CN112625956A - Method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry - Google Patents
Method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000012258 culturing Methods 0.000 title claims abstract description 17
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 13
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/441—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/444—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by ultrafiltration or microfiltration
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
Abstract
The invention discloses a method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry, which comprises the steps of detecting the content of nitrogen, phosphorus and potassium and PH in the membrane concentrated biogas slurry, diluting the concentrated biogas slurry according to the content of nitrogen, phosphorus and potassium, directly using the diluted membrane concentrated biogas slurry as a culture solution for culturing the photosynthetic bacteria, wherein the inoculation amount is 10% of the volume of the culture solution, placing the inoculated membrane concentrated biogas slurry under the illumination intensity of 3000-5000Lux, and culturing the membrane concentrated biogas slurry at the temperature of 30 ℃ for 5-7 days. The method adopts the membrane-concentrated biogas slurry as the culture solution of the photosynthetic bacteria, the nutrient substances of the concentrated biogas slurry are richer without artificially adding nutrient substances, the dilution factor of the concentrated biogas slurry is determined by detecting various indexes such as nitrogen, phosphorus and potassium salinity of the concentrated biogas slurry, the growth requirement of the photosynthetic bacteria is met, and most microorganisms and all solid particles in the biogas slurry are removed by the membrane-concentrated biogas slurry.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry.
Background
Photosynthetic bacteria are prokaryotic organisms that can use light energy as an energy source, and are all gram-negative bacteria, which are classified into oxygen-producing cyanobacteria and non-oxygen-producing purple bacteria. Commonly used are rhodopseudomonas palustris of the genus rhodospirillum and rhodopseudomonas capsulata among the purple bacteria. Rhodopseudomonas palustris is mainly used in low salinity water bodies, and Rhodopseudomonas capsulata is mainly used in high salinity water bodies. Rhodopseudomonas palustris for use in the present invention. The organic matter heterotrophic culture medium can not only perform phototrophic culture without oxygen release under the condition of oxygen deficiency, but also perform heterotrophic culture by utilizing organic matters under the conditions of darkness, no illumination and oxygen. Can be widely used in rivers, lakes, ponds, marshes and other places.
Photosynthetic bacteria can utilize substances such as organic matters, ammonium nitrogen, hydrogen sulfide, nitrite and the like in nature, and can utilize a wide range of nutrients, so that the photosynthetic bacteria can also be widely present in soil of rivers, lakes and seas. The photosynthetic bacteria have wide substance decomposition and utilization, contain rich nutrient substances, have protein content of over 60 percent in cell dry matter, contain various vitamins, wherein the vitamin content of B group is the most abundant, and the contents of folic acid, pantothenic acid and biotin are also higher. Meanwhile, the photosynthetic bacteria also contain physiological active substances such as carotenoid and coenzyme Q, so that the photosynthetic bacteria have high nutritional value. The feed additive not only can purify water quality and reduce substances such as ammonia nitrogen and nitrite in the water body, which are harmful to aquatic animals, but also can promote growth of poultry and fish and prevent diseases.
The excrement of laying hens of a chicken house is divided into two types, one type is dry excrement, and the other type is wet excrement. The dry manure is directly collected by farmers, but wastes time and labor, is not handled completely, but has small volume and relatively small amount; the wet manure is the manure directly washed by tap water for the layer raiser, the manure flows into the manure storage tank along with the water tank, and the methane engineering company can transport the manure by using a manure trolley, so that the advantages of time and labor saving are achieved, the chicken coop is clean, and most of the manure of the layer raiser is wet manure. Because the water amount of each farmer is different, the nutrient content in the wet manure is also different; the wet manure has different nutrient contents in different seasons, has larger difference, is thick and thick in manure in autumn and winter, has hot weather in spring and summer, has large rainfall, has large tap water flushing amount, and can cause the manure to flow into a manure storage pool to be thin. Therefore, the nutrient content of the biogas slurry at different time is different, and the nitrogen, phosphorus, potassium, salinity and PH difference of the biogas slurry in different seasons are large, so that the detection of the nitrogen, phosphorus, potassium, salinity and PH value of the membrane concentrated biogas slurry is very important when the membrane concentrated biogas slurry is used as a photosynthetic bacteria culture solution. The growth of photosynthetic bacteria can be inhibited when the nitrogen, phosphorus and potassium content and the salinity content in the membrane concentrated biogas slurry are too high, and the concentration of the photosynthetic bacteria can not meet the use requirement when the concentration is too low.
The photosynthetic bacteria can perform photoautotrophy under the condition of oxygen deficiency and illumination, and can perform heterotrophic growth and propagation by using organic matters under the condition of oxygen deficiency and no illumination. The seed propagation of the photosynthetic bacteria adopts the condition of dark and aerobic condition for propagation, the propagation is carried out on a shaking table at 30 ℃ and 180rpm, the propagation requirement can be met by culturing the viable count for two days, and the production time of the photosynthetic bacteria can be greatly shortened.
The membrane-concentrated biogas slurry contains the following nutrient components: 5.86-6.88 g/L of ammonia nitrogen, 1.40-1.82 g/L of phosphorus, 3.55-5.57 g/L of potassium, 0.42-1.02 g/L of organic matters, 6.02-8.55 g/L of carbonate and bicarbonate, 22.0-30.4 per mill of salinity and 8.11-8.95 of PH.
After the biogas slurry is subjected to multi-stage precipitation, multi-stage filtration, ultrafiltration, reverse osmosis and other treatments, not only are all solid particles and most microorganisms removed, but also the biogas slurry is changed into clear and transparent light yellow from the original opaque dark brown. The simply filtered biogas slurry is dark gray or dark brown and is light-proof, and has a small amount of solid particles and a large amount of microorganisms, so that the culture of photosynthetic bacteria is obviously seriously influenced.
Disclosure of Invention
In order to solve the problems in the prior art, the technical scheme of the invention provides a method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry. The technical scheme is as follows:
the method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry is characterized by comprising the following steps of: step A, activating and expanding culture of photosynthetic bacteria seeds:
a1, quickly thawing a glycerol tube of the photosynthetic bacteria preserved at ultralow temperature, adding the glycerol tube of the photosynthetic bacteria preserved at ultralow temperature into a sterilized first quantitative photosynthetic bacteria activation liquid culture medium on a super clean bench after thawing, performing shake culture at the temperature of 30 ℃ and the rotation speed of 180rpm for 48h, and transferring a second quantitative activation liquid culture medium on the super clean bench after 48h to continue shaking culture for 48 h;
a2, after carrying out shake flask propagation to a third fixed quantity, continuing propagation for 48 hours by adopting a fermentation tank, and adopting an activation culture medium as a culture medium;
a3, after the expansion culture of the fermentation tank is finished, adopting an expansion culture medium to perform expansion culture, filling the expansion culture medium into a colorless transparent plastic bottle, culturing for 5-7 days at the inoculation amount of 10 percent and the illumination intensity of 3000-;
step B, preparing membrane concentrated biogas slurry culture solution:
b1, performing anaerobic fermentation on the feces of the poultry farm to obtain biogas slurry, and performing separation and concentration treatment on the biogas slurry through membrane concentration equipment to finally obtain transparent, clear and light yellow membrane-concentrated biogas slurry;
b2, at least measuring the indexes of nitrogen, phosphorus, potassium, PH, ammonia nitrogen and salinity, diluting the membrane concentrated solution by 3-5 times, adjusting the PH to 7.5-8.0, and putting the membrane concentrated solution into a barrel for later use as a culture solution for fermentation of photosynthetic bacteria;
step C, culturing photosynthetic bacteria:
and (3) inoculating the photosynthetic bacteria seed solution prepared in advance into the culture solution in the step B2, wherein the inoculation amount is 10% of the volume of the culture solution, placing the photosynthetic bacteria seed solution under the illumination intensity of 3000-.
Further, the first amount in the step A is 100ml, the second and third amounts are 1L, and the certain time in the step C is 5-7 days.
Further, in the step A1, the method for quickly thawing the ultralow-temperature preserved photosynthetic bacterium glycerol tube is to put the photosynthetic bacterium glycerol tube into a constant-temperature water bath kettle at 37 ℃ for quick thawing for 2-3 min.
Further, in the step a1, the formula of the liquid culture medium for activating photosynthetic bacteria is as follows: 0.8-1.2g/L of sodium acetate, 0.3-0.6g/L of sodium propionate, 0.1-0.3g/L of potassium dihydrogen phosphate, 0.4-0.8g/L of ammonium sulfate, 0.8-1.2g/L of yeast extract and 0.2-0.5g/L of beef extract, and the pH value is adjusted to 7.5-8.0 by using 30% of sodium hydroxide.
Further, in the step a3, the formulation of the culture medium for expanding photosynthetic bacteria is as follows: 0.6-1.0g/L of sodium acetate, 0.2-0.5g/L of sodium propionate, 0.2-0.8g/L of potassium dihydrogen phosphate, 0.2-0.8g/L of potassium dihydrogen sulfate, 0.6-1.0g/L of ammonium chloride, 0.1-0.3g/L of sodium thiosulfate, 0.2-0.5g/L of citric acid, 0.002-0.005g/L of ferrous sulfate, 0.1-0.3g/L of magnesium sulfate, 0.2-0.5g/L of sodium carbonate and 7.5-8.0 of PH.
Further, in the step B1, the poultry is a laying hen.
Further, in the step B1, the excrement of the laying hen farm enters an anaerobic fermentation tank through a pipeline for anaerobic fermentation to generate biomass gas, after 3 days of anaerobic fermentation, biogas residues and biogas slurry are separated through a solid-liquid separator, and the biogas slurry sequentially enters three sedimentation tanks a, B and c for sedimentation to remove particles in the biogas slurry; after settling for 12h in each settling tank, allowing the biogas slurry to enter the next settling tank, settling in a settling tank c, and performing multistage filtration by using a coarse filter, a high-efficiency fiber filter and a secondary precision filter to remove small particles which are not settled in the biogas slurry; then, ultrafiltration and reverse osmosis are carried out to obtain light yellow membrane concentrated biogas slurry.
Further, in the step B2, nitrogen, phosphorus and potassium are determined according to a determination method of nutrient elements in the liquid compound microbial fertilizer in the national standard; measuring ammonia nitrogen according to a method for measuring ammonia nitrogen in sewage; the pH was measured with a pH meter, and the salinity was measured with a salinity meter.
Compared with the prior art, the technical scheme of the invention at least has the following beneficial effects:
(1) the photosynthetic bacteria seeds are cultured on the shaking table under the condition of darkness and oxygen, the culture speed of the photosynthetic bacteria seeds is greatly shortened, the photosynthetic bacteria seeds are cultured under the illumination anaerobic condition for at least 5-7 days, and the photosynthetic bacteria seeds are cultured under the dark anaerobic condition for only two days.
(2) The nitrogen source of the propagation culture medium is an inorganic nitrogen source, organic nitrogen sources such as yeast extract, protein powder, beef extract and the like are not adopted, the mixed bacteria rate of the photosynthetic bacteria is greatly reduced, and the added ferrous sulfate and citric acid can promote the rapid growth of the photosynthetic bacteria.
(3) The membrane concentration technology is adopted to treat the biogas slurry, the treated biogas slurry is changed into clear and transparent light yellow from opaque dark brown, and illumination can penetrate through the membrane to concentrate the biogas slurry, which is a prerequisite condition that photosynthetic bacteria can normally grow. The traditional biogas slurry and the culture wastewater for culturing photosynthetic bacteria are not decolorized, the color of the biogas slurry is darker, and the growth and proliferation of the photosynthetic bacteria are seriously influenced by the light transmittance difference.
(4) The biogas slurry subjected to membrane concentration treatment removes all insoluble particles and most of bacteria and other microorganisms, and the traditional biogas slurry treatment only filters the insoluble particles and most of bacteria, so that part of the insoluble particles and most of the bacteria are remained, and the growth and long-term storage of photosynthetic bacteria are influenced.
(5) And detecting data of nitrogen, phosphorus, potassium, salinity, PH and the like of the membrane concentrated biogas slurry, adjusting the PH, and determining the dilution factor to enable the membrane concentrated biogas slurry to meet the growth requirement of photosynthetic bacteria. In the traditional method for preparing the photosynthetic bacteria culture solution by using the biogas slurry, the salinity of the components is uncertain, and nutrient substances such as a nitrogen source and the like are required to be added.
(6) The utilization of the biogas slurry is more scientific and reasonable, and the mode that the traditional biogas slurry is too extensive in use is changed.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a technical roadmap for an embodiment of the invention;
FIG. 2 is a growth curve of photosynthetic bacteria in the example of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Fig. 1 and 2 show the technical scheme and photosynthetic bacteria growth curve of example 1 of the present application, wherein the experimental groups: the membrane concentrated biogas slurry is used as a culture medium; control group: the supernatant obtained after biogas slurry filtration and precipitation is used as a culture medium.
Step A: activation and propagation of photosynthetic bacteria seeds
(1) Preparing a photosynthetic bacteria activation culture medium, taking 0.5g of sodium acetate, 0.2g of sodium propionate, 0.3g of monopotassium phosphate, 0.3g of ammonium sulfate, 0.4g of yeast extract, 0.15g of beef extract, 500ml of distilled water and 30% sodium hydroxide to adjust the pH value to be 7.5-8.0, taking 100ml of the mixture, adding the mixture into a 500ml shake flask, plugging the shake flask with rubber plugs and kraft paper, and sterilizing in an autoclave at 121 ℃ for 20 min. Cooling, placing on a super clean bench, and turning on the ultraviolet lamp for 30 min.
(2) Taking out a 5ml photosynthetic bacteria glycerol tube preserved in a-80 ℃ ultra-low temperature refrigerator, quickly thawing in a 37 ℃ constant temperature water bath for 2-3min, adding 100ml photosynthetic bacteria activation liquid culture medium which is sterilized on a super clean bench after thawing, wrapping with a black film, performing shake culture, culturing at 30 ℃ and 180rpm for 48h, transferring 1L sterilized activation liquid culture medium on the super clean bench after 48h, and continuing to perform shake culture for 48h by the same method after wrapping with the black film.
(3) After the flask is shaken to expand to 1L, a 50L fermentation tank is adopted to continue expanding culture. 50L fermenter activated liquid culture25L of culture medium, taking 25g of sodium acetate, 10g of sodium propionate, 7.5g of monopotassium phosphate, 15g of ammonium sulfate, 20g of yeast extract and 7.5g of beef extract, adding 22L of distilled water, adjusting the pH to about 7.5 by using 30% sodium hydroxide, adding 2ml of rapeseed oil for defoaming, sterilizing by using high-pressure steam at the temperature of 121 ℃ and 0.1-0.12MPa for 20min, sterilizing by using 0.12MPa high-pressure steam of an air filter connected with a sterile air pipeline for 15min, cooling the culture medium after the sterilization is finished, and preparing 0.2% of new Gill sterilizing solution, a round towel wet steel ring wrapped with gauze, two double-cotton-thread gloves and 95% ethanol by using a fire circle inoculation method. Closing the window of the room where the fermentation tank is located, sterilizing the fermentation tank and the periphery by using 0.2% benzalkonium bromide, and completely pouring the wrapped gauze steel ring by using 95% ethanol for later use. The method comprises the following steps of putting two cotton gloves with two cotton threads, fully pouring a pair of cotton gloves with tap water, closing a stirrer of a fermentation tank, closing an aseptic air valve, slightly unscrewing a cover of an inoculation port, sleeving a steel ring fully poured with 95% ethanol on the cover, igniting with an ignition gun, quickly unscrewing the cover in fire, opening a bottle stopper of a shake flask beside flame, baking the bottle opening on the flame for a circle, and inoculating photosynthetic bacteria cultured by a 1L shaking table into the fermentation tank. Then baking the can cover on fire for two circles to cover the inoculation port, then quickly moving the fire circle to the ground to cover the wet towel for fire extinguishing, finally screwing the can cover, opening the sterile air valve, and adjusting the ventilation volume to be 1.0m3And h, turning on the stirring rotating speed to be 150rpm, controlling the temperature of the culture medium in the tank to be 29-31 ℃, wrapping the upper surface and the side surface of the fermentation tank with a visible lens by using a black film, and culturing for 48h in the dark.
(4) After the expansion culture of the fermentation tank is finished, the expansion culture medium is adopted for expansion culture, a colorless transparent plastic bottle is used for filling, the inoculation amount is 10%, the culture temperature is 30 ℃, the illumination intensity is 3000-.
And B: preparation of membrane-concentrated biogas slurry culture solution
(1) Anaerobic fermentation is carried out on excrement of a laying hen farm to obtain biogas slurry, multistage sedimentation is carried out on the biogas slurry through a sedimentation tank a, a sedimentation tank b and a sedimentation tank c, ultrafiltration and reverse osmosis treatment are carried out after multistage filtration of a coarse filter, a high-efficiency fiber filter, a secondary precision filter and the like, and finally transparent and clear light yellow membrane concentrated biogas slurry is obtained.
(2) And (3) detecting the membrane concentrated biogas slurry obtained in the step B (1), wherein the detection data is as follows: 6.56g/L of ammonia nitrogen, 1.58g/L of phosphorus, 5.28g/L of potassium, 0.88g/L of organic matters, 8.25g/L of carbonate and bicarbonate, 25.6 per mill of salinity and 8.57 of PH. Adjusting pH to 7.8 with potassium dihydrogen phosphate, diluting the membrane concentrated biogas slurry by 5 times, placing into a white ton barrel, placing into an ultraviolet lamp, and sterilizing under ultraviolet lamp irradiation for 1 hr.
And C: culture of photosynthetic bacteria
Inoculating the photosynthetic bacteria seed solution which is expanded and cultured in advance into the culture solution in the step B (2), wherein the inoculation amount is 10 percent of the volume of the culture solution, placing the photosynthetic bacteria seed solution under the illumination intensity of 3000-600nmValue, OD600nmThe value reaches more than 1.5, and the cultured photosynthetic bacteria can be obtained.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A method for culturing photosynthetic bacteria by utilizing membrane concentrated biogas slurry is characterized by comprising the following steps:
step A, activating and expanding culture of photosynthetic bacteria seeds:
a1, quickly thawing a glycerol tube of the photosynthetic bacteria preserved at ultralow temperature, adding the glycerol tube of the photosynthetic bacteria preserved at ultralow temperature into a sterilized first quantitative photosynthetic bacteria activation liquid culture medium on a super clean bench after thawing, performing shake culture at the temperature of 30 ℃ and the rotation speed of 180rpm for 48h, and transferring a second quantitative activation liquid culture medium on the super clean bench after 48h to continue shaking culture for 48 h;
a2, after carrying out shake flask propagation to a third fixed quantity, continuing propagation for 48 hours by adopting a fermentation tank, and adopting an activation culture medium as a culture medium;
a3, after the expansion culture of the fermentation tank is finished, adopting an expansion culture medium to perform expansion culture, filling the expansion culture medium into a colorless transparent plastic bottle, culturing for 5-7 days at the inoculation amount of 10 percent and the illumination intensity of 3000-;
step B, preparing membrane concentrated biogas slurry culture solution:
b1, performing anaerobic fermentation on the feces of the poultry farm to obtain biogas slurry, and performing separation and concentration treatment on the biogas slurry through membrane concentration equipment to finally obtain transparent, clear and light yellow membrane-concentrated biogas slurry;
b2, at least measuring the indexes of nitrogen, phosphorus, potassium, PH, ammonia nitrogen and salinity, diluting the membrane concentrated solution by 3-5 times, adjusting the PH to 7.5-8.0, and putting the membrane concentrated solution into a barrel for later use as a culture solution for fermentation of photosynthetic bacteria;
step C, culturing photosynthetic bacteria:
and (3) inoculating the photosynthetic bacteria seed solution prepared in advance into the culture solution in the step B2, wherein the inoculation amount is 10% of the volume of the culture solution, placing the photosynthetic bacteria seed solution under the illumination intensity of 3000-.
2. The method according to claim 1, wherein the first amount in step a is 100ml, the second and third amounts are 1L, and the period in step C is 5 to 7 days.
3. The method as claimed in claim 1, wherein the step a1, the method for fast thawing the cryopreserved photosynthetic bacteria glycerol tubes is to put the tubes in a 37 ℃ constant temperature water bath for fast thawing for 2-3 min.
4. The method as claimed in claim 1, wherein in step A1, the photosynthetic bacteria activating liquid culture medium is prepared by the following formula: 0.8-1.2g/L of sodium acetate, 0.3-0.6g/L of sodium propionate, 0.1-0.3g/L of potassium dihydrogen phosphate, 0.4-0.8g/L of ammonium sulfate, 0.8-1.2g/L of yeast extract and 0.2-0.5g/L of beef extract, and the pH value is adjusted to 7.5-8.0 by using 30% of sodium hydroxide.
5. The method according to claim 1, wherein in step A3, the formulation of the culture medium for expanding photosynthetic bacteria is: 0.6-1.0g/L of sodium acetate, 0.2-0.5g/L of sodium propionate, 0.2-0.8g/L of potassium dihydrogen phosphate, 0.2-0.8g/L of potassium dihydrogen sulfate, 0.6-1.0g/L of ammonium chloride, 0.1-0.3g/L of sodium thiosulfate, 0.2-0.5g/L of citric acid, 0.002-0.005g/L of ferrous sulfate, 0.1-0.3g/L of magnesium sulfate, 0.2-0.5g/L of sodium carbonate and 7.5-8.0 of PH.
6. The method according to claim 1, wherein in step B1, the avian is a laying hen.
7. The method according to claim 6, wherein in step B1, the manure of the laying hen farm enters an anaerobic fermentation tank through a pipeline for anaerobic fermentation to generate biomass fuel gas, after 3 days of anaerobic fermentation, biogas residues and biogas slurry are separated through a solid-liquid separator, and the biogas slurry sequentially enters three sedimentation tanks a, B and c for sedimentation to remove particles in the biogas slurry; after settling for 12h in each settling tank, allowing the biogas slurry to enter the next settling tank, settling in a settling tank c, and performing multistage filtration by using a coarse filter, a high-efficiency fiber filter and a secondary precision filter to remove small particles which are not settled in the biogas slurry; then, ultrafiltration and reverse osmosis are carried out to obtain light yellow membrane concentrated biogas slurry.
8. The method according to claim 1, wherein in step B2, nitrogen, phosphorus and potassium are measured according to the method for measuring nutrient elements in the liquid compound microbial fertilizer in the national standard; measuring ammonia nitrogen according to a method for measuring ammonia nitrogen in sewage; the pH was measured with a pH meter, and the salinity was measured with a salinity meter.
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