CN112504172A - Method for measuring plant leaf area suitable for field - Google Patents
Method for measuring plant leaf area suitable for field Download PDFInfo
- Publication number
- CN112504172A CN112504172A CN202011480931.8A CN202011480931A CN112504172A CN 112504172 A CN112504172 A CN 112504172A CN 202011480931 A CN202011480931 A CN 202011480931A CN 112504172 A CN112504172 A CN 112504172A
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- leaf
- plastic sheet
- transparent plastic
- area
- measured
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 229920003023 plastic Polymers 0.000 claims abstract description 21
- 239000002985 plastic film Substances 0.000 claims abstract description 21
- 238000005259 measurement Methods 0.000 claims abstract description 12
- 238000004364 calculation method Methods 0.000 abstract description 10
- 238000000691 measurement method Methods 0.000 abstract description 2
- 238000005303 weighing Methods 0.000 description 3
- 239000002390 adhesive tape Substances 0.000 description 2
- 230000001788 irregular Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/28—Measuring arrangements characterised by the use of optical techniques for measuring areas
- G01B11/285—Measuring arrangements characterised by the use of optical techniques for measuring areas using photoelectric detection means
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Length Measuring Devices By Optical Means (AREA)
Abstract
本发明公开的一种适用于野外测量植物叶面积的方法，在标准刻度纸上固定硬质透明塑料片A，将待测叶片展开平铺于硬质透明塑料片A表面；在待测叶片表面覆盖硬质透明塑料片B；用手持式扫描仪扫描标准刻度纸；利用Dizimizer图像处理软件读取步骤三图像中待测叶片的像素数量，并计算出一个单位面积的像素点数量；用待测叶片的像素数量，除以单位面积的像素点数量，计算出待测叶片的面积；本发明将待测叶片置于标准刻度纸上进行扫描，之后通过Dizimizer图像处理软件计算像素的方式进行待测叶片面积的测量计算，整个操作过程简单，获得的测量结果也较为精确，为野外进行植物叶片面积测量提供了一种便捷、精确的测量计算方法。The invention discloses a method suitable for measuring the leaf area of plants in the field. A rigid transparent plastic sheet A is fixed on a standard scale paper, and the leaves to be measured are spread out on the surface of the rigid transparent plastic sheet A; Cover the hard transparent plastic sheet B; scan the standard scale paper with a handheld scanner; use the Dizimizer image processing software to read the number of pixels of the leaf to be tested in the image in step 3, and calculate the number of pixels per unit area; The number of pixels of the leaf is divided by the number of pixels per unit area to calculate the area of the leaf to be measured; in the present invention, the leaf to be measured is placed on standard scale paper for scanning, and then the pixel to be measured is calculated by the Dizimizer image processing software. For the measurement and calculation of leaf area, the entire operation process is simple, and the obtained measurement results are relatively accurate, which provides a convenient and accurate measurement and calculation method for the field measurement of plant leaf area.
Description
Technical Field
The invention relates to the technical field of plant leaf area measurement, in particular to a method suitable for measuring plant leaf area in the field.
Background
When the growth process of plants is researched, the size of the leaf area is one of important indexes of the growth state of the plants, and the existing method for calculating the leaf area mainly adopts a multi-grid method and a drawing and paper weighing methodLeaf area meter method, etc.; the operation steps of the grid counting method are simple, but the operation process is more complicated, the workload of grid counting is larger, the calculation efficiency is low, and the grid counting method is not suitable for field operation; the area calculation error of the irregular blade and the small blade is large; the paper-drawing weighing method comprises drawing leaves on paper with uniform thickness, cutting into paper leaves, weighing on a balance, and cutting 3 pieces of paper with length of 10cm2The average mass is determined after the paper sheets are weighed, and the measured leaf area is equal to the weight of the paper leaves divided by 3 leaves with the length of 10cm2The average paper quality is multiplied by 100, the whole calculation process is complicated, the consumed time is long, and the method is not suitable for field operation; the leaf area measuring instrument has high cost and high price, the scanning speed during hand-held operation has influence on the measuring result, and the measurement of the slender small leaf is not very convenient and accurate. At present, researchers urgently need a method for rapidly collecting plant leaf area suitable for the field. In recent years, development and application of image processing software are becoming widespread, wherein the Dizimizer image processing software is a piece of software for measuring and analyzing images, and can manually and accurately measure the content of the images and perform automatic object recognition. In view of this, the application of the dzimizer image processing software to the calculation of the blade area is of great value for improving the calculation accuracy.
Disclosure of Invention
The invention aims to solve the technical problem of providing a measuring method for calculating the area of a blade by using Dizimizer image processing software.
Aiming at the technical problems, the method for measuring the leaf area of the plant in the field comprises the following steps:
fixing a hard transparent plastic sheet A on standard scale paper, and spreading a blade to be measured on the surface of the hard transparent plastic sheet A;
covering a hard transparent plastic sheet B on the surface of the blade to be measured;
thirdly, scanning standard scale paper by using a handheld scanner, and storing an image;
step four, reading the pixel number of the blade to be detected in the image obtained in the step three by utilizing Dizimizer image processing software;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, and calculating the number of the pixel points in unit area;
and step six, calculating the number of pixels of the blade to be measured in the acquired image by using the step four, and dividing the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured.
Further, the edges of the standard calibration paper and the hard transparent plastic sheet A are sealed by using a transparent adhesive tape.
Further, the hard transparent plastic sheet B is bonded and fixed to the edge of the hard transparent plastic sheet a.
Compared with the prior art, the invention has the following advantages:
according to the method, the leaves to be measured are placed on the standard scale paper to be scanned, and then the area of the leaves to be measured is measured and calculated in a pixel calculation mode through the Dizimizer image processing software, so that the leaf area images of slender leaves of plants can be rapidly collected in the field, the method is suitable for field operation, the whole operation process is simple, the obtained measurement result is relatively accurate, and the convenient and accurate measurement and calculation method is provided for field plant leaf area measurement.
Detailed Description
The technical scheme of the invention is further explained in detail by combining the specific embodiments as follows:
fixing a hard transparent plastic sheet A on standard scale paper, unfolding and flatly paving a blade to be measured on the surface of the hard transparent plastic sheet A, and sealing the edges of the standard scale paper and the hard transparent plastic sheet A by using a transparent adhesive tape, so that the standard scale paper is waterproof and dustproof, and the standard scale paper is prevented from being polluted to influence the measurement precision;
covering a hard transparent plastic sheet B on the surface of the blade to be measured, attaching and fixing the hard transparent plastic sheet B and the edge of the hard transparent plastic sheet A, flattening the blade to be measured, completely unfolding the blade to be measured, and obtaining a more accurate measurement result;
scanning standard scale paper by using a handheld scanner, storing an image, obtaining electronic pictures of the blade to be measured and the standard scale paper, and preparing for pixel measurement by using Dizimizer image processing software;
reading the pixel number of the blade to be measured in the image obtained in the step three by utilizing Dizimizer image processing software, and starting to measure the pixel number of the blade to be measured;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, calculating the number of the pixel points in unit area, and determining a reference standard;
step six, dividing the number of pixels of the blade to be measured in the image obtained in the step four by the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured; and finishing the calculation of the area of the blade to be measured according to the reference standard.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011480931.8A CN112504172A (en) | 2020-12-15 | 2020-12-15 | Method for measuring plant leaf area suitable for field |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011480931.8A CN112504172A (en) | 2020-12-15 | 2020-12-15 | Method for measuring plant leaf area suitable for field |
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| Publication Number | Publication Date |
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| CN112504172A true CN112504172A (en) | 2021-03-16 |
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| CN202011480931.8A Pending CN112504172A (en) | 2020-12-15 | 2020-12-15 | Method for measuring plant leaf area suitable for field |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113624165A (en) * | 2021-08-09 | 2021-11-09 | 海南青峰生物科技有限公司 | Rice leaf area measuring device and method based on 5G and image recognition |
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2020
- 2020-12-15 CN CN202011480931.8A patent/CN112504172A/en active Pending
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Application publication date: 20210316 |
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