CN112573670A - Method for promoting algae-bacteria symbiotic system to treat pollutants in water by using static magnetic field - Google Patents
Method for promoting algae-bacteria symbiotic system to treat pollutants in water by using static magnetic field Download PDFInfo
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- C02F1/481—Treatment of water, waste water, or sewage with magnetic or electric fields using permanent magnets
- C02F1/482—Treatment of water, waste water, or sewage with magnetic or electric fields using permanent magnets located on the outer wall of the treatment device, i.e. not in contact with the liquid to be treated, e.g. detachable
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Abstract
The invention belongs to the technical field of water pollution environment treatment, and particularly relates to a method for removing water pollutants by using a magnetic field reinforced algae-bacteria symbiotic system, which mainly comprises the steps of constructing the algae-bacteria symbiotic system, wherein the adding ratio (density) of chlorella to bacillus licheniformis is 3: the chlorella is placed in a polluted water body, magnetic treatment is carried out for 2 hours every day under the condition of a static magnetic field, the growth of chlorella and bacillus licheniformis is promoted by utilizing magnetism, meanwhile, extracellular polymer secretion of chlorella is promoted, the stability of a symbiotic system of the chlorella is consolidated, and the efficiency of treating pollutants in water is improved. The method has the advantages that the static magnetic field is added for treatment while the traditional algae bacteria symbiotic system is used for treating pollutants, energy is not consumed, the pollutant treatment efficiency is effectively improved, the total organic carbon concentration of the water body is lower compared with the traditional algae bacteria symbiotic process, the method is simple, the operation is flexible, the environment is friendly, and the operation cost is low.
Description
Technical Field
The invention relates to the field of water environment technical research, in particular to a method for promoting a phycomycete symbiotic system to treat pollutants in water by using a static magnetic field.
Background
Many water bodies in China face the threat of water body eutrophication. In rivers and lakes, many have the problem of nitrogen and phosphorus pollution. Researches show that the concentration of nitrogen pollutants in Qiantangjiang tends to rise year by year due to excessive use of chemical fertilizers and discharge of industrial wastewater. As the pollution of a plurality of river rivers entering the sea is serious, the water body of the Bohai Bay is eutrophicated when the water body reaches the eutrophication level. In 2007, the water use crisis caused by the blue algae outbreak in the lake Taihu river basin also rings people.
The sewage treatment by utilizing the algae-bacteria symbiotic system is a novel process which is concerned more. The algae absorbs nutrients such as nitrogen and phosphorus in the water and releases oxygen for the growth of fungi, and on the other hand, the bacteria can degrade some organic matters which are difficult to degrade in the water, so that the effect of removing COD is very obvious. On one hand, the algae bacteria can be recycled, waste is changed into valuable, and the algae bacteria become a sharp tool for removing pollutants in water. On the other hand, the treatment cost is low, and the green color can be recycled. However, the traditional sewage treatment method of the phycobiont system has a long treatment period and has many factors influencing the sewage treatment efficiency of the phycobiont system. For example, researchers have found that photoperiod affects the efficiency of nutrient removal and biomass in phycomycete systems. However, the magnetic field can be continuously used without the need of external energy, and is beneficial to the sewage treatment process of the phycomycete system by few people. The invention accelerates the growth of the phycomycetes by utilizing the external magnetic field, thereby improving the degradation efficiency of pollutants and shortening the period.
Disclosure of Invention
In order to improve the removal efficiency of pollutants such as nitrogen and phosphorus in water and shorten the period of sewage treatment of an algae bacterium symbiotic system, the invention aims to disclose a method for promoting the algae bacterium symbiotic system to treat the pollutants in the water by using a static magnetic field. The permanent magnetic field does not need external energy, can be repeatedly used, and can accelerate the symbiotic growth of algae and bacteria, thereby achieving the purpose of removing pollutants in water. The method is simple, flexible to operate and low in operation cost.
The invention provides a method for removing water pollutants by using a magnetic field reinforced phycobiont system, which comprises the following specific steps:
(1) inoculating chlorella, and culturing to stationary phase to obtain cultured chlorella; simultaneously inoculating bacillus licheniformis, and culturing the bacillus licheniformis to a stationary phase to obtain the cultured bacillus licheniformis;
(2) putting the chlorella and the bacillus licheniformis cultured in the step (1) into an experimental container, adding sewage at the same time, and uniformly stirring;
(3) and (3) placing the experimental container in the step (2) on a steel frame, placing the experimental container in a magnetic field, namely arranging a fixed magnetic pole at the bottom of the experimental container, arranging a movable magnetic pole at the upper part of the experimental container, fixing the movable magnetic pole at the bottom of a spiral adjusting rod, adjusting the movable magnetic pole through the spiral adjusting rod, and continuously analyzing the water quality after 4 days of treatment.
In the invention, the culture conditions of the chlorella in the step (1) are as follows: inoculated in BG11 medium at a brightness ratio of 12: 12, light intensity of 120. mu. mol photons m−2 s−1And culturing at 26-28 deg.c for 10 days.
In the invention, the culture conditions of the bacillus licheniformis in the step (1) are as follows: inoculating into LB culture medium, and culturing in gas bath constant temperature shaking box at 28 deg.C and 120 rpm for 24 hr.
In the invention, in the step (2), the addition ratio (density) of the chlorella to the bacillus licheniformis is 3: 1, controlling the initial density of chlorella and bacillus licheniformis in sewage to be 3 multiplied by 10 respectively5cells/mL and 105 cells/mL。
In the invention, the magnetic field intensity in the step (3) is controlled to be 400 Gs, 800 Gs and 1500 Gs respectively, the treatment is carried out for two hours every day for 4 days continuously, and the day 0 of inoculation is calculated on the day.
In the invention, the water quality is detected on the 4 th day, and the water quality is contrastively analyzed, and the content of extracellular polysaccharide is measured to evaluate the stability of the phycobiont system.
The invention has the beneficial effects that: the invention promotes the growth of algae through the static magnetic field, so that the algae symbiotic system is more stable, thereby accelerating the removal efficiency of pollutants in water. The permanent magnetic field does not need external energy, can be repeatedly used, and has the advantages of simple method, flexible operation and low operation cost. The method has great improvement on the sewage treatment process of the phycobiont system, thereby promoting the sewage treatment of small and medium-sized projects in China.
Drawings
In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below.
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic diagram of an embodiment of the present invention.
Reference numbers in the figures: 1 is the screw adjusting pole, 2 is portable magnetic pole (N), 3 is the steelframe, 4 are phycomycete intergrowth system, 5 are the experimental container, 6 are fixed magnetic pole (S), 7 are the N magnetic pole, 8 are the baffle, 9 are the rivers direction, 10 are phycomycete intergrowth system, 11 are the S magnetic pole.
Detailed Description
The invention is further illustrated by the following examples.
Example 1: the method for promoting the algae and bacteria symbiosis system to treat pollutants in water by using the static magnetic field is disclosed by the embodiment of the invention.
(1) Culturing chlorella vulgaris: the chlorella is inoculated in BG11 culture medium, and the ratio of light to dark is 12: 12, light intensity of 120. mu. mol photons m−2 s−1Culturing at 26-28 deg.C for 10 days;
(2) inoculating bacillus licheniformis into an LB culture medium, and culturing for 24 hours in a gas bath constant-temperature shaking box with the temperature of 28 ℃ and the rotation speed of 120 rpm. Composition of LB medium per liter: 10 g tryptone, 5 g yeast extract, 10 g sodium chloride;
(3) collecting the polluted water body, and mixing the algae obtained in the step (1) with the stepAnd (3) the bacillus licheniformis obtained in the step (2) has the ratio of phycomycetes (density) of 3: 1, putting the mixture into sewage, and uniformly stirring the mixture until the initial densities of the algae and the bacillus licheniformis in the sewage are respectively 3 multiplied by 105cells/mL and 105 cells/mL；
(4) The method comprises the following steps of placing algae bacterium sewage placed in an experimental container in a static magnetic field shown in a figure 1, wherein the device consists of a spiral adjusting rod 1, a movable magnetic pole (N) 2, a steel frame 3, an experimental container 4 and a fixed magnetic pole (S) 5, the experimental container 4 is placed on the steel frame 3, the experimental container 4 is placed in a magnetic field, namely the fixed magnetic pole 5 is arranged at the bottom of the experimental container 4, the movable magnetic pole 2 is arranged at the upper part of the experimental container 4, the movable magnetic pole 2 is fixed at the bottom of the spiral adjusting rod 1, the movable magnetic pole 2 is adjusted through the spiral adjusting rod 1, the magnetic field strength of the movable magnetic pole 2 is respectively 400 Gs, 800 Gs and 1500 Gs, the algae bacterium sewage is;
(5) the change of indexes such as water quality, algae density and the like is measured in the middle.
The results are as follows:
(1) total phosphorus: the initial total phosphorus concentration of the sewage is 0.865 +/-0.003 mg/L, and the results of the magnetic treatment for four days, the magnetic treatment without the magnetic treatment, the magnetic field intensity of 400 Gs, the magnetic field intensity of 800 Gs and the magnetic field intensity of 1500 Gs are 0.268 +/-0.007, 0.191 +/-0.042, 0.182 +/-0.025 and 0.154 +/-0.044 mg/L respectively. The total phosphorus removal rates were 69.02%, 77.97%, 78.91%, and 82.21%, respectively. The total phosphorus removal rate after 1500 Gs magnetic field treatment is 19.10 percent higher than that without the magnetic field treatment.
(2) Total nitrogen-initial total phosphorus concentration of the wastewater was 34.642 + -0.472 mg/L. The results of the four-day magnetic treatment are 33.708 +/-0.472, 33.248 +/-0.484, 33.388 +/-0.370 and 33.332 +/-1.160 mg/L respectively without magnetic treatment and after the magnetic field intensity of 400 Gs, the magnetic field intensity of 800 Gs and the magnetic field intensity of 1500 Gs. The removal effect is not significant.
(3) Total organic carbon: the initial total organic carbon concentration of the sewage is 6.085 +/-0.192 mg/L. The results of the magnetic treatment of four days, no magnetic treatment, the treatment of magnetic field strength of 400 Gs, magnetic field strength of 800 Gs and magnetic field strength of 1500 Gs are 11.918 +/-0.913, 9.600 +/-1.253, 10.662 +/-1.901 and 10.563 +/-0.548 mg/L respectively. The total organic carbon concentration is increased, which is caused by the decay of algae. And the total organic carbon after being treated by the magnetic fields of 400 Gs, 800 Gs and 1500 Gs is respectively 19.45 percent, 10.54 percent and 11.36 percent lower than that without being treated.
(4) The algae density is as follows: the original algae density is 3 × 105 cells/L, and the results of the magnetic treatment for four days, the magnetic treatment without the magnetic treatment, the magnetic field intensity of 400 Gs, the magnetic field intensity of 800 Gs and the magnetic field intensity of 1500 Gs are (31.6 + -1.5) × 105、（39.0±2.8）×105、（45.6±4.5）×105、（49.3±1.2）×105mg/L. The densities of the algae treated by the magnetic fields of 400 Gs, 800 Gs and 1500 Gs are respectively 23.31 percent, 44.20 percent and 56.01 percent higher than those of the algae which is not treated.
Claims (6)
1. A method for removing water pollutants by using a magnetic field reinforced algae-bacteria symbiotic system is characterized by comprising the following specific steps:
(1) inoculating chlorella, and culturing to stationary phase to obtain cultured chlorella; simultaneously inoculating bacillus licheniformis, and culturing the bacillus licheniformis to a stationary phase to obtain the cultured bacillus licheniformis;
(2) putting the chlorella and the bacillus licheniformis cultured in the step (1) into an experimental container, adding sewage at the same time, and uniformly stirring;
(3) and (3) placing the experimental container in the step (2) on a steel frame, placing the experimental container in a magnetic field, namely arranging a fixed magnetic pole at the bottom of the experimental container, arranging a movable magnetic pole at the upper part of the experimental container, fixing the movable magnetic pole at the bottom of a spiral adjusting rod, adjusting the movable magnetic pole through the spiral adjusting rod, and continuously analyzing the water quality after 4 days of treatment.
2. The method according to claim 1, wherein the chlorella culture conditions in step (1) are: inoculated in BG11 medium at a brightness ratio of 12: 12, light intensity of 120. mu. mol photons m−2 s−1And culturing at 26-28 deg.c for 10 days.
3. The method according to claim 1, wherein the Bacillus licheniformis culture conditions in step (1) are: inoculating into LB culture medium, and culturing in gas bath constant temperature shaking box at 28 deg.C and 120 rpm for 24 hr.
4. The method according to claim 1, wherein the ratio of the chlorella to the bacillus licheniformis in step (2) is 3: 1, controlling the initial density of chlorella and bacillus licheniformis in sewage to be 3 multiplied by 10 respectively5cells/mL and 105 cells/mL。
5. The method according to claim 1, wherein the magnetic field strength in step (3) is controlled to be 400 Gs, 800 Gs and 1500 Gs respectively, the treatment is carried out for two hours per day and is continuously carried out for 4 days, and the inoculation is carried out on day 0.
6. The method of claim 4, wherein the water quality is measured on day 4, analyzed by contrast, and the exopolysaccharide content is measured to evaluate the stability of the phycobiont system.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113932A (en) * | 2013-02-01 | 2013-05-22 | 哈尔滨工业大学深圳研究生院 | Method and system for producing biodiesel by cultivating microalgae by using urban sewage |
| US20150275166A1 (en) * | 2014-03-25 | 2015-10-01 | Boise State University | Ultraviolet radiation pre-treatment of wastewater, improving its utility for algal cultivation |
| CN107129950A (en) * | 2017-06-09 | 2017-09-05 | 复旦大学 | A kind of active phycomycete community and preparation method for purifying domestic sewage |
| CN110257251A (en) * | 2019-07-11 | 2019-09-20 | 哈尔滨工业大学（深圳） | A method of oxygen performance is produced using algae in magnetic field United microwave enhancing municipal sewage |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113932A (en) * | 2013-02-01 | 2013-05-22 | 哈尔滨工业大学深圳研究生院 | Method and system for producing biodiesel by cultivating microalgae by using urban sewage |
| US20150275166A1 (en) * | 2014-03-25 | 2015-10-01 | Boise State University | Ultraviolet radiation pre-treatment of wastewater, improving its utility for algal cultivation |
| CN107129950A (en) * | 2017-06-09 | 2017-09-05 | 复旦大学 | A kind of active phycomycete community and preparation method for purifying domestic sewage |
| CN110257251A (en) * | 2019-07-11 | 2019-09-20 | 哈尔滨工业大学（深圳） | A method of oxygen performance is produced using algae in magnetic field United microwave enhancing municipal sewage |
Non-Patent Citations (2)
| Title |
|---|
| 刘燕: "小球藻与地衣芽孢杆菌联合体系脱氮除磷效果的研究", 《中国优秀硕士学位论文全文数据库（工程科技Ⅰ辑）》 * |
| 陈仰光: "城市污水培养小球藻和斜生栅藻的磁处理效应研究", 《中国优秀硕士学位论文全文数据库（工程科技Ⅰ辑）》 * |
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