CN112544989A - A composition with muscle protecting effect - Google Patents
A composition with muscle protecting effect Download PDFInfo
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- CN112544989A CN112544989A CN202011511533.8A CN202011511533A CN112544989A CN 112544989 A CN112544989 A CN 112544989A CN 202011511533 A CN202011511533 A CN 202011511533A CN 112544989 A CN112544989 A CN 112544989A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
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- A—HUMAN NECESSITIES
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Abstract
The invention discloses a composition with a muscle protection effect, which comprises the following raw materials in parts by weight: 11.13-100.09 parts of fermented soybean protein peptide powder, 10.99-98.83 parts of whey protein, 5.08-45.68 parts of prebiotics and 0.31-2.81 parts of mushroom powder, wherein the purity of the mushroom powder is 10%. The invention uses the fermented soybean protein peptide powder with low allergenicity, and the mushroom powder has obvious synergistic protection effect on human muscle when being combined with the fermented soybean protein, the whey protein and the prebiotics substances. The raw materials in the formula are common food raw materials, so that the food is suitable for all people needing protein supplementation, and large-scale industrial production can be realized.
Description
Technical Field
The invention discloses a food composition with a muscle protection effect, and belongs to the technical field of food processing.
Background
With increasing age and decreasing energy demand, food and energy intake drops by an average of 25% from age 40 to age 70. Low food intake and a monotonous diet put the elderly at risk because as the total food intake decreases, the intake of most nutrients also decreases accordingly. In a consolidated analysis of data from 12 countries, approximately two thirds of the elderly participating in the study were determined to have nutritional problems or malnutrition.
In 2018, the European osteoporosis, osteoarthritis and musculoskeletal disease Clinical and economic problems Association (ESCEO) working group multiple experts published an important review article in the journal of Clinical Nutrition entitled "Does Nutrition play a role in the prevention and treatment of sarcopenia" (ROBINSON S M, REGISTER J Y, RIZZOLI R, et al. Does Nutrition plan a role in the prevention and management of sarcopenia [ J ]. Clinical Nutrition, 2018, 37: 1121 1132.). The importance of a "healthier" dietary pattern, which is the need to ingest sufficient protein, vitamin D, antioxidant nutrients and long chain polyunsaturated fatty acids, is pointed out in this article, with a large body of evidence supporting dietary proteins and physical activity as key synthetic stimulators of muscle protein synthesis.
Verreijen et al (VERREIJEN A M, VERLAAN S, ENGBEINK MF, et al. A high blood protein-, leucin-, and vitamin D-expressed present muscles bone protein expression in vivo users adducts: a double-blind regulated controlled three [ J ]. Am J Clin Nutr, 2015, 101: 279 286.) in a double-blind random control trial with a 13 week controlled diet plus exercise for obese elderly (BMI > 30) aged more than 55 years, each of the supplementation consisting of 20g of lactalbumin, 2.8 g of leucine and 1 part of vitamin D after 3 resistive exercises before and after 3 meals a day, the results showed that the high leucine and D protein supplements maintained low muscle mass at SMI and muscle resistance including muscle mass in elderly people (SMI) compared to isocaloric control groups, Muscle strength and function) and thus may reduce the risk of sarcopenia.
Patent CN103766696B discloses a composition with muscle-increasing function, which contains the following components: 30-60 parts of whey protein, 10-30 parts of wheat protein, 5-20 parts of soybean oligopeptide, 1-5 parts of leucine, 60.1-1 parts of vitamin B, 0.001-0.005 part of vitamin D and 0.35-0.8 part of calcium. The composition patent is shown to facilitate protein absorption and utilization by the elderly, promote muscle synthesis, and reduce age-related muscle attenuation. It is known that leucine as a functional food ingredient cannot be applied to ordinary foods, and the muscle-building effect is greatly impaired due to the lack of the support of leucine in the above formula. Meanwhile, the addition amount of the vitamin D is too high, the intake amount of the vitamin D is 10 mug/day according to the diet recommendation of Chinese residents, and the tolerable maximum intake amount is 50 mug/day. According to the patent formula, the vitamin D intake of 20g of the product taken every day is in the range of 200 mug-1000 mug, and far exceeds the tolerable highest intake, excessive vitamin D intake for a long time can cause hypercalcemia, hypercalciuria and the like, the poisoning risk can be increased, and death can be caused due to serious vitamin D poisoning. In addition, the wheat protein contains allergenic components, which are not conducive to digestion and absorption by the human body.
Therefore, it is necessary to search a common health food with obvious muscle protection effect, which is more beneficial for the masses of people to eat at ease.
Disclosure of Invention
Based on the defects of the prior art, the invention discloses a common food which does not contain allergenic components and has obvious muscle protection effect.
In order to achieve the purpose, the invention adopts the technical scheme that:
a composition with muscle protection effect comprises the following raw materials in parts by weight: 11.13-100.09 parts of fermented soybean protein peptide powder, 10.99-98.83 parts of whey protein, 5.08-45.68 parts of prebiotics and 0.31-2.81 parts of mushroom powder, wherein the purity of the mushroom powder is 10%.
As a preferable scheme, the composition comprises the following raw materials in parts by weight: 50.05-100.09 parts of fermented soybean protein peptide powder, 49.42-98.83 parts of whey protein, 22.84-45.68 parts of prebiotics and 1.4-2.81 parts of mushroom powder, wherein the purity of the mushroom powder is 10%.
As a preferred scheme, the fermented soybean protein powder is soybean protein powder with protein content of more than or equal to 80 percent and peptide content of more than or equal to 18 percent.
As a more preferable scheme, the fermented soybean protein powder is prepared by taking soybeans as raw materials, and adopting enzymolysis and fermentation of lactobacillus paracasei and pediococcus pentosaceus composite strains.
Preferably, the prebiotics are inulin, fructo-oligosaccharide or xylo-oligosaccharide.
According to the composition, fermented soybean protein powder and mushroom powder are introduced, the fermented soybean protein powder is rich in multiple nutritional active ingredients such as whole protein, polypeptide, oligopeptide, organic acid and the like through the processes of fermentation and enzymolysis, and the effect of protein supplementation by gradient absorption of multiple nitrogen sources formed by large-molecule whole protein, polypeptide, oligopeptide and amino acid is good; in addition, by combining the enzymolysis composite fermentation technology, the conformational epitope and the linear epitope of the raw material protein allergen can be destroyed, the allergic signal path is weakened, the sensitization risk is effectively reduced, and the zebra fish anaphylactic reaction test proves that the fermented soybean protein peptide powder can not induce anaphylactoid reaction. Meanwhile, mushroom powder raw materials are creatively introduced, and since the mushrooms contain rich lysine which is essential amino acid for human bodies, the mushroom powder can increase the height and the weight and can enhance the disease resistance. Therefore, when the mushroom powder is combined with the fermented soy protein, the lactalbumin and the prebiotics substance, the mushroom powder has obvious synergistic protection effect on the muscle of the human body. In addition, prebiotic nutritional ingredients such as inulin, fructo-oligosaccharide, xylo-oligosaccharide and the like are introduced, so that the negative influence on intestinal microbiota possibly caused by long-term protein supplement is avoided. The raw materials in the formula are common food raw materials, and the food is suitable for all people needing protein supplementation, so that large-scale industrial production can be realized.
Drawings
FIG. 1 is a pathological section of zebrafish test of the compositions prepared in examples 1-3 and comparative examples 1-5 of the present invention.
Detailed Description
Unless otherwise specified, the raw materials used in the examples of the present invention were purchased from the market, the fermented soybean protein peptide powder of the present invention was purchased from biotechnology limited, zhejiang hai, and the fermented soybean protein peptide powder was prepared from isolated soybean protein as a raw material by enzymolysis and fermentation, and the protein content was not less than 80%, and the peptide content was not less than 18%. The mushroom powder is made by self and has the purity of 10 percent.
For better illustrating the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to specific examples, which are intended to be understood in detail, but not intended to limit the present invention.
The inventive examples 1 to 3 and comparative examples 1 to 5 were weighed according to table 1 and mixed uniformly for use.
Test examples
First, test animal
The zebra fish are all bred in fish breeding water at 28 ℃ (water quality: 200 mg of instant sea salt is added into per 1L of reverse osmosis water, the conductivity is 450-550 muS/cm, the pH is 6.5-8.5, the hardness is 50-100 mg/L CaCO 3), and the license number of experimental animals is as follows: SYXK (Zhe) 2012-0171. The feeding management meets the requirements of the international AAALAC certification (certification number: 001458).
Wild type AB line zebrafish, bred by natural pairwise mating, aged 2 days after fertilization (2 dpf).
Second, instrument, consumable and reagent
2.1 instruments
Biomicroscopes (CX 31, OLYMPUS, Japan); dissecting microscopes (SZX 7, OLYMPUS, Japan); precision electronic balances (CP 214, OHAUS, USA); 6-well plates (Nest Biotech, China).
Second, instrument, consumable and reagent
4% tissue cell fixative (batch No. 20190923, Solarbio); xylene (batch No. C10631736, shanghai mclin biochem technologies ltd.); PBS buffer (batch No. 10C16B30, Wuhan doctor De bioengineering Co., Ltd.); mayer hematoxylin staining solution (batch No. YH0170326, shanghai ihh biotechnology limited); eosin staining solution (batch No. YH180124, ihh biotechnology ltd, shanghai); neutral resin coated tablets (batch No. YH0170419, ihhe biotechnology limited, shanghai); soft wax (melting point 54-56 ℃, batch number 20180108, Shanghai Huayong Paraffin Co., Ltd.); hard wax (melting point 62-64 ℃, batch number 20190628, Shanghai Huayong Paraffin Co., Ltd.); absolute ethanol (batch No. 20200104, national pharmaceutical group chemical Co., Ltd., China).
Third, test method
3.1. Experimental groups
3.2. Test method
Evaluation of sample muscle protection efficacy (pathological section)
240 tail 2 dpf wild type AB strain zebra fish were randomly selected in six-well plates, and 30 tail zebra fish were treated per well (experimental group). Except for a normal control group, all the other experimental groups are dissolved in water and are given 1.5 percent of absolute ethyl alcohol for 24 hours to establish a zebra fish alcoholic muscle injury model. Samples were separately dosed in water at the concentrations indicated in the above table, while a normal control group and a model control group were set at a volume of 3 mL per well. After 24H treatment at 28 ℃, zebrafish were fixed with 4% tissue cell fixative, H & E stained (fix-dehydrate-embed-slice-stain) and pathologically analyzed for sample muscle protection efficacy. The samples were scored for muscle protection efficacy according to table 2.
Remarking: normal score 0, mild muscle damage score 1-4, moderate muscle damage score 5-6, and severe muscle damage score 7-8.
Fourth, test results
The length, infiltration degree and necrosis degree of the muscles of all groups are 0. The results of the summary of the clarity and the relaxation are shown in Table 3, and the pathological section is shown in FIG. 1.
Test remarks:
& is relative to the mock control group, # is relative to the equivalent concentration protein + inulin group (example 1 group versus the comparative example 2 group, example 2 group versus the comparative example 4 group) # is relative to the comparative example 5.
2. Three identical symbols indicate p < 0.001, two identical symbols indicate p < 0.01, and are extremely significant, and one identical symbol indicates p < 0.05, and significantly, the smaller the p value, the more significant the difference.
3.0 for normal, 1-4 for mild muscle damage, 5-6 for moderate muscle damage, and 7-8 for severe muscle damage.
As can be seen from Table 4:
the model control group has extremely significant difference compared with the normal control group, which indicates that the molding is successful. There was no significant difference between the comparative example 1 group and the comparative example 3 group relative to the model control group, indicating that mushroom powder in this concentration range had no muscle-protecting efficacy. The comparative example 2 group and the comparative example 4 group have significant difference relative to the model control group, which shows that the protein + inulin group has protective effect on the muscle, and the severe muscle damage of the model group is reduced to moderate damage. The groups 1-3 had very significant differences compared to the model control group, indicating that the test group had significant muscle protection.
The groups of example 1 and example 2 have significant differences relative to the groups of protein and inulin (comparative example 2 and comparative example 4) at the same concentration, which shows that the mushroom powder groups (comparative example 1 and comparative example 2) and the groups of protein and inulin (comparative example 2 and comparative example 4) have synergistic effects, severe muscle damage in the model group can be reduced to mild damage, and the muscle protection effect is obvious. Example 3 has a very significant difference with respect to comparative example 5 (same total concentration), indicating that the experimental formulation has a better muscle protection efficacy than comparative example 5.
Claims (5)
1. The composition with the muscle protection effect is characterized by comprising the following raw materials in parts by weight: 11.13-100.09 parts of fermented soybean protein peptide powder, 10.99-98.83 parts of whey protein, 5.08-45.68 parts of prebiotics and 0.31-2.81 parts of mushroom powder, wherein the purity of the mushroom powder is 10%.
2. The composition according to claim 1, comprising the following raw materials in parts by weight: 50.05-100.09 parts of fermented soybean protein peptide powder, 49.42-98.83 parts of whey protein, 22.84-45.68 parts of prebiotics and 1.4-2.81 parts of mushroom powder, wherein the purity of the mushroom powder is 10%.
3. The composition according to claim 1 or 2, characterized in that: the fermented soybean protein powder is soybean protein powder with protein content more than or equal to 80% and peptide content more than or equal to 18%.
4. The composition according to claim 1 or 2, characterized in that: the fermented soybean protein powder is prepared by taking soybeans as raw materials, and adopting directional enzymolysis and fermentation of lactobacillus paracasei and pediococcus pentosaceus composite strains.
5. The composition according to claim 1 or 2, characterized in that: the prebiotics are inulin, fructo-oligosaccharide or xylo-oligosaccharide.
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