CN114703243A - Method for producing adenosine by fermentation - Google Patents
Method for producing adenosine by fermentation Download PDFInfo
- Publication number
- CN114703243A CN114703243A CN202111661509.7A CN202111661509A CN114703243A CN 114703243 A CN114703243 A CN 114703243A CN 202111661509 A CN202111661509 A CN 202111661509A CN 114703243 A CN114703243 A CN 114703243A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- adenosine
- seed
- culture medium
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 99
- 230000004151 fermentation Effects 0.000 title claims abstract description 99
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 title claims abstract description 78
- 239000002126 C01EB10 - Adenosine Substances 0.000 title claims abstract description 39
- 229960005305 adenosine Drugs 0.000 title claims abstract description 39
- 238000004519 manufacturing process Methods 0.000 title abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 35
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 239000008103 glucose Substances 0.000 claims abstract description 33
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000001963 growth medium Substances 0.000 claims abstract description 20
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 15
- 239000012138 yeast extract Substances 0.000 claims abstract description 15
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 13
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 13
- 239000004202 carbamide Substances 0.000 claims abstract description 13
- 229960001031 glucose Drugs 0.000 claims description 33
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 26
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 22
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 22
- 238000012258 culturing Methods 0.000 claims description 19
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 17
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 17
- 239000002609 medium Substances 0.000 claims description 14
- 244000063299 Bacillus subtilis Species 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 13
- 239000011780 sodium chloride Substances 0.000 claims description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 11
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 11
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 11
- 229940099596 manganese sulfate Drugs 0.000 claims description 11
- 239000011702 manganese sulphate Substances 0.000 claims description 11
- 235000007079 manganese sulphate Nutrition 0.000 claims description 11
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 11
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 11
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 11
- 239000001103 potassium chloride Substances 0.000 claims description 11
- 235000011164 potassium chloride Nutrition 0.000 claims description 11
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 11
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- SPFMQWBKVUQXJV-BTVCFUMJSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;hydrate Chemical compound O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O SPFMQWBKVUQXJV-BTVCFUMJSA-N 0.000 claims description 10
- 229960000673 dextrose monohydrate Drugs 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 5
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 238000012262 fermentative production Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 description 29
- 238000004659 sterilization and disinfection Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 13
- 230000001580 bacterial effect Effects 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- 238000010899 nucleation Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 150000003833 nucleoside derivatives Chemical class 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 2
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 2
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 2
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- GRSZFWQUAKGDAV-KQYNXXCUSA-L IMP(2-) Chemical compound O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(N=CNC2=O)=C2N=C1 GRSZFWQUAKGDAV-KQYNXXCUSA-L 0.000 description 2
- 239000012880 LB liquid culture medium Substances 0.000 description 2
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 210000004165 myocardium Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 102000004008 5'-Nucleotidase Human genes 0.000 description 1
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 description 1
- 102000055025 Adenosine deaminases Human genes 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- 206010052895 Coronary artery insufficiency Diseases 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000007530 Essential hypertension Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 208000003734 Supraventricular Tachycardia Diseases 0.000 description 1
- 206010042600 Supraventricular arrhythmias Diseases 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 description 1
- 108010058516 adenosine phosphorylase Proteins 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical class N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000000916 dilatatory effect Effects 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 150000004682 monohydrates Chemical class 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 201000008752 progressive muscular atrophy Diseases 0.000 description 1
- 108010043671 prostatic acid phosphatase Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 208000002320 spinal muscular atrophy Diseases 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000005062 synaptic transmission Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/38—Nucleosides
- C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a method for producing adenosine by fermentation, which relates to a deep liquid fermentation culture medium formula, a fermentation control method, a feeding method and the like for producing adenosine by using the strain, and the color purity level of the finally obtained fermentation liquor is close to 90%, which is beneficial to extracting and purifying to obtain the adenosine finished product of pharmacopoeia standard. According to the method, deep liquid fermentation is carried out by using high-concentration glucose as a carbon source and yeast extract, urea, ammonium sulfate and the like as nitrogen sources, the level of a 50L fermentation tank can reach 11.2g/L, and the level of a 1T fermentation tank can reach 10.5 g/L.
Description
Technical Field
The invention belongs to the field of microbial fermentation, and particularly relates to a method for producing adenosine by fermentation.
Background
Adenosine (Adenosine), the chemical name of which is 6-amino-9-beta-D-ribofuranosyl-9-hydropurine, belongs to an important nucleotide derivative and is a dephosphorylated product of adenine nucleotide. As endogenous nucleoside distributed throughout human body cells, the nucleoside can directly enter cardiac muscle to generate adenosine through phosphorylation, participates in cardiac muscle energy metabolism, and also participates in dilating coronary blood vessels to increase blood flow. Can be used for treating supraventricular tachycardia, supraventricular arrhythmia related to atrioventricular, angina pectoris, myocardial infarction, coronary insufficiency, arteriosclerosis, essential hypertension, cerebrovascular disorder, apoplexy sequela, and progressive muscular atrophy. Adenosine has physiological effects on the cardiovascular system and many other systems and tissues of the body. Adenosine plays a biochemical important role, including the transfer of energy in the form of Adenosine Triphosphate (ATP) or Adenosine Diphosphate (ADP), or the signaling of cyclic adenosine monophosphate (cAMP), among others. In addition, adenosine is an inhibitory neurotransmitter, and plays an important role in neurotransmission.
Currently, the methods used in the prior art for the production of adenosine consist of RNA hydrolysis, chemical methods and fermentation methods. The chemical synthesis method for synthesizing the adenosine has a series of problems of high cost, complicated reaction, low yield and the like. The RNA hydrolysis method can simultaneously obtain 4 nucleoside substances, which brings difficulty to subsequent separation. In comparison, the microbial fermentation method has the advantages of low cost, easily available raw materials and high adenosine production efficiency, thereby occupying absolute advantages in adenosine production.
However, there are also many problems in adenosine fermentation. One is that adenosine mostly adopts genetic engineering strains or strains after physical and chemical mutagenesis as production strains, for example, the adenosine fermentation strain of bacillus subtilis has the following characteristics: inosinate dehydrogenase is absent, i.e. xanthine deficient; adenosine deaminase is absent, cutting the pathway from adenylate to inosinate. Adenosine phosphorylase is absent, and adenosine decomposition is reduced; releasing the feedback inhibition of the adenosine monophosphate final product pathway enzyme. 5' -nucleotidase has high activity, and adenosine is rapidly decomposed into adenosine. However, the inventor finds that the auxotrophic strain generally has the problems of unstable hereditary character and yield and the like, has serious strain degeneration phenomenon, higher reversion rate and poor product quality stability, and is not beneficial to industrial production. The glucose tolerance of the bacteria disclosed by the prior art is not high, the glucose tolerance is an important index of industrial application prospects of the strains, the strains with better glucose tolerance can not only tolerate high-concentration substrates, but also tolerate high-concentration osmotic pressure, and the method has important significance for improving the concentration of products. Secondly, adenosine is used as an injection for development, the quality requirement on raw materials is very high, and the problem of low adenosine fermentation purity of original strains generally exists, so that the downstream process is complicated, and the purification cost is high. Thirdly, adenosine fermentation needs to provide very rich nutrition (organic nitrogen source), so that the cost of adenosine fermentation is very high. Fourthly, reported high-level adenosine-producing bacteria capable of being inquired only exist at a laboratory level, and do not reach a large production stage, and industrial amplification is a very difficult point for genetic engineering bacteria, so that it is very necessary to develop a fermentation method for stably producing adenosine and to be capable of stably amplifying the adenosine.
Disclosure of Invention
The invention aims to provide a fermentation method which can stably produce adenosine and can stably realize industrial amplification.
A method for the fermentative production of adenosine, comprising the steps of: taking Bacillus subtilis for producing adenosine as fermentation strain, and controlling glucose concentration of fermentation liquid to be 5-60g/L, such as 5-10g/L, 10-20g/L, 20-30g/L, 30-40g/L, 40-50g/L, and 50-60g/L in the fermentation process; the pH is 6.1-7.3, e.g., 6.1, 6.2, 6.2, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3.
As a specific embodiment, the glucose concentration is 10-60g/L, preferably 20-60g/L, more preferably 30-60g/L, such as 30-40g/L, 40-50g/L, 50-60 g/L.
As a specific embodiment, the glucose supplementation mode is pulse supplementation.
Further, preferably, when the glucose concentration is less than 10 to 15g/L, glucose is supplemented so that the glucose concentration is 80 to 120 g/L.
As a specific embodiment, the pH is preferably 6.3 to 6.4, and the pH is adjusted by feeding ammonia water.
As a specific implementation mode, the fermentation strain adopts Bacillus subtilis HDCC112-21027 which is preserved in China general microbiological culture Collection center (CGMCC), the preservation number is CGMCC NO.23448, and the preservation date is 2021, 09 months and 18 days.
As a specific embodiment, the bacillus subtilis is prepared by inoculating a seed solution into the fermentation culture medium by an inoculation amount of 5-20% (V/V) for fermentation culture, controlling the temperature to be 25-40 ℃, controlling the dissolved oxygen to be more than or equal to 10-40%, and controlling the total fermentation time to be 48-64 h.
As a specific embodiment, the liquid fermentation medium formula comprises the following components: 5-240g/L of dextrose monohydrate, 0-50g/L of yeast extract, 0-50g/L of yeast powder, 0-30g/L of ammonium sulfate, 0-7g/L of monopotassium phosphate, 0-5g/L of potassium chloride, 0-0.05g/L of manganese sulfate, 0-14g/L of urea, 0-5g/L of magnesium sulfate and 0-1g/L of sodium chloride, wherein 0 is not selected from 0.
Further, it is preferred that the liquid fermentation medium comprises the following components: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 30g/L of yeast powder, 10g/L of ammonium sulfate, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate, 0.5g/L of sodium chloride and ammonia water for adjusting the pH value to 7.0.
Further, as a specific embodiment, the seed solution is obtained by seed culturing bacillus subtilis in a seed culture medium; inoculating qualified liquid seeds in a ratio of 0.1-2.0% (V/W) to a liquid fermentation medium, controlling the temperature at 36 + -1 deg.C and the dissolved oxygen at 10-40%, culturing for 5-20h, and controlling the OD of the seed liquid to be not less than 8.0; the culture medium in the seed culture process is an LB culture medium, 5g/L of an LB culture medium yeast extract, 10g/L of peptone, 10g/L of sodium chloride and 10g/L of glucose, and the pH value is adjusted to 7.0.
The patent provides a method for producing adenosine by high-concentration glucose-resistant bacillus subtilis, and relates to a deep liquid fermentation culture medium formula, a fermentation control method, a material supplementing method and the like for producing adenosine by the bacillus subtilis, and the color purity level of the finally obtained fermentation liquor is close to 90%, so that the extraction and purification are facilitated to obtain the adenosine finished product with pharmacopoeia standards. According to the method, deep liquid fermentation is carried out by using high-concentration glucose as a carbon source and yeast extract, urea, ammonium sulfate and the like as nitrogen sources, so that the level of a 50L fermentation tank can reach 11.2g/L, and the level of a 1T fermentation tank can reach 10.5 g/L.
The bacterial strain for producing high-purity adenosine by adopting the high-concentration glucose-resistant bacillus subtilis is unique in the adenosine production bacterial strain disclosed at present, compared with low-concentration sugar of other disclosed bacterial strains, the color purity of fermentation liquor is low, the subsequent purification is not facilitated, and the bacterial strain has the advantages of wide sugar-resistant concentration range, difficulty in bacterial contamination, simple control process, high color purity of final fermentation liquor and the like in comparison of fine control process, and can realize industrial production.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the present invention will be described more clearly and completely below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The Bacillus subtilis HDCC112-21027 in the embodiment is preserved in China general microbiological culture Collection center (CGMCC), the preservation number is CGMCC NO.23448, and the preservation date is 2021, 09 months and 18 days. The fermentation liquor obtained in the embodiment of the invention refers to the second part of China pharmacopoeia 2020 edition for liquid chromatography analysis and measurement.
Example 1
1. And (3) recovery and activation of strains: thawing a working strain glycerin tube (CGMCC NO.23448) at room temperature, sucking 0.1ml of bacterial suspension, inoculating the bacterial suspension to an LB solid plate, uniformly coating, and culturing in an incubator at 35 ℃ for 24 hours to obtain an activated and revived single bacterial colony.
2. Preparing liquid seeds: and (3) taking activated and revived single colonies, picking one single colony by using an inoculating shovel, inoculating the single colony into a 500ml triangular flask containing 100ml of LB liquid culture medium, bundling, placing on a shaking table at 35 ℃ and 250rpm, and performing shaking culture for 20 hours, wherein the OD value of the seed liquid is controlled to be more than or equal to 0.5. Adjusting the pH value of the LB liquid culture medium to 7.0 before disinfection, wherein the disinfection condition is 121-123 ℃ and 30 min.
3. Seed tank seed culture: inoculating qualified liquid seeds at a ratio of 0.5% (V/W) into a 50L automatic control fermentation tank filled with 20L liquid fermentation medium, controlling the temperature at 36 deg.C and the dissolved oxygen at more than 20%, and adjusting air flow and stirring speed. Culturing for 9h on a tank, controlling the OD of the seed solution to be more than or equal to 8.0, adjusting the pH to 7.0 before disinfection by using an LB culture medium in the seed tank, and sterilizing for 30min at the temperature of 121-123 ℃.
4. Culturing in a fermentation tank: inoculating qualified seed in seeding tank at 10% (V/V) into 50L self-control fermentation tank filled with 25L liquid fermentation medium, controlling temperature at 36 deg.C and controlling dissolved oxygen at 20% or more, and regulating air flow and stirring speed. The glucose concentration of the fermentation liquor is controlled to be 30-40g/L in the process, the pH value of ammonia water fed-batch automatic control is 6.3-6.4, the fermentation is carried out for 48h, and the fermentation is put into a tank, and the formula of the liquid fermentation culture medium comprises the following components: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 10g/L of ammonium sulfate of yeast powder, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride, adjusting the pH value to 7.0 by ammonia water after disinfection, and carrying out 30min under the disinfection conditions of 121-123 ℃.
5. Sample treatment and detection: after fermentation is finished, 2ml of fermentation liquor is taken, high-speed centrifugation is carried out at 10000rpm for 10min to remove thalli, then supernatant is taken and filtered by a 0.45-micron microporous filter membrane, liquid chromatography analysis and measurement are carried out according to the second part of 'Chinese pharmacopoeia' 2020 edition, the final titer level reaches 11.2g/L, and the purity is 90.1%.
Example 2
1. Seed solution preparation same as example 1;
2. culturing in a fermentation tank: inoculating qualified seed in seeding tank at 10% (V/V) into 50L self-control fermentation tank filled with 25L liquid fermentation medium, controlling temperature at 36 deg.C and controlling dissolved oxygen at 20% or more, and regulating air flow and stirring speed. The glucose concentration of the fermentation liquor is controlled to be 5-10g/L, 10-20g/L, 20-30g/L, 30-40g/L, 40-50g/L and 50-60g/L respectively, and the pH value is automatically controlled to be 6.3-6.4 by ammonia water feeding. Culturing for 48h and placing in a tank.
3. The liquid fermentation culture medium comprises the following components in parts by weight: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 10g/L of ammonium sulfate of yeast powder, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride. Adjusting the pH value of the ammonia water to 7.0 after disinfection, wherein the disinfection condition is 121-123 ℃ and 30 min. The final tank discharge level is shown in the following table 1, and the purity is between 85 and 91 percent.
TABLE 1 fermentation levels for different glucose concentrations
| Glucose concentration (g/L) | Fermentation level (g/L) |
| 5-10 | 5.4 |
| 10-20 | 5.3 |
| 20-30 | 7.2 |
| 30-40 | 11.2 |
| 40-50 | 10.4 |
| 50-60 | 9.8 |
Example 3
1. Seed solution preparation the same as example 1;
2. culturing in a fermentation tank: inoculating qualified seed in seeding tank at 10% (V/V) into 50L self-control fermentation tank filled with 25L liquid fermentation medium, controlling temperature at 36 deg.C and controlling dissolved oxygen at 20% or more, and regulating air flow and stirring speed. Controlling the glucose concentration of the fermentation liquor to be 30-40g/L in the process, feeding ammonia water, automatically controlling the pH value to be 5.5, 5.8, 6.1, 6.4, 6.7, 7.0 and 7.3, culturing for 48h, and putting into a tank; the liquid fermentation culture medium comprises the following components in parts by weight: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 10g/L of ammonium sulfate of yeast powder, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride. Adjusting the pH value of the ammonia water to 7.0 after disinfection, wherein the disinfection conditions are 121-123 ℃ and 30min, and the final tank placing level is shown in the following table 2, and the purity is between 85-91%.
TABLE 2 fermentation levels at different pH
| pH | Fermentation level (g/L) |
| 5.5 | 0.3 |
| 5.8 | 1.2 |
| 6.1 | 6.8 |
| 6.4 | 10.9 |
| 6.7 | 9.9 |
| 7.0 | 8.4 |
| 7.3 | 5.3 |
Example 4
1. Seed solution preparation the same as example 1;
2. culturing in a fermentation tank: inoculating qualified seed in seeding tank at 10% (V/V) into 50L self-control fermentation tank filled with 25L liquid fermentation medium, controlling temperature at 36 deg.C and controlling dissolved oxygen at 20% or more, and regulating air flow and stirring speed. Controlling the glucose concentration of the fermentation liquor to be 30-40g/L in the process, feeding 30% sodium hydroxide solution, automatically controlling the pH to be 6.4, culturing for 48h, and putting into a tank; the liquid fermentation culture medium comprises the following components in parts by weight: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 10g/L of ammonium sulfate of yeast powder, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride. Adjusting the pH value of the ammonia water to 7.0 after disinfection, wherein the disinfection condition is 121-123 ℃ and 30min, and the final tank placing level is 7.2g/L, and the purity is 85.7%.
Example 5
1. Seed solution preparation same as example 1;
2. culturing in a fermentation tank: inoculating qualified seed in seed tank at a ratio of 2%, 5%, 10%, 15%, and 20% (V/V) into a 50L self-control fermentation tank containing 25L liquid fermentation medium, controlling temperature at 36 deg.C, controlling dissolved oxygen at 20% or more, and adjusting air flow and stirring speed. The glucose concentration of the fermentation liquor is controlled to be 30-40g/L in the process, and the pH value of the ammonia water solution is automatically controlled to be 6.4. Culturing for 48h and placing in a tank; the liquid fermentation culture medium comprises the following components in parts by weight: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 30g/L of yeast powder, 10g/L of ammonium sulfate, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride. Adjusting the pH value of the ammonia water to 7.0 after disinfection, wherein the disinfection condition is 121-123 ℃ and 30 min. The final tank discharge level is shown in the following table 3, and the purity is between 85 and 91 percent.
TABLE 3 fermentation levels for different inoculum size
| The seed transferring amount is% | Fermentation level (g/L) |
| 2 | 1.1 |
| 5 | 7.8 |
| 10 | 10.9 |
| 15 | 10.5 |
| 20 | 9.9 |
Example 6
1. Seed solution preparation the same as example 1;
2. culturing in a fermentation tank: inoculating qualified seed in seeding tank at 10% (V/V) into 50L self-control fermentation tank filled with 25L liquid fermentation medium, controlling temperature at 36 deg.C and controlling dissolved oxygen at 20% or more, and regulating air flow and stirring speed. Controlling the glucose concentration of the fermentation liquor to be 30-40g/L in the process, automatically controlling the pH value to be 6.4 by adding ammonia water solution, culturing for 48h, and putting into a tank; the liquid fermentation culture medium comprises the following components in parts by weight: the concentration of the monohydrate dextrose is taken as a gradient, 20g/L of yeast extract, 10g/L of yeast powder ammonium sulfate, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride. Adjusting the pH value of the ammonia water to 7.0 after disinfection, wherein the disinfection conditions are 121-123 ℃ and 30min, and the final tank placing level is shown in the following table 4, and the purity is between 85-91%.
TABLE 4 fermentation levels for different basal glucose concentrations
| Basal glucose concentration g/L | Fermentation level (g/L) |
| 60 | 7.8 |
| 100 | 8.1 |
| 140 | 9.9 |
| 180 | 11.0 |
| 220 | 8.8 |
| 260 | 4.4 |
Example 7
1. Seed liquid was prepared as in example 1.
2. Culturing in a fermentation tank: inoculating qualified seeding tank seeds into a 50L automatic control fermentation tank filled with 25L liquid fermentation medium at a ratio of 10% (V/V), controlling the temperature at 36 deg.C, controlling the dissolved oxygen content at 20% or more, and adjusting air flow and stirring speed. The glucose concentration control mode of the process control fermentation liquor is as follows: the glucose concentration is lower than 10g/L, glucose is added once to enable the glucose concentration of the fermentation liquor to reach 80-120g/L, and the action is repeated after the glucose is consumed. Feeding ammonia water solution, automatically controlling the pH value to be 6.4, culturing for 48h, and putting into a tank; the liquid fermentation culture medium comprises the following components in parts by weight: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 10g/L of ammonium sulfate of yeast powder, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate and 0.5g/L of sodium chloride. Adjusting the pH value of the ammonia water to 7.0 after disinfection, wherein the disinfection condition is 121-123 ℃ and 30min, and the final tank placing level is 10.5g/L, and the purity is 92%.
Claims (10)
1. A method for the fermentative production of adenosine, comprising the steps of: takes the adenosine-producing bacillus subtilis as a fermentation strain, and controls the glucose concentration of fermentation liquor to be 5-60g/L and the pH value to be 6.1-7.3 in the fermentation process.
2. The method according to claim 1, wherein the glucose concentration is 10-60g/L, preferably 20-60g/L, more preferably 30-60 g/L.
3. The method of claim 1, wherein: the glucose is supplemented in a pulse mode.
4. The method of claim 1, wherein: the pH is preferably 6.3-6.4, and the pH is adjusted by adding ammonia water in a flowing manner.
5. The method of claim 1, wherein: the fermentation strain adopts Bacillus subtilis HDCC112-21027, is preserved in China general microbiological culture Collection center (CGMCC), has a preservation number of CGMCC NO.23448, and has a preservation date of 2021 years, 09 months and 18 days.
6. The method of claim 1 or 2, wherein the bacillus subtilis is cultured by inoculating a seed solution into the fermentation medium at an inoculation amount of 5-20% (V/V) for fermentation at 25-40 ℃ under dissolved oxygen of 10-40% or more for a total fermentation time of 48-64 hr.
7. The method of any one of claims 1 to 6, wherein the liquid fermentation medium formulation comprises the following components: 5-240g/L of dextrose monohydrate, 0-50g/L of yeast extract, 0-50g/L of ammonium sulfate, 0-30g/L of yeast powder, 0-7g/L of monopotassium phosphate, 0-5g/L of potassium chloride, 0-0.05g/L of manganese sulfate, 0-14g/L of urea, 0-5g/L of magnesium sulfate and 0-1g/L of sodium chloride.
8. The method of claim 7, wherein the liquid fermentation medium comprises the following components: 180g/L of dextrose monohydrate, 20g/L of yeast extract, 30g/L of yeast powder, 10g/L of ammonium sulfate, 3.5g/L of monopotassium phosphate, 2g/L of potassium chloride, 0.02g/L of manganese sulfate, 7g/L of urea, 2g/L of magnesium sulfate, 0.5g/L of sodium chloride and ammonia water for adjusting the pH value to 7.0.
9. The method of claim 6, wherein the seed solution is obtained by seed culturing Bacillus subtilis in a seed culture medium; inoculating qualified liquid seeds at a ratio of 0.1-2.0% (V/W) into a liquid fermentation culture medium, controlling the temperature at 36 + -2 deg.C and the dissolved oxygen at 10-40%, culturing for 5-20h, and controlling the OD of the seed liquid at 8.0 or more.
10. The method of claim 9, wherein: the culture medium in the seed culture process is an LB culture medium, and the pH is adjusted to 7.0; the LB culture medium is 5g/L yeast extract, 10g/L peptone, 10g/L sodium chloride and 10g/L glucose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111661509.7A CN114703243A (en) | 2021-12-31 | 2021-12-31 | Method for producing adenosine by fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111661509.7A CN114703243A (en) | 2021-12-31 | 2021-12-31 | Method for producing adenosine by fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114703243A true CN114703243A (en) | 2022-07-05 |
Family
ID=82167314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111661509.7A Pending CN114703243A (en) | 2021-12-31 | 2021-12-31 | Method for producing adenosine by fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114703243A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676409A (en) * | 2012-03-09 | 2012-09-19 | 南京工业大学 | Brewer's yeast and process for producing S-adenosylmethionine by fed-batch fermentation |
| CN103146786A (en) * | 2013-03-25 | 2013-06-12 | 天津科技大学 | Method for producing adenosine by sequentially controlling fermentation with gradient pH |
| CN110791462A (en) * | 2019-12-16 | 2020-02-14 | 江苏澳创生物科技有限公司 | Bacillus subtilis and application thereof in fermentation production of adenosine |
| CN111254172A (en) * | 2019-12-10 | 2020-06-09 | 新疆阜丰生物科技有限公司 | Method for producing adenosine by fermentation |
-
2021
- 2021-12-31 CN CN202111661509.7A patent/CN114703243A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676409A (en) * | 2012-03-09 | 2012-09-19 | 南京工业大学 | Brewer's yeast and process for producing S-adenosylmethionine by fed-batch fermentation |
| CN103146786A (en) * | 2013-03-25 | 2013-06-12 | 天津科技大学 | Method for producing adenosine by sequentially controlling fermentation with gradient pH |
| CN111254172A (en) * | 2019-12-10 | 2020-06-09 | 新疆阜丰生物科技有限公司 | Method for producing adenosine by fermentation |
| CN110791462A (en) * | 2019-12-16 | 2020-02-14 | 江苏澳创生物科技有限公司 | Bacillus subtilis and application thereof in fermentation production of adenosine |
Non-Patent Citations (1)
| Title |
|---|
| XIAOCHUN CHEN，等: "Enhancement of adenosine production by Bacillus subtilis CGMCC 4484 through metabolic flux analysis and simplified feeding strategies", 《BIOPROCESS BIOSYST ENG》, no. 36, 31 December 2013 (2013-12-31), pages 1851 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11149048B2 (en) | High-purity D-psicose preparation method | |
| CN106399429A (en) | Method for improving yield of adenosine through feeding and mixing nitrogen source | |
| CN101173308A (en) | Method for ferment for producing adenomethionine with genetic engineering bacterium | |
| CN102533889B (en) | Method for continuously fermenting lysine | |
| CN108285915B (en) | Fermentation method of gibberellic acid | |
| CN106929548B (en) | Process for producing malic acid by fermenting aspergillus oryzae | |
| CN110791462B (en) | Bacillus subtilis and application thereof in fermentation production of adenosine | |
| CN112940945B (en) | Method for fermenting hirsutella sinensis | |
| CN101437934B (en) | Method of producing dry yeast containing s-adenosyl-l-methionine and composition for oral intake | |
| CN110283854B (en) | Fermentation medium, application thereof and method for preparing lycopene by utilizing Blakeslea trispora fermentation | |
| CN112501221A (en) | Method for improving conversion rate of threonine and saccharic acid | |
| CN112795607A (en) | Method for improving adenosine fermentation yield | |
| CN114703243A (en) | Method for producing adenosine by fermentation | |
| Haider | Bioconversion of saw dust powder acid hydrolysis to single cell protein by the yeast Candida tropicalis | |
| CN112430636B (en) | Method for producing ademetionine by biological method | |
| CN101870964B (en) | Method for improving SAM synthetase expression level | |
| CN112592945A (en) | Adenosine fermentation process | |
| CN110468051B (en) | K252A fermentation medium and preparation method thereof | |
| CN111073922B (en) | Vitamin B 12 Fermentation supplementing medium and supplementing method | |
| CN114134056A (en) | Saccharomyces cerevisiae ZJS10041 and application thereof in fermentation production of S-adenosylmethionine | |
| CN113604390A (en) | Corynebacterium glutamicum and application thereof in fermentation production of L-ornithine | |
| CN114276937A (en) | Method for fermenting paecilomyces hepiali by using Chinese yam as carbon source | |
| CN114015607A (en) | Bacillus amyloliquefaciens for high yield of 5-methyltetrahydrofolic acid and application thereof | |
| CN102533604A (en) | Brevibacterium flavum and application thereof as well as method for preparing lysine through fermentation | |
| CN110408667A (en) | A kind of zymotechnique improving beta-thymidine yield |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |