CN112504172A - Method for measuring plant leaf area suitable for field - Google Patents

Method for measuring plant leaf area suitable for field Download PDF

Info

Publication number
CN112504172A
CN112504172A CN202011480931.8A CN202011480931A CN112504172A CN 112504172 A CN112504172 A CN 112504172A CN 202011480931 A CN202011480931 A CN 202011480931A CN 112504172 A CN112504172 A CN 112504172A
Authority
CN
China
Prior art keywords
measured
blade
plastic sheet
transparent plastic
area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202011480931.8A
Other languages
Chinese (zh)
Inventor
刘颖
王彦龙
马玉寿
李世雄
王晓丽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qinghai University
Original Assignee
Qinghai University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qinghai University filed Critical Qinghai University
Priority to CN202011480931.8A priority Critical patent/CN112504172A/en
Publication of CN112504172A publication Critical patent/CN112504172A/en
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B11/00Measuring arrangements characterised by the use of optical techniques
    • G01B11/28Measuring arrangements characterised by the use of optical techniques for measuring areas
    • G01B11/285Measuring arrangements characterised by the use of optical techniques for measuring areas using photoelectric detection means

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Length Measuring Devices By Optical Means (AREA)

Abstract

The invention discloses a method suitable for measuring the leaf area of a plant in the field, which comprises the steps of fixing a hard transparent plastic sheet A on standard scale paper, and spreading a leaf to be measured on the surface of the hard transparent plastic sheet A; covering a hard transparent plastic sheet B on the surface of the blade to be detected; scanning standard calibration paper by using a handheld scanner; reading the pixel number of the leaf to be detected in the image obtained in the step three by utilizing Dizimizer image processing software, and calculating the pixel number of one unit area; dividing the number of pixels of the blade to be measured by the number of pixels in unit area to calculate the area of the blade to be measured; according to the invention, the leaf to be measured is placed on the standard scale paper for scanning, and then the measurement and calculation of the area of the leaf to be measured are carried out in a way of calculating pixels through Dizimizer image processing software, so that the whole operation process is simple, the obtained measurement result is relatively accurate, and a convenient and accurate measurement and calculation method is provided for the field plant leaf area measurement.

Description

Method for measuring plant leaf area suitable for field
Technical Field
The invention relates to the technical field of plant leaf area measurement, in particular to a method suitable for measuring plant leaf area in the field.
Background
When the growth process of plants is researched, the size of the leaf area is one of important indexes of the growth state of the plants, and the existing method for calculating the leaf area mainly adopts a multi-grid method and a drawing and paper weighing methodLeaf area meter method, etc.; the operation steps of the grid counting method are simple, but the operation process is more complicated, the workload of grid counting is larger, the calculation efficiency is low, and the grid counting method is not suitable for field operation; the area calculation error of the irregular blade and the small blade is large; the paper-drawing weighing method comprises drawing leaves on paper with uniform thickness, cutting into paper leaves, weighing on a balance, and cutting 3 pieces of paper with length of 10cm2The average mass is determined after the paper sheets are weighed, and the measured leaf area is equal to the weight of the paper leaves divided by 3 leaves with the length of 10cm2The average paper quality is multiplied by 100, the whole calculation process is complicated, the consumed time is long, and the method is not suitable for field operation; the leaf area measuring instrument has high cost and high price, the scanning speed during hand-held operation has influence on the measuring result, and the measurement of the slender small leaf is not very convenient and accurate. At present, researchers urgently need a method for rapidly collecting plant leaf area suitable for the field. In recent years, development and application of image processing software are becoming widespread, wherein the Dizimizer image processing software is a piece of software for measuring and analyzing images, and can manually and accurately measure the content of the images and perform automatic object recognition. In view of this, the application of the dzimizer image processing software to the calculation of the blade area is of great value for improving the calculation accuracy.
Disclosure of Invention
The invention aims to solve the technical problem of providing a measuring method for calculating the area of a blade by using Dizimizer image processing software.
Aiming at the technical problems, the method for measuring the leaf area of the plant in the field comprises the following steps:
fixing a hard transparent plastic sheet A on standard scale paper, and spreading a blade to be measured on the surface of the hard transparent plastic sheet A;
covering a hard transparent plastic sheet B on the surface of the blade to be measured;
thirdly, scanning standard scale paper by using a handheld scanner, and storing an image;
step four, reading the pixel number of the blade to be detected in the image obtained in the step three by utilizing Dizimizer image processing software;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, and calculating the number of the pixel points in unit area;
and step six, calculating the number of pixels of the blade to be measured in the acquired image by using the step four, and dividing the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured.
Further, the edges of the standard calibration paper and the hard transparent plastic sheet A are sealed by using a transparent adhesive tape.
Further, the hard transparent plastic sheet B is bonded and fixed to the edge of the hard transparent plastic sheet a.
Compared with the prior art, the invention has the following advantages:
according to the method, the leaves to be measured are placed on the standard scale paper to be scanned, and then the area of the leaves to be measured is measured and calculated in a pixel calculation mode through the Dizimizer image processing software, so that the leaf area images of slender leaves of plants can be rapidly collected in the field, the method is suitable for field operation, the whole operation process is simple, the obtained measurement result is relatively accurate, and the convenient and accurate measurement and calculation method is provided for field plant leaf area measurement.
Detailed Description
The technical scheme of the invention is further explained in detail by combining the specific embodiments as follows:
fixing a hard transparent plastic sheet A on standard scale paper, unfolding and flatly paving a blade to be measured on the surface of the hard transparent plastic sheet A, and sealing the edges of the standard scale paper and the hard transparent plastic sheet A by using a transparent adhesive tape, so that the standard scale paper is waterproof and dustproof, and the standard scale paper is prevented from being polluted to influence the measurement precision;
covering a hard transparent plastic sheet B on the surface of the blade to be measured, attaching and fixing the hard transparent plastic sheet B and the edge of the hard transparent plastic sheet A, flattening the blade to be measured, completely unfolding the blade to be measured, and obtaining a more accurate measurement result;
scanning standard scale paper by using a handheld scanner, storing an image, obtaining electronic pictures of the blade to be measured and the standard scale paper, and preparing for pixel measurement by using Dizimizer image processing software;
reading the pixel number of the blade to be measured in the image obtained in the step three by utilizing Dizimizer image processing software, and starting to measure the pixel number of the blade to be measured;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, calculating the number of the pixel points in unit area, and determining a reference standard;
step six, dividing the number of pixels of the blade to be measured in the image obtained in the step four by the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured; and finishing the calculation of the area of the blade to be measured according to the reference standard.

Claims (3)

1. A method suitable for measuring the leaf area of a plant in the field is characterized in that: the method for measuring the leaf area of the plant comprises the following steps:
firstly, fixing a hard transparent plastic sheet A on standard scale paper, and spreading a blade to be measured on the surface of the hard transparent plastic sheet A;
covering a hard transparent plastic sheet B on the surface of the blade to be measured;
thirdly, scanning the standard scale paper by using a handheld scanner, and storing an image;
reading the number of pixels of the blade to be detected in the image obtained in the step three by utilizing Dizimizer image processing software;
step five, reading the number of a row of pixel points in unit length on the standard calibration paper by utilizing Dizimizer image processing software, and calculating the number of the pixel points in unit area;
and step six, calculating the number of pixels of the blade to be measured in the acquired image by using the step four, and dividing the number of pixels of one unit area calculated in the step five to calculate the area of the blade to be measured.
2. The method for measuring the leaf area of the plant in the field according to claim 1, wherein the method comprises the following steps: and the standard scale paper and the edge of the hard transparent plastic sheet A are sealed by using a transparent adhesive tape.
3. The method for measuring the leaf area of the plant in the field according to claim 1, wherein the method comprises the following steps: and the hard transparent plastic sheet B is fixedly attached to the edge of the hard transparent plastic sheet A.
CN202011480931.8A 2020-12-15 2020-12-15 Method for measuring plant leaf area suitable for field Pending CN112504172A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011480931.8A CN112504172A (en) 2020-12-15 2020-12-15 Method for measuring plant leaf area suitable for field

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011480931.8A CN112504172A (en) 2020-12-15 2020-12-15 Method for measuring plant leaf area suitable for field

Publications (1)

Publication Number Publication Date
CN112504172A true CN112504172A (en) 2021-03-16

Family

ID=74972057

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011480931.8A Pending CN112504172A (en) 2020-12-15 2020-12-15 Method for measuring plant leaf area suitable for field

Country Status (1)

Country Link
CN (1) CN112504172A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113624165A (en) * 2021-08-09 2021-11-09 海南青峰生物科技有限公司 Rice leaf area measuring device and method based on 5G and image recognition

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101298986A (en) * 2007-04-30 2008-11-05 中国科学院沈阳应用生态研究所 Field measuring method of sand broad leaf plant leaf area
CN101813469A (en) * 2010-04-23 2010-08-25 中国科学院成都生物研究所 Method for measuring leaf area by manually shooting
CN201811723U (en) * 2010-09-29 2011-04-27 中国烟草总公司郑州烟草研究院 Tobacco leaf blade area measuring system
CN102506772A (en) * 2011-11-01 2012-06-20 西北农林科技大学 Method and device for quickly detecting area of leaf blade based on mobile phone
CN103033151A (en) * 2012-12-24 2013-04-10 南京信息工程大学 Leaf area measuring method based on lasers and images
CN103791861A (en) * 2014-02-10 2014-05-14 黑龙江省森林与环境科学研究院 Plant leaf area lossless measuring method
CN104457627A (en) * 2014-12-18 2015-03-25 云南省农业科学院蚕桑蜜蜂研究所 Method for utilizing Photoshop for precisely measuring area of plane of irregular object
CN105136070A (en) * 2015-08-21 2015-12-09 上海植物园 Method for utilizing electronic device to measure plant leaf area
CN105180850A (en) * 2015-07-15 2015-12-23 中国农业大学 Method for calculating corn leaf exserohilum turcicum disease spot area ratio and device
CN106996757A (en) * 2017-04-18 2017-08-01 广西壮族自治区农业科学院农业资源与环境研究所 A kind of leaf area measurement method based on digital camera images
CN107024187A (en) * 2017-04-18 2017-08-08 广西壮族自治区农业科学院农业资源与环境研究所 A kind of leaf area measurement method based on digital camera images
CN107144244A (en) * 2017-05-16 2017-09-08 广西壮族自治区农业科学院农业资源与环境研究所 A kind of leaf area measurement method
CN109520447A (en) * 2018-11-29 2019-03-26 中国科学院南京地理与湖泊研究所 A method of amendment image treating measures hydrilla verticillata blade area

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101298986A (en) * 2007-04-30 2008-11-05 中国科学院沈阳应用生态研究所 Field measuring method of sand broad leaf plant leaf area
CN101813469A (en) * 2010-04-23 2010-08-25 中国科学院成都生物研究所 Method for measuring leaf area by manually shooting
CN201811723U (en) * 2010-09-29 2011-04-27 中国烟草总公司郑州烟草研究院 Tobacco leaf blade area measuring system
CN102506772A (en) * 2011-11-01 2012-06-20 西北农林科技大学 Method and device for quickly detecting area of leaf blade based on mobile phone
CN103033151A (en) * 2012-12-24 2013-04-10 南京信息工程大学 Leaf area measuring method based on lasers and images
CN103791861A (en) * 2014-02-10 2014-05-14 黑龙江省森林与环境科学研究院 Plant leaf area lossless measuring method
CN104457627A (en) * 2014-12-18 2015-03-25 云南省农业科学院蚕桑蜜蜂研究所 Method for utilizing Photoshop for precisely measuring area of plane of irregular object
CN105180850A (en) * 2015-07-15 2015-12-23 中国农业大学 Method for calculating corn leaf exserohilum turcicum disease spot area ratio and device
CN105136070A (en) * 2015-08-21 2015-12-09 上海植物园 Method for utilizing electronic device to measure plant leaf area
CN106996757A (en) * 2017-04-18 2017-08-01 广西壮族自治区农业科学院农业资源与环境研究所 A kind of leaf area measurement method based on digital camera images
CN107024187A (en) * 2017-04-18 2017-08-08 广西壮族自治区农业科学院农业资源与环境研究所 A kind of leaf area measurement method based on digital camera images
CN107144244A (en) * 2017-05-16 2017-09-08 广西壮族自治区农业科学院农业资源与环境研究所 A kind of leaf area measurement method
CN109520447A (en) * 2018-11-29 2019-03-26 中国科学院南京地理与湖泊研究所 A method of amendment image treating measures hydrilla verticillata blade area

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113624165A (en) * 2021-08-09 2021-11-09 海南青峰生物科技有限公司 Rice leaf area measuring device and method based on 5G and image recognition

Similar Documents

Publication Publication Date Title
CN101713641B (en) Digital graphic technology-based method and device for analyzing leaf area
CN201413131Y (en) Novel device for measuring and calculating leaf area index
CN103033151A (en) Leaf area measuring method based on lasers and images
CN103791861A (en) Plant leaf area lossless measuring method
CN104776814A (en) Rice leaf shape acquisition device and rice leaf shape parameter extraction method
CN112504172A (en) Method for measuring plant leaf area suitable for field
CN110068277A (en) A kind of system and method for automatic Observation crops plant height data
CN101799283A (en) Nondestructive measuring method for leaf area of broad leaved plant
CN202329577U (en) In-vivo measurement device for leaf area of broad-leaved plant
CN102914620A (en) Determination method for ration of reconstituted tobacco finished product
CN109030755A (en) The detection device and method of grain moisture content, mass of 1000 kernel and bulk density based on image processing techniques
CN104006784A (en) Precision measurement device for tree walk increment
CN110689567A (en) Method for measuring and calculating total leaf area of whole arbor
CN100344948C (en) Aviation high spectrum remote sensing flight ground synchronous scaling and reflectivity conversion method
CN111882569A (en) Machine vision-based pointer type pressure gauge automatic calibrator indication value reading method
CN106023235B (en) A kind of method that the effective seed number of crops measures
CN205538538U (en) Papermaking method reconstituted tobacco product ration spot test appearance
CN207249695U (en) A kind of fish character measuring device
CN108020656A (en) A kind of device and method for measuring rice embryo and being irradiated by heavy ion radiation
CN203163698U (en) Plant leaf thickness measurement device based on image processing
CN112378313A (en) Special calibration scale and method for multi-target digital image detection
CN203178021U (en) Pressure measuring system based on compensated-type micromanometer
CN112461075A (en) Grassland vegetation leaf area measuring tool and method
CN208140048U (en) leaf area index measuring device
CN206399324U (en) A kind of bristles percentage composition detection means

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20210316