CN112522103A - Intelligent cell culture dish capable of detecting temperature - Google Patents

Intelligent cell culture dish capable of detecting temperature Download PDF

Info

Publication number
CN112522103A
CN112522103A CN202011556381.3A CN202011556381A CN112522103A CN 112522103 A CN112522103 A CN 112522103A CN 202011556381 A CN202011556381 A CN 202011556381A CN 112522103 A CN112522103 A CN 112522103A
Authority
CN
China
Prior art keywords
cell culture
culture dish
outer side
switching
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202011556381.3A
Other languages
Chinese (zh)
Other versions
CN112522103B (en
Inventor
袁淑静
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Weihai Jiansheng Biotechnology Co ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN202011556381.3A priority Critical patent/CN112522103B/en
Publication of CN112522103A publication Critical patent/CN112522103A/en
Application granted granted Critical
Publication of CN112522103B publication Critical patent/CN112522103B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of cell culture, and discloses an intelligent cell culture dish capable of detecting temperature, which comprises an equipment main body, wherein a driving screw rod is fixedly installed on the outer side of the equipment main body, a switching block is sleeved on the outer side of the driving screw rod, a sub screw rod is movably connected on the outer side of the switching block, one end, far away from the switching block, of the sub screw rod is fixedly connected with a switching shaft center, an L-shaped pipe is fixedly connected on the outer side of the switching shaft center, a circulating pipeline is arranged inside the L-shaped pipe, the top end of the L-shaped pipe is fixedly connected with a suck-back mechanism, and a suck-back fan blade and a suck-back head are arranged. This intelligent cell culture dish of observable temperature through drive screw's rotation, based on the switching of switching piece, the rotation of hold-in range active cell screw rod then drives the rotation in switching axle center to thereby drive the motion downwardly extending of L type pipe, thereby L type pipe drives the resorption mechanism and extends downwards, inserts the inside of cell culture liquid.

Description

Intelligent cell culture dish capable of detecting temperature
Technical Field
The invention relates to the technical field of cell culture, in particular to an intelligent cell culture dish capable of detecting temperature.
Background
A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be of polyethylene material, disposable and reusable, suitable for laboratory inoculation, streaking, bacterial isolation and may be used for the cultivation of plant material.
At present, traditional culture dish can't make the culture dish maintain on certain temperature to some need homothermal cell culture just can't go on, need the intervention of other instruments just can accomplish, not only wastes time and energy, and is still convenient inadequately. Present culture dish can't directly read the inside culture temperature of culture dish when using, only relies on to produce the error easily to the outside temperature measurement of culture dish, can influence the accuracy of experiment. Therefore, we propose an intelligent cell culture dish that can detect temperature.
Disclosure of Invention
Technical scheme
In order to solve the above problems, the present invention provides the following technical solutions: the utility model provides an intelligent cell culture dish of observable temperature, includes equipment principal, equipment principal's outside fixed mounting has drive screw, the switching piece has been cup jointed in drive screw's the outside, the outside swing joint of switching piece has the sub-screw, the one end fixedly connected with switching axle center of switching piece is kept away from to the sub-screw, the outside fixedly connected with L type pipe in switching axle center, the inside of L type pipe is provided with the circulation pipeline, the top fixedly connected with resorption mechanism of L type pipe, the inside of resorption mechanism is provided with the resorption flabellum and the resorption head, the bottom of L type pipe is provided with the mouth that converges, the below fixedly connected with switching water pocket of confluence mouth.
Preferably, one end of the switching water bag, which is far away from the confluence port, is fixedly connected with a temperature measuring tube.
Preferably, the inner side of the temperature measuring tube is provided with scales.
Preferably, a constant temperature tube is fixedly installed on the outer side of the temperature measuring tube.
Preferably, the bottom end of the temperature measuring tube is fixedly provided with a temperature measuring mechanism.
Preferably, the bottom end of the temperature measuring mechanism is fixedly provided with a temperature sensing head.
Preferably, the interior of the device body is provided with a culture dish inner cavity.
Preferably, a cell culture solution is placed inside the inner cavity of the culture dish.
Advantageous effects
Compared with the prior art, the invention provides an intelligent cell culture dish capable of detecting temperature, which has the following beneficial effects:
1. this intelligent cell culture dish of observable temperature through drive screw's rotation, based on the switching of switching piece, the rotation of hold-in range active cell screw rod then drives the rotation in switching axle center to thereby drive the motion downwardly extending of L type pipe, thereby L type pipe drives the resorption mechanism and extends downwards, inserts the inside of cell culture liquid.
2. This intelligence cell culture dish of observable temperature, rotation through the resorption flabellum, thereby produce the resorption wind, thereby the resorption head produces the resorption force, carry out the resorption to cell culture liquid, thereby cell culture liquid is inhaled the inside runner pipe of L type pipe, cell culture liquid passes runner pipe and collection mouth immediately, then get into switching water pocket from the mouth that converges, get into the temperature tube immediately, the temperature of temperature sensing head through the temperature measurement mechanism outside setting is then measured the temperature of cell culture liquid, thereby show the concrete temperature of cell culture liquid on the scale, realize the purpose of temperature measurement.
Drawings
FIG. 1 is a schematic view of the connection structure of the main body of the apparatus of the present invention;
FIG. 2 is a schematic view of a driving screw connection structure according to the present invention;
FIG. 3 is a schematic view of the connection structure of the water transfer bladder of the present invention;
FIG. 4 is a schematic view of the connection structure of the suck back mechanism of the present invention;
FIG. 5 is a schematic view of a connecting structure of a temperature measuring tube according to the present invention;
FIG. 6 is a schematic view of a scale connection structure according to the present invention;
FIG. 7 is a schematic view of a connection structure of the thermal head according to the present invention.
In the figure: 1. an apparatus main body; 2. a drive screw; 3. a transfer block; 4. a sub-screw; 5. transferring the axis; 6. an L-shaped pipe; 7. a flow conduit; 8. a suck-back mechanism; 9. sucking back the fan blades; 10. returning the suction head; 11. a flow converging port; 12. switching the water bag; 13. a temperature measuring tube; 14. calibration; 15. a thermostatic tube; 16. a temperature measuring mechanism; 17. a temperature-sensitive head; 18. an inner cavity of the culture dish; 19. a cell culture solution.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, an intelligent cell culture dish capable of detecting temperature comprises an equipment main body 1, a driving screw 2 is fixedly installed on the outer side of the equipment main body 1, a switching block 3 is sleeved on the outer side of the driving screw 2, a sub-screw 4 is movably connected on the outer side of the switching block 3, one end, far away from the switching block 3, of the sub-screw 4 is fixedly connected with a switching axis 5, an L-shaped pipe 6 is fixedly connected on the outer side of the switching axis 5, and a circulation pipeline 7 is arranged inside the L-shaped pipe 6; through the rotation of drive screw 2, based on the switching of switching piece 3, the rotation of hold-in range active cell screw rod 4 then drives the rotation of switching axle center 5 to the motion downwardly extending of drive L type pipe 6, thereby L type pipe 6 drives suck-back mechanism 8 and extends downwards, inserts the inside of cell culture liquid 19.
The top end of the L-shaped pipe 6 is fixedly connected with a resorption mechanism 8, the resorption mechanism 8 is internally provided with a resorption fan blade 9 and a resorption head 10, the bottom end of the L-shaped pipe 6 is provided with a confluence port 11, and a switching water bag 12 is fixedly connected below the confluence port 11.
One end fixedly connected with temperature tube 13 that switching water pocket 12 kept away from converging mouth 11, the inboard of temperature tube 13 is provided with scale 14, the outside fixed mounting of temperature tube 13 has constant temperature tube 15, the bottom fixed mounting of temperature tube 13 has temperature measurement mechanism 16, the bottom fixed mounting of temperature measurement mechanism 16 has temperature sensing head 17, the inside of equipment main part 1 is provided with culture dish inner chamber 18, cell culture liquid 19 has been placed to the inside of culture dish inner chamber 18.
The back suction fan blade 9 rotates to generate back suction wind, the back suction head 10 generates back suction force to suck back the cell culture solution 19, the cell culture solution 19 is sucked into the circulating pipeline 7 in the L-shaped pipe 6, the cell culture solution 19 immediately passes through the circulating pipeline 7 and the confluence port 11, then enters the switching water sac 12 from the confluence port 11 and immediately enters the temperature measuring pipe 13, the temperature of the cell culture solution 19 is measured through the temperature sensing head 17 arranged on the outer side of the temperature measuring mechanism 16, and therefore the specific temperature of the cell culture solution 19 is displayed on the scale 14, and the purpose of temperature measurement is achieved.
The working principle is as follows: when the cell culture medium transferring device is used, the driving screw 2 rotates, the transferring block 3 is transferred, the synchronous belt rotor screw 4 rotates, the transferring axis 5 is driven to rotate, the L-shaped pipe 6 is driven to move and extend downwards, and the L-shaped pipe 6 drives the suck-back mechanism 8 to extend downwards and insert into the cell culture medium 19; the back suction fan blade 9 rotates to generate back suction wind, the back suction head 10 generates back suction force to suck back the cell culture solution 19, the cell culture solution 19 is sucked into the circulating pipeline 7 in the L-shaped pipe 6, the cell culture solution 19 immediately passes through the circulating pipeline 7 and the confluence port 11, then enters the switching water sac 12 from the confluence port 11 and immediately enters the temperature measuring pipe 13, the temperature of the cell culture solution 19 is measured through the temperature sensing head 17 arranged on the outer side of the temperature measuring mechanism 16, and therefore the specific temperature of the cell culture solution 19 is displayed on the scale 14, and the purpose of temperature measurement is achieved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. The utility model provides an intelligent cell culture dish of observable temperature, includes equipment main part (1), its characterized in that: the outer side of the equipment main body (1) is fixedly provided with a driving screw (2), a transfer block (3) is sleeved on the outer side of the driving screw (2), the outer side of the transfer block (3) is movably connected with a sub-screw (4), one end of the sub-screw (4) is far away from the transfer block (3) and is fixedly connected with a transfer axis (5), the outer side of the transfer axis (5) is fixedly connected with an L-shaped pipe (6), the inside of the L-shaped pipe (6) is provided with a circulation pipeline (7), the top end of the L-shaped pipe (6) is fixedly connected with a resorption mechanism (8), the inside of the resorption mechanism (8) is provided with a resorption fan blade (9) and a resorption head (10), the bottom end of the L-shaped pipe (6) is provided with a confluence opening (11), and the lower side of the confluence opening (11) is fixedly connected with a transfer.
2. The intelligent cell culture dish capable of detecting temperature according to claim 1, wherein: one end of the switching water bag (12) far away from the confluence port (11) is fixedly connected with a temperature measuring tube (13).
3. The intelligent cell culture dish capable of detecting temperature according to claim 2, wherein: scales (14) are arranged on the inner side of the temperature measuring pipe (13).
4. The intelligent cell culture dish capable of detecting temperature according to claim 3, wherein: and a constant temperature pipe (15) is fixedly arranged on the outer side of the temperature measuring pipe (13).
5. The intelligent cell culture dish capable of detecting temperature according to claim 3, wherein: and a temperature measuring mechanism (16) is fixedly arranged at the bottom end of the temperature measuring pipe (13).
6. The intelligent cell culture dish capable of detecting temperature according to claim 5, wherein: and a temperature sensing head (17) is fixedly arranged at the bottom end of the temperature measuring mechanism (16).
7. The intelligent cell culture dish capable of detecting temperature according to claim 1, wherein: the interior of the equipment main body (1) is provided with a culture dish inner cavity (18).
8. The intelligent cell culture dish capable of detecting temperature according to claim 7, wherein: and a cell culture solution (19) is placed in the inner cavity (18) of the culture dish.
CN202011556381.3A 2020-12-25 2020-12-25 Intelligent cell culture dish capable of detecting temperature Active CN112522103B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011556381.3A CN112522103B (en) 2020-12-25 2020-12-25 Intelligent cell culture dish capable of detecting temperature

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011556381.3A CN112522103B (en) 2020-12-25 2020-12-25 Intelligent cell culture dish capable of detecting temperature

Publications (2)

Publication Number Publication Date
CN112522103A true CN112522103A (en) 2021-03-19
CN112522103B CN112522103B (en) 2022-11-29

Family

ID=74976537

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011556381.3A Active CN112522103B (en) 2020-12-25 2020-12-25 Intelligent cell culture dish capable of detecting temperature

Country Status (1)

Country Link
CN (1) CN112522103B (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208055362U (en) * 2018-01-29 2018-11-06 上海奥尚生物科技有限公司 A kind of cell culture apparatus convenient for adjusting culture solution concentration
CN108949563A (en) * 2018-09-14 2018-12-07 刘冀周 A kind of animal and plant cells large-scale culture device
CN209162054U (en) * 2018-10-17 2019-07-26 厦门博创盛世生物技术有限公司 A kind of portable cell culture culture dish
CN209412231U (en) * 2018-12-12 2019-09-20 郭玲 One kind is novel can thermostatic control Tissue Culture Dish
JP2019176815A (en) * 2018-03-30 2019-10-17 ユキエンジニアリング株式会社 Constant temperature conveyance box, conveyance rack used for constant temperature conveyance box, and constant temperature conveyance apparatus combined with the same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208055362U (en) * 2018-01-29 2018-11-06 上海奥尚生物科技有限公司 A kind of cell culture apparatus convenient for adjusting culture solution concentration
JP2019176815A (en) * 2018-03-30 2019-10-17 ユキエンジニアリング株式会社 Constant temperature conveyance box, conveyance rack used for constant temperature conveyance box, and constant temperature conveyance apparatus combined with the same
CN108949563A (en) * 2018-09-14 2018-12-07 刘冀周 A kind of animal and plant cells large-scale culture device
CN209162054U (en) * 2018-10-17 2019-07-26 厦门博创盛世生物技术有限公司 A kind of portable cell culture culture dish
CN209412231U (en) * 2018-12-12 2019-09-20 郭玲 One kind is novel can thermostatic control Tissue Culture Dish

Also Published As

Publication number Publication date
CN112522103B (en) 2022-11-29

Similar Documents

Publication Publication Date Title
CN101985600B (en) Turnover membrane bioreactor for use in micro-gravity environment
CN211665054U (en) A culture apparatus for microbiological examination
ES2914527T3 (en) Parallel bioreactor system
CN102212473B (en) Cell culture system with plate flowing chamber
CN201756550U (en) Fully-sealed bacteria collection ampoule incubator
CN102199537A (en) Membrane bioreactor used in microgravity environment and simulated microgravity environment
CN112522103B (en) Intelligent cell culture dish capable of detecting temperature
CN111218394B (en) Reduce bacterial solution inoculation device that other bacterial sneaked into
CN207567234U (en) A kind of parallel reactor system
CN213232314U (en) Living cell monitoring device
CN206887129U (en) Disposable pneumo lift-type cell culture reactor
CN206014948U (en) A kind of detecting system of cell cycle incubation
CN204509356U (en) Negative pressure constant temperature culture device
CN217628396U (en) Temperature and humidity control strain incubator
CN209684424U (en) A kind of thermometer disinfection storage detection integrated device
CN209098683U (en) A kind of Novel anaerobic fermenting experiment device
CN207958369U (en) A kind of microbial detection device
CN214088532U (en) Incubator for cell culture
CN205662508U (en) Culture apparatus is used in quarantine of food microbial detection
CN207987169U (en) A kind of new bio experiment test piece
CN211005399U (en) Biochemical incubator is used in environmental detection
CN220767020U (en) Constant temperature incubator for blood bacteria detection
CN201634678U (en) Small-sized fermentation device simulating environment of intestinal canal
CN218068028U (en) Single-point velocimeter for fluid
CN215975839U (en) Fermentation equipment capable of automatically filling culture solution

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20221115

Address after: 264203 12th floor, office building of Weihai Zhengsheng Biotechnology Co., Ltd., north of Huanghe street and east of Guangtai airport, Zhangcun Town, Huancui District, Weihai City, Shandong Province

Applicant after: Weihai Jiansheng Biotechnology Co.,Ltd.

Address before: 053000 Hebei Hengshui Taocheng District New District No.7 Road North 50m high creative park

Applicant before: Yuan Shujing

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: An intelligent cell culture dish capable of detecting temperature

Effective date of registration: 20230425

Granted publication date: 20221129

Pledgee: Industrial and Commercial Bank of China Limited Weihai Branch

Pledgor: Weihai Jiansheng Biotechnology Co.,Ltd.

Registration number: Y2023980039089

PE01 Entry into force of the registration of the contract for pledge of patent right