CN112545912A

CN112545912A - Novel application of L-fucose

Info

Publication number: CN112545912A
Application number: CN201910858728.0A
Authority: CN
Inventors: 朱华伟; 李泓颖; 罗珍; 余庆涛; 唐健
Original assignee: Infinitus China Co Ltd
Current assignee: Infinitus China Co Ltd
Priority date: 2019-09-11
Filing date: 2019-09-11
Publication date: 2021-03-26

Abstract

The invention relates to the field of medicines, relates to a new application of L-fucose, and particularly relates to an application of L-fucose in preparation of products with alopecia prevention and hair growth promotion effects. In-vitro experiments show that the L-fucose can remarkably up-regulate the expression of beta-catenin genes, IGF-1 genes, KGF-2 genes and VEGF genes (p is less than 0.001), promote the expression of MMP-2 genes and MMP-9 genes (p is less than 0.001), help to induce or maintain the growth phase of hair follicles, and promote the growth of the hair follicles and the remodeling of dermal papilla extracellular matrix; meanwhile, the expression of an anti-apoptosis gene Bcl-2 is obviously up-regulated (p <0.001), and the ratio of Bax/Bcl-2 is down-regulated (p <0.005), so that the apoptosis of dermal papilla cells is inhibited. The L-fucose has good effects of preventing alopecia and nourishing hair.

Description

Novel application of L-fucose

Technical Field

The invention relates to the field of medicines, in particular to novel application of L-fucose, and especially relates to application of L-fucose or a polymer thereof in preparation of products with effects of preventing alopecia and nourishing hair.

Background

The hair is a natural high molecular keratin fiber and can be divided into four parts, namely a hair papilla, a hair follicle, a hair root and a hair shaft from bottom to top. It is chemically inert but sensitive to light, boiling water, acids, bases, oxidizing and reducing agents. Frequent scalding and dyeing, improper stretching, bad weather, staying up all night, irregular diet, excessive mental stress and the like in daily life can cause damage to hair and even fall off. The physiological characteristics and functions of hair depend mainly on the papilla, hair follicle, sebaceous gland, etc. below the scalp epidermis.

The hair follicles are continuously alternated in cycles of various phases of proliferation (anagen), degeneration (catagen) and rest (telogen) with a continuous growth of new hair. The papilla is located at the base of the follicular bulb, is the most important dermal component of the follicle, and is composed of important structures such as papilla cells, extracellular matrix, and vascular nerves. It not only plays an important regulatory role in the follicular cycle, but also has maintenance and induction effects. The structure and composition of the hair papilla vary with the hair follicle cycle as observed by the Down founder et al in vitro culture model of hair follicles. During the growth period, the hair papilla is embedded in a loose connective tissue matrix, surrounded by the epithelial cells of the hair bulb, and separated from the epithelial cells through three layers of complete basement membranes; when the hair follicle begins to grow, the volume of the hair papilla is increased, the hair papilla becomes slender and has a loose structure, and the hair matrix structures are in close contact; when the hair follicle growth is stopped or in a resting stage, the extracellular matrix of hair papilla cells is reduced, epithelial cells in the hair bulb part recede and separate from the hair papilla, and the hair papilla shrinks and stays at the proximal end of the base part of the hair follicle to form a compact bundle of cells, which is embedded in the extracellular matrix.

Hair papilla cells (DPC) are a group of cells of dermal origin located at the base of the hair follicle, and play a major regulatory role in the periodic circulation of the hair follicle and hair growth. Hair papillae consists of a population of very distinct fibroblasts that produce distinct ECM's that are important in epithelial-mesenchymal interactions. DPC is a specific mesenchymal component, the most prominent cell that regulates hair follicle development and periodic reconstruction. It has been shown that the β -catenin molecule in the Wnt signaling pathway is the major signaling molecule for maintaining DPC-induced activity and hair papilla cell growth.

The transformation of the various stages of the hair follicle growth cycle is controlled by the interaction of hair follicle keratinocytes (hair follicle epithelial cells) and dermal papilla fibroblasts between epithelial and mesenchymal tissue. The dermal papilla is essential for inducing and maintaining the periodic circulation of the hair follicle, and its size (determined by the number of stromal cells in the hair bulb portion) determines the size of the hair bulb in the anagen phase, the length of the anagen phase, and the length and diameter of the hair shaft. If the papilla is absent, or if the papilla is not in intimate contact with the hair matrix keratinocytes above it, the growth phase will be difficult to maintain. It is known that hair papilla cells secrete various growth factors and cytokines, such as insulin-like growth factor (IGF) -1 (maintaining and promoting hair growth), Fibroblast Growth Factor (FGF) -7, FGF-5S (lacking in the growth stage of long hair follicles), Hepatocyte Growth Factor (HGF) (stimulating cellular DNA synthesis, promoting the growth of various epithelial cells and melanocytes, lengthening hair shafts), Vascular Endothelial Growth Factor (VEGF) (promoting the proliferation and migration of hair follicle cells), and the like, to induce proliferation of cells of stroma, dermal papilla vascular system, increase the amount of dermal papilla extracellular matrix, and maintain hair follicles in the growth stage.

Hair growth relies on the vascular network of the papilla to supply nutrients. The mRNA expression of Vascular Endothelial Growth Factor (VEGF) varies in different growth cycles of hair: VEGF mRNA is strongly expressed in papillary cells during the growth phase and is less expressed during the catagen and telogen phases. The oral administration product can better promote the vascularization of dermal papilla (such as promoting the expression of VEGF), increase the local blood supply and provide more nutrition and oxygen for the growth of hair follicles.

At present, a plurality of products for treating and preventing alopecia are available, and comprise western medicines and traditional Chinese medicines. The western medicine products mainly comprise oral finasteride and external minoxidil, both of which are hair restorers approved by FDA to be on the market, and have obvious effects, but higher price and obvious side effects, for example, finasteride easily causes male dysfunction, minoxidil easily causes dermatitis and allergy, and drug resistance is easily generated after long-term use; most of the traditional Chinese medicine products have complex compositions and slow effect, and lack traditional Chinese medicine products which can really solve the problem of alopecia.

L-fucose, i.e. 6-deoxy-L-galactose, also known as ancylose, is a compound of formula C₆H₁₂O₅The deoxy six-carbon sugar of (1). L-fucose exists on the surface of mammalian and plant cells, and can be added into various cosmetics, medicines and dietary supplements. Its optical isomer D-fucose belongs to a rare sugar in nature, and appears only in some glycosides, polysaccharides. The structural formulas of the L-fucose and the D-fucose are as follows:

the fucose monomers may be polymerized to form fucoidan, and the alpha-fucosidase may be hydrolyzed from the fucose-containing polymer to produce fucose. Seaweed is rich in fucose and exists in the form of sulfated fucose polymers, i.e., fucoidan. The brown algae contains abundant fucoidin, and the saccharide configuration of the heteropolysaccharide is analyzed, so that the content of L-fucose in the heteropolysaccharide can reach more than 40%.

The L-fucose has low sweetness and low calorific value, has a series of physiological functions beneficial to human body, and can meet the requirements of human body on nutrition and health. It is convenient to use, has excellent acid resistance and heat resistance, and can be widely applied to the fields of food and cosmetics. L-fucose has been reported to regulate the balance of intestinal flora, reduce the content of triglyceride and cholesterol in serum, and improve immunity. At present, no relevant reports of L-fucose on the aspects of alopecia prevention and hair growth are found in the prior art.

Disclosure of Invention

In view of the above, the present invention aims to provide a novel application of L-fucose or its polymer, and particularly an application of L-fucose or its polymer in the preparation of products with effects of preventing hair loss and nourishing hair.

In-vitro experiments show that the L-fucose can remarkably up-regulate the expression of beta-catenin genes, IGF-1 genes, KGF-2 genes and VEGF genes (p is less than 0.001), promote the expression of MMP-2 genes and MMP-9 genes (p is less than 0.001), help to induce or maintain the growth phase of hair follicles, and promote the growth of the hair follicles and the remodeling of dermal papilla extracellular matrix; meanwhile, the expression of an anti-apoptosis gene Bcl-2 is obviously up-regulated (p <0.001), and the ratio of Bax/Bcl-2 is down-regulated (p <0.005), so that the apoptosis of dermal papilla cells is inhibited. The results show that the L-fucose can obviously promote the growth of hair follicles, stimulate the growth of dermal papilla, inhibit the apoptosis of dermal papilla cells and promote the remodeling of dermal papilla extracellular matrix, and has good effects of preventing alopecia and nourishing hair. D-fucose has no such effect.

In the present invention, the L-fucose is derived from chemically synthesized or natural sources, wherein the natural sources include, but are not limited to, fucoidan, algal plants, and the like. D-fucose belongs to rare sugar, is only found in some glycoside compounds in nature, and is synthesized by adopting a chemical modification method.

The invention also provides application of the L-fucose polymer in preparing products with the functions of preventing alopecia and nourishing hair.

The weight average molecular weight (Mw) of the fucoidin is approximately 200000, wherein the content of the L-fucose monomer is 20% -40%.

The invention provides an application of L-fucose or a polymer thereof in preparing a product with the effects of preventing alopecia and nourishing hair, wherein the effects of preventing alopecia and nourishing hair at least comprise one of the following effects:

promoting hair follicle growth, stimulating dermal papilla growth, inhibiting apoptosis of dermal papilla cells, and promoting dermal papilla extracellular matrix remodeling.

In some embodiments, the promoting hair follicle growth is specifically promoting expression of at least one gene selected from the group consisting of a β -catenin gene, an MMP-2 gene, an MMP-9 gene, and a growth factor gene.

In some embodiments, the growth factor is at least one of insulin growth factor 1, keratinocyte growth factor 2, and vascular endothelial growth factor.

In some embodiments, the promotion of dermal papilla extracellular matrix remodeling is specifically the promotion of expression of an MMP-2 gene and/or an MMP-9 gene.

In some embodiments, the inhibition of dermal papilla cell apoptosis is specifically promoting expression of anti-apoptotic gene Bcl-2, down-regulating Bax/Bcl-2 ratio.

In some embodiments, the product is a hair cosmetic, food, or health food.

Specifically, the hair cosmetics include hair cosmetics, hair care cosmetics, and hair cosmetics.

The food or health food is capsule, tablet, powder, granule, pill, beverage or teabag.

In the application of the L-fucose in preparing products with the effects of preventing alopecia and nourishing hair, the effective dose of the L-fucose or the polymer thereof is 100-⁵Individual cell).

In some embodiments, the effective dose of L-fucose is 200. mu.g/(1X 10)⁵Individual cell).

In some embodiments, L-fucose is administered at a concentration of 25-200 μ g/ml. In some embodiments, L-fucose is administered at a concentration of 25. mu.g/ml, 50. mu.g/ml, 100. mu.g/ml, or 200. mu.g/ml.

The invention provides a new application of L-fucose, in particular to an application of L-fucose or a polymer thereof in preparing products with the effects of alopecia and hair nourishing. In vitro experiments show that L-fucose can remarkably up-regulate the expression of beta-catenin gene, IGF-1 gene, KGF-2 gene and VEGF gene (p is less than 0.001), promote the expression of MMP-2 and MMP-9 (p is less than 0.001), and is helpful for inducing or maintaining the growth phase of hair follicles; remarkably up-regulating the expression of an anti-apoptosis gene Bcl-2 (p <0.001) and down-regulating the ratio of a Bax/Bcl-2 gene (p <0.005), thereby inhibiting the apoptosis of dermal papilla cells; the L-fucose can obviously promote the growth of hair follicles, stimulate the growth of dermal papilla, inhibit the apoptosis of dermal papilla cells and promote the remodeling of dermal papilla extracellular matrix, and has good effects of preventing alopecia and nourishing hair.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.

FIG. 1 shows the effect of L-fucose, D-fucose on the extension degree of hair shafts of hair follicles cultured in vitro;

FIG. 2 shows the effect of L-fucose and D-fucose on growth of dermal papilla cells;

FIG. 3 shows the effect of L-fucose on the apoptosis of papillary cells, wherein 3-A is the expression of the anti-apoptotic factor Bcl-2 and 3-B is the ratio Bax/Bcl-2;

FIG. 4 shows the effect of L-fucose on extracellular matrix remodeling, wherein 4-A is MMP2 expression, 4-B is MMP9 gene expression, and D-fucose has no effect on MMPs expression;

FIG. 5 shows the effect of L-fucose on the expression of growth factors, wherein 5-A to 5-D are the expression of β -catenin (5-A), IGF-1(5-B), KGF-2(5-C) and VEGF (5-D) in this order.

Detailed Description

The invention discloses application of L-fucose or a polymer thereof in preparing products with the effects of preventing alopecia and nourishing hair, and a person skilled in the art can use the contents in the text for reference and appropriately improve process parameters to realize the purpose. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

The invention is further illustrated by the following examples:

example 1 Effect of L-fucose on the extension degree of Hair shafts of Hair follicles cultured in vitro

The hair follicle is obtained from a patient who is subjected to hair transplantation operation in dermatology department of Huashan Hospital affiliated to the university of Compound Dan, and is obtained after the consent of the patient.

Hair follicles were isolated and incubated: hair follicles in the anagen phase were selected, one third cut from the top and the remaining two thirds placed in Williams E medium (Sigma). The culture medium was supplemented with L-fucose or D-fucose at a concentration of 200. mu.g/ml, and the normal groups were placed in the medium without treatment, and all groups were cultured in a 5% CO2 incubator (Thermo 371) at 37 ℃ for 3 days and 6 days, and the medium was changed every 2 days. In vitro hair follicles in culture were photographed at 3 and 6 days, and hair shaft lengths were measured and recorded. The results are shown in FIG. 1.

As can be seen from FIG. 1, the net growth rates of hair shafts in the L-fucose-treated group were (10.49. + -. 3.19)% and (13.55. + -. 3.60)%, respectively, after 3 days and 6 days of co-culture, and were significantly different from those in the normal group (p <0.05), and the net growth rates of hair shafts in the D-fucose-treated group were (3.74. + -. 1.82)% and (2.72. + -. 1.42)%, respectively, and were not significantly different from those in the normal group.

Example 2: effect of L-fucose on growth of dermal papilla cells

Dermal papillae were dissected from the follicular bulb using a surgical microscope and then transferred to a petri dish containing DMEM medium (Sigma). The medium contains 50-100U/ml penicillin and 50-100. mu.g/ml streptomycin and 20% heat-inactivated fetal bovine serum (Hyclone). Directly digesting the dermal sheath tissue of the lower part of the hair follicle by adopting a collagenase D digestion method to obtain hair papilla cells. The culture dish was placed in a 5% CO2 incubator at 7 ℃ and the solution was changed every 2 days, and after 3 to 4 days of culture, the cells were passaged until the confluency became 90%. The cells were removed from the incubator, observed by inverted microscope for cell morphology and recorded. The medium was aspirated from the dish using a pipette and washed 2 times with DPBS. Adding 0.25% Tripsin-EDTA for digestion, placing the cells in an incubator at 37 deg.C until 80-90% of cells are rounded and detached, adding a hair papilla cell growth medium to terminate the reaction, and collecting in a 50ml centrifuge tube. Centrifuge at 1800rpm for 5 min. The supernatant was discarded and the cells were resuspended in medium. The centrifugation resuspension step was repeated twice, eventually counted in Trypan Blue, and the plates were seeded with a cell density of 5-8X 10^ 5. After inoculation, the flasks were placed in a 5% CO2 incubator at 37 ℃ and the medium was changed every 2 days, and after 3-4 days of culture, the cells were passaged until the confluency was 90%. Finally, P3 generation cells were selected for the experiment.

Dermal papilla cells were seeded at a cell density of 5000 cells/well in 96-well plates and cultured at 37 ℃ in a 5% CO2 incubator to 90% confluency for experiments. Testosterone with the concentration of 30 mu g/ml is added into a cell culture medium of a model group to simulate the adverse environment of testosterone affecting hair papilla cells in androgen alopecia, while L-fucose or D-fucose with the concentrations of 25 mu g/ml, 50 mu g/ml, 100 mu g/ml and 200 mu g/ml is added into the culture medium while testosterone is added into other groups, only the culture medium is added into a normal group, after 3 days of incubation, the culture medium is discarded, the cells are washed for 1-2 times by PBS, and the cell viability is detected by using an MTT method. The results are shown in FIG. 2.

The results show that when the L-fucose concentrations of 25 mug/ml, 50 mug/ml, 100 mug/ml and 200 mug/ml are used, no obvious stimulation effect is caused on the growth of hair papilla cells, and the L-fucose has no toxic and side effects. The number of hair papilla cells remains stable during the hair follicle growth cycle, but the extracellular matrix of the hair papilla is significantly altered. When the D-fucose and the hair papilla cells are co-cultured, no obvious toxic or side effect is caused on the growth of the hair papilla cells.

Example 3 Effect of L-fucose on the Gene expression levels of β -catenin, Bcl-2, Bax, MMP-2, MMP-9, VEGF, IGF-1, KGF-2

The first test method comprises the following steps: dermal papilla cells were cultured in a medium containing 100. mu.g/ml of L-fucose or D-fucose, only the medium was added to the normal group, testosterone was added to the medium of the model group at a concentration of 30. mu.g/ml, incubation was carried out for 3 days, and then RNA was extracted, reverse transcription and Real-time PCR analysis were carried out.

TABLE 1 reagents (kit) for RNA extraction, reverse transcription PCR, Real-time PCR

TABLE 2 Main instruments for RNA extraction, reverse transcription, Real-time PCR

The RNA extraction process avoids the degradation of RNA, the operation is carried out on ice, and the operation is as rapid as possible. In the experiment, RNeasy Mini Kit was used to extract total RNA. The reverse transcription reaction was performed using the Rever Transace-. alpha.Transcriptase Kit to obtain cDNA. Selection primers were designed using PrimerQuest website software as follows:

TABLE 3Real-time PCR primer sequences

Target baseDue to the fact that	Primer sequences
		β-catenin-Forward	5′-CCGTTCGCCTTCATTATGGA-3′
β-catenin-Reverse	5′-GGCAAGGTTTCGAATCAATCC-3′
		Bcl-2-Forward	5′-ATCGCCCTGTGGATGACTGAG-3′
Bcl-2-Reverse	5′-CAGCCAGGAGAAATCAAACAGAGG-3′
		Bax-Forward	5′-GGACGAACTGGACAGTAACATGG-3′
Bax-Reverse	5′-GCAAAGTAGAAAAGGGCGACAAC-3′
		MMP-2-Forward	5′-CCGTCGCCCATCATCAA-3′
MMP-2-Reverse	5′-AGATATTGCACTGCCAACTCT-3′
		MMP-9-Forward	5′-TCGTGGTTCCAACTCGGTTT-3′
MMP-9--Reverse	5′-GCGGCCCTCGAAGATGA-3′
		VEGF-Forward	5′-TTTGGAGCAGCCACAAACACCTTC-3′
VEGF-Reverse	5′-TGAGGTAACCTGTGCTGGTGTTCA-3′
		IGF-1-Forward	5′-TCGCATCTCTTCTATCTGGCCCTGT-3′
IGF-1-Reverse	5′-GCAGTACATCTCCAGCCTCCTCAGA-3′
		KGF-2-Forward	5′-ACATTGTGCCTCAGCCTTTC-3′
KGF-2-Reverse	5′-ACCATGTCCTGACCAAGAGC-3′
		GAPDH-Forward	5′-ACAAGATGGTGAAGGTCGGTGTGA-3′
GAPDH-Reverse	5′-AGCTTCCCATTCTCAGCCTTGACT-3′

The above primers were synthesized by Shanghai Biotech engineering Co.

The invention analyzes the expression level of beta-catenin, Bcl-2, Bax, MMP-2, MMP-9, VEGF, IGF-1 and KGF-2 genes. The experiments were performed in 384-well plates and cDNA quantification was performed using the Real-time PCR method (SYBR Green method). The DNA double-strand non-specific embedded dye SYBRGreen can be specifically bound to double-strand DNA but not single-strand DNA, the luminous intensity of the bound dye can be obviously increased, and the dye which is not bound can only emit very weak fluorescence in a solution. 7900HT Fast Real-Time PCR system was used in the experiments.

Secondly, statistical analysis: statistical deviation analysis was performed using the t-test. The probability (P) <0.05 was considered statistically significant for this experimental result. And (3) carrying out relative quantification on the expression quantity of the target gene by adopting a comparative CT value method according to experimental data of Real-time PCR. Relative quantification refers to the comparison of two or more genes with each other, resulting in a ratio. GAPDH was selected as a reference in the experiment.

Thirdly, analyzing results:

1. inhibition of dermal papilla apoptosis by L-fucose.

Androgen stimulation induces apoptosis of dermal papilla cells of hair follicles, L-fucose can remarkably up-regulate the expression of an anti-apoptosis gene Bcl-2 and down-regulate the proportion of a Bax/Bcl-2 gene, and prevent rapid death of the dermal papilla cells. However, D-fucose did not show an effect of preventing/improving apoptosis of hair papilla cells in the case of testosterone stimulation (as shown in fig. 3). Effect of 2, L-fucose on remodeling of dermal papilla extracellular matrix in vitro

2. Promotion of remodeling of dermal papilla extracellular matrix (ECM) by L-fucose

During ECM remodeling, MMPs release growth factors from the ECM, stimulating cell migration, and are critical for long-term maintenance. As can be seen from FIG. 4, L-fucose promotes the expression of MMP-2 and MMP-9 genes, two most important members of the MMP family, and promotes the maintenance of the hair follicle growth cycle, while D-fucose does not.

3. Effect of L-fucose on growth factor expression in dermal papilla cells

L-fucose can significantly up-regulate gene expression of beta-catenin (shown in figure 5-A), IGF-1 (shown in figure 5-B), KGF-2 (shown in figure 5-C) and VEGF (shown in figure 5-D), and is helpful for inducing or maintaining the anagen phase of the hair follicle and realizing self-reconstruction of the hair follicle. D-fucose failed to significantly alter the expression of each signal molecule or growth factor, with no effect on the hair follicle growth cycle, especially anagen phase.

The results show that the L-fucose can remarkably up-regulate the expression of beta-catenin gene, IGF-1 gene, KGF-2 gene and VEGF gene (p is less than 0.001), promote the expression of MMP-2 gene and MMP-9 gene (p is less than 0.001), help to induce or maintain the growth phase of hair follicles, and promote the growth of hair follicles and the remodeling of dermal papilla extracellular matrix; meanwhile, the expression of an anti-apoptosis gene Bcl-2 is obviously up-regulated, and the ratio of Bax/Bcl-2 (p <0.001) is down-regulated, so that the apoptosis of dermal papilla cells is inhibited. The L-fucose can obviously promote the growth of hair follicles, stimulate the growth of dermal papilla, inhibit the apoptosis of dermal papilla cells and promote the remodeling of dermal papilla extracellular matrix, thereby having good effects of preventing alopecia and nourishing hair. D-fucose has no such effect.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

Application of L-fucose or a polymer thereof in preparing a product with effects of preventing alopecia and nourishing hair.
2. The use according to claim 1, wherein the effects of preventing hair loss and growing hair comprise at least one of the following:

promoting hair follicle growth, stimulating dermal papilla growth, inhibiting apoptosis of dermal papilla cells, and promoting dermal papilla extracellular matrix remodeling.
3. Use according to claim 2, wherein the promotion of hair follicle growth is in particular the promotion of expression of at least one gene selected from the group consisting of the β -catenin gene, the MMP-2 gene, the MMP-9 gene and the growth factor gene.
4. The use of claim 3, wherein the growth factor is at least one of insulin growth factor 1, keratinocyte growth factor 2, and vascular endothelial growth factor.
5. Use according to claim 2, characterized in that said promotion of dermal papilla extracellular matrix remodelling is in particular the promotion of the expression of the MMP-2 gene and/or of the MMP-9 gene.
6. Use according to claim 2, characterized in that said inhibition of apoptosis of dermal papilla cells is in particular a promotion of the expression of the anti-apoptotic gene Bcl-2, a down-regulation of the Bax/Bcl-2 ratio.
7. Use according to claim 1, characterized in that the product is a hair cosmetic, a food product or a health food.
8. Use according to claim 7, characterized in that the hair cosmetics comprise hair cosmetics, hair care cosmetics and hair cosmetics.
9. The use according to claim 7, wherein the food or health food is a capsule, tablet, powder, granule, pill, beverage or teabag.
10. The use according to any one of claims 1 to 9, wherein the effective amount of the L-fucose or the polymer thereof is 100-⁵Individual cell).