CN112569266A - Uighur medicine extract, preparation method thereof and application thereof in preparing anti-inflammatory analgesic medicines - Google Patents
Uighur medicine extract, preparation method thereof and application thereof in preparing anti-inflammatory analgesic medicines Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
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- Epidemiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of traditional Chinese medicine pharmacy, relates to a radix Abelmoschi Manihot seed extract, and particularly relates to a radix Abelmoschi Manihot seed extract as well as a preparation method and medicinal application thereof. According to the invention, macroporous resin is used for extracting related active parts of the hollyhock seed water extract by using ethanol with different concentrations, and active parts with remarkable anti-inflammatory and analgesic effects, namely elution parts of 25% ethanol, are screened from different extraction parts through in vivo experiments of mice. According to the invention, the anti-inflammation and analgesic effect experiment is carried out on a mouse pathological model through extracts of different parts of a radix althaeae roseae to obtain an active part with an obvious anti-inflammation and analgesic effect; furthermore, the active part can be used for preparing anti-inflammatory and analgesic drugs.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine pharmacy, relates to a Uighur medicine extract, a preparation method thereof and application thereof in preparing anti-inflammatory and analgesic medicines, and particularly relates to an extraction method of a 25% ethanol extraction part of hollyhock seeds and application of the extract in preparing anti-inflammatory and analgesic medicines.
Background
Inflammation (inflammation), which is commonly referred to as "inflammation," is a defense response of the body to stimuli, manifested by redness, swelling, heat, pain, and dysfunction. The inflammation may or may not be infectious inflammation caused by infection. Inflammation is generally beneficial and an automatic defense response of the human body, but sometimes, inflammation is also harmful, such as attack on the body's own tissues, inflammation occurring in transparent tissues, and the like. The inflammation of the body is treated by anti-inflammation, and if hormone treatment is adopted, the problems of toxic and side effects or reduced hormone sensitivity can be caused, and the traditional medicine treatment is the current trend.
Hollyhock (academic name: Althaea rosea (Linn.) Cavan.) is a biennial upright plant of hollyhock of Malvaceae (Malvaceae), and is named as cloaca flower, jatropha, chessflower, chingma flower, juniper flower, lipsticks, fructus evodiae, and weizu, etc. The hollyhock has ornamental value and high medicinal value; the hollyhock is used as a medicine by using roots, stems, leaves, flowers and seeds. The hollyhock seed is a dry seed of the plant, is a traditional Uygur medicinal material, has multiple functions in Uygur medicine clinical and folk, has the functions of clearing heat, detoxifying, inducing diuresis, promoting urination, smoothing intestinal tracts, inducing diuresis, removing urinary calculus, promoting lactation and the like, is used for dysuria, eliminating facial freckles, and is used for treating diseases such as edema, stranguria, leukorrhagia, galactostasis, scabies, innominate toxic swelling and the like.
The inventor separates the active part with obvious anti-inflammatory and analgesic effects from the hollyhock seeds through experimental research, and can further research and develop a patent medicine or a compound preparation.
Disclosure of Invention
The invention aims to provide a Uighur medicine extract, a preparation method thereof and application thereof in preparing anti-inflammatory and analgesic medicines, and particularly relates to an extraction method of a 25% ethanol extraction part of hollyhock seeds and application of the extract in preparing anti-inflammatory and analgesic medicines.
The Viburnum orientale extract is a water extract of hollyhock seeds, and particularly is an elution part of hollyhock seeds with 25% ethanol.
The preparation method of the Uighur medicine extract is characterized by at least comprising the following steps:
(1) taking crude drugs; the crude drug is dry seeds of Althaea officinalis;
(2) extracting Althaea officinalis seed with distilled water, and extracting the residue with ethanol; filtering and mixing the two extracting solutions to obtain a hollyhock seed total extract; concentrating the total extract to obtain a sample solution;
(3) collecting the supernatant, passing through D-101 macroporous adsorbent resin, sequentially eluting with water and 25% ethanol to obtain 25% ethanol extract;
(4) filtering the 25% ethanol eluate of Althaea officinalis, concentrating, and drying under reduced pressure to obtain 25% ethanol eluate.
In the step (2) of the preparation method, the total extract is prepared by adding 12 times of water, decocting for 2 times, each time for 1.5 hours, and filtering. And finally, adding 95% ethanol in an amount which is 8 times that of the residue, decocting for 0.5 hour, filtering, combining the filtrates, concentrating, and drying under reduced pressure to prepare the total extract.
In step (2) of the preparation method of the present invention, the crude drug concentration of the sample solution is 0.8 Ag/ml.
In the step (3) of the preparation method, the adsorption flow rate of the macroporous resin is 2 column volumes per hour.
In the step (3) of the preparation method of the present invention, the dosages of water and 25% ethanol for elution by the macroporous resin are each 4 column volumes, respectively.
In the step (4) of the preparation method, distilled water is firstly used, and then 25% ethanol is used for elution.
In the step (4) of the preparation method of the invention, the flow rate of elution of the macroporous resin is 2 column volumes per hour.
According to the invention, the edema resisting and pain relieving effects of different parts of hollyhock seeds are observed through a mouse ear xylene-coated edema model and a mouse abdominal cavity acetic acid-injected pain model, and experimental results show that the effect of the 25% ethanol elution part is obviously higher than that of other parts; and then injecting acetic acid into the abdominal cavity of the mouse to cause a capillary permeability model to observe the protection of the capillary permeability of the 25% ethanol part, sensitizing the egg protein of the mouse to establish an asthma model to observe the protection of the lung cells and organs of the mouse, wherein the 25% ethanol part has obvious protection.
The results of the anti-inflammatory test are shown in Table 1
TABLE 1
Group of | n | Dosage to be administered | Swelling degree/mg | Inhibition rate/%) |
Control group | 10 | — | 2.3±1.09 | — |
Indometacin | 8 | 10mg /Kg | 1.3±0.39* | 45.1%* |
Althea officinalis total extract | 10 | 20 ɡ/Kg | 1.6±1.12 | 29.4% |
Water eluate of Althaea officinalis | 9 | 20 ɡ/Kg | 1.2±0.45* | 45.5%* |
Althea seed 25% ethanol eluate | 8 | 20 ɡ/Kg | 0.9±0.43** | 62.1%** |
Althea officinalis 50% ethanol eluate | 8 | 20 ɡ/Kg | 1.2±0.32* | 44.6%* |
75% ethanol eluate of Althea officinalis | 10 | 20 ɡ/Kg | 1.2±0.79* | 49.4%* |
Althea officinalis 95% ethanol eluate | 8 | 20 ɡ/Kg | 1.6±0.86 | 30.9% |
P <0.001 compared to model group.
According to the invention, macroporous resin is used for extracting related active parts of the hollyhock seed water extract by using ethanol with different concentrations, and active parts with remarkable anti-inflammatory and analgesic effects, namely elution parts of 25% ethanol, are screened from different extraction parts through in vivo experiments of mice. According to the invention, the anti-inflammation and analgesic effect experiment is carried out on a mouse pathological model through extracts of different parts of a radix althaeae roseae to obtain an active part with an obvious anti-inflammation and analgesic effect; furthermore, the active part can be used for preparing anti-inflammatory and analgesic drugs.
Detailed Description
Example 1: preparing each part of hollyhock
(1) Medicinal materials and reagents: althaea officinalis (supplied by Xinjiang Sael Uygur medicine Co., Ltd., lot number: skz-yp-170622), D101 macroporous adsorbent resin, and 95% ethanol.
(2) The instrument comprises the following steps: an electronic balance of the EL204 type (METTLER TOLEDO); an electric heating constant temperature water bath (Yongguang medical instruments, Inc., Beijing); SZ-93A automatic double pure water distiller (Shanghai Yangrong biochemical instruments factory) and 101 type electrothermal blowing dry box (Yongguanming medical instruments, Inc., Beijing City). DZF-6021 vacuum drying oven; KDM type temperature adjustable electric heating jacket (Shandong Juancheng Hualu electric heating apparatus Co., Ltd.) rotary evaporator (Shanghai Yangrong Biochemical apparatus factory); model 101 electrothermal blowing dry box (Yongguang medical instruments, Inc., Beijing).
(3) Preparing various parts of hollyhock seeds: taking 4Kg of crushed dry hollyhock seed medicinal material, adding 12 times of distilled water to soak overnight, extracting under reflux twice, each time for 1.5 hours, filtering, adding 8 times of 95% ethanol on the residue, decocting for 0.5 hours, filtering, combining the filtrates, and concentrating to obtain 640g of total extract. Regulating crude drug concentration to 0.8 Ag/ml, pouring the concentrated solution into treated macroporous adsorbent resin column, and standing for 3-4 hr. And after the total leaching liquor is fully absorbed by macroporous adsorption resin, sequentially eluting with 4BV of distilled water, 25%, 50%, 75% and 95% ethanol, concentrating and drying the eluent respectively to obtain 380g of water separation part, 22g of 25% ethanol separation part, 20g of 50% ethanol separation part, 5g of 75% ethanol separation part and 4g of 95% ethanol separation part, and sealing and storing each part in a refrigerator for co-drug effect experiments.
Example 2: influence of swelling of auricles of mice at various parts of hollyhock
(1) Experimental animals: a healthy Kunming mouse (provided by experimental animal center of Xinjiang Uygur medicine university) is adopted to perform experiments in animal houses of Xinjiang Uygur medicine special school, the body mass is 20 +/-2 g, the illumination time is fixed, and water and food are freely taken.
(2) Reagent: indomethacin (produced by shanxi shadongtai pharmaceutical co ltd, lot 20160502); xylene (Dudouke Kelong chemical reagent plant, lot number 2016101301;) physiological saline (Shiyaoyanghu pharmaceutical Limited public production, lot number 31317061801).
(3) The instrument comprises the following steps: an electronic balance of the EL204 type (METTLER TOLEDO); KQ5200 model desk type mechanical ultrasonic cleaner (supersonic equipment limited bridge, guan city); an electric heating constant temperature water bath (Yongguang medical instruments, Inc., Beijing).
(4) Preparing a liquid medicine: respectively taking 21.33g of hollyhock seed total extract, 12.66g of water elution part, 733mg of 25% ethanol elution part, 666mg of 50% ethanol elution part, 166mg of 75% ethanol elution part and 133mg of 95% ethanol elution part, respectively dissolving (or suspending) with water to fix the volume in a 100ml volumetric flask, and storing in a refrigerator, and performing ultrasonic treatment for 10 minutes before each gastric lavage. The indometacin is pressed into powder and prepared into 0.8 mg/ml suspension by using physiological saline.
(5) Experimental grouping and dosing: 80 Kunming mice, weighing 22 +/-25 Ag, were bred adaptively for one week and then randomly divided into 8 groups, namely a model group, an indomethacin group, a total extract group, a water eluate group, a 25% ethanol eluate group, a 50% ethanol eluate group, a 75% ethanol eluate group and a 95% ethanol eluate group, and were each subjected to intragastric administration of 0.3 ml/mouse. The model group is administered with physiological saline of the same volume, the positive group is administered with indometacin (10 mg/Kg), and the administration group is administered with extract of each part of radix Althaeae Roseae (equivalent to 20g/Kg of raw medicinal material) for 1 time/day for 7 days. 1h after the last administration, 0.05ml of xylene was applied to the front and back of the left ear of the white mouse to cause inflammation, and the right ear was used as a control. After 1.5h of xylene coating, the white mouse is killed by removing the cervical vertebra, the ears are cut off, round ear pieces are punched at the same positions of the left ear and the right ear by a puncher, weighing is carried out, and the weight difference of the left ear piece and the right ear piece is calculated to judge the swelling degree.
(6) The results of the anti-inflammatory test are shown in Table 1
TABLE 1
Group of | n | Dosage to be administered | Swelling degree/mg | Inhibition rate/%) |
Control group | 10 | — | 2.3±1.09 | — |
Indometacin | 8 | 10mg /Kg | 1.3±0.39* | 45.1%* |
Althea officinalis total extract | 10 | 20 ɡ/Kg | 1.6±1.12 | 29.4% |
Water eluate of Althaea officinalis | 9 | 20 ɡ/Kg | 1.2±0.45* | 45.5%* |
Althea seed 25% ethanol eluate | 8 | 20 ɡ/Kg | 0.9±0.43** | 62.1%** |
Althea officinalis 50% ethanol eluate | 8 | 20 ɡ/Kg | 1.2±0.32* | 44.6%* |
75% ethanol eluate of Althea officinalis | 10 | 20 ɡ/Kg | 1.2±0.79* | 49.4%* |
Althea officinalis 95% ethanol eluate | 8 | 20 ɡ/Kg | 1.6±0.86 | 30.9% |
P <0.001 compared to model group.
Example 3: influence of various parts of hollyhock on mouse writhing response
(1) Experimental animals: a healthy Kunming mouse (provided by experimental animal center of Xinjiang Uygur medicine university) is adopted to perform experiments in animal houses of Xinjiang Uygur medicine special school, the body mass is 20 +/-2 g, the illumination time is fixed, and water and food are freely taken.
(2) Reagent: aspirin enteric-coated tablet (manufactured by bayer pharmaceutical health care limited, lot No. 85551191); glacial acetic acid (manufactured by Dacron chemical Co., Ltd., Tianjin, lot number 20180502); normal saline (limited public production of Shiyao silver lake, lot number 31317061801).
(3) The instrument comprises the following steps: an electronic balance of the EL204 type (METTLER TOLEDO); KQ5200 model desk type mechanical ultrasonic cleaner (supersonic equipment limited bridge, guan city); an electric heating constant temperature water bath (Yongguang medical instruments, Inc., Beijing).
(4) Preparing a liquid medicine: respectively taking 21.33g of hollyhock seed total extract, 12.66g of water elution part, 733mg of 25% ethanol elution part, 666mg of 50% ethanol elution part, 166mg of 75% ethanol elution part and 133mg of 95% ethanol elution part, respectively dissolving (or suspending) with water to fix the volume in a 100ml volumetric flask, and storing in a refrigerator, and performing ultrasonic treatment for 10 minutes before each gastric lavage. Aspirin is pressed into powder and then prepared into 4.0 mg/ml suspension by using physiological saline.
(5) Experimental grouping and dosing: 80 Kunming mice, weighing 22 +/-25 Ag, were bred adaptively for one week and then randomly divided into 8 groups, namely a model group, an indomethacin group, a total extract group, a water eluate group, a 25% ethanol eluate group, a 50% ethanol eluate group, a 75% ethanol eluate group and a 95% ethanol eluate group, and were each subjected to intragastric administration of 0.3 ml/mouse. The same volume of saline was administered to the model group, the positive control group was administered with gastric aspirin enteric coated tablet (50 mg/Kg), and the administered group was administered with extract from various parts of Althaea officinalis (equivalent to 20 Ag/Kg), 1 time/day, for 7 consecutive days. 1h after the last administration, 0.1ml/10g of 1.2% glacial acetic acid is injected into the abdominal cavity of each white mouse to cause pain, and the times of writhing within 20min after the glacial acetic acid is injected are recorded (writhing reaction is carried out when the mouse has the symptoms of abdominal indent, trunk and hind limb stretch, and hip rise).
(6) The results of the analgesic test are shown in Table 2
TABLE 2
Group of | n | Dosage form | Number of wriggling within 20min | Inhibition rate |
Blank control group (model group) | 8 | — | 45.0±15.92 | — |
Aspirin group | 8 | 50㎎/Kg | 17.0±8.83** | 62.2%** |
Althea officinalis total extract | 8 | 20ɡ/Kg | 44.8±7.83 | 0.5% |
Water eluate of Althaea officinalis | 8 | 20ɡ/Kg | 37.5±6.83 | 16.6% |
Althea seed 25% ethanol eluate | 8 | 20ɡ/Kg | 22.32±8.57* | 50.4%* |
Althea officinalis 50% ethanol eluate | 8 | 20ɡ/Kg | 29.0±3.58* | 35.6%* |
75% ethanol eluate of Althea officinalis | 8 | 20ɡ/Kg | 39.5±13.11 | 12.2% |
Althea officinalis 95% ethanol eluate | 8 | 20ɡ/Kg | 32.2±5.63 | 28.4% |
And comparing with the model group, wherein p is less than 0.05, and p is less than 0.01.
Example 4: althaea officinalis active site protecting effect on capillary permeability
(1) Experimental animals: a healthy Kunming mouse (provided by experimental animal center of Xinjiang Uygur medicine university) is adopted to perform experiments in animal houses of Xinjiang Uygur medicine special school, the body mass is 20 +/-2 g, the illumination time is fixed, and water and food are freely taken.
(2) Reagent: aspirin enteric-coated tablet (manufactured by bayer pharmaceutical health care limited, lot No. 85551191); normal saline (limited pharmaceutical production of Shiyao Yinhu, batch No. 31317061801), glacial acetic acid (production of Daoyeng chemical reagent factory, Tianjin, batch No. 20180502); evans blue.
(3) The instrument comprises the following steps: an electronic balance of the EL204 type (METTLER TOLEDO); t6 new century uv-vis spectrophotometer (beijing prussian instruments ltd), KQ5200 model desk type mechanical ultrasonic cleaner (tokyo koku qiao ultrasonic equipment ltd); an electric heating constant temperature water bath (Yongguang medical instruments, Inc., Beijing).
(4) Preparing a liquid medicine: respectively taking 21.33g of hollyhock seed total extract, 12.66g of water elution part, 733mg of 25% ethanol elution part, 666mg of 50% ethanol elution part, 166mg of 75% ethanol elution part and 133mg of 95% ethanol elution part, respectively dissolving (or suspending) with water to fix the volume in a 100ml volumetric flask, and storing in a refrigerator, and performing ultrasonic treatment for 10 minutes before each gastric lavage. Aspirin is pressed into powder and then prepared into 4.0 mg/ml suspension by using physiological saline.
(5) Experimental grouping and dosing: 90 Kunming mice, weighing 22 +/-25 Ag, were bred adaptively for one week and then randomly divided into 9 groups, namely a blank group, a model group, an indomethacin group, a total extract group, a water eluate group, a 25% ethanol eluate group, a 50% ethanol eluate group, a 75% ethanol eluate group and a 95% ethanol eluate group, and the administration of each group was performed by intragastric administration of 0.3 ml. The same volume of saline was administered to the model group and the blank group, the positive control group was administered with gastric aspirin enteric coated tablets (50 mg/Kg), and the administered group was administered with extracts from various parts of the hollyhock seed (equivalent to bulk Ag 20/Kg) 1 time/day for 7 consecutive days. The last administration is for 1h, 0.1ml/10g of 0.5% Evans is injected into the tail vein of the mouse, 0.1ml/10g of 0.6% glacial acetic acid is injected into the abdominal cavity of 8 groups except the blank group, the mouse is killed after cervical vertebra is removed in 20min, the abdominal cavity is cut off, the abdominal cavity is flushed by 6ml of normal saline for several times, the washing solution is collected and the volume is 6ml, and the mouse is centrifuged at 2500r/min for 10 min. Measuring absorbance at 590nm with ultraviolet spectrophotometer, and determining the influence of the drug on capillary permeability according to the absorbance, as shown in Table 3;
TABLE 3
Group of | n | Dose (Ag/Kg) | Absorbance of the solution |
Blank control group | 10 | — | 0.82±0.53 |
Model set | 8 | — | 1.67±0.57 |
Aspirin group | 8 | 50㎎/Kg | 0.98±0.47** |
Althea officinalis total extract | 8 | 20ɡ/Kg | 1.61±0.75 |
Water eluate of Althaea officinalis | 8 | 20ɡ/Kg | 1.58±0.92 |
Althea seed 25% ethanol eluate | 8 | 20ɡ/Kg | 1.09±0.54* |
Althea officinalis 50% ethanol isolate | 8 | 20ɡ/Kg | 1.04±0.51* |
Althea seed 75% ethanol isolate | 8 | 20ɡ/Kg | 1.07±0.39* |
Althaea officinalis 95% ethanol isolate | 8 | 20ɡ/Kg | 1.28±0.71 |
And comparing with the model group, wherein p is less than 0.05, and p is less than 0.01.
Claims (10)
1. A Uighur medicine extract is characterized by being a water extract of hollyhock seeds.
2. The Uighur medicine extract as claimed in claim 1, wherein the Uighur medicine extract is an elution part of 25% ethanol of hollyhock seed.
3. The preparation method of the Uighur medicine extract is characterized by comprising the following steps:
(1) taking crude drugs; the crude drug is dry seeds of Althaea officinalis;
(2) extracting Althaea officinalis seed with distilled water, and extracting the residue with ethanol; filtering and mixing the two extracting solutions to obtain a hollyhock seed total extract; concentrating the total extract to obtain a sample solution;
(3) collecting the supernatant, passing through D-101 macroporous adsorbent resin, sequentially eluting with water and 25% ethanol to obtain 25% ethanol extract;
(4) filtering the 25% ethanol eluate of Althaea officinalis, concentrating, and drying under reduced pressure to obtain 25% ethanol eluate.
4. The preparation method according to claim 3, wherein in the step (2), the total extract is prepared by adding 12 times of water, decocting for 2 times, each time for 1.5 hours, and filtering; and finally, adding 95% ethanol in an amount which is 8 times that of the residue, decocting for 0.5 hour, filtering, combining the filtrates, concentrating, and drying under reduced pressure to prepare the total extract.
5. The preparation method according to claim 3, wherein in step (2), the crude drug concentration of the sample solution is 0.8 Ag/ml.
6. The production method according to claim 3, wherein in the step (3), the macroporous resin adsorption flow rate is 2 column volumes/hour.
7. The method according to claim 3, wherein the dosages of water and 25% ethanol for eluting the macroporous resin in the step (3) are each 4 column volumes.
8. The method according to claim 3, wherein the elution in the step (4) is performed with distilled water and then with 25% ethanol.
9. The method according to claim 3, wherein in the step (4), the flow rate of elution from the macroporous resin is 2 column volumes/hour.
10. The use of the Uighur medicine extract of claim 1 in the preparation of anti-inflammatory analgesic drugs.
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Citations (2)
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CN101757058A (en) * | 2009-11-13 | 2010-06-30 | 段洪东 | General flavone extract of hollyhock flower, preparation thereof and application |
CN105287678A (en) * | 2015-12-03 | 2016-02-03 | 济南星懿医药技术有限公司 | Medicinal composition for treating senile constipation |
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