CN112517098A

CN112517098A - Time-delay pressure-compensating pipettor for biomedical laboratory

Info

Publication number: CN112517098A
Application number: CN202011387609.0A
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Chongqing Kaizhou Hualan Biological Apheresis Plasma Co ltd
Priority date: 2020-12-01
Filing date: 2020-12-01
Publication date: 2021-03-19
Anticipated expiration: 2040-12-01
Also published as: CN112517098B

Abstract

The invention relates to the field of biomedical experimental instruments and discloses a time-delay pressure compensation pipettor for a biomedical laboratory, which comprises a pipettor tube body, wherein the bottom of the pipettor tube body is communicated with a liquid-transferring needle tube, the bottom of an inner cavity of the pipettor tube body is provided with a positioning pressure compensation tube, one side of the positioning pressure compensation tube is provided with a through groove communicated with the liquid-transferring needle tube, and the other side of the positioning pressure compensation tube is provided with an inner groove. This time delay pressure compensation pipettor is used in biomedical laboratory, through the structural design of negative pressure straw below location pressure compensation pipe, the cooperation pushes away the axle and takes out the autogyration coupling mechanism between the pressure piston, use the pipettor to carry out the ration and shift the solution in-process, utilize the upper magnetic plate to act on next magnetic plate earlier, make next magnetic plate move down, until the upper magnetic plate is rotatory behind the opposite side, the rebound resets under the spring action of next magnetic plate, thereby be convenient for extract solution before, it is more thorough to exhaust, the accuracy nature of solution transfer volume has been improved.

Description

Time-delay pressure-compensating pipettor for biomedical laboratory

Technical Field

The invention relates to the field of biomedical experimental instruments, in particular to a time-delay pressure-compensating pipettor for a biomedical laboratory.

Background

The biomedical technology is a bioengineering means which comprises the research and utilization of genes, viruses, gene methods, virus methods, cell culture, vaccine production, xenotransplantation, engineering, drug delivery, biological infection and the like, the medical biotechnology can greatly improve the medical level of human beings, the research of the biomedicine can not leave the biomedical experiment, wherein, a quantitative liquid transfer device is a common instrument in a biological laboratory and is used for transferring a microbial solution to carry out the experiment, and the accuracy of the transfer amount determines the accuracy of the experimental result.

However, current biomedical laboratory pipettor is in the use, when using the pipettor discharge solution, because of the consistency of part microorganism solution, part solution remains in the pipette needle department of pipettor, can't get rid of thoroughly, make the solution loss great when shifting, influence the accuracy nature of experimental result, and need the experimenter to continuously keep pressing the state of pipettor, with the drippage of waiting for microorganism solution, lead to efficiency reduction, and when extracting the solution early, easily because of the exhaust is not thorough, lead to extracting the solution volume and be little than the setting value, the accuracy nature of follow-up experimental result has been influenced equally.

Disclosure of Invention

The invention provides a time-delay pressure-compensating pipettor for a biomedical laboratory, which has the advantage of accurate pipetting quantity and solves the problems in the background technology.

In order to achieve the above purpose, the invention provides the following technical scheme to realize: a time-delay pressure-supplementing liquid transfer device for a biomedical laboratory comprises a liquid transfer device tube body, wherein a liquid transfer needle tube is communicated with the bottom of the liquid transfer device tube body, a positioning pressure-supplementing tube is arranged at the bottom of an inner cavity of the liquid transfer device tube body, a through groove communicated with the liquid transfer needle tube is formed in one side of the positioning pressure-supplementing tube, an inner groove is formed in the other side of the positioning pressure-supplementing tube and communicated with the through groove, a pressure-supplementing piston is fixedly connected to the bottom of the inner groove, a linkage shaft is fixedly connected to the middle of the top end of the pressure-supplementing piston, a lower magnetic plate is fixedly connected to the top end of the linkage shaft, the linkage shaft and the lower magnetic plate are movably sleeved with the top of the positioning pressure-supplementing tube, a supporting spring is fixedly connected to the bottom of the pressure-supplementing piston, a negative pressure suction tube positioned right above the positioning pressure-supplementing tube is arranged inside, the utility model discloses a suction and compression device, including negative pressure suction pipe, suction piston, last magnetic plate, push shaft, pressure piston, last magnetic plate, push shaft, pressure piston, last magnetic plate, the bottom of last magnetic plate is the same with the top magnetic pole of next magnetic plate, the top movable sleeve of pipettor body is equipped with the push shaft that only reciprocates, and pushes away the top of shaft and be equipped with the clamp plate that is located pipettor body top, push shaft's bottom extend to in the negative pressure suction pipe and with the transmission is connected with from rotating coupling mechanism between the suction piston, push shaft every time moves to behind the suction piston contact location pressure compensating pipe, and the rotatory half a circle of suction piston, be equipped with the outside extension spring that resets of movable sleeve at the push shaft between the top of.

Optionally, the diameter value of the universal driving shaft is smaller than that of the pressure supplementing piston, the outer side of the pressure supplementing piston is attached to the inner wall of the inner groove of the positioning pressure supplementing pipe, and the outer side of the universal driving shaft is attached to the inner wall of the top of the positioning pressure supplementing pipe.

Optionally, the quantifying device comprises a positioning screw rod movably sleeved in the pipettor tube body, the positioning screw rod is located on one side of the push shaft, the top end of the positioning screw rod extends out of the top end of the pipettor tube body and is fixedly connected with an adjusting rotating wheel, the quantifying device is provided with a driven plate located in the pipettor tube body in a threaded sleeve mode, the push shaft is fixedly connected with a positioning plate, the positioning plate is located below the driven plate, the top of the positioning plate is movably connected with the bottom of the driven plate, and a transparent plate located at the position of the driven plate is arranged on one side of the pipettor tube body.

Optionally, autogyration coupling mechanism include with the next linkage pipe of withholding piston top fixed connection and with push away epaxial linkage pipe of a shaft bottom fixed connection, and the bottom activity suit of going up linkage pipe is in the inside on next linkage pipe top, ball screw's top extends to the inside and the fixedly connected with limit baffle of last linkage pipe, the fixed cover in bottom of going up linkage pipe inner chamber is equipped with unidirectional rotation bearing, and unidirectional rotation bearing's middle part fixed cover is equipped with the ball swivel nut, the ball swivel nut cup joints with the ball screw thread.

The invention provides a time-delay pressure-compensating pipettor for a biomedical laboratory, which has the following beneficial effects:

1. this time delay pressure compensation pipettor is used in biomedical laboratory, through the structural design of negative pressure straw below location pressure compensation pipe, the cooperation pushes away the axle and takes out the autogyration coupling mechanism between the pressure piston, use the pipettor to carry out the ration and shift solution in-process, in the exhaust stage of extraction solution, utilize last magnetic sheet to act on next magnetic sheet earlier, make next magnetic sheet move down, until last magnetic sheet rotatory to the opposite side after, the rebound resets under the spring action of next magnetic sheet, thereby be convenient for extract solution before, it is more thorough to exhaust, in order to ensure that the extraction solution volume is more accurate, the accuracy nature of solution transfer volume has been improved.

2. According to the time-delay pressure compensation pipettor for the biomedical laboratory, in the easy stage of transferring and discharging the interior of the pipettor, the upper magnetic plate just rotates to the position above the lower magnetic plate, and the magnetic repulsion force of the upper magnetic plate on the lower magnetic plate is utilized to enable the lower magnetic plate to drive the linkage shaft and the pressure compensation piston to integrally move downwards, so that the pressure compensation piston moves downwards to compress and position the space of the inner groove of the pressure compensation pipe and the space between the liquid-transfering needle tubes, and the residual solution is continuously pressed out, so that the solution is transferred more thoroughly, the accurate effect of solution transfer is improved, and the accuracy of subsequent test results is facilitated; and do not need operating personnel to keep pressing in order to wait for the residual solution to drip, compare in present laboratory with ration pipettor, improved the transfer efficiency of solution.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the suction tube of FIG. 1 according to the present invention;

FIG. 3 is a schematic view of the self-rotating coupling mechanism of the structure of FIG. 2 according to the present invention;

fig. 4 is a bottom view of the pumping piston of the structure of fig. 2 according to the present invention.

In the figure: 1. a pipette body; 2. a pipette needle tube; 3. positioning a pressure compensating pipe; 301. a through groove; 302. a pressure compensating piston; 303. a linkage shaft; 304. a lower magnetic plate; 305. a support spring; 4. a negative pressure suction pipe; 401. pumping the piston; 402. an upper magnetic plate; 403. a return tension spring; 5. pushing the shaft; 501. positioning a plate; 6. a dosing device; 601. positioning a screw rod; 602. a passive plate; 603. adjusting the rotating wheel; 7. a self-rotating connection mechanism; 701. a lower linkage pipe; 702. an upper linkage pipe; 703. a ball screw; 704. a limit baffle; 705. a unidirectional rotation bearing; 706. ball thread sleeves.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, a time-delay pressure compensation pipette for biomedical laboratories comprises a pipette body 1, a pipette needle tube 2 is connected to the bottom of the pipette body 1, a positioning pressure compensation tube 3 is arranged at the bottom of an inner cavity of the pipette body 1, a through groove 301 communicated with the pipette needle tube 2 is formed in one side of the positioning pressure compensation tube 3, an inner groove is formed in the other side of the positioning pressure compensation tube 3, the inner groove is communicated with the through groove 301, a pressure compensation piston 302 is fixedly connected to the bottom of the inner groove, a linkage shaft 303 is fixedly connected to the middle of the top end of the pressure compensation piston 302, a lower magnetic plate 304 is fixedly connected to the top end of the linkage shaft 303, the linkage shaft 303 and the lower magnetic plate 304 are movably sleeved with the top of the positioning pressure compensation tube 3, a supporting spring 305 is fixedly connected to the bottom of the pressure compensation piston 302, the bottom end of the supporting spring 305 is fixedly connected to the bottom of the inner groove, a negative, the inner cavity of the negative pressure suction pipe 4 is communicated with the through groove 301, a suction piston 401 is movably sleeved in the inner cavity of the negative pressure suction pipe 4, an upper magnetic plate 402 corresponding to a lower magnetic plate 304 is arranged at the bottom of the suction piston 401, the bottom of the upper magnetic plate 402 has the same magnetic pole as the top of the lower magnetic plate 304, a push shaft 5 which only moves up and down is movably sleeved at the top of the pipette body 1, a pressing plate positioned above the pipette body 1 is arranged at the top of the push shaft 5, the bottom end of the push shaft 5 extends into the negative pressure suction pipe 4 and is in transmission connection with a self-rotating connecting mechanism 7 between the suction piston 401 and the push shaft 5, after the push shaft 5 moves down to the suction piston 401 to contact with the positioning pressure compensating pipe 3, the suction piston 401 rotates half a circle, a reset tension spring 403 which is movably sleeved outside the push shaft 5 is arranged between the suction piston 401 and the top of the inner cavity of the negative pressure suction pipe 4, in the using process, firstly, the amount of the solution to be extracted is adjusted through the self-rotating connecting mechanism 7, when the solution to be transferred is sucked by using a pipettor, the pipette needle tube 2 at the bottom of the pipettor is inserted into the solution to be extracted, after the pipettor body 1 is held, the pressing plate at the top of the pushing shaft 5 is pressed through fingers, so that the pushing shaft 5 drives the pumping piston 401 to move downwards until the pumping piston 401 contacts the top of the positioning pressure compensating tube 3, the pushing shaft 5 is continuously pressed to the bottom, the pumping piston 401 rotates for a half circle by using the self-rotating connecting mechanism 7 between the pushing shaft 5 and the pumping piston 401, the upper magnetic plate 402 just rotates to one side of the lower magnetic plate 304, then the pushing shaft 5 is loosened, under the pulling force of the reset tension spring 403, the pumping piston 401 moves upwards to the position regulated by the quantifying device 6, the solution is extracted into the pipettor, and then the pipette needle tube 2 at the bottom of the pipettor is placed into a container to be, the pushing shaft 5 drives the pumping piston 401 to move downwards by pressing the pressing plate at the top of the pushing shaft 5 until the pumping piston 401 contacts the top of the positioning pressure compensation pipe 3, the solution in the pipettor is extruded into the container, the pushing shaft 5 is continuously pressed to the bottom, the pumping piston 401 rotates half a circle by using the self-rotating connecting mechanism 7 between the pushing shaft 5 and the pumping piston 401, at the moment, the upper magnetic plate 402 just rotates to the upper part of the lower magnetic plate 304, the lower magnetic plate 304 drives the linkage shaft 303 and the pressure compensation piston 302 to integrally move downwards by using the magnetic repulsion force of the upper magnetic plate 402 to the lower magnetic plate 304, the space of the inner groove of the positioning pressure compensation pipe 3 and the space between the needle tube liquid-transfering 2 are compressed downwards by using the pressure compensation piston 302, the residual solution is continuously pressed out, the solution transfer is more thorough, the precise effect of the solution transfer is improved, and the precision of the subsequent test result is facilitated, and the residual solution does not need to drop after being pressed, the transfer efficiency of the solution is improved, in the whole process of extracting the solution, in the exhaust stage, the pumping piston 401 firstly contacts the positioning pressure compensating pipe 3 and then rotates for a half circle, namely, in the exhaust stage, the upper magnetic plate 402 firstly acts on the lower magnetic plate 304, so that the lower magnetic plate 304 moves downwards until the upper magnetic plate 402 rotates to the other side, and the lower magnetic plate 304 moves upwards under the elastic action of the supporting spring 305 to reset, so that the solution can be extracted conveniently, the exhaust is more thorough, the solution extraction amount is more accurate, and the accuracy of the solution transfer amount is further improved.

Wherein, the diameter value of universal driving shaft 303 is less than the diameter value of pressure supplementing piston 302, and the outside of pressure supplementing piston 302 and the laminating of the inner wall of location pressure supplementing pipe 3 inside groove department, the outside of universal driving shaft 303 and the laminating of the top inner wall of location pressure supplementing pipe 3, ensure that pressure supplementing piston 302 and universal driving shaft 303 can be stable reciprocate, thereby be convenient for utilize the magnetic repulsion of upper magnetic plate 402 to lower magnetic plate 304, when shifting solution, provide the time delay pressure supplementing, thereby be favorable to the thorough discharge of solution, reduce the loss of solution transfer in-process, in order to improve the accuracy of solution transfer volume, thereby ensure the accuracy nature of test result.

Wherein, the proportioning device 6 is including the movable suit at the inside location lead screw 601 of pipettor body 1, and location lead screw 601 is located one side of push shaft 5, the top of location lead screw 601 extends the outside and the fixedly connected with adjustment wheel 603 on pipettor body 1 top, threaded cover is equipped with the passive board 602 that is located pipettor body 1 on the proportioning device 6, fixedly connected with locating plate 501 on the push shaft 5, locating plate 501 is located the below of passive board 602, and the top of locating plate 501 and the bottom swing joint of passive board 602, one side of pipettor body 1 is equipped with the transparent plate that is located passive board 602 department, it is rotatory to drive location lead screw 601 through rotating adjustment wheel 603, adjust the height of passive board 602, thereby adjust the difference in height that pump piston 401 reciprocated, and then confirm the volume of solution extraction, so that the experimenter operates.

Wherein, the self-rotation connecting mechanism 7 comprises a lower linkage pipe 701 fixedly connected with the top of the pumping piston 401 and an upper linkage pipe 702 fixedly connected with the bottom end of the push shaft 5, the bottom end of the upper linkage pipe 702 is movably sleeved inside the top end of the lower linkage pipe 701, the top end of the ball screw 703 extends to the inside of the upper linkage pipe 702 and is fixedly connected with a limit baffle 704, the bottom of the inner cavity of the upper linkage pipe 702 is fixedly sleeved with a unidirectional rotation bearing 705, the middle part of the unidirectional rotation bearing is fixedly sleeved with a ball screw sleeve 706, the ball screw sleeve 706 is in threaded sleeve connection with the ball screw 703, in the process of downward movement of the push shaft 5, after the pumping piston 401 contacts with the positioning pressure compensating pipe 3, the push shaft 5 is continuously pressed to move downward, so that the push shaft 5 drives the upper linkage pipe 702 at the bottom to move downward relative to the lower linkage pipe 701, at this time, the unidirectional rotation bearing 705 is in a locked state, under the action of the ball, the ball screw sleeve 706, the unidirectional rotating bearing 705, the lower linkage pipe 701 and the pumping piston 401 are enabled to integrally rotate until the bottom end of the upper linkage pipe 702 contacts the bottom of the groove inner cavity of the lower linkage pipe 701, the pumping piston 401 rotates for a half circle, so that the upper magnetic plate 402 is controlled to rotate to the other side of the lower magnetic plate 304 when solution is pumped, and when the solution is extruded, the upper magnetic plate 402 is controlled to rotate to the upper side of the lower magnetic plate 304, so that the solution is transferred more thoroughly, and the accuracy of the solution transfer amount is guaranteed.

When the device is used, firstly, the adjusting rotating wheel 603 is rotated to adjust the position of the driven plate 602, the amount of solution transfer is controlled, the pipette needle tube 2 at the bottom of the pipette is inserted into the solution to be extracted, after the pipette body 1 is held, the pressing plate at the top of the pushing shaft 5 is pressed by fingers, so that the pushing shaft 5 drives the pumping piston 401 to move downwards until the pumping piston 401 contacts the top of the positioning pressure compensating tube 3, the pushing shaft 5 is continuously pressed to the bottom, the pumping piston 401 rotates for a half circle by using the self-rotating connecting mechanism 7 between the pushing shaft 5 and the pumping piston 401, the upper magnetic plate 402 just rotates to one side of the lower magnetic plate 304, then the pushing shaft 5 is loosened, the pumping piston 401 moves upwards to the position regulated by the quantifying device 6 under the action of the pulling force of the reset tension spring 403, the solution is extracted into the pipette by using negative pressure, and the pipette needle tube 2 at the bottom is placed into a container to be transferred, the pushing shaft 5 drives the pumping piston 401 to move downwards by pressing the pressing plate on the top of the pushing shaft 5 until the pumping piston 401 contacts the top of the positioning pressure compensation pipe 3, the solution in the pipettor is extruded into the container, the pushing shaft 5 is continuously pressed to the bottom, the self-rotation connecting mechanism 7 between the pushing shaft 5 and the pumping piston 401 is utilized to enable the pumping piston 401 to rotate for a half circle, at the moment, the upper magnetic plate 402 just rotates to the upper part of the lower magnetic plate 304, the magnetic repulsion force of the upper magnetic plate 402 to the lower magnetic plate 304 is utilized to enable the lower magnetic plate 304 to drive the linkage shaft 303 and the pressure compensation piston 302 to integrally move downwards, and the pressure compensation piston 302 is utilized to move downwards to compress the space of the inner groove of the positioning pressure compensation pipe 3 and the space between the needle pipe liquid transfer pipes 2, so as to continuously press out the residual solution.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a biomedical laboratory is with time delay pressure compensation pipettor, includes pipettor body (1), and the bottom of pipettor body (1) is switched on and is had pipette needle tubing (2), its characterized in that: the bottom of the inner cavity of the pipette body (1) is provided with a positioning pressure compensating pipe (3), one side of the positioning pressure compensating pipe (3) is provided with a through groove (301) communicated with the pipette needle tube (2), the other side of the positioning pressure compensating pipe (3) is provided with an inner groove communicated with the through groove (301), the bottom of the inner groove is fixedly connected with a pressure compensating piston (302), the middle part of the top end of the pressure compensating piston (302) is fixedly connected with a linkage shaft (303), the top end of the linkage shaft (303) is fixedly connected with a lower magnetic plate (304), the linkage shaft (303) and the lower magnetic plate (304) are both movably sleeved with the top of the positioning pressure compensating pipe (3), the bottom of the pressure compensating piston (302) is fixedly connected with a supporting spring (305), the bottom end of the supporting spring (305) is fixedly connected with the bottom of the inner groove, a negative pressure suction pipe (4) positioned right above the positioning pressure compensating pipe (3) is arranged in the pipette, and the inner cavity of the negative pressure suction pipe (4) is communicated with the through groove (301), the inner cavity of the negative pressure suction pipe (4) is movably sleeved with a pressure suction piston (401), the bottom of the pressure suction piston (401) is provided with an upper magnetic plate (402) corresponding to a lower magnetic plate (304), the bottom of the upper magnetic plate (402) is the same as the top magnetic pole of the lower magnetic plate (304), the top of the pipette body (1) is movably sleeved with a push shaft (5) only moving up and down, the top of the push shaft (5) is provided with a pressing plate positioned above the pipette body (1), the bottom of the push shaft (5) extends into the negative pressure suction pipe (4) and is in transmission connection with a self-rotating connection mechanism (7) with the pressure suction piston (401), after the push shaft (5) moves down to the pressure suction piston (401) to contact with the positioning pressure compensating pipe (3), the pressure suction piston (401) rotates for a half a circle, and a movable sleeve is arranged between the top of the push shaft (5) and the top of the inner cavity of the suction pipe (4) The reset tension spring (403), the inside of pipettor body (1) is equipped with the proportioning device (6) that is located push shaft (5) one side.

2. The time-lapse pressure-compensation pipette for the biomedical laboratory according to claim 1, wherein: the diameter value of universal driving shaft (303) is less than the diameter value of pressure supplementing piston (302), and the outside of pressure supplementing piston (302) is laminated with the inner wall of location pressure supplementing pipe (3) inside groove, the outside of universal driving shaft (303) is laminated with the top inner wall of location pressure supplementing pipe (3).

3. The time-lapse pressure-compensation pipette for the biomedical laboratory according to claim 1, wherein: the utility model discloses a pipette, including the quantifying device (6) including the movable suit at the inside location lead screw (601) of pipettor body (1), and location lead screw (601) are located one side of push shaft (5), the top of location lead screw (601) extends pipettor body (1) top outside and fixedly connected with adjusting wheel (603), threaded cover is equipped with passive board (602) that are located pipettor body (1) on quantifying device (6), fixedly connected with locating plate (501) on push shaft (5), locating plate (501) are located the below of passive board (602), and the top of locating plate (501) and the bottom swing joint of passive board (602), one side of pipettor body (1) is equipped with the transparent plate that is located passive board (602) department.

4. The time-lapse pressure-compensation pipette for the biomedical laboratory according to claim 1, wherein: autogyration coupling mechanism (7) include with the lower linkage pipe (701) of the fixed connection of pump-out piston (401) top and with push away epaxial linkage pipe (702) of (5) bottom fixed connection, and the bottom activity suit of upper linkage pipe (702) is in the inside on lower linkage pipe (701) top, the top of ball screw (703) extends to the inside and the fixedly connected with limit baffle (704) of upper linkage pipe (702), the fixed suit in bottom of upper linkage pipe (702) inner chamber has unidirectional rotation bearing (705), and the fixed suit in middle part of unidirectional rotation bearing (705) has ball swivel nut (706), ball swivel nut (706) and ball screw (703) thread socket.