CN112535645A

CN112535645A - Application of natamycin in preparing shampoo and hair care product for removing dandruff and relieving itching

Info

Publication number: CN112535645A
Application number: CN202011399083.8A
Authority: CN
Inventors: 张涛; 曾莉萍; 杨菁; 雷聪
Original assignee: Chengdu Medical College
Current assignee: Chengdu Medical College
Priority date: 2020-12-01
Filing date: 2020-12-01
Publication date: 2021-03-23

Abstract

The invention belongs to the technical field of daily chemical products, and particularly relates to an application of natamycin in preparing a shampoo and hair care product for removing dandruff and relieving itching. Aiming at the problems of scalp irritation, insecurity and the like of the existing anti-dandruff and itching-relieving medicine, the invention provides the application of natamycin in preparing anti-dandruff and itching-relieving hair washing and protecting products. And, according to the use, the present invention provides a new product for removing dandruff and relieving itching. The invention provides a new choice for the development of dandruff removing hair washing and protecting products, can achieve the effects of removing dandruff and relieving itching under the condition of reducing scalp irritation, and has obvious practical value.

Description

Application of natamycin in preparing shampoo and hair care product for removing dandruff and relieving itching

Technical Field

The invention belongs to the technical field of daily chemical products, and particularly relates to an application of natamycin in preparing a shampoo and hair care product for removing dandruff and relieving itching.

Background

Dandruff is the most common disease of scalp, is called as pityriasis capitis medically, and is clinically represented by excessive fine, grey, dry or slightly greasy pityrospermy-like scraps on the scalp or hair, which can be accompanied by pruritus and has high morbidity of people. Dandruff is a product of metabolism of epidermal cells of the human head. Dandruff is produced by the keratinization of the epidermal cells of the head, and these dead and exfoliated epidermal cells are dandruff. According to the data investigated by the scalp health research center of the Chinese health education association, the scalp of more than 60% of the adults in our country have different health problems, wherein dandruff is one of the main problems.

Abnormal keratinization of epidermal cells, hyperseborrhea caused by endocrine action, and abnormal proliferation of microorganisms in the scalp, which are the root causes of dandruff, are the three factors of dandruff production. Bacterial and fungal infections such as pityriasis, propionibacteria, staphylococci, epidermophyton and candida have been considered as one of the major factors in dandruff formation, particularly in connection with a fungus called malassezia furfur. With the increasingly clarified mechanism of malassezia fungus dandruff, modern anti-dandruff shampoos take various antifungal drugs as the main effective components in the formula of the anti-dandruff shampoo, and the main anti-dandruff additives in the market at present are: salicylic acid, pyridone, ethanolamine, hydroxypyrazole, Zinc Pyrithione (ZPT), climbazole, ketoconazole, triclosan, etc., the most commonly used of which is ZPT, although it is effective in inhibiting the production of dandruff, has limited use due to its stimulating effect on the scalp or hair follicles. Therefore, the development of a novel, safe and nontoxic anti-dandruff drug has become a technical problem in the field at present.

Natamycin, also known as natamycin, is a polyene antibiotic, is a strong antifungal drug and can effectively inhibit the growth of fungi such as yeast and the like. Because of the characteristics of low dosage, high efficiency, wide pH range, strong specificity, high safety and the like, the preparation method is widely applied to the production and the preservation of food such as dairy products, meat and the like. Existing reports show that: the natamycin can be used for treating fungal infection of vagina and lung, is very effective to children skin mucosa infection caused by fungi, and has good treatment effect on fungal keratitis and cuticle inflammation. At present, no report on the effect of natamycin on malassezia is found.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the existing medicine for removing dandruff and relieving itching has the problems of stimulating the scalp or hair follicles and having certain toxic effect.

The technical scheme for solving the technical problems comprises the following steps: provides the application of natamycin in preparing a shampoo and hair care product for removing dandruff and relieving itching.

Wherein, in the application, the hair washing and caring product comprises at least one of shampoo, hair conditioner, hair rinse and hair caring essential oil.

Wherein, in the application, the dosage of the natamycin is not less than 16 mg/mL.

Wherein, in the application, the natamycin can play a role in removing dandruff and relieving itching by inhibiting malassezia furfur.

Furthermore, the invention also provides application of natamycin in inhibiting malassezia furfur.

The invention also provides a hair washing and caring product, which comprises natamycin.

Further, the dosage of the natamycin is more than or equal to 16 mg/mL.

Wherein the hair washing and caring product comprises at least one of shampoo, hair conditioner, hair rinse and hair caring essential oil.

The invention has the beneficial effects that:

the invention discovers that the natamycin has specific inhibition effect on malassezia furfur and human scalp bacteria microorganisms through an in-vitro drug sensitive experiment, thereby providing a new application of the natamycin in preparing a shampoo and hair care product for removing dandruff and relieving itching. The natamycin can effectively inhibit the main fungus malassezia furfur which causes dandruff, has obvious dandruff removing effect, low using dose and is safe and nontoxic. The invention provides a new choice for the development of dandruff removing hair washing and protecting products, can achieve the effects of removing dandruff and relieving itching under the condition of reducing scalp irritation, and has obvious practical value.

Drawings

FIG. 1 is a bacterial colony of natamycin of example 1 against Malassezia furfur;

FIG. 2 is a graph showing drug sensitivity tests of natamycin of example 1 to malassezia furfur, with a visual indication of inhibition of natamycin at different concentrations on the left and OD values of different natamycins at the time of inhibition on the right.

Detailed Description

The invention provides an application of natamycin in preparing a shampoo and hair care product for removing dandruff and relieving itching.

Further, the dosage of the natamycin is more than or equal to 16 mg/mL.

The invention confirms that the natamycin has obvious inhibiting effect on malassezia furfur through a drug sensitive test, and preliminarily infers that the natamycin can be added into a shampoo product as a medicine for removing dandruff and relieving itching. Furthermore, in order to clarify the dosage, the inventor screens the dosage to obtain that the inhibition effect is more obvious when the dosage is more than or equal to 16 mg/mL. The natamycin provided by the invention is used in shampoo products with small dosage, stable effect and safe and nontoxic characteristics, can effectively replace some existing anti-dandruff medicines, and provides a new choice for the development of anti-dandruff products.

The following examples are intended to illustrate specific embodiments of the present invention, but are not intended to limit the scope of the invention to the examples.

Example 1 drug susceptibility test of Natamycin on Malassezia furfur

The specific operation steps are as follows:

1. preparing a culture medium: preparing a liquid culture medium according to the following proportion (1% peptone, 2% -4% glucose, 0.1% yeast powder, 0.4% bovine bile salt, 0.1% glycerol, 0.25% glyceryl monostearate, 0.2% tween 40, 1.5% agar and 2% olive oil), and carrying out autoclaving treatment at 121 ℃ for 15min for later use; a solid medium was prepared by adding 2% agarose to a liquid medium.

2. Activating strains: taking a standard strain (purchased from ATCC, 14521);

the bacterial powder was dissolved in 2mL of the medium, and the resulting solution was added to 50mL of the liquid medium and cultured on a shaker at 220rpm and 28 ℃ for 24 hours.

3. Drug sensitivity test

Taking 0.8mL of malassezia furfur solution in logarithmic phase, uniformly coating the solution in a solid culture medium, adding a sterile Oxford cup, adding 150 mu L of natamycin solution with different concentrations (2, 4, 8 and 16mg/mL) into each hole, placing the mixture in a biochemical incubator at 28 ℃, culturing for 48h, and observing the size of a bacteriostatic colony.

4. Minimum bactericidal concentration detection

A, 0.5mL of logarithmic growth batch malassezia furfur is taken and added into 5mL of fresh liquid culture medium, and the mixture is cultured for 4 hours at 220rpm and 28 ℃.

B, adopting a two-fold dilution method, adding natamycin with different concentrations until the final concentration of the medicine is as follows: 32. 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, 0.0625mg/mL, for 48 h.

And C, measuring the bacterial liquid by using an enzyme-labeling instrument, measuring the OD value at the wavelength of 600nm, and judging the minimum bactericidal concentration.

The experimental results show that: the natamycin has an inhibiting effect on the malassezia furfur, the malassezia furfur has sensitivity to the natamycin, and the natamycin can be used for inhibiting the malassezia furfur and can be applied to preparing a product for removing dandruff and relieving itching.

The natamycin with different concentrations is treated, the inhibition zone of the bran malassezia is increased along with the increase of the drug concentration, the drug concentration dependence is achieved, and the most suitable natamycin sterilization concentration is more than or equal to 16 mg/mL.

The natamycin can effectively inhibit the growth of main fungus bran malassezia furfur for dandruff and has the function of removing dandruff. It was further found that natamycin at low concentration (16mg/mL) was effective in killing Malassezia furfur. The invention provides a powerful basis for preparing the efficient and safe anti-dandruff shampoo, and can effectively solve the trouble of patients with dandruff.

Claims

1. Application of natamycin in preparing shampoo and hair care products for removing dandruff and relieving itching.

2. Use according to claim 1, characterized in that: the hair washing and caring product comprises at least one of shampoo, hair conditioner, hair rinse and hair caring essential oil.

3. Use according to claim 1, characterized in that: the dosage of the natamycin is more than or equal to 16 mg/mL.

4. Use according to claim 1, characterized in that: the natamycin exerts the function of removing dandruff and relieving itching by inhibiting malassezia furfur.

5. Use of natamycin as claimed in claim 1 for inhibiting malassezia furfur.

6. A hair washing and protecting product is characterized in that: comprising natamycin.

7. The hair care shampoo product of claim 6 characterized by: the dosage of the natamycin is more than or equal to 16 mg/mL.

8. The hair care shampoo product of claim 6 characterized by: the product comprises at least one of shampoo, hair conditioner, hair rinse and hair care essential oil.