CN112553093B - Culture medium additive for improving organic selenium content in selenium yeast - Google Patents

Culture medium additive for improving organic selenium content in selenium yeast Download PDF

Info

Publication number
CN112553093B
CN112553093B CN202011467314.4A CN202011467314A CN112553093B CN 112553093 B CN112553093 B CN 112553093B CN 202011467314 A CN202011467314 A CN 202011467314A CN 112553093 B CN112553093 B CN 112553093B
Authority
CN
China
Prior art keywords
selenium
culture medium
yeast
medium additive
content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202011467314.4A
Other languages
Chinese (zh)
Other versions
CN112553093A (en
Inventor
张玉英
杨思林
魏春厅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anhui Huaxin Pharmaceutical Co ltd
Original Assignee
Anhui Huaxin Pharmaceutical Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anhui Huaxin Pharmaceutical Co ltd filed Critical Anhui Huaxin Pharmaceutical Co ltd
Priority to CN202011467314.4A priority Critical patent/CN112553093B/en
Publication of CN112553093A publication Critical patent/CN112553093A/en
Application granted granted Critical
Publication of CN112553093B publication Critical patent/CN112553093B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a culture medium additive for improving the content of organic selenium in selenium yeast, belonging to the technical field of selenium yeast. The culture medium additive comprises the following components: reduced Glutathione (GSH)1000mg/L, reduced nicotinamide adenine dinucleotide phosphate tetrasodium salt (NADPH-Na)4)100mg/L, 40g/L fructose and 800mg/L ATP 400-plus, orthogonal experiments are carried out on the addition amount of the four culture medium additives, and experiments show that the selenium-enriched yeast obtained after the additives are added into the culture medium has the characteristics of high biomass, high selenium content, high organic selenium conversion, high yield and the like, and the problem of low organic selenium conversion amount in the selenium-enriched yeast is effectively solved.

Description

Culture medium additive for improving organic selenium content in selenium yeast
Technical Field
The invention belongs to the technical field of selenium yeast, and particularly relates to a culture medium additive for improving the content of organic selenium in selenium yeast.
Background
Selenium (Se) is a trace element necessary for human bodies, is necessary for a plurality of proteins of the organisms to play physiological functions of maintaining the steady state of an immune system, delaying senescence and the like, and is very important for the health of the organisms by supplementing a proper amount of selenium every day, so that the selenium-rich selenium can not only prevent keshan disease and Kangshan disease, but also greatly reduce the risk of diseases of a cardiovascular system, an endocrine system, a nervous system, a reproductive system, a motion system and the like.
Selenium is divided into two forms of inorganic selenium and organic selenium, common inorganic selenium comprises sodium selenate and sodium selenite, and the inorganic selenium has low bioavailability and high toxicity to human bodies, so that a dietary supplement rich in organic selenium such as selenoprotein, selenium amino acid and the like is a main approach for supplementing selenium to human bodies, wherein selenium yeast is the most important and is the most easily available and most safe and effective source for supplementing selenium to human and animals at present.
At present, selenium yeast is derived from yeast cells cultured by fermentation in a medium containing inorganic selenium. In the fermentation culture process, the yeast cells can convert inorganic selenium in a culture medium into organic selenium per se, but the conversion capacity is limited, so that only selenium yeast with low organic selenium content can be obtained, the production performance is low, the practical application of the selenium yeast is limited, and the economic effect is low. Therefore, developing a method capable of effectively increasing the content of organic selenium in yeast cells is very important for the production and application of selenium yeast.
Disclosure of Invention
The invention provides a culture medium additive for improving the content of organic selenium in selenium yeast, aiming at solving the problem that the content of the organic selenium in the selenium yeast produced by the existing fermentation culture method is not high.
A culture medium additive for increasing the content of organic selenium in selenium yeast is prepared from the following substances: 1000mg reduced Glutathione (GSH), 100mg reduced nicotinamide adenine dinucleotide phosphate tetrasodium salt (NADPH-Na)4) 40g of fructose, 400-800 mg of Adenosine Triphosphate (ATP) and distilled water, and the volume is fixed to 1L.
The preparation method comprises the following steps: 1000mg of reduced Glutathione (GSH) and 100mg of reduced nicotinamide adenine dinucleotide phosphate tetrasodium salt (NADPH-Na) are added4) Respectively dissolving 40g of fructose and 400-800 mg of Adenosine Triphosphate (ATP) by using a small amount of distilled water completely, then mixing, and finally fixing the volume to 1L; obtaining an aqueous solution;
sterilizing the aqueous solution by using a filter sterilizer to obtain the culture medium additive for improving the content of organic selenium in the selenium yeast, and preparing the culture medium additive for use.
The technical scheme for further limiting is as follows:
the aperture of the filter membrane of the filter sterilizer is 0.22 micron.
The operation of the culture medium additive for improving the content of organic selenium in selenium yeast is as follows: inoculating the activated yeast strain into 1L of liquid culture medium according to the inoculation amount of 10%, culturing for 6-8 hours at the temperature of 28 ℃, adding sodium selenite until the final concentration is 50-70 mg/L, simultaneously adding 10mL of the culture medium additive into each liter of liquid culture medium, and continuously culturing for 24 hours to obtain the selenium yeast.
The liquid culture medium is prepared by dissolving 20g/L glucose, 10g/L yeast powder and 20g/L peptone in 1L distilled water and sterilizing at 121 ℃ for 20 minutes.
Compared with the prior art, the beneficial technical effects of the invention are embodied in the following aspects:
the invention effectively improves the capability of converting inorganic selenium into organic selenium of yeast cells, and the average content of the organic selenium in the yeast cells is more than 7 times of that of the organic selenium which is not added with the culture medium additive after fermentation culture for 24 hours in a liquid culture medium added with the culture medium additive.
The object of the present invention is achieved based on the following idea. Firstly, when yeast cells are fermented and cultured in a culture medium containing inorganic selenium, the inorganic selenium can generate peroxidation damage to the yeast cells, the growth and metabolic activity of the cells are inhibited, and the capacity of converting the inorganic selenium into organic selenium is further reduced, so that the peroxidation damage of the inorganic selenium to the growth and metabolic activity of the yeast cells is reduced when the selenium yeast is fermented and cultured; secondly, the ability of yeast cells to convert inorganic selenium into organic selenium is limited by the efficient enrichment of inorganic selenium in yeast cells and the adequate supply of reduced Glutathione (GSH), reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH) in the reaction of yeast cells to convert inorganic selenium into organic selenium. As GSH and NADPH are important auxiliary factors in the reaction of converting inorganic selenium into organic selenium by yeast cells, and are also important reducing agents for resisting peroxidation damage by the yeast cells, fructose can promote the inorganic selenium to enter cells from the outside so as to improve the substrate concentration of the inorganic selenium in the intracellular organic selenium generation reaction, and Adenosine Triphosphate (ATP) is an indispensable energy substance for absorbing and converting the organic selenium. The invention uses GSH and NADPH tetrasodium salt (NADPH-Na)4) Fructose and Adenosine Triphosphate (ATP) are used as raw materials, and a culture medium additive for improving the content of organic selenium in selenium yeast is developed.
In order to determine the composition of the culture medium additive, the invention adopts an orthogonal test method to determine GSH and NADPH-Na on the basis of single factor4Optimal formulation composition of fructose and ATP, factor level design of the four components is shown in table 1, and orthogonal experimental arrangement is shown in table 2.
TABLE 1L9(34) Factor level of orthogonal experiments
Figure BDA0002834833570000031
TABLE 2 orthogonal test arrangement and results and analysis
Figure BDA0002834833570000032
Figure BDA0002834833570000041
TABLE 3 analysis of variance in orthogonal tests
Figure BDA0002834833570000042
Figure BDA0002834833570000051
Preparing culture medium additive according to the arrangement of table 2, inoculating activated yeast strain into 1L fresh liquid culture medium according to the inoculation amount of 10%, culturing at 28 ℃ for 8 hours, adding sodium selenite to the final concentration of 62.5mg/L, simultaneously adding 10mL of culture medium additive, continuing culturing for 24 hours, harvesting selenium yeast, determining the growth amount, organic selenium content and total selenium content of the yeast, and determining the result in table 2 and the analysis of variance in table 3. As can be seen from Table 2, A3B1C1D2Is a theoretically optimal combination of the organic selenium content, A3B2C1D2Is the experimental optimal combination of the organic selenium content, and the theoretical optimal combination A3B1C1D2And experimentally optimum combination A3B2C1D2The results of the validation experiments are shown in Table 4.
TABLE 4 results of the verification test of the theoretical optimal combinations
Figure BDA0002834833570000052
The test results show that the optimal composition of the culture medium additive for improving the content of the organic selenium in the selenium yeast is as follows: reduced Glutathione (GSH)1000mg/L, reduced nicotinamide adenine dinucleotide phosphate-tetrasodium salt (NADPH-Na)4)100mg/L and 40g/L, ATP 800mg/L of fructose.
Secondly, peroxidation damage of inorganic selenium on growth of yeast cells is effectively reduced, the average growth amount of the yeast cells is averagely improved by 10 percent or more through fermentation culture for 24 hours in a culture medium added with a culture medium additive, a foundation for normal growth and metabolic activity is laid for converting the inorganic selenium into the organic selenium by the yeast cells, and the yield of the organic selenium and the total selenium in each liter of culture solution are respectively about 8.0 times and 7.5 times of that without the culture medium additive.
The culture medium additive is designed based on the cell biochemical metabolism principle, has the characteristics of accurate benefit, simple composition, easy preparation and convenient use, has the characteristics of improving the growth of yeast cells and the content of organic selenium, is used for the fermentation production of selenium yeast, has high yield, good quality and high benefit of the selenium yeast, and can meet the requirement of a human body on the organic selenium with less use amount.
Detailed Description
The present invention will be further described with reference to specific examples.
The features and advantages of the present invention will become more fully apparent from the following description, wherein it is shown and described only exemplary embodiments, rather than limitations on the scope of the invention, which are apparent to those skilled in the art from this detailed description, wherein it is shown and described that any and all methods, processes and articles of manufacture that embody the principles and novel and inventive features disclosed herein are within the scope of the invention.
The sources of the raw materials used in the following examples are illustrated below: the selenium yeast strain is provided by China Mobile Bio-drug industry, Inc. in Anhui province, and the organic selenium content of the selenium-rich yeast produced by the strain is less than 1000 mug/g dry weight of thallus; the liquid culture medium is purchased from Beijing Soilebao science and technology Co., Ltd; other chemical reagents such as sodium selenite and the like are domestic analytical pure.
Example 1
A culture medium additive for improving the content of organic selenium in selenium yeast is prepared by the following operations:
(1) 1000mg/L reduced Glutathione (GSH), 100mg/L reduced Nicotinamide Adenine Dinucleotide Phosphate (NADP), 40g/L fructose and 400mg/L Adenosine Triphosphate (ATP) were dissolved thoroughly in a small amount of distilled water, and the four substances were mixed uniformly and made into 1L aqueous solution.
(2) Sterilization
Sterilizing the above aqueous solution with a filter sterilizer with filter membrane pore size of 0.22 micrometer, and storing in a reagent bottle to obtain the culture medium additive for increasing organic selenium content in selenium yeast.
The use method of the culture medium additive comprises the following steps:
transferring the activated yeast with the inoculation amount of 10% into a fresh liquid culture medium containing 1L, performing shake culture at 28 ℃ and the oscillation speed of 180r/min for 8 hours, adding sodium selenite to the final concentration of 62.5mg/L, simultaneously adding 10mL of a culture medium additive, and continuing to culture for 24 hours to obtain the selenium yeast.
The liquid culture medium is prepared by dissolving 20g/L glucose, 10g/L yeast powder and 20g/L peptone in 1L distilled water, and sterilizing at 121 deg.C for 20 min.
Example 2
A culture medium additive for increasing the content of organic selenium in selenium yeast is prepared by the following steps:
(1) 1000mg/L reduced Glutathione (GSH), 100mg/L reduced Nicotinamide Adenine Dinucleotide Phosphate (NADP), 40g/L fructose and 800mg/L Adenosine Triphosphate (ATP) were dissolved thoroughly in a small amount of distilled water, and the four substances were mixed uniformly and made into 1L aqueous solution.
(2) Sterilization
Sterilizing the above aqueous solution with a filter sterilizer with filter membrane pore size of 0.22 micrometer, and storing in a reagent bottle to obtain the culture medium additive for increasing organic selenium content in selenium yeast.
The use method of the culture medium additive comprises the following steps:
transferring the activated yeast with the inoculation amount of 10% into a fresh liquid culture medium containing 1L, performing shake culture at 28 ℃ and the oscillation speed of 180r/min for 6 hours, adding sodium selenite to the final concentration of 51mg/L, simultaneously adding 10mL of culture medium additive, and continuing to culture for 24 hours to obtain the selenium yeast.
The liquid culture medium is prepared by dissolving 20g/L glucose, 10g/L yeast powder and 20g/L peptone in 1L distilled water, and sterilizing at 121 deg.C for 20 min.
Example 3
A culture medium additive for increasing the content of organic selenium in selenium yeast is prepared by the following steps:
(1) 1000mg/L reduced Glutathione (GSH), 100mg/L reduced Nicotinamide Adenine Dinucleotide Phosphate (NADP), 40g/L fructose and 600mg/L Adenosine Triphosphate (ATP) are respectively and completely dissolved by using a small amount of distilled water, and the four substances are uniformly mixed and are contained into 1L of aqueous solution.
(2) Sterilization
Sterilizing the above aqueous solution with a filter sterilizer with filter membrane aperture of 0.22 micrometer, and storing in a reagent bottle to obtain the culture medium additive for increasing organic selenium content in selenium yeast.
The use method of the culture medium additive comprises the following steps:
transferring the activated yeast with the inoculation amount of 10% into a fresh liquid culture medium containing 1L, performing shake culture at 28 ℃ and the oscillation speed of 180r/min for 7 hours, adding sodium selenite to the final concentration of 70mg/L, simultaneously adding 10mL of culture medium additive, and continuing to culture for 24 hours to obtain the selenium yeast.
The liquid culture medium is prepared by dissolving 20g/L glucose, 10g/L yeast powder and 20g/L peptone in 1L distilled water, and sterilizing at 121 deg.C for 20 min.
The biomass, total selenium content, organic selenium yield and organic selenium ratio of the selenium yeast of examples 1 to 3 were measured, respectively, and the results are shown in Table 5.
Comparative example:
(1) transferring the activated yeast with the inoculation amount of 10% into a fresh liquid culture medium containing 1L, performing shake culture at 28 ℃ and the oscillation speed of 180r/min for 8 hours, adding sodium selenite to the final concentration of 62.5mg/L, simultaneously adding 10mL of a culture medium additive, and continuously culturing for 24 hours to obtain the selenium yeast.
(2) The biomass, total selenium content, organic selenium yield and organic selenium ratio of the strains were determined and the results are shown in table 5.
TABLE 5 analysis of characteristics of selenium Yeast in examples 1-3 and comparative examples
Figure BDA0002834833570000071
Figure BDA0002834833570000081
As can be seen from Table 5, the selenium yeast, when cultured in the medium containing the additive of the present invention, not only increased the biomass, but also significantly increased the total selenium concentration and the organic selenium concentration in the cells per unit mass, and increased the organic selenium ratio by more than 10%.
The above examples are only for illustrating the technical idea and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, and not to limit the protection scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the protection scope of the present invention.

Claims (3)

1. A culture medium additive for improving the content of organic selenium in selenium yeast is characterized in that: the culture medium additive is prepared from the following substances: 1000mg of reduced glutathione, 100mg of reduced nicotinamide adenine dinucleotide phosphate tetrasodium salt, 40g of fructose, 400-800 mg of adenosine triphosphate and distilled water, and fixing the volume to 1L;
firstly, completely dissolving 1000mg of reductive glutathione, 100mg of reductive nicotinamide adenine dinucleotide phosphate tetrasodium salt, 40g of fructose and 400-800 mg of adenosine triphosphate by using a small amount of distilled water respectively, then mixing, and finally fixing the volume to 1L; obtaining an aqueous solution;
sterilizing the aqueous solution with a filter sterilizer to obtain the culture medium additive, and preparing the culture medium additive for use.
2. The culture medium additive for increasing the content of organic selenium in selenium yeast as claimed in claim 1, wherein: the aperture of the filter membrane of the filter sterilizer is 0.22 micron.
3. The method for using the culture medium additive for improving the content of the organic selenium in the selenium yeast as claimed in claim 1, is characterized in that: inoculating the activated yeast strain into 1L of liquid culture medium according to the inoculation amount of 10%, culturing for 6-8 hours at the temperature of 28 ℃, adding sodium selenite until the final concentration is 50-70 mg/L, simultaneously adding 10mL of the culture medium additive into each liter of liquid culture medium, and continuously culturing for 24 hours to obtain selenium yeast;
the liquid culture medium is prepared by dissolving 20g/L glucose, 10g/L yeast powder and 20g/L peptone in 1L distilled water and sterilizing at 121 ℃ for 20 minutes.
CN202011467314.4A 2020-12-14 2020-12-14 Culture medium additive for improving organic selenium content in selenium yeast Active CN112553093B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011467314.4A CN112553093B (en) 2020-12-14 2020-12-14 Culture medium additive for improving organic selenium content in selenium yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011467314.4A CN112553093B (en) 2020-12-14 2020-12-14 Culture medium additive for improving organic selenium content in selenium yeast

Publications (2)

Publication Number Publication Date
CN112553093A CN112553093A (en) 2021-03-26
CN112553093B true CN112553093B (en) 2022-06-21

Family

ID=75064512

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011467314.4A Active CN112553093B (en) 2020-12-14 2020-12-14 Culture medium additive for improving organic selenium content in selenium yeast

Country Status (1)

Country Link
CN (1) CN112553093B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140107A (en) * 1996-09-25 2000-10-31 Viva America Marketing, Inc. Organometallic-metabolizing yeast
WO2006103350A1 (en) * 2005-03-30 2006-10-05 Lallemand Sas Yeast preparations with improved antioxidant properties and uses thereof
CN107699505A (en) * 2017-10-19 2018-02-16 四川农业大学 A kind of optimization method and technique of Se-enriched yeast culture process
CN111979171A (en) * 2020-08-07 2020-11-24 青岛科海生物有限公司 Method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140107A (en) * 1996-09-25 2000-10-31 Viva America Marketing, Inc. Organometallic-metabolizing yeast
WO2006103350A1 (en) * 2005-03-30 2006-10-05 Lallemand Sas Yeast preparations with improved antioxidant properties and uses thereof
CN107699505A (en) * 2017-10-19 2018-02-16 四川农业大学 A kind of optimization method and technique of Se-enriched yeast culture process
CN111979171A (en) * 2020-08-07 2020-11-24 青岛科海生物有限公司 Method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Bioaccumulation of selenium in saccharomyces-cerevisiae cells;Danch A 等;《Prace Naukowe Uniwersytetu Slaskiego w Katowicach》;19851231;第57-64页 *
富硒酵母菌的筛选及其富硒条件的优化;朱威等;《安徽农业科学》;20171231(第33期);第87-89、92页 *
酸胁迫在提升富硒/GSH产朊假丝酵母性能中的作用;王大慧等;《中国生物工程杂志》;20131115(第11期);第81-85页 *

Also Published As

Publication number Publication date
CN112553093A (en) 2021-03-26

Similar Documents

Publication Publication Date Title
CN106929422B (en) A kind of method of chlorella and yeast co-cultivation purification yeast wastewater
CN101407774B (en) Preparation technique of photosynthetic bacteria preparation
CN106282044B (en) A kind of preparation method of Hyphomicrobium and pyrroloquinoline quinone
US10053744B2 (en) Yeast strain with high yield of glutathione
CN106399429A (en) Method for improving yield of adenosine through feeding and mixing nitrogen source
CN110791462B (en) Bacillus subtilis and application thereof in fermentation production of adenosine
CN114214216B (en) Selenium-enriched yeast strain and application thereof
CN101948878B (en) Application of gluconobacter oxydans in preparing 1,3-dioxyacetone
CN112553093B (en) Culture medium additive for improving organic selenium content in selenium yeast
CN105498718B (en) A kind of biosurfactant functional modification D. radiodurans DR method and its application
RU2670014C2 (en) Method for microbial fermentation of sugary substrates and use of hydrogen in atomic, ionic or gaseous state in such method
Liu et al. Selection of a high-biomass, chromium-rich yeast strain and optimization of cultivation conditions
CN111979171B (en) Method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquor
CN115926991A (en) Method for reducing diatom shell solubility, low-solubility diatom and application
CN116396882A (en) NRK producing strain, method for producing NRK and application
CN112262939A (en) Method for increasing acid production rate in preparation of brown lactobacillus beverage
CN114703243A (en) Method for producing adenosine by fermentation
CN101497871B (en) Alcohol fermentation anaerobic high temperature bacterium culture medium, preparation and use thereof
CN110510752B (en) Microbial growth-promoting synergistic nutrient and preparation method and application thereof
CN112980715A (en) Bacillus subtilis strain B13 and application thereof
CN114134056A (en) Saccharomyces cerevisiae ZJS10041 and application thereof in fermentation production of S-adenosylmethionine
CN106755129B (en) Method for producing ethanol by fermenting immobilized yeast cells under waste molasses raw material
CN113817654B (en) Fermentation medium and fermentation method for producing riboflavin
CN113699141B (en) Sustained-release carbon source immobilized aerobic denitrification composite microbial inoculant and preparation method and application thereof
CN107090483B (en) Application of saccharomyces cerevisiae in glutathione biosynthesis

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant