CN112629935A - Portable large-flow bioaerosol enrichment and liquid phase acquisition device - Google Patents
Portable large-flow bioaerosol enrichment and liquid phase acquisition device Download PDFInfo
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- CN112629935A CN112629935A CN202011635137.6A CN202011635137A CN112629935A CN 112629935 A CN112629935 A CN 112629935A CN 202011635137 A CN202011635137 A CN 202011635137A CN 112629935 A CN112629935 A CN 112629935A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention provides a portable large-flow bioaerosol enrichment and liquid phase acquisition device which comprises an upper cover, a silica gel sealing ring, a sampling cylinder, a base, a control module, a power module, a turbofan, an acquisition pipe and a touch panel, wherein the upper cover is connected with the base; the sampling device comprises a sampling cylinder, a turbine fan, a silica gel sealing ring, a base and a sampling device upper cover, wherein the sampling device upper cover is positioned on the base, the turbine fan is fixed in the upper cover, the silica gel sealing ring is fixed at the lower end of the turbine fan, the bottom end of the sampling cylinder is connected with a collecting pipe and is placed in the base, and when the upper cover is fastened to the base, the sampling cylinder is tightly matched; the structure of the sampling cylinder is as follows: the sampling cylinder consists of a sampling straight cylinder and a bottom conical cylinder, and the collecting pipe is connected to the bottom of the conical cylinder; the top of the sampling straight cylinder is provided with an air inlet channel, the air inlet channel is tangent to the inner wall of the sampling straight cylinder, the top of the sampling straight cylinder is provided with an exhaust channel, and the exhaust channel is parallel to the inner wall of the sampling straight cylinder. The device has high collection efficiency, the flow of collection, sample liquid concentration to avoid the cross contamination of secondary sampling.
Description
Technical Field
The invention belongs to the technical field of bioaerosol samplers, and particularly relates to a portable large-flow bioaerosol enrichment and liquid-phase acquisition device.
Background
In recent years, large-scale infectious diseases such as COVID-19, SARS, H1N1, Ebola, African swine fever, etc. have been developed at a pace. The research on the generation, diffusion and transmission of the bioaerosol is strengthened in all countries around the world. Bioaerosol sampling techniques and devices are important devices for studying bioaerosols. The method can efficiently and quickly collect the microbial particles in the air, and is an important link for researching bioaerosol. With the development of rapid detection technologies, such as PCR, nucleic acid loop-mediated isothermal amplification technology, and enzyme-linked immunosorbent assay, rapid sampling equipment is urgently needed to sample bioaerosol and identify bioaerosol by matching with rapid detection equipment.
The conventional liquid phase sampler SASS2300 large-flow portable full-automatic biological aerosol sampler SASS2300 is a high-efficiency, portable, multi-stage and wet-wall cyclone sampler, and can quickly collect biological particles from aerosol and simultaneously convert the biological particles into a liquid sample for subsequent detection and analysis. 1. The sampling flow rate exceeds 300L/min; 2. automatically sampling and forming a liquid sample; 3. automatic water replenishing, and automatic continuous operation can be carried out for 24 hours; 4. the volume is small, the weight is light, and the device is portable; 5. the built-in charging battery is continuously operated for 24 hours after being fully charged; 6. can be operated manually, automatically or remotely in a wireless way; 7. the user can set the unattended operation automatically by himself.
The problems of the current liquid phase bioaerosol sampler are as follows: firstly, the sample solution is large in volume, usually 20-30ml, so that the concentration of the sample solution is not high, false negative is easy to appear when the sample solution is directly used for detection, and the sample solution is usually required to be concentrated and then detected; secondly, the polluted parts of the sampler are inconvenient to disassemble and clean, so that the collected sample liquid is cross-polluted, and the detection result of the sample liquid is easy to have false positive.
Disclosure of Invention
In view of this, the invention provides a portable large-flow bioaerosol enrichment and liquid-phase collection device, which improves the collection efficiency, the collected flow and the sample liquid concentration of the collection device, and avoids cross contamination of secondary sampling.
The technical scheme for realizing the invention is as follows:
a portable large-flow bioaerosol enrichment and liquid phase acquisition device comprises an upper cover, a silica gel sealing ring, a sampling cylinder, a base, a control module, a power module, a turbo fan, an acquisition pipe and a touch panel; the sampling device comprises a sampling cylinder, a turbine fan, a silica gel sealing ring, a base and a sampling device upper cover, wherein the sampling device upper cover is positioned on the base, the turbine fan is fixed in the upper cover, the silica gel sealing ring is fixed at the lower end of the turbine fan, the bottom end of the sampling cylinder is connected with a collecting pipe and is placed in the base, and when the upper cover is fastened to the base, the sampling cylinder is tightly matched; the touch panel is fixed on the upper cover or the base, and the control module and the power supply module are arranged in the upper cover or the base; the sampling cartridge has the structure that: the sampling cylinder consists of a sampling straight cylinder and a bottom conical cylinder, and the collecting pipe is connected to the bottom of the conical cylinder; the top of the sampling straight cylinder is provided with an air inlet channel, the air inlet channel is tangent to the inner wall of the sampling straight cylinder, the top of the sampling straight cylinder is provided with an exhaust channel, and the exhaust channel is parallel to the inner wall of the sampling straight cylinder.
Furthermore, the turbofan is connected to the upper cover through threads or fixed to the upper cover through a silica gel sealing ring.
Furthermore, the base and the upper cover are connected into a whole which can be opened and closed; the connection between the upper cover and the base can be a clamping connection or a hinge connection; the fastening means being in the form of a clasp or buckle
Furthermore, the power module and the control module are fixed inside the base of the sampling device, the touch panel is fixed on the upper cover and is connected with the control module, the control module is connected with the turbofan, and the power module supplies power to the touch panel, the control module and the turbofan.
Furthermore, the bottom of the cone cylinder is also provided with an extension cylinder, and the extension cylinder extends into the collection tube and is in threaded connection with the collection tube.
Furthermore, the cross section of the silica gel sealing ring is designed into an I-shaped circular double-layer silica gel ring, the silica gel sealing ring is clamped on the upper cover with the circular hole, so that the turbine fan is fixed on the upper cover, and a raised sealing line is designed and processed on the sampling cylinder and is used for being matched and sealed with the silica gel sealing ring.
Advantageous effects
Firstly, the sampling cylinder is structurally designed, so that the sampling cylinder can enrich particulate matters in air and concentrate aerosol to the bottom of the sampling cylinder, the design has the advantages of high enrichment efficiency, the flow of equipment can be changed by changing the size of the sampling cylinder according to requirements without being influenced by the amount of collected liquid, and a large-flow sampler can be designed.
Secondly, the sampling cylinder and the collecting pipe are designed separately and can be disassembled and combined with the base of the sampler, and the sampling cylinder and the collecting pipe can be replaced in the continuous sampling process, so that the cross contamination of secondary sampling is avoided, and the false positive of a sample liquid detection result is avoided.
Drawings
FIG. 1 is a view showing an overall structure of an acquisition apparatus;
FIG. 2 is a schematic diagram of a sampling module;
FIG. 3(a) is a schematic view of a cartridge, and FIG. 3(b) is a cross-sectional view A-A of FIG. 3 (a);
FIG. 4 is a schematic view of a sample outlet;
FIG. 5 is a drawing showing the overall dimensions of the cartridge
FIG. 6 is a diagram of cartridge inlet dimensions;
FIG. 7(a) is a plan view of the seal ring, and FIG. 7(b) is a sectional view A-A of FIG. 7 (a);
FIG. 8 is a sampling schematic;
the device comprises a silica gel sealing ring 1, an upper cover 2, a base 3, a sampling cylinder 4, an air inlet channel 5, an exhaust pipe 6, a sample outlet 7, a vortex cyclone 8 and a collecting pipe 9.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention.
The embodiment provides a portable large-flow bioaerosol enrichment and liquid phase acquisition device, which comprises an upper cover 2, a silica gel sealing ring 1, a sampling cylinder 4, a base 3, a control module, a power module, a turbofan 8, an acquisition pipe 9 and a touch panel. As shown in fig. 1 and 2, the upper cover 2 of the sampling device is located on the base 3, the turbo fan 8 is fixed in the upper cover 2, the silica gel sealing ring 1 is fixed at the lower end of the turbo fan 8, the bottom end of the sampling cylinder 4 is connected with the collecting tube 9 and is placed in the base 3, and when the upper cover 2 is fastened to the base 3, the sampling cylinder 4 and the turbo fan 8 are tightly matched through the silica gel sealing ring 1, as shown in fig. 3(a) - (b). The touch panel is fixed on the upper cover 2 or the base, and the control module and the power supply module can be arranged in the upper cover or the base. Wherein, the structure of sampling cartridge is: the sampling cylinder 4 consists of a sampling straight cylinder and a bottom conical cylinder, the bottom of the conical cylinder is provided with a sample outlet 7, and the collecting pipe 9 is connected to the bottom of the conical cylinder; the top of the sampling straight cylinder is provided with an air inlet channel 5, the air inlet channel is tangent to the inner wall of the sampling straight cylinder, the top of the sampling straight cylinder is provided with an exhaust channel 6, and the exhaust channel 6 is parallel to the inner wall of the sampling straight cylinder.
In this embodiment, the turbo fan 8 may be screwed to the upper cover 2, or may be fixed to the upper cover 2 by a seal ring.
In the embodiment, the base 3 and the upper cover 2 are connected into a whole which can be opened and closed through hinges, and when the sampling cylinder 4 is opened, the sampling cylinder can be taken out from the base 3, so that the disassembly function of the pollution part is realized; when the sampling device is closed, the upper cover 2, the sampling cylinder 4 and the collecting pipe 9 can form a sealed whole, so that the sampling function is realized.
In this embodiment, the connection between the upper cover 2 and the base 3 may be a snap connection or a hinge connection; the two parts can be fastened by a pulling buckle or a buckle. In this embodiment, the upper cover 2 is preferably tightly connected and fixed to the base 3 through the rotating shaft and the pull buckle.
In this embodiment, the power module and the control module are fixed inside the sampling device base, the touch panel is fixed on the upper cover, the touch panel is connected with the control module, the control module is connected with the turbofan 8, and the power module supplies power to the touch panel, the control module and the turbofan.
In this embodiment, as shown in fig. 4, an extension cylinder is further disposed at the bottom of the cone, and the extension cylinder extends into the collection tube 9 and is connected with the collection tube 9 by screw threads.
In this embodiment, the design size of the sampling cylinder is as follows, but the size of the sampling cylinder can be adjusted, so as to adjust the flow.
As shown in fig. 5, cartridge size: intake passage 5 is tangent with the straight section of thick bamboo inner wall of sampling is the volute form, and the air current is followed straight section of thick bamboo inner wall tangential by the air inlet and gets into the sampling cylinder, and the straight section of thick bamboo of sampling is long 125mm, internal diameter 57mm, and the bottom awl section of thick bamboo is 13 degrees with the central line, and the awl section of thick bamboo is long 105mm, and exhaust passage 7 exceeds the air inlet in stretching into the sampling cylinder along 4mm down, and exhaust passage diameter 30mm goes up out appearance mouth diameter 12.5mm on the sampling cylinder 4, and 4 bottom design internal threads of sampling cylinder are connected with 5ml external screw thread.
As shown in fig. 6, the inlet size: the air inlet on the air inlet channel 5 on the sampling cylinder 4 is rectangular, and has the length of 22mm +/-10 mm, the optimal length of 22mm, the width of 14mm +/-10 mm and the optimal width of 14 mm.
As shown in FIG. 5, the sampling cylinder 4 is connected with the collection tube 9 and designed into an extension cylinder with an outer diameter of 14.3mm and a height of 5mm, and the extension cylinder extends into the collection tube 9, so that particles are prevented from being gathered to a connecting gap in the sampling process, and aerosol is fully recovered into the collection tube 9.
As shown in fig. 7(a) - (b), in this embodiment, the cross section of the silica gel sealing ring 1 is designed as an i-shaped circular double-layer silica gel ring, and the silica gel sealing ring 1 is clamped on the upper cover 2 with a circular hole, so that the turbofan 8 is fixed on the upper cover 2, and the air inlet of the turbofan 8 is sealed by the silica gel sealing ring 1; through drawing the knot, link up upper cover 2 and base 3 together for the another side of the turbofan 8 on the upper cover 2 is sealed with sampling cylinder 4, designs the bellied sealed line of processing on sampling cylinder 4, is favorable to strengthening sampling cylinder 4 and silica gel sealing washer 1's leakproofness.
The working principle of the collecting device is as follows:
as shown in fig. 8, a rechargeable lithium battery drives a turbine fan to exhaust air to form negative pressure in a sampling cylinder, the sampling cylinder is designed by using a cyclone centrifugal separation principle, gas entering the sampling cylinder is subjected to solid-gas separation, aerosol in the air is enriched in an acquisition pipe at the bottom of the sampling cylinder, the aerosol-enriched air sample forms a cyclone in the acquisition pipe, and particulate matters in the cyclone tangentially impact the acquisition liquid cyclone in the rotation process and are captured by the acquisition liquid.
The working principle of enriching aerosol of the sampling cylinder is as follows:
wind power generated when the turbine fan operates is utilized, and the silica gel sealing ring is used for connecting the turbine fan and the sampling cylinder, so that great negative pressure is formed in the sampling cylinder, and aerosol in the air can enter the sampling cylinder due to the relation of the negative pressure. When aerosol enters the cyclone sampling cylinder from the tangential air inlet, the air flow changes from linear motion to circular motion, and most of the rotating air flow spirally flows downwards along the inner wall of the sampling cylinder and flows towards a cone, which is generally called as outward rotating air flow. The particle-containing aerosol generates centrifugal force in the rotating process, and particles with large relative density are thrown to the inner wall surface of the sampling cylinder. Once the particles contact the wall of the sampling tube, they lose their radial inertia and fall down the wall by virtue of downward momentum and gravity into the collection tube. When the outward rotating airflow which rotates and descends reaches the cone, the outward rotating airflow is close to the center of the sampling cylinder due to the contraction of the cone. According to the principle of constant rotation moment, the tangential speed is continuously improved, and the centrifugal force applied to the particles is continuously enhanced. When the airflow reaches a certain position at the lower end of the cone, the airflow continuously performs spiral motion from the lower part to the upper part of the small part of the sampling cylinder in the same rotation direction to form internal rotation airflow. A portion of the non-trapped particulates are exhausted through the exhaust passage.
The collecting pipe collects aerosol according to the working principle:
the aerosol is collected into liquid by using a small-volume collecting pipe and adding a small amount of collecting liquid and applying the wet-wall cyclone and tangential impact principle. As shown in fig. 4, the gas enriched with high concentration aerosol rotates at high speed in the collecting tube to form a cyclone, which drives the collecting liquid in the collecting tube to rotate in the same direction to form a cyclone. A part of particles in the cyclone tangentially impact the collected liquid cyclone in the rotating process and are captured by the collected liquid; a portion of the aerosol impinges on the collecting tube wall and adheres to the wall, and a portion of the small particle aerosol escapes with the airflow.
Different from the mode that aerosol is directly collected into liquid by wet wall cyclone, the sampling cylinder is designed to be capable of enriching particulate matters in air and concentrating the aerosol to the bottom of the sampling cylinder. The design has the advantages that the enrichment efficiency is high, the flow of the device can be changed by changing the size of the sampling cylinder according to requirements without being influenced by the amount of the collected liquid, and the large-flow sampler can be designed.
Because the design of gathering the pipe utilizes cyclone striking principle to gather, its collection efficiency is high, and sampling stability, aerosol escape is few, and it is few to gather the liquid measure, and sample liquid volatilizees the concentration at the collection process, and sample liquid concentration is high, can directly be used for the inspection, and the positive rate of testing result is high.
In the embodiment, the sampling cylinder and the collecting pipe are designed in a split mode and can be detached and combined with the base of the sampler, and in the continuous sampling process, the sampling cylinder and the collecting pipe can be replaced, so that cross contamination of secondary sampling is avoided, and false positive of a sample liquid detection result is avoided.
In summary, the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A portable large-flow bioaerosol enrichment and liquid phase acquisition device is characterized by comprising an upper cover, a silica gel sealing ring, a sampling cylinder, a base, a control module, a power module, a turbofan, an acquisition pipe and a touch panel; the sampling device comprises a sampling cylinder, a turbine fan, a silica gel sealing ring, a base and a sampling device upper cover, wherein the sampling device upper cover is positioned on the base, the turbine fan is fixed in the upper cover, the silica gel sealing ring is fixed at the lower end of the turbine fan, the bottom end of the sampling cylinder is connected with a collecting pipe and is placed in the base, and when the upper cover is fastened to the base, the sampling cylinder is tightly matched; the touch panel is fixed on the upper cover or the base, and the control module and the power supply module are arranged in the upper cover or the base; the sampling cartridge has the structure that: the sampling cylinder consists of a sampling straight cylinder and a bottom conical cylinder, and the collecting pipe is connected to the bottom of the conical cylinder; the top of the sampling straight cylinder is provided with an air inlet channel, the air inlet channel is tangent to the inner wall of the sampling straight cylinder, the top of the sampling straight cylinder is provided with an exhaust channel, and the exhaust channel is parallel to the inner wall of the sampling straight cylinder.
2. The portable mass-flow bioaerosol enrichment and liquid phase acquisition device according to claim 1, wherein the turbo fan is connected to the upper cover through a thread or fixed on the upper cover through a silica gel sealing ring.
3. The portable mass flow bioaerosol enrichment and liquid phase collection device of claim 1, wherein the base and the upper cover are connected into an openable whole; the connection between the upper cover and the base can be a clamping connection or a hinge connection; the two parts can be fastened by a pulling buckle or a buckle.
4. The portable large-flow bioaerosol enrichment and liquid phase acquisition device according to claim 1, wherein the power module and the control module are fixed inside a sampling device base, the touch panel is fixed on the upper cover and connected with the control module, the control module is connected with the turbofan, and the power module supplies power to the touch panel, the control module and the turbofan.
5. The portable mass-flow bioaerosol enrichment and liquid phase collection device according to claim 1, wherein the bottom of the conical cylinder is further provided with an extension cylinder, and the extension cylinder extends into the collection pipe and is in threaded connection with the collection pipe.
6. The portable large-flow bioaerosol enrichment and liquid phase acquisition device according to claim 1, wherein the cross section of the silica gel sealing ring is designed to be an I-shaped circular double-layer silica gel ring, and a raised sealing line is designed and processed on the sampling cylinder for being matched and sealed with the silica gel sealing ring.
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| CN202010956215 | 2020-09-11 | ||
| CN2020109562156 | 2020-09-11 |
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| CN202023323785.6U Active CN214471933U (en) | 2020-09-11 | 2020-12-31 | Portable large-flow bioaerosol enrichment and liquid phase acquisition device |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113109101A (en) * | 2021-04-15 | 2021-07-13 | 中国人民解放军海军特色医学中心 | Microbial aerosol sampling unit and device |
| CN114088477A (en) * | 2021-12-07 | 2022-02-25 | 北京慧荣和科技有限公司 | Wet-wall cyclone type air aerosol sampler |
| CN114544272A (en) * | 2022-02-11 | 2022-05-27 | 北京大学 | Cyclone type sand-dust aerosol grading sampling device, method and application |
| CN116818971A (en) * | 2023-06-06 | 2023-09-29 | 苏州智爱环境科技有限公司 | Online organic aerosol thermal desorption gas chromatography mass spectrometry analysis system and method |
| CN114088477B (en) * | 2021-12-07 | 2024-06-07 | 北京慧荣和科技有限公司 | Wet wall cyclone type air aerosol sampler |
-
2020
- 2020-12-31 CN CN202011635137.6A patent/CN112629935A/en active Pending
- 2020-12-31 CN CN202023323785.6U patent/CN214471933U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113109101A (en) * | 2021-04-15 | 2021-07-13 | 中国人民解放军海军特色医学中心 | Microbial aerosol sampling unit and device |
| CN114088477A (en) * | 2021-12-07 | 2022-02-25 | 北京慧荣和科技有限公司 | Wet-wall cyclone type air aerosol sampler |
| CN114088477B (en) * | 2021-12-07 | 2024-06-07 | 北京慧荣和科技有限公司 | Wet wall cyclone type air aerosol sampler |
| CN114544272A (en) * | 2022-02-11 | 2022-05-27 | 北京大学 | Cyclone type sand-dust aerosol grading sampling device, method and application |
| CN114544272B (en) * | 2022-02-11 | 2023-12-01 | 北京大学 | Cyclone type sand aerosol hierarchical sampling device, method and application |
| CN116818971A (en) * | 2023-06-06 | 2023-09-29 | 苏州智爱环境科技有限公司 | Online organic aerosol thermal desorption gas chromatography mass spectrometry analysis system and method |
| CN116818971B (en) * | 2023-06-06 | 2024-04-05 | 苏州智爱环境科技有限公司 | Online organic aerosol thermal desorption gas chromatography mass spectrometry analysis system and method |
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| CN214471933U (en) | 2021-10-22 |
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