CN112568228A

CN112568228A - Insecticidal composition

Info

Publication number: CN112568228A
Application number: CN201910932239.5A
Authority: CN
Inventors: 罗昌炎; 詹姆斯.T.布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2019-09-29
Filing date: 2019-09-29
Publication date: 2021-03-30
Anticipated expiration: 2039-09-29
Also published as: CN116473066A; CN112568228B; CN116508767A; CN116473065A

Abstract

The invention relates to an insecticidal composition, which contains active ingredients of a compound shown in formula I and a compound II, wherein the compound II is selected from trifluoro-benzene pyrimidine, thiocyclam, hydrazine hydrate inhibitor and chlorfenapyrAt least one of amides. The invention also relates to application of the insecticidal composition in preventing or controlling pests of the families of snout moth, plutella, noctuidae, diabrotidae, plant hopper, aphid and tetranychidae.

Description

Insecticidal composition

Technical Field

The present invention relates to an insecticidal composition which is highly effective against animal pests.

Background

The most typical problem in animal pest control is the need to reduce the dosage rate of the active ingredient to reduce or avoid adverse environmental or toxicological effects, while still providing effective animal pest control.

Another difficulty in pest control is that repeated and exclusive use of a single pesticidal active ingredient leads in many cases to rapid selection of pests that either naturally or acclimatize to resistance to the active ingredient.

Due to the multi-generation and adaptability of pests, the resistance of pests is formed in a rather short time. There is also a need for a pest control agent having an effect on both pests and their larvae and eggs, which has superior penetration.

In addition, in pest control, there is also a need to combine knockdown activity with prolonged control time, i.e., rapid action and long-lasting action.

Active ingredients of the formula I are known from WO 2007/144100. The insecticidal/acaricidal efficacy and/or the activity spectrum and/or the compatibility with plants (in particular with crop plants) of the known compounds is not always sufficient.

Disclosure of Invention

It is an object of the present invention to provide a pesticidal composition that solves at least one problem such as improving biological properties, providing synergistic properties, reducing dosage rates, expanding the spectrum of activity, combining knock-down activity with long-lasting action, management of resistance to drugs to delay resistance.

The present invention has unexpectedly found that the combination of a compound of formula I with a compound II selected from one of trifluorobenzene pyrimidine, thiocyclam, hydrazine depressant, bromoxynil fluorobenzdiamide, is effective in preventing or controlling animal pests and their larvae and eggs. Surprisingly, it has been found that the simultaneous (i.e. joint or separate) application of the active compounds compound of formula I and compound II or the sequential application of compound of formula I and compound II gives an enhanced pest control effect compared to the control rate possible with the individual compounds, this unexpected effect being a true synergistic effect, not just a sum of the activities.

The invention relates to an insecticidal composition which is realized by adopting the following technical scheme:

a pesticidal composition comprising as active ingredients a compound of formula I and a compound II selected from one of the following compounds: trifluoro-benzene pyrimidine, thiocyclam, hydrazine inhibitor and bromofenoxanil.

The compound II is selected from trifluoro-benzene pyrimidine.

The compound II is selected from thiocyclam.

The compound II is selected from the group consisting of a prohibiting hydrazine.

The compound II is selected from brofenpyrad fluorobenzene bisamide.

The weight ratio of the compound of formula I to the compound II is 50:1 to 1:300, preferably 25:1 to 1:200, preferably 10:1 to 1:150, more preferably 10:1 to 1:100, more preferably 10:1 to 1: 50.

The weight ratio of the compound of formula I to the compound II according to the invention may also be, for example, 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 3:1, 2:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:13, 1:15, 1:18, 1:20, 1:25, 1:28, 1:30, 1:35, 1:40, 1:45, 1:50, 1:55, 1:60, 1:65, 1:70, 1:75, 1:80, 1:85, 1:90, 1:95, 1:100, 1:120, 1:150, 1:160, 1:180, 1:200, 1:220, 1:250, 1:280, 1: 300.

A pesticidal composition comprising the active compounds formula I compound and compound II, the weight of the compounds of formula I and compound II together making up from 1% to 90%, preferably from 5% to 80%, more preferably from 5% to 70%, still more preferably from 5% to 60%, more preferably from 5% to 50% by weight of the pesticidal composition.

A pesticidal composition, which contains the active compounds a compound of formula I and a compound II, the weight of the compounds of formula I and compound II together making up 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% by weight of the pesticidal composition may also be, for example.

An insecticidal composition comprising as active ingredients a compound of formula I and a compound II, said insecticidal composition further comprising a surfactant and/or a filler.

The insecticidal composition provided by the invention is in the dosage form of missible oil, aqueous suspension, oil suspension, seed treatment dry powder, seed treatment suspension, water dispersible granules, wettable powder, suspoemulsion, aerosol, coated granules, extruded granules, aqueous emulsion, microcapsule suspension-suspension, dry suspension, ultra-low volume liquid, electrostatic oil, gel, granules and fine granules.

The insecticidal composition of the present invention may be applied undiluted or diluted with water.

The pesticidal compositions according to the invention surprisingly exhibit an extremely high activity in the treatment of plants, propagation material of plants, soil in which plants grow, in the prevention or control of pests and their larvae and eggs.

The insecticidal composition of the present invention has excellent control efficacy against pests and their larvae and eggs at low doses.

In another aspect, the invention also provides the use of a pesticidal composition for the prevention or control of pests and their larvae and eggs.

In particular, the invention provides application of the insecticidal composition in preventing or controlling pests of the families of snout moth, plutella, noctuidae, diabrotica, planthopper, aphididae and tetranychidae.

More particularly, the present invention provides the use of an insecticidal composition for preventing or controlling tobacco aphid, cotton aphid, peach aphid, diamond back moth, beet armyworm, spodoptera frugiperda, cotton bollworm, chilo suppressalis, rice leaf roller, phyllotreta striolata, ape worm, ape leaf worm, two-spotted leaf mite pests and larvae and eggs thereof.

Use of the pesticidal composition according to the invention for treating seeds to prevent the seeds from being attacked by pests.

The pesticidal composition according to the present invention may be applied to seeds, plants or fruits of plants or soil where plants grow or soil suitable for plant growth.

In another aspect, the invention provides a method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the invention to a target useful plant, a target pest or its environment, propagation material of a target useful plant. The plant propagation material is a seedling, rhizome, nursery seedling, cutting or seed.

A method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the present invention to seeds, target useful plants or soil in which plants grow or soil suitable for plant growth.

A method for preventing or controlling pests and their larvae and eggs, by applying the pesticidal composition according to the invention to the soil before, after or before germination of the seeds and/or directly to the soil in contact with the roots of the plants or to the soil suitable for the growth of the plants.

A method of protecting seeds comprising contacting the seeds before sowing and/or after pregermination with a pesticidally effective amount of the pesticidal composition of the invention.

The plant comprises stems and leaves of the plant, flowers of the plant, fruits of the plant and the like.

The seed is selected from potato, sunflower, coffee, tobacco, canola, rape, sugar beet, tomato, cucumber, bean, brassica, onion, soybean, wheat, barley, rye, oat, sorghum, peanut, sugarcane, rice, cole, cabbage, cowpea, carrot, cotton and corn seed.

A method for preventing or controlling pests comprising administering a compound of formula I, compound II, separately, sequentially or simultaneously.

The pesticidal composition according to the invention can be applied in any desired manner, for example seed coating, soil drenching and/or direct furrow application and/or foliar spraying, and before, after or before germination.

The invention relates to an insecticidal composition containing active ingredients of a compound shown in a formula I and a compound II, which solves at least one problem such as improvement of biological characteristics, provision of synergistic characteristics, reduction of dosage rate, enlargement of activity spectrum, combination of knockdown activity and long-acting effect, and resistance management to delay resistance.

The combination of a compound of formula I with a compound II is effective in preventing or controlling animal pests and their larvae and eggs. The simultaneous (i.e. joint or separate) application of the active compounds compound of formula I and compound II or the sequential application of compound of formula I and compound II gives an enhanced pest control effect compared to the control rate possible with the individual compounds, this unexpected effect being a true synergistic effect, not just a summation of the activities.

Detailed Description

For the purpose of facilitating an understanding of the present invention, the present invention will now be described by way of examples. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

The invention discovers that: the combination of the compound of formula I and the compound II is effective in preventing or controlling pests and their larvae and eggs. Surprisingly, the pest-controlling activity of the insecticidal compositions of the present invention is much higher than the sum of the properties of the individual active compounds. This unexpected effect is a true synergistic effect, not just a summation of activities.

The term "synergistic" as used herein means that the pesticidal effect of the active compound combinations or compositions of the present invention is greater than the sum of the effects of the individual active compounds, or that the effect is superadditive.

The invention provides a pesticidal composition which contains active compounds, namely a compound shown as a formula I and a compound II, wherein the compound II is selected from one of the following compounds: trifluoro-benzene pyrimidine, thiocyclam, hydrazine inhibitor and bromofenoxanil.

The insecticidal composition contains a compound shown in the formula I and trifluoro-benzene pyrimidine.

The insecticidal composition comprises a compound shown in the formula I and thiocyclam.

The insecticidal composition contains a compound shown in the formula I and a hydrazine inhibitor.

The insecticidal composition contains a compound shown in the formula I and bromofenoxanil.

The more preferred specific composition ratio is as follows:

in the insecticidal composition, when the compound II is selected from trifluorobenzene pyrimidine, the ratio of the compound I to the compound II is preferably 15:1-1: 15; more preferably 10:1 to 1:10, still more preferably 5:1 to 1: 5.

In the insecticidal composition, when the compound II is selected from thiocyclam, the ratio of the compound I to the compound II is preferably 10:1-1: 50; more preferably 5:1 to 1:40, more preferably 1:1 to 1:30, more preferably 1:1 to 1: 25.

In the insecticidal composition, when the compound II is selected from the group consisting of the food hydrazine inhibitor, the ratio of the compound I to the compound II is preferably 5:1-1: 100; more preferably 1:1 to 1:50, more preferably 1:1 to 1:25, more preferably 1:1 to 1: 10.

In the insecticidal composition, when the compound II is selected from the brofenpyrad, the ratio of the compound I to the compound II is preferably 15:1-1: 15; more preferably 10:1 to 1:10, still more preferably 5:1 to 1: 5.

The preparation of compounds II and their action on pests is known. Is disclosed in the website httpp:// www.alanwood.net/pesticides.

The total amount of active ingredients in the pesticidal compositions of the present invention may be selected to achieve the desired effect, depending on the particular factors. Such as dosage form, subject to be administered, method of administration, and the like.

The invention provides an insecticidal composition, which contains active compounds, namely a compound shown in formula I and a compound II, wherein the weight of the compound shown in formula I and the compound II accounts for 1% -90%, preferably 5% -80%, more preferably 5% -70%, still more preferably 5% -60%, and more preferably 5% -50% of the weight of the composition.

A pesticidal composition, which contains as active ingredients a compound of formula I and a compound II, the weight of the compounds of formula I and compound II together also accounting for, by weight of the pesticidal composition, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% may, for example, be added.

The pesticidal composition of the present invention may optionally comprise auxiliary ingredients such as agriculturally acceptable surfactants and/or fillers. Preferably, the auxiliary component is one or a mixture of a dispersant, a wetting agent, an antifreeze agent, a thickening agent, an antifoaming agent, a disintegrating agent, a binder and an auxiliary carrier.

According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the bulking agent is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.

The inactive fillers that can be used in the present invention can be either solid or liquid.

Examples of solid fillers that can be used are: examples of the inorganic filler include plant powders (for example, soybean powder, starch, cereal flour, wood flour, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, particles of corn cob and tobacco stalk, and residue after extraction of plant essence), clays (for example, kaolin, bentonite, and acidic china clay), talc powders, silicas (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, and calcium silicate), activated carbon, natural minerals (pumice, attapulgite, zeolite, and the like), and calcined diatomaceous earth.

The liquid filler that can be used is selected from water, isopropyl alcohol, butyl alcohol, ethylene glycol, propylene glycol, ethyl acetate, methyl oleate, amides, lactams (e.g., N-methylpyrrolidone) dimethyl sulfoxide, mineral and vegetable oils, and the like.

Surfactants which can be used for emulsifying, dispersing, solubilizing and/or wetting the active ingredient compounds include, for example, fatty alcohol polyoxyethylene ethers, polyoxyethylene alkylaryl ethers, polyoxyethylene higher fatty acid esters, phosphoric acid esters of polyoxyethylene alcohols or phenols, fatty acid esters of polyhydric alcohols, naphthalenesulfonic acid polymers, lignosulfonates, comb-like copolymers of high molecules, butylnaphthalenesulfonates, alkylarylsulfonates, sodium alkylsulfosuccinates, fats and oils, condensates of fatty alcohols with ethylene oxide, polyacrylates of alkyltaurates, protein hydrolysates, suitable oligosaccharides or polymers, based, for example, on vinyl monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.

For dispersing, stabilizing and adhering the active ingredient compounds, auxiliary agents such as xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate, natural phospholipids (such as cephalin and lecithin), synthetic phospholipids, bentonite, sodium lignin sulfonate and the like can be used.

Wherein the antifreezing agent can be selected from ethylene glycol, propylene glycol, glycerol, and sorbitol. As the deflocculant for the suspendable product, an auxiliary such as a naphthalenesulfonic acid polymer, a polymeric phosphate, or the like can be used. As the defoaming agent, a silicone defoaming agent can be used. In order to improve the flowability of the solid product, auxiliaries such as paraffin, stearate, alkyl phosphate and the like can be used.

Colorants which may be used, for example, inorganic pigments such as iron oxide, titanium oxide and prussian blue; and organic pigments/dyes, such as alizarin dyes, azo dyes, and metal phthalocyanine dyes; and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts.

Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.

The pesticidal composition according to the invention can be used as such or in the form of its formulation or in the use form prepared therefrom according to its respective physical and/or chemical properties, such as aerosols, microcapsule suspensions, cold fogging formulations, hot fogging formulations, microcapsule granules, fine granules, suspensions for seed treatment, ready-to-use solutions, powders, emulsifiable concentrates, oil-in-water emulsions, water-in-oil emulsions, macrogranules, microgranules, oil-dispersible powders, oil suspensions, oil solutions, foams, pastes, seed coatings, colloidal suspensions, suspoemulsions, aqueous solvents, suspensions, wettable powders, soluble powders, dusts and granules, water-soluble granules or tablets, water-soluble powders for seed treatment, wettable powders, natural and synthetic substances impregnated with active ingredients, microcapsules in polymeric materials and seed-coating substances, and ultra-low-volume cold fogging and hot fogging formulations.

The preferred preparation formulation of the insecticidal composition provided by the invention is missible oil, aqueous suspension, oil suspension, seed treatment dry powder, seed treatment suspension, water dispersible granules, wettable powder, suspoemulsion, aerosol, coated granules, extruded granules, aqueous emulsion, microcapsule suspension-suspension, dry suspension, ultra-low volume liquid, electrostatic oil, gel, granules and microgranule.

The pesticidal compositions of the present invention may also be applied in combination with other active ingredients such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like.

The formulations according to the invention can be prepared by mixing the active ingredients with the customary additives in a known manner. Such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents, if desired siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

The pesticidal composition of the present invention includes not only a composition which can be applied to a subject to be treated immediately by means of a suitable apparatus such as a spraying or dusting apparatus, but also a concentrated commercial composition which is diluted before application to the subject.

The insecticidal composition of the present invention can be prepared into a formulation suitable for application in the form of a combination of its active ingredient compounds, or can be applied in the form of a combination of commercial formulations in appropriate dosages.

The pesticidal compositions according to the invention, which have good plant compatibility and favourable warm-blooded animal toxicity, are suitable for controlling pests encountered in agriculture, forestry, the protection of stored products and materials and in the hygiene sector, in particular Insecta (Insecta), Arachnida (Arachnida), Nematoda (Nematoda). They are active against normally sensitive and resistant species and against all or individual developmental stages, and also achieve a surprising "synergistic" effect in preventing or controlling pests.

The invention also provides a use of the insecticidal composition of the invention for preventing or controlling pests. The term "pest" is used herein to mean any organism that can cause loss of the normal state of a plant. Including Insecta (Insecta), Arachnida (Arachnida), and Nematoda (Nematoda).

The Insecta includes Lepidoptera, Coleoptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Thysanoptera, Isopoda, Diplopoda, Symphyta, Thysanoptera, Rhamnoidea, Orthoptera, Dermaptera, Isoptera, and Anoplura.

Lepidopteran pests: the families of the borer moth (Pyralidae) such as chilo suppressalis (chilblains (walker), tryporyza incertulas (walker), cnaphalocrocis medinalis guene (cnaphalocrocis medinalis), cabbage borer (hellulaundalis), peach borer (Conogethespunctiviris), the families of the whiteflies (Pieridae) such as Pieris rapae (Pieriis rapae), Pieris citrifolia (papilioxutatus), white butterfly (Pierisrapaeecruscula), Orthosiphon oryza sativa (pararaguttata); arctidae (Arctidae) such as fall webworm (Hyphantriacanea); noctuidae (Noctuidae) such as Trichoplusia ni (trichoplusiani), Trichoplusia brassicae (mamestrabarasica), Spodoptera exigua (spodopterageua), Spodoptera litura (spodoptera litura), Heliothis armigera (heliotropigeria), Oriental armyworm (pseudolitea seprate), and Tiger (Agrotis ipsilon); tortricidae (Tortricidae) such as tea leaf moth (adoxyphesoranafiasta), apricot leaf roller moth (Archipsfussciureus), tea leaf roller moth (homoamagnanima); diamondback moth (Plutellidae) such as diamondback moth (plutella xylostella); the family of the Gelechiaceae (Gelechiaceae) such as Helicoverpa armigera (petitophopogossypiella), Helicoverpa zea (Pink bollworm);

coleopteran pests, for example: rice weevil (mitophilis oryzae) citrus leaf beetle (Podagricomelanicolis Chem), maize weevil (S. zeamais), cereal weevil (S. granatus), gibbonella ape (Cabbegeleaf beetle), ape leaf beetle (Daikon leaf beetle), flea beetle (flabellole), grape flea beetle (Aliphalybia), striped flea beetle (Phyllotreta), cucumber flea beetle (Epitricucurumeris), tobacco flea beetle (Ehirtipipennis), leaf beetle (E. fuscula), yellow melon (Australia purpurea (Gemlining), leaf beetle mustard (Phaecochleariae), rice water weevil (Lissopterus orientalis), yellow melon (yellow rice tuber), yellow rice leaf beetle (yellow rice), yellow rice leaf beetle (yellow beetle), yellow beetle (yellow beetle) (yellow beetle), yellow beetle (yellow beetle), yellow beetle (yellow beetle ) (yellow beetle, yellow beetle (yellow beetle, yellow beetle;

diptera (Diptera), for example, Aedes spp, Anopheles spp, Geotrichum japonicum, Blastomyelia rubra (Calliphorochella), Ceratoides mediterranei (Ceratoptera capitata), Chrysomyelia spp, Conoidomyelia spp, Erythromyces spp, Cordylobia anthropogata, Culex spp, Flavodia sp, Erythrocystomyelia rugosa (Dacus olepis), Dermatobia hominis, Drosophila spp, Oesophagostomyelia spp, Meloidogyne spp, Ostrinia spp, Meloidogyne spp, Meloidogp, Stinging flies (Stomoxys spp.), Tabanus (Tabanus spp.), Tannia spp., European midge (Tipula paludosa), and Phryptodermis (Wohlfahria spp.).

From the order of the Heteroptera (Heteroptera), for example, Dinopecten moschatus (Anasa tristis), Triphoides sp, Orthosiphon (Blissus spp.), Orthosiphon (Caloris spp.), Camphyloma livida, Allium dorsalis (Camphorus spp.), Thorosiphon (Camphorus spp.), Orthosiphon (Camphorus spp.), Symphyla (Camelpholus spp.), stinkbug (Cimex spp.), Creutides dilutus, Piptosiphon (Dasynus Pieris), Dichelops furcata, Orthosiphon (Diocopterus stinoruslygus), Euschistus (Symphus spp.), Euschistus sp., Euonyx japonicus, Euonymus fortunei (Phyllocus spp.), Pirophus spp (Pirophus spp.), Piroplus spp Theobroma cacao (Sahlbergella singularis), lygus (Scotinophora spp.), corilagopus (Stephanitis nasi), Tibracha spp, Nephocorium (Triatoma spp.).

Homoptera (Homoptera), for example, Elaphytis gracilis (Achythospon spp.), Aenolomia spp, Elaphania louse (Agonospora spp.), pediculopsis sp, Aleurolobus sp, Aleurothrix spp, Amrasca spp, Anurapia caris, Elaphania spp (Aonidia spp.), Solidago virginica (Aphanostigma), Aphis virginosa (Brushis spp.), Arboridia cicadae (Arboridia indica), Aphis peltata (Aspidiella spp.), Aphis virginosa (Aphanizomepidida), Phyllophora viridis (Brachypoda), Phyllophysodaria viridis (Brachypodocarpus), Phyllophycus viridis (Lepidorhiza), Phyllophycus carotoviridis (Lepidorhiza), Phytophagoides sp), Phytophagoides sp, Phytophaga viridis (California), Phytophagoides sp), Phytophagoides sp (Phoma viridis (Brachys, Phytophagoides), Phytophagoides sp (Phoma viridis (Pholiota viridis (Callica, Phymatopsis (Leonicola, Phymatopsis (Lespera) and Phymatopsis (Lespe, Phytophagoides sp), Phymatopsis (Lespera virids (Blastoma viride (Blanconicola (Blastus spp) The plant diseases include, but are not limited to, the plant species diaphorus (chlamydia), phaedodes melanophora (chlamydius ficus), corn leafhopper (cicadolina mbila), cocomytus villi, lecithium (coculus spp), cryptophycus virens (cryptophys ribis), dalbumus spp, dialeuroides spp, Diaphorina spp, leucococcum spp (diasporium spp), dolalis spp, drospiria spp (drosophila spp), cervus spp, calophyllum spp (trichophys spp), calophyllum spp (trichophyceae spp), calophyllum spp (calophyllum spp), phaedo spp) Mahanarva fimbriola, sorghum aphid (Melanaphus sacchara), Metalfiella spp, Metalforhodopsis (Metholophum dirhodum), Aphis nigricans (Monilia costalis), Ozunophilus Oncorhyni (Myzus spp.), Physalis lactuca (Nasonophora rubisnigri), Phytophthora nigra (Nephotettix spp.), Bemisia myrica (Parabellaria myrica), Pedunculus sp (Paralichia spp.), Physalis sp, Phymatophora sp, Pemphigus sp, Cercosphaera (Peregrius), Phytophagus purpureus (Phyllophora spp.), Populus planus (Phyllophora spp.), Phosphaera sp), Phytopira palustaria (Phyllophora sp), Phytopira palustaria sp), Phytophagus sp (Phyllophora sp), Phytophagoides sp (Phyllophora sp), Phyllophora sp (Phyllophora spp), Phyllophora spp Quesada gigas, Fasciola spp, Sinomonas spp, Sinonospora spp, Saissetia spp, Scaphoides titatus, Schizaphis graminum, Pseudobulbus ferulae, Pectinopus elongatus, Sogata spp, Sogatella furcifera, Nilaparvata, Rhapotheca laticeps, Toxoplasma spp, Pholiopsis aurantiaca, Trialeuroporus viridis, Rhapophora virida viridis, Rhapophora vitis, grape nodule, grape seed, grape.

From the order of the Hymenoptera (Hymenoptera), for example, from the genus Trichoplusia (Diprion spp.), from the genus Vespa (Hoplocpa spp.), from the genus Trichoplusia (Laius spp.), from the genus Melissa (Monomorium pharaonis), from the genus Vespa (Vespa spp.).

From the order of the Orthoptera (Orthoptera), for example, cricket (Acheta domesticus), Blatta orientalis (Blatta orientalis), Blattella germanica (Blattella germanica), Gryllotalpa spp, Matdra (Leucophaea maderae), Locusta (Locusa spp.), Locusta nigricans (Melanoplus spp.), Periplaneta americana (Periplaneta americana), Locusta desert (Schistocerca gregaria), and Acridae (Acridae); stinkbug (megatiptatticimum), corium elephatis (eurydemasum), stinkbug (eusartorius major), stinkbug (eusarcocylilissis), stinkbug (eusarcocorisparvus), stinkbug (nezaraviridula), stinkbug (platistali), stinkbug (halirphista), stinkbug (clethostachydigitalis), stinkbug (clethostus punctiger), stinkbug (lygus lucentis), stinkbug (leptospiensis), stinkbug (sterrisitis), and stinkbug (gonotylupestium).

From the order of the Thysanoptera (Thysanoptera), for example, Thrips oryzae (Balothrips biformis), Enothrips flavens, Frankliniella spp, Silybum spp, Philithrips spp, Hercinothrips viridans (Hercinothrips femoralis), Thrips cardboards (Kakothrips spp.), Thrips vitis (Rhipicrophorus cruentus), Thrips harderii (Sciroththrips spp.), Taeniothrips cardamoni, Thrips spp.

From the order of the Isoptera (Isoptera), for example, Reticulitermes spp, Termite spp.

From the order of the Thysanura, for example, Chlamydomonas (Lepisma saccharana).

From the order of the louse (Anoplura, Phthiraptera), for example, the genera zoophthiridae (Damalinia spp.), Mediculus (Haematopinus spp.), Phthirius (Linogaphus spp.), Phthirius (Pediculus spp.), and Phthirius (Trichodectus spp.).

Insects of the order Acarina (Arachnida). For example, Panonychus citri (pannyhuscirri), Tetranychus cinnabarinus (tetranyhuscina), Panonychus ulmi (pannyhusunimi), Tetranychus urticae (tetranyhusuntica), Tetranychus urticae (tetranyhusnensis), phyllopodium leptorum (oligonyhusunuus), phyllopodium citrosum (eotropicalis), brevipisporus purpureus (breviperuricius), phyllopodium tritici (acarus tritici), phyllopodium humicola (british purpurea), phyllopodium tritici (americanus), phylloporus tritici (americanus), vitiligo chinensis (acarus vitis), phylloporus sinensis (caraganus carinatus), phyllus gallinae (Tetranychus), phylloxerussimus (polygordonus), phylloruslatus (Tetranychus), Tetranychus (Tetranychus), etc.

Bivalve molluscs (Bivalva), for example, lobium (Dreissena spp.).

From the order of the Chilopoda (Chilopoda), for example, Geophilus spp.

From the order of the Collelmola (Collelmbola), for example, Onychiurus armatus (Onychiurus armatus).

From the Dermaptera (Dermaptera), for example, Forficula auricularia (Forficula auricularia).

From the order of the Diplopoda (Diplopoda), for example, Blaniulus guttulatus.

Gastropoda (Gastropoda), for example, Arion spp, hemifusus (biomhalaria spp), paulospira (Bulinus spp), hemifusus (deraceras spp), hemifusus (Galba spp), lymerus (Lymnaea spp), Oncomelania (Oncomelania spp), and hemifusus (Succinea spp).

Isopoda (Isopoda), for example, Armadillidium vulgare (Armadillidium vulgare), Onychium pectinosum (Oniscus asellus), Armadillidium globosum (Porcellio scaber).

Siphonaptera (Siphonaptera), for example, Ceratophyllus spp (Ceratophyllus spp.), Xenopsylla cheopis (Xenopsylla cheopis).

The synthetic mesh (Symphyla), for example, white pine worm (Scutigerella immaculata).

Representative organisms of the class Nematoda (Nematoda) are selected from the group consisting of Meloidogyne spp, Heterodera spp, Globodera spp, Heterodera spp, Brevibacterium spp, and Longidorus spp. Preferred are soybean cyst nematode (Heterodera), potato nematode (Golbodera roseochiensis), Meloidogyne incognita (Meloidogyne incognita), and the like, which are the following nematodes.

In particular, the insecticidal composition of the present invention shows a surprising "synergistic" effect on the prevention or control of borer moth, plutella, noctuidae, diabrotidae, planthopper, aphid, tetranychidae pests and their larvae and eggs.

The family of the borer moth (Pyralidae) such as chilo suppressalis (chilblains (walker)), tryporyza incertulas (walker), cnaphalocrocis medinalis guene (cnaphalocrocis medinalis), cabbage borer (hellullaundalis), dichocrocis punctiferalis (Conogethe spiculations);

noctuidae (Noctuidae) such as Trichoplusia ni (trichoplusiani), Trichoplusia brassicae (mamestrabarasica), Spodoptera exigua (spodopterageua), Spodoptera litura (spodoptera litura), Heliothis armigera (heliotropigeria), Oriental armyworm (pseudolitea seprate), and Tiger (Agrotis ipsilon);

diamondback moth (Plutellidae) such as diamondback moth (plutella xylostella);

aphid family: such as Myzus persicae (Myzus persicae), cotton aphid (Aphis gossypii), cabbage aphid (brevicornus brassicae), tea deer cryptic frontal aphid (Cryptomyzus rivis), broad bean aphid (Aphis fabae), apple aphid (Aphispomi), apple cotton aphid (eriosma lanigerum), peach tail aphid (hyalopterusarinis), grape root nodule aphid (Phylloxera vastatrix), ductus avenae (macrophyllus avenae), ductus avenae (metaplophyum dirhodium), ductus graminearus avenae (Rhopalosiphum padi), and wheat aphid (schizophritis grandis gramininum);

plant hopper family: laodelphax striatellus, Nilaparvata lugens (Nilaparvata lugens), Sogatella furcifera, Hexaparina saccharea (Perkinsiellaceae);

family phyllotaceae: phyllotreta striolata (Phyllotreta striolata), Zaocys cucurbita (Aulacophora femoralis), Bullenia apetala (Cabbageleaf), Bullenia apetala (Daikon leaf bean);

tetranychidae: tetranychus urticae (pannychus cinnabarinus), Tetranychus cinnabarinus (Tetranychus cinnabarinus), Tetranychus ulmi (pannychus ulmi), Tetranychus urticae (Tetranychus urticae), Tetranychus viennensis (Tetranychus viennensis), Tetranychus citrullus kankis (eotarranychus kankittus), and Tetranychus tritici semen Tritici aestivi (Aceriaculatus).

More particularly, the pesticidal combinations of the present invention are particularly effective in preventing or controlling pests such as tobacco aphid, cotton aphid, peach aphid, diamond back moth, beet armyworm, spodoptera frugiperda, cotton bollworm, chilo suppressalis, rice leaf roller, phyllotreta striolata, ape beetle, tetranychus urticae koch, tetranychus citri, tetranychus cinnabarinus and the like, and larvae and eggs thereof.

The insecticidal composition of the invention surprisingly exhibits very high activity in the treatment of plants, propagation materials of plants, soil in which plants grow, in the prevention or control of pests of the families of snout moth, plutella, noctuidae, diabrotica, planthopper, aphididae, tetranychidae and their larvae and eggs.

The insecticidal composition has excellent control efficiency on pests of the families of snout moth, plutella, noctuidae, phyllanthaceae, plant hopper, aphididae and tetranychidae and larvae and eggs thereof at low dosage.

In another aspect, the invention also provides the use of an insecticidal composition for preventing or controlling borer moth, plutella, noctuidae, diabrotica, planthopper, aphid, tetranychidae pests and their larvae and eggs.

The invention provides an application of an insecticidal composition in preventing or controlling tobacco aphid, cotton aphid, peach aphid, diamond back moth, beet armyworm, spodoptera frugiperda, cotton bollworm, chilo suppressalis, cnaphalocrocis medinalis guenee, phyllotreta striolata, great ape leaf, small ape leaf worm, two-spotted leaf mite pest and larva and eggs thereof.

The pesticidal compositions of the present invention may be applied to any and all developmental stages of a pest, such as eggs, larvae, pupae and adults. Pests can be controlled by contacting the target pest, its food supply, habitat, breeding grounds or their locus with a pesticidally effective amount of the pesticidal composition of the present invention.

"locus" refers to a plant, plant propagation material, soil, area, material or environment in which pests are growing or may grow.

"pesticidally effective amount" refers to the amount of the pesticidal composition of the present invention required to obtain an observable effect on growth, including a mortality, prophylactic or removal effect, a destructive effect or an effect that reduces the appearance and activity of animal pests. The pesticidally effective amount of each pesticidal composition used in the present invention may vary. The pesticidally effective amount of the composition will also vary depending on the prevailing conditions such as the desired pesticidal effect and duration, the weather, the target species, the locus, the mode of application and the like.

All plants and plant parts can be treated according to the invention. "plants" is understood here to mean all plants and plant populations such as desired and undesired wild plants or crops (including naturally occurring crops). Crops may be plants obtained by conventional breeding and optimization methods, or plants obtained by biotechnological and genetic engineering methods or combinations of these methods, including transgenic plants and including plant cultivars which may or may not be protected by plant breeders' certificates. Plant parts are understood to mean all parts and plant organs above and below the ground, such as shoots, leaves, flowers and roots, examples which may be mentioned being leaves, needles, stems, flowers, fruit bodies, fruits and seeds and roots, tubers and rhizomes. Parts of plants also include harvested plants and vegetative and generative propagation material, for example seedlings, tubers, rhizomes, cuttings and seeds.

As mentioned above, all plants and parts thereof can be treated according to the invention. In a preferred embodiment, wild plant varieties and plant cultivars, or those obtained by conventional biological breeding methods such as crossing or protoplast fusion, and parts thereof, are treated. In a more preferred embodiment, transgenic plants and plant cultivars obtained by genetic engineering (optionally in combination with conventional methods) and parts thereof are treated.

The insecticidal composition has good plant compatibility, and is suitable for controlling pests, particularly insects, mites and nematodes, as well as fungi, rodents, microorganisms and the like, particularly the pests of the families of snout moth, plutella, noctuidae, diabrotidae, planthopper, aphididae and tetranychidae. It is preferably applicable to foliar treatment, soil treatment and seed treatment.

In another aspect, the invention provides a method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the invention to a target useful plant, a target pest or its environment, propagation material of a target useful plant. This can be done before and after the plant, plant propagation material or environment is infested with pests.

The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.

The plant propagation material is a seedling, rhizome, nursery seedling, cutting or seed. Seeds are preferred.

The seed is selected from potato, sunflower, coffee, tobacco, canola, rape, sugar beet, tomato, cucumber, bean, brassica, onion, soybean, wheat, barley, rye, oat, sorghum, peanut, sugarcane, rice, cole, cabbage, cowpea, carrot, cotton and corn seed. Of particular importance is the treatment of wheat, barley, rye, oats, corn, cotton, canola, rape and rice seeds.

Accordingly, the present invention provides a method for protecting seeds, seedlings roots and shoots from attack by soil and foliar insects comprising contacting the seeds prior to sowing and/or after pregermination with an effective amount of the pesticidal composition of the present invention.

The invention also provides a method of controlling or preventing pests and their larvae and eggs in plant propagation material and plant organs, plant parts and/or plants which grow at a later time, which comprises applying the pesticidal composition of the invention to the plant propagation material or its environment. The plant propagation material is preferably a seed.

Most of the damage caused by pests to crop plants occurs as early as when the seeds are infested, during storage and after the seeds are sown into the soil and during or after germination of the plants. This stage is particularly critical because the roots and shoots of growing plants are particularly sensitive, and even minor damage can lead to death of the entire plant. Therefore, the protection of seeds and germinating plants by using suitable compositions is of particular interest.

The invention also relates in particular to a method for protecting seeds and germinating plants from attack by pests and their larvae and eggs by treating the seeds with the insecticidal composition according to the invention. The method of the present invention for protecting seeds and germinating plants from infestation by pests of the families of the borer moth, plutella, noctuidae, diabrotidae, planthopper, aphididae, tetranychidae and their larvae and eggs comprises a method for treating said seeds simultaneously with a compound of formula I and a compound II. It also includes a method of treating the seed with a compound of formula I and a compound II at different times.

A method for preventing or controlling pests and their larvae and eggs, by treating the seeds of the plants to be grown before sowing and/or after pregermination with a synergistically effective amount of the pesticidal composition according to the invention.

The invention also relates to the use of the pesticidal composition of the invention for treating seeds to protect said seeds and plants grown therefrom from attack by pests and their larvae and eggs.

Furthermore, the invention relates to seeds treated with the pesticidal composition of the invention to obtain protection against pests and their larvae and eggs.

The invention also relates to seeds treated simultaneously with a compound of formula I and a compound II. The invention also relates to seeds treated with a compound of formula I and a compound II at different times. For seeds treated with a compound of formula I and a compound II at different times, the respective active compounds of the pesticidal composition of the present invention may be present in different layers on said seeds. Wherein the layers comprising the compound of formula I and the compound II are optionally separated by an intermediate layer. The invention also relates to seeds in which the compounds of the formula I and the compounds II are applied as coating components or as one or more additional layers outside the coating.

The pesticidal composition of the present invention may be applied to seeds in any physiological state. Preferably, the seeds are in a sufficiently durable state so as not to be damaged during handling. Generally, the seeds may be seeds harvested from the field, removed from the plant, isolated from any cob, stem, husk and surrounding pulp or other non-seed plant material. The seed may also preferably be biologically stable to the extent that the treatment does not cause biological damage to the seed. The treatment applied to the seed may be between seed harvest and seed sowing or at any time during the sowing process. The seeds may also be pregerminated before or after treatment.

The seeds treated with the pesticidal composition of the present invention can be stored, managed, sowed and tilled.

Seed treatment can be by applying the compound of formula I and compound II thereto in any desired sequence or simultaneously.

Seed treatment occurs on unsown seeds, and the term "unsown seeds" is intended to include seeds at any time between harvest of the seeds and sowing of the seeds in the ground for the purpose of germination and growth of the plant. Preferably, the treatment occurs before sowing of the seeds, whereby the sown seeds have been pre-treated with the combination. In particular, seed coating or seed pelleting is preferred in the treatment of the combination of the invention. After treatment, the components of each combination adhere to the seed and are thus available for pest control.

Seed treatment methods include all methods known to the person skilled in the art which are suitable for treating seeds, such as seed dressing, seed coating, seed soaking, seed coating, seed multilayer coating, seed encrusting, seed wetting, seed dusting and seed pelleting.

The seeds treated with the pesticidal composition of the present invention provide protection from pest attack not only to the seeds themselves but also to the plants growing from the seeds after their emergence. Thus, it may not be necessary to treat the plants directly at the time of sowing or shortly thereafter.

The compositions of the present invention may be applied to seeds, plants or fruits of plants or soil where plants are growing or soil suitable for plant growth.

The present invention also provides a method for preventing or controlling pests and their larvae and eggs, comprising applying the pesticidal composition of the present invention to seeds, target useful plants or soil in which plants grow or soil suitable for plant growth.

In another aspect, the invention also provides a method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the invention to the soil before, after or before germination of the seeds and/or directly to the soil in contact with the roots of the plants or to the soil suitable for the growth of the plants.

The invention also provides a method of protecting plants from attack by pests and their larvae and eggs, which comprises applying the pesticidal composition of the invention to the environment, habitat or storage area where useful plants are growing. The environment and habitat for plant growth refers to a support capable of rooting and growing crops, such as: examples of the raw material include sand, pumice, vermiculite, diatomaceous earth, agar, gel, polymer, asbestos, wood chips, and bark. Preferably soil.

The invention also provides a method for protecting plants against infestation by pests of the families of the borer, plutella, noctuidae, diabrotidae, planthopper, aphididae, tetranychidae and their larvae and eggs, which comprises applying the active components compound of formula I and compound II jointly, separately or in succession. In the case of separate application, the sequence usually has no effect on the results of the control measures.

A method for preventing or controlling pests and their larvae and eggs, comprising applying a compound of formula I, compound II, separately, sequentially or simultaneously.

The present invention also provides a method for protecting useful plants from attack by pests and their larvae and eggs, which comprises applying a combination comprising a compound of formula I and a compound II, in any desired sequence or simultaneously, on the target useful plant or its environment, the target pest or its environment, propagation material of the target useful plant.

The insecticidal compositions of the present invention include not only ready-to-use compositions which can be applied to plants or plant propagation material with a suitable device, but also commercial concentrates which must be diluted with water prior to application.

The invention provides a method for preventing or controlling pests and their larvae and eggs, by applying the pesticidal composition of the invention to the target useful plants or their environment, the propagation material of the target useful plants, the other pesticides can be applied before, simultaneously with or independently of the compounds of formula I and II, and in the case of separate application of the compounds of formula I and II, each other pesticide can be applied before, simultaneously with, between or independently of the application of the compounds of formula I and II.

Methods for treating target pests, target useful plants, or seeds, soil with the pesticidal composition of the present invention include, for example, spreading treatment, soil treatment, surface treatment, and fumigation treatment. Spreading treatments include, for example, spreading, spraying, atomizing, particle application. Soil treatment includes, for example, soil irrigation and soil mixing. Surface treatments include, for example, coating, covering. Fumigation treatments include, for example, covering the soil with a polyethylene film after injection into the soil. The preferred direct treatment of the plants is a foliar application treatment.

Applicators typically use the insecticidal compositions of the present invention in a backpack sprayer, spray can, spray plane or irrigation system. The pesticidal compositions of the present invention are typically formulated with water, buffering agents and/or other adjuvants to the desired application concentration to give a ready-to-use spray.

Compared with the prior art, the invention has at least the following beneficial effects:

(1) the pesticidal compositions of the present invention may demonstrate synergistic activity compared to the activity of the compounds alone;

(2) the plants, plant propagation materials treated with the insecticidal composition of the invention reach a level which shows a significant improvement;

(3) the pesticidal composition of the present invention reduces any unwanted infestation and attack by pests and their larvae and eggs; in particular of the families of the borer, plutella, noctuidae, diabrotidae, planthopper, aphididae, tetranychidae and their larvae and eggs;

(4) the compound of the formula I and the compound II are mixed, so that the problems of persistence and quick action are solved, the medicine taking times are reduced, and the labor cost is reduced;

(5) the insecticidal composition of the present invention exhibits high efficacy, thereby reducing the dose rate and reducing the load on the environment;

biological test example

The expected effect of a particular combination of two active ingredients can be calculated using the so-called "Colby' formula" (see s.r. Colby, "marketing synergy and antibiotic Responses of pharmaceutical compositions", Weeds 1967,15, 20-22):

x is the control effect when using the active ingredient compound of the formula I in an amount of m g/ha or at a concentration of m ppm, expressed as a percentage of the untreated control,

y is the control effect when active ingredient compound II is used in an amount of n g/ha or at a concentration of n ppm, expressed as a percentage of the untreated control,

e is the control effect when using the active ingredients compounds of the formula I and compounds II in amounts of m and n g/ha or in concentrations of m and n ppm, expressed as a percentage of the untreated control,

if the actual control effect exceeds the calculated value, the lethal effect of the composition is superadditive, i.e. there is a synergistic effect.

Test example 1 cabbage aphid test

Preparing a liquid medicine: compounds of formula I (synthesized as described in WO 2007/144100), compound II in table 1, were dissolved in acetone to prepare single-dose stock solutions, which were diluted to the desired concentration with 0.1% tween-80 in water.

The method comprises the steps of taking the pink aphids (Myzus persicae Sulzer) as tested pests, collecting the tested insects from a cabbage vegetable field in the Kunshan test of Jiangsu, breeding a generation on an insect-free cabbage planted in an insect-proof net room, and selecting 30 adult pink aphids with similar body colors and uniform sizes as a group for indoor test.

Aphid and cabbage leafSoaking the slices in the prepared medicinal liquid for 10s, taking out, sucking off the excessive medicinal liquid with absorbent paper, and placing into a plastic beaker with diameter of 6cm and height of 10 cm. After the test is finished, the test is shifted to 25 +/-2^oC, feeding in a constant temperature incubator with the relative humidity of 60% -80%. Control treatment was blanked with treatment without agent. The test results were checked for 72h of drug treatment. If the control mortality rate is more than 20%, the test is redone.

The calculation method comprises the following steps:

mortality (%) = (number of living insects before medicine-number of living insects after medicine)/number of living insects before medicine x 100

Control effect% = ((treatment group mortality-control group mortality)/(100-control group mortality)) × 100

TABLE 1 test of combinations of Compounds of formula I and Compounds II on Nepeta persicae

Experiment 2 diamondback moth assay

Preparing a liquid medicine: the compound of formula I (synthesized by the method described in WO 2007/144100), compound II bulk drug in table 2 were dissolved in acetone to prepare a single-dose mother liquor, which was then diluted to the desired concentration with an aqueous solution containing 0.1% tween-80.

Diamondback moth ((Plutella xylostella Linnaeus), farmed population in eastern village of kunju kunshan, indoor experiments were performed with 10 heads as a group.

Soaking caulis et folium Brassicae Capitatae in the medicinal liquid for 10S, air drying, placing in glass culture dish (diameter 60 cm) filled with filter paper, moistening petiole with wet cotton ball, inoculating 3-year-old larva into leaf, sealing with preservative film for preservation, covering, placing in 25 + -1 container^oC, relative humidity of 60% -80%, illumination period L: d = (16:8) h in an incubator.

Control treatment was blanked with treatment without agent. The results were checked after being kept 72 in the incubator. The death judgment criteria were: the body of the worm is touched with a poking needle, and the worm body is dead without reaction or can not react normally.

The calculation method comprises the following steps:

TABLE 2 control of diamondback moth by combination of a compound of formula I and a compound II

Test example 3 Chilo suppressalis test

Preparing a liquid medicine: the compound of formula I (synthesized by the method described in WO 2007/144100), compound II bulk drug in table 3 were dissolved in acetone to prepare a single-dose mother liquor, which was then diluted to the desired concentration with an aqueous solution containing 0.1% tween-80.

Chilo suppessalis (Chilo suppessalis). Chilo suppressalis egg masses are collected from a farm in Kunlshan Oriental village of Jiangsu, and after indoor breeding for 2-3 generations, larvae at the middle stage of 2 years old are selected for testing.

Taking the rice seedlings which grow well, and sucking the surface water by using absorbent paper. Soaking in the prepared medicinal liquid for about 10 seconds, and naturally drying in the shade. The mixture was transferred into a test tube. Inoculating 10 heads/pipes of 2-instar larvae of Chilo suppressalis, and plugging with cotton. Is placed at 25 +/-1^oC, relative humidity of 60% -80%, illumination period L: d = (16:8) h in an incubator. Control treatment was blanked with treatment without agent. After 4d the test results were checked. Failure to crawl normally is considered dead worms.

If the control mortality rate is more than 20%, the test is redone.

The calculation method comprises the following steps:

TABLE 3 prevention of Chilo suppressalis by combination of Compounds of formula I and II

Test example 4 test of rice leaf rollers

Preparing a liquid medicine: the compound of formula I (synthesized by the method described in WO 2007/144100), compound II in table 4, was dissolved in acetone to prepare a single-dose stock solution, which was then diluted to the desired concentration with an aqueous solution containing 0.1% tween-80.

The rice leaf roller (Cnaphalocrocis medinalis) is obtained by collecting larva from the farm in Kunloshan village of Jiangsu, and breeding 3 generation of 3-year-old test insects with rice seedlings indoors.

Soaking fresh and tender rice seedlings in a test solution for 10s, taking out and putting into a prepared glass test tube, naturally drying, inoculating 10 heads of 3-instar rice leaf folder larvae into the test tube, fastening the tube opening with gauze, and culturing in an observation room after treatment.

Control treatment was blanked with treatment without agent. After 72h of treatment, the rice stems are peeled off to check the death condition of the test insects, and the death condition is recorded. The judgment standard of the death of the test insects is as follows: death was counted as no response when the test insects were gently touched by tweezers. If the control mortality rate is more than 20%, the test is redone.

The culture conditions of the test target and the target after the test were 25. + -.1^oC, relative humidity of 60% -80%, illumination period L: d = (16:8) h constant temperature incubator.

The calculation method comprises the following steps:

TABLE 4 controlling effect of combination of compound of formula I and compound II on cnaphalocrocis medinalis

Test example 5 brown planthopper test of rice

Preparing a liquid medicine: the compound of formula I (synthesized by the method described in WO 2007/144100), compound II in table 5, was dissolved in acetone to prepare a single-dose stock solution, which was diluted to the desired concentration with an aqueous solution containing 0.1% tween-80.

Brown planthopper (Nilaparvata lugens stal) is collected from farm rice experimental field in Kunzshan Oriental village in Jiangsu, and bred with rice seedlings indoors.

The temperature of the insect-raising room is 28 +/-2^oC, the relative humidity is above 70%, and the lamp is irradiated for 14 hours every day. After accelerating germination of rice seeds, sowing the rice seeds in a plastic box with the length of 20cm multiplied by 30cm, and feeding rice planthopper nymphs to adults when rice seedlings grow to be more than 10 cm. Placing potted rice plants without rice planthopper eggs into an insect-raising net cage with the length of 30cm multiplied by 50cm, inoculating egg-holding female adults to lay eggs for 2 to 3 days, taking out rice seedlings, and replacing the rice plants without the egg-holding eggs in the insect-raising box. After the brown planthopper eggs on the rice plants taken out from the insect breeding box are hatched, 3-day-old brown planthopper nymphs are taken for testing each time.

Selecting rice seedlings cultivated in a greenhouse, digging the rice seedlings with roots, cleaning the rice seedlings, cutting the rice seedlings into rice stems which are about 10cm long and have a few root hairs, and airing the rice stems in a shade until no water mark exists on the surfaces of the rice stems for later use. Soaking the prepared rice stem in the medicinal liquid for 30 s, taking out, air drying, and placing in 3cm × 20cm test tubes with 3 pieces per tube. The test tube was sealed with black cloth and covered with a wet towel to moisturize.

The test insects are transferred into test tubes by a trematode device, 15 ends of each test tube are covered by gauze. The treated test insects are placed at the temperature of 25 +/-1^oC, the relative humidity is 60% -80%, and the illumination period is L: d = (16:8) h feeding and observation. The test insects are treated for 48h to check the death condition and record. The judgment standard of the death of the test insects is as follows: death was counted as no response when the test insects were gently touched by tweezers. If the control mortality rate is more than 20%, the test is redone. Control treatment was blanked with treatment without agent.

The calculation method comprises the following steps:

TABLE 5 control of brown planthopper by combination of compound I and compound II

Test example 6 phyllotreta striolata test

Preparing a liquid medicine: the compound of formula I (synthesized by the method described in WO 2007/144100) and the compound II of table 6 were dissolved in acetone to prepare single-dose mother solutions, which were diluted to the desired concentration with an aqueous solution containing 0.1% tween-80.

Phyllotreta striolata (Phyllotreta striolata) is used as a test pest and collected from one vegetable field of a farm in Kunzshan Oriental village of Jiangsu. Before the test, phyllotreta striolata is inoculated on greenhouse potted cabbage planted in an insect-proof net room of a laboratory. After continuously feeding for 72h, selecting phyllotreta striolata adults with consistent size for testing. Laboratory tests were performed on a 20-head set.

Soaking cabbage leaves which are not polluted by the medicament in the test liquid for 15s, taking out, naturally drying, placing in a glass bottle, inoculating 20 heads of phyllotreta striolata adults with the same size, and sealing with fine gauze. After the test is finished, the test is shifted to 25 +/-2^oC, feeding in a constant temperature incubator with the relative humidity of 70% -80%. The control treatment was blanked with clear water without drug. The test results were checked 48h after the treatment with the drug. The dead insect was identified as the non-response of the brush pen. If the control mortality rate is more than 20%, the test is redone.

The calculation method comprises the following steps:

TABLE 6 testing of a combination of a Compound of formula I and a Compound II on Phyllotreta striolata

The test results in tables 1-6 show that the combination of the compound of formula I and the compound II has synergistic effect on controlling cabbage aphids, brown planthoppers, rice leaf rollers, striped rice borers, diamond back moths and phyllotreta striolata.

Claims

1. A pesticidal composition comprising as active ingredients a compound of formula I and a compound II:

a compound of formula I

The compound II is selected from one of the following compounds: trifluoro-benzene pyrimidine, thiocyclam, hydrazine inhibitor and bromofenoxanil.

2. The insecticidal composition according to claim 1, wherein said compound II is selected from the group consisting of trifluorobenzene pyrimidines.

3. The insecticidal composition according to claim 1, wherein said compound II is selected from the group consisting of thiocyclates.

4. An insecticidal composition according to claim 1 wherein said compound II is selected from the group consisting of picrophorin.

5. An insecticidal composition according to claim 1 wherein said compound II is selected from the group consisting of bromofenoxaprop-p-fluorobenzenediamide.

6. The insecticidal composition according to claims 1 to 5, wherein the weight ratio of the compound of formula I to the compound II is 50:1 to 1:300, preferably 25:1 to 1:200, preferably 10:1 to 1:150, more preferably 10:1 to 1:100, more preferably 10:1 to 1: 50.

7. The insecticidal composition according to claim 1, further comprising a surfactant and/or a filler.

8. The insecticidal composition of claim 1 wherein the weight of said compound of formula I and compound II together comprise from 1% to 90%, preferably from 5% to 80%, more preferably from 5% to 70%, still more preferably from 5% to 60%, more preferably from 5% to 50% by weight of said insecticidal composition.

9. The insecticidal composition of claim 1, wherein the formulation of the insecticidal composition is missible oil, aqueous suspension, oil suspension, dry powder for seed treatment, suspension for seed treatment, water dispersible granule, wettable powder, suspoemulsion, aerosol, coated granule, extruded granule, aqueous emulsion, microcapsule suspension-suspension, dry suspension, ultra-low volume liquid, electrostatic oil, gel, granule, and fine granule.

10. Use of the pesticidal composition according to claim 1 for preventing or controlling pests and their larvae and eggs.

11. Use of the pesticidal composition of claim 1 for preventing or controlling pests of the families of snout moth, plutella, noctuidae, diabrotica, planthopper, aphididae, tetranychidae.

12. Use of the pesticidal composition of claim 1 for preventing or controlling aphids of tobacco, cotton, peach, diamond back moth, beet armyworm, spodoptera frugiperda, cotton bollworm, chilo suppressalis, cnaphalocrocis medinalis guenee, phyllotreta striolata, apes lineolata, apes petiolatus, tetranychus urticae, and larvae and eggs thereof.

13. A method for preventing or controlling pests and their larvae and eggs, characterized in that the pesticidal composition according to claim 1 is applied to the target useful plants, the target pests or their environment, propagation material of the target useful plants.

14. A method for preventing or controlling pests and their larvae and eggs, characterized in that the pesticidal composition of claim 1 is applied to seeds of target useful plants, target useful plants or soil where plants grow or soil suitable for plant growth.

15. A method for preventing or controlling pests and their larvae and eggs, characterized in that the pesticidal composition according to claim 1 is applied to the soil before, after or before germination of the seeds and/or directly to the soil in contact with the roots of the plants or to the soil suitable for the growth of the plants.

16. A method for protecting seeds, characterized in that the seeds are contacted with a pesticidally effective amount of the pesticidal composition according to claim 1 before sowing and/or after pregermination.