accelerated, and the culture time is shortened.
In order to achieve the purpose, the invention adopts the technical scheme that: the water pump is fixed on the liquid tank, the water inlet end of the water pump is communicated with the liquid tank through a water pipe, and the water outlet end of the water pump is fixed with a water outlet pipe; the novel cultivation box comprises a cultivation box body, a water outlet pipe, a water distribution pipe, a cultivation cavity, a cultivation box, a movable mechanism and a heating mechanism, wherein the separation block is fixed in the cultivation box body;
the movable mechanism comprises a movable stop block, guide wheels, a steel wire rope, a scroll and a guide motor, wherein a plurality of fixed blocks are symmetrically fixed on the inner top surface of the culture cavity from front to back, the guide motor is fixed on the rear side of the fixed block positioned on the rear side, a worm is fixed after an output shaft of the guide motor passes through the fixed block on the front side of the guide motor, a worm wheel is meshed with one side of the worm, a rotating shaft is arranged and fixed in the worm wheel in a penetrating manner, the upper end of the rotating shaft is screwed with the inner top surface of the culture cavity through a bearing, a first bevel gear is sleeved and fixed on the rotating shaft, a second bevel gear is meshed with the bottom of the front side of the first bevel gear, the scroll is arranged and fixed in the second bevel gear in a penetrating manner, the front end and the rear end of the scroll are respectively screwed and arranged on the corresponding fixed blocks through bearings, the movable stop block is arranged on the upper side of the culture box, the guide wheels are symmetrically arranged on the left side and the right side of the movable stop block, the guide wheels are fixed on the movable stop block through brackets on the front side and the movable stop block, the guide wheels, the steel wire rope is wound and fixed on the guide wheels, and the other end of the steel wire rope is wound and fixed on the scroll;
the heating mechanism comprises air heaters, air outlet pipes, extension pipes and spray heads, the air heaters are fixed on the incubators on the left side and the right side of the culture cavity, the air outlet pipes are fixed at the air outlet ends of the air heaters, the extension pipes are fixed after the air outlet ends of the air outlet pipes penetrate through the incubators, a plurality of spray heads are embedded and fixed at the other side of the extension pipes, the spray heads are arranged on the outer sides of the culture boxes, and the spray heads are distributed at equal intervals;
the incubator is fixed with a control panel, the water pump, the guide motor and the air heater are all connected with the control panel, and the water pump, the guide motor, the air heater and the control panel are all connected with an external power supply.
Furthermore, the extension pipe on the cover establish and be fixed with several support sleeve, support sleeve fixes on the inside wall of incubator, support sleeve and shower nozzle alternate the setting, support the extension pipe through support sleeve.
Furthermore, a plurality of positioning rails are fixed on the inner bottom surface of the culture cavity, a plurality of positioning grooves are formed in the bottom of the culture box, the culture box is arranged on the positioning rails in a front-back sliding mode through the positioning grooves in the bottom of the culture box, positioning lugs are fixed on the rear sides of the positioning rails, and the rear sides of the culture box are in contact with the positioning lugs; the culture box is guided by the positioning rails, so that the culture box is positioned under the movable stop block when being placed in the incubator, and meanwhile, the culture box is limited by the positioning bumps.
Furthermore, a telescopic pipe is fixed at the water outlet end of the water diversion pipe, a support pipe is fixed at the water outlet end of the telescopic pipe, a driving block is sleeved and fixed on the support pipe, a driving motor is fixed on the outer wall of the incubator at the rear side of the culture chamber, an output shaft of the driving motor penetrates through the rear side wall of the incubator and is arranged in the culture chamber, a screw rod is fixed on the output shaft of the driving motor, a sleeve is screwed on the screw rod through threads, the front end of the sleeve is connected and fixed with the driving block, a support block is fixed at the bottom of the telescopic pipe, a telescopic frame is fixed between the support block and the inner wall at the rear side of the culture chamber and comprises a fixed rod and a fixed column, the fixed column is fixed on the inner wall at the rear side of the incubator, the fixed rod is fixed on the support block, the fixed rod is inserted in the fixed column, and the driving motor is respectively connected with the control panel and an external power supply; start through driving motor, drive the screw rod and rotate, the screw rod rotates and makes the sleeve pipe on it carry out the ascending removal of fore-and-aft direction to make the drive block remove, make the stay tube remove to the upside of cultivateing the chamber, so that arrange the culture solution in the liquid tank to the cultivation box in, the stay tube drives flexible pipe when removing and stretches out and draws back the operation, flexible pipe is at flexible in-process, support through the expansion bracket is stretched out and drawn back, makes flexible pipe more firm in the use.
Furthermore, sliding grooves are symmetrically formed in the fixing columns from top to bottom, sliding blocks are symmetrically fixed at the rear ends of the fixing rods from top to bottom, and the sliding blocks are slidably arranged in the corresponding sliding grooves; the fixed rod is guided by the sliding fit of the sliding block and the sliding groove.
The working principle of the invention is as follows: the liquid in the liquid box is pumped out through the water pump and flows into a culture box in the culture box through the water distribution pipe, in the process, the screw rod is driven to rotate by starting the driving motor, the sleeve is pushed through the threads to move in the front-back direction until the support pipe moves to the upper side of the culture box, and when the support pipe moves, the telescopic pipe is driven to stretch and retract, and meanwhile, the fixing rod and the fixing column are matched in a sliding mode to guide; the guide motors on the left side and the right side are started simultaneously to drive the worms to rotate, the worms drive the worm gears on one side of each worm gear to rotate when rotating, so that the first bevel gear rotates, the first bevel gear rotates to mesh with the second bevel gear to rotate, so that the reel rotates, the reel rotates to wind or unwind the steel wire rope, so that the movable stop block is driven to move in height, and when moving, the air in the culture box is controlled, so that the external air cannot enter the culture box; meanwhile, the hot air blower is started, so that hot air blown out by the hot air blower enters the extension pipe and is sprayed out of the spray head to heat the culture box, the temperature in the culture box is increased, molecular expansion in the culture box is accelerated, and the culture process is accelerated.
After adopting the structure, the invention has the beneficial effects that:
1. the height of the movable stop block is adjusted to isolate the outside air, so that the expansion and decomposition of the culture solution in the culture box are facilitated;
2. the movable stop block is adjusted according to the liquid level height of the culture solution in the culture box, so that the movable stop block can meet the use requirements of the culture solution on different liquid levels;
3. the hot air blowers are arranged on the left side and the right side of the culture box, and the culture box is heated by the hot air blowers, so that the temperature of the culture box is increased, and the expansion speed of the culture solution in the culture box is accelerated;
4. the culture box is guided to be limited by the sliding of the positioning rail and the positioning groove, and is limited by the positioning lug, so that the culture box is arranged under the movable stop block.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic diagram of the present invention.
Fig. 2 is a right side view of fig. 1.
Fig. 3 isbase:Sub>A sectional view taken along linebase:Sub>A-base:Sub>A in fig. 1.
Fig. 4 is a sectional view taken along line B-B in fig. 2.
Fig. 5 is an enlarged view of the portion C in fig. 3.
Fig. 6 is an enlarged view of a portion D in fig. 3.
Fig. 7 is an enlarged view of a portion E in fig. 3.
Fig. 8 is an enlarged view of a portion F in fig. 4.
Description of reference numerals:
the device comprises an incubator 1, a liquid tank 2, a water pump 3, a water outlet pipe 4, a door opening 5, a spacer block 6, a water diversion pipe 7, a culture cavity 8, a culture box 9, a positioning groove 9-1, a movable mechanism 10, a movable stop block 10-1, a guide wheel 10-2, a steel wire rope 10-3, a scroll 10-4, a guide motor 10-5, a fixed block 10-6, a worm 10-7, a worm gear 10-8, a first bevel gear 10-9, a second bevel gear 10-10, a heating mechanism 11, a hot air blower 11-1, an air outlet pipe 11-2, an extension pipe 11-3, a spray head 11-4, a three-way joint 12, a control panel 13, a support sleeve 14, a positioning rail 15, a positioning lug 16, an extension pipe 17, a support pipe 18, a driving block 19, a driving motor 20, a screw 21, a sleeve 22, a support block 23, a telescopic frame 24, a fixed rod 25, a fixed column 26, a chute 26-1 and a slide block 27.
Detailed Description
The invention will be further described with reference to the accompanying drawings.
Referring to fig. 1 to 8, the technical solution adopted by the present embodiment is: the water-saving incubator comprises an incubator 1, a liquid tank 2, a water pump 3 and a water outlet pipe 4, wherein the front side of the incubator 1 is hinged with a door 5 through a hinge, the other side of the door 5 is connected with the incubator 1 through a hasp, the rear side of the incubator 1 is provided with the liquid tank 2, the liquid tank 2 is fixed with the water pump 3 with the model number of 15HG10-8E through screws, the water inlet end of the water pump 3 is communicated with the liquid tank 2 through a water pipe, and the water outlet end of the water pump 3 is fixed with the water outlet pipe 4; the culture box also comprises a partition block 6, a water diversion pipe 7, a culture cavity 8, a culture box 9, a movable mechanism 10 and a heating mechanism 11, wherein the partition block 6 is fixedly welded in the culture box 1, a cavity between the partition block 6 and the culture box 1 on one side is the culture cavity 8, the culture box 9 is arranged in the culture cavity 8, two positioning rails 15 are fixedly welded on the inner bottom surface of the culture cavity 8, two positioning grooves 9-1 are formed in the bottom of the culture box 9, the culture box 9 is arranged on the positioning rails 15 in a front-back sliding manner through the positioning grooves 9-1 in the bottom of the culture box 9, a positioning lug 16 is fixedly welded on the rear side of the positioning rails 15, the rear side of the culture box 9 is arranged in contact with the positioning lug 16, the movable mechanism 10 is arranged on the upper side of the culture box 9, the heating mechanisms 11 are arranged on the left side and the right side of the culture box 9, a three-way joint 12 is fixed at the water outlet end of the water outlet pipe 4, the water diversion pipe 7 is fixedly communicated with the other two ends of the three-way joint 12, the other end of the water diversion pipe 7 is arranged on the upper side of the culture box 9 after penetrating through the rear side wall of the culture box 1, a telescopic pipe 17 is fixed at the water outlet end of the water diversion pipe 7, a support pipe 18 is fixed at the water outlet end of the telescopic pipe 17, a driving block 19 is sleeved on the support pipe 18 and fixed by using an adhesive, a driving motor 20 with the model of 60KTYZ is fixed on the outer wall of the culture box 1 on the rear side of the culture cavity 8 by using screws, an output shaft of the driving motor 20 is arranged in the culture cavity 8 after penetrating through the rear side wall of the culture box 1, a screw 21 is fixed on the output shaft of the driving motor 20 in a welding way, a sleeve 22 is arranged on the screw 21 in a screwing way by threads, the front end of the sleeve 22 is fixed with the driving block 19 by using the adhesive, a support block 23 is fixed with the bottom of the telescopic pipe 17 by using the adhesive, and a telescopic frame 24 is fixed between the support block 23 and the inner wall on the rear side of the culture cavity 8 by using the adhesive, the telescopic frame 24 comprises a fixing rod 25 and a fixing column 26, wherein the fixing column 26 is fixedly adhered to the inner wall of the rear side of the incubator 1, the fixing rod 25 is fixedly adhered to the support block 23, the fixing rod 25 is inserted into the fixing column 26, sliding grooves 26-1 are symmetrically formed in the fixing column 26 from top to bottom, sliding blocks 27 are symmetrically adhered and fixed to the rear end of the fixing rod 25 from top to bottom by using an adhesive, and the sliding blocks 27 are slidably arranged in the corresponding sliding grooves 26-1;
the movable mechanism 10 comprises a movable stop block 10-1, a guide wheel 10-2, a steel wire rope 10-3, a scroll 10-4 and a guide motor 10-5, wherein two fixed blocks 10-6 are symmetrically welded and fixed on the inner top surface of the culture cavity 8 in a front-back manner, the rear side of the fixed block 10-6 positioned on the rear side is fixed with the guide motor 10-5 with the model of 60KTYZ by screws, an output shaft of the guide motor 10-5 penetrates through the fixed block 10-6 on the front side and is welded and fixed with a worm 10-7, one side of the worm 10-7 is meshed with a worm wheel 10-8, a rotating shaft is penetrated and welded and fixed in the worm wheel 10-8, the upper end of the rotating shaft is connected with the inner top surface of the culture cavity 8 in a rotating manner through a bearing, a first bevel gear 10-9 is sleeved and fixedly welded on the rotating shaft, a second bevel gear 10-10 is meshed at the bottom of the front side of the first bevel gear 10-9, a reel 10-4 is penetrated and fixedly welded in the second bevel gear 10-10, the front end and the rear end of the reel 10-4 are respectively screwed on a corresponding fixed block 10-6 through bearings, a movable stop 10-1 is arranged on the upper side of the culture box 9, guide wheels 10-2 are symmetrically arranged on the movable stop 10-1 in the left-right direction, the guide wheels 10-2 are fixedly welded on the movable stop 10-1 through brackets on the front side and the rear side of the guide wheels, a steel wire rope 10-3 is wound and fixedly welded on the guide wheels 10-2, and the other end of the steel wire rope 10-3 is wound and fixedly welded on the reel 10-4;
the heating mechanism 11 comprises an air heater 11-1, an air outlet pipe 11-2, an extension pipe 11-3 and a spray head 11-4, wherein the air heaters 11-1 with the model of CY125 are fixed on the incubator 1 on the left side and the right side of the culture cavity 8 by screws, the air outlet pipe 11-2 is fixed at the air outlet end of the air heater 11-1, the extension pipe 11-3 is fixed after the air outlet end of the air outlet pipe 11-2 passes through the incubator 1, five spray heads 11-4 are embedded and fixed on the other side of the extension pipe 11-3, the spray heads 11-4 are arranged on the outer side of the culture box 9, the five spray heads 11-4 are distributed at equal intervals, four support sleeves 14 are sleeved on the extension pipe 11-3 and fixed by using an adhesive, the support sleeves 14 are fixed on the inner side wall of the incubator 1 by using the adhesive, and the support sleeves 14 and the spray heads 11-4 are arranged alternately;
the incubator 1 is fixed with a control panel 13 by screws, the water pump 3, the guide motor 10-5, the air heater 11-1 and the driving motor 20 are all connected with the control panel 13 by leads, and the water pump 3, the guide motor 10-5, the air heater 11-1, the driving motor 20 and the control panel 13 are all connected with an external power supply by power cords.
The working principle of the specific implementation mode is as follows: the liquid in the liquid tank 2 is pumped out by the water pump 3 and flows into the culture box 9 in the culture box 1 through the water distribution pipe 7, in the process, the screw rod 21 is driven to rotate by starting the driving motor 20, the sleeve 22 is pushed by the screw thread to move in the front-back direction until the support pipe 18 moves to the upper side of the culture box 9, and when the support pipe 18 moves, the telescopic pipe 17 is driven to stretch and contract, and meanwhile, the guide is carried out through the sliding fit of the fixed rod 25 and the fixed column 26; the guide motors 10-5 on the left side and the right side are started simultaneously to drive the worm screws 10-7 to rotate, the worm screws 10-7 drive the worm gears 10-8 on one side of each worm screw 10-7 to rotate when rotating, so that the first bevel gear 10-9 rotates, the second bevel gear 10-10 is driven to rotate by meshing when the first bevel gear 10-9 rotates, so that the reel 10-4 rotates, the reel 10-4 rotates to wind or unreel the steel wire rope 10-3, so that the movable check block 10-1 is driven to move in height, and when moving, the movable check block 10-1 controls air in the culture box 9, so that external air cannot enter the culture box 9; meanwhile, the hot air blower 11-1 is started, so that hot air blown out by the hot air blower 11-1 enters the extension pipe 11-3 and is sprayed out of the spray head 11-4 to heat the culture box 9, so that the temperature in the culture box 9 is increased, the molecular expansion in the culture box 9 is accelerated, and the culture process is accelerated.
After adopting above-mentioned structure, this embodiment beneficial effect does:
1. the height of the movable stop block 10-1 is adjusted to isolate the outside air, so that the expansion and decomposition of the culture solution in the culture box 9 are facilitated;
2. the movable stop block 10-1 is adjusted according to the liquid level height of the culture solution in the culture box 9, so that the culture solution on different liquid levels can be used;
3. the hot air blowers 11-1 are arranged on the left side and the right side of the culture box 9, and the hot air blowers 11-1 are used for heating the culture box 9, so that the temperature of the culture box 9 is increased, and the expansion speed of the culture solution in the culture box is increased;
4. the culture box 9 is guided to be limited by the sliding of the positioning rail 15 and the positioning groove 9-1, and the culture box 9 is limited by the positioning bump 16 and is arranged right below the movable stop block 10-1.
The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent substitutions made by those skilled in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (2)
1. A microbial cell rapid culture device comprises an incubator (1), a liquid tank (2), a water pump (3) and a water outlet pipe (4), wherein a door (5) is hinged to the front side of the incubator (1) through a hinge, the other side of the door (5) is connected with the incubator (1) through a hasp, the liquid tank (2) is arranged on the rear side of the incubator (1), the water pump (3) is fixed on the liquid tank (2), the water inlet end of the water pump (3) is communicated with the liquid tank (2) through a water pipe, and the water outlet end of the water pump (3) is fixed with the water outlet pipe (4); the method is characterized in that: the novel culture box is characterized by further comprising a partition block (6), a water distribution pipe (7), a culture chamber (8), a culture box (9), a movable mechanism (10) and a heating mechanism (11), wherein the partition block (6) is fixed in the culture box (1), a cavity between the partition block (6) and the culture box (1) on one side of the partition block is the culture chamber (8), the culture box (9) is arranged in the culture chamber (8), a plurality of positioning rails (15) are fixed on the inner bottom surface of the culture chamber (8), a plurality of positioning grooves (9-1) are formed in the bottom of the culture box (9), the culture box (9) is arranged on the positioning rails (15) in a front-back sliding mode through the positioning grooves (9-1) in the bottom of the culture box, a positioning bump (16) is fixed on the rear side of the positioning rails (15), and the rear side of the culture box (9) is arranged in contact with the positioning bump (16); a movable mechanism (10) is arranged on the upper side of the culture box (9), heating mechanisms (11) are arranged on the left side and the right side of the culture box (9), a three-way joint (12) is fixed at the water outlet end of the water outlet pipe (4), a water diversion pipe (7) is fixedly communicated with the other two ends of the three-way joint (12), and the other end of the water diversion pipe (7) is arranged on the upper side of the culture box (9) after penetrating through the rear side wall of the culture box (1);
an extension tube (17) is fixed at the water outlet end of the water diversion tube (7), a support tube (18) is fixed at the water outlet end of the extension tube (17), a driving block (19) is sleeved and fixed on the support tube (18), a driving motor (20) is fixed on the outer wall of the incubator (1) at the rear side of the incubation cavity (8), an output shaft of the driving motor (20) penetrates through the rear side wall of the incubator (1) and is arranged in the incubation cavity (8), a screw rod (21) is fixed on the output shaft of the driving motor (20), a sleeve (22) is screwed on the screw rod (21) through threads, the front end of the sleeve (22) is connected and fixed with the driving block (19), a support block (23) is fixed at the bottom of the extension tube (17), an extension frame (24) is fixed between the support block (23) and the inner wall at the rear side of the incubation cavity (8), the extension frame (24) comprises a fixed rod (25) and a fixed column (26), the fixed column (26) is fixed on the inner wall at the rear side of the incubator (1), sliding chutes (27-26) are symmetrically arranged in the fixed column (26), and sliding chutes (27-26) are symmetrically arranged in the fixed in the slide grooves (27), the fixed rod (25) is guided through the sliding fit of the sliding block (27) and the sliding groove (26-1), and the driving motor (20) is respectively connected with the control panel (13) and an external power supply; the driving motor (20) is started to drive the screw rod (21) to rotate, the screw rod (21) rotates to enable the sleeve (22) on the screw rod to move in the front-back direction, so that the driving block (19) moves, the supporting pipe (18) moves to the upper side of the culture cavity (8), the culture solution in the liquid tank (2) is discharged into the culture box (9), the supporting pipe (18) drives the telescopic pipe (17) to perform telescopic operation when moving, and the telescopic pipe (17) is telescopically supported by the telescopic frame (24) in the telescopic process, so that the telescopic pipe (17) is more stable in the using process;
the movable mechanism (10) comprises a movable stop block (10-1), a guide wheel (10-2), a steel wire rope (10-3), a scroll (10-4) and a guide motor (10-5), wherein a plurality of fixed blocks (10-6) are symmetrically fixed on the front and back of the inner top surface of the culture cavity (8), the rear side of the fixed block (10-6) positioned on the rear side is fixed with the guide motor (10-5), an output shaft of the guide motor (10-5) penetrates through the fixed block (10-6) on the front side and then is fixed with a worm (10-7), one side of the worm (10-7) is meshed with a worm wheel (10-8), a rotating shaft is arranged and fixed in the worm wheel (10-8) in a penetrating manner, the upper end of the rotating shaft is connected with the inner top surface of the culture cavity (8) in a rotating manner through a bearing, a first bevel gear (10-9) is sleeved and fixed on the rotating shaft, a second bevel gear (10-10) is meshed with the bottom of the front side of the first bevel gear (10-9), two bevel gears (10-10) are arranged in a penetrating manner, two ends of the scroll (10-4) are fixed in a penetrating manner, the movable stop block (10-1) and a left and right movable stop block (10-10) is arranged on the movable stop block (10), and a left and right stop block (10) respectively arranged on the movable stop block (10-6), the guide wheel (10-2) is fixed on the movable stop block (10-1) through brackets at the front side and the rear side of the guide wheel, a steel wire rope (10-3) is wound on the guide wheel (10-2) and fixed, and the other end of the steel wire rope (10-3) is wound on the reel (10-4) and fixed;
the heating mechanism (11) comprises an air heater (11-1), an air outlet pipe (11-2), an extension pipe (11-3) and spray heads (11-4), wherein the air heaters (11-1) are fixed on the incubator (1) on the left side and the right side of the culture cavity (8), the air outlet pipe (11-2) is fixed at the air outlet end of the air heater (11-1), the extension pipe (11-3) is fixed after the air outlet end of the air outlet pipe (11-2) penetrates through the incubator (1), the spray heads (11-4) are embedded and fixed at the other side of the extension pipe (11-3), the spray heads (11-4) are arranged on the outer side of the culture box (9), and the spray heads (11-4) are distributed at equal intervals; a plurality of supporting sleeves (14) are sleeved and fixed on the extension pipe (11-3), the supporting sleeves (14) are fixed on the inner side wall of the incubator (1), the supporting sleeves (14) and the spray heads (11-4) are arranged alternately, and the extension pipe (11-3) is supported through the supporting sleeves (14);
a control panel (13) is fixed on the incubator (1), the water pump (3), the guide motor (10-5) and the air heater (11-1) are all connected with the control panel (13), and the water pump (3), the guide motor (10-5), the air heater (11-1) and the control panel (13) are all connected with an external power supply.
2. The device for rapidly culturing the microbial cells according to claim 1, wherein: the working principle is as follows: liquid in the liquid tank (2) is pumped out by a water pump (3) and flows into a culture box (9) in the culture box (1) through a water distribution pipe (7); the guide motors (10-5) on the left side and the right side are started simultaneously to drive the worms (10-7) to rotate, the worms (10-7) drive the worm wheels (10-8) on one side of each worm wheel to rotate when rotating, so that the first bevel gear (10-9) rotates, the first bevel gear (10-9) rotates by meshing to drive the second bevel gear (10-10) to rotate, so that the scroll (10-4) rotates, the scroll (10-4) rotates to wind or unwind the steel wire rope (10-3), so that the movable check block (10-1) is driven to move in height, and when the movable check block (10-1) moves, the air in the culture box (9) is controlled, so that the external air cannot enter the culture box (9); meanwhile, the hot air blower (11-1) is started, so that hot air blown out by the hot air blower (11-1) enters the extension pipe (11-3) and is sprayed out of the spray head (11-4) to heat the culture box (9), so that the temperature in the culture box is increased, the molecular expansion in the culture box (9) is accelerated, and the culture process is accelerated.
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| CN116333883B (en) * | 2023-05-24 | 2023-08-18 | 苏州贝茵科技股份有限公司 | Carbon dioxide incubator for bioengineering |
| CN118006461B (en) * | 2023-12-28 | 2024-08-30 | 武汉奇凯信息科技发展有限公司 | Cell separation culture method based on visual accurate identification |
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