CN112625253B - Surface polymer brush modified hydrogel material, preparation method and application - Google Patents
Surface polymer brush modified hydrogel material, preparation method and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G81/00—Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers
- C08G81/02—Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers at least one of the polymers being obtained by reactions involving only carbon-to-carbon unsaturated bonds
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- G02C—SPECTACLES; SUNGLASSES OR GOGGLES INSOFAR AS THEY HAVE THE SAME FEATURES AS SPECTACLES; CONTACT LENSES
- G02C7/00—Optical parts
- G02C7/02—Lenses; Lens systems ; Methods of designing lenses
- G02C7/04—Contact lenses for the eyes
- G02C7/049—Contact lenses having special fitting or structural features achieved by special materials or material structures
Abstract
The invention discloses a preparation method of a hydrogel material with a surface polymer brush modified, which takes methacrylic acid-beta-Hydroxyethyl (HEMA) as a main monomer to prepare a cross-linked hydrogel, and changes the physical and chemical properties of the hydrogel by adding a third component. Subsequently, hydroxyl groups on the surface of the hydrogel are activated to amino groups, and coupled with carboxyl groups at the ends of polyglycidyl methacrylate (PGMA) prepared by reversible addition-fragmentation chain transfer (RAFT) radical polymerization, and polymer chains are grafted to the surface of the hydrogel. And further modifying functional molecules on the surface of the hydrogel through the reaction of the small molecules with amino groups and epoxy groups in PGMA. A layer of functional polymer brush is modified on the surface of the hydrogel by a post-modification method, and the length of the polymer brush can be adjusted by the feeding ratio of RAFT polymerization. The invention changes the surface performance of the hydrogel on the basis of keeping the water content and the mechanical property of the hydrogel, and improves the protein adsorption resistance.
Description
Technical Field
The invention belongs to the technical field of functional polymer materials, and particularly relates to a surface polymer brush modified hydrogel material, preparation and application thereof as a corneal contact lens.
Background
With the development of times and the progress of science and technology, the global incidence of myopia is generally on the rise in recent years and reaches 60% -80%. A contact lens is a medical device worn on the cornea of the eye for correcting vision and treating partial eye diseases, and is popular with consumers because of its beauty and convenience. The materials for preparing the contact lens mainly go through four stages, which are respectively: a rigid gas impermeable material, a rigid gas permeable material, a soft non-hydrophilic material, and a soft hydrophilic material. Hydrogels are three-dimensional cross-linked network polymeric materials containing a large number of water molecules. The cornea contact lens prepared by using hydrogel as a soft hydrophilic material has better water content, comfort, light transmittance, wettability, biocompatibility and the like, and is the cornea contact lens material which is most used at present. Despite the great progress made in the correction of vision and the treatment of eye diseases, there still exist a series of disadvantages in the current state of corneal contact lenses, for example, the most widely used material of soft hydrophilic corneal contact lenses, i.e. poly-beta-hydroxyethyl methacrylate (HEMA), has good mechanical properties, optical properties and biocompatibility. However, the material has low water content of about 38 percent, has certain biological adhesiveness, is easy to form protein and lipid deposition in the wearing process, can cause eye dryness and the like after being worn for a long time, can cause eye diseases to be worsened, and limits the wider application of the corneal contact lens. Therefore, there is a need to develop new hydrophilic corneal contact lenses to meet the public needs.
The amphoteric ion polymer is a high molecular material which is electrically neutral as a whole and simultaneously contains anionic and cationic groups on the same monomer side chain. Because of the characteristics of strong hydration capability, good biocompatibility and the like, the biological water-soluble chitosan has been widely researched and applied in the field of biological medicine. Researches show that the hydrogel material prepared by the zwitterionic polymer has good biological adhesion resistance, but when the addition ratio of the zwitterionic monomer such as betaine and phosphorylcholine used for preparing the hydrogel is too high, the hydrogel has unsatisfactory hydrophilicity and reduced mechanical properties.
Surface modification refers to the treatment of the original surface of a material to change its structure and functional groups. The surface modification can endow the material with new performance without affecting the original performance of the material. The plasma method, the surface coating method and the surface grafting method are the commonly used modification methods for the surface of the biomaterial at present. Among them, the surface grafting method has received much attention from people because of its simple method and good surface stability. Therefore, by adopting the surface grafting technology, the zwitterionic molecules are grafted on the surface of the hydrogel, so that the mechanical property and the water content of the original hydrogel are not influenced, the hydrogel is endowed with the characteristics of bacterial adhesion resistance and protein adsorption resistance, the hydrogel is favorable for being worn as a corneal contact lens for a long time, and the incidence rate of ocular inflammation is effectively reduced.
Disclosure of Invention
The invention aims to provide a surface polymer brush modified hydrogel material which has good hydrophilicity, water retention rate and protein adsorption resistance and can be worn as a corneal contact lens for a long time.
The design idea of the invention is as follows: the crosslinked hydrogel is prepared by taking methacrylic acid-beta-Hydroxyethyl (HEMA) as a main monomer, and the physical and chemical properties of the hydrogel are changed by adding a third component. Subsequently, hydroxyl groups on the surface of the hydrogel are activated to amino groups, and coupled with carboxyl groups at the ends of polyglycidyl methacrylate (PGMA) prepared by reversible addition-fragmentation chain transfer (RAFT) radical polymerization, and polymer chains are grafted to the surface of the hydrogel. And further modifying functional molecules on the surface of the hydrogel through the reaction of the small molecules with amino groups and epoxy groups in PGMA, modifying a layer of functional polymer brush on the surface of the hydrogel through a post-modification method, adjusting the length of the polymer brush through the feeding ratio of RAFT polymerization, changing the surface performance of the hydrogel on the basis of keeping the water content and the mechanical property of the hydrogel, and improving the protein adsorption resistance. The specific technical scheme is as follows:
a preparation method of a surface polymer brush modified hydrogel material comprises the following steps:
step 1: preparation of hydrogel material: uniformly mixing the monomer m, the monomer n, the initiator and the cross-linking agent according to a certain proportion, putting the mixture into a mold, putting the mold into an ultraviolet curing reactor or an oven for reaction for 0.2-8 h, and demolding to obtain the hydrogel material A.
Step 2: activating the surface of the hydrogel material: and (3) reacting the hydrogel A with 3-aminopropyltriethoxysilane, and activating hydroxyl on the surface of the hydrogel into amino to obtain a hydrogel material B.
And step 3: preparation of polyglycidyl methacrylate (PGMA): dissolving monomer glycidyl methacrylate, a chain transfer agent and an initiator in an anhydrous solvent, heating for reaction, and preparing PGMA through RAFT free radical polymerization.
And 4, step 4: and grafting the PGMA to the surface of the hydrogel material B through a condensation reaction between carboxyl at the chain end of the PGMA and amino on the surface of the hydrogel material B to obtain the hydrogel material C.
And 5: grafting functional molecules: dissolving the micromolecules with amino groups in the solution, adjusting the pH value of the solution to 9-12, soaking the hydrogel material C in the solution, reacting for 12-48 h, coupling the micromolecules with amino groups to the surface of the hydrogel material C through the reaction of the amino groups of the micromolecules and epoxy groups in glycidyl ester, obtaining a hydrogel material D, and further reacting the hydrogel material D with lactobionic acid to obtain a hydrogel material E.
In the step 1, the monomer m is beta-hydroxyethyl methacrylate (HEMA), and the monomer N includes but is not limited to one or two of methacrylic acid (MAA), Acrylic Acid (AA), acrylamide, N-vinyl pyrrolidone (NVP), 3- (triethoxy silicon) propyl methacrylate, 3-epoxypropyl tris (trimethylsiloxy) silane, N-hydroxymethyl acrylamide, N-hydroxyethyl acrylamide and 2-hydroxypropyl methacrylate.
The cross-linking agent in the step 1 is one or two of Ethylene Glycol Dimethacrylate (EGDMA), diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, polyethylene glycol dimethacrylate, ethylene glycol diacrylate, diethylene glycol diacrylate, triethylene glycol diacrylate, polyethylene glycol diacrylate, glyceryl trimethacrylate, glyceryl triacrylate, glyceryl dimethacrylate and glyceryl diacrylate.
The initiator in the step 1 is one of azo compound initiator, peroxide initiator or ultraviolet initiator.
The mould in the step 1 consists of a convex film and a concave film; the curvature of the inner side of the bottom of the convex die is the same as that of the corneal contact lens, and the convex die is used as a forming die of the corneal contact lens.
And the RAFT chain transfer agent in the step 3 is one of benzyl dithiobenzoate, isobutyl dithiobenzoate, trithiocarbonate, 4-cyanodithiobenzoylvaleric acid or carboxymethyl thiobenzoate.
The initiator in the step 3 is a free radical initiator and comprises Azobisisobutyronitrile (AIBN), Azobisisoheptonitrile (ABVN), benzoyl peroxide tert-butyl ester and methyl ethyl ketone peroxide.
In the step 4, the condensation reaction adopts one or two of 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) and Dicyclohexylcarbodiimide (DCC) as a catalyst, and the reaction time is 12-48 h.
The small molecule with amino group in the step 5 comprises triethylene tetramine, tetraethylene pentamine, alanine, glycine, serine, lysine, arginine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, methionine, threonine, cysteine, tyrosine, asparagine, glutamine, aspartic acid or glutamic acid.
Advantageous effects
1. The hydrogel material prepared by taking HEMA as a main monomer and adding other components has high water content, hydrophilicity and transmittance, and hydroxyl groups contained on the surface can be used for subsequent surface modification.
2. The invention uses RAFT active free radical polymerization method to prepare poly glycidyl methacrylate, and grafts the poly glycidyl methacrylate on the surface of hydrogel. The RAFT polymerization molecular chain is controllable in length and narrow in molecular weight distribution, the prepared hydrogel is uniform in surface performance, the length of the chain segment which can be regulated and controlled is beneficial to researching the influence of the length of the surface graft polymer brush on the comprehensive performance of the hydrogel, and the optimal length of the graft polymer brush is guided to be selected.
3. According to the invention, small molecules such as amino acid or lactobionic acid are grafted on the surface of the hydrogel, the amino acid and lactobionic acid are used as natural products, the biocompatibility is better, and the surface property of the hydrogel can be effectively adjusted by adjusting the types of amino acid molecules.
4. The hydrogel prepared by the invention has good hydrophilicity, water content and mechanical property when being used as a corneal contact lens, and particularly, the surface of the hydrogel is modified by lactobionic acid and optimized amino acid, so that the protein adsorption resistance can be improved, and the hydrogel is favorable for being used as the corneal contact lens for a long time.
Drawings
FIG. 1 is a flow chart of a method for making a contact lens according to example 1 of the present invention;
FIG. 2 is a nuclear magnetic resonance hydrogen spectrum of polyglycidyl methacrylate (PGMA) prepared in example 1 of the present invention;
FIG. 3 is a gel permeation chromatography outflow curve of PGMA in example 1 of the present invention;
FIG. 4 is a photograph of a contact lens prepared in example 2 of the present invention;
FIG. 5 is a flow chart of a method for making a contact lens according to example 5 of the present invention;
FIG. 6 is a graph of protein adsorption data in example 6 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following examples and the accompanying drawings.
Example 1
The experimental flow chart of the preparation of the surface aspartic acid modified corneal contact lens is shown in figure 1.
Step 1:
HEMA (1 mL), methacrylic acid (MAA) (8.5 mu L), crosslinking agent Ethylene Glycol Dimethacrylate (EGDMA) (15 mu L) and photoinitiator 1299 (9.5 mu L) are placed in a corneal contact lens preparation mold, reacted for 30 min in an ultraviolet curing reactor, and placed in a mixed solution of ethanol and water for demolding to obtain the corneal contact lens.
Step 2:
placing the corneal contact lens prepared in the step 1 and 3-aminopropyltriethoxysilane in an acetone solution, stirring at room temperature for 8 h, washing the corneal contact lens with ethanol and water respectively for 2 times after the reaction is finished, soaking in distilled water for 24 h, and changing water every 8 h.
And step 3:
glycidyl Methacrylate (GMA) is taken as a monomer (0.71 g, 5 mmol), RAFT chain transfer agent (61 mg, 0.167 mmol) and AIBN (2.7 mg, 0.0167 mmol) are added into a dry three-neck flask with a preset stirrer, and oxygen in a reaction tube is removed through three times of liquid nitrogen freezing-vacuumizing-melting nitrogen circulation. Tetrahydrofuran (8 mL) was added. The mixture is put into an oil bath kettle at 70 ℃ for reaction for 6 hours, and after the reaction is finished, the atmosphere is introduced and the reaction is quenched in an ice bath. The reaction solution was dropped into n-hexane to precipitate. After purifying the polymer by a two-time dissolution-precipitation method, it was dried to obtain polyglycidyl methacrylate (PGMA). The nuclear magnetism calculated the monomer conversion rate is about 80%, and the polymerization degree is 24. The nuclear magnetic spectrum of the prepared polymer PGMA is shown in figure 2, and the outflow curve of the Gel Permeation Chromatography (GPC) is shown in figure 3. The results showed that PGMA had a number average molecular weight of 5.66 kg/mol, a weight average molecular weight of 4.75 kg/mol, and PDI =1.18, thereby demonstrating the success in preparing monodisperse PGMA with controlled molecular weight by RAFT polymerization.
And 4, step 4:
PGMA (50 mg), N-hydroxysuccinimide (NHS) (26 mg) was placed in a mixed solution of 15 mL ethanol and 15 mL deionized water and reacted for 4 h on a magnetic stirrer. 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) (50 mg) and the contact lens prepared in step 2 were further added thereto, and the reaction was stirred at room temperature for 24 hours. After the reaction was completed, the reaction mixture was washed with ethanol and water 2 times, respectively, and then soaked in water for 24 hours.
And 5:
adding a certain amount of aspartic acid into an ethanol/water (1: 4, v/v) mixed solution to prepare a 0.1 mol/L amino acid aqueous solution, and adjusting the pH value to 10-12. The cornea contact lens prepared in the step 4 was put into the amino acid aqueous solution, and then placed in an incubator and reacted at 50 ℃ for 24 hours. And after the reaction is finished, taking out the corneal contact lens, and washing the pH value of the solution to 7 by using distilled water to obtain the surface aspartic acid modified corneal contact lens.
Example 2
Preparation of surface serine modified corneal contact lens
Step 1: HEMA (1 mL), N-vinyl pyrrolidone (NVP) (12.0 muL), crosslinking agent Ethylene Glycol Dimethacrylate (EGDMA) (12 muL) and thermal initiator AIBN (20 mg) are placed in a corneal contact lens preparation mold, reacted for 8 hours in a 70-DEG constant temperature oven, and placed in a mixed solution of ethanol and water for demolding to obtain the corneal contact lens.
And 5: adding a certain amount of serine into an ethanol/water (1: 4, v/v) mixed solution to prepare a 0.1 mol/L amino acid aqueous solution, and adjusting the pH value to 10-12. The cornea contact lens prepared in the step 4 was put into the amino acid aqueous solution, and then placed in an incubator and reacted at 50 ℃ for 24 hours. And after the reaction is finished, taking out the corneal contact lens, and washing the pH value of the solution to 7 by using distilled water to obtain the surface serine modified corneal contact lens. The photograph of the contact lens obtained is shown in fig. 4, which shows that the contact lens with good shape is successfully prepared.
Example 3
Preparation of surface lactobionic acid modified corneal contact lens
The experimental flow chart is shown in fig. 5. Step 1 and step 2 are the same as those in example 1.
And step 3: glycidyl Methacrylate (GMA) is taken as a monomer (0.71 g, 5 mmol), RAFT chain transfer agent (36.5 mg, 0.1 mmol) and AIBN (2.7 mg, 0.0167 mmol) are added into a dry three-neck flask with a preset stirrer, and oxygen in a reaction tube is removed through three times of liquid nitrogen freezing, vacuumizing and melting nitrogen circulation. Tetrahydrofuran (8 mL) was added. The mixture is put into an oil bath kettle at 70 ℃ for reaction for 8 hours, and after the reaction is finished, the atmosphere is introduced and the reaction is quenched in an ice bath. The reaction solution was dropped into n-hexane to precipitate. After purifying the polymer by a two-time dissolution-precipitation method, it was dried to obtain polyglycidyl methacrylate (PGMA). Monomer conversion was about 78%, degree of polymerization 39.
And 4, step 4: PGMA (50 mg), N-hydroxysuccinimide (NHS) (20 mg) was placed in a mixed solution of 15 mL of alcohol and 15 mL of deionized water and reacted for 4 h on a magnetic stirrer. 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) (40 mg) and the contact lens prepared in step 2 were further added thereto, and the reaction was stirred at room temperature for 24 hours. After the reaction was completed, the reaction mixture was washed with ethanol and water 2 times, respectively, and then soaked in water for 24 hours.
And 5: adding a certain amount of tetraethylenepentamine into an ethanol/water (1: 4, v/v) mixed solution to prepare a 0.1 mol/L aqueous solution, and adjusting the pH value to 10-11. The cornea contact lens prepared in the step 4 was put into the amino acid aqueous solution, and then placed in an incubator and reacted at 50 ℃ for 24 hours. After the reaction is finished, taking out the corneal contact lens, washing the pH value of the solution to 7 by using distilled water, and soaking and storing for later use. Subsequently, lactobionic acid (50 mg) and NHS (24 mg) were dissolved in 7.5 mL ethanol and 7.5 mL water and reacted on a magnetic stirrer for 4 h. EDC (48 mg) and a piece of the contact lens prepared above were added to the beaker and reacted at room temperature for 24 h. After the reaction is completed. The surface lactobionic acid modified cornea contact lens is obtained by washing with ethanol and water respectively for 2 times and then soaking in water for 24 h to remove unreacted compounds.
Example 4
Corneal contact lens equilibrium water content determination
The contact lenses prepared in example 1, example 2 or example 3 were immersed in distilled water for 24 h. The surface was blotted dry with filter paper and weighed, denoted as Wswell. Then putting the mixture into a 50-DEG C oven for 24 hours, and drying the mixture to obtain the dry state mass Wdry. The equilibrium water content of the corneal contact lens is calculated according to formula 1, measured 3 times, and the average value is taken. The results show that the equilibrium water content of the corneal contact lens in example 1, example 2 or example 3 is 59.35 +/-2.98, 55.09 +/-3.14 and 52.07 +/-4.32 respectively, and the corneal contact lens obtained after amino acid grafting is more favorable for absorbing and storing water due to the hydrophilic effect of the amino acid and has higher equilibrium water content.
EWC＝[（Ｗswell -Ｗdry）／Ｗswell]X 100% (equation 1).
Example 5
The contact lenses prepared in example 1, example 2, example 3 or example 3 were soaked in PBS buffer solution containing bovine serum albumin (5 mg/mL) for 12 h. Subsequently, the non-deposited protein was removed by continuous washing with PBS buffer solution. The contact lenses were immersed in 2 mL of Sodium Dodecyl Sulfate (SDS) PBS solution and incubated at 37 ℃ for 120 min with shaking at 100 rpm in a constant temperature shaking incubator to elute proteins adsorbed on the surface. Subsequently, the eluted protein content was determined using the BCA protein concentration assay kit. Adding standard substance into wells of 96-well plate at ratio of 0, 1, 2, 4, 8, 12, 16, 20. mu.L, and adding standard substance diluent to make up to 20. mu.L. A further 20. mu.L of sample was added to a 96-well plate. 200 μ L of BCA working solution was added to each well, and left at 37 ℃ for 20-30 min. Measurement of A with microplate reader562And (4) absorbance. Individual samples were measured in triplicate and averaged. And calculating the protein concentration and the protein amount absorbed by the sample according to the standard curve.
As can be seen from the results in FIG. 6, the adsorption amounts of BSA protein in the control p-HEMA group, and the cornea contact lenses of example 1, example 2 and example 3 were 0.121. + -. 0.021, 0.067. + -. 0.014, 0.072. + -. 0.026 and 0.061. + -. 0.018 mg/tablet, respectively. The adsorption capacity of the cornea contact lens modified by the three groups of representative polymer brushes to protein is lower than that of a reference, which shows that the prepared cornea contact lens can effectively reduce the protein adsorption capacity after being modified by the polymer brushes, and is favorable for reducing the incidence rate of eye diseases after being worn for a long time.
Claims (8)
1. A preparation method of a surface polymer brush modified hydrogel material is characterized by comprising the following steps:
step 1: preparation of hydrogel material: uniformly mixing a monomer m, a monomer n, an initiator and a cross-linking agent according to a certain proportion, putting the mixture into a mold, putting the mold into an ultraviolet curing reactor or an oven for reaction for 0.2-8 h, and demolding to obtain a hydrogel material A;
step 2: activating the surface of the hydrogel material: reacting the hydrogel A with 3-aminopropyltriethoxysilane, and activating hydroxyl on the surface of the hydrogel into amino to obtain a hydrogel material B;
and step 3: preparation of polyglycidyl methacrylate PGMA: dissolving monomer glycidyl methacrylate, a chain transfer agent and an initiator into an anhydrous solvent, heating for reaction, and preparing PGMA through RAFT free radical polymerization;
and 4, step 4: grafting PGMA to the surface of the hydrogel material B through a condensation reaction between carboxyl at the end of a PGMA chain and amino on the surface of the hydrogel material B to obtain a hydrogel material C;
and 5: grafting functional molecules: dissolving the micromolecules with amino groups in the solution, adjusting the pH value of the solution to 9-12, soaking the hydrogel material C in the solution, reacting for 12-48 h, and coupling the micromolecules with amino groups to the surface of the hydrogel material C through the reaction of the amino groups of the micromolecules and epoxy groups in glycidyl ester to obtain a hydrogel material D;
in the step 1, a monomer m is methacrylic acid-beta-hydroxyethyl HEMA; the monomer N is one or two of MAA (methacrylic acid), AA (acrylic acid), acrylamide, N-vinyl pyrrolidone NVP (N-vinylpyrrolidone), 3- (triethoxysilyl) propyl methacrylate, 3-epoxypropyl tri (trimethylsiloxy) silane, N-hydroxymethyl acrylamide, N-hydroxyethyl acrylamide and 2-hydroxypropyl methacrylate;
in the step 5, the small molecule with amino groups comprises alanine, glycine, serine, lysine, arginine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, methionine, threonine, cysteine, tyrosine, asparagine, glutamine, aspartic acid or glutamic acid.
2. A preparation method of a surface polymer brush modified hydrogel material is characterized in that,
step 1: preparation of hydrogel material: uniformly mixing a monomer m, a monomer n, an initiator and a cross-linking agent according to a certain proportion, putting the mixture into a mold, putting the mold into an ultraviolet curing reactor or an oven for reaction for 0.2-8 h, and demolding to obtain a hydrogel material A;
step 2: activating the surface of the hydrogel material: reacting the hydrogel A with 3-aminopropyltriethoxysilane, and activating hydroxyl on the surface of the hydrogel into amino to obtain a hydrogel material B;
and step 3: preparation of polyglycidyl methacrylate PGMA: dissolving monomer glycidyl methacrylate, a chain transfer agent and an initiator into an anhydrous solvent, heating for reaction, and preparing PGMA through RAFT free radical polymerization;
and 4, step 4: grafting PGMA to the surface of the hydrogel material B through a condensation reaction between carboxyl at the end of a PGMA chain and amino on the surface of the hydrogel material B to obtain a hydrogel material C;
and 5: grafting functional molecules: dissolving triethylene tetramine or tetraethylene pentamine in the solution, adjusting the pH value of the solution to 9-12, soaking the hydrogel material C in the solution, reacting for 12-48 h, and reacting with lactobionic acid to obtain a hydrogel material E.
3. The preparation method of the surface polymer brush modified hydrogel material according to claim 2, wherein in the step 5, the reaction with lactose is specifically carried out by dissolving lactobionic acid and N-hydroxysuccinimide NHS in ethanol solution, placing on a magnetic stirrer for reaction for 4 h, adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride EDC and triethylene tetramine or tetraethylene pentamine solution for soaking hydrogel material C, reacting at room temperature, and washing to remove unreacted compounds after the reaction is finished, thereby obtaining the surface lactobionic acid modified corneal contact lens.
4. The method for preparing a surface polymer brush-modified hydrogel material according to claim 1 or 2, wherein,
the cross-linking agent in the step 1 is one or two of ethylene glycol dimethacrylate EGDMA, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, polyethylene glycol dimethacrylate, ethylene glycol diacrylate, diethylene glycol diacrylate, triethylene glycol diacrylate, polyethylene glycol diacrylate, glyceryl trimethacrylate, glyceryl triacrylate, glyceryl dimethacrylate and glyceryl diacrylate;
the initiator in the step 1 is one of azo compound initiator, peroxide initiator or ultraviolet initiator.
5. The method for preparing a surface polymer brush-modified hydrogel material according to claim 1 or 2, wherein,
in the step 3, the RAFT chain transfer agent is one of benzyl dithiobenzoate, isobutyl dithiobenzoate, trithiocarbonate, 4-cyanodithiobenzoylvaleric acid or carboxymethyl thiobenzoate;
the initiator in the step 3 is a free radical initiator and comprises azobisisobutyronitrile AIBN, azobisisoheptonitrile ABVN, benzoyl peroxide tert-butyl ester and methyl ethyl ketone peroxide.
6. The method for preparing a surface polymer brush-modified hydrogel material according to claim 1 or 2, wherein,
in the step 4, the condensation reaction adopts one or two of 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride EDC, N-hydroxysuccinimide NHS and dicyclohexylcarbodiimide DCC as a catalyst, and the reaction time is 12-48 h.
7. A surface polymer brush-modified hydrogel material prepared by the preparation method according to any one of claims 1 to 6.
8. Use of a surface polymer brush modified hydrogel material of claim 7 as a corneal contact lens.
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