CN112515042A - Pet nutrition paste prepared from euphausia superba protein peptide and preparation method thereof - Google Patents
Pet nutrition paste prepared from euphausia superba protein peptide and preparation method thereof Download PDFInfo
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- CN112515042A CN112515042A CN202011551288.3A CN202011551288A CN112515042A CN 112515042 A CN112515042 A CN 112515042A CN 202011551288 A CN202011551288 A CN 202011551288A CN 112515042 A CN112515042 A CN 112515042A
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- oil
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- enzymolysis
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The invention relates to the technical field of pet feed, in particular to a pet nutrition paste prepared from euphausia superba protein peptide and a preparation method thereof, wherein the method comprises the following steps: s1 preparation of euphausia superba protein peptide: taking first-stage residue of antarctic krill as a raw material, and performing pretreatment, enzymolysis combined debittering, refining and concentration to obtain an antarctic krill protein peptide solution; s2 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water-based base material and an oil-based material, wherein the water-based base material comprises an antarctic krill protein peptide solution; s3 mixing the nutritional paste forming matrix: mixing the water system base material and the oil system material to form an oil-in-water system, and obtaining the nutritional paste with uniform shape. According to the invention, the first-stage residue of the euphausia superba left after the euphausia superba powder is subjected to lipid extraction is used as a raw material, and the characteristics of low lipid content and high protein content are utilized to prepare the nutritional paste product which is rich in euphausia superba protein peptides and has good nutrition, efficacy and palatability, so that the comprehensive utilization rate of the euphausia superba is improved.
Description
Technical Field
The invention relates to the technical field of pet feeds, and particularly relates to a pet nutrition paste prepared from euphausia superba protein peptide and a preparation method thereof.
Background
Pets, also known as "companion animals," play an increasingly important role in our lives as important human emotional setbacks, often viewed as one of the family members. Their nutrition and health have become a prominent challenge. Particularly, with the social progress, the concept of pet feeding is greatly changed, and the diet requirements of people for pets are higher and higher. Therefore, the pet food industry is in need of transformation and upgrading, particularly, the innovation of products is needed, and a pet food industry chain which is more nutritional, healthier and more targeted is developed according to different types and requirements of pets.
The biological reserves of the antarctic krill are huge, and the antarctic krill are listed as important strategic resources in various countries, and belong to one of three extremely important marine resources to be developed. The nutritional paste for the pet, which is prepared from the antarctic krill lipid by taking the antarctic krill powder as a raw material, is rich in flavor and comprehensive in nutritional function, and is rich in DHA and astaxanthin, but residues left after the lipid is extracted from the antarctic krill powder contain more than 50% (dry weight) of protein resources, the composition of amino acids is rich, the total amount of 8 amino acids necessary for a human body accounts for more than 40% of the total protein content, and the nutritional value is high.
Therefore, the pet nutrition paste rich in the euphausia superba protein peptide and the preparation method thereof, which are based on the preparation of the pet nutrition paste rich in the euphausia superba lipid, can be prepared by utilizing the residues generated in the processing process, so that the comprehensive utilization of the euphausia superba resources is improved, and the additional value of the product is increased.
Disclosure of Invention
Aiming at the problem of waste of protein resources caused by waste of residues generated in the processing process of the pet nutritional paste rich in antarctic krill lipid, the invention provides the pet nutritional paste prepared by utilizing the antarctic krill protein peptide and the preparation method thereof.
In a first aspect, the present invention provides a method for preparing a pet nutritional paste using euphausia superba protein peptides, the method comprising:
s1 preparation of euphausia superba protein peptide: taking first-stage residue of antarctic krill as a raw material, and performing pretreatment, enzymolysis combined debittering, refining and concentration to obtain an antarctic krill protein peptide solution;
s2 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water-based base material and an oil-based material, wherein the water-based base material comprises an antarctic krill protein peptide solution;
s3 mixing the nutritional paste forming matrix: mixing the water system base material and the oil system material to form an oil-in-water system, and obtaining the nutritional paste with uniform shape.
Further, the first-stage residue of the Antarctic krill refers to residue left after the Antarctic krill lipid-enriched pet nutrition paste is prepared, and specifically is solid residue left after the Antarctic krill powder is extracted by ethanol.
Further, the S1 preparation of the euphausia superba protein peptide comprises:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the raw material euphausia superba, uniformly stirring, and then adding acid protease for enzymolysis to obtain a treatment solution A;
(2) enzymolysis and combined debitterizing: carrying out enzymolysis on the treatment solution A by using flavourzyme and trypsin, adding sugar into the system after the enzymolysis is finished, stirring, dissolving and preserving heat to obtain a treatment solution B;
(3) refining: refining the treated liquid B by ultrafiltration, and collecting filtrate to obtain treated liquid C;
(4) concentration: and (4) carrying out reduced pressure concentration treatment on the treated liquid C to obtain the euphausia superba protein peptide solution.
Further, the preparation of the S1 euphausia superba protein peptide specifically comprises the following steps:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into a first-stage residue of raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 3-5, wherein the mass ratio of citric acid to water in the citric acid water solution is 0.015-0.1: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 500-1000 u/g, stirring for 2-4 h at 35-45 ℃ under enzymolysis conditions, and obtaining a treatment solution A after enzymolysis;
(2) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A, adjusting the pH value to 7.0-8.0, then adding flavourzyme and trypsin for enzymolysis of the treatment liquid A, wherein the concentration of the flavourzyme is 100-200 u/g, the concentration of the trypsin is 50-100 u/g, stirring for 2-4 h at 35-45 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 1-10: 100, stirring and dissolving the sugar including at least one of glucose, fructose and xylose, heating the system to 90-100 ℃, preserving heat for 1-4 hours, and cooling to room temperature after heat preservation to obtain a treatment solution B;
(3) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;
(4) concentration: and (3) carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of-0.06-0.09 MPa and temperature of 60-90 ℃ to enable the solid content in the liquid system to be not lower than 20%, so as to obtain the euphausia superba protein peptide solution.
Further, the S2 nutritional paste forming matrix construction comprises:
(1) preparing a water system base material: mixing Euphausia superba protein peptide solution, excipient, milk powder, glucose, maltose, white granulated sugar and vitamins, heating to dissolve after completely hydrating, and stirring uniformly to prepare a water system base material;
(2) preparing oil materials: mixing animal fat, emulsifier, auxiliary emulsifier, and fat-soluble vitamins, stirring to disperse uniformly, and making into oil material.
Further, the S2 nutritional paste forming matrix is specifically constructed by the following steps:
(1) preparing a water system base material: weighing 110 parts of euphausia superba protein peptide solution, 3-10 parts of excipients, 20-30 parts of milk powder, 10-20 parts of glucose, 5-10 parts of maltose, 10-20 parts of white granulated sugar and 0.5-2 parts of vitamins according to parts by weight, mixing, heating and dissolving at 40-90 ℃ after complete hydration, and uniformly stirring to prepare a water system base material;
the excipient comprises at least one of pectin, sodium alginate, gelatin, agar and xanthan gum;
(2) preparing oil materials: weighing 100 parts of animal fat, 1-10 parts of emulsifier, 0.1-5 parts of co-emulsifier and 0.1-2 parts of fat-soluble vitamin according to parts by weight, mixing, and stirring until the mixture is uniformly dispersed to prepare an oil material;
the animal oil comprises at least one of fish oil, shrimp oil, pork, chicken, beef tallow and mutton fat;
the emulsifier comprises at least one of sucrose ester, polyglycerol stearate, monoglyceride, diglycerol fatty acid ester, tween and lecithin.
Further, the S3 nutritional paste forming matrix is specifically mixed as follows:
respectively heating a water system base material and an oil system material to 70-100 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1-0.5, homogenizing and emulsifying while adding until the system is uniform, forming a stable oil-in-water system, and obtaining the nutritional paste with uniform shape without free oil materials.
Further, the homogeneous emulsification is shearing homogeneous emulsification, the rotating speed of the shearing homogeneous emulsification is 1000-30000 rpm, and the time is 5-30 min.
Further, the method also comprises the steps of S4 sterilization and packaging: and (3) preserving the heat of the nutrient paste at 65-70 ℃ for sterilization for 15min, and then subpackaging according to the production requirement.
In a second aspect, the invention provides a pet nutrition paste prepared by the above method.
Furthermore, the mass fraction of the euphausia superba protein peptide in the nutritional paste is not less than 10%.
The beneficial effect of the invention is that,
the method takes the comprehensive utilization of antarctic krill resources as a starting point, prepares the antarctic krill protein peptide by utilizing residues generated in the processing process through the technologies of pretreatment, enzymolysis combined debitterizing, graded fine filtration (intercepting enzymolysis liquid according to molecules) and the like, and then prepares a nutrient paste product which is rich in the antarctic krill euphausia superba protein peptide and has good nutrition, efficacy and palatability through the formula matrix effect and the shaping design, promotes the development of high-end pet nutriments, improves the comprehensive utilization rate of the antarctic krill and improves the added value of the product.
The preparation method of the pet nutrition paste provided by the invention is easy to operate, and by designing a formula and optimizing a process, a final product has good stability, no bitter taste and no phenomenon of oil-water separation after long-time storage; the method has simple and mild process, ensures that the whole process is environment-friendly and has low energy consumption, is beneficial to industrial popularization, and has positive significance for promoting the development of high-end pet nutrition functional product industry.
The pet nutrition paste provided by the invention takes the residual first-grade residue of the Antarctic krill after the Antarctic krill lipid-rich pet nutrition paste is prepared as a raw material, the lipid content in the raw material is low through analysis, the protein content reaches more than 50% (dry weight) and the amino acid composition is rich, wherein the total amount of 8 amino acids necessary for a human body accounts for more than 40% of the total protein content, the growth and metabolism can be effectively promoted, the immunity of the organism is improved, the effects of preventing diseases and aging are achieved, and the nutrition value is high.
According to the nutritional paste for pets, the accelerated test predicts that after the nutritional paste is stored for 12 months at 25 ℃, the paste is kept uniform, and no oil-water separation phenomenon can be seen by naked eyes, so that the nutritional paste for euphausia superba protein peptide prepared by the method has good stability; the sensory indexes of the nutritional paste are as follows:
color: the color is yellow brown and uniform;
taste and smell: the flavor is strong, and the product can be melted in the mouth;
the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;
impurities: no visible impurities.
Drawings
In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
Fig. 1 is a flow chart of a method for preparing a pet nutritional paste by using euphausia superba protein peptide in the embodiment of the invention.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiment of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A pet nutrition paste prepared from euphausia superba protein peptide is prepared by the following steps:
s1 preparation of euphausia superba protein peptide:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 3, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.015: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 500u/g, the enzymolysis condition is stirring for 2 hours at 35 ℃, and obtaining a treatment solution A after the enzymolysis is finished;
the first-grade residue of the antarctic krill refers to residue left after the antarctic krill lipid-rich pet nutrition paste is prepared, and the specific preparation method of the first-grade residue of the antarctic krill comprises the following steps:
adding an ethanol water solution with the concentration of 0.85g/L into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 2: 1, extracting for 1 time for 0.5h, and collecting the extracted solid residue, namely the first-stage residue of the euphausia superba;
(2) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 7.0, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 100u/g, the concentration of the trypsin is 50u/g, the enzymolysis condition is stirring for 2 hours at 35 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 1: 100, stirring and dissolving sugar including glucose, heating the system to 90 ℃, preserving heat for 1h, and cooling to room temperature after heat preservation to obtain a treatment solution B;
(3) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;
(4) concentration: carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of-0.06 MPa and 60 ℃ to ensure that the content of solid matters in a liquid system is not lower than 20 percent, thus obtaining a protein peptide solution of the antarctic krill;
s2 construction of a forming matrix of the nutritional paste:
(1) preparing a water system base material: weighing 110 parts of euphausia superba protein peptide solution, 3 parts of excipients, 20 parts of milk powder, 10 parts of glucose, 5 parts of maltose, 10 parts of white granulated sugar and 0.5 part of vitamin according to parts by weight, mixing, heating and dissolving at 40 ℃ after complete hydration, and uniformly stirring to prepare a water system base material, wherein the excipients comprise pectin;
(2) preparing oil materials: weighing 100 parts of animal fat, 1 part of emulsifier, 0.1 part of co-emulsifier and 0.1 part of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing an oil material, wherein the animal fat comprises beef tallow, and the emulsifier comprises the components in a volume ratio of 1: 1 sucrose ester, polyglyceryl stearate;
s3 mixing the nutritional paste forming matrix:
respectively heating the water system base material and the oil system material to 70 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1, adding while homogenizing and emulsifying until the system is uniform, forming a stable oil-in-water system without free oil materials, and obtaining the uniformly-shaped nutritional paste;
wherein the homogenizing emulsification is shearing homogenizing emulsification, the rotating speed of the shearing homogenizing emulsification is 1000rpm, and the time is 5 min;
s4, sterilization and packaging: sterilizing the nutritional paste at 65 deg.C for 15min, and packaging according to production requirement.
Example 1 the eating method of the pet nutrition paste is to eat the paste instantly after uncovering, and about 1-5 g of the paste is taken every day according to the nutrition requirement.
Example 2
A pet nutrition paste prepared from euphausia superba protein peptide is prepared by the following steps:
s1 preparation of euphausia superba protein peptide:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 5, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.1: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 1000u/g, the enzymolysis condition is stirring for 4 hours at 45 ℃, and obtaining a treatment solution A after the enzymolysis is finished;
the first-grade residue of the antarctic krill refers to residue left after the antarctic krill lipid-rich pet nutrition paste is prepared, and the specific preparation method of the first-grade residue of the antarctic krill comprises the following steps:
adding 1g/L ethanol water solution into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 10: 1, extracting for 3 times for 4 hours, and collecting the extracted solid residue to obtain first-stage residue of the euphausia superba;
(2) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 8.0, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 200u/g, the concentration of the trypsin is 100u/g, the enzymolysis condition is stirring for 4 hours at 45 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 10: 100, stirring and dissolving sugar including xylose, heating the system to 100 ℃, preserving heat for 4 hours, and cooling to room temperature after heat preservation to obtain a treatment solution B;
(3) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;
(4) concentration: carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of 0.09MPa and temperature of 90 ℃ to ensure that the content of solid matters in a liquid system is not lower than 20 percent, thus obtaining a protein peptide solution of the antarctic krill;
s2 construction of a forming matrix of the nutritional paste:
(1) preparing a water system base material: weighing 110 parts of euphausia superba protein peptide solution, 10 parts of an excipient, 30 parts of milk powder, 20 parts of glucose, 10 parts of maltose, 20 parts of white granulated sugar and 2 parts of vitamin according to parts by weight, mixing, heating and dissolving at 90 ℃ after complete hydration, and uniformly stirring to prepare a water-system base material, wherein the excipient comprises the following components in parts by weight: 1 sodium alginate and lac;
(2) preparing oil materials: weighing 100 parts of animal fat, 10 parts of emulsifier, 5 parts of co-emulsifier and 2 parts of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing an oil material, wherein the animal fat comprises mutton fat, and the emulsifier comprises monoglyceride;
s3 mixing the nutritional paste forming matrix:
respectively heating the water system base material and the oil system material to 100 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.5, adding while homogenizing and emulsifying until the system is uniform, forming a stable oil-in-water system without free oil materials, and obtaining the uniformly-shaped nutritional paste;
wherein the homogenizing emulsification is shearing homogenizing emulsification, the rotating speed of the shearing homogenizing emulsification is 30000rpm, and the time is 30 min;
s4, sterilization and packaging: sterilizing the nutritional paste at 70 deg.C for 15min, and packaging according to production requirement.
Example 2 the eating method of the pet nutrition paste is to eat the paste instantly after uncovering, and about 1-5 g of the paste is taken every day according to the nutrition requirement.
Example 3
A pet nutrition paste prepared from euphausia superba protein peptide is prepared by the following steps:
s1 preparation of euphausia superba protein peptide:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 4, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.05: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 800u/g, the enzymolysis condition is stirring for 3 hours at 40 ℃, and obtaining a treatment solution A after the enzymolysis is finished;
the first-grade residue of the antarctic krill refers to residue left after the antarctic krill lipid-rich pet nutrition paste is prepared, and the specific preparation method of the first-grade residue of the antarctic krill comprises the following steps:
adding 1g/L ethanol water solution into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 6: 1, extracting for 2 times for 2 hours, and collecting the extracted solid residue to obtain first-stage residue of the euphausia superba;
(2) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 7.5, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 150u/g, the concentration of the trypsin is 75u/g, the enzymolysis condition is stirring for 3 hours at 40 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 6: 100, stirring and dissolving sugar comprising fructose, heating the system to 100 ℃, preserving heat for 3 hours, and cooling to room temperature after heat preservation to obtain a treatment solution B;
(3) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;
(4) concentration: carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of-0.03 MPa and 80 ℃ to ensure that the content of solid matters in a liquid system is not lower than 20 percent, thus obtaining a protein peptide solution of the antarctic krill;
s2 construction of a forming matrix of the nutritional paste:
(1) preparing a water system base material: weighing 110 parts of euphausia superba protein peptide solution, 8 parts of an excipient, 25 parts of milk powder, 15 parts of glucose, 7 parts of maltose, 13 parts of white granulated sugar and 1 part of vitamin according to parts by weight, mixing, heating and dissolving at 60 ℃ after complete hydration, and uniformly stirring to prepare a water-system base material, wherein the excipient comprises the following components in parts by weight: 1 gelatin, agar;
(2) preparing oil materials: weighing 100 parts of animal fat, 7 parts of emulsifier, 3 parts of co-emulsifier and 0.8 part of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing the oil material, wherein the animal fat comprises the components in volume ratio of 1: 2, the fish oil and the shrimp oil and the emulsifier comprise tween;
s3 mixing the nutritional paste forming matrix:
respectively heating the water system base material and the oil system material to 80 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.3, adding while homogenizing and emulsifying until the system is uniform, forming a stable oil-in-water system without free oil materials, and obtaining the nutritional paste with uniform shaping, wherein the homogenizing and emulsifying is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 15000rpm, and the time is 15 min;
s4, sterilization and packaging: sterilizing the nutritional paste at 70 deg.C for 15min, and packaging according to production requirement.
Example 3 the eating method of the pet nutrition paste is to eat the paste instantly after uncovering, and about 1-5 g of the paste is taken every day according to the nutrition requirement.
Comparative example 1
Comparative example 1 differs from example 3 in that S1 antarctic krill protein peptide preparation differs, and the preparation of the antarctic krill protein peptide in comparative example 1 lacks a step of raw material pretreatment.
Comparative example 2
Comparative example 2 differs from example 3 in that S1 antarctic krill protein peptide was prepared differently and S1 in comparative example 2 lacks the enzymatic hydrolysis combined with debittering step.
Comparative example 3
The difference between the comparative example 3 and the example 3 is that the S2 nutrition paste forming matrix is constructed and the S3 nutrition paste forming matrix is mixed differently, and the S2 nutrition paste forming matrix and the S3 nutrition paste forming matrix of the comparative example 3 are mixed as follows:
s2 construction of a forming matrix of the nutritional paste:
(1) preparing a water system base material: weighing 8 parts of excipient, 25 parts of milk powder, 15 parts of glucose, 7 parts of maltose, 13 parts of white granulated sugar and 1 part of vitamin according to the parts by weight, and uniformly mixing to prepare a water-based base material, wherein the excipient comprises the following components in a mass ratio of 2: 1 gelatin, agar;
(2) preparing oil materials: weighing 100 parts of animal fat, 7 parts of emulsifier, 3 parts of co-emulsifier and 0.8 part of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing the oil material, wherein the animal fat comprises the components in a volume ratio of 1: 2, the fish oil and the shrimp oil and the emulsifier comprise tween;
s3 mixing the nutritional paste forming matrix:
respectively heating the water system base material and the oil system material to 80 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: and 0.3, simultaneously adding 110 parts of euphausia superba protein peptide solution into the system, and homogenizing and emulsifying while adding until the system is uniform to form a stable oil-in-water system without free oil materials to obtain the uniformly-shaped nutrient paste, wherein the homogenizing and emulsifying is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 15000rpm, and the time is 15 min.
Test example 1 palatability evaluation
Palatability comparison tests were performed using the international universal "double pot" method to evaluate the differences in palatability of the nutritional pastes prepared in example 3, comparative example 1 and comparative example 2. 18 adult cats are selected as test animals and are averagely divided into two groups, the weight and the male and female of each group are kept balanced as much as possible, the test period is 1 day, the adult cats are fed with one meal respectively in the morning and evening, the feeding duration of each meal is 20 minutes, and sufficient drinking water supply is ensured in the test period. The food intake was calculated by weighing 40g per group after the end of the test feeding, and the food intake rate was calculated from the food intake rate (%) -food intake/food intake × 100%, and the results are shown in table 1 below.
TABLE 1 feed intake rates for nutritional pastes of example 3 and comparative examples 1-2
From the data, the feed intake rate of the nutrient paste prepared in the embodiment 3 of the invention is obviously higher than that of the comparative example 1, which shows that the raw material pretreatment process adopted by the invention can improve the hydrolysis degree of protein, and the generated hydrolysate has positive effects on the flavor and palatability of the nutrient paste; the feed intake rate of example 3 is significantly higher than that of comparative example 2, which shows that the enzymolysis and debittering process can improve the flavor and palatability of the hydrolysate. In conclusion, the test results of the test example show that: the feed intake rate of the nutrient paste prepared in the embodiment 3 of the invention is more than 90%, which is obviously higher than that of the nutrient paste prepared in the comparative examples 1 and 2, and the invention adopts the pretreatment of raw materials and the enzymolysis combined debitterizing process, so that the palatability of the prepared nutrient paste can be obviously improved, and the popularization and application values are high.
Test example 2 stability evaluation
The nutritional pastes prepared in example 3 and comparative example 3 were subjected to paste stability evaluation using an accelerated storage test. Observing the state of the two groups of nutrient pastes after the two groups of nutrient pastes are stored in a stability test box at 40 ℃ for 10 days, and taking whether the oil-water separation phenomenon visible to the naked eye occurs in the pastes as an evaluation index. The evaluation results are shown in table 2 below.
Table 2 example 3 and comparative example 3 paste state
The above test results show that: accelerated tests predict that the nutrient paste prepared in example 3 is uniform after being stored at 40 ℃ for 10 days, and no oil-water separation phenomenon can be seen by naked eyes; the nutrient paste prepared in the comparative example 3 has obvious oil-water separation phenomenon. The pet nutrition paste prepared by the process of the embodiment has good stability.
Although the present invention has been described in detail by referring to the drawings in connection with the preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A method for preparing a pet nutrition paste by using euphausia superba protein peptide, which is characterized by comprising the following steps:
s1 preparation of euphausia superba protein peptide: taking first-stage residue of antarctic krill as a raw material, and performing pretreatment, enzymolysis combined debittering, refining and concentration to obtain an antarctic krill protein peptide solution;
s2 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water-based base material and an oil-based material, wherein the water-based base material comprises an antarctic krill protein peptide solution;
s3 mixing the nutritional paste forming matrix: mixing the water system base material and the oil system material to form an oil-in-water system, and obtaining the nutritional paste with uniform shape.
2. The method of claim 1, wherein the S1 Euphausia superba protein peptide preparation comprises:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the raw material euphausia superba, uniformly stirring, and then adding acid protease for enzymolysis to obtain a treatment solution A;
(2) enzymolysis and combined debitterizing: carrying out enzymolysis on the treatment solution A by using flavourzyme and trypsin, adding sugar into the system after the enzymolysis is finished, stirring, dissolving and preserving heat to obtain a treatment solution B;
(3) refining: refining the treated liquid B by ultrafiltration, and collecting filtrate to obtain treated liquid C;
(4) concentration: and (4) carrying out reduced pressure concentration treatment on the treated liquid C to obtain the euphausia superba protein peptide solution.
3. The method of claim 2, wherein the S1 Euphausia superba protein peptide is produced by:
(1) pretreatment of raw materials: adding a citric acid aqueous solution into a first-stage residue of raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 3-5, wherein the mass ratio of citric acid to water in the citric acid water solution is 0.015-0.1: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 500-1000 u/g, stirring for 2-4 h at 35-45 ℃ under enzymolysis conditions, and obtaining a treatment solution A after enzymolysis;
(2) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 7.0-8.0, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 100-200 u/g, the concentration of the trypsin is 50-100 u/g, the enzymolysis condition is stirring for 2-4 h at 35-45 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 1-10: 100, stirring and dissolving the sugar including at least one of glucose, fructose and xylose, heating the system to 90-100 ℃, preserving heat for 1-4 hours, and cooling to room temperature after heat preservation to obtain a treatment solution B;
(3) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;
(4) concentration: and (3) carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of-0.06-0.09 MPa and temperature of 60-90 ℃ to enable the solid content in the liquid system to be not lower than 20%, so as to obtain the euphausia superba protein peptide solution.
4. The method of claim 1, wherein the S2 nutritional paste forming matrix construction comprises:
(1) preparing a water system base material: mixing Euphausia superba protein peptide solution, excipient, milk powder, glucose, maltose, white granulated sugar and vitamins, heating to dissolve after completely hydrating, and stirring uniformly to prepare a water system base material;
(2) preparing oil materials: mixing animal fat, emulsifier, auxiliary emulsifier, and fat-soluble vitamins, stirring to disperse uniformly, and making into oil material.
5. The method of claim 4, wherein the S2 nutritional paste forming matrix is configured to:
(1) preparing a water system base material: weighing 110 parts of euphausia superba protein peptide solution, 3-10 parts of excipients, 20-30 parts of milk powder, 10-20 parts of glucose, 5-10 parts of maltose, 10-20 parts of white granulated sugar and 0.5-2 parts of vitamins according to parts by weight, mixing, heating and dissolving at 40-90 ℃ after complete hydration, and uniformly stirring to prepare a water system base material;
the excipient comprises at least one of pectin, sodium alginate, gelatin, agar and xanthan gum;
(2) preparing oil materials: weighing 100 parts of animal fat, 1-10 parts of emulsifier, 0.1-5 parts of co-emulsifier and 0.1-2 parts of fat-soluble vitamin according to parts by weight, mixing, and stirring until the mixture is uniformly dispersed to prepare an oil material;
the animal oil comprises at least one of fish oil, shrimp oil, pork, chicken, beef tallow and mutton fat;
the emulsifier comprises at least one of sucrose ester, polyglycerol stearate, monoglyceride, diglycerol fatty acid ester, tween and lecithin.
6. The method according to claim 1, wherein the S3 nutritional paste forming base is mixed by:
respectively heating a water system base material and an oil system material to 70-100 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1-0.5, homogenizing and emulsifying while adding until the system is uniform, forming a stable oil-in-water system, and obtaining the nutritional paste with uniform shape without free oil materials.
7. The method according to claim 6, wherein the homogeneous emulsification is shear homogeneous emulsification, and the rotation speed of the shear homogeneous emulsification is 1000-30000 rpm for 5-30 min.
8. The method of claim 1, further comprising S4 sterilizing, packaging: and (3) preserving the heat of the nutrient paste at 65-70 ℃ for sterilization for 15min, and then subpackaging according to the production requirement.
9. A pet nutrition paste prepared by the method of claim 1.
10. The nutritional paste according to claim 9, wherein the krill protein peptides are present in the nutritional paste at a mass fraction of no less than 10%.
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| 宣为民，等: "《正宗北方家常菜I 蒸》", 30 September 2008, 天津科学技术出版社 * |
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