CN112481153A - Rhodoblast sphagnola coupling complex microbial inoculum and application thereof - Google Patents
Rhodoblast sphagnola coupling complex microbial inoculum and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a Rhodoblastus sphagnicola coupling complex microbial inoculum and application thereof in degrading starch wastewater, according to the water quality characteristics of the starch wastewater, the ecological potential differentiation and metabolism complementation characteristics of Rhodoblastus sphagnicola PNSB-MHW and Bacillus atrophaeus are utilized, liquid seeds are prepared by optimizing the proportion of the Rhodoblastus sphagnicola PNSB-MHW and the Bacillus atrophaeus, the finally obtained complex microbial inoculum is inoculated into the starch wastewater according to the mass-volume ratio of 15%, the COD, ammonia nitrogen, total phosphorus, mercaptan, hydrogen sulfide and the like of the starch wastewater are obviously reduced within 144h, the degradation efficiency is more than 98%, and the degraded wastewater realizes the self-flocculation function of a bacterial strain, so that the complicated steps of acid-base regulation, flocculation precipitation and the like in wastewater treatment are simplified. The degradation characteristic of the Rhodoblast sphagnola PNSB-MHW coupled composite microbial inoculum is utilized, and the Rhodoblast sphagnola PNSB-MHW coupled composite microbial inoculum has wide application value in the technical field of microbial strain application.
Description
Technical Field
The invention relates to the technical field of microorganisms and application thereof, in particular to a Rhodoblast sphagna coupling complex microbial inoculum and the technical field of application thereof in starch wastewater degradation.
Background
China adopts an open production process or a semi-closed production process, so that the numerical values of COD, BOD5, SS and the like in potato starch wastewater in China are higher. The wastewater generated in the production of the corn starch is discharged, so that the starch cost is high, the starch consumes much water, the corn loss is large, and the serious environmental pollution is caused by the putrefaction and sulfurous acid residue of organic matters such as protein, fat and the like in the wastewater.
The purpose of starch wastewater treatment is to remove pollutants in wastewater and enable various indexes of the treated wastewater to reach the discharge standard. The existing starch wastewater treatment method comprises a physical method; a chemical method; a physical-chemical method; biochemical method; physical and biological methods; an enzymatic method. Wherein the biological treatment of the wastewater provides suitable conditions, and organic pollutants in a dissolved or colloidal state in the wastewater are degraded and converted into useful substances by using the metabolism of microorganisms, so that the wastewater is earned. Compared with physical and chemical treatment methods of wastewater, such as adsorption and coagulation, the methods only transfer organic matters from the wastewater, and need to consider subsequent treatment, and do not achieve treatment of both symptoms and root causes. The biological treatment method is a relatively thorough degradation of organic matters, so the biological treatment method is more and more emphasized and is also a main way for treating wastewater. Another method for applying more photosynthetic bacteria in the starch wastewater treatment method is to apply the photosynthetic bacteria cynanchum, organic matter degrading photosynthetic bacteria for short are a general name of bacteria which can perform photosynthesis under anaerobic conditions but do not release oxygen, the photosynthetic bacteria for purifying the organic wastewater are mainly rhodopseudomonas, the organic matters are degraded by utilizing the photosynthesis, the important action of the photosynthetic bacteria is disclosed by experimental researches of the Xiaolingzhengtai and the like in Japan in the seventy years of the century, and then people begin to apply the photosynthetic bacteria to treat high-concentration organic wastewater in large quantity. The photosynthetic bacteria sewage treatment method has the advantages of simple process, low treatment cost, stable operation and no secondary pollution, and becomes one of the first-choice strains for treating organic wastewater by a biological method. At present, although the photosynthetic bacteria sewage treatment method is applied to various organic wastewater treatments, only a few of the photosynthetic bacteria sewage treatment methods enter industrialized operation, and a great deal of work is still in the experimental research stage. In addition, the photosynthetic bacteria wastewater treatment also has the problems of poor reaction system stability, treatment limit of treated water, incapability of directly degrading macromolecular substances, difficult thallus sedimentation and the like, so that the application of the photosynthetic bacteria in the wastewater is still to be further researched.
Bacillus atrophaeus (Bacillus atrophaeus) nonpathogenic bacteria, a type of variant of Bacillus subtilis, form soluble black colonies on carbohydrate-containing media, primarily isolated from soil. The starch waste water degradation agent has wide application in the fields of agriculture, industry, scientific research, medical treatment, health and the like, and the application of the starch waste water degradation agent in the aspect of starch waste water degradation is not reported.
Disclosure of Invention
Aiming at the problems that the photosynthetic bacteria in the prior art have poor reaction system stability in the starch wastewater degradation aspect, treatment limit exists in starch wastewater treatment, macromolecular substances cannot be directly degraded, the bacteria are difficult to settle and the like, and the application of coupling the photosynthetic bacteria and the bacillus atrophaeus in the starch wastewater degradation aspect is not reported in documents. The invention aims to provide a new bacterium Rhodoblast sphagnola coupling complex bacterium and application thereof in degrading starch wastewater. The invention relates to a Rhodoblastus sphagnoides PNSB-MHW belonging to acidophilic photosynthetic bacteria and separated from acidic wastewater sludge in a plum blossom monosodium glutamate drainage ditch in five ditches in Xinjiang, which is compounded with Bacillus atrophaeus BA-BC separated from saline-alkali soil in Akebia city in Xinjiang, the Rhodoblastus sphagnoides PNSB-MHW and the Bacillus atrophaeus BA-BC are mixed according to the characteristics of ecological site differentiation and metabolism complementation of the photosynthetic bacteria and the Bacillus atrophaeus, the Rhodoblastus sphagnicus PNSB-MHW and the Bacillus atrophaeus BA-BC are mixed according to the volume ratio of 10:1 to obtain Rhodoblastus sphagnoides coupling composite microbial inoculum, the Rhodoblastus sphagnoides coupling composite microbial inoculum is inoculated into starch wastewater according to the mass-volume ratio of 15%, starch wastewater COD, ammonia nitrogen, total phosphorus, mercaptan, hydrogen sulfide and the like in 144h are all obviously reduced, the degradation efficiency is all more than 98%, the wastewater after degradation can realize the bacterial strain flocculation and flocculation adjustment of wastewater, and the complicated wastewater treatment can be realized, flocculation precipitation and other steps, and has wide value for expanding the application field of microbial strains.
The invention specifically provides a novel bacterium Rhodoblast sphagnola coupling complex microbial inoculum, which is prepared by compounding Rhodoblast sphagnola PNSB-MHW and Bacillus atrophaeus (Bacillus atrophaeus) in proportion.
In the invention, Rhodoblast sphagnola PNSB-MHW is screened and separated from plum blossom monosodium glutamate acidic wastewater blowdown water samples from five canals of Xinjiang, and is verified to belong to a typical new strain in the category of obtained acidophilic photosynthetic bacteria through well-known and accepted strain system molecular level identification and strain physiological and biochemical system tests, wherein the Rhodoblast sphagnola PNSB-MHW is preserved in the common microorganism center of China Committee for culture Collection with the preservation number: CGMCC No.20882, preservation date: 10 and 13 days in 2020.
The gene sequence of the Rhodoblast sphagnola PNSB-MHW CGMCC No:20882 strain is shown in SEQ ID NO: 1.
The Bacillus atrophaeus (Bacillus atrophaeus) BA-BC in the coupling composite bacterial agent is verified to belong to the Bacillus atrophaeus (Bacillus atrophaeus) commonly seen in the field through well-known and accepted molecular level identification of a bacterial system and tests and verification of a physiological and biochemical system of the bacterial, and persons skilled in the field can purchase and obtain the Bacillus atrophaeus through public channels, and the disclosed Bacillus atrophaeus (Bacillus atrophaeus) bacterial culture medium and culture method are suitable for implementation of the invention.
In the invention, the viable count of Rhodoblast sphagna PNSB-MHW and Bacillus atrophaeus in the coupled composite microbial inoculum is more than or equal to 109cfu/mL, Rhodoblast sphagna PNSB-MHW was formulated with Bacillus atrophaeus (Bacillus atrophaeus) at a volume ratio of 10: 1.
In the present invention, Rhodoblast sphagnola PNSB-MHW enrichment medium: KH (Perkin Elmer)2PO40.04g/L,NH4Cl 0.1g/L,MgCl2﹒6H2O 0.01g/L,CaCl2﹒2H2O 0.05g/L,CH3COONa1.0g/L, microelement mother liquor 1ml, vitamin complex mother liquor 1ml, pH 5.0.
In the present invention, Rhodoblast sphagnola PNSB-MHW separation Medium (RCVBN) (g/L): KH (Perkin Elmer)2PO4 0.5g/L,K2HPO4 0.3g/L，NH4Cl 1.0g/L,MgCl2﹒6H2O 0.2g/L,CaCl2﹒2H2O 0.5g/L,CH3COONa 1.0g/L,CH3CH2COONa 0.5g/L, yeast powder 0.1g/L, agar 7.0g/L, microelement mother liquor 1ml, and pH 5.0.
Meanwhile, the invention provides a preparation method of the novel rhodoblast sphagna coupling complex microbial inoculum, which comprises the following steps:
(1) inoculating the preserved strain Rhodoblast sphagnicola PNSB-MHW CGMCC No:20882 into a liquid modified Nisson & Dundas culture medium, performing static culture in an illumination culture box with illumination intensity of 1000-; inoculating Bacillus atrophaeus into liquid self-grinding culture medium, and culturing in a shaker at 30 deg.C and rotation speed of 120rad/min for 3-5d to stationary phase, wherein the thallus concentration contains 15g dry bacteria per liter.
(2) And (2) taking the two bacterial liquids cultured in the step (1), respectively carrying out centrifugal separation at room temperature, discarding the supernatant, precipitating, fully suspending with sterile water, centrifuging again, repeating the step for 3-5 times, and suspending the precipitate with sterile water to obtain two bacterial strain liquid seeds.
(3) And (3) respectively compounding the Rhodoblastus sphagnella PNSB-MHW obtained in the step (2) and liquid seeds of Bacillus atrophaeus according to a volume ratio under an aseptic condition to prepare the Rhodoblastus sphagnella coupling compound microbial inoculum.
Further, the invention provides application of the novel bacterium Rhodoblast sphagnicola coupling complex bacterium agent in degrading starch wastewater.
Specifically, the invention provides application of a new bacterium Rhodoblast sphagnicola coupling complex bacterium agent in degrading starch wastewater. The Rhodoblast sphagnicola coupled composite microbial inoculum is inoculated in starch wastewater according to the inoculation amount of 15% by mass volume, the anaerobic culture is carried out for 72h at 30 ℃ under illumination, then anaerobic illumination-dark aerobic alternate culture is carried out for 72h, and the wastewater degradation process is finished after the bacteria are completely self-flocculated and precipitated after standing for 24 h.
By implementing the technical scheme provided by the invention, the invention can obtain the following beneficial effects:
(1) the Rhodoblast sphagna PNSB-MHW strain provided by the invention is verified by well-known and recognized molecular level identification of a strain system and physiological and biochemical system tests of the strain in the field, and the obtained PNSB-MHW strain number in the acidophilic photosynthetic bacteria category is proved to belong to a typical new strain, so that the strain needs to be preserved according to legal requirements.
(2) The invention adopts a new bacterium, namely, the Rhodoblastus sphagnicola coupling complex bacterium agent, wherein a coupling reaction system of the Rhodoblastus sphagnicola PNSB-MHW and Bacillus atrophaeus is stable: the two strains have very strong adaptability, can adapt to a water body environment with pH of 4.0-8.0 and salinity of 0.1-3%, and have No illumination aerobic respiration effect under the condition that the Rhodoblast sphagnicola PNSB-MHW CGMCC No.20882, thereby ensuring the wide applicability of the matching use of the two strains. In addition, Rhodoblast sphagnola PNSB-MHW CGMCC No:20882 acid and alkali resistance firstly degrades organic acid micromolecule substances in acid starch wastewater to raise the pH value of the wastewater, and the added Bacillus atrophaeus can secrete a large amount of amylase and other oneThe enzymes degrade macromolecular substances in starch, generate organic acid and micromolecular substances to reduce the pH value of the environment, stimulate the activity of the bacterial strain PNSB-MHW, and the viable count of Rhodoblast sphagnola PNSB-MHW CGMCC No:20882 in the composite microbial agent>1010cfu/mL, number of viable bacteria of Bacillus atrophaeus>109cfu/mL, can automatically adjust the pH value of the water body, and enables the thallus degradation wastewater to be in a stable state all the time.
(3) The new bacterium Rhodoblast sphagnicola coupling composite bacterium agent provided by the invention is applied to degradation of starch wastewater, and the number of viable bacteria of Rhodoblast sphagnicola PNSB-MHW CGMCC No:20882 in the degraded wastewater is more than 108cfu/mL, according to the experiment, the two bacteria have good synergistic coupling, the Bacillus atrophaeus enhances the activity of Rhodoblast sphagnicola PNSB-MHW CGMCC No:20882, and the odor generated by the wastewater, volatile odor organic acid, mercaptan and the like are degraded while pollutants such as COD, ammonia nitrogen, nitrate nitrogen, total phosphorus and the like in the starch wastewater are efficiently degraded, so that the wastewater is deodorized, and data support is provided for the application of the Bacillus atrophaeus in the aspect of biological bacterial manure.
(4) The new bacterium Rhodoblast sphagnola coupling composite bacterium agent provided by the invention is applied to degrading starch wastewater, the degraded starch wastewater has no odor, the self-flocculation phenomenon appears after standing for a certain time, bacterial sludge is settled to the bottom of a container according to the settling rate of 3cm/d, and the turbidity (suspended matters) of supernatant is less than 100 mg/L. The settled bacterial sludge can be recycled, thereby further reducing the cost of wastewater treatment.
(5) The novel bacterium Rhodoblastus sphagnoides coupling composite bacterium agent provided by the invention is applied to degrading starch wastewater, the Rhodoblastus sphagnoides PNSB-MHW CGMCC No.20882 coupling Bacillus atrophaeus (Bacillus atrophaeus) composite bacterium agent provided by the invention can degrade macromolecular substances which are difficult to degrade in starch wastewater, and metabolites can activate other microbial activities in a natural system.
Drawings
FIG. 1 is a phylogenetic tree of Rhodoblast sphagnola PNSB-MHW constructed based on the 16S rDNA gene.
FIG. 2 is a phylogenetic tree of Bacillus atrophaeus (BA-BC) constructed based on the 16S rDNA gene.
FIG. 3 is a graph showing the absorbance peaks of viable Rhodoblast sphagnicola PNSB-MHW bacterial cytochrome.
FIG. 4 is a graph showing the effect of the initial pH of various media on the viable count of Rhodoblast sphagna PNSB-MHW.
FIG. 5 is a graph showing the effect of different salt concentrations on the viable count of Rhodoblast sphagnicola PNSB-MHW.
FIG. 6 is a graph showing the effect of different temperatures on the viable count of Rhodoblast sphagnicola PNSB-MHW.
FIG. 7 is a graph showing the effect of different light intensities on the viable count of Rhodoblast sphagnicola PNSB-MHW.
FIG. 8 is a graph showing the growth curve of Rhodoblast sphagnola PNSB-MHW and the pH change curve of the fermentation broth.
FIG. 9 is a graph showing the growth curve of Bacillus atrophaeus and the pH variation of its fermentation broth.
FIG. 10 is a graph showing the growth curve and pH change curve of the fermentation broth of a Rhodoblast sphagnola PNSB-MHW complex strain.
FIG. 11 is a diagram showing the optimization of the culture conditions of Rhodoblast sphagnola PNSB-MHW coupled Bacillus atrophaeus complex, in which A is the comparison of the COD degradation efficiency of the complex microbial inoculum with different viable count ratios; b, comparing the pH values of the fermentation culture media of the composite microbial inoculum with different viable count ratios; C. comparing the flocculation settling rates of the composite microbial inoculum with different viable count ratios; d, when the viable count ratio of Rhodoblast sphagnola PNSB-MHW to Bacillus atrophaeus is 10:1, the curve of the composite microbial inoculum for COD degradation and the curve of pH change are obtained.
FIG. 12 is a graph showing the effect of different Rhodoblast sphagnola coupled complex inoculum amounts and treatment modes on the COD degradation efficiency of starch wastewater.
FIG. 13 shows the effect of Rhodoblast sphagna coupled complex inoculant on starch wastewater degradation at different sodium acetate addition levels.
FIG. 14 is a graph showing the degradation of starch wastewater by Rhodoblast sphagnicola coupled complex bacterial agents.
Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples. All raw and auxiliary materials selected for use in the present invention, as well as methods for culturing the selected bacterial species, are well known and used in the art, and all percentages referred to herein are by weight unless otherwise indicated.
Rhodoblast sphagnola PNSB-MHW enrichment medium: KH (Perkin Elmer)2PO4 0.04g/L,NH4Cl0.1g/L,MgCl2﹒6H2O 0.01g/L,CaCl2﹒2H2O 0.05g/L,CH3COONa1.0g/L, microelement mother liquor 1ml, vitamin complex mother liquor 1ml, pH 5.0.
Rhodoblast sphagnola PNSB-MHW isolation Medium (RCVBN) (g/L): KH (Perkin Elmer)2PO4 0.5g/L,K2HPO4 0.3g/L，NH4Cl 1.0g/L,MgCl2﹒6H2O 0.2g/L,CaCl2﹒2H2O 0.5g/L,CH3COONa 1.0g/L,CH3CH2COONa 0.5g/L, yeast powder 0.1g/L, agar 7.0g/L, microelement mother liquor 1ml, and pH 5.0.
Example 1: isolation and characterization of Rhodoblast sphagnoides PNSB-MHW and Bacillus atrophaeus (Bacillus atrophaeus) BA-BC
Identification of Rhodoblast sphagnola PNSB-MHW
(1) Rhodoblast sphagnola PNSB-MHW separation and purification
The strain is separated from a water sample of a plum blossom monosodium glutamate acidic wastewater drainage ditch in the five ditches of Xinjiang, the pH of the wastewater is 4.5, and sediment is collected. And carrying out directional enrichment by using an enrichment culture method. Culturing at 25 deg.C for 7 days with illumination intensity of 2000lx, and collecting thallus for strain separation after the culture medium color turns into red. Coating the RCVBN culture medium with the diluted thallus, using the anaerobic culture tank, introducing carbon dioxide to the top layer of the tank body to empty the air, then sealing and placing the tank body in an illumination incubator, culturing at the constant temperature of 25 ℃, and performing colony separation according to the colony appearance time, size and colony morphology on a flat plate, wherein the illumination incubator is used for carrying out illumination culture at the constant temperature of 25 ℃.
(2) Rhodoblast sphagnola PNSB-MHW taxonomic identification
Rhodoblast sphagnola PNSB-MHW (hereinafter referred to as "strain PNSB-MHW") 16Sr DNA sequencing and analysis, total DNA of the strain PNSB-MHW was extracted as a template for PCR amplification using bacterial 16S rDNA universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1429R (5'-CTACGGCTACCTTGTTACGA-3'). The PCR reaction system was 25. mu.L: DNA template 1.0. mu.L, 10. mu. mol/L27F/1429R each 1.0. mu.L, 10mmol/L dNTPs 1.5. mu.L, 10 XPCR Buffer (2.5mmol/L MgCl)2) 2.5. mu.L, 2.5U/. mu.L TaqDNA polymerase 0.5. mu.L, sterile ultrapure water was made up to 25. mu.L. The reaction conditions are as follows: pre-denaturation at 94 ℃ for 5 min; denaturation at 94 ℃ for 45s, annealing at 58 ℃ for 45s, extension at 72 ℃ for 1.5min, and 35 cycles; extension at 72 deg.C for 7min, and storage at 4 deg.C. After the amplification product is detected by 10g/L agarose gel electrophoresis, the PCR product is subjected to clone sequencing by Shanghai biological engineering Co., Ltd, and the sequencing result is shown as SEQ ID No. 1.
The obtained sequence results were subjected to BLAST search at the National Center for Biotechnology Information (NCBI) of the United states, a model strain having high similarity was selected as a reference strain, and a 16S rDNA gene phylogenetic tree was constructed using the Neighbor-joining method (Neighbor-joining) in MEGA5.0 software, with a self-developed value (Bootstrap) of 1000. As a result, as shown in FIG. 1, the strain PNSB-MHW forms a branch with a plurality of strains of Rhodoblast sphagnola, the 16S rDNA gene sequence has high similarity with a model strain of Rhodoblast sphagnola (AM040096), wherein the homology with the Rhodoblast sphagnola (AM040096) strain is the highest and reaches 98.32%, and the identification of the strain at the molecular level of the known strain system and the verification of the physiological and biochemical system tests of the combined strain through the comprehensive judgment of the similarity and the homology of the strain confirm that the obtained strain number in the acidophilic photosynthetic bacteria genus is PNSB-MHW as a typical new strain.
Based on the above biological characteristics, the strain PNSB-MHW was identified as Rhodoblast sphagna. The strain has been deposited in the Budapest treaty International Collection of microorganisms: china general microbiological culture Collection center (CGMCC for short). And (4) storage address: the institute of microbiology, national academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, Beijing. The preservation date is 10 months and 13 days in 2020, and the preservation number is CGMCC NO. 20882.
(II) separation and identification of Bacillus atrophaeus BA-BC
Bacillus atrophaeus (Bacillus atrophaeus) BA-BC was isolated from soil in a certain salt field of Tuzibetuz, Behcet county, Aksu, Xinjiang. Collecting saline soil with the thickness of 0-10cm on the surface layer of a salt field, using an LB culture medium, adding 5% sodium chloride, uniformly coating diluent with different concentrations on an LB solid plate containing salt by adopting a dilution gradient method, placing the LB solid plate containing salt at the constant temperature of 30 ℃ for culturing for 24h, selecting single colonies according to different colony forming, streaking and purifying, then carrying out 16SrRNA gene sequencing, and constructing a 16S rDNA gene phylogenetic tree by utilizing an adjacency method (Neighbor-join) in MEGA5.0 software, wherein the result is shown in figure 2. The result shows that the strain has 99.45% similarity with Bacillus atrophaeus (Bacillus atrophaeus), and the phylogenetic tree analysis result also shows that the strain belongs to Bacillus atrophaeus cluster, and the Bacillus atrophaeus (Bacillus atrophaeus) BA-BC belongs to common Bacillus atrophaeus (Bacillus atrophaeus).
Example 2: biochemical testing of Rhodoblast sphagnola PNSB-MHW
(1) Bacterial strain PNSB-MHW viable bacteria cytochrome light absorption wavelength
The result of the light absorption wavelength of the viable bacteria cytochrome of the strain PNSB-MHW is shown in figure 3, wherein 4 characteristic peaks appear in the range of 500-900nm and are respectively located at 480nm, 510nm, 590nm and 810nm, and belong to the positions of the light absorption peaks of chlorophyll a of typical purple non-sulfur photosynthetic bacteria, but are clearly different from the strains in the common genus range of acidophilic photosynthetic bacteria and belong to the characteristics of new strains in the genus of acidophilic photosynthetic bacteria.
(2) Growth of the Strain PNSB-MHW under different initial pH conditions
The growth conditions of the bacterial strain PNSB-MHW under different initial pH conditions are shown in figure 4, the optimal growth pH of the bacterial strain is 5.0-5.5, the growth of the bacterial strain is reduced along with the increase of the pH, the activity of the bacterial strain is completely inhibited when the pH is 3.5 and 9.0, the bacterial strain can grow when the pH is 4.0 and 8.5, the activity is only 30% of the highest activity, and the activity of the bacterial strain is 60% when the pH is 7.5, so that the bacterial strain belongs to acidophilic bacteria, generally likes to be in an acidic environment, but has certain tolerance to alkali, but has a clear difference with the common strains in the same genus of acidophilic photosynthetic bacteria, and belongs to the characteristics of new strains in the same genus of acidophilic photosynthetic bacteria.
(3) Growth of the strain PNSB-MHW under different salt concentration conditions
The growth condition of the bacterial strain PNSB-MHW under the condition of different salt concentrations is shown in figure 5, the viable count of the bacterial strain is higher when the concentration of sodium chloride is 0.5-3%, the growth activity of the bacterial strain is highest under the condition of 1-1.5% salt concentration, the growth of the bacterial strain is obviously influenced when the concentration of salt is higher than 3.5%, the activity is reduced by 70%, the activity is basically and completely inhibited when the concentration of salt is higher than 4%, the bacterial strain does not grow, but has a distinct salt tolerance difference with the bacterial strain in the common acidophilic photosynthetic bacteria same genus range, and belongs to the characteristics of a new bacterial strain in the acidophilic photosynthetic bacteria same genus.
(4) Growth conditions of the strain PNSB-MHW under different culture temperature conditions
The growth conditions of the bacterial strain PNSB-MHW under different culture temperature conditions are shown in figure 6, and the result shows that the optimal growth temperature of the bacterial strain is 30 ℃, the activity of the bacterial strain is higher at 20-25 ℃, the activity growth of the bacterial strain is inhibited when the temperature exceeds 40 ℃, and the thallus basically does not grow when the temperature is 45 ℃, but has distinct growth characteristics compared with the strains in the common acidophilic photosynthetic bacteria congeneric range and belongs to the characteristics of new strains in the acidophilic photosynthetic bacteria congeneric range.
(5) Growth conditions of the strain PNSB-MHW under different illumination intensities
The influence of the illumination intensity on the growth of the strain PNSB-MHW is shown in figure 7, and the result shows that the strain has higher growth activity under the light intensity of 500 to 6000lx, wherein the strain has the highest growth performance under the illumination intensity of 1000-. When the illumination intensity exceeds 10000lx, the activity of the strain is inhibited, which shows that the cytochrome reaches light saturation and the growth of the strain is inhibited, but the strain has a distinct difference on the illumination intensity compared with the common strains in the same genus of acidophilic photosynthetic bacteria and belongs to the characteristics of new strains in the same genus of acidophilic photosynthetic bacteria.
Example 3: preparation of Rhodoblast sphagnola coupled complex microbial inoculum
The present embodiment further provides a preparation method of a rhodoblast sphagna coupling complex bacterial agent based on the embodiments 1 to 2, and the preparation method specifically includes the following steps:
(1) inoculating the preserved strain Rhodoblast sphagnicola PNSB-MHW CGMCC No:20882 in a liquid modified Nisson and Dundas culture medium, standing and culturing in an illumination culture box with illumination intensity of 1000-; inoculating Bacillus atrophaeus into liquid self-grinding culture medium, and culturing in a shaker at 30 deg.C and rotation speed of 120rad/min for 3-5d to stationary phase, wherein the thallus concentration contains 15g dry bacteria per liter.
(2) And (2) taking the two bacterial liquids cultured in the step (1), respectively carrying out centrifugal separation at room temperature, discarding the supernatant, precipitating, fully suspending with sterile water, centrifuging again, repeating the step for 3-5 times, and suspending the precipitate with sterile water to obtain two bacterial strain liquid seeds.
(3) And (3) respectively compounding the Rhodoblastus sphagnella PNSB-MHW obtained in the step (2) and liquid seeds of Bacillus atrophaeus according to a volume ratio under an aseptic condition to prepare the Rhodoblastus sphagnella coupling compound microbial inoculum.
Example 4: preparation of Rhodoblast sphagnola coupled complex microbial inoculum
In this example, on the basis of example 3, the culture conditions of the strain PNSB-MHW, the culture conditions of Bacillus atrophaeus and the coupling culture conditions of the two were examined.
(1) Culture of Rhodoblast sphagnola PNSB-MHW
The strain PNSB-MHW is statically cultured on a modified Nisson & Dundas culture medium in a light culture box with the light intensity of 1000-.
As can be seen from the data in FIG. 3, Rhodoblast sphagnicola PNSB-MHW starts to enter the growth exponential phase from the 2 nd day of initial inoculation, reaches the plateau phase from the 8 th day, and the viable count of the thalli is not obviously reduced after the thalli is kept for 12 days, and is 6.86x10 after the culture is finished10cfu/ml. In addition, the pH value of the fermentation medium shows that Rhodoblast sphagnola PNSB-MHW can survive under the condition that the initial pH is 4.0, and the pH value is between 8.0 and 9.0 after the fermentation is finished, which shows that the Rhodoblast sphagnola PNSB-MHW can have wide acid-base adaptability and can produce alkali during the growth process, but the Rhodoblast sphagnola PNSB-MHW has the characteristics of being different from the characteristics of producing alkali in the common acidophilic photosynthetic bacteria congener, and belongs to a new strain in the acidophilic photosynthetic bacteria congener.
(2) Culture of Bacillus atrophaeus
Culturing the separated and purified Bacillus atrophaeus (Bacillus atrophaeus) on a self-grinding culture medium in a shaking table with the rotation speed of 1200rpm at the temperature of 30 ℃ for 3-5d until the stationary phase. The growth condition of Bacillus atrophaeus (Bacillus atrophaeus) and the pH change of the fermentation liquor are examined, and the specific result is shown in figure 4.
As can be seen from the data in FIG. 4, Bacillus atrophaeus (Bacillus atrophaeus) can grow a large number of viable bacteria from the 2 nd day, and enter the exponential phase, and enter the logarithmic phase from the 5 th day, and the viable bacteria number of Bacillus atrophaeus does not decrease within 15 days along with the increase of the storage time, and the viable bacteria number is 9.11 × 10 after the culture is finished12cfu/ml. Bacillus atrophaeus (Bacillus atrophaeus) can utilize acid-producing substances such as starch and glucose contained in a culture medium to reduce the pH value of a fermentation liquor, the pH value is reduced along with the increase of the culture time, and the pH value is stabilized at the level after the pH value is reduced to about 4.0 at the 5 th day. Therefore, Bacillus atrophaeus (Bacillus atrophaeus) can have tolerance to low pH environments, which can produce acids using sugars.
(3) Preparation of Rhodoblast sphagnola coupled complex microbial inoculum
On the basis of the test 1-2, the test uses a Bacillus atrophaeus culture medium, the pH of which is lowered to 4.5 by adding a sterilized lactic acid bacteria fermentation broth, and Rhodoblastus sphagnicola PNSB-MHW and Bacillus atrophaeus are inoculated into the medium in a volume ratio of 1: 1. And the number of live bacteria of each thallus and the pH change of the fermentation liquor are measured by using the bacterial strain PNSB-MHW which is only inoculated and the bacillus atrophaeus which is only inoculated as the control groups, and the specific results are shown in the attached figure 10.
As can be seen from the data in FIG. 10, Bacillus atrophaeus (Bacillus atrophaeus) did not grow at an initial pH of 4.5, and the cell concentration was almost unchanged, whereas the cell concentration of Bacillus atrophaeus PNSB-MHW was increased after the Rhodoblast sphagna strain PNSB-MHW was prepared, and the OD of 16d cultured cells was 2 times that of 16d cultured cells alone. The photosynthetic bacteria show the same change curve, the OD value of the Rhodoblastus sphagnoides PNSB-MHW bacteria is increased along with the increase of the culture time, the Rhodoblastus sphagnoides PNSB-MHW bacteria concentration shows a straight-line rising trend after the Bacillus atrophaeus is compounded, and the viable count of the Rhodoblastus sphagnoides PNSB-MHW bacteria is about 4 times of that of the Rhodoblastus sphagnoides PNSB-MHW bacteria cultured independently. Meanwhile, monitoring the dynamic change of the pH of the culture medium is carried out, the pH of the bacillus atrophaeus PNSB-MHW is not changed and is maintained in an initial state, the pH of the Rhodoblastus sphagnicola PNSB-MHW is increased along with the increase of the culture time and finally is increased to about 7.5, the pH of the coupled complex bacteria is increased along with the increase of the culture time in the first 8 days, but is sharply decreased in the 9 th day, is decreased to the lowest 5.0 in the last 11 days, then is linearly increased, and finally is about 9.0. It is shown that the combination of the two bacteria can act to mutually promote the growth of each other, mainly by releasing the repressor that affects the growth of both parties. Rhodoblastus sphagnoides PNSB-MHW can utilize organic acid produced by decomposing sugar starch by using spore bacteria to generate alkaline substances, so that the pH repression of the spore bacteria is relieved, the spore bacteria can be promoted to continue to grow after the repression is relieved, organic matters are decomposed to continue to produce acid, the deficiency of nutrient substances for the growth of photosynthetic bacteria is relieved, the growth of photosynthetic bacteria is stimulated to produce alkali, the pH is adjusted, the growth of Bacillus sphagnoides is inhibited due to limited substrates, and the growth of Rhodoblastus sphagnoides PNSB-MHW bacteria is also inhibited. After fermentation is finished, the pH value of the culture medium is about 8.5, the number of live bacteria of the two bacteria is several times higher than that of live bacteria of the two bacteria which are cultured independently, and the self-flocculation precipitation phenomenon occurs in both Bacillus atrophaeus (Bacillus atrophaeus) and Rhodoblast sphagna PNSB-MHW.
Example 5: optimized culture of Rhodoblast sphagnella PNSB-MHW CGMCC No:20882 coupled bacteria composite bacterial agent
In order to verify the actual degradation effect of the Rhodoblastus sphagnoides PNSB-MHW CGMCC No:20882 coupled composite microbial inoculum on starch wastewater, the starch wastewater is used as a culture medium, the influence of the Rhodoblastus sphagnoides PNSB-MHW CGMCC No:20882 and Bacillus atrophaeus on the COD degradation rate and pH of the starch wastewater by the optimal compounding ratio, the inoculation amount and the culture mode (light anaerobic, dark aerobic, light anaerobic-dark aerobic) is explored, and the flocculation condition of the degraded wastewater is compared.
(1) Effect of different Rhodoblast sphagna PNSB-MHW and Bacillus atrophaeus composite ratios on starch wastewater degradation efficiency
The other conditions are not changed, and the test examines the influence of the compounding ratio of different Rhodoblastus sphagnoides PNSB-MHW CGMCC No:20882 and Bacillus atrophaeus on the degradation efficiency of the starch wastewater. As shown in the accompanying FIG. 11, it can be seen from the data of FIG. 11 that, when the ratio of Rhodoblast sphagna PNSB-MHW (PSB) to Bacillus Atrophaeus (BA) is 10:1 by volume, the highest COD degradation efficiency of starch wastewater is 98%, the pH value at the end of fermentation is about 8.0, and the pH change during fermentation is low and can be maintained between 7 and 8; the flocculation status of the wastewater after degradation was further determined to be 5cm/d when the volume ratio of Rhodoblast sphagnola PNSB-MHW (PSB) to Bacillus Atrophaeus (BA) was 10: 1.
(2) Test of inoculation amount on COD (chemical oxygen demand) degradation efficiency of starch wastewater
On the basis of the determination of the viable count ratio in the test (1), the influence of the inoculation amount and the inoculation mode on the degradation efficiency of the starch wastewater is examined, and the result is shown in the attached figure 12. As can be seen from the data in the attached figure 12, the COD degradation efficiency shows a rising trend along with the increase of the inoculation amount, wherein the starch wastewater degradation rate exceeds 90% when the inoculation amount exceeds 15%, and the inoculation amount is 15% based on the practical application of cost consideration; further comparisons of treatment regimes were made at 15% inoculation conditions, and the results are shown in figure 12. The highest COD degradation efficiency is 95% under the illumination anaerobic-dark aerobic condition, and then the anaerobic treatment is carried out in the dark. Because photosynthetic bacteria can rapidly grow and degrade COD and produce alkali under the anaerobic illumination condition, and bacillus atrophaeus belongs to aerobic bacteria and needs to function under the aerobic condition, the set illumination anaerobic-dark aerobic condition is that firstly illumination anaerobic is carried out for 48h, Rhodoblast sphagnola PNSB-MHW grows and produces alkali, and then anaerobic illumination: the reaction was carried out in a dark atmosphere of 12h to 12h, under which the COD degradation efficiency was 95.21%.
(3) Test of Effect of intermediate metabolites on starch degradation Rate
Since acetic acid is an intermediate metabolite of many anaerobic reactions, it is also an important substrate for stimulating the growth of photosynthetic bacteria. Therefore, the effect of adding a certain amount of sodium acetate into the starch wastewater on the COD degradation efficiency is further examined, and the result is shown in figure 13. Compared with a control group, the degradation rate of the COD in the starch wastewater can be improved by adding sodium acetate with different concentrations, but the improvement range is not large, and the degradation rate of the COD in the starch wastewater can be improved to about 98% by adding 1g/L of sodium acetate according to the addition concentration.
Example 6: application of Rhodoblast sphagnola coupling compound bacterial agent in starch wastewater degradation
On the basis of examples 2-3, the optimal compounding ratio of the Rhodoblastus sphagnoides PNSB-MHW and Bacillus atrophaeus (Bacillus atrophaeus) in the Rhodoblastus sphagnoides coupled composite microbial inoculum of the invention is verified, the influence of the Rhodoblastus sphagnoides PNSB-MHW coupled composite microbial inoculum on indexes such as ammonia nitrogen, nitrate nitrogen, total phosphorus, mercaptan, hydrogen sulfide and the like in starch wastewater along with the increase of degradation time is examined, and the specific result is shown in the attached figure 14.
As shown in the data in the attached figure 14, the degradation rates of Rhodoblast sphagnola PNSB-MHW coupling complex bacteria on ammonia nitrogen, nitrate nitrogen and total phosphorus in water body are all 6d under the optimal condition>99 percent and has mercaptan and hydrogen sulfide degradation effect on starch wastewater malodor sourceThe rate also reaches more than 98 percent, and the water quality reaches the national sewage discharge standard. After the wastewater treatment is finished, the number of two coupled composite bacteria in the wastewater is measured, and the number of viable bacteria of Rhodoblast sphagna PNSB-MHW can reach 1.19 multiplied by 108cfu/ml, the viable count of Bacillus atrophaeus can reach 6.21 multiplied by 108cfu/ml. Rhodoblast sphagna PNSB-MHW and Bacillus atrophaeus are compounded according to the volume ratio of 10:1, the inoculation amount is 15% of the total volume of the starch wastewater, anaerobic illumination (1000-: and (2) continuously culturing for 72h in 12h in a dark and aerobic manner, wherein 1g/L of sodium acetate is additionally added into the sewage before the culture, the COD (chemical oxygen demand) degradation rate of the starch wastewater can reach 98.21%, the degradation efficiency of pollutants such as ammonia nitrogen, nitrate nitrogen, total phosphorus, mercaptan, hydrogen sulfide and the like in a water body is over 98%, and the pH of a fermentation liquor is about 8.0 after the fermentation is finished, under the pH condition, thalli start to self-flocculate and can precipitate suspended particles and high-chroma granular substances (8cm/h) in the sewage, so that the wastewater can be discharged without additional treatment and can be used as greening raw water for irrigation or washing and recycling water.
The above examples are merely illustrative for clearly illustrating the present invention and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made while remaining within the scope of the present invention.
Sequence listing
<110> institute of microorganism application of Sinkiang academy of agricultural sciences (Xinjiang-Yameiya bioengineering research and development center, China)
<120> Rhodoblast sphagna coupling complex bacterial agent and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1418
<212> DNA
<213> Rhodoblastus sphagnicola(Rhodoblastus sphagnicola)
<400> 1
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ggaatggccc gcgtccgatt agctagttgg tgaggtaacg gctcaccaag gcgacgatcg 240
gtagctggtc tgagaggatg atcagccaca ttgggactga gacacggccc aaactcctac 300
gggaggcagc agtggggaat attggacaat gggcgcaagc ctgatccagc catgccgcgt 360
gagtgatgac ggccttaggg ttgtaaagct ctttcaccca cgacgataat gacggtagtg 420
ggagaagaag ccccggctaa cttcgtgcca gcagccgcgg taatacgaag ggggctagcg 480
ttgttcggat ttactgggcg taaagcgcac gtaggcggat ctttaagtca ggggtgaaat 540
gccggagctc aacttcggaa ctgccttaga tactggagat ctagagatcg agagacgtga 600
gtggaactgc gagtgtagac gtgaaattcg tagatattcc caagaacacc agtggcgaag 660
gcggctcaca ggctcgatac tgacgctgag gtgcgaaagc gtggggagca aacaggatta 720
gataccctgg tagtccacgc cgtaaacgat ggatgctagc cgttaggcag cttgctgctt 780
agtggcgcag ctaacgcttt aagcatcccg cctggggagt acggtcgcaa gattaaaact 840
caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtataatt cgatgcaacg 900
ggcagaacct taccagcttt tgacatgtcc tggacggttt ccagagatgg attcctctct 960
tcggagccag gaacacaggt gctgcatggc tgtcgtcagc tcgtgtcgtg agatgttggg 1020
ttaagtcccg caacgagcgc aacccacgtc cttagttgcc aacatttagt tgggcactct 1080
agggagactg ccggtgataa ggggagagga aggtgtggat gatgtcaagt gctcatggcc 1140
cttacacgct cggctacaca cgtgctacaa tggcggtgac aatgggatgc gaaggggcga 1200
cccttagcaa atctcaaaaa tccgtctcag ttcggattgt tctctgcaac tcgagagcat 1260
gaaggtggaa tcgctagtaa tcgcagatca gaacgctgcg gtgaatacgt tcccgggcct 1320
tgtacacacc gcccgtcaca ccatgggagt tggctttacc cgaaggcgtt tcgctaaccg 1380
caaggaggca gacgaccacg gtagggtcag cgactggg 1418
Claims (10)
1. The Rhodoblastus sphagnoides coupling complex microbial inoculum is characterized by being prepared by compounding Rhodoblastus sphagnoides PNSB-MHW and Bacillus atrophaeus.
2. The Rhodoblastus sphagnicola coupled complex inoculant according to claim 1, wherein the coupled complex inoculant consists of Rhodoblastus sphagnicola PNSB-MHW compounded with Bacillus atrophaeus (Bacillus atrophaeus) in a volume ratio of 10: 1.
3. The Rhodoblastus sphagnoides coupled complex bacterial agent as claimed in claim 1, wherein the viable count of Rhodoblastus sphagnoides PNSB-MHW and Bacillus atrophaeus (Bacillus atrophaeus) in the coupled complex bacterial agent is 10 or more9cfu/mL。
4. The Rhodoblastus sphagnicola coupled complex inoculant according to claim 1, wherein the Rhodoblastus sphagnicola PNSB-MHW is deposited at the China Committee for culture Collection of microorganisms under accession number: CGMCC No.20882, preservation date: 10 and 13 days in 2020.
5. The Rhodoblastus sphagnicola coupled complex inoculant according to claim 1, wherein the gene sequence of Rhodoblastus sphagnicola PNSB-MHW is shown in SEQ ID NO 1.
6. The Rhodoblastus sphagnicola coupled complex inoculant as claimed in claim 1, wherein the Rhodoblastus sphagnicola PNSB-MHW rich medium: KH (Perkin Elmer)2PO4 0.04g/L,NH4Cl 0.1g/L,MgCl2﹒6H2O 0.01g/L,CaCl2﹒2H2O 0.05g/L,CH3COONa1.0g/L, microelement mother liquor 1ml, vitamin complex mother liquor 1ml, pH 5.0.
7. The Rhodoblastus sphagnoides coupled complex inoculant according to claim 1, wherein Rhodoblastus sphagnoides PNSB-MHW separation medium (RCVBN) (g/L): KH (Perkin Elmer)2PO40.5g/L,K2HPO40.3g/L，NH4Cl 1.0g/L,MgCl2﹒6H2O 0.2g/L,CaCl2﹒2H2O 0.5g/L,CH3COONa 1.0g/L,CH3CH2COONa 0.5g/L, yeast powder 0.1g/L, agar 7.0g/L, microelement mother liquor 1ml, and pH 5.0.
8. A preparation method of a Rhodoblast sphagnola coupling complex microbial inoculum is characterized by comprising the following steps:
(1) inoculating the preserved strain Rhodoblast sphagnicola PNSB-MHW CGMCC No:20882 in a liquid modified Nisson and Dundas culture medium, standing and culturing in an illumination culture box with illumination intensity of 1000-; inoculating selected Bacillus atrophaeus (Bacillus atrophaeus) in a liquid self-grinding culture medium, and culturing in a shaking table at 30 deg.C and rotation speed of 120rad/min for 3-5d to a stabilization period, wherein the thallus concentration contains 15g dry bacteria per liter;
(2) taking the two bacterial liquids cultured in the step (1), respectively carrying out centrifugal separation at room temperature, discarding supernatant, precipitating, fully suspending with sterile water, centrifuging again, repeating the step for 3-5 times, and suspending the precipitate with sterile water to obtain two bacterial strain liquid seeds;
(3) and (3) respectively compounding the Rhodoblastus sphagnella PNSB-MHW obtained in the step (2) and liquid seeds of Bacillus atrophaeus according to a volume ratio under an aseptic condition to prepare the Rhodoblastus sphagnella coupling compound microbial inoculum.
9. The use of the Rhodoblast sphagnicola coupled complex inoculant according to claim 1 in the degradation of starch wastewater.
10. The application of the Rhodoblastus sphagnicola coupled complex microbial inoculum in degrading starch wastewater as claimed in claim 9, wherein the Rhodoblastus sphagnicola coupled complex microbial inoculum is inoculated in starch wastewater according to the inoculation amount of 15% by mass and volume, the inoculation amount is 30 ℃, after 72 hours of light anaerobic culture, anaerobic light-dark aerobic alternate culture is carried out for 72 hours, and the wastewater degradation process is finished after the bacteria are completely self-flocculated and precipitated after 24 hours of standing.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289933A (en) * | 2013-06-06 | 2013-09-11 | 扬州大学 | Bacillus amyloliquefaciens plant subspecies B-01 and application thereof |
| CA2909780A1 (en) * | 2013-04-17 | 2014-10-23 | Envera, Llc | Bacillus strains and compositions |
| CN105567595A (en) * | 2016-01-08 | 2016-05-11 | 陕西博秦生物工程有限公司 | Bacillus atrophaeus 5-2a and applications thereof |
| WO2017005601A1 (en) * | 2015-07-09 | 2017-01-12 | Chr. Hansen A/S | Fermented milk inoculated with both lactic acid bacteria (lab) and bacillus |
| CN110283740A (en) * | 2019-05-31 | 2019-09-27 | 新疆天康饲料科技有限公司生物添加剂分公司 | A kind of degradation high-ammonia-nitrogen sewage composite bacteria agent and its application in processing sewage |
| CN111454861A (en) * | 2020-04-10 | 2020-07-28 | 北京盛合正泰环保工程有限公司 | Bacillus amyloliquefaciens for efficiently purifying sewage, microbial agent and application |
-
2020
- 2020-11-17 CN CN202011291526.1A patent/CN112481153B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2909780A1 (en) * | 2013-04-17 | 2014-10-23 | Envera, Llc | Bacillus strains and compositions |
| CN103289933A (en) * | 2013-06-06 | 2013-09-11 | 扬州大学 | Bacillus amyloliquefaciens plant subspecies B-01 and application thereof |
| WO2017005601A1 (en) * | 2015-07-09 | 2017-01-12 | Chr. Hansen A/S | Fermented milk inoculated with both lactic acid bacteria (lab) and bacillus |
| CN105567595A (en) * | 2016-01-08 | 2016-05-11 | 陕西博秦生物工程有限公司 | Bacillus atrophaeus 5-2a and applications thereof |
| CN110283740A (en) * | 2019-05-31 | 2019-09-27 | 新疆天康饲料科技有限公司生物添加剂分公司 | A kind of degradation high-ammonia-nitrogen sewage composite bacteria agent and its application in processing sewage |
| CN111454861A (en) * | 2020-04-10 | 2020-07-28 | 北京盛合正泰环保工程有限公司 | Bacillus amyloliquefaciens for efficiently purifying sewage, microbial agent and application |
Non-Patent Citations (3)
| Title |
|---|
| IRINA S. KULICHEVSKAYA ET AL.: "Rhodoblastus sphagnicola sp. nov., a novel acidophilic purple non-sulfur bacterium from Sphagnum peat bog", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 56, pages 1397 * |
| 张新刚 等: "光合细菌处理污水过程中降解淀粉微生物生长情况及污水降解效果的研究", 安徽农业科学, vol. 41, no. 22, pages 9407 - 9408 * |
| 王剑秋 等: "光合细菌法降解淀粉废水积累菌体蛋白的研究", 清华大学学报(自然科学版), vol. 47, no. 3, pages 348 - 351 * |
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