CN112504172A - Method for measuring plant leaf area suitable for field - Google Patents
Method for measuring plant leaf area suitable for field Download PDFInfo
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- CN112504172A CN112504172A CN202011480931.8A CN202011480931A CN112504172A CN 112504172 A CN112504172 A CN 112504172A CN 202011480931 A CN202011480931 A CN 202011480931A CN 112504172 A CN112504172 A CN 112504172A
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- measured
- blade
- plastic sheet
- transparent plastic
- area
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/28—Measuring arrangements characterised by the use of optical techniques for measuring areas
- G01B11/285—Measuring arrangements characterised by the use of optical techniques for measuring areas using photoelectric detection means
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- General Physics & Mathematics (AREA)
- Length Measuring Devices By Optical Means (AREA)
Abstract
The invention discloses a method suitable for measuring the leaf area of a plant in the field, which comprises the steps of fixing a hard transparent plastic sheet A on standard scale paper, and spreading a leaf to be measured on the surface of the hard transparent plastic sheet A; covering a hard transparent plastic sheet B on the surface of the blade to be detected; scanning standard calibration paper by using a handheld scanner; reading the pixel number of the leaf to be detected in the image obtained in the step three by utilizing Dizimizer image processing software, and calculating the pixel number of one unit area; dividing the number of pixels of the blade to be measured by the number of pixels in unit area to calculate the area of the blade to be measured; according to the invention, the leaf to be measured is placed on the standard scale paper for scanning, and then the measurement and calculation of the area of the leaf to be measured are carried out in a way of calculating pixels through Dizimizer image processing software, so that the whole operation process is simple, the obtained measurement result is relatively accurate, and a convenient and accurate measurement and calculation method is provided for the field plant leaf area measurement.
Description
Technical Field
The invention relates to the technical field of plant leaf area measurement, in particular to a method suitable for measuring plant leaf area in the field.
Background
When the growth process of plants is researched, the size of the leaf area is one of important indexes of the growth state of the plants, and the existing method for calculating the leaf area mainly adopts a multi-grid method and a drawing and paper weighing methodLeaf area meter method, etc.; the operation steps of the grid counting method are simple, but the operation process is more complicated, the workload of grid counting is larger, the calculation efficiency is low, and the grid counting method is not suitable for field operation; the area calculation error of the irregular blade and the small blade is large; the paper-drawing weighing method comprises drawing leaves on paper with uniform thickness, cutting into paper leaves, weighing on a balance, and cutting 3 pieces of paper with length of 10cm2The average mass is determined after the paper sheets are weighed, and the measured leaf area is equal to the weight of the paper leaves divided by 3 leaves with the length of 10cm2The average paper quality is multiplied by 100, the whole calculation process is complicated, the consumed time is long, and the method is not suitable for field operation; the leaf area measuring instrument has high cost and high price, the scanning speed during hand-held operation has influence on the measuring result, and the measurement of the slender small leaf is not very convenient and accurate. At present, researchers urgently need a method for rapidly collecting plant leaf area suitable for the field. In recent years, development and application of image processing software are becoming widespread, wherein the Dizimizer image processing software is a piece of software for measuring and analyzing images, and can manually and accurately measure the content of the images and perform automatic object recognition. In view of this, the application of the dzimizer image processing software to the calculation of the blade area is of great value for improving the calculation accuracy.
Disclosure of Invention
The invention aims to solve the technical problem of providing a measuring method for calculating the area of a blade by using Dizimizer image processing software.
Aiming at the technical problems, the method for measuring the leaf area of the plant in the field comprises the following steps:
fixing a hard transparent plastic sheet A on standard scale paper, and spreading a blade to be measured on the surface of the hard transparent plastic sheet A;
covering a hard transparent plastic sheet B on the surface of the blade to be measured;
thirdly, scanning standard scale paper by using a handheld scanner, and storing an image;
step four, reading the pixel number of the blade to be detected in the image obtained in the step three by utilizing Dizimizer image processing software;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, and calculating the number of the pixel points in unit area;
and step six, calculating the number of pixels of the blade to be measured in the acquired image by using the step four, and dividing the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured.
Further, the edges of the standard calibration paper and the hard transparent plastic sheet A are sealed by using a transparent adhesive tape.
Further, the hard transparent plastic sheet B is bonded and fixed to the edge of the hard transparent plastic sheet a.
Compared with the prior art, the invention has the following advantages:
according to the method, the leaves to be measured are placed on the standard scale paper to be scanned, and then the area of the leaves to be measured is measured and calculated in a pixel calculation mode through the Dizimizer image processing software, so that the leaf area images of slender leaves of plants can be rapidly collected in the field, the method is suitable for field operation, the whole operation process is simple, the obtained measurement result is relatively accurate, and the convenient and accurate measurement and calculation method is provided for field plant leaf area measurement.
Detailed Description
The technical scheme of the invention is further explained in detail by combining the specific embodiments as follows:
fixing a hard transparent plastic sheet A on standard scale paper, unfolding and flatly paving a blade to be measured on the surface of the hard transparent plastic sheet A, and sealing the edges of the standard scale paper and the hard transparent plastic sheet A by using a transparent adhesive tape, so that the standard scale paper is waterproof and dustproof, and the standard scale paper is prevented from being polluted to influence the measurement precision;
covering a hard transparent plastic sheet B on the surface of the blade to be measured, attaching and fixing the hard transparent plastic sheet B and the edge of the hard transparent plastic sheet A, flattening the blade to be measured, completely unfolding the blade to be measured, and obtaining a more accurate measurement result;
scanning standard scale paper by using a handheld scanner, storing an image, obtaining electronic pictures of the blade to be measured and the standard scale paper, and preparing for pixel measurement by using Dizimizer image processing software;
reading the pixel number of the blade to be measured in the image obtained in the step three by utilizing Dizimizer image processing software, and starting to measure the pixel number of the blade to be measured;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, calculating the number of the pixel points in unit area, and determining a reference standard;
step six, dividing the number of pixels of the blade to be measured in the image obtained in the step four by the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured; and finishing the calculation of the area of the blade to be measured according to the reference standard.
Claims (3)
1. A method suitable for measuring the leaf area of a plant in the field is characterized in that: the method for measuring the leaf area of the plant comprises the following steps:
firstly, fixing a hard transparent plastic sheet A on standard scale paper, and spreading a blade to be measured on the surface of the hard transparent plastic sheet A;
covering a hard transparent plastic sheet B on the surface of the blade to be measured;
thirdly, scanning the standard scale paper by using a handheld scanner, and storing an image;
reading the number of pixels of the blade to be detected in the image obtained in the step three by utilizing Dizimizer image processing software;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, and calculating the number of the pixel points in unit area;
and step six, calculating the number of pixels of the blade to be measured in the acquired image by using the step four, and dividing the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured.
2. The method for measuring the leaf area of the plant in the field according to claim 1, wherein the method comprises the following steps: and the standard scale paper and the edge of the hard transparent plastic sheet A are sealed by using a transparent adhesive tape.
3. The method for measuring the leaf area of the plant in the field according to claim 1, wherein the method comprises the following steps: and the hard transparent plastic sheet B is fixedly attached to the edge of the hard transparent plastic sheet A.
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CN202011480931.8A CN112504172A (en) | 2020-12-15 | 2020-12-15 | Method for measuring plant leaf area suitable for field |
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CN202011480931.8A CN112504172A (en) | 2020-12-15 | 2020-12-15 | Method for measuring plant leaf area suitable for field |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113624165A (en) * | 2021-08-09 | 2021-11-09 | 海南青峰生物科技有限公司 | Rice leaf area measuring device and method based on 5G and image recognition |
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Application publication date: 20210316 |