CN112578113A - Abietic acid colloidal gold detection card and manufacturing method thereof - Google Patents
Abietic acid colloidal gold detection card and manufacturing method thereof Download PDFInfo
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- CN112578113A CN112578113A CN202010999628.2A CN202010999628A CN112578113A CN 112578113 A CN112578113 A CN 112578113A CN 202010999628 A CN202010999628 A CN 202010999628A CN 112578113 A CN112578113 A CN 112578113A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Abstract
The invention discloses a rosin acid colloidal gold detection card and a manufacturing method thereof, and the rosin acid colloidal gold detection card comprises a bottom plate, a sample pad, an immune colloidal gold combination pad, an immune nitrocellulose membrane and a water absorption pad, wherein the sample pad, the immune colloidal gold combination pad, the immune nitrocellulose membrane and the water absorption pad are sequentially connected end to end and fixed on the surface of the bottom plate, the immune colloidal gold combination pad contains a colloidal gold-labeled anti-rosin acid monoclonal antibody, and the immune nitrocellulose membrane is coated with a detection line of a rosin acid-bovine serum albumin complex and a quality control line of goat anti-rabbit IgG. The method is simple and convenient to operate, has high sensitivity, can obtain the detection result in a short time, conveniently, quickly and accurately detects the content of the abietic acid in the medicine or food, and provides convenience for field detection.
Description
Technical Field
The invention belongs to the field of safety detection of traditional Chinese medicinal materials, traditional Chinese medicine decoction pieces and traditional Chinese medicine preparations, relates to a detection method of illegal additives in the traditional Chinese medicinal materials, the traditional Chinese medicine decoction pieces and the traditional Chinese medicine preparations, and particularly relates to a rosin acid colloidal gold detection card and a manufacturing method thereof.
Background
One of the main components of rosin extract of abietic acid is a tricyclic diterpene oxygen-containing compound, which is commonly used in paper making industry or plant inhibitor, etc., and has special fragrance. However, rosin acid has a certain toxicity to human body, and can cause acute poisoning symptoms such as headache, dizziness, cough, asthma and the like. In recent years, illegal merchants illegally add abietic acid into traditional Chinese medicinal materials and traditional Chinese medicine decoction pieces to be secondary and good for illegal profit, so that the abietic acid flows into Chinese patent medicines and causes harm to public health. In recent years, illegal addition of rosin acid in various medicinal materials such as frankincense, myrrh and the like and preparations thereof is found, and arrangement and market order of the medicines are seriously influenced.
At present, for the detection of illegally added abietic acid in traditional Chinese medicinal materials, a sample is mainly confirmed by adopting a high performance liquid chromatography (HPLC method), a high performance liquid chromatography-ultraviolet tandem detection (HPLC-PAD method) and a high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) secondary mass spectrum. The methods such as high performance liquid chromatography and liquid chromatography-mass spectrometry are the most advanced modern analysis means, can well separate and detect the abietic acid, and reach a very low detection limit, and the sensitivity is very high. By adopting a standard substance control method, the content of the abietic acid in the sample can be detected very accurately.
In addition, no quick detection reagent box with abietic acid is found in the market, and if the experimental method is adopted, the operation process is complicated, the experimental time is long, the experimental cost is high, and the method is not suitable for field detection.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a rosin acid colloidal gold detection card and a manufacturing method thereof.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides a rosin acid colloidal gold detection card which comprises a bottom plate, a sample pad, an immune colloidal gold combination pad, an immune nitrocellulose membrane and a water absorption pad, wherein the sample pad, the immune colloidal gold combination pad, the immune nitrocellulose membrane and the water absorption pad are sequentially connected end to end and fixed on the surface of the bottom plate, the immune colloidal gold combination pad contains a colloidal gold-labeled anti-rosin acid monoclonal antibody, and the immune nitrocellulose membrane is coated with a detection line of a rosin acid-bovine serum albumin complex and a quality control line of goat anti-rabbit IgG.
In a preferred embodiment of the present invention, the gold colloid in the immune gold conjugate pad is in a powder form, and the particles formed by the gold colloid are spherical with a diameter of 30 nm.
As a preferable technical scheme of the invention, the bottom plate is in a strip shape and is made of a PVC material, and the sample pad is made of a glass fiber material.
The invention also provides a manufacturing method of the rosin acid colloidal gold detection card, which comprises the following steps:
step one, preparing colloidal gold: preparing a colloidal gold solution, adjusting the pH value to 8-9, and then keeping for later use;
second, antibody preparation: preparing an anti-abietic acid monoclonal antibody by adopting an ascites induction method in an animal body, and purifying the obtained antibody by adopting an affinity purification method;
thirdly, adding the obtained anti-abietic acid monoclonal antibody into the colloidal gold solution obtained in the first step, stirring and mixing for 30min at the room temperature of 25-30 ℃, adding polyethylene glycol, stirring and mixing for 30min again, discarding supernatant, and precipitating to obtain a marked anti-abietic acid monoclonal antibody;
fourthly, preparing an immune colloidal gold bonding pad, using glass fiber paper as the colloidal gold bonding pad, soaking the glass fiber paper in a treatment solution with pH of 7.8 and 0.1% TritonX-100 for 3min, taking out the glass fiber paper, drying the glass fiber paper at the temperature of 35-40 ℃, diluting the colloidal gold solution prepared in the first step, putting the diluted colloidal gold solution into the glass fiber paper, soaking the colloidal gold solution for 5min, taking out the colloidal gold solution, and naturally airing the colloidal gold solution for later use;
the fifth step, prepare the sample pad, soak the sample pad in 0.01M PBS, pH7.4, 2% Triton X-100, 3% BSA, 0.07% NaN3Taking out the treatment liquid for 3min, and drying at 35-40 deg.C for use;
sixthly, spraying the abietic acid-bovine serum albumin complex on a detection line as a T line, spraying the goat anti-rabbit IgG on a quality control line as a C line, coating for 2 hours at 35-40 ℃, and naturally airing or drying at room temperature to prepare an immune nitrocellulose membrane;
and seventhly, sequentially adhering the sample pad, the colloidal gold combined pad, the immune nitrocellulose membrane and the water absorption pad on the bottom plate, cutting to obtain the reagent strip, and then assembling.
Optionally, the manufacturing step of the colloidal gold fixture in the first step is as follows: 1. taking a 300mL triangular flask, adding 100mL double distilled water and 1mL 1% gold chloride solution, filtering with a 0.22 μm filter membrane, and heating to boil; 2. filtering 4mL of 1% sodium citrate with a 0.22-micrometer filter membrane, adding into the solution, rapidly mixing uniformly, heating and keeping boiling for 10-15min, continuously stirring to make the color of the solution in the triangular flask change from light yellow, purple and wine red, taking down the triangular flask, cooling to room temperature, supplementing to the original volume with double distilled water, preparing into wine red colloidal gold solution, and filtering with a 0.22-micrometer filter membrane; 3. and (3) preserving the colloidal gold solution at 4 ℃ by using a reagent bottle for later use, and meanwhile, when floating objects exist on the surface of the colloidal gold solution, the colloidal gold solution is unstable and has poor uniformity and needs to be discarded for re-manufacture.
Optionally, the material diluted by the colloidal gold solution in the fourth step is: 7% of 1.0mTris liquid, 0.3% of polyethylene glycol 20000, 0.18% of BAS and the balance of water.
Alternatively, the concentrations of the abietic acid-bovine serum albumin complex and the goat anti-rabbit IgG in the sixth step are 2.1mg/mL and 1.2mg/mL, respectively.
Optionally, the abietic acid-bovine serum albumin complex in the sixth step is prepared by the following steps:
mixing abietic acid and carboxymethyl hydroxylamine hydrochloride, and stirring for reaction to obtain a reactant;
and mixing the reactant and bovine serum albumin, and stirring for reaction to obtain the abietic acid-bovine serum albumin compound.
Compared with the prior art, the invention has the following beneficial effects:
the method is simple and convenient to operate, has high sensitivity, can obtain the detection result in a short time, conveniently, quickly and accurately detects the content of the abietic acid in the medicine or food, and provides convenience for field detection.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a cross-sectional view of a colloidal gold test card according to the present invention;
FIG. 2 is another perspective view of FIG. 1;
FIG. 3 is a graph showing the results of the test according to the present invention;
in the figure: 1. a base plate; 2. a sample pad; 3. an immune colloidal gold conjugate pad; 4. an immunonitrocellulose membrane; 5. a water absorbent pad; 6. detecting lines; 7. and (4) quality control line.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
Example 1
As shown in figures 1-3, the invention provides a rosin acid colloidal gold detection card and a manufacturing method thereof, the rosin acid colloidal gold detection card comprises a bottom plate 1, a sample pad 2, an immune colloidal gold combination pad 3, an immune nitrocellulose membrane 4 and a water absorption pad 5, wherein the sample pad 2, the immune colloidal gold combination pad 3, the immune nitrocellulose membrane 4 and the water absorption pad 5 are sequentially connected end to end and fixed on the surface of the bottom plate 1, the immune colloidal gold combination pad 3 contains a colloidal gold-labeled anti-rosin acid monoclonal antibody, and the immune nitrocellulose membrane 4 is coated with a detection line 6 of a rosin acid-bovine serum albumin complex and a quality control line 7 of goat anti-rabbit IgG.
Further, the colloidal gold in the immune colloidal gold conjugate pad 3 is in a powder shape, and the formed particles are spherical with the diameter of 30 nm.
The bottom plate 1 is strip-shaped and made of PVC material, and the sample pad 2 is made of glass fiber material.
The invention also provides a manufacturing method of the rosin acid colloidal gold detection card, which comprises the following steps:
step one, preparing colloidal gold: preparing a colloidal gold solution, adjusting the pH value to 8-9, and then keeping for later use;
second, antibody preparation: preparing an anti-abietic acid monoclonal antibody by adopting an ascites induction method in an animal body, and purifying the obtained antibody by adopting an affinity purification method;
thirdly, adding the obtained anti-abietic acid monoclonal antibody into the colloidal gold solution obtained in the first step, stirring and mixing for 30min at the room temperature of 25-30 ℃, adding polyethylene glycol, stirring and mixing for 30min again, discarding supernatant, and precipitating to obtain a marked anti-abietic acid monoclonal antibody;
fourthly, preparing an immune colloidal gold bonding pad, using glass fiber paper as the colloidal gold bonding pad, soaking the glass fiber paper in a treatment solution with pH of 7.8 and 0.1% TritonX-100 for 3min, taking out the glass fiber paper, drying the glass fiber paper at the temperature of 35-40 ℃, diluting the colloidal gold solution prepared in the first step, putting the diluted colloidal gold solution into the glass fiber paper, soaking the colloidal gold solution for 5min, taking out the colloidal gold solution, and naturally airing the colloidal gold solution for later use;
the fifth step, prepare the sample pad, soak the sample pad in 0.01M PBS, pH7.4, 2% Triton X-100, 3% BSA, 0.07% NaN3Taking out the treatment liquid for 3min, and drying at 35-40 deg.C for use;
sixthly, spraying the abietic acid-bovine serum albumin complex on a detection line as a T line, spraying the goat anti-rabbit IgG on a quality control line as a C line, coating for 2 hours at 35-40 ℃, and naturally airing or drying at room temperature to prepare an immune nitrocellulose membrane;
and seventhly, sequentially adhering the sample pad, the colloidal gold combined pad, the immune nitrocellulose membrane and the water absorption pad on the bottom plate, cutting to obtain the reagent strip, and then assembling.
The manufacturing steps of the colloidal gold tool body in the first step are as follows: 1. taking a 300mL triangular flask, adding 100mL double distilled water and 1mL 1% gold chloride solution, filtering with a 0.22 μm filter membrane, and heating to boil; 2. filtering 4mL of 1% sodium citrate with a 0.22-micrometer filter membrane, adding into the solution, rapidly mixing uniformly, heating and keeping boiling for 10-15min, continuously stirring to make the color of the solution in the triangular flask change from light yellow, purple and wine red, taking down the triangular flask, cooling to room temperature, supplementing to the original volume with double distilled water, preparing into wine red colloidal gold solution, and filtering with a 0.22-micrometer filter membrane; 3. and (3) preserving the colloidal gold solution at 4 ℃ by using a reagent bottle for later use, and meanwhile, when floating objects exist on the surface of the colloidal gold solution, the colloidal gold solution is unstable and has poor uniformity and needs to be discarded for re-manufacture.
The material diluted by the colloidal gold solution in the fourth step is as follows: 7% of 1.0mTris liquid, 0.3% of polyethylene glycol 20000, 0.18% of BAS and the balance of water.
In the sixth step, the concentrations of the abietic acid-bovine serum albumin complex and the goat anti-rabbit IgG are respectively 2.1mg/mL and 1.2 mg/mL.
In the sixth step, the abietic acid-bovine serum albumin compound is prepared by the following steps:
mixing abietic acid and carboxymethyl hydroxylamine hydrochloride, and stirring for reaction to obtain a reactant;
and mixing the reactant and bovine serum albumin, and stirring for reaction to obtain the abietic acid-bovine serum albumin compound.
Specifically, taking 6g of a sample to be detected, shearing into pieces, adding 50ml of ethanol, carrying out ultrasonic treatment for 45min, naturally cooling, filtering by using a 0.22-micron microporous filter membrane, evaporating filtrate to dryness to obtain residues, and adding 8ml of ethanol into the residues for dissolving to obtain a sample solution for later use; placing the detection card on a stable working table, arranging a letter T at the position corresponding to the detection line 6 on the surface of the detection card, arranging a letter C at the position corresponding to the quality control line 7, absorbing the sample solution by using a sample adding suction pipe, adding 100 microliters of the sample solution into a sample adding hole of the detection card, standing for 5-10 minutes, and reading a result, wherein a detection reagent strip in the reagent card is a key for realizing the detection of the abietic acid, and the detection line 6 of the reagent strip is coated with the abietic acid-bovine serum albumin complex; fixing colloidal gold-labeled anti-abietic acid monoclonal antibody on an immune colloidal gold combined pad 3 of the reagent strip, adding a sample extracting solution from a sample adding hole to permeate to a sample pad 2 of the reagent strip, firstly fixing the colloidal gold-labeled anti-abietic acid monoclonal antibody on the immune colloidal gold combined pad 3 of a sample to be detected, continuously carrying out chromatography to an immune nitrocellulose membrane 4 through capillary action, sequentially spraying a detection line of abietic acid-bovine serum albumin complex and a quality control line of goat anti-rabbit IgG on the immune nitrocellulose membrane 4, if the sample extracting solution contains abietic acid, enabling the abietic acid to be combined with limited antigen binding sites on the colloidal gold-labeled anti-abietic acid monoclonal antibody, and occupying all the antigen binding sites on the anti-abietic acid monoclonal antibody when the concentration of the abietic acid reaches a threshold concentration designed by a product, therefore, the combination of the colloidal gold-labeled anti-abietic acid monoclonal antibody and the abietic acid-bovine serum albumin compound of the detection line 6 is prevented, the detection line cannot capture colloidal gold particles without red color bands, and the detection result is positive; and if no abietic acid exists in the sample extracting solution or the concentration of the abietic acid is lower than the threshold concentration, the anti-abietic acid monoclonal antibody colloidal gold runs to a detection line along with the sample extracting solution, the detection line captures colloidal gold particles to present a red color band, and the detection result is negative.
And (3) judging a detection result:
if the detection line 6 and the quality control line 7 are both colored, the detection result is judged to be negative, which indicates that the sample does not contain abietic acid or the content of the abietic acid is lower than the detection limit;
if the detection line 6 does not develop color and the quality control line 7 develops color, the detection result is judged to be positive, which indicates that the hormone content of the water product in the sample is equal to or higher than the detection limit;
if the quality control line 6 is not colored, the detection result is invalid, which indicates that the operation is incorrect or the detection card is expired and failed, and the detection is required to be carried out again.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The utility model provides a rosin acid colloidal gold detects card, includes bottom plate (1), sample pad (2), immune colloidal gold and combines pad (3), immune nitrocellulose membrane (4) and absorbs water pad (5), its characterized in that, sample pad (2), immune colloidal gold combine pad (3), immune nitrocellulose membrane (4) and absorb water pad (5) head and the tail in proper order and be fixed in the surface of bottom plate (1), immune colloidal gold combines anti rosin acid monoclonal antibody that pad (3) contains the colloidal gold mark, be wrapped up in rosin acid-bovine serum albumin complex's detection line (6) and sheep anti rabbit IgG's quality control line (7) on immune nitrocellulose membrane (4).
2. The rosin acid colloidal gold test card according to claim 1, wherein the colloidal gold in the immune colloidal gold conjugate pad (3) is in powder form, and the particles are spherical with a diameter of 30 nm.
3. The rosin acid colloidal gold test card according to claim 1, wherein the bottom plate (1) is strip-shaped and made of PVC material, and the sample pad (2) is made of glass fiber material.
4. The preparation method of the abietic acid colloidal gold detection card is characterized by comprising the following steps:
step one, preparing colloidal gold: preparing a colloidal gold solution, adjusting the pH value to 8-9, and then keeping for later use;
second, antibody preparation: preparing an anti-abietic acid monoclonal antibody by adopting an ascites induction method in an animal body, and purifying the obtained antibody by adopting an affinity purification method;
thirdly, adding the obtained anti-abietic acid monoclonal antibody into the colloidal gold solution obtained in the first step, stirring and mixing for 30min at the room temperature of 25-30 ℃, adding polyethylene glycol, stirring and mixing for 30min again, discarding supernatant, and precipitating to obtain a marked anti-abietic acid monoclonal antibody;
fourthly, preparing an immune colloidal gold bonding pad, using glass fiber paper as the colloidal gold bonding pad, soaking the glass fiber paper in a treatment solution with pH of 7.8 and 0.1% TritonX-100 for 3min, taking out the glass fiber paper, drying the glass fiber paper at the temperature of 35-40 ℃, diluting the colloidal gold solution prepared in the first step, putting the diluted colloidal gold solution into the glass fiber paper, soaking the colloidal gold solution for 5min, taking out the colloidal gold solution, and naturally airing the colloidal gold solution for later use;
the fifth stepThe sample pad was prepared by soaking the sample pad in 0.01M PBS, pH7.4, 2% Triton X-100, 3% BSA, 0.07% NaN3Taking out the treatment liquid for 3min, and drying at 35-40 deg.C for use;
sixthly, spraying the abietic acid-bovine serum albumin complex on a detection line as a T line, spraying the goat anti-rabbit IgG on a quality control line as a C line, coating for 2 hours at 35-40 ℃, and naturally airing or drying at room temperature to prepare an immune nitrocellulose membrane;
and seventhly, sequentially adhering the sample pad, the colloidal gold combined pad, the immune nitrocellulose membrane and the water absorption pad on the bottom plate, cutting to obtain the reagent strip, and then assembling.
5. The method for manufacturing the rosin acid colloidal gold test card according to claim 4, wherein the first step of manufacturing the colloidal gold fixture comprises the following steps: 1. taking a 300mL triangular flask, adding 100mL double distilled water and 1mL 1% gold chloride solution, filtering with a 0.22 μm filter membrane, and heating to boil; 2. filtering 4mL of 1% sodium citrate with a 0.22-micrometer filter membrane, adding into the solution, rapidly mixing uniformly, heating and keeping boiling for 10-15min, continuously stirring to make the color of the solution in the triangular flask change from light yellow, purple and wine red, taking down the triangular flask, cooling to room temperature, supplementing to the original volume with double distilled water, preparing into wine red colloidal gold solution, and filtering with a 0.22-micrometer filter membrane; 3. and (3) preserving the colloidal gold solution at 4 ℃ by using a reagent bottle for later use, and meanwhile, when floating objects exist on the surface of the colloidal gold solution, the colloidal gold solution is unstable and has poor uniformity and needs to be discarded for re-manufacture.
6. The method for manufacturing the rosin acid colloidal gold test card according to claim 4, wherein the colloidal gold solution diluting material in the fourth step is: 7% of 1.0mTris liquid, 0.3% of polyethylene glycol 20000, 0.18% of BAS and the balance of water.
7. The method for preparing the rosin acid colloidal gold test card according to claim 4, wherein the concentrations of the rosin acid-bovine serum albumin complex and the goat anti-rabbit IgG in the sixth step are 2.1mg/mL and 1.2mg/mL, respectively.
8. The method for preparing the rosin acid colloidal gold test card according to claim 4, wherein the rosin acid-bovine serum albumin complex in the sixth step is prepared by the following steps:
mixing abietic acid and carboxymethyl hydroxylamine hydrochloride, and stirring for reaction to obtain a reactant;
and mixing the reactant and bovine serum albumin, and stirring for reaction to obtain the abietic acid-bovine serum albumin compound.
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Cited By (1)
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| CN115993447A (en) * | 2023-03-22 | 2023-04-21 | 苏州市药品检验检测研究中心（苏州市药品不良反应监测中心） | Colloidal gold rapid detection kit for abietic acid |
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|---|---|---|---|---|
| CN115993447A (en) * | 2023-03-22 | 2023-04-21 | 苏州市药品检验检测研究中心（苏州市药品不良反应监测中心） | Colloidal gold rapid detection kit for abietic acid |
| CN115993447B (en) * | 2023-03-22 | 2023-05-16 | 苏州市药品检验检测研究中心（苏州市药品不良反应监测中心） | Colloidal gold rapid detection kit for abietic acid |
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