CN112505239A - Titration end point automatic determination method for grain fatty acid value determination analyzer - Google Patents
Titration end point automatic determination method for grain fatty acid value determination analyzer Download PDFInfo
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- CN112505239A CN112505239A CN202010945473.4A CN202010945473A CN112505239A CN 112505239 A CN112505239 A CN 112505239A CN 202010945473 A CN202010945473 A CN 202010945473A CN 112505239 A CN112505239 A CN 112505239A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
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Abstract
The invention discloses an automatic titration end point judgment method for a grain fatty acid value determination analyzer, which is characterized by comprising the following steps of: the light emitted by the light source irradiates the color sensor after transmitting the grain sample extracting solution before titration in the titration cell, and the control system receives and stores an initial signal output by the color sensor; when the titration is started, the control system receives and stores a titrated signal output by the color sensor; and the control system automatically judges whether the titration end point is reached according to the G value difference value of the titrated signal and the initial signal. According to the invention, the G value is taken as a reference value, and the color sensor finally determines the experimental result by measuring the color difference before and after titration, so that the problem caused by inconsistent colors of the initial extracting solutions of different sample species is perfectly solved; the problems that different personnel titration results of primary assay personnel are large in difference and the personnel are not sensitive to observation before and after color change during detection, so that the experimental result is inaccurate and stable are solved.
Description
Technical Field
The invention belongs to the technical field of automatic titration end point determination methods for chemical analysis instruments, and particularly relates to an automatic titration end point determination method for a grain fatty acid value determination analyzer.
Background
At present, the principle of the grain fatty acid value measuring instrument is mainly that the grain fatty acid value measuring instrument is composed of three primary colors of red, green and blue according to the color, and the R value, the G value and the B value are corresponding to the primary colors. In the acid-base titration process, as the reaction proceeds, the three values change regularly, and the measuring instrument determines the result by setting the G value of the titration end point. Some problems occur in practical application, the initial colors of the sample extracting solutions of various grains are different, so that the titration end points of different samples are inconsistent in color, some samples are too light and some samples are too deep, and the data deviation ratio is large, so that the error of the detected fatty acid value of the grains is large.
Disclosure of Invention
The invention aims to provide an automatic titration end point judgment method for a grain fatty acid value determination analyzer, aiming at the defects of the prior art.
The technical scheme adopted by the invention is as follows: a titration end point automatic judging method for grain fatty acid value determination analyzer, light emitted by light source is irradiated on a color sensor after transmitting grain sample extract before titration in a titration cell, and a control system receives and stores initial signals output by the color sensor; when titration is started, light emitted by a light source irradiates a color sensor after transmitting reaction liquid in a titration cell, a control system receives and stores a signal after titration output by the color sensor, and an initial signal and a signal after titration output by the color sensor are G values of RGB color values of light rays penetrating through the titration cell and the reaction liquid; and the control system automatically judges whether the titration end point is reached according to the G value difference value of the titrated signal and the initial signal.
After adopting the structure, compared with the prior art, the invention has the advantages that: the invention is mainly applied to the detection of the storage quality of raw grains by grain storage and quality inspection departments, can effectively screen new grains and old grains, ensures the grain safety of the country, and finally determines the experimental result by taking the G value as a reference value and measuring the color difference before and after titration by the color sensor, thereby perfectly solving the problem caused by the inconsistent colors of the initial extracting solutions of different sample species; the detection result is more stable and accurate and cannot be achieved by manual titration; the problems that different staff titration results of primary laboratory test staff are large in difference, staff are not sensitive to observation before and after color change, professional skills of the primary laboratory test staff are not enough, and the experimental result is inaccurate and stable are solved well.
Detailed Description
The following description is only a preferred embodiment of the present invention, and does not limit the scope of the present invention, and the present invention will be further described with reference to the following embodiments.
In the embodiment, the automatic titration end point judging method for the grain fatty acid value measuring and analyzing instrument is characterized in that the grain fatty acid value measuring and analyzing instrument is in a working state, in order to start titration, light emitted by a light source irradiates a color sensor after transmitting grain sample extracting solution before titration in a titration cell, and a control system receives and stores initial signals output by the color sensor.
The titration is started, a standard solution is automatically dripped into the titration cell by a grain fatty acid value determination and analysis instrument, light emitted by a light source irradiates the color sensor after transmitting reaction liquid in the titration cell, the color sensor measures the color of the reaction liquid in the titration cell, and the control system receives and stores titrated signals output by the color sensor under different specific wavelengths, wherein the initial signals and the titrated signals output by the color sensor are G values of RGB color values of light rays which penetrate through the titration cell and the reaction liquid.
And the control system automatically judges whether the titration end point is reached according to the G value difference value of the titrated signal and the initial signal. If the titration end point is reached, the titration is finished; otherwise, the titration is continued until the titration end point is reached.
The invention is mainly applied to the detection of the storage quality of raw grains by grain storage and quality inspection departments, can effectively screen new grains and old grains, ensures the grain safety of the country, and finally determines the experimental result by taking the G value as a reference value and measuring the color difference before and after titration by the color sensor, thereby perfectly solving the problem caused by the inconsistent colors of the initial extracting solutions of different sample species; the detection result is more stable and accurate and cannot be achieved by manual titration; the problems that different staff titration results of primary laboratory test staff are large in difference, staff are not sensitive to observation before and after color change, professional skills of the primary laboratory test staff are not enough, and the experimental result is inaccurate and stable are solved well.
The following tables are the results of manual titration, titration of a prior art grain fatty acid value determination assay analyzer and prototype titration of the grain fatty acid value determination assay analyzer using the titration end point automatic determination method of the present invention:
table 1: results of manual titration (unit mg/100g)
| Japonica rice | Fatty acid value | Indica rice | Fatty acid value | Corn (corn) | Fatty acid value | Wheat flour | Fatty acid value |
| 1 | 13.2 | 1 | 15.3 | 1 | 20.1 | 1 | 15.3 |
| 2 | 15.6 | 2 | 16.5 | 2 | 46.5 | 2 | 20.4 |
| 3 | 18.7 | 3 | 20.3 | 3 | 16.8 | 3 | 40.6 |
| 4 | 27.8 | 4 | 30.6 | 4 | 36.4 | 4 | 35.4 |
| 5 | 38.2 | 5 | 45.9 | 5 | 49.7 | 5 | 48.7 |
Table 2: titration results in prior art instruments (unit mg/100g)
| Japonica rice | Fatty acid value | Indica rice | Fatty acid value | Corn (corn) | Fatty acid value | Wheat flour | Fatty acid value |
| 1 | 14.9 | 1 | 14.8 | 1 | 21.0 | 1 | 19.8 |
| 2 | 14.5 | 2 | 15.7 | 2 | 47.6 | 2 | 23.6 |
| 3 | 17.4 | 3 | 21.6 | 3 | -- | 3 | 41.0 |
| 4 | 29.3 | 4 | 30.1 | 4 | -- | 4 | 32.9 |
| 5 | 38.9 | 5 | 46.5 | 5 | -- | 5 | 40.7 |
- -represents a non-detectable result
Table 3: titration results (unit mg/100g) of model No. 1 of the invention
| Japonica rice | Fatty acid value | Indica rice | Fatty acid value | Corn (corn) | Fatty acid value | Wheat flour | Fatty acid value |
| 1 | 13.1 | 1 | 15.3 | 1 | 20.3 | 1 | 15.6 |
| 2 | 15.7 | 2 | 16.7 | 2 | 46.6 | 2 | 20.4 |
| 3 | 18.7 | 3 | 20.4 | 3 | 16.4 | 3 | 40.3 |
| 4 | 27.5 | 4 | 30.5 | 4 | 36.5 | 4 | 35.3 |
| 5 | 38.3 | 5 | 45.8 | 5 | 49.7 | 5 | 48.9 |
Table 4: titration results (unit mg/100g) of model No. 2 of the invention
| Japonica rice | Fatty acid value | Indica rice | Fatty acid value | Corn (corn) | Fatty acid value | Wheat flour | Fatty acid value |
| 1 | 13.1 | 1 | 15.2 | 1 | 20.4 | 1 | 15.4 |
| 2 | 15.6 | 2 | 16.6 | 2 | 46.2 | 2 | 20.7 |
| 3 | 18.9 | 3 | 20.5 | 3 | 16.4 | 3 | 40.4 |
| 4 | 27.7 | 4 | 30.2 | 4 | 36.5 | 4 | 35.6 |
| 5 | 38.1 | 5 | 45.5 | 5 | 50.0 | 5 | 48.6 |
Through tables 1, 3 and 4, the results of two prototype machines of the invention are very consistent with the results of manual titration, the accuracy completely meets the national standard requirements, and the error range is greatly superior to the 2mg error range specified by the national standard. As can be seen from the comparison between Table 1 and Table 2, the limit error of 1.7 is not more than 2 for the detection of rice, so the data of the prior art instrument is also valid, but the prior art instrument cannot detect the fatty acid value for the detection of white corn in corn, and the errors of the prior art instrument in the detection of the samples No. 1, No. 2 and No. 5 for five kinds of wheat flour with different whiteness exceed the range required by the national standard.
Then, the repeatability and the inter-platform difference of two sample machines are detected, four types of samples are respectively taken, each sample is continuously detected for 6 times, the two samples are simultaneously detected, and the experimental results are as follows:
table 5:
as seen in Table 5, the requirements of the two prototype machines on the repeatability limit of the samples meet GB/T20569-2006, GB/T20570-2015 and GB/T5510-2011 when the two prototype machines detect four types of samples. The difference between the two prototype machines is 0.1, the analysis reproducibility requirement of a common instrument is met, and the consistency of the two instruments is good.
Claims (1)
1. A titration end point automatic determination method for a grain fatty acid value determination analyzer is characterized in that: the light emitted by the light source irradiates the color sensor after transmitting the grain sample extracting solution before titration in the titration cell, and the control system receives and stores an initial signal output by the color sensor; when titration is started, light emitted by a light source irradiates a color sensor after transmitting reaction liquid in a titration cell, a control system receives and stores a signal after titration output by the color sensor, and an initial signal and a signal after titration output by the color sensor are G values of RGB color values of light rays penetrating through the titration cell and the reaction liquid; and the control system automatically judges whether the titration end point is reached according to the G value difference value of the titrated signal and the initial signal.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113945679A (en) * | 2021-10-22 | 2022-01-18 | 西安热工研究院有限公司 | Automatic detection method for acid value of synthetic lubricating oil |
| CN114019091A (en) * | 2021-11-09 | 2022-02-08 | 西安热工研究院有限公司 | Acid value detection method for synthetic lubricating oil |
| CN114577562A (en) * | 2022-01-28 | 2022-06-03 | 浙江伯利恒仪器设备有限公司 | High-frequency oscillation centrifugal fatty acid value measuring method |
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| CN102183520A (en) * | 2011-04-26 | 2011-09-14 | 东北电力大学 | Water hardness on-line measurement device based on solution image technology and measurement method thereof |
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| CN113945679A (en) * | 2021-10-22 | 2022-01-18 | 西安热工研究院有限公司 | Automatic detection method for acid value of synthetic lubricating oil |
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| CN114019091A (en) * | 2021-11-09 | 2022-02-08 | 西安热工研究院有限公司 | Acid value detection method for synthetic lubricating oil |
| CN114577562A (en) * | 2022-01-28 | 2022-06-03 | 浙江伯利恒仪器设备有限公司 | High-frequency oscillation centrifugal fatty acid value measuring method |
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