CN112517325A - Glue dripping box, glue dripping device and glue dripping method - Google Patents

Abstract

The invention relates to a glue dripping box, which comprises one or more than one glue dripping injector, an upper fixing plate and a lower fixing plate, wherein the upper fixing plate and the lower fixing plate are clamped on the glue dripping injector; the glue dripping injector comprises a cylindrical injector tube with a cavity and a cylindrical injector inner core; the injector inner core is provided with a cavity for containing liquid glue; the cavity is a conical structure with a large upper part and a small lower part; the injector inner core is positioned in the cavity of the injector tube, the inner wall of the injector tube is provided with a rubber pad, and the inner wall of the injector tube and the rubber pad can slide up and down along the outer wall of the injector inner core. A glue dripping device comprising the glue dripping box and a method for dripping glue on the glass slide by the glue dripping device. The glue dripping of the glass slide can be mechanized, batched and standardized. The glue dripping device is small in structure, the glue dripping method is simple and convenient to operate, and the glue dripping device is particularly suitable for being used in a pathological analysis laboratory or a biological research and development laboratory.

Description

Glue dripping box, glue dripping device and glue dripping method

Technical Field

The invention belongs to the field of medical examination instruments, and relates to a glue dripping box, a glue dripping device and a glue dripping method for carrying out glue dripping treatment on a glass slide.

Background

The pathology department is an important clinical department of medical institutions and medical inspection institutions, bears the task of disease diagnosis, and the pathological diagnosis is an important basis of the disease diagnosis, plays an important role in treatment and prognosis of diseases, directly influences the pathological diagnosis result by the quality of pathological sections, and is a key link and a foundation for clinical pathological quality control.

In order to ensure the quality of pathological sections, the mounting is an important process, and particularly, the mounting means that tissue sections are mounted and stored between a glass slide and a cover glass, so that the tissue sections are not contacted with air, the sections are prevented from being oxidized and faded, and microscopic observation and storage are facilitated.

The specific process of the sealing sheet comprises the following steps: after the mounting glue is dripped on the glass slide with the tissue section, the glass slide is covered by the cover glass.

At present, manual glue dripping is mostly adopted in a pathology laboratory, the glue dripping amount in each time cannot be controlled in a standard way, and if the glue dripping amount is large, liquid overflowing and glass slide plate pollution are generated; if the amount is small, the sealing is incomplete, and the reading and reporting are affected. In addition, the position of the glue dripping is manually controlled, and the glue dripping position is far away from the position of the sample or directly drips on the slide plate, which causes waste and pollution. Moreover, only 1 slide can be dripped with glue for 1 time, which wastes time. Finally, a large amount of glue solution is hung outside the glue dripping pipe, and the glue solution flows down along the suction pipe during glue dripping, so that a large amount of glue solution is left on the glass slide, waste is caused, and the glass slide is adhered.

For the mounting, automatic mounting equipment is gradually developed, and the mounting work is completed by adopting a simulated manual method. The machine is used for sealing, so that the sealing device has the advantages of high sealing efficiency and good quality, and can reduce the physical damage to technicians. However, the existing sheet sealing machine adopts a serial flow line working mode, the glass slides which are manually delivered are placed on a sheet sealing machine flow line for sealing, only one glass slide can be processed at a time, and the sheet sealing machine has the problems of still insufficient working efficiency, long processing time and high labor cost.

Disclosure of Invention

The invention provides a novel glue dripping box, a glue dripping device and a glue dripping method, which can realize mechanization, batch and standardization of glue dripping, are simple and convenient to operate, improve the working efficiency and avoid waste.

The invention provides a glue dripping box which comprises one or more than one glue dripping injector, an upper fixing plate and a lower fixing plate, wherein the upper fixing plate and the lower fixing plate are clamped on the glue dripping injector; the glue dripping injector comprises a cylindrical injector tube with a cavity and a cylindrical injector inner core; the injector inner core is provided with a cavity for containing liquid glue; the cavity is a conical structure with a large upper part and a small lower part; the injector inner core is positioned in the cavity of the injector tube, the inner wall of the injector tube is provided with a rubber pad, and the inner wall of the injector tube and the rubber pad can slide up and down along the outer wall of the injector inner core.

In some preferred embodiments, the glue dripping device further comprises a glue dripping box body formed by enclosing a lower fixing plate and four side walls, and the one or more glue dripping ejectors and the upper fixing plate are located in the glue dripping box body.

In some preferred embodiments, the side wall of the top of the injector tube is provided with an upper annular groove which is concave towards the cavity, and the upper annular groove is clamped and fixed with the upper fixing plate; when the upper annular groove clamps the inner core of the injector, the injector tube cannot move downwards continuously; the side wall of the bottom of the inner core of the injector is provided with a lower annular groove, and the lower annular groove is clamped and fixed with the lower fixing plate.

In some preferred embodiments, when the upper fixing plate moves downwards, the upper fixing plate drives the injector tube to move downwards relative to the injector inner core, and the cavity of the injector inner core is compressed, so that the sealing glue in the cavity is extruded; when the upper fixing plate moves upwards, the injector tube is driven to move upwards relative to the injector inner core, so that the injector tube returns to the original position.

In some preferred embodiments, the glue dripping injector further comprises a cover body, the cover body covers the injector tube, and after the sealing piece glue in the glue dripping injector is used up, the injector tube returns to the original position, the cover body is opened, and the sealing piece glue is injected into the glue dripping injector again.

In some preferred embodiments, a dripper is arranged at the bottom of the inner core of the injector, and the dripper is communicated with the cavity of the inner core of the injector; the dripper extends out of the lower part of the lower fixed plate.

In some preferred embodiments, the outer wall of the inner core of the injector is provided with graduation lines.

In some preferred embodiments, the glue dripping assembly comprises a screw and a nut; the screw vertically penetrates through the upper fixing plate and the bottom of the screw is fixed on the lower fixing plate; the nut is fixed on the upper fixing plate and is spirally connected to the screw rod; the nut is rotated to move downwards along the screw rod, the nut is pressed on the upper fixing plate, the upper fixing plate also moves downwards, and the glue dripping injector clamped on the upper fixing plate is driven to drip glue; and when the sealing piece glue in the glue dripping injector is used up, the nut is rotated in the opposite direction to enable the nut to move upwards along the screw rod, and the upper fixing plate fixedly connected with the nut also moves upwards until the original position is recovered.

In some preferred embodiments, the glue dripping assembly further comprises a control rod with a hollow structure, the control rod is sleeved on the screw rod, and the bottom of the control rod is sleeved on the nut and fixedly connected with the nut; the control rod is rotated to drive the nut to move up and down along the screw rod; and a control disc is arranged at the top of the control rod, and the control rod and the nut are driven to move up and down along the screw rod by rotating the control disc.

Through the cooperation of the glue dripping component and the glue dripping injector, the glue dripping amount is standardized, for example, a rotary nut and a disc matched with the rotary nut are fixed at a certain angle, so that the glue dripping amount is the same every time. For example, the glue is dropped once every 90 degrees is selected, and in some embodiments, the angle may be set to 90 degrees to 360 degrees, such as 90 degrees, 180 degrees, 270 degrees, or 360 degrees. In some preferred schemes, the amount of the glue dripping can be controlled by rotating the control disc for the number of turns to correspond to the rotating angle. In some embodiments, the control disk rotates 0.25 to 2 turns, e.g., 0.25, 0.5, 0.75, 1, 2, etc.

In some preferred embodiments, the number of the glue dispenser is 20, and the glue dispenser is arranged on the upper fixing plate and the lower fixing plate in two rows.

On the other hand, the invention also provides a glue dripping device, which is used for the glue dripping sealing piece of the glass slide and comprises the glue dripping box and a first shell, wherein the glue dripping box is fixed at the top in the first shell, and part of a screw rod, part of a control rod and a control disc of the glue dripping box are arranged outside the first shell; the first shell side wall department of the lower side of the glue dripping box is provided with a first opening, a first sliding rail is arranged in the first opening, and when a glass slide or a glass slide plate is inserted into the first opening, the center of the glass slide is positioned under the glue dripping injector.

In some preferred embodiments, the top of the first casing is provided with another opening, and the other opening is provided with an upper cover.

In some preferred embodiments, a first slide rail is provided in the first opening, and a slide or a tray of slides is inserted into or withdrawn from the first opening through the first slide rail.

The invention also provides a glue dripping method for the slide glass mounting by using the glue dripping device, which comprises the following steps:

(1) placing a glass slide with a sample or a glass slide plate with a plurality of glass slides with samples in a tray, pushing the tray into a first sliding rail, wherein the center of the glass slide is positioned right below a dripper of a glue dripping injector; rotating the control disc to drive the control rod and the nut to rotate downwards, pressing the upper fixing plate downwards to drive the injector tube of the glue dripping injector to move downwards relative to the injector inner core, and compressing a cavity formed by the injector tube and the injector inner core to extrude the sealing glue in the cavity onto the glass slide;

(2) drawing out the tray on which the mounting glue is dripped, and repeating the step (1) to drip the glass slide in the next tray;

(3) repeating the step (2), when the sealing rubber in the rubber dripping injector is used up, the upper annular groove of the injector tube clamps the inner core of the injector, the injector tube cannot move downwards continuously, and the control disc cannot rotate in the same direction any more; rotating the control disc in the opposite direction to drive the control rod and the nut to rotate upwards to drive the upper fixing plate to move upwards and drive the injector tube to move upwards relative to the inner core of the injector so as to restore the injector tube to the original position, opening the upper cover of the first shell and the cover body on the injector tube, and adding the sealing piece glue into the glue dripping injector;

(4) and (3) continuing repeating the steps (1) and (2) and carrying out glue dripping on the slide glass in the next tray.

Advantageous effects

The glue dripping device is matched with the glue dripping injector through the glue dripping component, so that the glue dripping amount is standardized, the glue dripping device can realize the glue dripping of at most 20 glass slides at one time, the work efficiency is greatly improved, the waste of sealing glue of manual operation is reduced, and the problems that the quantity of the manual glue dripping is not standard and the position of the glue dripping position is not standard are solved. The glue dripping of the glass slide can be mechanized, batched and standardized. The glue dripping device has small and exquisite structure and simple and convenient operation, and is particularly suitable for a pathological analysis laboratory or a biological research and development laboratory.

Drawings

FIG. 1 is a schematic view of a glue dispensing apparatus of the present invention;

FIG. 2 is a schematic view of a portion of the glue dispensing box of the glue dispensing apparatus of FIG. 1;

FIG. 3 is another schematic structural diagram of a portion of the glue dispensing box of the glue dispensing apparatus of FIG. 1;

FIG. 4 is another schematic structural diagram of a portion of the glue dispensing box of the glue dispensing apparatus of FIG. 1;

FIG. 5 is a schematic cross-sectional view of a glue dispensing cartridge of the glue dispensing apparatus of FIG. 1;

FIG. 6 is a schematic structural view of a glue dispenser;

FIG. 7 is a schematic structural view of a glue dispenser;

FIG. 8 is a schematic view of a cover glass pick and place device of the present invention;

FIG. 9 is a schematic view of the cover slip picking and placing case of the cover slip picking and placing device of FIG. 8;

FIG. 10 is a schematic view of a cover glass dispenser;

FIG. 11 is a schematic cross-sectional view of a cover glass dispenser;

FIG. 12 is a schematic view of the handle in cooperation with the grip and the cover glass dispenser;

figure 13 is a schematic view of a cover sheet cassette and a portion of the lift mechanism of the cover sheet cassette;

fig. 14 is a schematic view of a portion a of fig. 13 showing a spring assembly during cover of the cassette;

figure 15 is a schematic view of figure 13 at B, where a is a more coverslip in the cover glass cassette and B is a less coverslip in the cover glass cassette;

FIG. 16 is a schematic view of a slide mounting apparatus of the present invention;

FIG. 17 is a schematic view of another state of the slide mounting device of the present invention;

FIG. 18 is a schematic view of a slide plate and slide.

Reference numerals

A slide glass sealing device 1, a housing 10, a first housing 11, a second housing 12, a base 20, a first base 21, a second base 22, a glue dripping device 70, a glue dripping box 71, an upper fixing plate 711, a lower fixing plate 712, a through hole 713, a screw 714, a screw bottom 718, a nut 715, a control rod 716, a control disc 717, a glue dripping injector 72, an injector tube 721, an injector core 722, a rubber pad 723, an upper annular groove 724, a lower annular groove 725, an injector core cavity 726, a dripper 727, an injector tube cover 728, a glue dripping box 73, a cover glass taking and placing device 80, a cover glass box 81, an upper plate 811, a lower plate 812, a taking and placing piece handle 83, a handle cavity 831, an arc-shaped protrusion 832, a handle hand 833, a sheet-shaped protrusion 813, a rotating shaft 814, a 815, a box 845, a groove 817, a hole 818, a cover glass taking and placing device 85, a piston barrel 851, an air plug 852, a spring support column 853, a support spring 854, a movable cavity 855, a rubber washer 856, a rubber gasket 857, a first annular groove 858, a second annular groove 859, a cover glass cassette 82, a cover glass placement area 821, a cover glass frame 822, a support rod 823, a support spring 824, a cover glass cassette groove 826, a cover glass placement area bottom 827, a traction wire 861, a first wire 862, a second wire 863, a fixed pulley 864, 866, 842, a hanging bar 865, a handle 84, a spring clamp 841, a first wire access port 843, a second wire access port 844, a handle grip 846, a spring assembly 87, a spring post 871, a spring sleeve 872, a stop 873, a cover glass cassette top 825, a tray 30, a slide plate 40, a slide area 41, a slide 50, a cover glass 60, a first slide rail 112, a second slide rail 122, a first opening 111, a second opening 121, a third opening 124, a support tab 123, and a cover glass 113 on the first housing.

Detailed Description

In the following detailed description, reference is made to the accompanying drawings that form a part hereof, and in which is shown by way of illustration specific embodiments in which the invention may be practiced. It is not excluded that the invention can also be implemented in other embodiments and that the structure of the invention can be varied without departing from the scope of use of the invention.

As shown in fig. 1 to 7, the dispensing device 70 of the present invention for dispensing a slide 50 includes a dispensing box 71 and a first housing 11, wherein the dispensing box 71 is fixed on the top inside the first housing 11. For example, in some embodiments, a bracket or a supporting strip located at the bottom of the glue dripping box 71 is disposed on the inner side wall of the first housing 11, and the glue dripping box 71 is located on the bracket or the supporting strip. As shown in fig. 1, a first opening 111 is provided at a side wall of the first housing 11 below the dispensing cassette 71, and when the slide 50 or the slide plate 40 is inserted into the first opening 111, the slide 50 is centered directly below the dispensing pusher 72 of the dispensing cassette. The first opening 111 is provided with a first slide rail 112 therein, and the tray 30 on which the slide or the slide is placed is inserted into or drawn out of the first opening 111 through the first slide rail 112, and usually, the first opening 111 is provided on the front side wall of the first housing 11 for easy operation.

In the present invention, the glue dripping box 71 includes one or more glue dripping injectors 72, an upper fixing plate 711, and a lower fixing plate 712. In one specific embodiment, the dispensing box 71 further includes a dispensing box 73 surrounded by a lower fixing plate 712 and four sidewalls, and the dispensing injector 72 and the upper fixing plate 711 are located in the dispensing box 73, as shown in fig. 5. The glue dispenser 72 is engaged with and fixed to the upper fixing plate 711 and the lower fixing plate 712. As shown in fig. 6 and 7, the drip injector 72 includes a cylindrical injector tube 721 having a cavity and a cylindrical injector core 722; the injector inner core 722 is positioned in the cavity of the injector tube 721, the rubber pad 723 is arranged on the inner wall of the injector tube 721, and the inner wall of the injector tube 721 and the rubber pad 723 can slide up and down along the outer wall of the injector inner core 722. Injector core 722 has a cavity that contains liquid mounting adhesive; wherein, the cavity is a conical structure with a big top and a small bottom. The conical cavity is arranged to enable the sealing rubber to be extruded and dripped out as much as possible, so that the waste of the sealing rubber is avoided. When the injector tube moves up and down along the injector core, the injector tube cavity and the injector core cavity form a sealed variable space, and the rubber gasket 723 seals the space, thereby realizing extrusion dripping of the sealing rubber in the space. An upper annular groove 724 recessed towards the cavity is formed in the side wall of the top of the injector tube 721, and the upper annular groove 724 is fixedly clamped with the upper fixing plate 711; specifically, the upper fixture is provided with a through hole 723 which is matched with (identical to or slightly larger than) the outer diameter of the upper annular groove, and the upper annular groove is sleeved with the through hole. Since the upper annular groove is recessed into the cavity of the syringe plunger, when the upper annular groove 724 runs on the top of the syringe plunger 722, the syringe plunger is stuck, and the syringe plunger 721 cannot move downwards continuously, which indicates that the sealing rubber in the syringe 72 is used up. The bottom side wall of the plunger core 722 is provided with a lower annular groove 725, and the lower annular groove 725 is fastened and fixed with the lower fixing plate 712. When the upper fixing plate moves downwards, the injector tube is driven to move downwards relative to the injector inner core, and the cavity of the injector inner core is compressed, so that the sealing rubber in the cavity is extruded; when the upper fixing plate moves upwards, the injector tube is driven to move upwards relative to the injector inner core, so that the injector tube returns to the original position. The glue dripping injector 72 further comprises a cover body 728 which covers the injector tube 721, and when the sealing rubber in the glue dripping injector 72 is used up, the injector tube 721 returns to the original position, the cover body 728 is opened, and the sealing rubber is injected into the glue dripping injector 72 again. In some embodiments, the bottom of the syringe inner core 722 is provided with a dropper 727, the dropper 727 is communicated with the syringe inner core cavity, and the dropper 727 extends out of the lower fixing plate 712. In order to add the sealing piece glue to the glue dripping injector conveniently, the top of the first shell 11 is provided with another opening, and the opening position corresponds to the glue dripping injector in the delivery box; the other opening is provided with an upper cover 113.

As shown in fig. 18, the conventional slide plate 40 is provided with 20 slide areas 41, which are divided into two rows of 10 slides, and the slide 50 is placed in the slide area 41. To correspond to the slide 50 on the conventional slide plate 40, in some embodiments, the glue dispenser 72 is provided in 20 rows of 10 glue dispensers arranged in two rows on the upper and lower mounting plates.

The glue dripping box 71 further comprises a glue dripping assembly connected with the upper and lower fixing plates. Specifically, as shown in fig. 3 and 4, the glue dripping assembly includes a screw 714 and a nut 715. The bottom of the screw 714 is fixed to the lower fixing plate 712, and in one embodiment, the screw has a base 718, which is located at the lower portion of the lower fixing plate, and the screw passes through the lower fixing plate 712, and the base and the lower fixing plate can be locked and fixed by bolts. The screw 714 vertically passes through the upper fixing plate 711, and the upper fixing plate can slide up and down relative to the screw. After the nut 715 is screwed onto the threaded rod 714, the nut 715 is fixed to the upper fixing plate, for example, by welding, gluing, or the like. The nut 715 is rotated to move the nut 715 downwards along the screw 714, the nut is pressed on the upper fixing plate 711, the upper fixing plate also moves downwards to drive the injector tube 721 of the glue dripping injector 72 clamped on the upper fixing plate to move downwards, so that the space formed by the cavity of the injector tube and the cavity of the inner core of the injector is reduced, the sealing glue in the space is extruded out, and the glue dripping is carried out; that is, when the sealing rubber in the rubber dropping injector 72 is used up, the nut 715 is rotated in the opposite direction to move the nut upward along the screw 714, and the upper fixing plate 711 fixedly connected with the nut also moves upward to drive the injector tube clamped on the upper fixing plate to move upward until the original position is recovered. The glue dripping component also comprises a control rod 716 with a hollow structure, the control rod 716 is sleeved on the screw 714, and the bottom of the control rod 716 is sleeved on the nut 715 and is fixedly connected with the nut 715; the control rod 716 is rotated to drive the nut 715 to move up and down along the screw 714; the control rod 716 may also be provided with a control disc 717 at the top, and the control rod 716 and the nut 715 are moved up and down along the screw 714 by rotating the control disc 717, as shown in fig. 2. The amount of the glue dripping is controlled by rotating the number of turns of the control disc to correspond to the rotating angle. In some embodiments, the control disk rotates 0.25 to 2 turns, e.g., 0.25, 0.5, 0.75, 1, 2, etc. For ease of operation, a portion of the screw 714 and a portion of the control rod 716 and the control disk 717 are disposed outside the first housing 11.

The glue dripping device 70 is used for dripping glue, and the concrete description is as follows:

(1) placing a slide glass with a sample or a slide glass plate with a plurality of slide glasses with a sample in the tray 30, pushing the tray 30 into the first slide rail 112, wherein the center of the slide glass 50 is positioned under the dripper 727 of the glue dripping injector (when the slide glass is a plurality of slide glasses and positioned on the slide glass plate, the corresponding glue dripping injectors are also in corresponding quantity and arrangement mode, and the slide glasses and the drip injector are in one-to-one correspondence); the control disc 717 is rotated for a certain number of turns (for example, 0.25-2 turns) to drive the control rod 716 and the nut 715 to rotate downwards, the upper fixing plate 711 is pressed downwards to drive the injector tube 721 of the glue dripping injector to move downwards relative to the injector inner core 722, and a cavity formed by the injector tube and the injector inner core is compressed, so that the sealing glue in the cavity is extruded onto the slide 50;

(2) drawing out the tray 30 dripped with the mounting glue, and repeating the step (1) to drip the glass slide 50 in the next tray;

(3) repeating the step (2), when the sealing rubber in the rubber dripping injector is used up, the upper annular groove of the injector tube blocks the inner core of the injector, the injector tube cannot move downwards continuously, and the control disc 717 cannot rotate in the same direction any more; rotating the control disc in the opposite direction to drive the control rod and the nut to rotate upwards to drive the upper fixing plate to move upwards and drive the injector tube to move upwards relative to the inner core of the injector so as to restore the injector tube to the original position, opening the upper cover 113 of the first shell and the cover body 728 on the injector tube, and adding the mounting adhesive into the adhesive dripping injector;

(4) and (3) continuing repeating the steps (1) and (2) and carrying out glue dripping on the slide glass in the next tray.

As shown in fig. 8-15, a cover glass taking and placing device 80 of the present invention comprises a cover glass taking and placing box 81, a cover glass box 82 and a lifting mechanism of the cover glass box 82, and a second housing 12 for accommodating the cover glass taking and placing box 81, the cover glass box 82 and the lifting mechanism, wherein the cover glass taking and placing box 81 is fixed on the top inside the second housing 12, and in one embodiment, a bracket or a supporting strip is arranged on the inner side wall of the second housing, and the cover glass taking and placing box is arranged on the bracket or the supporting strip. A cover sheet cassette 82 is disposed at the bottom of the second housing 12. A second opening 121 is provided in a side wall of the second housing, and typically, the second opening 121 is provided in a front side surface of the second housing 12; and, the second opening 121 is located between the cover glass taking and placing cassette 81 and the cover glass cassette 82. When the slide glass plate or the slide glass plate is inserted into the second opening, the slide glass is positioned under the cover glass taking and placing box 8 and corresponds to the cover glass taking and placing position of the cover glass taking and placing box. The second opening is provided with a second slide rail 122 on the inner side wall of the second housing for matching with the second opening to draw the tray with the slide or slide plate, and in some embodiments, in order to ensure the support of the tray, a support piece 123 is further provided at the second opening 121, as shown in fig. 8.

The cover glass taking and placing box 81 of the present invention includes one or more cover glass taking and placing units 85, and an upper plate 811 and a lower plate 812 which are engaged with the cover glass taking and placing units 85, as shown in fig. 9. The cover slip picking and placing box 81 further comprises a box body 816 enclosed by a lower plate 812 and four side walls, wherein the cover slip picking and placing device 85 and the upper plate 811 are positioned in the box body 816. The cover glass taking and placing box 81 also comprises a handle 83, one end of the handle 83 is connected with one end of the box body 816 through a rotating shaft 815, and the handle can rotate relative to the rotating shaft 815 and the connecting end of the box body so as to realize the downward pressing and upward lifting of the handle. The handle 83 is movably connected to the upper plate 811, and specifically, the handle 83 includes an arc-shaped protrusion 832, and the highest point of the arc-shaped protrusion 832 is in surface contact with the upper plate 811. The upper plate 811 is provided with two symmetrical sheet-shaped protrusions 813, the rotating shaft 814 is located above the handle, and two ends of the rotating shaft 814 are respectively fixedly connected to the two sheet-shaped protrusions 813. The shaft 814 and the plate-like protrusion 813 function as a stopper, that is, the handle 83 is located between the shaft 814 and the upper plate 811. In a specific embodiment, the rotating shaft 814 is located above the arc-shaped protrusion 832 and is used for limiting the arc-shaped protrusion of the handle, when the handle is pressed down, the arc-shaped protrusion contacts the surface of the upper plate, and pressure is applied to make the upper plate move downwards; after the handle is lifted up, the arc-shaped bulge leaves the upper plate due to a certain gap between the rotating shaft and the arc-shaped bulge, and the upper plate rises to return to the original position under the elastic action of the cover glass taking and placing device after losing pressure. In some embodiments, the height of the gap between the rotating shaft and the arc-shaped protrusion is the same as the length of the movable cavity of the cover glass taking and placing device. In some embodiments, the side wall of the case 816 opposite the side wall to which the handle 83 is attached has a recess 817 for receiving the handle and allowing the handle grip 833 to extend out of the case 816. The handle 84 is lifted up or pressed down to drive the upper plate 811 and the cover glass extractor 85 clamped on the upper plate 811 to move, so that the cover glass extractor 85 can take and place the cover glass. In some embodiments, the handle and the grip portion of the grip are located outside the second housing, and in particular, above the second opening of the second housing, there is a third opening 124 through which the handle and the grip portion of the grip protrude from the second housing.

As shown in fig. 10 and 11, the cover glass dispenser 85 includes a piston cylinder 851 and an air plug 852, and the air plug 852 is located inside the piston cylinder 851. The air plug 852 is provided with two symmetrical spring support posts 853, and the support spring 854 is sleeved on the spring support posts 853. The wall of the piston barrel 851 is provided with a movable cavity 855 for the movement of the spring support column, one end of the spring support column 853 is located in the cavity 855 of the wall of the piston barrel, and the spring support column 853 can move up and down in the movable cavity 855. The bottom of the cylinder 851 is provided with a rubber washer 856. The bottom of the air plug 852 is provided with a rubber gasket 857. A first annular groove 858 is arranged at the top of the air plug 852, the first annular groove 858 is fixedly clamped with the upper plate 811, and specifically, a hole 818 matched with the first annular groove is arranged on the upper plate; the hole 818 on the upper plate is sleeved on the first annular groove, so that the upper plate is clamped with the first annular groove. The middle or lower portion of the sidewall of the piston cylinder is provided with a second annular groove recessed into the cavity of the piston cylinder, and the second annular groove 859 is engaged and fixed with the lower plate 812, and similarly, is engaged and fixed through a hole (not shown) provided on the lower plate and adapted to the second annular groove. Specifically, the movable cavity 855 in the barrel is positioned above the second annular recess 859. Because the second annular groove is sunken towards the inner part of the piston cylinder cavity, when the air plug descends to the second annular groove, the air plug is blocked, and the air plug is prevented from continuously moving downwards.

The cover glass taking and placing device 80 lifts the cover glass box 82 to the bottom of the cover glass taking and placing box 81 through the lifting mechanism, and the cover glass box 82 is moved to the original position through the lifting mechanism in cooperation with the cover glass taking and placing box 81. Specifically, the lift mechanism includes a plurality of wires, a resilient member on the handle 83, and a pulley assembly. Wherein, a plurality of steel wires connect the cover glass box 82, the elastic component and the pulley component, and the cover glass box 82 is pulled by the steel wires to ascend and descend by the cooperation of the elastic component and the pulley component. Wherein the resilient member includes a spring clip 841, a fixed pulley 842, and a grip 84, as shown in fig. 12. One end of the handle 84 is rotatably connected to a handle grip 833 protruding from the case 816 through a shaft 845. A cavity 831 is arranged in the handle 83, a traction steel wire 861 is arranged in the cavity 831, one end of the traction steel wire 861 is connected to the grab handle 84 after being turned by a fixed pulley 842 fixed in the cavity, a first steel wire access port 843 and a second steel wire access port 844 are arranged in the cavity, and the first steel wire access port 843 and the second steel wire access port 844 are respectively arranged on the handle cavities at two corresponding side walls of the box body 816. A spring clip 841 is secured between the handle 83 and the grip 84 for maintaining elastic tension between the handle and the grip. The pulley assembly includes fixed pulleys 864 fixed at the four top corners of the housing 816 and fixed pulleys located at the four bottom corners of the cover cassette 82. In one embodiment, two suspension rods 865 are provided at the top of the enclosure 816 or the top interior of the second housing 12, the suspension rods 865 being fixed at the top symmetrical edge of the enclosure or the second housing; four fixed pulleys 864 are fixed to both ends of the suspension lever 865, respectively, as shown in fig. 13. The first steel wire 862 is connected to the two fixed pulleys 864 at one side of the box 816 and the two fixed pulleys 866 at the same side of the glass box 82, and then is connected to the traction steel wire 861 through the first steel wire access port 843; the second wire 863 is connected to the two fixed pulleys 864 at the other side of the box 816 and the two fixed pulleys 866 at the same side of the cover slide cassette, and then connected to the traction wire 861 through the second wire inlet 844.

The lift mechanism further includes six sets of spring assemblies 87 that respectively secure the four corners and the middle of the two sides of the bottom of the cover glass cassette 82 and at the same time secure the bottom of the cassette. As shown in fig. 14, the spring assembly 87 includes a spring post 871 and a spring cover 872 fitted over the spring post; a stop piece 873 is arranged at the lower part of the spring column 817 and is fixed on the second shell, and a certain space is formed between the stop piece and the bottom of the shell. The spring post passes through the stop 873 and can move up and down relative to the stop, and the spring housing 872 is located between the stop 873 and the bottom of the cover cassette 83. In one embodiment, the spring pocket 872 and portions of the spring posts 871 are located in the cover glass cassette 82, i.e., four corners and two side midpoints of the bottom of the cover glass cassette are provided with a cover glass cassette recess 826 from bottom to top, the spring pocket 872 and portions of the spring posts 871 are located in the cover glass cassette recess 826, and the spring pocket 872 contacts the cover glass cassette top 825. After spring sheath 872 is compressed, spring post 871 descends until the bottom of cover slide cassette 82 rests on stop 873; when the pressure is removed, the spring sheath 872 is released to a stretched state, causing the spring 871 to move upward and move upward together with the cover glass cassette 82.

As shown in fig. 12, 13 and 14, when the handle 84 is under the action of the spring clip 841, the handle grip end 846 thereof is away from the handle grip end 833, the pull wire 861 attached to the handle is tensioned, and the second wire 862 and the third wire 863 attached thereto are also tensioned, transmitted through the fixed pulleys 864, 866, to generate a downward pulling force on the slide cassette 82, which counteracts the elastic force of the spring assembly 87 located at the bottom of the slide cassette 82, to keep the slide cassette 82 in place; when the handle 84 is gripped, the spring force of the spring clip 841 is compressed, the traction wire and the first and second wires are in a relaxed state, no tension is generated to the slide cassette 82, and the slide cassette 82 is pushed upward by the spring force of the spring assembly 87, at this time, the state of the spring assembly is shown in fig. 14.

The cover glass cassette 82 includes one or more cover glass placement areas 821, each cover glass placement area 821 being provided with a cover glass frame 822, a support rod 823 at the bottom of the cover glass frame, and a support spring 824 sleeved on the support rod, as shown in fig. 15. The support rod passes through the cover glass placing section bottom 827, and the support rod is movable up and down with respect to the cover glass placing section bottom 827. The support springs 824 are located between the bottom of the cover slip frame 822 and the bottom 827 of the cover slip placement area. That is, the cover slip frame 822 for loading the cover slip 60 is located on the support rods and the support springs, and when more cover slips are loaded in the cover slip frame, the support springs are compressed, and the support rods are mostly below the bottom 827 of the cover slip placement area, at which time the top of the cover slip frame is a greater distance from the cover slip placement area 821, as shown in A of FIG. 15; when the cover slips in the cover slip frame are reduced, the weight is reduced, the supporting springs are compressed less, the elastic force is increased, the cover slip frame is driven to ascend, the supporting columns ascend relative to the bottom 827 of the cover slip placing area, and the distance from the top of the cover slip frame to the cover slip placing area 821 is smaller at the moment, as shown in fig. 15B. In this way, the uppermost cover glass of the cover glass placing area 821 can be kept at the same height, which provides convenience for cooperating with the cover glass taking device of the cover glass taking and placing device to take the cover glass. That is, after the cover slips located in the cover slip frame are taken away one by one, the support spring gradually rises due to the elastic force, so that the uppermost cover slip is always kept at the same height in the cover slip frame, and the cover slip in the cover slip box is tightly attached to the bottom rubber gasket of the piston cylinder of the cover slip taking and placing device, as shown in fig. 15 a and B. In some embodiments, the cover glass cassette has 20 cover glass receiving areas arranged in two rows of 10 slides each.

The method for taking and placing the cover glass and covering the glass slide by the cover glass taking and placing device is described as follows:

1) in an initial state, the cover glass box is positioned at the bottom in the second shell, and the cover glass taking and placing box is positioned at the top in the second shell; the handle and the grab handle are both in a natural drooping and loose state; the traction steel wire connected with the grab handle and the first and second steel wires connected with the grab handle are tensioned, and the traction force generated by the first steel wire and the second steel wire acts on the cover glass box and keeps balance with the spring assembly at the bottom of the cover glass; the bulge of the handle is pressed on the upper plate, and the air plug of the cover glass taking and placing device which is clamped with the upper plate is pressed in the piston cylinder;

2) after an operator holds the holding part of the handle and the holding part of the grab handle tightly, the holding part of the grab handle is superposed with the holding end of the handle, the spring clamp is compressed, the grab handle is lifted up, the traction steel wire is in a loose state, the first steel wire and the second steel wire connected with the traction steel wire are also in a loose state, the first steel wire and the second steel wire generate traction force which is transmitted to the cover glass box through the fixed pulley and descend, the cover glass box is lifted to the bottom of the cover glass taking and placing box under the action of the spring assembly, and the cover glass in the cover glass box is enabled to be tightly attached to the bottom rubber pad of the piston cylinder of the cover glass taking and placing; when the cover slips in the cover slip frame are taken away one by one, the supporting spring gradually rises due to the elastic force, the cover slip frame is lifted to rise, the uppermost cover slip is always kept at the same height in the cover slip placing area, and the cover slip in the cover slip box is tightly attached to the rubber gasket at the bottom of the piston cylinder of the cover slip taking and placing device;

3) lifting the handle and the grab handle under the condition of keeping the coincidence state of the grab handle and the hand-held end of the handle, wherein the handle is protruded to leave the upper plate, the pressure of the upper plate disappears, the upper plate and the air plug clamped and fixed on the upper plate move upwards under the driving of the resilience of the supporting spring, so that the volume of a closed space formed by the piston cylinder, the rubber gasket, the air plug, the rubber gasket and the cover glass is increased, the air pressure in the space is reduced, and the cover glass is adsorbed at the bottom of the piston cylinder; completing the film taking;

4) when the handle and the grab handle are kept in a lifting state, the grab handle is loosened, the spring clamp between the grab handle and the handle pushes the grab handle away from the handle, the traction steel wire connected with the grab handle is tensioned to drive the first steel wire and the second steel wire to be tightened, and after passing through the fixed pulley, the downward traction force of the first steel wire and the second steel wire is increased to drive the cover glass box to move downwards to the bottom of the cavity;

5) pushing a tray with the glass slide or the glass slide plate with the glass slide finished by glue dripping into a space between the cover glass box and the cover glass taking and placing box through a second opening and a second sliding rail, so that the glass slide is positioned under the cover glass adsorbed by the cover glass taking and placing device;

6) pressing the handle to an initial state, raising the lower upper plate on the handle, driving the air plug clamped on the upper plate to move downwards, reducing the volume of a closed space formed by the piston cylinder, the rubber gasket, the air plug, the rubber gasket and the cover glass to zero, so that the air pressure in the space is reduced to enable the air pressure in the piston cylinder to rise to an atmospheric pressure value, releasing the cover glass originally adsorbed at the bottom of the piston cylinder, and covering the cover glass on the corresponding glass slide subjected to glue dripping to finish the sealing;

7) drawing out the tray after the sealing sheet; and repeating the steps 1) -6) to finish the sealing piece of the next tray.

As shown in fig. 16 and 17, the slide glass mounting device 1 of the present invention is integrally composed of a glue dripping device 70 and a cover glass taking and placing device 80. The first housing and the second housing are combined into a single housing 10, and the first housing and the second housing may be arranged up and down or left and right. When the first shell and the second shell are arranged up and down, the first shell is positioned above the second shell, so that the upper cover of the first shell can be conveniently opened.

In one embodiment, the slide plate 40 has 20 slide zones 41 arranged in two columns for receiving 20 slides 50; the glue dripping device 70 is provided with 20 glue dripping pushers 72 which are arranged in the glue dripping box 71 in two rows, namely, the positions of the glue dripping pushers 72 correspond to the positions of the glass slides 50 of the glass slide plate arranged in the first opening 111 one by one; the cover slip taking and placing device 80 is provided with 20 cover slip taking and placing devices 85 which are arranged in the cover slip taking and placing box 81 in two rows, wherein the positions of the cover slip taking and placing devices 85 correspond to the positions of the slide slides 50 of the slide glass plate arranged in the second opening 121 one by one.

When the slide glass mounting device 1 is used for mounting a slide glass, the specific steps are as follows:

(1) placing a sample slide 50 or a slide plate 40 having a plurality of sample slides in the tray 30, pushing the tray 30 into the first opening 111 such that the center of the slide 50 is located directly below the drip emitter drip 727; the control disc 717 is rotated for a certain number of turns or degrees to drive the control rod 716 and the nut 715 to rotate downwards, the upper fixing plate 711 is pressed downwards to drive the injector tube 721 of the glue dripping injector to move downwards relative to the injector inner core 722, and a cavity formed by the injector tube and the injector inner core is compressed to extrude the mounting glue in the cavity onto the glass slide to finish one-time glue injection; as shown in fig. 16;

(2) after the hand holding portion 833 of the handle 83 and the hand holding portion 846 of the handle are gripped, the hand holding portion of the handle is overlapped with the hand holding end of the handle, the spring clamp 841 is compressed, the handle 84 is lifted, the traction steel wire 861 becomes a loose state, the first and second steel wires 862 and 863 connected with the handle also become a loose state, the traction force generated by the first and second steel wires and transmitted to the cover glass box 82 through the fixed pulleys 864 and 866 is reduced, the cover glass box 82 is lifted to the bottom of the cover glass taking and placing box 81 under the action of the spring assembly 87, so that the cover glass 60 in the cover glass box is tightly attached to the bottom rubber washer 856 of the piston cylinder of the cover glass taking and placing device;

(3) lifting the handle 83 and the grab handle 84 when the grab handle is kept in a state of being overlapped with the hand-held end of the handle, enabling the handle bulge 832 to be separated from the upper plate, the pressure of the upper plate disappears, the upper plate and the air plug 852 clamped and fixed on the upper plate move upwards under the driving of the resilience of the supporting spring 854, so that the volume of a closed space formed by the piston cylinder 851, the rubber washer 856, the air plug 852, the rubber gasket 857 and the cover glass 60 is increased, the air pressure in the space is reduced, and the cover glass 60 is adsorbed at the bottom of the piston cylinder 851; completing the film taking;

(4) when the handle and the grab handle are kept in a lifting state, the grab handle 84 is loosened, the spring clamp 841 between the grab handle and the handle pushes the grab handle away from the handle, the traction steel wire 861 connected with the grab handle is tensioned to drive the first steel wire 862 and the second steel wire 863 to be tightened, and after the fixed pulleys 864 and 866, the downward traction force of the first steel wire 862 and the second steel wire 863 is increased to drive the cover glass box 82 to move downwards to the bottom of the cavity until the traction force is balanced with the elastic force of the spring assembly;

(5) the tray 30 to which the mounting adhesive is added is drawn out from the first opening 111 of the first housing 11 and inserted into the second opening 121 of the second housing 12 in parallel, so that the tray 30 is positioned between the cover glass box 82 and the cover glass taking and placing box 81, and the slide 50 is positioned right below the cover glass 60 adsorbed by the cover glass taking and placing device 85; meanwhile, another slide with sample 50 or a plurality of slide plates with sample 40 can be inserted into the first opening of the first shell and placed on the tray 30, and the step (1) can be repeated to perform the glue dripping operation; as shown in fig. 17;

(6) the handle 84 is pressed down to an initial state, the handle upper bulge 832 presses the upper plate 811 down to drive the air plug 852 clamped on the upper plate to move downwards, the volume of a closed space formed by the piston cylinder 851, the rubber washer 856, the air plug 852, the rubber gasket 857 and the cover glass 60 is reduced to a space in the initial state, the air pressure in the space is increased to an atmospheric pressure value, the cover glass 60 originally adsorbed at the bottom of the piston cylinder 851 is released, and the cover glass 60 covers the corresponding glass slide 50 subjected to glue dripping to complete the sealing;

(7) the tray 30 after the cover sheet is drawn out; and (5) repeating the steps (1) to (6) to finish the sealing piece of the next tray.

In the step (1), when the sealing rubber in the rubber dripping injector 72 is used up, the upper annular groove of the injector tube blocks the inner core of the injector, the injector tube cannot move downwards continuously, and the control disc cannot rotate in the same direction any more; and rotating the control disc in the opposite direction to drive the control rod and the nut to rotate upwards to drive the upper fixing plate to move upwards and drive the injector tube to move upwards relative to the inner core of the injector so as to restore the injector tube to the original position, opening the upper cover of the other opening positioned above the first shell and the cover body positioned on the injector tube, and adding the sealing sheet glue into the glue dripping injector.

In step (3), after the cover slips 60 in the cover slip frame are removed one by one, the supporting spring 824 is gradually raised by the elastic force, so that the uppermost cover slip 60 is always kept at the same height in the cover slip placing area 821, and the cover slip 60 in the cover slip case is always tightly attached to the bottom rubber washer 856 of the plunger of the cover slip removing device, as shown in fig. 15.

Claims (10)

1. A glue dripping box is characterized by comprising one or more glue dripping ejectors, an upper fixing plate and a lower fixing plate which are clamped on the glue dripping ejectors, and a glue dripping assembly connected with the upper fixing plate and the lower fixing plate; the glue dripping injector comprises a cylindrical injector tube with a cavity and a cylindrical injector inner core; the injector inner core is provided with a cavity for containing liquid glue; the cavity is a conical structure with a large upper part and a small lower part; the injector inner core is positioned in the cavity of the injector tube, the inner wall of the injector tube is provided with a rubber pad, and the inner wall of the injector tube and the rubber pad can slide up and down along the outer wall of the injector inner core.

2. The glue dripping box according to claim 1, wherein the side wall of the top of the injector tube is provided with an upper annular groove which is concave towards the cavity, and the upper annular groove is clamped and fixed with the upper fixing plate; when the upper annular groove clamps the inner core of the injector, the injector tube cannot move downwards continuously; the side wall of the bottom of the inner core of the injector is provided with a lower annular groove, and the lower annular groove is clamped and fixed with the lower fixing plate.

3. The glue dripping box according to claim 2, wherein when the upper fixing plate moves downwards, the upper fixing plate drives the injector tube to move downwards relative to the injector inner core, and the cavity of the injector inner core is compressed, so that the sealing glue in the cavity is extruded; when the upper fixing plate moves upwards, the injector tube is driven to move upwards relative to the injector inner core, so that the injector tube returns to the original position.

4. The glue dripping box according to claim 2, wherein the glue dripping injector further comprises a cover body, the cover body covers the injector tube, and when the sealing piece glue in the glue dripping injector is used up, the injector tube returns to the original position, the cover body is opened, and the sealing piece glue is injected into the glue dripping injector again; the bottom of the injector inner core is provided with a water dropper, and the water dropper is communicated with the cavity of the injector inner core; the dripper extends out of the lower part of the lower fixed plate.

5. The dispensing cartridge according to any of claims 1-4, wherein the dispensing assembly comprises a threaded rod and a nut; the screw vertically penetrates through the upper fixing plate and the bottom of the screw is fixed on the lower fixing plate; the nut is fixed on the upper fixing plate and is spirally connected to the screw rod; the nut is rotated to move downwards along the screw rod, the nut is pressed on the upper fixing plate, the upper fixing plate also moves downwards, and the glue dripping injector clamped on the upper fixing plate is driven to drip glue; and when the sealing piece glue in the glue dripping injector is used up, the nut is rotated in the opposite direction to enable the nut to move upwards along the screw rod, and the upper fixing plate fixedly connected with the nut also moves upwards until the original position is recovered.

6. The dispensing box according to claim 5, wherein the dispensing assembly further comprises a control rod with a hollow structure, the control rod is sleeved on the screw rod, and the bottom of the control rod is sleeved on the nut and is fixedly connected with the nut; the control rod is rotated to drive the nut to move up and down along the screw rod; and a control disc is arranged at the top of the control rod, and the control rod and the nut are driven to move up and down along the screw rod by rotating the control disc.

7. The paste dispenser according to claim 1, wherein the paste dispenser is 20 in number, and is arranged in two rows on the upper fixing plate and the lower fixing plate.

8. A glue dripping device for a glue dripping sealing sheet of a glass slide, which is characterized by comprising a glue dripping box as claimed in any one of claims 1 to 7 and a first shell, wherein the glue dripping box is fixed at the top in the first shell, and a part of screw rods and a part of control rods and a control disc of the glue dripping box are arranged outside the first shell; the first shell side wall department of the lower side of the glue dripping box is provided with a first opening, a first sliding rail is arranged in the first opening, and when a glass slide or a glass slide plate is inserted into the first opening, the center of the glass slide is positioned under the glue dripping injector.

9. The glue dripping device according to claim 8, wherein the first housing has a further opening at the top, and the further opening is provided with a cover.

10. A method for dispensing a slide mount, comprising the dispensing apparatus of claim 8 or 9, comprising the steps of:

(1) placing a glass slide with a sample or a glass slide plate with a plurality of glass slides with samples in a tray, pushing the tray into a first sliding rail, wherein the center of the glass slide is positioned right below a dripper of a glue dripping injector; rotating the control disc to drive the control rod and the nut to rotate downwards, pressing the upper fixing plate downwards to drive the injector tube of the glue dripping injector to move downwards relative to the injector inner core, and compressing a cavity formed by the injector tube and the injector inner core to extrude the sealing glue in the cavity onto the glass slide;

(2) drawing out the tray on which the mounting glue is dripped, and repeating the step (1) to drip the glass slide in the next tray;

(3) repeating the step (2), when the sealing rubber in the rubber dripping injector is used up, the upper annular groove of the injector tube clamps the inner core of the injector, the injector tube cannot move downwards continuously, and the control disc cannot rotate in the same direction any more; rotating the control disc in the opposite direction to drive the control rod and the nut to rotate upwards to drive the upper fixing plate to move upwards and drive the injector tube to move upwards relative to the inner core of the injector so as to restore the injector tube to the original position, opening the upper cover of the first shell and the cover body on the injector tube, and adding the sealing piece glue into the glue dripping injector;

(4) and (3) continuing repeating the steps (1) and (2) and carrying out glue dripping on the slide glass in the next tray.

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