CN206127324U - Industrialization immobilized enzyme response device - Google Patents
Industrialization immobilized enzyme response device Download PDFInfo
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- CN206127324U CN206127324U CN201621071509.6U CN201621071509U CN206127324U CN 206127324 U CN206127324 U CN 206127324U CN 201621071509 U CN201621071509 U CN 201621071509U CN 206127324 U CN206127324 U CN 206127324U
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- coil pipe
- filter
- industrialization
- immobilized enzyme
- temperature control
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Abstract
The utility model discloses an industrialization immobilized enzyme response device, which comprises a tank body, the jar body still includes the agitator including manhole cover, barrel and bottom, the agitator includes (mixing) shaft and stirring leaf, and the (mixing) shaft is located a jar internal axle center position, the fixed stirring leaf of (mixing) shaft lower extreme, the utility model aims at providing an industrialization immobilized enzyme response device can guarantee immobilized enzyme and feed liquid intensive mixing, makes the enzyme live and exerts the optimum, and it is damaged minimum to enzyme carrier to guarantee to stir mixing equipment again, can shift the feed liquid come out thoroughly fast after the reaction, and this response device convenience was washd and was disappeared the while, did not have the dead angle.
Description
Technical field
This utility model is related to bioreactor, more particularly to a kind of industrialization immobilized enzyme consersion unit.
Background technology
Immobilized enzyme catalysis react in industrialized production that extensively, its advantage is fairly obvious using increasingly, and it can be
Under relatively mild reaction condition, a step completes the conversion that can just complete by multi-step chemical reaction, and not only reaction efficiency is high, specificity
By force, and reaction typically complete in water phase, do not use organic solvent, it is very environmentally friendly.But immobilized enzyme price general charged compares
Costliness, use cost are higher, therefore, it is highly desirable to develop a kind of suitable industrialization immobilized enzyme consersion unit, can meet
Catalytic reaction condition needs, and can guarantee that the destruction to immobilized enzyme is minimum again, and service life is most long, to reduce use cost.
Utility model content
This utility model aims to provide a kind of industrialization immobilized enzyme consersion unit, both can guarantee that immobilized enzyme and feed liquid were filled
Dividing mixing, playing enzyme activity optimum, can guarantee that again and mixing apparatus are stirred to the damaged minimum of zymophore, reaction can be by feed liquid after terminating
Quickly thoroughly it is transferred out, while the consersion unit facilitates cleaning to disappear, no dead angle.
To reach above-mentioned purpose, the technical solution adopted in the utility model is as follows：
A kind of industrialization immobilized enzyme consersion unit, including tank body, the tank body include manhole cover, cylinder and bottom, also
Including agitator, the agitator includes shaft and paddle, and shaft is located at the shaft core position in tank body, shaft lower end
Fixed paddle；
The cylinder includes successively the axially upper cover of connection, straight barrel and low head, is provided with feed supplement on the upper cover
Hole and manhole, the manhole cover are located at manhole outside opening and are connected with cylinder, are fixed with ring flange on the outside opening of feed supplement hole,
Feed supplement hole inside opening is connected with feed supplement coil pipe, and the cleaning coil pipe with rotating wash bowl, the feed supplement are provided with the downside of the feed supplement coil pipe
Coil pipe and cleaning coil pipe are each attached on the inwall of upper cover；
Baffle plate is fixed with the straight barrel inwall, side discharging opening is provided with straight barrel lower sides, inside the discharging opening of side
Opening is connected with upper level filter element kit, and side discharging opening is located at below baffle plate；Waste discharge mouth is provided with the wall of the low head side, under
End socket bottom is provided with the through hole being adapted to bottom；Bottom discharge mouth, bottom discharge mouth inside opening and bottom are provided with the bottom
Filter the connection of filter disc component in portion；The bottom is fixed on cylinder bottom.
Also include power set, the power set include reductor, frequency conversion motor, shaft coupling and support, the frequency conversion
Motor and speed reducer is fixed on cylinder by support；Shaft upper end is exported with reductor by shaft coupling at the top of cylinder
Axle connects.
Preferably, the upper level filter element kit includes discharge nozzle, filter element, filter element top fixing bolt and filter element front end
Fixed support, the filter element include filter element interface, filtering surface and filter element top fixing nut；Filter element one end is fixed on filter element front end
On fixed support, the filter element other end is connected with discharge nozzle.
Preferably, filter disc component is filtered in the bottom includes that filtering table and filter disc support spoke, the filter disc to support spoke
It is arranged on bottom inwall, filtering table is bolted on bottom.
Preferably, also including automatic temperature control system, the automatic temperature control system includes straight barrel temperature control outer coil pipe, low head
Temperature control outer coil pipe, controller, temperature sensor and two regulating valves, the temperature sensor is installed in apart from cylinder body bottom 10-
30cm positions, two regulating valves are located on the outer coil pipe of straight barrel temperature control, the outer coil pipe of low head temperature control respectively, temperature sensor, tune
Section valve is electrically connected with the controller.
Preferably, the outer coil pipe of the straight barrel temperature control is arranged on straight barrel outer wall, the outer coil inlets of straight barrel temperature control
Below the outer coil pipe outlet of straight barrel temperature control；The outer coil pipe of the low head temperature control is arranged on low head outer wall, low head
The outer coil inlets of temperature control are located at below the outer coil pipe outlet of low head temperature control.
Preferably, automatic control feeding-system includes controller, electromagnetic valve and PH sensors, and the PH sensors are arranged on distance
Cylinder body bottom 10-30cm positions, electromagnetic valve are arranged on feed supplement coil pipe, and PH sensors, electromagnetic valve are electrically connected with the controller.
Preferably, the agitator is four leaf propeller mixer of Flos Mume leaf variable cross-section.
Preferably, the upper level filter element kit includes at least two groups filter elements.
Preferably, the filtering table is flat filter disc.
Agitator selects four leaf propeller mixer of Flos Mume leaf variable cross-section, with very big DIFFUSION IN TURBULENCE ability and relatively low
Shearing force；Enzyme can be made to suspend and be homogeneously mixed in solution, give full play to the effect of enzyme, improve the respond of enzyme.
Adjust agitator speed to control mixing effect and shearing force using converter, it is ensured that the activity of enzyme, enzyme can be made
Respond perform to maximum.
Discharge filtering device was both can ensure that by the way of upper level filter element kit and bottom filter disc component combine
Quickly discharging, can ensure that discharging is thorough again；If need to be passed through gas (such as air, oxygen etc.), filter element when reacting simultaneously
Gas distributor is also acted as with filter disc.
Upper level filter element kit adopts one group or multigroup filter core, it is ensured that have enough filter areas, to ensure
The rate of filtration.Filtered using flat filter disc consersion unit bottom, it is ensured that will be the discharge of completely reacted feed liquid clean.
There is the cleaning coil pipe with rotating wash bowl at the top of consersion unit, under the drive of cleanout fluid fluid, rotating wash bowl can enter
360 ° of rotations of row, it is ensured that consersion unit inner wall washing is clean, without dead angle, rotating wash bowl number generally 2～4.
Feed supplement coil pipe is provided with the top of consersion unit, uniform discharge opening on coil pipe needs the thing added when making enzymic catalytic reaction
Material can relatively evenly fall reactant liquor surface.
Direct tube section, low head and its internals inside consersion unit, including stirring, baffle plate, element kit support etc.,
It is processed by shot blasting, without sharp square edge, the shearing that equipment is caused to immobilized enzyme when reducing stirring reaction to greatest extent
Destruction.
Consersion unit using when generally be equipped with automatic control feeding-system, for example, by the PH robot control system(RCS)s for configuring, constantly mend
Enter diluted acid or diluted alkaline so that the pH value of reaction system keeps stable.
Consersion unit using when generally be equipped with automatic temperature control system, by the flow for controlling outer coil pipe inner cold water or hot water
To keep the temperature stabilization of reaction system.
In addition, being provided with charging aperture, water inlet (cleaning use) on equipment upper cover as needed, feed supplement import, evacuation port, regarding
The function mouth of pipe such as mirror.
Filter element material can for rustless steel sintered meshwork, titanium rod filter element and other can reuse filter medium.
Compared with prior art, the beneficial effects of the utility model are：Agitator revolves oar from four leaf of Flos Mume leaf variable cross-section
Formula agitator, while adjusting agitator speed using converter, both can guarantee that good solid-liquid mixed effect, had made immobilized enzyme
It is uniformly suspended within reaction solution, gives full play to the effect of enzyme, can guarantee that very little is sheared again, improves the service life of enzyme.
Reaction terminates quickly thoroughly to be transferred out feed liquid.
Description of the drawings
Fig. 1 is this utility model structural representation
Fig. 2 is upper level filter element kit distribution schematic diagram
Fig. 3 is upper level filter element kit top view
Fig. 4 is upper level filter filter element schematic diagram,
Fig. 5 is lower floor's filter assemblies distribution schematic diagram
Fig. 6 is bottom floor map
In figure：1- frequency conversion motors, 2- reductors, 3- shaft couplings, 4- supports, 5- manholes, 6- feed supplement coil pipes, 7- cleaning disks
Pipe, 8- shafts, 9- paddles, 10- sides discharging opening, 11- straight barrels temperature control outer coil inlets, 12- low head temperature control outer disks
Outside the outer coil inlets of pipe outlet, 13- low heads temperature control, 14- through holes, 15- waste discharge mouths, 16- baffle plates, 17- straight barrel temperature controls
Coil pipe outlet, 18- discharge nozzles, 19- filter elements, 20- filter elements top fixing bolt, 21- filter elements front end fixed support, 22- filter elements
Interface, 23- filtering surfaces, 24- filter elements top fixing nut, 25- filtering tables, 26- bottoms, 27- bottom discharge mouths, 28- manhole covers,
29- filter discs support spoke, 30- cylinders, 31- upper covers, 32- low heads, 33- straight barrels, 34- feed supplements hole, 35- straight barrel temperature controls
The outer coil pipe of outer coil pipe, 36- low heads temperature control.
Specific embodiment
In order that the purpose of this utility model, technical scheme and advantage become more apparent, below in conjunction with accompanying drawing, to this reality
It is further elaborated with new.
As shown in figures 1 to 6, a kind of industrialization immobilized enzyme consersion unit, including tank body, the tank body include manhole cover 28,
Cylinder 30 and bottom 26, also including agitator, the agitator includes shaft 8 and paddle 9, and shaft 8 is located in tank body
Shaft core position, 8 lower end of shaft fix paddle 9；
The cylinder 30 includes successively the axially upper cover 31 of connection, straight barrel 33 and low head 32, the upper cover 31
On be provided with feed supplement hole 34 and manhole 5, the manhole cover 28 is located at 5 outside opening of manhole and is connected with cylinder 30, outside feed supplement hole 34
Ring flange is fixed with portion's opening, and 34 inside opening of feed supplement hole is connected with feed supplement coil pipe 6, band is provided with the downside of the feed supplement coil pipe 6
The cleaning coil pipe 7 of rotating wash bowl, the feed supplement coil pipe 6 and cleaning coil pipe 7 are each attached on the inwall of upper cover 31；
Baffle plate 16 is fixed with 33 inwall of the straight barrel, on 33 lower sides of straight barrel, side discharging opening 10 is provided with, side goes out
10 inside opening of material mouth is connected with upper level filter element kit, and side discharging opening 10 is located at below baffle plate 16；32 side of the low head
Waste discharge mouth 15 is provided with wall, 32 bottom of low head is provided with the through hole 14 being adapted to bottom 26；Bottom is provided with the bottom 26 to go out
Material mouth 27,27 inside opening of bottom discharge mouth filter filter disc component with bottom and are connected；The bottom 26 is fixed on 30 bottom of cylinder.
Preferably, also including power set, the power set include reductor 2, frequency conversion motor 1, shaft coupling 3 and support
4, the frequency conversion motor 1 and reductor 2 are fixed on cylinder 30 by support 4；8 upper end of shaft passes through at the top of cylinder 30
Shaft coupling 3 is connected with 2 output shaft of reductor.
Preferably, the upper level filter element kit includes discharge nozzle 18, filter element 19, filter element top fixing bolt 20 and filter
Core front end fixed support 21, the filter element 19 include filter element interface 22, filtering surface 23 and filter element top fixing nut 24；Filter element 19
One end is fixed on filter element front end fixed support 21, and 19 other end of filter element is connected with discharge nozzle 18.
Preferably, filter disc component is filtered in the bottom includes that filtering table 25 and filter disc support spoke 29, the filter disc to support
Spoke 29 is arranged on 26 inwall of bottom, and filtering table 25 is bolted on bottom 26.
Preferably, also including automatic temperature control system, the automatic temperature control system includes straight barrel temperature control outer coil pipe 35, lower envelope
Head temperature control outer coil pipe 36, controller, temperature sensor and two regulating valves, the temperature sensor is installed in apart from 30 bottom of cylinder
Portion 10-30cm positions, two regulating valves are located on the outer coil pipe 35 of straight barrel temperature control, the outer coil pipe 36 of low head temperature control respectively, temperature
Sensor, regulating valve are electrically connected with the controller.
Preferably, the outer coil pipe 35 of the straight barrel temperature control is arranged on 33 outer wall of straight barrel, and the outer coil pipe of straight barrel temperature control enters
The mouth of a river 11 is located at outside straight barrel temperature control below coil pipe outlet 17；The outer coil pipe 36 of the low head temperature control is arranged on outside low head 32
On wall, the outer coil inlets 12 of low head temperature control are located at outside low head temperature control below coil pipe outlet 13.
Preferably, also including automatic control feeding-system, automatic control feeding-system includes controller, electromagnetic valve and PH sensors, institute
PH sensors are stated installed in apart from cylinder body bottom 10-30cm positions, electromagnetic valve is arranged on feed supplement coil pipe 6, PH sensors, electromagnetism
Valve is electrically connected with the controller.The automatic control feeding-system can constantly fill into diluted acid or diluted alkaline by feed supplement coil pipe 6 and make reaction
The pH value of system keeps stable.
Preferably, the agitator is four leaf propeller mixer of Flos Mume leaf variable cross-section.
Preferably, the upper level filter element kit includes at least two groups filter elements 19.
Preferably, the filtering table 25 is flat filter disc.
Agitator selects four leaf propeller mixer of Flos Mume leaf variable cross-section, with very big DIFFUSION IN TURBULENCE ability and relatively low
Shearing force；Enzyme can be made to suspend and be homogeneously mixed in solution, give full play to the effect of enzyme, improve the respond of enzyme.
Adjust agitator speed to control mixing effect and shearing force using converter, it is ensured that the activity of enzyme, enzyme can be made
Respond perform to maximum.
Discharge filtering device was both can ensure that by the way of upper level filter element kit and bottom filter disc component combine
Quickly discharging, can ensure that discharging is thorough again；If need to be passed through gas (such as air, oxygen etc.), filter element when reacting simultaneously
Gas distributor is also acted as with filter disc.
Upper level filter element kit adopts one group or multigroup filter core, it is ensured that have enough filter areas, to ensure
The rate of filtration.Filtered using flat filter disc consersion unit bottom, it is ensured that will be the discharge of completely reacted feed liquid clean.
There is the cleaning coil pipe with rotating wash bowl at the top of consersion unit, under the drive of cleanout fluid fluid, rotating wash bowl can enter
360 ° of rotations of row, it is ensured that consersion unit inner wall washing is clean, without dead angle, rotating wash bowl number generally 2～4.
Feed supplement coil pipe is provided with the top of consersion unit, uniform discharge opening on coil pipe needs the thing added when making enzymic catalytic reaction
Material can relatively evenly fall reactant liquor surface.
Direct tube section, low head and its internals (including stirring, baffle plate, element kit support etc.) inside consersion unit
It is processed by shot blasting, without sharp square edge, the shearing that equipment is caused to immobilized enzyme when reducing stirring reaction to greatest extent
Destruction.
Consersion unit using when generally be equipped with automatic control feeding-system, for example, by the PH robot control system(RCS)s for configuring, constantly mend
Enter diluted acid or diluted alkaline so that the pH value of reaction system keeps stable.
Consersion unit using when generally be equipped with automatic temperature control system, by the flow for controlling outer coil pipe inner cold water or hot water
To keep the temperature stabilization of reaction system.
In addition, being provided with charging aperture, water inlet (cleaning use) on equipment upper cover as needed, feed supplement import, evacuation port, regarding
The function mouth of pipe such as mirror.
Filter element material can for rustless steel sintered meshwork, titanium rod filter element and other can reuse filter medium.
Equipment method of work：
Step 1：This equipment is first cleaned up with diluted acid, diluted alkaline and purified water using front, is then gone out with steam again
Bacterium is lowered the temperature standby after processing.
Step 2：The immobilized enzyme of quantity is needed according to technological requirement from manhole input, then with purified water or other bufferings
Liquid is standby after immobilized enzyme is cleaned up.
Step 3：Substrate solution after preparation is proceeded in consersion unit, stirring is opened, and is set appropriate mixing speed,
Opening temperature robot control system(RCS), unlatching automatic feeding system starts feed supplement carries out catalytic reaction.
Step 4：At the end of process control needs, catalytic reaction, automatic feeding and automatic temperature control system are closed, stopped
Completely reacted feed liquid is transferred out by stirring by pressurization or dehvery pump, and when turning material starting, upper and lower filter assemblies are made simultaneously
With being discharged, when upper strata element kit spills, its corresponding discharging opening valve is closed successively, until finally by tank bottoms mistake
Feed liquid is thoroughly gone out totally by filter component.
Step 5：Repeat step 3 and the operation required by step 4, reach the target of continuous industrial production.
If running into short time production interval, after can cleaning up to immobilized enzyme in the equipment, fixation is made
Change the bottom that enzyme concentrates on equipment, cryopreservation is carried out by low head temperature control coil pipe.
When the activity of immobilized enzyme is less than use requirement, you can stop using, by the waste discharge mouth of adjacent bottom filter disc
Useless enzyme is released.
As the rate of filtration of the increase using batch, filter element and filter disc can be reduced, on-line cleaning can be carried out to them,
Them can be taken apart if necessary carries out strengthening cleaning to recover permeability.
Certainly, this utility model can also have other various embodiments, spiritual and its essence without departing substantially from this utility model
In the case of, those of ordinary skill in the art can make various corresponding changes and deformation, but these phases according to this utility model
The change and deformation answered should all belong to the scope of the claims appended by this utility model.
Claims (10)
1. a kind of industrialization immobilized enzyme consersion unit, including tank body, it is characterised in that：The tank body includes manhole cover, cylinder
And bottom, also including agitator, the agitator includes shaft and paddle, and shaft is located at the shaft core position in tank body,
Paddle is fixed in shaft lower end；
The cylinder includes successively the axially upper cover of connection, straight barrel and low head, be provided with the upper cover feed supplement hole and
Manhole, the manhole cover are located at manhole outside opening and are connected with cylinder, are fixed with ring flange, feed supplement on the outside opening of feed supplement hole
Hole inside opening is connected with feed supplement coil pipe, and the cleaning coil pipe with rotating wash bowl, the feed supplement coil pipe are provided with the downside of the feed supplement coil pipe
It is each attached on the inwall of upper cover with cleaning coil pipe；
Baffle plate is fixed with the straight barrel inwall, side discharging opening, side discharging opening inside opening on straight barrel lower sides, is provided with
It is connected with upper level filter element kit, side discharging opening is located at below baffle plate；Waste discharge mouth, low head are provided with the wall of the low head side
Bottom is provided with the through hole being adapted to bottom；Bottom discharge mouth, bottom discharge mouth inside opening and bottom mistake are provided with the bottom
The component connection of filter filter disc；The bottom is fixed on cylinder bottom.
2. a kind of industrialization immobilized enzyme consersion unit according to claim 1, it is characterised in that：Also fill including power
Put, the power set include that reductor, frequency conversion motor, shaft coupling and support, the frequency conversion motor and reductor pass through support
It is fixed on cylinder；The shaft upper end is connected with speed reducer output shaft by shaft coupling at the top of cylinder.
3. a kind of industrialization immobilized enzyme consersion unit according to claim 1, it is characterised in that：The upper level filter filter
Core assembly includes discharge nozzle, filter element, filter element top fixing bolt and filter element front end fixed support, and the filter element includes that filter element connects
Mouth, filtering surface and filter element top fixing nut；Filter element one end is fixed on the fixed support of filter element front end, the filter element other end with discharging
Pipe is connected.
4. a kind of industrialization immobilized enzyme consersion unit according to claim 1, it is characterised in that：Filter filter in the bottom
Disk component includes filtering table and filter disc supports spoke, the filter disc to support spoke to be arranged on bottom inwall, and filtering table passes through spiral shell
Bolt is fixed on bottom.
5. a kind of industrialization immobilized enzyme consersion unit according to claim 1, it is characterised in that：Also include temp autocontrolled
System, the automatic temperature control system include the outer coil pipe of straight barrel temperature control, low head temperature control outer coil pipe, controller, temperature sensor
With two regulating valves, installed in apart from cylinder body bottom 10-30cm positions, two regulating valves are located at directly respectively for the temperature sensor
On the outer coil pipe of cylinder temperature control, the outer coil pipe of low head temperature control, temperature sensor, regulating valve are electrically connected with the controller.
6. a kind of industrialization immobilized enzyme consersion unit according to claim 5, it is characterised in that：The straight barrel temperature control
Outer coil pipe is arranged on straight barrel outer wall, and the outer coil inlets of straight barrel temperature control are located under the outer coil pipe outlet of straight barrel temperature control
Side；The outer coil pipe of the low head temperature control is arranged on low head outer wall, and the outer coil inlets of low head temperature control are located at low head control
Below the outer coil pipe outlet of temperature.
7. a kind of industrialization immobilized enzyme consersion unit according to claim 5, it is characterised in that：Also include automatic control feed supplement
System, automatic control feeding-system include controller, electromagnetic valve and PH sensors, and the PH sensors are installed in apart from cylinder body bottom
10-30cm positions, electromagnetic valve are arranged on feed supplement coil pipe, and PH sensors, electromagnetic valve are electrically connected with the controller.
8. a kind of industrialization immobilized enzyme consersion unit according to claim 1, it is characterised in that：The agitator is prunus mume (sieb.) sieb.et zucc.
Four leaf propeller mixer of floral leaf variable cross-section.
9. a kind of industrialization immobilized enzyme consersion unit according to claim 3, it is characterised in that：The upper level filter filter
Core assembly includes at least two groups filter elements.
10. a kind of industrialization immobilized enzyme consersion unit according to claim 4, it is characterised in that：The filtering table is
Flat filter disc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621071509.6U CN206127324U (en) | 2016-09-22 | 2016-09-22 | Industrialization immobilized enzyme response device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621071509.6U CN206127324U (en) | 2016-09-22 | 2016-09-22 | Industrialization immobilized enzyme response device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206127324U true CN206127324U (en) | 2017-04-26 |
Family
ID=58569524
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621071509.6U Active CN206127324U (en) | 2016-09-22 | 2016-09-22 | Industrialization immobilized enzyme response device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206127324U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122445A (en) * | 2021-05-06 | 2021-07-16 | 南京普瑞特生物科技有限公司 | Equipment for preparing optically pure 1- (1-naphthyl) ethylamine by splitting with immobilized enzyme method and use method |
| CN115300980A (en) * | 2022-08-19 | 2022-11-08 | 嵊州市浙江工业大学创新研究院 | Two unification stirred tanks of rotary filter formula |
-
2016
- 2016-09-22 CN CN201621071509.6U patent/CN206127324U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122445A (en) * | 2021-05-06 | 2021-07-16 | 南京普瑞特生物科技有限公司 | Equipment for preparing optically pure 1- (1-naphthyl) ethylamine by splitting with immobilized enzyme method and use method |
| CN115300980A (en) * | 2022-08-19 | 2022-11-08 | 嵊州市浙江工业大学创新研究院 | Two unification stirred tanks of rotary filter formula |
| CN115300980B (en) * | 2022-08-19 | 2024-04-02 | 嵊州市浙江工业大学创新研究院 | Rotary filtering type two-in-one stirring kettle |
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