CN112538413A

CN112538413A - Biological reaction device and biological reaction method

Info

Publication number: CN112538413A
Application number: CN202011280106.3A
Authority: CN
Inventors: 李庭刚; 徐求; 朱加雷; 韦漩
Original assignee: Institute of Process Engineering of CAS
Current assignee: Institute of Process Engineering of CAS
Priority date: 2020-11-16
Filing date: 2020-11-16
Publication date: 2021-03-23

Abstract

The invention provides a biological reaction device and a biological reaction method, wherein the biological reaction device comprises a shell, a stirring module and a jet flow module are arranged in the shell, the jet flow module is positioned at the bottom of the shell, the stirring module comprises a stirring shaft extending into the shell, and at least one radial flow paddle and at least one axial flow paddle are arranged on the stirring shaft; the method of biological reaction comprises: the reaction product is injected into the shell, the stirring module is started to stir the reaction product, and the gas generated by the reaction enters the jet flow module through the circulating pipeline and is sprayed into the shell through the jet flow module. The invention combines the jet module and the stirring module, so that mass transfer and heat transfer are better realized, reactants are fully mixed and reacted, and gas circulates in the reaction device through the circulating pipeline, so that the production efficiency of the biological reaction device can be effectively improved, and the device has the characteristics of low cost, small occupied area, easiness in maintenance and the like, and can be applied to the fields of food, medicine and the like.

Description

Biological reaction device and biological reaction method

Technical Field

The invention belongs to the technical field of biological reaction, relates to a biological reaction device, and particularly relates to a biological reaction device and a biological reaction method.

Background

Bioreactor technology has shown great economic and social value as an emerging technology, such as anaerobic fermentation for municipal sludge wastewater, beer and various antibiotic production, and the use of fermentation reactors for solid waste treatment. The traditional bioreactor has the problems of low equipment yield, high energy consumption, low reaction rate and the like, and when the stirring is insufficient, the local temperature is extremely high, so that reactants are deteriorated, or air and fermentation liquor cannot be fully mixed, and the supply of dissolved oxygen required by the growth and metabolism of microorganisms cannot be ensured. In addition, the artifical ejection of compact of current fermentation cylinder is wasted time and energy, and reinforced back jar body leakproofness is relatively poor, causes the dust bacterium to get into inside easily, and traditional bioreactor can not satisfy present demand to the material production.

With the continuous increase of the demand of the bioreactor products and the continuous improvement of the biochemical technology, the requirements for the high efficiency and the energy conservation of the bioreactor are higher and higher. However, due to different structures of biomass raw materials, the physical properties, the chemical properties and the fermentation performance of the biomass raw materials have certain differences, the traditional fermentation bioreactor has poor interphase mixing and contact, and the mass transfer capacity of substrates, nutrients and oxygen is low, so that the establishment of a high-efficiency reactor technical platform is the key for solving the problem of high-efficiency utilization of biomass.

CN201459112U discloses a stirring device for fermentation of paenibacillus polymyxa in high viscosity system, which comprises a sealed casing, a stirring shaft disposed in the casing, a turbine oblique blade paddle and one or more airfoil axial flow paddles, wherein the turbine oblique blade paddle and the one or more airfoil axial flow paddles are connected to the stirring shaft, and the stirring shaft has a linkage relationship, wherein the airfoil axial flow paddles are used as upper-layer paddles, and the turbine oblique blade paddle is used as lower-layer paddles. In the stirring device, the axial flow and the radial flow of reactants can be well combined, and the stirring effect is better. But it has a problem that supply of dissolved oxygen required for growth and metabolism of microorganisms cannot be secured.

CN100544808A discloses a method and an apparatus for operating a jet membrane-bioreactor, in which a part of sludge mixed liquid is pressurized by a water pump and then sprayed into the apparatus through a nozzle of a jet device, the formed high-speed water flow sucks ambient air, and a part of air is dissolved into the mixed liquid through mixing and cutting actions to form a gas-liquid jet flow, and the gas-liquid mixed flow drives the sludge mixed liquid in the reactor to form an internal circulation in the reactor, thereby facilitating mass transfer and oxygen transfer, accelerating biochemical reaction, and improving treatment efficiency.

CN101293705A discloses a method for a pulse jet type membrane bioreactor and a device for realizing the method, wherein a jet device is adopted to replace a traditional blower to realize the cross-flow scouring effect on a membrane component, circulating liquid flows through the jet device at a high speed, air enters the jet device in a pulse form through a valve controlled by a PLC (programmable logic controller), and is sprayed at the bottom through a diffusion air distribution device, so that the utilization rate of oxygen is improved, and the sludge degradation effect is improved. However, the generated gas is directly discharged, which affects the environment, and the mixing effect of the reactants is not ideal, and the reaction temperature cannot be controlled.

The existing biological reaction device has the problems of poor mixing effect and insufficient supply of dissolved oxygen, so that how to ensure that the biological reaction device has high-efficiency reaction, and the good mixing effect and the sufficient supply of the dissolved oxygen can be ensured at the same time, thereby becoming the problem which needs to be solved urgently at present.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a biological reaction device and a biological reaction method.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a biological reaction device, which comprises a shell, wherein a stirring module and a jet flow module are arranged in the shell; the stirring module comprises a stirring shaft extending into the shell, and at least one radial flow paddle and at least one axial flow paddle are arranged on the stirring shaft.

The top of the shell is connected with a circulating pipeline, the outlet end of the circulating pipeline is connected with a jet module, and gas in the shell is sprayed into the shell through the circulating pipeline and the jet module.

According to the invention, through arranging the stirring module comprising the radial flow paddle and the axial flow paddle, stirring mass transfer can be carried out efficiently, and the mass transfer rate and the dissolved oxygen rate between reactants are further improved through matching with the jet flow module.

It should be noted that the structure, number and arrangement of the radial paddles and the axial paddles are not specifically required or limited, and those skilled in the art know that the radial paddles and the axial paddles may be reasonably arranged according to the distribution of the reactants in the casing, for example, when the reactants are mainly gathered at the top of the casing, the radial paddles are arranged at the top of the stirring shaft, and the axial paddles are arranged at the bottom.

As a preferred technical solution of the present invention, the radial flow paddle is a parabolic paddle, the parabolic paddle includes a circular disk and at least one parabolic blade disposed along an edge of the circular disk, and a cross section of the parabolic blade is parabolic.

Preferably, the parabolic blades are radially distributed with the stirring shaft as a center.

Preferably, the plane of the opening of the parabolic section of the parabolic blade is perpendicular to the plane of the disc.

The parabolic stirring paddle further strengthens the radial flow of reactants and enhances the mass transfer and heat transfer effects in the reaction process through the parabolic blade section.

Preferably, the axial flow propeller comprises an airfoil type axial flow propeller.

Preferably, the stirring shaft is provided with a parabolic stirring paddle, a first wing type axial flow paddle and a second wing type axial flow paddle from top to bottom in sequence.

The invention has the advantages that the parabolic stirring paddle, the first wing type axial flow paddle and the second wing type axial flow paddle are sequentially arranged from top to bottom, so that the device has good radial gas dispersion capacity and axial mixing capacity in the biological reaction process; if the radial stirring paddles are selected completely, layering can occur in the stirring process, so that the phenomenon of uneven mixing is caused, and the fermentation of microorganisms is influenced.

Preferably, the diameter of the first airfoil type axial flow paddle is larger than the diameter of the second airfoil type axial flow paddle.

According to the invention, the parabolic stirring paddle, the first wing type axial flow paddle and the second wing type axial flow paddle are sequentially arranged from top to bottom, the parabolic stirring paddle is used for enhancing the stirring of reactants, and the first wing type axial flow paddle and the second wing type axial flow paddle with different diameters are used for enhancing the axial flow of the reactants; through the combination of the radial flow paddle and the axial flow paddle, reactants are fully mixed in radial and axial flow directions, the mass transfer effect in the reaction process is further enhanced, and the mass transfer capacity among the reactants, nutrients and dissolved oxygen is better improved. According to the invention, the diameter of the first wing-shaped axial flow paddle is larger than that of the second wing-shaped axial flow paddle, so that the diameter of the stirring paddle is properly reduced, reactants at the bottom can be reacted more fully, and the bottom gas jet efficiency can be improved.

As a preferred technical solution of the present invention, the jet module includes a jet plate and at least one nozzle uniformly disposed on the jet plate.

Preferably, the ejection direction of the nozzle is vertically upward.

According to the invention, through the jet module, the gas is sprayed out from the nozzle in a jet mode, and the generated bubbles can effectively improve the dissolved oxygen in the reactant, so that the biological reaction efficiency is improved, and the biological reaction time is further shortened.

Preferably, a filter screen is arranged above the nozzle in a close fit manner.

The filter screen is arranged above the nozzle in a clinging manner, so that the nozzle is prevented from being blocked, and the abnormal operation of the jet module is avoided.

As a preferable technical scheme of the invention, the circulating pipeline is sequentially provided with a gas purification module and a compressor along the gas flow direction.

According to the invention, the generated gas is purified firstly, and the purified gas enters the jet module after being pressurized by the compressor, so that the generation of bubbles is increased.

It is well known to those skilled in the art that when the reaction performed in the biological reaction apparatus is an aerobic reaction, the air inlet pipe may be connected to the circulation pipe of the present invention to supply oxygen required for the aerobic biological reaction, and those skilled in the art may connect pipes appropriately according to the type of reaction in the biological reaction apparatus.

Preferably, the gas purification module comprises an air filter and an air dryer arranged in sequence along the gas flow direction.

According to the invention, the gas generated by the reaction is sequentially filtered, purified and dried, and the purified gas enters the compressor, so that the normal work of the compressor is ensured.

As a preferable technical scheme of the invention, the radial flow paddle and the axial flow paddle are both detachably connected with the stirring shaft.

Preferably, the radial flow paddle and the axial flow paddle are connected with the stirring shaft through keys.

Preferably, a shaft seal assembly is arranged at the joint of the stirring shaft and the shell.

Preferably, the shaft seal assembly employs a packing-type shaft seal.

According to the invention, the filler type shaft seal is arranged at the joint of the stirring shaft and the shell, so that the sealing performance of the device is enhanced, and the gas leakage generated by reaction is searched, thereby avoiding the pollution to the environment.

As a preferable technical scheme of the invention, the stirring shaft is in transmission connection with a motor.

Preferably, the output shaft of the motor is connected with the stirring shaft through a rigid coupling.

Preferably, a support frame is arranged at the top of the outer wall of the shell and used for fixing the motor and the stirring shaft.

Preferably, a deep groove ball bearing is arranged at the joint of the stirring shaft and the support frame.

In a preferred embodiment of the present invention, the outer wall of the housing is provided with a cooling layer.

The cooling layer is arranged on the outer wall of the shell, so that the reaction temperature in the shell can be controlled, the problem that the reaction rate is influenced by overhigh temperature is avoided, and the problem that the local temperature in the reaction interior is overhigh can be avoided by combining the stirring module and the jet flow module.

It should be noted that the cooling layer may be filled with a cooling medium, the present invention does not specifically require or limit the kind of the cooling medium, and any cooling medium disclosed in the prior art or not disclosed in the new art may be used in the present invention, for example, the cooling layer is filled with cooling water.

Preferably, the top of the shell is provided with a feeding hole, and the bottom of the shell is provided with a discharging hole.

Preferably, the material of the radial flow paddle and the material of the axial flow paddle are 304 stainless steel.

In a second aspect, the present invention also provides a method for performing a biological reaction using the biological reaction apparatus according to the first aspect, the method comprising:

the reaction product is injected into the shell, the stirring module is started to stir the reaction product, and the gas generated by the reaction enters the jet flow module through the circulating pipeline and is sprayed into the shell through the jet flow module.

According to the invention, through the combination of bubbles generated by jet flow and stirring reaction of the stirring module, the reactant and the oxygen are fully contacted and mixed, so that the problems that the oxygen and the reactant cannot be fully mixed and dissolved oxygen required by microorganism growth and metabolism cannot be supplied are solved.

As a preferred embodiment of the present invention, the method of biological reaction specifically comprises:

injecting reactants into the shell from the feed inlet, stirring and reacting under the action of the stirring module, controlling the reaction temperature through the cooling layer, and sequentially feeding gas generated by the reaction into an air dryer and an air filter for purification;

and (II) pressurizing the purified gas by a compressor, spraying the gas into the shell through a nozzle, circulating the gas in the shell through a circulating pipeline, and discharging a product after the reaction is finished.

As a preferred embodiment of the present invention, in the step (I), the reaction temperature is 4 to 150 ℃, for example, 4 ℃, 15 ℃, 30 ℃, 45 ℃, 60 ℃, 75 ℃, 90 ℃, 105 ℃, 120 ℃, 135 ℃ or 150 ℃.

In the present invention, the higher or lower temperature will inhibit the activity of the microorganisms, thereby affecting the efficiency of the entire bioreactor.

Preferably, in step (II), the pressure in the shell is 0-0.4 MPa, for example, 0.05MPa, 0.1MPa, 0.2MPa, 0.3MPa, 0.4 MPa.

The recitation of numerical ranges herein includes not only the above-recited numerical values, but also any numerical values between non-recited numerical ranges, and is not intended to be exhaustive or to limit the invention to the precise numerical values encompassed within the range for brevity and clarity.

Compared with the prior art, the invention has the beneficial effects that:

according to the biological reaction device provided by the invention, the jet flow module is combined with the stirring module, wherein the stirring module adopts two stirring type combinations of the axial flow paddle and the radial flow paddle, so that reactants in the shell are fully mixed and reacted, the dissolved oxygen rate is effectively improved by adopting bubbles generated by the jet flow module, the requirements of uniform oxygen transmission and heat transmission are better met, gas is circulated in the reaction device through the circulating pipeline, the environment pollution caused by the discharge of harmful gas generated by reaction is avoided, and the reaction is more efficient and green. The invention can effectively improve the biological reaction efficiency, can reach more than 82.1 percent, effectively shortens the fermentation time, has the characteristics of low cost, small occupied area, easy maintenance and the like, and can be applied to the fields of food, medicine and the like.

Drawings

FIG. 1 is a schematic view of the internal structure of a bioreactor according to an embodiment of the present invention;

FIG. 2 is a schematic view of the overall structure of a bioreactor according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a parabolic stirring paddle according to an embodiment of the present invention.

Wherein, 1-a stirring shaft; 2-a parabolic stirring paddle; 3-a first airfoil axial flow paddle; 4-a second wing type axial flow paddle; 5-a shell; 6-a fluidic module; 7-an air filter; 8-an air dryer; 9-a circulation pipeline; 10-a compressor; 11-a motor; 12-a rigid coupling; 13-a support frame; 14-deep groove ball bearing; 15-packing type shaft seal; 16-a cooling layer; 17-a discharge hole; 18-a disc; 19-parabolic blade.

Detailed Description

It is to be understood that in the description of the present invention, the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be taken as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

It should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "disposed," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.

In one embodiment, the present invention provides a bioreactor, as shown in fig. 1 and 2, comprising a housing 5, a stirring module disposed in the housing 5, and a fluidic module 6 disposed at the bottom of the housing 5; the stirring module comprises a stirring shaft 1 extending into the shell 5, and at least one radial flow paddle and at least one axial flow paddle are arranged on the stirring shaft 1; the top of the shell 5 is connected with a circulating pipeline 9, the outlet end of the circulating pipeline 9 is connected with a jet module 6, and gas in the shell 5 is sprayed into the shell 5 through the circulating pipeline 9 and the jet module 6.

The radial flow paddle is a parabolic stirring paddle 2, as shown in fig. 3, the parabolic stirring paddle 2 comprises a disk 18 and at least one parabolic blade 19 arranged along the edge of the disk 18, the parabolic blades 19 are radially distributed by taking the stirring shaft 1 as the center, the cross section of the parabolic blade 19 is parabolic, and the plane of the opening of the parabolic cross section of the parabolic blade 19 is perpendicular to the plane of the disk 18.

The jet module 6 comprises a jet plate and at least one nozzle uniformly arranged on the jet plate, wherein the spraying direction of the nozzle is vertical upwards, and furthermore, a filter screen is arranged above and closely attached to the nozzle.

The circulation line 9 is provided with an air filter 7, an air dryer 8 and a compressor 10 in this order along the gas flow direction.

The radial flow paddle and the axial flow paddle are connected with the stirring shaft 1 through keys, and further, a filler type shaft seal 15 is arranged at the joint of the stirring shaft 1 and the shell 5; the stirring shaft 1 is in transmission connection with a motor 11, and further, an output shaft of the motor 11 is connected with the stirring shaft 1 through a rigid coupling 12.

The top of the outer wall of the shell 5 is provided with a support frame 13, the support frame 13 is used for fixing the motor 11 and the stirring shaft 1, and further, a deep groove ball bearing 14 is arranged at the joint of the stirring shaft 1 and the support frame 13.

The outer wall of the shell 5 is provided with a cooling layer 16, the top of the shell 5 is provided with a feeding hole, the bottom of the shell 5 is provided with a discharging hole 17, and further, the material of the radial flow paddle and the material of the axial flow paddle are 304 stainless steel.

In another embodiment, the present invention provides a method for performing a biological reaction using the biological reaction apparatus of one embodiment, the method for performing a biological reaction specifically includes:

injecting a microbial fermentation reactant into the shell 5 from the feed inlet, stirring and reacting under the action of the stirring module, controlling the reaction temperature to be 4-150 ℃ through the cooling layer 16, and sequentially feeding gas generated by the reaction into the air dryer 8 and the air filter 7 for purification;

and (II) pressurizing the purified gas by a compressor 10, spraying the gas into the shell 5 by a nozzle, wherein the pressure in the shell 5 is 0-0.4 Mpa, circularly flowing the gas in the shell 5 through a circulating pipeline 9, and discharging a product from a discharge hole 17 after the reaction is finished.

Example 1

The embodiment provides a biological reaction device, the biological reaction device that provides based on a specific embodiment, wherein, parabolic stirring rake 2, first wing type axial flow rake 3 and second wing type axial flow rake 4 have set gradually from top to bottom on the (mixing) shaft 1, and the diameter of first wing type axial flow rake 3 is greater than the diameter of second wing type axial flow rake 4, evenly is provided with 16 nozzles on the efflux board, all the other structures and a specific embodiment biological reaction device identical.

This example also provides a method for performing a biological reaction using the above biological reaction apparatus, based on a method for performing a biological reaction provided in an embodiment, wherein the reaction temperature is 37 ℃, the pressure in the housing 5 is 0.4MPa, and the remaining operation steps and parameters are the same as those of the method for performing a biological reaction described in an embodiment.

The reaction efficiency data of the bioreactor for performing the biological reaction provided in this example are shown in Table 1.

Example 2

The embodiment provides a biological reaction device, which is based on a biological reaction device provided by a specific embodiment, wherein a first parabolic stirring paddle 2, an airfoil axial flow paddle and a second parabolic stirring paddle 2 are sequentially arranged on a stirring shaft 1 from top to bottom, the diameter of the first parabolic stirring paddle 2 is the same as that of the second parabolic stirring paddle 2, 9 nozzles are uniformly arranged on a jet flow plate, and the rest structures are completely the same as the biological reaction device provided by the specific embodiment.

This example also provides a method for performing a biological reaction using the above biological reaction apparatus, based on a method for performing a biological reaction provided in an embodiment, wherein the reaction temperature is 4 ℃, the pressure in the housing 5 is 0.1MPa, and the remaining operation steps and parameters are the same as those of the method for performing a biological reaction described in an embodiment.

Example 3

The embodiment provides a biological reaction device, the biological reaction device that provides based on a specific embodiment, wherein, first wing type axial flow oar 3, second wing type axial flow oar 4 and parabola type stirring rake 2 have set gradually from top to bottom on the (mixing) shaft 1, and the diameter of first wing type axial flow oar 3 is greater than the diameter of second wing type axial flow oar 4, evenly is provided with 12 nozzles on the efflux board, all the other structures and a specific embodiment biological reaction device identical.

This example also provides a method for performing a biological reaction using the above biological reaction apparatus, based on a method for performing a biological reaction provided in an embodiment, wherein the reaction temperature is 60 ℃, the pressure in the housing 5 is 0.05MPa, and the remaining operation steps and parameters are the same as those of the method for performing a biological reaction described in an embodiment.

Example 4

The embodiment provides a biological reaction device, and is based on a biological reaction device provided by a specific embodiment, wherein an airfoil axial flow paddle and a parabolic stirring paddle 2 are sequentially arranged on a stirring shaft 1 from top to bottom, the diameter of the airfoil axial flow paddle is equal to that of the parabolic stirring paddle 2, 25 nozzles are uniformly arranged on a jet flow plate, and the rest structures are completely the same as the biological reaction device provided by a specific embodiment.

This example also provides a method for performing a biological reaction using the above biological reaction apparatus, based on a method for performing a biological reaction provided in an embodiment, wherein the reaction temperature is 90 ℃, the pressure in the housing 5 is 0.3MPa, and the remaining operation steps and parameters are the same as those of the method for performing a biological reaction described in an embodiment.

Example 5

The embodiment provides a biological reaction device, the biological reaction device that provides based on a specific embodiment, wherein, from top to bottom set gradually first wing type axial flow oar 3, first parabola type stirring rake 2, second wing type axial flow oar 4 and second parabola type stirring rake 2 on the (mixing) shaft 1, the diameter of first wing type axial flow oar 3 is greater than the diameter of second wing type axial flow oar 4, the diameter of first parabola type stirring rake 2 is greater than the diameter of second parabola type stirring rake 2, evenly is provided with 24 nozzles on the efflux board, all the other structures and a specific embodiment biological reaction device identical.

This example also provides a method for performing a biological reaction using the above biological reaction apparatus, based on a method for performing a biological reaction according to an embodiment, wherein the reaction temperature is 150 ℃, the pressure in the housing 5 is 0.15MPa, and the remaining operation steps and parameters are the same as those of the method for performing a biological reaction according to an embodiment.

Comparative example 1

This comparative example provides a biological reaction device, and the difference with embodiment 1 lies in, first wing type axial-flow oar 3, second wing type axial-flow oar 4 and third wing type axial-flow oar have set gradually from top to bottom on the (mixing) shaft 1, and the diameter of first wing type axial-flow oar 3, second wing type axial-flow oar 4 and third wing type axial-flow oar reduces in proper order, and all the other structures, reaction steps and parameter all are the same with embodiment 1.

The reaction efficiency data of the biological reaction performed by the biological reaction apparatus provided in this comparative example are shown in Table 1.

Comparative example 2

This comparative example provides a biological reaction apparatus, and the difference with embodiment 1 lies in, first parabola type stirring rake 2, second parabola type stirring rake 2 and third parabola type stirring rake 2 have been set gradually from top to bottom on the (mixing) shaft 1, and the diameter of first parabola type stirring rake 2, second parabola type stirring rake 2 and third parabola type stirring rake 2 reduces in proper order, and all the other structures, reaction step and parameter are the same with embodiment 1.

Comparative example 3

This comparative example provides a bioreactor, which is different from example 1 in that it does not have the fluidic module 6, and the rest of the structure, reaction steps and parameters are the same as those of example 1.

TABLE 1

	Efficiency of reaction
		Example 1	88.6％
Example 2	82.1％
		Example 3	85.3％
Example 4	84.8％
		Example 5	82.5％
Comparative example 1	72.7％
		Comparative example 2	75.6％
Comparative example 3	70.5％

As can be seen from the data in table 1:

(1) in example 1, compared with examples 2, 3, 4 and 5, the microorganism fermentation is mainly concentrated on the upper part in the shell 5, so the radial flow paddle is arranged on the upper part of the stirring shaft 1, which is beneficial to fully mixing reactants, thereby having higher reaction efficiency.

(2) Compared with comparative examples 1 and 2, the reaction efficiency of example 1 is obviously higher than that of comparative examples 1 and 2, and the axial flow paddle and the radial flow paddle are combined to simultaneously have good radial gas dispersing capacity and axial mixing capacity in the biological reaction process; if the radial stirring paddles are selected completely, layering can occur in the stirring process, so that the phenomenon of uneven mixing is caused, and the fermentation of microorganisms is influenced.

(3) Compared with the comparative example 3, the reaction efficiency of the example 1 is obviously higher than that of the comparative example 3, the effect of generating bubbles is enhanced by the aid of the jet module 6 through combined action of the jet module 6 and the stirring module, and mass transfer and heat transfer effects of reactants are further enhanced by combination of generated jet flow turbulence and stirring, so that the reaction efficiency is effectively improved.

According to the biological reaction device provided by the invention, the jet flow module 6 is combined with the stirring module, wherein the stirring module adopts two stirring type combinations of the axial flow paddle and the radial flow paddle, so that reactants in the shell 5 are fully mixed and reacted, the dissolved oxygen rate is effectively improved by adopting bubbles generated by the jet flow module 6, the requirements of uniform oxygen transfer and heat transfer are better met, the gas is circulated in the reaction device through the circulating pipeline 9, the environment pollution caused by the discharge of harmful gas generated by reaction is avoided, and the reaction is more efficient and green. The invention can effectively improve the biological reaction efficiency and shorten the fermentation time, has the characteristics of low cost, small occupied area, easy maintenance and the like, and can be applied to the fields of food, medicine and the like.

The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.

Claims

1. The biological reaction device is characterized by comprising a shell, wherein a stirring module and a jet flow module are arranged in the shell; the stirring module comprises a stirring shaft extending into the shell, and at least one radial flow paddle and at least one axial flow paddle are arranged on the stirring shaft;

2. The bioreactor apparatus according to claim 1, wherein the radial paddles are parabolic paddles comprising a circular disk and at least one parabolic blade disposed along the edge of the circular disk, the parabolic blade having a parabolic cross-section;

preferably, the parabolic blades are radially distributed by taking the stirring shaft as a center;

preferably, the plane of the opening of the parabolic section of the parabolic blade is perpendicular to the plane of the disc;

preferably, the axial flow propeller comprises an airfoil-shaped axial flow propeller;

preferably, the stirring shaft is provided with a parabolic stirring paddle, a first wing type axial flow paddle and a second wing type axial flow paddle from top to bottom in sequence;

3. The bioreactor of claim 1 or 2, wherein the fluidic module comprises a fluidic plate and at least one nozzle uniformly disposed on the fluidic plate;

preferably, the spraying direction of the nozzle is vertically upward;

preferably, a filter screen is arranged above the nozzle in a close fit manner.

4. The bioreactor according to any one of claims 1 to 3, wherein a gas purification module and a compressor are provided in this order along the gas flow direction on the circulation line;

5. The bioreactor according to any one of claims 1 to 4, wherein the radial paddles and the axial paddles are detachably connected to the stirring shaft;

preferably, the radial flow paddle and the axial flow paddle are connected with the stirring shaft through keys;

preferably, a shaft seal assembly is arranged at the joint of the stirring shaft and the shell;

preferably, the shaft seal assembly employs a packing-type shaft seal.

6. The bioreactor apparatus according to any one of claims 1 to 5, wherein the stirring shaft is in driving connection with a motor;

preferably, the output shaft of the motor is connected with the stirring shaft through a rigid coupling;

preferably, a support frame is arranged at the top of the outer wall of the shell and used for fixing the motor and the stirring shaft;

7. The bioreactor apparatus according to any one of claims 1 to 6, wherein the outer wall of the housing is provided with a cooling layer;

preferably, the top of the shell is provided with a feed inlet, and the bottom of the shell is provided with a discharge outlet;

8. A method for performing a biological reaction using the biological reaction apparatus of any one of claims 1 to 7, wherein the biological reaction method comprises:

9. The method of bioreaction according to claim 8, wherein the method of bioreaction comprises in particular:

10. The method of claim 9, wherein in step (I), the reaction temperature is 4 to 150 ℃;

preferably, in the step (II), the pressure in the shell is 0-0.4 MPa.