CN112616773B - Production method for improving oxidation resistance component in embryo egg - Google Patents
Production method for improving oxidation resistance component in embryo egg Download PDFInfo
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- CN112616773B CN112616773B CN202011445950.7A CN202011445950A CN112616773B CN 112616773 B CN112616773 B CN 112616773B CN 202011445950 A CN202011445950 A CN 202011445950A CN 112616773 B CN112616773 B CN 112616773B
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 119
- 230000003647 oxidation Effects 0.000 title claims abstract description 28
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 28
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 235000013601 eggs Nutrition 0.000 claims abstract description 137
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000001301 oxygen Substances 0.000 claims abstract description 46
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 46
- 238000003780 insertion Methods 0.000 claims abstract description 27
- 230000037431 insertion Effects 0.000 claims abstract description 27
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 24
- 229940088710 antibiotic agent Drugs 0.000 claims abstract description 16
- 210000003278 egg shell Anatomy 0.000 claims abstract description 13
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 12
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 12
- 230000028327 secretion Effects 0.000 claims abstract description 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 11
- 108091005804 Peptidases Proteins 0.000 claims abstract description 9
- 102000035195 Peptidases Human genes 0.000 claims abstract description 9
- 239000004365 Protease Substances 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims abstract description 9
- 229920001184 polypeptide Polymers 0.000 claims abstract description 7
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 7
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 6
- 230000001737 promoting effect Effects 0.000 claims abstract description 6
- 230000002378 acidificating effect Effects 0.000 claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims description 45
- 238000003860 storage Methods 0.000 claims description 41
- 239000003963 antioxidant agent Substances 0.000 claims description 36
- 230000003078 antioxidant effect Effects 0.000 claims description 33
- 230000003115 biocidal effect Effects 0.000 claims description 25
- 239000007789 gas Substances 0.000 claims description 23
- 238000002347 injection Methods 0.000 claims description 18
- 239000007924 injection Substances 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 18
- 239000000126 substance Substances 0.000 claims description 16
- 239000007921 spray Substances 0.000 claims description 12
- 238000004080 punching Methods 0.000 claims description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 230000012447 hatching Effects 0.000 claims description 9
- 108091005508 Acid proteases Proteins 0.000 claims description 7
- 108090000145 Bacillolysin Proteins 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 230000002401 inhibitory effect Effects 0.000 claims description 5
- 101800000068 Antioxidant peptide Proteins 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 1
- 235000006708 antioxidants Nutrition 0.000 description 30
- 230000003064 anti-oxidating effect Effects 0.000 description 5
- 230000002349 favourable effect Effects 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- -1 vitamin E Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K41/00—Incubators for poultry
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a production method for improving oxidation resistance components in embryo eggs, which utilizes the inertia movement law of life activities, and the radian formed by a plurality of rows of insertion pipes is the same as the radian formed by the outer surface of the embryo eggs, so that the density of holes drilled on the upper surface of the embryo eggs is the same, the interior of the embryo eggs is uniformly contacted with the outside, the force applied to the embryo egg shells is uniformly distributed, the breakage of the embryo egg shells is prevented, and simultaneously the injected antibiotics are uniformly contacted with the interior of the embryo eggs, so that the fertilized eggs begin to secrete and produce a large number of various proteases, oxidation resistance polypeptides and enzymolysis products due to the influence of the outside environment, and then the created oxygen enrichment and antibiotics inhibit the environment to inhibit the secretion of acidic proteases and neutral proteases, thereby promoting the growth of alkaline proteases, greatly improving the oxidation resistance components in the embryo eggs, and having better creativity and practicability.
Description
Technical Field
The invention relates to the field of biology, in particular to the field of production methods for improving oxidation resistance components in embryo eggs.
Background
Antioxidants are widely used in the fields of foods, health products, daily chemicals, and the like. Antioxidants on the one hand can prevent the above products from changing colour or deteriorating due to oxidation; on the other hand, some oxidizing agents play active roles even in related products, such as anti-oxidation and free radical scavenging effects, and in recent years, natural and safe antioxidants are actively searched for, and various anti-oxidation compounds such as vitamin E, tea polyphenol and the like are found in many natural animal and plant materials, but most of the existing raw materials for preparing the anti-oxidation peptide are substances with less reserves in the environment, and then the anti-oxidation compounds are prepared by utilizing objects with more reserves in the environment to reduce the production cost of the anti-oxidation peptide.
The fertilized egg can develop embryo at a specific temperature, the embryo is in a high-concentration oxygen environment and is not damaged by oxidation, the biological phenomenon shows that some oxidation-resistant substances in the embryo egg can protect the embryo from normal development, during hatching, the protein in the embryo egg is converted into nutrient substances which can be absorbed by the embryo and must be degraded into low-molecular-weight peptide or amino acid, which provides favorable conditions for preparing the oxidation-resistant active peptide from the embryo egg, but the content of oxidation-resistant components in the embryo egg is still low, even if the extraction has larger production cost, the content of the oxidation-resistant active components in the embryo egg is improved, and no suitable solution exists in the prior art, so that a production method for improving the oxidation-resistant components in the embryo egg is provided.
Disclosure of Invention
The invention mainly aims to provide a production method for improving oxidation resistance components in embryo eggs, which can effectively solve the problems in the background technology: the content of antioxidant components in embryo eggs is still low, even if the extraction has a large production cost, no suitable solution exists in the prior art how to increase the content of antioxidant active components in embryo eggs.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: a production method for improving oxidation resistance components in embryo eggs, which comprises the following steps:
step one: cleaning fertilized eggs which are just born, vertically placing the fertilized eggs in the fixing grooves on the upper surface of a storage disc, placing one fertilized egg in each fixing groove, movably inserting a slot on the side surface of the storage disc into a sliding plate on the inner surface of a culture cabin, placing the storage disc into the culture cabin, culturing the embryo eggs in the incubator for 7-8 days under the conditions of constant temperature and humidity and constant oxygen concentration, and carrying out the second step;
step two: after the step one is completed, the control screen controls the air cylinder to be integrally pressed downwards, the lantern ring slides on the outer surface of the guide rail in the chute, the spray head drives the insertion pipes to drill holes on the upper surface of the fertilized egg, the plurality of rows of insertion pipes are distributed in a certain radian, the radian formed by the plurality of rows of insertion pipes is the same as the radian formed by the outer surface of the embryo egg, so that the density of the holes drilled on the upper surface of the embryo egg is the same, the interior of the embryo egg is uniformly contacted with the outside, the force applied to the embryo eggshell is uniformly distributed, and the breakage of the embryo eggshell is prevented;
step three: the fertilized egg begins to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the external environment, so as to resist the influence of the external environment;
step four: the first control screen controls the cylinder to be integrally pressed down again, the lantern ring slides on the outer surface of the guide rail in the chute, and antibiotics in the antibiotic storage box are injected into the fertilized eggs through the antibiotic output pipe, the feeding pipe and the injection port and then inserted into the fertilized eggs through the insertion pipe so as to generate antioxidant components;
step five: after the step four is completed, the display screen II controls the reaction in the reaction kettle to be rapidly carried out, a large amount of oxygen enters the inside of the culture cabin through the gas filter, the flow valve and the oxygen inlet, so that the oxygen concentration in the culture cabin reaches ninety percent, and a large amount of embryo eggs generate antioxidant components to resist the oxidation, and after one-day oxygen enrichment supply is carried out, the oxygen concentration in the culture cabin reaches the normal level of the external air;
step six: and step five, continuously reducing the oxygen concentration in the culture cabin, and generating a large amount of antioxidant substances due to the biological inertia effect, wherein the content of antioxidant components in the embryo eggs is the highest, and the embryo eggs can be taken out from the culture cabin for antioxidant peptide extraction.
The invention further improves the production device for improving the oxidation resistance component in the embryo egg, the device comprises an incubator and a reaction kettle, the reaction kettle is fixedly arranged on the upper surface of the incubator, the back side of the incubator is fixedly provided with an antibiotic storage box, antibiotics in the antibiotic storage box have chemical characteristics of inhibiting the growth of acidic and neutral proteases, the interior of the incubator is provided with a culture cabin, the middle part of the incubator is provided with a storage disc, the interior of the culture cabin is provided with a punching mechanism which is positioned right above the storage disc, and the output end of the reaction kettle is connected with a flow controller through a gas transmission pipeline.
The invention is further improved in that the flow controller is suitable for controlling the flow of oxygen to flow out so as to control the reaction degree in the reaction kettle, the output end of the reaction kettle is connected with a gas filter through a pipeline, the gas filter is suitable for filtering gas generated by reaction in the reaction kettle so as to prevent other gas generated in the reaction kettle from generating side effects on the growth of embryo eggs, the output end of the gas filter is connected with the culture cabin through a pipeline, the right side of the culture box is fixedly provided with a first control screen, the first control screen controls the environment in the culture box through a plc system, the right side of the culture box is provided with a feeding pipe below the first control screen, the inner surface of the culture box is provided with a sliding groove, the side surface of the culture cabin is fixedly provided with a sliding plate, the inside of the sliding groove is fixedly provided with a guide rail, the inside of the culture cabin is provided with an oxygen inlet which is uniformly distributed on the inner surface of the culture cabin, the upper surface of the culture cabin is fixedly provided with a cylinder, the output end of the cylinder is fixedly connected with the upper surface of a chiseling mechanism, and the first control screen is electrically connected with the first control screen.
The invention is further improved in that a second display screen is fixedly arranged on the outer surface of the reaction kettle, the second display screen is suitable for controlling and accurately controlling the reaction degree in the reaction kettle, an upper cover is covered on the upper surface of the reaction kettle, a first raw material pipe and a second raw material pipe are arranged on the upper surface of the upper cover, and the first raw material pipe and the second raw material pipe are suitable for adding raw materials for manufacturing oxygen.
The invention is further improved in that an antibiotic output pipe is fixedly arranged at the output end of the antibiotic storage box, inserting grooves are formed in two sides of the storage disc, the inner surface of each inserting groove is connected with the sliding plate in an inserting mode, a fixing groove is formed in the upper surface of the storage disc, a protection pad is fixedly arranged on the inner surface of each fixing groove, the diameter of each fixing groove is matched with the transverse diameter of an embryo egg, the embryo eggs are conveniently taken out and vertically fixed, the punching mechanism comprises a connecting plate, a spray head is fixedly arranged on the lower surface of the connecting plate, a plurality of rows of inserting pipes are fixedly arranged on the lower surface of the spray head, the plurality of rows of inserting pipes are arranged in a certain radian mode, the radian formed by the plurality of rows of inserting pipes is identical to the radian formed by the outer surface of the embryo eggs, the density of punching holes formed in the upper surface of the embryo eggs is identical, the inner portion of the embryo eggs is uniformly contacted with the outside, the force applied to the embryo eggs is uniformly distributed, the embryo eggs are prevented from cracking, a lantern ring is fixedly arranged on the back side of the connecting plate, the inner surface of the lantern ring is movably sleeved with the guide rail, the upper surface of the connecting plate is fixedly connected with the air cylinder, the right side of the connecting plate is provided with the connecting plate, the injection port is fixedly connected with the injection port, and the injection port is stably runs through the injection port, and can be connected with the injection port.
The invention is further improved in that the best hatching condition of the fertilized egg is that the fertilized egg is placed in an incubator with the hatching temperature of 38-39 ℃, the oxygen concentration of 40-50% and the relative humidity of 50-55% for hatching for 6-7d, so that the synthesis and secretion of antioxidant substances in the embryo egg are facilitated.
The invention is further improved in that the antibiotic contains a large amount of components for inhibiting the secretion of the acid protease and the neutral protease so as to inhibit the secretion of the acid protease and the neutral protease, thereby promoting the growth of the alkaline protease.
Compared with the prior art, the invention has the following beneficial effects:
compared with the prior art, the invention utilizes the inertia movement law of life movements, the radian formed by a plurality of rows of insertion pipes is the same as the radian formed by the outer surface of the embryo egg, so that the density of holes drilled on the upper surface of the embryo egg is the same, the interior of the embryo egg is uniformly contacted with the outside, the force applied to the embryo egg is uniformly distributed, the breakage of the embryo egg is prevented, and simultaneously the injected antibiotics are uniformly contacted with the interior of the embryo egg, so that the fertilized egg starts to secrete and produce a large number of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the outside environment, and then the created antibiotics inhibit the environment to inhibit the secretion of acid proteases and neutral proteases, thereby promoting the growth of alkaline proteases, the created oxygen-enriched environment is favorable for the generation of antioxidant active ingredient substances, and the antioxidant ingredients in the embryo egg can be greatly improved, and the invention has better creativity and practicability.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a production device for improving the oxidation resistance component in embryo eggs.
FIG. 2 is a schematic view showing the internal structure of an incubator of a production apparatus for improving oxidation resistance components in embryo eggs according to the present invention.
Fig. 3 is a schematic structural view of a punching mechanism of a production device for improving oxidation resistance components in embryo eggs.
Fig. 4 is a schematic view of a spray head of a production apparatus for enhancing antioxidant ingredients in embryonated eggs according to the present invention.
FIG. 5 is a schematic flow chart of a method for improving the antioxidant component of embryo eggs according to the present invention.
In the figure: 1. an incubator; 2. a reaction kettle; 3. an antibiotic storage tank; 4. a culture cabin; 5. a storage tray; 7. a punching mechanism; 8. a flow controller; 9. a gas filter; 10. a flow valve; 101. a first control screen; 102. a feeding pipe; 103. a chute; 104. a slide plate; 105. a guide rail; 106. an oxygen inlet; 107. a cylinder; 201. a second display screen; 202. an upper cover; 203. a first raw material pipe; 204. a second raw material pipe; 301. antibiotic output tube; 501. a slot; 502. a fixing groove; 503. a protective pad; 701. a spray head; 702. a cannula; 703. a collar; 704. an injection port.
Detailed Description
In order that the technical means, the creation characteristics, the achievement of the objects and the effects of the present invention may be easily understood, it should be noted that in the description of the present invention, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "a", "an", "the" and "the" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. The invention is further described below in conjunction with the detailed description.
Example 1
As shown in figures 1-4, the production method for improving the oxidation resistance component in the embryo egg comprises a production device for improving the oxidation resistance component in the embryo egg, the device comprises an incubator (1) and a reaction kettle (2), the reaction kettle (2) is fixedly arranged on the upper surface of the incubator (1), an antibiotic storage box (3) is fixedly arranged on the back side of the incubator (1), antibiotics in the antibiotic storage box (3) have chemical characteristics for inhibiting the growth of acidic and neutral proteases, a culture cabin (4) is arranged in the incubator (1), a storage disc (5) is arranged in the middle of the incubator (1), a punching mechanism (7) is arranged right above the storage disc (5) in the culture cabin (4), and the output end of the reaction kettle (2) is connected with a flow controller (8) through a gas transmission pipeline.
The flow controller (8) is suitable for controlling the flow of oxygen to flow out so as to control the reaction degree in the reaction kettle (2), the output end of the reaction kettle (2) is connected with a gas filter (9) through a pipeline, the gas filter (9) is suitable for filtering gas generated by reaction in the reaction kettle (2) so as to prevent other gas generated in the reaction kettle (2) from generating side effects on the growth of embryo eggs, the output end of the gas filter (9) is connected with a culture cabin (4) through a pipeline, a first control screen (101) is fixedly arranged on the right side of the culture box (1), the first control screen (101) controls the environment in the culture box (1) through a plc system, an inlet pipe (102) is arranged on the right side of the culture box (1) and below the first control screen (101), a slide groove (103) is formed in the inner surface of the culture box (1), a slide plate (104) is fixedly arranged on the side surface of the culture cabin (4), a guide rail (105) is fixedly arranged in the inner side of the slide groove (103), an oxygen inlet (106) is formed in the upper part of the slide plate (104), an oxygen inlet (106) is uniformly distributed on the surface of the culture cabin (4) and is fixedly connected with a surface of a cylinder (107) on the surface of the culture cabin (7), the electrical input end of the cylinder (107) is electrically connected with the first control screen (101).
The surface fixed mounting of reation kettle (2) has No. two display screens (201), and No. two display screens (201) are suitable for the inside reaction degree of control accurate control reation kettle (2), and the upper surface lid of reation kettle (2) has upper cover (202), and the upper surface mounting of upper cover (202) has raw materials pipe (203) and No. two raw materials pipe (204), and raw materials pipe (203) and No. two raw materials pipe (204) are suitable for adding the raw materials of making oxygen.
The antibiotic storage box (3) is fixedly provided with an antibiotic output pipe (301) at the output end, two sides of the storage disc (5) are provided with slots (501), the inner surface of each slot (501) is spliced with the sliding plate (104), the upper surface of the storage disc (5) is provided with a fixed slot (502), the inner surface of each fixed slot (502) is fixedly provided with a protection pad (503), the diameter of each fixed slot (502) is matched with the transverse diameter of an embryo egg, thus the embryo egg can be conveniently taken out and vertically fixed, the punching mechanism (7) comprises a connecting plate, the lower surface of the connecting plate is fixedly provided with a spray head (701), the lower surface of the spray head (701) is fixedly provided with a plurality of rows of insertion pipes (702), the rows of insertion pipes (702) are distributed in a certain radian, the radian formed by the multiple rows of the insertion pipes (702) is the same as the radian formed by the outer surfaces of the embryo eggs, so that the density of the holes drilled on the upper surfaces of the embryo eggs is the same, the interior of the embryo eggs is in uniform contact with the outside, the force applied to the embryo eggs is uniformly distributed, the breakage of the embryo eggs is prevented, the back side of the connecting plate is fixedly provided with a lantern ring (703), the inner surface of the lantern ring (703) is movably sleeved with the guide rail (105), the upper surface of the connecting plate is fixedly connected with the air cylinder (107), the right side of the connecting plate is provided with an injection port (704), the output end of the injection port (704) is communicated with the insertion pipe (702), the input end of the injection port (704) is communicated with the feeding pipe (102), and under the pushing of the air cylinder (107), the punching mechanism (7) can stably and vertically lift to punch holes and evenly inject embryo eggs.
This embodiment can realize: the radian formed by the multiple rows of the insertion pipes (702) is the same as the radian formed by the outer surfaces of the embryo eggs, so that the density of the holes drilled on the upper surfaces of the embryo eggs is the same, the interior of the embryo eggs is uniformly contacted with the outside, the force applied to the embryo egg shells is uniformly distributed, the breakage of the embryo egg shells is prevented, and the injected antibiotics are uniformly contacted with the interior of the embryo eggs.
Example 2
As shown in fig. 1 to 5, a method for improving the oxidation resistance component in embryo eggs comprises the following steps:
step one: cleaning fertilized eggs which are just born, vertically placing the fertilized eggs in the fixing grooves (502) on the upper surface of the storage tray (5), placing one fertilized egg in each fixing groove (502), movably inserting a slot (501) on the side surface of the storage tray (5) into a sliding plate (104) on the inner surface of the culture cabin (4), placing the storage tray (5) into the culture cabin (4), and culturing the embryo eggs in the incubator (1) for 7-8 days under the conditions of constant temperature and humidity and constant oxygen concentration;
step two: after the step one is completed, the control screen (101) controls the air cylinder (107) to be integrally pressed down, the lantern ring (703) slides on the outer surface of the guide rail (105) in the chute (103), the spray head (701) drives the insertion pipes (702) to drill holes on the upper surface of the fertilized egg, the multiple rows of insertion pipes (702) are distributed in a certain radian, the radian formed by the multiple rows of insertion pipes (702) is the same as the radian formed by the outer surface of the embryo egg, so that the density of the holes drilled on the upper surface of the embryo egg is the same, the interior of the embryo egg is uniformly contacted with the outside, and the force applied to the embryo eggshell is uniformly distributed, so that the embryo eggshell is prevented from cracking;
step three: the fertilized egg begins to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the external environment, so as to resist the influence of the external environment;
step four: the first control screen (101) controls the cylinder (107) to be integrally pressed down again, the lantern ring (703) slides on the outer surface of the guide rail (105) in the chute (103), and antibiotics in the antibiotic storage box (3) are injected into fertilized eggs through the antibiotic output pipe (301), the feeding pipe (102) and the injection port (704) and through the insertion pipe (702) to generate antioxidant components;
step five: after the step four is completed, a second display screen (201) controls the reaction inside the reaction kettle (2) to be rapidly carried out, a large amount of oxygen enters the inside of the culture cabin (4) through a gas filter (9), a flow valve (10) and an oxygen inlet (106), so that the oxygen concentration inside the culture cabin (4) reaches ninety percent, and thus, a large amount of antioxidant components are generated by embryo eggs to resist the oxidation, and after one day of oxygen-enriched supply is carried out, the oxygen concentration inside the culture cabin (4) reaches the normal level of the external air;
step six: and step five, continuously reducing the oxygen concentration in the culture cabin (4), and generating a large amount of antioxidant substances due to the biological inertia effect, wherein the content of antioxidant components in the embryo eggs is the highest, and taking the embryo eggs out of the culture cabin (4) for extraction of antioxidant peptides.
The optimal hatching condition of the fertilized egg is that the fertilized egg is placed in an incubator with the hatching temperature of 38-39 ℃, the oxygen concentration of 40-50% and the relative humidity of 50-55% for 6-7d, so that the synthesis and secretion of antioxidant substances in the embryo egg are facilitated.
Antibiotics contain a large amount of components that inhibit the secretion of acid protease and neutral protease to inhibit the secretion of acid protease and neutral protease, thereby promoting the growth of alkaline protease.
This embodiment can realize: the invention utilizes the inertia movement law of life movements, the radian formed by a plurality of rows of insertion pipes (702) is the same as the radian formed by the outer surface of an embryo egg, so that the density of holes drilled on the upper surface of the embryo egg is the same, the interior of the embryo egg is uniformly contacted with the outside, the force applied to the embryo egg is uniformly distributed, the embryo egg shell is prevented from being broken, and simultaneously the injected antibiotics are uniformly contacted with the interior of the embryo egg, so that the fertilized egg starts to secrete and produce a large number of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the outside environment, and then the created antibiotics inhibit the environment to inhibit the secretion of acidic proteases and neutral proteases, thereby promoting the growth of alkaline proteases, and the created oxygen-enriched environment is favorable for the generation of antioxidant active ingredient substances, so that the antioxidant ingredient in the embryo egg can be greatly improved.
In the production method for improving the oxidation resistance component in the embryo eggs, firstly, the fertilized eggs just born are cleaned, vertically placed in the fixing grooves (502) on the upper surface of the storage tray (5), one fertilized egg is placed in each fixing groove (502), a slot (501) on the side surface of the storage tray (5) is movably inserted into a sliding plate (104) on the inner surface of the culture cabin (4), the storage tray (5) is placed in the culture cabin (4), after the embryo eggs are cultured for 7-8 days under the conditions of constant temperature and constant humidity in the incubator (1) and constant oxygen concentration, a first control screen (101) controls a cylinder (107) to integrally press down, a lantern ring (703) slides on the outer surface of a guide rail (105) in the sliding groove (103), the spray head (701) drives the insertion pipes (702) to drill holes on the upper surface of the fertilized egg, the plurality of rows of insertion pipes (702) are distributed in a certain radian, the radian formed by the plurality of rows of insertion pipes (702) is the same as the radian formed by the outer surface of the embryo egg, so that the density of the holes drilled on the upper surface of the embryo egg is the same, the interior of the embryo egg is uniformly contacted with the outside, the force applied to the embryo eggshell is uniformly distributed, the embryo eggshell is prevented from being broken, the fertilized egg begins to secrete and produce a large number of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the outside environment, the influence of the outside environment is resisted, the first control screen (101) controls the cylinder (107) to be integrally pressed down again, the sleeve ring (703) slides on the outer surface of the guide rail (105) in the chute (103), antibiotics in the antibiotic storage tank (3) are injected into fertilized eggs through the antibiotic output pipe (301), the feed pipe (102) and the injection port (704) and through the insertion pipe (702) so as to generate antioxidant components, the second display screen (201) controls the reaction in the reaction kettle (2) to rapidly perform, a large amount of oxygen enters the culture cabin (4) through the gas filter (9), the flow valve (10) and the oxygen inlet (106), so that the oxygen concentration in the culture cabin (4) reaches ninety percent, and thus, the antioxidant components are generated in a large amount for resisting oxidation, the oxygen concentration in the culture cabin (4) reaches the normal level of the outside air after one day of oxygen-enriched supply, the oxygen concentration in the culture cabin (4) is continuously reduced, a large amount of antioxidant substances are generated due to biological inertia effect, and at the moment, the content of the antioxidant components in the embryo eggs is the highest, and the embryo eggs can be taken out from the culture cabin (4) to extract antioxidant peptides.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. The utility model provides an improve apparatus for producing of oxidation resistance composition in embryo egg which characterized in that: the device comprises an incubator and a reaction kettle, wherein the reaction kettle is fixedly arranged on the upper surface of the incubator, an antibiotic storage box is fixedly arranged on the back side of the incubator, antibiotics in the antibiotic storage box have chemical characteristics of inhibiting the growth of acidic and neutral proteases, a culture cabin is arranged in the incubator, a storage disc is arranged in the middle of the incubator, a punching mechanism is arranged in the culture cabin and positioned right above the storage disc, and the output end of the reaction kettle is connected with a flow controller through a gas transmission pipeline;
the flow controller is suitable for controlling the flow of oxygen flowing out so as to control the reaction degree in the reaction kettle, the output end of the reaction kettle is connected with a gas filter through a pipeline, the gas filter is suitable for filtering gas generated by reaction in the reaction kettle so as to prevent other gases generated in the reaction kettle from generating side effects on the growth of embryo eggs, the output end of the gas filter is connected with a culture cabin through a pipeline, a first control screen is fixedly arranged on the right side of the culture box, the first control screen controls the environment in the culture box through a plc system, a feeding pipe is arranged on the right side of the culture box and below the first control screen, a sliding groove is formed in the inner surface of the culture box, a sliding plate is fixedly arranged on the side surface of the culture cabin, a guide rail is fixedly arranged in the inner side of the sliding groove, an oxygen inlet is formed in the upper surface of the culture cabin, a cylinder is fixedly arranged on the upper surface of the culture cabin, the output end of the cylinder is fixedly connected with the upper surface of a chiseling mechanism, and the first control end of the first control screen is electrically connected with the first control screen;
the external surface of the reaction kettle is fixedly provided with a second display screen, the second display screen is suitable for accurately controlling the reaction degree in the reaction kettle, the upper surface of the reaction kettle is covered with an upper cover, the upper surface of the upper cover is provided with a first raw material pipe and a second raw material pipe, and the first raw material pipe and the second raw material pipe are suitable for adding raw materials for manufacturing oxygen;
the device comprises an antibiotic storage box, an antibiotic output pipe, a storage tray, a fixing groove, a protection pad, a punching mechanism, an injection port, a guide rail, a jack mechanism and a jack mechanism, wherein the antibiotic output pipe is fixedly arranged at the output end of the antibiotic storage box, the two sides of the storage tray are provided with slots, the inner surface of each slot is connected with a sliding plate in an inserting mode, the upper surface of the storage tray is provided with a fixing groove, the inner surface of each fixing groove is fixedly provided with a protection pad, the diameter of each fixing groove is matched with the transverse diameter of an embryo egg, the embryo egg is conveniently taken out and vertically fixed, the jack mechanism comprises a connecting plate, the lower surface of the connecting plate is fixedly provided with a spray head, the lower surface of the spray head is fixedly provided with a plurality of rows of insertion pipes, the insertion pipes are distributed in a certain radian, the radian of the rows of the insertion pipes is the same as the radian of the outer surface of the embryo egg, the density of the punching holes of the upper surface of the embryo egg is the same, the inside of the embryo egg is uniformly contacted with the outside, the force applied to the embryo egg is uniformly distributed, the embryo egg is prevented from being broken, the back side of the connecting plate is fixedly provided with a lantern ring, the inner surface of the lantern ring is movably arranged with the embryo egg, the upper surface of the connecting plate is fixedly connected with the air cylinder, the injection port is fixedly arranged on the right side of the injection port, the injection port is arranged on the inlet, and the inlet is pushed by the injection port and can be connected with the jack through the jack mechanism;
the optimal hatching condition of the fertilized egg is that the fertilized egg is placed in an incubator with the hatching temperature of 38-39 ℃, the oxygen concentration of 40-50% and the relative humidity of 50-55% for hatching for 6-7d, so that the synthesis and secretion of antioxidant substances in the embryo egg are facilitated;
the antibiotics contain a large amount of components for inhibiting the secretion of acid protease and neutral protease so as to inhibit the secretion of acid protease and neutral protease, thereby promoting the growth of alkaline protease;
the method comprises the following steps:
step one: cleaning fertilized eggs which are just born, vertically placing the fertilized eggs in the fixing grooves on the upper surface of a storage disc, placing one fertilized egg in each fixing groove, movably inserting a slot on the side surface of the storage disc into a sliding plate on the inner surface of a culture cabin, placing the storage disc into the culture cabin, culturing the embryo eggs in the incubator for 7-8 days under the conditions of constant temperature and humidity and constant oxygen concentration, and carrying out the second step;
step two: after the step one is completed, the control screen controls the air cylinder to be integrally pressed downwards, the lantern ring slides on the outer surface of the guide rail in the chute, the spray head drives the insertion pipes to drill holes on the upper surface of the fertilized egg, the plurality of rows of insertion pipes are distributed in a certain radian, the radian formed by the plurality of rows of insertion pipes is the same as the radian formed by the outer surface of the embryo egg, so that the density of the holes drilled on the upper surface of the embryo egg is the same, the interior of the embryo egg is uniformly contacted with the outside, the force applied to the embryo eggshell is uniformly distributed, and the breakage of the embryo eggshell is prevented;
step three: the fertilized egg begins to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the external environment, so as to resist the influence of the external environment;
step four: the first control screen controls the cylinder to be integrally pressed down again, the lantern ring slides on the outer surface of the guide rail in the chute, and antibiotics in the antibiotic storage box are injected into the fertilized eggs through the antibiotic output pipe, the feeding pipe and the injection port and then inserted into the fertilized eggs through the insertion pipe so as to generate antioxidant components;
step five: after the step four is completed, the display screen II controls the reaction in the reaction kettle to be rapidly carried out, a large amount of oxygen enters the inside of the culture cabin through the gas filter, the flow valve and the oxygen inlet, so that the oxygen concentration in the culture cabin reaches ninety percent, and a large amount of embryo eggs generate antioxidant components to resist the oxidation, and after one-day oxygen enrichment supply is carried out, the oxygen concentration in the culture cabin reaches the normal level of the external air;
step six: and step five, continuously reducing the oxygen concentration in the culture cabin, and generating a large amount of antioxidant substances due to the biological inertia effect, wherein the content of antioxidant components in the embryo eggs is the highest, and the embryo eggs can be taken out from the culture cabin for antioxidant peptide extraction.
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