CN112566647A - Blood sugar level increase inhibitor, diabetes inhibitor and food composition - Google Patents
Blood sugar level increase inhibitor, diabetes inhibitor and food composition Download PDFInfo
- Publication number
- CN112566647A CN112566647A CN201980052741.5A CN201980052741A CN112566647A CN 112566647 A CN112566647 A CN 112566647A CN 201980052741 A CN201980052741 A CN 201980052741A CN 112566647 A CN112566647 A CN 112566647A
- Authority
- CN
- China
- Prior art keywords
- inhibitor
- carotenoid
- blood glucose
- carotenoid mixture
- glucose level
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 112
- 210000004369 blood Anatomy 0.000 title claims abstract description 75
- 239000008280 blood Substances 0.000 title claims abstract description 75
- 235000013305 food Nutrition 0.000 title claims abstract description 47
- 239000003112 inhibitor Substances 0.000 title claims abstract description 45
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 35
- 235000021466 carotenoid Nutrition 0.000 claims abstract description 109
- 150000001747 carotenoids Chemical class 0.000 claims abstract description 109
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 59
- 239000008103 glucose Substances 0.000 claims abstract description 59
- 241000195620 Euglena Species 0.000 claims abstract description 44
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 claims abstract description 40
- HNYJHQMUSVNWPV-DRCJTWAYSA-N Diatoxanthin Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1C#CC(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)C[C@@H](O)CC1(C)C HNYJHQMUSVNWPV-DRCJTWAYSA-N 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 21
- JKQXZKUSFCKOGQ-JLGXGRJMSA-N (3R,3'R)-beta,beta-carotene-3,3'-diol Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C JKQXZKUSFCKOGQ-JLGXGRJMSA-N 0.000 claims abstract description 20
- JKQXZKUSFCKOGQ-LQFQNGICSA-N Z-zeaxanthin Natural products C([C@H](O)CC=1C)C(C)(C)C=1C=CC(C)=CC=CC(C)=CC=CC=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C JKQXZKUSFCKOGQ-LQFQNGICSA-N 0.000 claims abstract description 20
- QOPRSMDTRDMBNK-RNUUUQFGSA-N Zeaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCC(O)C1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C QOPRSMDTRDMBNK-RNUUUQFGSA-N 0.000 claims abstract description 20
- JKQXZKUSFCKOGQ-LOFNIBRQSA-N all-trans-Zeaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C JKQXZKUSFCKOGQ-LOFNIBRQSA-N 0.000 claims abstract description 20
- 235000010930 zeaxanthin Nutrition 0.000 claims abstract description 20
- 239000001775 zeaxanthin Substances 0.000 claims abstract description 20
- 229940043269 zeaxanthin Drugs 0.000 claims abstract description 20
- HNYJHQMUSVNWPV-QWJHPLASSA-N Diatoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C#CC1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C HNYJHQMUSVNWPV-QWJHPLASSA-N 0.000 claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims abstract description 9
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 claims description 20
- 229960005375 lutein Drugs 0.000 claims description 20
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 claims description 20
- 230000002401 inhibitory effect Effects 0.000 claims description 17
- AQLRNQCFQNNMJA-UHFFFAOYSA-N fucoxanthin Natural products CC(=O)OC1CC(C)(C)C(=C=CC(=CC=CC(=CC=CC=C(/C)C=CC=C(/C)C(=O)CC23OC2(C)CC(O)CC3(C)C)C)CO)C(C)(O)C1 AQLRNQCFQNNMJA-UHFFFAOYSA-N 0.000 claims description 15
- SJWWTRQNNRNTPU-ABBNZJFMSA-N fucoxanthin Chemical compound C[C@@]1(O)C[C@@H](OC(=O)C)CC(C)(C)C1=C=C\C(C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C(=O)C[C@]1(C(C[C@H](O)C2)(C)C)[C@]2(C)O1 SJWWTRQNNRNTPU-ABBNZJFMSA-N 0.000 claims description 15
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims description 14
- 235000013793 astaxanthin Nutrition 0.000 claims description 14
- 239000001168 astaxanthin Substances 0.000 claims description 14
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims description 14
- 229940022405 astaxanthin Drugs 0.000 claims description 14
- 235000012680 lutein Nutrition 0.000 claims description 14
- 239000001656 lutein Substances 0.000 claims description 14
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 claims description 14
- 235000008210 xanthophylls Nutrition 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 230000037406 food intake Effects 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 235000009200 high fat diet Nutrition 0.000 description 25
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 22
- 241000699670 Mus sp. Species 0.000 description 16
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- 230000037396 body weight Effects 0.000 description 12
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 12
- 210000004185 liver Anatomy 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 10
- 125000001895 carotenoid group Chemical group 0.000 description 10
- 235000012000 cholesterol Nutrition 0.000 description 10
- 238000000605 extraction Methods 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 238000004809 thin layer chromatography Methods 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 150000002632 lipids Chemical class 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 239000012046 mixed solvent Substances 0.000 description 8
- 239000000843 powder Substances 0.000 description 8
- 241000195619 Euglena gracilis Species 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 7
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 6
- 241000195623 Euglenida Species 0.000 description 6
- 102000004877 Insulin Human genes 0.000 description 6
- 108090001061 Insulin Proteins 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 229940125396 insulin Drugs 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000004445 quantitative analysis Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 244000025670 Eleusine indica Species 0.000 description 5
- 235000013361 beverage Nutrition 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000008194 pharmaceutical composition Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 229910000365 copper sulfate Inorganic materials 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 238000010298 pulverizing process Methods 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- 235000012424 soybean oil Nutrition 0.000 description 4
- 239000003549 soybean oil Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 208000008589 Obesity Diseases 0.000 description 3
- 241000199919 Phaeophyceae Species 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 235000013402 health food Nutrition 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004811 liquid chromatography Methods 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- -1 nitrogen source Chemical class 0.000 description 3
- 235000020824 obesity Nutrition 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 229940124597 therapeutic agent Drugs 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 2
- DVICWXUADSCSLL-DDEWRDOISA-N Alloxanthin/Tetradehydrozeaxanthin/(Cynthiaxanthin)/(Pectenoxanthin) Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1C#CC(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C#CC1=C(C)C[C@@H](O)CC1(C)C DVICWXUADSCSLL-DDEWRDOISA-N 0.000 description 2
- 241000206761 Bacillariophyta Species 0.000 description 2
- 208000031648 Body Weight Changes Diseases 0.000 description 2
- 241000196240 Characeae Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000192700 Cyanobacteria Species 0.000 description 2
- OGHZCSINIMWCSB-GHIQLMQGSA-N Diadinoxanthin Chemical compound C(\[C@]12[C@@](O1)(C)C[C@@H](O)CC2(C)C)=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)C#CC1=C(C)C[C@@H](O)CC1(C)C OGHZCSINIMWCSB-GHIQLMQGSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010022489 Insulin Resistance Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 230000004579 body weight change Effects 0.000 description 2
- FDSDTBUPSURDBL-LOFNIBRQSA-N canthaxanthin Chemical compound CC=1C(=O)CCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)CCC1(C)C FDSDTBUPSURDBL-LOFNIBRQSA-N 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 210000003763 chloroplast Anatomy 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 229930186770 diadinoxanthin Natural products 0.000 description 2
- OGHZCSINIMWCSB-WEWHBREISA-N diadinoxanthin A Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C#CC1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC23OC2(C)CC(O)CC3(C)C OGHZCSINIMWCSB-WEWHBREISA-N 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 208000004104 gestational diabetes Diseases 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 201000001421 hyperglycemia Diseases 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 230000000069 prophylactic effect Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- NLAWPKPYBMEWIR-SKYQDXIQSA-N (2S)-poncirin Chemical compound C1=CC(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@H](O)[C@@H](O)[C@H](C)O3)O)=CC(O)=C2C(=O)C1 NLAWPKPYBMEWIR-SKYQDXIQSA-N 0.000 description 1
- DMASLKHVQRHNES-UPOGUZCLSA-N (3R)-beta,beta-caroten-3-ol Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C DMASLKHVQRHNES-UPOGUZCLSA-N 0.000 description 1
- ABTRFGSPYXCGMR-KXQOOQHDSA-N (3R)-beta,psi-caroten-3-ol Chemical compound CC(C)=CCCC(C)=CC=CC(C)=CC=CC(C)=CC=CC=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C ABTRFGSPYXCGMR-KXQOOQHDSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000206751 Chrysophyceae Species 0.000 description 1
- 241000196224 Codium Species 0.000 description 1
- 241001282408 Crypthecodinium sp. Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- PVNVIBOWBAPFOE-UHFFFAOYSA-N Dinoxanthin Natural products CC1(O)CC(OC(=O)C)CC(C)(C)C1=C=CC(C)=CC=CC(C)=CC=CC=C(C)C=CC=C(C)C=CC1(C(CC(O)C2)(C)C)C2(C)O1 PVNVIBOWBAPFOE-UHFFFAOYSA-N 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 241001595357 Euglena deses var. intermedia Species 0.000 description 1
- 238000013218 HFD mouse model Methods 0.000 description 1
- 206010020710 Hyperphagia Diseases 0.000 description 1
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 101000763602 Manilkara zapota Thaumatin-like protein 1 Proteins 0.000 description 1
- 101000763586 Manilkara zapota Thaumatin-like protein 1a Proteins 0.000 description 1
- 238000003820 Medium-pressure liquid chromatography Methods 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101000966653 Musa acuminata Glucan endo-1,3-beta-glucosidase Proteins 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 229910004619 Na2MoO4 Inorganic materials 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- BBJQPKLGPMQWBU-UHFFFAOYSA-N Palmitinsaeurecholesterylester Natural products C12CCC3(C)C(C(C)CCCC(C)C)CCC3C2CC=C2C1(C)CCC(OC(=O)CCCCCCCCCCCCCCC)C2 BBJQPKLGPMQWBU-UHFFFAOYSA-N 0.000 description 1
- OOUTWVMJGMVRQF-DOYZGLONSA-N Phoenicoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)C(=O)C(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)C(=O)CCC2(C)C OOUTWVMJGMVRQF-DOYZGLONSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- NLAWPKPYBMEWIR-VGQRFNKBSA-N Poncirin Natural products O([C@H]1[C@@H](O)[C@H](O)[C@H](CO)O[C@H]1Oc1cc(O)c2C(=O)C[C@@H](c3ccc(OC)cc3)Oc2c1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](C)O1 NLAWPKPYBMEWIR-VGQRFNKBSA-N 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 239000004216 Rhodoxanthin Substances 0.000 description 1
- VWXMLZQUDPCJPL-ZDHAIZATSA-N Rhodoxanthin Chemical compound CC\1=CC(=O)CC(C)(C)C/1=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C1\C(C)=CC(=O)CC1(C)C VWXMLZQUDPCJPL-ZDHAIZATSA-N 0.000 description 1
- VWXMLZQUDPCJPL-XPZLFLLQSA-N Rhodoxanthin Natural products O=C1C=C(C)/C(=C\C=C(/C=C/C=C(\C=C\C=C\C(=C/C=C/C(=C\C=C\2/C(C)=CC(=O)CC/2(C)C)/C)\C)/C)\C)/C(C)(C)C1 VWXMLZQUDPCJPL-XPZLFLLQSA-N 0.000 description 1
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- BAECOWNUKCLBPZ-HIUWNOOHSA-N Triolein Natural products O([C@H](OCC(=O)CCCCCCC/C=C\CCCCCCCC)COC(=O)CCCCCCC/C=C\CCCCCCCC)C(=O)CCCCCCC/C=C\CCCCCCCC BAECOWNUKCLBPZ-HIUWNOOHSA-N 0.000 description 1
- PHYFQTYBJUILEZ-UHFFFAOYSA-N Trioleoylglycerol Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCCCCCCCC)COC(=O)CCCCCCCC=CCCCCCCCC PHYFQTYBJUILEZ-UHFFFAOYSA-N 0.000 description 1
- 239000004213 Violaxanthin Substances 0.000 description 1
- SZCBXWMUOPQSOX-LOFNIBRQSA-N Violaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C12OC1(C)CC(O)CC2(C)C)C=CC=C(/C)C=CC34OC3(C)CC(O)CC4(C)C SZCBXWMUOPQSOX-LOFNIBRQSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- QZXMUPATKGLZAP-DXLAUQRQSA-N [(2S)-1-hexadecanoyloxy-3-[(2R,3R,4S,5R,6R)-3,4,5-trihydroxy-6-[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxypropan-2-yl] (9Z,12Z)-octadeca-9,12-dienoate Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](OC[C@@H](COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC)O[C@@H]1CO[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 QZXMUPATKGLZAP-DXLAUQRQSA-N 0.000 description 1
- 238000004847 absorption spectroscopy Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229940124358 agent for type 2 diabetes Drugs 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- DVICWXUADSCSLL-GUPSQEAKSA-N all-trans-Alloxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C#CC1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C#CC2=C(C)CC(O)CC2(C)C DVICWXUADSCSLL-GUPSQEAKSA-N 0.000 description 1
- VWXMLZQUDPCJPL-JCFHCUBBSA-N all-trans-Rhodoxanthin Natural products CC(=C/C=C/C(=C/C=C/1C(=CC(=O)CC1(C)C)C)/C)C=CC=CC(=CC=CC(=CC=C2/C(=CC(=O)CC2(C)C)C)C)C VWXMLZQUDPCJPL-JCFHCUBBSA-N 0.000 description 1
- NBZANZVJRKXVBH-ITUXNECMSA-N all-trans-alpha-cryptoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CCCC2(C)C)C NBZANZVJRKXVBH-ITUXNECMSA-N 0.000 description 1
- PGYAYSRVSAJXTE-CLONMANBSA-N all-trans-neoxanthin Chemical compound C(\[C@]12[C@@](O1)(C)C[C@@H](O)CC2(C)C)=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)C=C=C1C(C)(C)C[C@H](O)C[C@@]1(C)O PGYAYSRVSAJXTE-CLONMANBSA-N 0.000 description 1
- UFRRRMXNFIGHPC-CPZJCIGYSA-N alloxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C#CC1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC#CC2=C(C)CC(O)CC2(C)C UFRRRMXNFIGHPC-CPZJCIGYSA-N 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000003178 anti-diabetic effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 1
- 235000002360 beta-cryptoxanthin Nutrition 0.000 description 1
- DMASLKHVQRHNES-ITUXNECMSA-N beta-cryptoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)CCCC2(C)C DMASLKHVQRHNES-ITUXNECMSA-N 0.000 description 1
- 239000011774 beta-cryptoxanthin Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000012682 canthaxanthin Nutrition 0.000 description 1
- 239000001659 canthaxanthin Substances 0.000 description 1
- 229940008033 canthaxanthin Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- BBJQPKLGPMQWBU-JADYGXMDSA-N cholesteryl palmitate Chemical compound C([C@@H]12)C[C@]3(C)[C@@H]([C@H](C)CCCC(C)C)CC[C@H]3[C@@H]1CC=C1[C@]2(C)CC[C@H](OC(=O)CCCCCCCCCCCCCCC)C1 BBJQPKLGPMQWBU-JADYGXMDSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 235000016213 coffee Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 235000021557 concentrated beverage Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 235000011850 desserts Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 210000001596 intra-abdominal fat Anatomy 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 229960002725 isoflurane Drugs 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 235000020124 milk-based beverage Nutrition 0.000 description 1
- FIJGNIAJTZSERN-DQQGJSMTSA-N monogalactosyl-diacylglycerol Chemical compound CCCCCCCCCCCCCCCC(=O)O[C@H](COC(=O)CCCCCCCCCCCC)CO[C@@H]1O[C@@H](CO)[C@H](O)[C@H](O)[C@@H]1O FIJGNIAJTZSERN-DQQGJSMTSA-N 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- OWAAYLVMANNJOG-OAKWGMHJSA-N neoxanthin Natural products CC(=C/C=C(C)/C=C/C=C(C)/C=C=C1C(C)(C)CC(O)CC1(C)O)C=CC=C(/C)C=CC23OC2(C)CC(O)CC3(C)C OWAAYLVMANNJOG-OAKWGMHJSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000004305 normal phase HPLC Methods 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000020830 overeating Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 235000019246 rhodoxanthin Nutrition 0.000 description 1
- 235000009514 rubixanthin Nutrition 0.000 description 1
- 239000000455 rubixanthin Substances 0.000 description 1
- ABTRFGSPYXCGMR-SDPRXREBSA-N rubixanthin Natural products O[C@H]1CC(C)(C)C(/C=C/C(=C\C=C\C(=C/C=C/C=C(\C=C\C=C(/C=C/C=C(\CC/C=C(\C)/C)/C)\C)/C)\C)/C)=C(C)C1 ABTRFGSPYXCGMR-SDPRXREBSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 239000004250 tert-Butylhydroquinone Substances 0.000 description 1
- 235000019281 tert-butylhydroquinone Nutrition 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 description 1
- 229940117972 triolein Drugs 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000001631 vena cava inferior Anatomy 0.000 description 1
- 235000019245 violaxanthin Nutrition 0.000 description 1
- SZCBXWMUOPQSOX-PSXNNQPNSA-N violaxanthin Chemical compound C(\[C@@]12[C@](O1)(C)C[C@H](O)CC2(C)C)=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)/C=C/[C@]1(C(C[C@@H](O)C2)(C)C)[C@]2(C)O1 SZCBXWMUOPQSOX-PSXNNQPNSA-N 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
- 150000003735 xanthophylls Chemical class 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/68—Protozoa, e.g. flagella, amoebas, sporozoans, plasmodium or toxoplasma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Obesity (AREA)
- Endocrinology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Emergency Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a blood sugar rise inhibitor, a diabetes inhibitor and a food composition, which are new using methods of carotenoid mixtures derived from microalgae. The present invention relates to a blood glucose level increase inhibitor, a diabetes inhibitor, and a food composition containing a carotenoid mixture derived from microalgae as an active ingredient, the carotenoid mixture containing diatoxanthin as a main component. For example, the carotenoid mixture derived from microalgae is a carotenoid mixture derived from euglena, contains diatoxanthin as a main component, and contains zeaxanthin and isoflavin. In addition, for example, it can be used to suppress an increase in blood glucose level by taking it together with a high GI food.
Description
Technical Field
The present invention relates to a novel blood glucose level increase inhibitor, a novel diabetes inhibitor and a novel food composition, and particularly relates to a blood glucose level increase inhibitor, a novel diabetes inhibitor and a novel food composition each containing a carotenoid mixture derived from microalgae as an active ingredient.
Background
In general, the glucose concentration in blood is regulated within a certain range in a living body by the action of insulin, and diabetes is a disease in which the glucose regulation function does not work normally and the glucose concentration in blood rises abnormally.
Diabetes mellitus is classified roughly into type 1 diabetes and type 2 diabetes, and type 1 diabetes is a disease in which β cells in the pancreas are destroyed and insulin cannot be secreted from the pancreas.
More specifically, it is said that this is a disease in which abnormal insulin secretion and insulin resistance are caused due to abnormal accumulation of triglycerides in blood and organs.
Under such circumstances, many therapeutic agents have been developed for type 2 diabetes, which is said to account for 90% or more of diabetes patients, particularly japanese diabetes patients, but there are many cases where sufficient clinical results, mental symptoms, side effects on the heart, and the like are not obtained.
Therefore, there is a need for the development of a blood glucose level increase inhibitor and a diabetes inhibitor containing a natural substance as an active ingredient, which are safe and effective.
On the other hand, carotenoids are useful as natural pigments and have various effective actions such as antioxidant action, and therefore, the utility value in the fields of pharmaceuticals, foods, cosmetics, and the like is increasing.
As a diabetes inhibitor using carotenoids, for example, patent document 1 discloses a carotenoid mixture containing fucoxanthin as a main component. Specifically, it is disclosed that fucoxanthin is an extract derived from microalgae, shows anti-diabetic properties, and that fucoxanthin is desirable in combination with other carotenoids having nutritional auxiliary value.
Further, non-patent document 1 discloses an effect of preventing lifestyle-related diseases by ingesting astaxanthin derived from microalgae. Specifically, it has been reported that astaxanthin is administered to mice fed a high-fat diet, and the visceral fat mass, the blood glucose level in the fasting state, and the insulin value of the mice are improved.
Among the carotenoids, a typical carotenoid other than fucoxanthin and astaxanthin is diatom xanthin contained in microalgae such as phytoplankton, brown algae, and diatoms (Diathoxanthin, CAS registry No. 31063-73-7).
Diatomxanthin is one of xanthophylls, and is a carotenoid having a unique structure in which a triple bond is present in a polymer.
Diatomxanthin is expected to have an antioxidant effect as well as other carotenoids, but its functionality is not well understood because it is difficult to obtain in large quantities and is expensive.
Therefore, it is necessary to elucidate the mechanism of the function and the expression of the function of a carotenoid mixture containing, as a main component, diatom xanthin which is a carotenoid other than fucoxanthin and astaxanthin, and to develop a method for utilizing these substances.
Documents of the prior art
Patent document
Patent document 1: japanese Kohyo publication (Kohyo publication) No. 2018-512432
Non-patent document 1: YInhua Ni et al, "Astaxannthin precursors and recoveres di et-induced insulin resistance and stephanism in mic: a, composition with vitamin E ", Scientific Reports, 5: 17192(2015)
Disclosure of Invention
Problems to be solved by the invention
The present invention has been made in view of the above problems, and an object of the present invention is to provide a novel blood glucose level increase inhibitor, a diabetes inhibitor, and a food composition.
Another object of the present invention is to provide a blood glucose level increase inhibitor, a diabetes inhibitor, and a food composition, which are novel methods for using a carotenoid mixture derived from microalgae containing diatoxanthin as a main component.
Means for solving the problems
The inventors of the present invention have conducted extensive studies and found, as a result, that: a carotenoid mixture derived from microalgae containing xanthophyll, especially diatomaceous xanthine as main component has effect in inhibiting blood glucose level increase.
Therefore, according to the present invention, the above problems are solved by an inhibitor for the increase in blood glucose level, which comprises a carotenoid mixture derived from microalgae as an active ingredient, wherein the carotenoid mixture contains lutein (except fucoxanthin and astaxanthin) as a main component.
In this case, the xanthophyll may be xanthophyll, and the microalgae may be euglena.
The carotenoid mixture may contain diatoxanthin as a main component, and may contain one or more compounds selected from zeaxanthin and isoxanthin, and may further contain 5.0% to 80.0% of diatoxanthin.
In addition, the carotenoid mixture may comprise 10.0-80.0% of diatoxanthin, 2.0-20.0% of zeaxanthin and 0.1-6.0% of isoflavin.
Further, it can be used for inhibiting the increase in blood glucose level after ingestion of a high GI food by ingestion together with the high GI food. Further, it can be used for inhibiting the increase of blood glucose level by oral ingestion for at least 6 consecutive weeks.
As described above, by ingesting the carotenoid mixture of the present invention together with a high GI food, it is possible to effectively suppress an increase in blood glucose level when ingesting a high GI food.
Herein, "food with high GI" refers to food with high GI (Glycemic Index), and "GI" is an Index of the rate of increase in blood glucose level after intake of carbohydrates.
As GI increases, the blood glucose level rises rapidly after food intake, and when the blood glucose level rises rapidly, the pancreas secretes a large amount of insulin rapidly. The insulin converts blood glucose into energy, and on the other hand, excess sugar is stored in adipose tissue. Therefore, from the viewpoint of lifestyle-related diseases including diabetes, high GI foods should be avoided as much as possible.
In addition, the above problems are also solved by the following diabetes inhibitors: contains carotenoid mixture derived from microalgae as effective component, wherein the carotenoid mixture contains xanthophyll (except fucoxanthin and astaxanthin) as main component.
Further, the above problems are also solved by the following food compositions for inhibiting an increase in blood glucose level or for inhibiting diabetes: contains carotenoid mixture derived from microalgae as effective component, wherein the carotenoid mixture contains xanthophyll (except fucoxanthin and astaxanthin) as main component.
Further, the above problem is also solved by the following manufacturing method:
the method is used for producing a carotenoid mixture containing lutein (except fucoxanthin and astaxanthin) as a main component for inhibiting blood sugar level increase or diabetes, and comprises:
a culture step of culturing microalgae having the ability to produce the carotenoid mixture, and
and a step of obtaining the carotenoid mixture from the microalgae obtained in the culture step.
ADVANTAGEOUS EFFECTS OF INVENTION
The present invention can provide a novel blood glucose level increase inhibitor, a novel diabetes inhibitor, and a novel food composition.
Further, it is possible to provide an inhibitor of increase in blood glucose level, an inhibitor of diabetes, and a food composition which are novel methods of using a carotenoid mixture derived from microalgae containing diatoxanthin as a main component.
Drawings
FIG. 1 is a chromatogram of HPLC quantitative analysis of a carotenoid mixture derived from Euglena (item 1).
FIG. 2 is a chromatogram of HPLC quantitative analysis of a carotenoid mixture derived from Euglena (2 of).
FIG. 3 shows the results of TLC analysis of neutral lipids in carotenoid mixtures.
FIG. 4 shows the results of TLC analysis of polar lipids in carotenoid mixtures.
FIG. 5 is a graph comparing the change in body weight of mice given the respective groups of feeds for 6 weeks.
FIG. 6 is a graph comparing the change in liver weight after 6 weeks of administration of the respective groups of feeds to mice.
Fig. 7 is a graph comparing the values of total cholesterol in blood 6 weeks after the mice were given the respective groups of feeds.
Fig. 8 is a graph comparing the glucose values in blood 6 weeks after administration of each group of feeds to mice.
Detailed Description
An embodiment of the present invention will be described with reference to fig. 1 to 8.
The present embodiment relates to an inhibitor of blood glucose level increase, an inhibitor of diabetes, and a food composition, which contain, as an active ingredient, a carotenoid mixture derived from microalgae containing lutein (excluding fucoxanthin and astaxanthin) as a main ingredient.
The present invention also relates to a method for producing an agent for inhibiting an increase in blood glucose level, the agent containing, as an active ingredient, a carotenoid mixture containing lutein as a main component, the method comprising: a culture process of culturing microalgae having an ability to produce a carotenoid mixture, and
a carotenoid mixture obtaining step of obtaining a carotenoid mixture from the obtained microalgae.
< Carotenoid mixture derived from microalgae >
"microalgae" are plants of single cells or multicellular bodies of about several μm to several tens of μm in size, which live mainly by photosynthesis in water.
Examples of microalgae include blue-green algae, euglena (euglena), stonewort, codium, xanthomonas, brown algae, red algae, diatom, stonewort, dinoflagellate, euglena, chrysophyceae, blue-green algae, or codococcus.
In this embodiment, euglena (euglena) is desirably used, but is not particularly limited.
"Euglena" includes all microorganisms classified in the zoology, phytology classification as Euglena (Euglena), variants thereof, mutants thereof.
Here, the microorganism of euglena (eugulen) means: in zoology, microorganisms belonging to the order Euglenoidina (Euglenoidina) of the order Euglenida (Euglenida), which belongs to the class of the flagellates (Mastigophorea), the subclasses of the plant Trichididae (Phytomastigophora) of the phylum Protozoa (Protozoa). On the other hand, in botany, it belongs to the order Euglena (Eaglenales) belonging to the Euglenophyceae (Euglenophyceae) of the Euglenophyta phylum (Euglenophyta).
As the euglena microorganism (euglena cell), euglena gracilis (e.gracilis), in particular, euglena gracilis (e.gracilis) Z strain, euglena gracilis (e.gracilis) NIES-49 strain, and the like can be used. Also, β -1, 3-glucanase of a gene mutant strain such as a mutant strain SM-ZK strain (chloroplast deletion strain) of Euglena gracilis (e.gracilis) Z strain, a mutant strain of a mutant of a chloroplast derived from these species, or Euglena intermedia, Euglena piride, or other Euglena species may be used.
Euglena is widely distributed in fresh water such as ponds and marshes, and can be used by being separated from these, or any euglena that has been separated can be used.
In the present embodiment, the euglena gracilis is preferably used, and the euglena gracilis (e.gracilis) strain Z or NIES-49 is particularly preferably used, but is not particularly limited.
The "cultivation of euglena cells" can be performed using a culture solution. Adding carbon source including inorganic carbon source (CO) as nutrient source into the culture solution2,NaHCO3,Na2CO3Etc.) and organic carbon sources (glucose, etc.). As the culture solution used in the present embodiment, an independent nutrient medium containing no organic carbon source such as glucose as a nutrient source is preferably used, but the present invention is not limited thereto. For example, a culture solution supplemented with nutrient salts such as nitrogen source, phosphorus source, minerals, etc., such as Cramer-Myers medium, modified Cramer-Myers medium ((NH)4)2HPO4 1.0g/L、KH2PO4 1.0g/L、MgSO4·7H2O 0.2g/l、CaCl2·2H2O 0.02g/l、Fe2(SO2)3·7H2O 3mg/l、MnCl2·4H2O 1.8mg/l、CoSO4·7H2O 1.5mg/l、ZnSO4·7H2O 0.4mg/l、Na2MoO4·2H2O 0.2mg/l、CuSO4·5H2O0.02 g/l, thiamine hydrochloride (vitamin B1)0.1mg/l, cyanocobalamin (vitamin B12) (pH 3.5)). To describe (NH)4)2HPO4May also be converted into (NH)4)2SO4Or NH3An aqueous solution.
The pH of the culture medium is preferably 2 or more, and the upper limit thereof is preferably 6 or less, more preferably 4.5 or less. By setting the pH to the acidic side, the photosynthetic microorganisms can grow more dominantly than other microorganisms, and thus contamination can be suppressed.
The cultivation of euglena cells can also be carried out in the following manner: an open cell system directly utilizing sunlight, and a light collecting system in which sunlight collected by a light collecting device is transmitted through an optical fiber or the like, irradiated into a culture tank, and utilized by photosynthesis.
The euglena cells may be cultured by, for example, a batch feeding method, but may be cultured by: flask culture, culture using a fermenter, batch culture, semibatch culture (fed-batch culture), continuous culture (perfusion culture), any liquid culture method.
The separation of euglena cells is performed, for example, by centrifugation or simple sedimentation of a culture solution.
The "carotenoid mixture" is a mixture containing one kind of lutein as a main component and, in addition, containing one or more compounds selected from other compounds in the lutein as an accessory component.
"lutein" is a generic term for pigments in carotenoids containing oxygen in the form of hydroxyl, carbonyl or epoxy groups, specifically lutein contains: epoxidized zeaxanthin, astaxanthin, canthaxanthin, poncirin, beta-cryptoxanthin, diadinoxanthin, diatoxanthin, diadinoxanthin, ranuncuxanthin, fucoxanthin, lutein, neoxanthin, rhodoxanthin, rubixanthin, violaxanthin, and zeaxanthin.
Specifically, the carotenoid mixture of the present embodiment is a mixture containing diatoxanthin as a main component and one or more compounds selected from zeaxanthin and isoflavin as an auxiliary component, and more preferably a mixture containing both zeaxanthin and isoflavin as an auxiliary component.
It is noted that the carotenoid mixture may further comprise carotenoids (lutein) other than these.
"Diatomxanthin" is a carotenoid having a structure with a triple bond in a polymer, as shown in the following formula (chemical formula 1) (Diathoxanthin, CAS registry No. 31063-73-7, chemical formula C)40H54O2Molecular weight: 566.87).
The diatomaceous xanthins are contained in microalgae such as phytoplankton, brown algae, and diatoms, and are obtained from euglena in the present embodiment.
[ chemical formula 1]
"Zeaxanthin" is a carotenoid having a structure represented by the following formula (chemical formula 2) (Zeaxanthin, CAS registry number: 144-68-3, chemical formula C)40H54O2Molecular weight: 568.89), it is a component that is contained in edible vegetables, bacteria, algae such as spirulina, and is ingested in large quantities in daily dietary life.
Zeaxanthin can be used for coloring food, resisting obesity and cancer, and its derivative has antioxidant effect.
[ chemical formula 2]
"Isoflavin" is a carotenoid having a structure with two triple bonds in a polymer (Alloxanthin, CAS registry No.: 28380-31-6, formula C) as shown in the following formula (formula 3)40H52O2Molecular weight: 564.85) contained in Crypthecodinium sp.
[ chemical formula 3]
The carotenoid mixture of the present embodiment may contain, in terms of a ratio of peak areas in a chromatogram for quantitative analysis by liquid chromatography using a detector having a detection wavelength of 450 nm: 5.0-80.0% of diatoxanthine, 1.0-20.0% of zeaxanthin and 0.1-10.0% of isoflavin relative to 100% of the total weight of the carotenoid mixture.
In addition, the composition preferably comprises 10.0-80.0% of diatomite, 2.0-20.0% of zeaxanthin and 0.1-6.0% of isoflavin.
< method for producing Carotenoid mixture >
The carotenoid mixture of the present embodiment is produced by a production method which performs the following steps: the "culturing step" of culturing euglena under the above culturing conditions, the "separating step" of separating euglena, the "pulverizing step" of pulverizing the separated euglena, the "dispersing step" of dispersing the pulverized euglena in an extraction solvent, the "extracting step" of extracting a carotenoid extract from the dispersed euglena, and the "obtaining step" of obtaining a carotenoid fraction (carotenoid mixture) containing diatoxanthin from the carotenoid extract.
First, in the culture step (step S1), euglena is cultured under the above culture conditions. Specifically, euglena was cultured under independent culture conditions.
Next, in the separation step (step S2), euglena cultured in the above-described culture step is separated. Specifically, the separation of euglena is performed by a known separation method such as centrifugation or simple sedimentation of a culture solution, membrane filtration, or the like, but is not particularly limited.
The separated euglena is washed and then dried by a known drying method (vacuum freeze drying, spray drying, heat vacuum drying, etc.), thereby preparing a dry euglena algal body.
Next, in the pulverization step (step S3), the euglena separated in the separation step is pulverized by a known pulverization method. Specifically, there may be mentioned a method of adding an extraction solvent to the separated euglena and crushing the euglena by an ultrasonic crusher or the like, but the method is not particularly limited.
Next, in the dispersion step (step S4), the euglena cells crushed in the crushing step are dispersed in the extraction solvent to obtain a dispersion liquid.
Specifically, as the extraction solvent used in the dispersion step, a lipophilic polar solvent such as acetonitrile can be used.
Next, in the extraction step (step S5), a carotenoid extract is extracted from the euglena dispersed in the extraction solvent in the dispersion step.
The extraction conditions are not particularly limited, and the extraction of carotenoids can be promoted by heating, applying external stimulation such as ultrasonic waves, and shaking.
Finally, in the obtaining step (step S6), a carotenoid fraction (carotenoid mixture) containing diatoxanthin is obtained from the carotenoid extract obtained in the above-described extraction step.
Specific examples of the fractionation method include a method of obtaining a fraction having a high content of diatomaceous earths by fractionation using an appropriate separation means (for example, reverse-phase or normal-phase high performance liquid chromatography partition extraction, gel filtration, silica gel chromatography, or the like). Alternatively, there is a method comprising filtering a carotenoid extract containing diatoxanthin, separating into an extract and a residue, concentrating the extract, and fractionating by liquid chromatography.
< uses, methods and amounts >
The carotenoid mixture of the present embodiment can be used as a therapeutic agent for diabetes (particularly type 2 diabetes) by administering to a diabetic patient or an animal other than a human having diabetes.
In addition, the compounds can also be used as a prophylactic agent for diabetes (particularly, a prophylactic agent for type 2 diabetes), and a diabetes inhibitor (particularly, a type 2 diabetes inhibitor) for a person before onset of diabetes, a person who is a potential diabetic patient, a person with hyperglycemia in a fasting state, a person with hyperglycemia after meal, and other animals except for these persons.
In addition, it can also be used as a preventive agent for gestational diabetes or an inhibitor for gestational diabetes for a pregnant person.
In addition, it is also useful as a preventive agent for complications accompanying diabetes, a complication inhibitor, or a complication therapeutic agent.
The carotenoid mixture of the present embodiment can be used as a blood glucose level increase inhibitor.
Preferably, it can be used as a blood glucose level increase inhibitor for inhibiting an increase in blood glucose level by being taken together with a food (particularly a GI food).
Further, it is preferable that the oral ingestion is performed continuously for a certain period of time, and the composition can be used as a blood glucose level increase inhibitor for inhibiting the increase in blood glucose level.
The carotenoid mixture of the present embodiment can be used as a composition such as a pharmaceutical composition or a food composition for inhibiting an increase in blood glucose level or diabetes.
In the pharmaceutical field, a pharmaceutical composition having the above-mentioned effects is provided by compounding a carotenoid mixture in an amount effective to exert the above-mentioned effects with a pharmaceutically acceptable carrier or additive. The pharmaceutical composition can be a medicine or a quasi-medicine.
The pharmaceutical composition can be administered internally or externally, and can be used in the form of preparations such as injections for oral administration, intravenous injection, subcutaneous injection, intradermal injection, intramuscular injection and/or intraperitoneal injection, transmucosal administration, transdermal administration, etc.
The dosage form of the pharmaceutical composition includes solid preparations such as tablets, granules, capsules, powders and powders, and semi-solid preparations such as liquids, ointments and gels.
In the field of foods, a carotenoid mixture in an amount effective for inhibiting an increase in blood glucose level or diabetes in a living body can be blended as a food material into various foods to provide a food composition having the above-mentioned effects.
That is, the present invention can provide a food composition which is labeled as having an effect of inhibiting an increase in blood glucose level in the field of foods. The food composition includes general foods, specific health foods, nutritional functional foods, hospital patient foods, supplements, and the like. In addition, it can be used as food additive.
Examples of the food composition include seasonings, processed livestock meat products, processed agricultural products, beverages (e.g., refreshing beverages, alcoholic beverages, carbonated beverages, milk beverages, fruit juice beverages, tea, coffee, and nutritional beverages), powdered beverages (e.g., fruit juice powder and soup powder), concentrated beverages, desserts (e.g., candies (throat drops), cookies, biscuits, chewing gums, soft sweets, and chocolates), bread, and grains. In the case of a specific health food, a food having a nutritional function, a food having a marked function, or the like, the food may be in the form of a capsule, a tablet, a syrup, a granule, a powder, or the like.
The "specific health food" is a food containing a health functional component affecting physiological functions and the like, and can be indicated as being suitable for a specific health use by approval of the consumer's office officer.
"nutrient functional food" is a food for supplementing nutrient components (vitamins, minerals), and indicates the function of the nutrient components.
The "food for indicating functionality" is a food for which the operator is responsible for indicating functionality according to scientific grounds. Before sale, the consumer's halls are notified of information regarding security and functional grounds, etc.
The amount of the blood glucose level increase inhibitor and the diabetes inhibitor of the present embodiment may be, for example, orally taken together with food (particularly GI food) to a predetermined intake amount for human or non-human animals.
Examples
< example 1>
A carotenoid mixture derived from euglena was prepared by the following sequence.
1) 20L of acetonitrile was added to 20kg of Euglena gracilis powder (manufactured by Euglena, Inc.) and disrupted by an ultrasonic disrupter to obtain a disrupted extract mixture.
2) Filtering the pulverized extract mixture, and separating into extractive solution and residue.
3) The extract was concentrated using a rotary evaporator and fractionated by medium pressure liquid chromatography. Fractionation was carried out using a liquid chromatography apparatus using an ODS column (InertSustain C18, manufactured by GL Science Co., Ltd.) as a column and acetonitrile as an eluent at a detection wavelength of 270 nm.
4) Using HPLC (high performance liquid chromatography), the carotenoid fraction containing diatoxanthin was recovered from each of the fractionated fractions and concentrated by an evaporator.
By the above method, 0.4g of a reddish brown powder was obtained, which is a carotenoid fraction containing diatoxanthin. The powder is used as a carotenoid mixture.
< test 1: HPLC quantitative analysis of Carotenoid mixtures >
Quantitative analysis of the carotenoid mixture of example 1 was performed using HPLC under the following conditions.
0.1g of the carotenoid mixture was dissolved in acetonitrile, and the resultant was filtered to obtain a substance as a measurement sample solution.
A high performance liquid chromatography apparatus was used, an LC-6AD (manufactured by Shimadzu corporation) was used as a pump, a PDA detector (SPD-M20A) was used as a detector, and an ODS column (TSKgel ODS-80Ts, manufactured by Tosoh corporation) was used as a column.
As the mobile phase, a mixed solvent of acetonitrile, methanol and water (a mixed solvent obtained by mixing at a volume ratio of 75: 15: 10) was used as the a liquid, and a mixed solvent of ethyl acetate and methanol (a mixed solvent obtained by mixing at a volume ratio of 70: 30) was used as the B liquid.
The measurement was carried out at a column temperature of 40 ℃ and a flow rate of 1.0ml/min at a detection wavelength of 450 nm.
As a result of the test in test 1, the chromatogram of example 1 is shown in fig. 1 and 2 (n is 2).
By the retention time in the ultraviolet-visible absorption spectrometry, the mass spectrometry, and the chromatography, it is estimated that zeaxanthin and isoxanthin are present in addition to diatoxanthine.
In the above quantitative analysis, the ratio (%) of each peak area in the chromatogram corresponding to diatoxanthine, zeaxanthin and isoxanthin was determined.
The ratio (%) of each peak area was determined relative to 100% of the total peak area of all the detected components (all the components are assumed to be carotenoids).
The chromatogram in FIG. 1 (example 1-1) is as follows.
Peak number 1: isoflavin 0.4%
Peak number 2: 13.3 percent of diatomite
Peak number 3: 2.1 percent of zeaxanthin
The chromatogram in FIG. 2 (example 1-2) is as follows.
Peak number 1: 5.6 percent of isoflavin
Peak number 2: 75.9 percent of diatomite
Peak number 3: zeaxanthin 18.4%
In the following experiments, the carotenoid mixture of example 1-1 was used.
< test 2: TLC analysis of Carotenoid mixtures (neutral lipids) >
TLC (thin layer chromatography) analysis of neutral lipids in the carotenoid mixture of example 1 was performed according to the following procedure.
The carotenoid mixture (0.01mg/ml, 0.1mg/ml, 1mg/ml) and standards (oleic acid, triolein, cholesteryl palmitate, cholesterol, diatrizine, zeaxanthin) were spotted onto silica gel plates, respectively.
Development was carried out using a mixed solvent of hexane, diethyl ether and acetic acid (mixed solvent obtained by mixing at a volume ratio of 65: 35: 1).
And (3) after taking a picture by using a digital camera, immersing the silica gel plate into a copper sulfate developing solution, and heating for 5-10 minutes at 180 ℃ by using a hot plate to develop color.
The mass of the carotenoid mixture was determined as the mass (mg/ml) of each 1L of the liquid medium of each of the diatrizine samples by comparing the amount with a known amount of the diatrizine standard.
The results of TLC analysis of run 2 are shown in FIG. 3. From the results of TLC analysis before and after the development of the color of copper sulfate in each sample, it was found that no neutral lipid was mixed in the carotenoid mixture.
< test 3: TLC analysis (polar lipids) of Carotenoid mixtures
TLC (thin layer chromatography) analysis of polar lipids in the carotenoid mixture of example 1 was performed by the following procedure.
Carotenoid mixtures (0.01mg/ml) and standards (phosphatidylcholine, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, diatoxanthin, zeaxanthin) were spotted onto silica gel plates, respectively.
Development was carried out using a mixed solvent of chloroform, methanol and water (mixed solvent obtained by mixing at a volume ratio of 64: 16: 2).
And (3) after taking a picture by using a digital camera, immersing the silica gel plate into a copper sulfate developing solution, and heating for 5-10 minutes at 180 ℃ by using a hot plate to develop color.
The results of TLC analysis of run 3 are shown in FIG. 4. From the results of TLC analysis before and after the development of color of copper sulfate in each sample, it was found that the carotenoid mixture was not mixed with polar lipids.
The chromatogram of example 1 is shown in FIG. 1.
< test 4: effect of Carotenoid mixtures Using high fat diet mice >
When a carotenoid mixture having a feed composition shown in table 1 below was orally taken by a mouse, "body weight change", "liver weight", "total cholesterol amount in blood" and "glucose amount in blood" were measured and tested.
In this experiment, 18C 57BL/6J mice (6-week-old males, 20g to 23g in body weight) were used (n-6). The breeding conditions were set at room temperature of 24. + -. 2 ℃ and a light-dark period of 12 hours.
First, mice were allowed to freely ingest solid feed and water and were acclimatized to the breeding environment by 10-day preliminary breeding. After the preliminary feeding was completed, three groups of 1) AIN93G basic diet groups (hereinafter referred to as "control group"), 2) high fat diet groups containing 30% by weight of lipid in the basic diet, and 3) high fat diet + carotenoid groups containing 0.04% by weight of a carotenoid mixture were given to different feeds for each group, and the test feeding was performed for 6 weeks of free intake, according to the feed composition shown in table 1 below.
[ Table 1]
The value of totals is 1000.00 by rounding off.
The carotenoid mixture in the feed was dissolved in soybean oil in the following order and mixed.
The carotenoid mixture was placed in an eggplant-shaped flask together with ethanol and dissolved. Dissolving tert-butylhydroquinone in refined soybean oil heated to 70 deg.C, returning to normal temperature, and mixing with carotenoid mixture dissolved in ethanol. The ethanol was distilled off using a rotary evaporator, and the carotenoid adhered to the eggplant-shaped flask was redissolved with ethanol, and the procedure was repeated until the carotenoid was completely mixed with the refined soybean oil. Further, refined soybean oil was bubbled with nitrogen gas to completely remove ethanol, and the feed was mixed with the carotenoid mixture.
Daily body weights were measured during the feeding period. After the 6-week test period was terminated, overnight fasted mice were laparotomized under isoflurane anesthesia, livers were harvested and blood was collected from the inferior vena cava.
After the collected liver was washed with physiological saline, the liver weight was measured.
Furthermore, the collected blood was centrifuged (2000rpm, 15 minutes, 4 degrees), and the obtained blood was collected and stored at 80 degrees. Then, the total cholesterol value and the glucose value in blood were quantified using a quantification kit (manufactured by Wako pure chemical industries, Ltd.).
As the test results, "body weight change", "liver weight", "total cholesterol in blood value" and "glucose in blood value" of the control group, the high fat diet group and the high fat diet + carotenoid group were compared, respectively, and the obtained graphs are shown in fig. 5, 6, 7, 8. In addition, respective numerical values are shown in table 2 below.
The unit "mg/dL" means the total cholesterol value (mg) and glucose value (mg) per 1dL of blood.
[ Table 2]
Regarding the body weight during the feeding period, it was found that the body weight of the mice in the "high fat diet group" and the "high fat diet + carotenoid group" tended to increase almost all the time as compared with the "control group". In addition, a tendency was found that the body weight of the "high fat diet + carotenoid group" was lower than that of the "high fat diet group".
In addition, regarding the body weight after 6 weeks of the feeding period, it was confirmed that the body weight of the mice in the "high fat diet group" and the "high fat diet + carotenoid group" was significantly increased (P < 0.05) compared to the "control group". In addition, a tendency was found that the body weight of the "high fat diet + carotenoid group" was lower than that of the "high fat diet group".
Regarding the liver weight after 6 weeks of the feeding period, a tendency was found that the liver weight of the mice of the "high fat diet group" was significantly increased compared to the "control group". On the other hand, it was found that the liver weight of the "high fat diet + carotenoid group" was not increased as compared with the "control group" but was similarly inclined.
Regarding the total cholesterol value in blood after 6 weeks of the feeding period, it was found that the total cholesterol value in blood of the mice of the "high fat diet group" and the "high fat diet + carotenoid group" was increased more than that of the "control group". In addition, a tendency was found that the total cholesterol value in the blood was reduced in the "high fat diet + carotenoid group" as compared with the "high fat diet group".
Regarding the blood glucose values after 6 weeks of the feeding period, it was found that the blood glucose values of the mice of the "high fat diet group" were significantly increased (P < 0.05) compared to the "control group". On the other hand, the blood glucose level of the "high fat diet + carotenoid group" was considered to be equivalent to that of the "control group" without increasing (P < 0.05).
From the above, the results of the tests on the change in body weight during the feeding period, the body weight after 6 weeks of the feeding period, the weight of liver and the total cholesterol value in blood show that: by orally ingesting a high-fat diet (e.g., high GI food) and the carotenoid mixture of example 1 simultaneously, the mice exhibited the effect of suppressing the increase in body weight (particularly body fat), liver weight (particularly liver fat), and blood cholesterol level after ingesting a high-fat diet.
In addition, from the results of the test of the blood glucose value after 6 weeks of the feeding period, it was found that: by orally ingesting the high-fat diet together with the carotenoid mixture of example 1, the mice exhibited an effect of suppressing the increase in blood sugar level after the intake of the high-fat diet.
The "blood glucose level" is a glucose concentration in blood, and is a value indicating how much mg of glucose is contained in 1dl blood, and corresponds to the blood glucose level in this test.
Claims (11)
1. A blood sugar level increase inhibitor contains a carotenoid mixture derived from microalgae as an active ingredient, wherein the carotenoid mixture contains lutein as a main component, and fucoxanthin and astaxanthin are excluded.
2. The inhibitor for increasing blood glucose level according to claim 1, wherein the xanthophyll is diatoxanthin.
3. The inhibitor for increasing blood glucose level according to claim 1 or 2, wherein the microalgae is euglena.
4. The inhibitor for increasing blood glucose level according to any one of claims 1 to 3, wherein the carotenoid mixture contains diatoxanthin as a main component and contains one or more compounds selected from zeaxanthin and isoxanthin.
5. The inhibitor of increase in blood glucose level according to any one of claims 1 to 4, wherein the carotenoid mixture contains 5.0 to 80.0% of diatoxanthin.
6. The inhibitor of increase in blood glucose level according to any one of claims 1 to 5, wherein the carotenoid mixture contains 10.0 to 80.0% of diatoxanthin, 2.0 to 20.0% of zeaxanthin, and 0.1 to 6.0% of isoflavin.
7. The inhibitor for increasing blood glucose level according to any one of claims 1 to 6, which is used for inhibiting an increase in blood glucose level when taken together with a high GI food.
8. The inhibitor for increasing blood glucose level according to any one of claims 1 to 7, which is used for inhibiting an increase in blood glucose level by oral ingestion thereof for at least 6 consecutive weeks.
9. A diabetes inhibitor contains, as an active ingredient, a carotenoid mixture derived from microalgae, the carotenoid mixture containing lutein as a main component, excluding fucoxanthin and astaxanthin.
10. A food composition for inhibiting blood sugar level increase or for inhibiting diabetes contains, as an active ingredient, a carotenoid mixture derived from microalgae containing lutein as a main component, excluding fucoxanthin and astaxanthin.
11. A method for producing a carotenoid mixture for inhibiting an increase in blood glucose level or diabetes, the carotenoid mixture containing lutein as a main component and excluding fucoxanthin and astaxanthin,
the method comprises the following steps:
a culture step of culturing microalgae having the ability to produce the carotenoid mixture, and
a step of obtaining the carotenoid mixture from the microalgae obtained in the culture step.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2018163672A JP7242219B2 (en) | 2018-08-31 | 2018-08-31 | Blood sugar elevation inhibitor, diabetes inhibitor, and food composition |
JP2018-163672 | 2018-08-31 | ||
PCT/JP2019/034199 WO2020045647A1 (en) | 2018-08-31 | 2019-08-30 | Agent for suppressing increment of blood glucose level, diabetes preventing agent, and food composition |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112566647A true CN112566647A (en) | 2021-03-26 |
Family
ID=69643867
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201980052741.5A Pending CN112566647A (en) | 2018-08-31 | 2019-08-30 | Blood sugar level increase inhibitor, diabetes inhibitor and food composition |
Country Status (3)
Country | Link |
---|---|
JP (1) | JP7242219B2 (en) |
CN (1) | CN112566647A (en) |
WO (1) | WO2020045647A1 (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101188995A (en) * | 2005-01-21 | 2008-05-28 | 普罗马公司 | Sunscreen compositions comprising carotenoids |
CN102935116A (en) * | 2012-12-03 | 2013-02-20 | 北京润康普瑞生物技术有限公司 | Pharmaceutical compositions or health care product having blood sugar reduction effect |
TW201517902A (en) * | 2013-11-13 | 2015-05-16 | Richin Life Code Biotechnology Co Ltd | Method of filamentous brown algae for producing fucoxanthin |
KR101848563B1 (en) * | 2016-10-06 | 2018-05-28 | 경희대학교 산학협력단 | The manufacturing methode of the dextrin particle by the muti- hydrolysis and dextrin particle prepared thereby |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009062337A (en) | 2007-09-07 | 2009-03-26 | Euglena Co Ltd | alpha-GLUCOSIDASE ACTIVITY INHIBITOR AND FOOD CONTAINING THE SAME |
-
2018
- 2018-08-31 JP JP2018163672A patent/JP7242219B2/en active Active
-
2019
- 2019-08-30 CN CN201980052741.5A patent/CN112566647A/en active Pending
- 2019-08-30 WO PCT/JP2019/034199 patent/WO2020045647A1/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101188995A (en) * | 2005-01-21 | 2008-05-28 | 普罗马公司 | Sunscreen compositions comprising carotenoids |
CN102935116A (en) * | 2012-12-03 | 2013-02-20 | 北京润康普瑞生物技术有限公司 | Pharmaceutical compositions or health care product having blood sugar reduction effect |
TW201517902A (en) * | 2013-11-13 | 2015-05-16 | Richin Life Code Biotechnology Co Ltd | Method of filamentous brown algae for producing fucoxanthin |
KR101848563B1 (en) * | 2016-10-06 | 2018-05-28 | 경희대학교 산학협력단 | The manufacturing methode of the dextrin particle by the muti- hydrolysis and dextrin particle prepared thereby |
Non-Patent Citations (3)
Title |
---|
J. DELI ET AL.,: "Carotenoid composition of three bloom-forming algae species", FOOD RESEARCH INTERNATIONAL, vol. 65, pages 215 - 223 * |
RYOKO SHIMADA ET AL.,: "Oral administration of green algae, Euglena gracilis, inhibits hyperglycemia in OLETF rats, a model of spontaneous type 2 diabetes", THE ROYAL SOCIETY OF CHEMISTRY, vol. 7, pages 4655 - 4659, XP002791407, DOI: 10.1039/C6FO00606J * |
XIAOYAN ZHOU ET AL.,: "Zeaxanthin improves diabetes-induced cognitive deficit in rats through activiting PI3K/AKT signaling pathway", BRAIN RESEARCH BULLETIN, vol. 132, pages 3 * |
Also Published As
Publication number | Publication date |
---|---|
JP7242219B2 (en) | 2023-03-20 |
JP2020033328A (en) | 2020-03-05 |
WO2020045647A1 (en) | 2020-03-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8569247B2 (en) | Hydrolysate of crocin | |
EP2226071B1 (en) | Composition for oral administration | |
JP6684966B2 (en) | Novel Lactobacillus sakei and composition containing the same | |
EP3974026A1 (en) | Composition for improving liver function | |
CN103652859A (en) | Health-care food with oxidation resistance and preparation method thereof | |
TWI627959B (en) | Use of lactobacillus reuteri gmnl-263 for manufacturing composition for increasing expression of ldl-r and cyp7a1 in liver in high-fat diet individual | |
JP2013176382A (en) | Composition for anti-fatigue | |
JP5926616B2 (en) | Aging inhibitor | |
JP5713332B2 (en) | Anti-fatigue composition | |
CN112566647A (en) | Blood sugar level increase inhibitor, diabetes inhibitor and food composition | |
KR20160043167A (en) | Method for seperating bee venom containing active amines and food composition thereof | |
KR101307051B1 (en) | Nature seasoning using micro algae protein hydrolysate and the manufacturing method thereof | |
JP6324551B2 (en) | Anti-breast cancer agent, food for anti-breast cancer, method for inhibiting breast cancer, and method for producing anti-breast cancer agent | |
JP7162542B2 (en) | Food composition for promoting food factor sensing-related gene expression and food factor sensing-related gene expression promoter | |
KR20140145666A (en) | Composition comprising natural complex of fucoxanthin, salix babylonica and low molecular weight alginate for preventing or treating of obesity | |
CN110404029B (en) | Composition with blood sugar reducing effect and preparation method and application thereof | |
Sharoba | Spirulina: Functional Compounds And Health Benefits | |
JP5577019B2 (en) | Orally administered composition | |
US11173187B2 (en) | Concentrated oil-based polyphenol composition and a method of producing the oil-based polyphenol composition | |
JP6050596B2 (en) | Nuclear receptor activity promoter and method for promoting nuclear receptor activity | |
JP6113092B2 (en) | Orally administered composition | |
JP7001450B2 (en) | Compositions and anti-inflammatory agents | |
KR20090021561A (en) | Composition for the prevention and treatment of fatty liver diseases containing honokiol as an active ingredient | |
US20090291153A1 (en) | Health food, Method for producing the same and dried Product and Extract | |
KR20220089191A (en) | Composition for preventing or improving fatty liver, comprising a black carrot extract as an active ingredient |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |