CN112481100A

CN112481100A - Mesenchymal stem cell culture medium production system

Info

Publication number: CN112481100A
Application number: CN202110000365.4A
Authority: CN
Inventors: 何鸿寅
Original assignee: Xi'an Lanhua Feiteng Biotechnology Co ltd
Current assignee: Xi'an Lanhua Feiteng Biotechnology Co ltd
Priority date: 2021-01-03
Filing date: 2021-01-03
Publication date: 2021-03-12

Abstract

The invention discloses a mesenchymal stem cell culture medium production system in the technical field of mesenchymal stem cell culture medium production, which comprises a blending part, wherein a quantitative liquid injection part is assembled below the blending part, and a belt conveying part is arranged below the quantitative liquid injection part; the invention solves the problem that when people control a discharge valve of a mixing drum of a culture solution adding device to discharge, the volume of the culture solution added into each culture medium is difficult to accurately grasp, so that the adding amount of the nutrient solution in each culture medium is inconsistent, and meanwhile, in the process of resetting the first scraper plate, the first scraper plate can scrape off large granular substances at the top of the filter tank, so that the problems that after a filter plate of the culture solution adding device in the prior art filters the culture solution, the large granular substances can be remained at the top and block a filter opening, so that the culture solution entering a culture dish mechanism is reduced in the same time, so that the amount of the culture solution added into the culture dish mechanism at each time is different, and the culture effect of the culture dish mechanism is different are solved.

Description

Mesenchymal stem cell culture medium production system

Technical Field

The invention relates to the technical field of mesenchymal stem cell culture medium production, in particular to a mesenchymal stem cell culture medium production system.

Background

The mesenchymal stem cell belongs to one of human body multipotential stem cells, has the characteristics of multidirectional differentiation potential, hematopoietic support, promotion of stem cell implantation, immune regulation, self-replication and the like, and needs to use a special culture medium for culturing the mesenchymal stem cell.

In the case of mesenchymal stem cell culture medium production disclosed in the prior art, chinese patent with patent application number CN201821634825.9 discloses a mesenchymal stem cell culture medium production device, which comprises a frame, a first feed inlet and a first motor, wherein a water inlet pipe is installed on one side of the upper part of the frame, a first water outlet pipe is arranged below the water inlet pipe, a second water outlet pipe is arranged on one side of the first water outlet pipe, the first feed inlet is installed on the other side of the upper part of the frame, the first motor is fixed above one side of the inner part of the frame, a belt is connected above the first motor, a second motor is installed above a second stirring shaft, a second connecting pipe is arranged on one side of the lower part of the second mixing cavity, and a partition plate is arranged below the second connecting pipe.

After the culture solution that mixes is filtered with culture solution interpolation device to the stirring with the prior art, need pack the culture solution into the culture medium, present main means utilizes personnel control culture solution interpolation device's mixing drum bleeder valve to carry out the ejection of compact, it is difficult to accurately hold the volume of the culture solution in adding the culture medium at every turn, lead to the nutrient solution addition inconsistent in every culture medium, and the filter of present culture solution interpolation device is after filtering the culture solution, the top can persist the large granule material, can block up the filter orifice, lead to the culture solution at same time, the culture solution that gets into in the culture medium reduces, also can make the culture solution in the culture medium reduce, lead to the volume of adding the culture solution at every turn to appear the deviation, influence culture effect.

Based on this, the invention designs a mesenchymal stem cell culture medium production system to solve the above problems.

Disclosure of Invention

The invention aims to provide a mesenchymal stem cell culture medium production system, which aims to solve the problems that after a culture solution which is stirred and mixed is filtered by a culture solution adding device in the prior art, the culture solution needs to be filled into the culture medium, the current main means is to control a discharge valve of a mixing barrel of a culture solution adding device by a person to discharge, the volume of the culture solution added into the culture medium each time is difficult to accurately control, the adding amount of the nutrient solution in each culture medium is inconsistent, and the filter of present culture solution interpolation device can persist the large granule material at the top after filtering the culture solution, can block up the filter orifice, leads to the culture solution in same time, and the culture solution that gets into in the culture medium reduces, also can make the culture solution in the culture medium reduce, leads to the volume of adding the culture solution at every turn to appear the deviation, influences the problem of cultivateing the effect.

In order to achieve the purpose, the invention provides the following technical scheme: a mesenchymal stem cell culture medium production system comprises a blending part, wherein a quantitative liquid injection part is assembled below the blending part, a belt conveying part is arranged below the quantitative liquid injection part, a plurality of culture dish mechanisms are arranged on the belt conveying part in an array manner, and two side faces of each culture dish mechanism are provided with inclined plane top blocks for controlling the quantitative liquid injection part to automatically and quantitatively inject materials;

the quantitative liquid injection part comprises a filter tank, sliding openings are arranged on two sides of the bottom surface of the filter tank, two sliding openings are assembled with a first scraper plate in a sliding mode, two ends of the first scraper plate are fixedly connected with first sliding sleeves, each first sliding sleeve is internally vertically assembled with a sliding ejector rod for butting with an inclined plane ejector block in a sliding mode, the top of each first sliding sleeve is provided with a first reset mechanism for resetting the sliding ejector rod, the tops of the opposite side surfaces of the two sliding ejector rods are respectively provided with a limiting groove, a limiting plate is clamped and limited in the limiting grooves, the positioning plates are respectively assembled with a first guide rail fixed on the side surfaces of the filter tank in a sliding mode, the tops of the first guide rails are respectively fixed with a mounting plate, the mounting plates are respectively and fixedly connected with a third spring for pushing the positioning plates, and are also provided with a second reset mechanism for pulling the first scraper plate to reset, an automatic control mechanism which is matched with the first scraper to move and automatically start the material injection is also arranged in the filter tank;

in the prior art, after a culture solution which is stirred and mixed is filtered by a culture solution adding device, the culture solution needs to be filled into a culture medium, the current main means is that a person controls a discharge valve of a mixing barrel of the culture solution adding device to discharge, the volume of the culture solution which is added into the culture medium each time is difficult to accurately control, the addition amount of the nutrient solution in each culture medium is inconsistent, large granular substances can be remained at the top of a filter plate of the current culture solution adding device after the culture solution is filtered, a filter opening can be blocked, the culture solution which enters the culture medium is reduced in the same time, the culture solution in the culture medium is reduced, and the volume of the culture solution which is added each time is deviated, when the culture medium adding device works, firstly, a uniformly mixing part can uniformly mix the culture solution which needs to be injected into a culture dish mechanism, and uniformly mix raw materials as much as possible, when the belt conveying part conveys the culture dish mechanism and the inclined plane top block on the belt conveying part to the position below the quantitative liquid injection part, the inclined plane top block can act with the sliding ejector rod to enable the sliding ejector rod to obtain the trend of moving upwards and moving rightwards, at the moment, the third spring can push the positioning plate and the limiting groove to be clamped together so as to limit the sliding ejector rod to move upwards, the sliding ejector rod can only move rightwards under the pushing of the inclined plane top block, when the sliding ejector rod moves rightwards, the first scraper can move along with the sliding ejector rod to enable the automatic control mechanism to regulate the quantitative liquid injection part to start to inject materials, when the sliding ejector rod and the positioning plate move to the end part of the first guide rail, the position of the positioning plate is limited by the first guide rail, when the displacement does not occur, the inclined plane ejector block still moves rightwards continuously, after a period of time, the positioning plate can be separated from the limiting groove on the sliding ejector rod, the positioning plate moves upwards under the action of the inclined plane ejector block and is gradually separated from the inclined plane ejector block, after the positioning plate is separated from the inclined plane ejector block, the positioning plate is restored to the initial position under the action of the first reset mechanism, the limiting groove on the positioning plate is clamped with the positioning plate again under the action of a third spring, under the action of the second reset mechanism, the first scraper plate, the first sliding sleeve, the sliding ejector rod and the positioning plate are restored to the initial position, and meanwhile, the automatic control mechanism is controlled to finish the stop material injection of the quantitative liquid injection part, the process is consistent in the time of material injection into the culture dish mechanism every time, so that the automatic material injection of each culture dish mechanism is quantitative, and when personnel control a discharge valve of a mixing barrel of the culture liquid adding device to discharge, be difficult to the volume of accurate assurance culture solution in at every turn adding the culture medium, lead to the inconsistent problem of nutrient solution addition in every culture medium, and simultaneously, at the in-process that first scraper blade resets, first scraper blade can strike off the large particulate matter at filter-tank top, the filter of having solved among the prior art culture solution interpolation device is behind the filtration culture solution, large particulate matter can be stayed at the top, can block up the filtration mouth, lead to the culture solution in same time, the culture solution that gets into in the culture dish mechanism reduces, the volume that leads to adding the culture solution in the culture dish mechanism at every turn is different, the problem that the culture effect of messenger's culture dish mechanism is different.

As a further scheme of the invention, the blending part comprises a shell, an L-shaped fixing plate is fixedly connected on the inner side wall of the shell, a first mixing cylinder is fixedly connected at the top of the L-shaped fixing plate, a first stirring rod is rotatably connected at the top of the first mixing cylinder, a first feeding pipe is fixedly connected at the top of the first mixing cylinder, an outflow pipe is fixedly connected at the right side of the first mixing cylinder, the right end of the outflow pipe is communicated with a second mixing cylinder, a first supporting plate is fixedly connected at the bottom of the second mixing cylinder, the first supporting plate is fixedly connected on the inner side wall of the shell, a heater is fixedly connected at the top of the first supporting plate, a second stirring rod is fixedly connected inside the second mixing cylinder, a second feeding pipe is fixedly connected at the top of the second mixing cylinder, and driving motors are fixedly connected at the top ends of the first stirring rod and the second stirring rod, the driving motor is fixedly connected to the top of the shell, and an electromagnetic valve is arranged in the outflow pipe; when in work, a proper amount of water is manually added into the first mixing cylinder and the second mixing cylinder from the first feeding pipe and the second feeding pipe, then adding a proper amount of starch into the second mixing cylinder through the second feeding pipe, adding the rest proper amount of raw materials into the first mixing cylinder through the first feeding pipe, starting a driving motor above the first mixing cylinder and a heater at the bottom of the first supporting plate, heating water in the first mixing cylinder by the heater, the driving motor drives the first stirring rod to stir and mix the raw materials in the first mixing cylinder uniformly, after the raw materials in the first mixing cylinder and the second mixing cylinder are mixed uniformly primarily, discharging the raw materials in the first mixing cylinder into the second mixing cylinder from the outflow pipe, starting a driving motor above the second mixing cylinder, the driving motor drives the second stirring rod to rotate, and the preliminarily and uniformly mixed raw materials and the starch mixed solution are uniformly mixed to form the required culture solution.

As a further scheme of the invention, the belt conveying part comprises a conveying belt, a servo motor and two transmission bases, wherein each transmission base is composed of a supporting base and a transmission roller which is rotationally connected to the supporting base; during operation, the servo motor rotates to drive the conveying belt to transmit between the two transmission bases, so that the culture dish mechanism and the inclined plane top blocks on the two sides of the culture dish mechanism are in reciprocating transmission, and continuous material injection of the culture dish mechanism is realized.

As a further scheme of the invention, the culture dish mechanism comprises two arc-shaped limiting sheets symmetrically fixed on the conveying belt, and a culture dish is tightly accommodated between the two arc-shaped limiting sheets; during operation, two arc spacing pieces that the symmetry is fixed on the conveyer belt can be to culture dish position location, guarantee the precision that quantitative liquid injection portion annotated the material, guarantee can not appear spilling the material and influence the inconsistent problem of culture dish injection culture solution volume.

As a further scheme of the invention, the first reset mechanism comprises a mounting batten in a shape of a Chinese character 'ji' and a first spring, and two ends of the first spring are respectively fixed on the bottom surface of the top of the mounting batten and the top of the sliding ejector rod; during operation, after the sliding ejector rod is separated from the limiting groove, the first spring at the top of the mounting strip can push the sliding ejector rod to the initial position to be inserted and positioned with the positioning plate again, and the requirement of automatic material injection next time is met.

As a further scheme of the invention, the second reset mechanism comprises a straight guide rod and a second spring, one end of the straight guide rod is fixed on the side surface of the first scraper, the other end of the straight guide rod penetrates through the mounting plate and is in sliding connection with the mounting plate, the second spring is sleeved on the straight guide rod, and two ends of the second spring are respectively fixedly connected with the first scraper and the straight guide rod; during operation, after the sliding ejector rod is separated from the limiting groove, the first scraper can be restored to the initial position by the aid of the second spring matched with the straight guide rod, and the requirement for next automatic material injection is met.

As a further scheme of the invention, the automatic control mechanism comprises a discharging pipe, a material blocking plate, a rotating shaft, a gear, a rack rod, a limiting sleeve and a third reset mechanism for driving the rack rod to reset, the discharging pipe is fixed at the bottom of the second mixing barrel and communicated with the second mixing barrel, the rotating shaft penetrates through the discharging pipe, two ends of the rotating shaft are respectively fixedly connected with the material blocking plate and the gear, the material blocking plate is arranged in the discharging pipe and used for controlling the circulation of the discharging pipe, the limiting sleeve is fixed on the inner wall of the filtering tank and is in sliding assembly with the rack rod, a gear surface meshed with the gear is arranged at the top of the rack rod, and the top of the rack rod is attached to; the during operation, when the culture dish mechanism begins to move into the notes material position under the ration notes liquid portion, first scraper blade moves right simultaneously, move right in-process at first scraper blade, rack bar on the first scraper blade is followed and is removed, and simultaneously with the gear action, thereby the gear can produce and rotate and order about the striker plate upset, make the discharging pipe become the open mode by the closure state, carry out automatic blanking, after slip ejector pin breaks away from the spacing groove, first scraper blade resets under the effect of third canceling release mechanical system, and make the striker plate upset initial condition, make the discharging pipe become the closure state by the open mode, the automatic shutdown blanking, thereby make whole process ability ration blanking.

As a further scheme of the invention, the third reset mechanism comprises a fourth spring, and the fourth spring is fixed on the inner wall of the filter tank through a connecting lug; during operation, when the first scraper blade is resetting, the fourth spring can push the first scraper blade to return to the original position, and the requirement of automatic material injection next time is met.

As a further scheme of the invention, a material leakage prevention mechanism is arranged on the discharging pipe and comprises a sealing ring which is slidably sleeved on the outer wall of the discharging pipe, the bottom of the sealing ring is contacted with the top of the filtering tank, the front side and the rear side of the sealing ring are fixedly connected with T-shaped guide blocks, the two T-shaped guide blocks are contacted with a first scraper, the tops of the two T-shaped guide blocks are fixedly connected with two compression springs, the top ends of the two compression springs on the same side are fixedly connected with a fourth connecting plate, the fourth connecting plate is fixedly connected to the inner side wall of the side of the filtering tank, and the bottom of the filtering tank is fixedly connected with a flow guide pipe; when the culture medium automatic feeding device works, because the distance between the discharging pipe and the filter tank is the first scraping plate, the culture medium can flow out of the culture dish mechanism from the discharging pipe during each discharging, the quantity of the culture medium added into the culture dish mechanism can be inconsistent every time, the sealing ring is connected to the outer side wall of the discharging pipe in a sliding mode, when the first scraping plate is going to pass through the bottom of the discharging pipe, the first scraping plate firstly acts on the T-shaped guide block, the T-shaped guide block enables the T-shaped guide block to move upwards to drive the sealing ring to slide upwards, interference between the sealing ring and the first scraping plate is avoided, when the first scraping plate completely passes through the bottom of the discharging pipe, the first scraping plate firstly breaks away from the T-shaped guide block, the T-shaped guide block drives the sealing ring to descend to the top of the filter tank under the action of the compression spring to seal between the discharging pipe and the culture dish mechanism, and the culture medium can directly flow into, avoid the culture solution edgewise to flow, avoid the loss of culture solution, guarantee to pour into the volume of the culture solution in the culture dish mechanism unanimous at every turn, prevent that the culture dish mechanism from leading to the fact the problem production that the culture effect is different because of the volume inconsistency of culture solution.

As a further scheme of the invention, the front side and the rear side of the culture dish are fixedly connected with second guide rails, the second guide rails are connected with sealing covers in a sliding manner, the top of each sealing cover is fixedly connected with a first stop block, the right side of the first stop block is contacted with a U-shaped push plate, the U-shaped push plates are fixedly connected to the left side walls of the front sliding ejector rod and the rear sliding ejector rod, the inner side walls of the second guide rails are fixedly connected with first magnets, and the right side walls of the sealing covers are fixedly connected with second magnets; when the culture dish feeding device works, after culture liquid is added into the culture dish, the culture dish needs to be manually closed and sealed, time and labor are wasted, and when manual closing is carried out, the culture dish is easy to pollute, the culture dish is automatically sealed, personnel are not needed to participate, the culture solution is prevented from being exposed in the air, and the culture effect of the culture solution in the culture dish is guaranteed.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention is provided with the blending part, the quantitative liquid injection part, the belt conveying part, the plurality of culture dish mechanisms and the inclined plane top block, the blending part can blend raw materials as much as possible, the filter tank in the quantitative liquid injection part can be prevented from blocking a filter hole on the filter tank due to a large amount of large granular substances when filtering the culture liquid, the amount of the culture liquid added into each culture dish mechanism is not influenced, in the process that each culture dish mechanism passes through the lower part of the quantitative liquid injection part, the inclined plane top block can control the quantitative liquid injection part to inject the culture dish mechanism for a certain time, the automatic quantitative work of each culture dish mechanism is completed, meanwhile, the quantitative liquid injection part can scrape the large granular substances on the top of the filter tank by the first scraper after the automatic quantitative work of each culture dish mechanism is completed, and the problem that the filter plate of the culture liquid adding device in the prior art filters the culture liquid is solved, large granule material can be stayed at the top, can block up the filter orifice, leads to the culture solution in same time, and the culture solution that gets into the culture dish mechanism reduces, leads to the volume of adding the culture solution in the culture dish mechanism at every turn different, causes the problem that the cultivation effect of culture dish mechanism is different.

2. According to the invention, the discharge pipe of the quantitative liquid injection part is also provided with the material leakage prevention mechanism, and the material leakage prevention mechanism can seal the space between the discharge pipe and the culture dish mechanism when materials are injected each time, so that the culture liquid is prevented from flowing out from the side surface, the consistency of the amount of the culture liquid injected into the culture dish mechanism each time is ensured, and the problem of different culture effects caused by the inconsistency of the amount of the culture liquid in the culture dish mechanism is prevented.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

Fig. 1 is a first perspective cross-sectional view of the general structure of the present invention.

Fig. 2 is a second perspective cross-sectional view of the general structure of the present invention.

Fig. 3 is a schematic view of the top structure of the conveyor belt of the present invention.

FIG. 4 shows a schematic view of the tapping pipe and U-shaped filter plate according to the invention.

Fig. 5 is a schematic structural view of the first guide rail and the positioning plate of the present invention.

Fig. 6 is a schematic structural view of a discharging mechanism in the discharging pipe of the invention.

FIG. 7 is a schematic view of the structure of the U-shaped filter plate and the T-shaped guide plate of the present invention.

Fig. 8 is a sectional view showing the internal structure of the U-shaped filter plate according to the present invention.

FIG. 9 is a schematic top view of the medium of the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

the device comprises a shell 1, an L-shaped fixing plate 2, a first mixing barrel 3, a first stirring rod 4, a first feeding pipe 5, an outflow pipe 6, a second mixing barrel 7, a first supporting plate 8, a heater 9, a second stirring rod 10, a second feeding pipe 11, a driving motor 12, a discharge pipe 13, a filter tank 14, a conveying belt 15, a servo motor 16, a driving machine base 17, an arc-shaped limiting sheet 18, an inclined plane top block 19, a culture dish 20, a first scraping plate 21, a first sliding sleeve 22, a sliding opening 23, a sliding top rod 24, a first spring 25, a mounting strip plate 26, a straight guide rod 28, a mounting plate 29, a second spring 30, a first guide rail 31, a positioning plate 32, a limiting groove 33, a third spring 34, a material baffle plate 37, a rotating shaft 38, a gear 39, a rack rod 40, a limiting sleeve 41, a fourth spring 42, a connecting lug 43, a sealing ring 44, a T-shaped guide block 45, a compression spring 46, a fourth connecting plate 47, a guide, A second guide rail 49, a sealing cover 50, a first stop 51, a U-shaped push plate 52, a first magnet 53 and a second magnet 54.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-9, the present invention provides a technical solution: a mesenchymal stem cell culture medium production system comprises a blending part, wherein a quantitative liquid injection part is assembled below the blending part, a belt conveying part is arranged below the quantitative liquid injection part, a plurality of culture dish mechanisms are arranged on the belt conveying part in an array manner, and two side surfaces of each culture dish mechanism are provided with inclined plane top blocks 19 for controlling the quantitative liquid injection part to automatically inject materials quantitatively;

the quantitative liquid injection part comprises a filter tank 14, sliding openings 23 are arranged on two sides of the bottom surface of the filter tank 14, a first scraper 21 is assembled in a sliding mode through the two sliding openings 23, first sliding sleeves 22 are fixedly connected to two ends of the first scraper 21, a sliding ejector rod 24 for butting with the inclined plane ejector block 19 is assembled in each first sliding sleeve 22 in a vertical sliding mode, a first reset mechanism for resetting the sliding ejector rod 24 is arranged at the top of each first sliding sleeve 22, limiting grooves 33 are formed in the tops of the opposite side surfaces of the two sliding ejector rods 24, a positioning plate 32 is clamped and limited in the limiting grooves 33, a first guide rail 31 fixed on the side surface of the filter tank 14 is assembled in a sliding mode through the positioning plate 32, a mounting plate 29 is fixed to the top of the first guide rail 31, third springs 34 for pushing the positioning plate 32 are fixedly connected to the mounting plate 29, and a second reset mechanism for pulling the first scraper 21 to reset is further arranged on the mounting plate 29, an automatic control mechanism which is matched with the first scraper 21 to move and automatically start the material injection is also arranged in the filter tank 14;

in the prior art, after a culture solution which is stirred and mixed is filtered by a culture solution adding device, the culture solution needs to be filled into a culture medium, the current main means is that a person controls a discharge valve of a mixing barrel of the culture solution adding device to discharge, the volume of the culture solution which is added into the culture medium each time is difficult to accurately control, the addition amount of the nutrient solution in each culture medium is inconsistent, large granular substances can be remained at the top of a filter plate of the current culture solution adding device after the culture solution is filtered, a filter opening can be blocked, the culture solution which enters the culture medium is reduced in the same time, the culture solution in the culture medium is reduced, and the volume of the culture solution which is added each time is deviated, when the culture medium adding device works, firstly, a uniformly mixing part can uniformly mix the culture solution which needs to be injected into a culture dish mechanism, and uniformly mix raw materials as much as possible, when the belt conveying part conveys the culture dish mechanism and the inclined plane top block 19 on the belt conveying part to the position below the quantitative liquid injection part, the inclined plane top block 19 can act with the sliding ejector rod 24 to enable the sliding ejector rod 24 to obtain the trend of moving upwards and moving towards the right, at the moment, the third spring 34 can push the positioning plate 32 and the limiting groove 33 to be clamped together, so that the sliding ejector rod 24 is limited to move upwards, the sliding ejector rod 24 can only move rightwards under the pushing of the inclined plane top block 19, when the sliding ejector rod 24 moves rightwards, the first scraper 21 can move along with the sliding ejector rod 24 to enable the mechanism to regulate and control the quantitative liquid injection part to start to inject materials, when the sliding ejector rod 24 and the positioning plate 32 move to the end part of the first guide rail 31, the position of the positioning plate 32 is limited by the first guide rail 31, no displacement occurs, but the inclined plane top block 19 still moves rightwards continuously, after a period of time, the positioning plate 32 can be separated from the limiting groove 33 on the sliding ejector rod 24, the positioning plate 32 moves upwards under the action of the inclined plane top block 19 and gradually separates from the inclined plane top block 19, after the positioning plate 32 separates from the inclined plane top block 19, the positioning plate 32 is restored to the initial position under the action of the first reset mechanism, the limiting groove 33 on the positioning plate is clamped with the positioning plate 32 again under the action of the third spring 34, under the action of the second reset mechanism, the first scraper 21, the first sliding sleeve 22, the sliding ejector rod 24 and the positioning plate 32 are restored to the initial positions, and simultaneously the self-control mechanism is controlled to complete the stop material injection of the quantitative liquid injection part, the time of the above process to the material injection in the culture dish mechanism is consistent every time, so that each culture dish mechanism automatically injects materials quantitatively, when having solved personnel control culture solution interpolation device's mixing drum bleeder valve and having carried out the ejection of compact, be difficult to accurate hold the volume of the culture solution in adding the culture medium at every turn, lead to the inconsistent problem of nutrient solution addition in every culture medium, and simultaneously, at the in-process that first scraper blade 21 resets, first scraper blade 21 can strike off the large granule material at filter tank 14 top, the filter that has solved culture solution interpolation device among the prior art is behind the filtration culture solution, large granule material can be persisted at the top, can block up the filtration mouth, lead to the culture solution in same time, the culture solution that gets into in the culture dish mechanism reduces, the volume that leads to the culture solution in adding the culture dish mechanism at every turn is different, cause the different problem of culture effect of culture dish mechanism.

As a further scheme of the invention, the blending part comprises a shell 1, an L-shaped fixed plate 2 is fixedly connected on the inner side wall of the shell 1, a first mixing cylinder 3 is fixedly connected on the top of the L-shaped fixed plate 2, a first stirring rod 4 is rotatably connected on the top of the first mixing cylinder 3, a first feeding pipe 5 is fixedly connected on the top of the first mixing cylinder 3, an outflow pipe 6 is fixedly connected on the right side of the first mixing cylinder 3, the right end of the outflow pipe 6 is communicated with a second mixing cylinder 7, a first supporting plate 8 is fixedly connected on the bottom of the second mixing cylinder 7, the first supporting plate 8 is fixedly connected on the inner side wall of the shell 1, a heater 9 is fixedly connected on the top of the first supporting plate 8, a second stirring rod 10 is fixedly connected inside the second mixing cylinder 7, a second feeding pipe 11 is fixedly connected on the top of the second mixing cylinder 7, and driving motors 12 are fixedly connected on the top, the driving motor 12 is fixedly connected to the top of the shell 1, and the inside of the outflow pipe 6 is provided with an electromagnetic valve; when the mixing machine works, a proper amount of water is manually added into a first mixing cylinder 3 and a second mixing cylinder 7 from a first feeding pipe 5 and a second feeding pipe 11, then a proper amount of starch is added into the second mixing cylinder 7 through the second feeding pipe 11, other proper amounts of raw materials are added into the first mixing cylinder 3 from the first feeding pipe 5, then a driving motor 12 above the first mixing cylinder 3 and a heater 9 at the bottom of a first supporting plate 8 are started, the heater 9 heats the water in the first mixing cylinder 3, the driving motor 12 drives a first stirring rod 4 to stir and mix the raw materials in the first mixing cylinder 3, after the raw materials in the first mixing cylinder 3 and the second mixing cylinder 7 are preliminarily mixed, the raw materials in the first mixing cylinder 3 are discharged into the second mixing cylinder 7 from an outflow pipe 6, at the moment, the driving motor 12 above the second mixing cylinder 7 is started, and the driving motor 12 drives a second stirring rod 10 to rotate, and uniformly mixing the primarily uniformly mixed raw materials and the starch mixed solution to obtain the required culture solution.

As a further scheme of the invention, the belt conveying part comprises a conveying belt 15, a servo motor 16 and two driving bases 17, wherein the driving bases 17 are composed of supporting bases and driving rollers rotatably connected to the supporting bases, the conveying belt 15 is sleeved on the driving rollers on the two driving bases 17, the servo motor 16 is fixedly arranged on the supporting base of one of the driving bases 17, and an output shaft of the servo motor is fixedly connected with the driving rollers on the driving bases 17; during operation, the servo motor 16 rotates to drive the conveyer belt 15 to transmit between the two transmission bases 17, so that the culture dish mechanism and the inclined plane top blocks 19 on the two sides of the culture dish mechanism are in reciprocating transmission, and continuous material injection of the culture dish mechanism is realized.

As a further scheme of the invention, the culture dish mechanism comprises two arc-shaped limiting pieces 18 which are symmetrically fixed on the conveying belt 15, and a culture dish 20 is tightly accommodated between the two arc-shaped limiting pieces 18; during operation, two arc spacing pieces 18 that the symmetry is fixed on conveyer belt 15 can be to 20 position location of culture dish, guarantee the precision of quantitative liquid injection portion notes material, guarantee can not appear spilling the material and influence the problem that culture dish 20 pours into the culture solution volume inconsistent.

As a further scheme of the invention, the first reset mechanism comprises a 7-shaped mounting strip plate 26 and a first spring 25, wherein two ends of the first spring 25 are respectively fixed on the bottom surface of the top of the mounting strip plate 26 and the top of the sliding ejector rod 24; during operation, after the sliding ejector rod 24 is separated from the limiting groove 33, the first spring 25 at the top of the mounting strip 26 pushes the sliding ejector rod 24 to the initial position to be inserted and positioned with the positioning plate 32 again, and the requirement of automatic material injection next time is met.

As a further scheme of the present invention, the second reset mechanism includes a straight guide rod 28 and a second spring 30, one end of the straight guide rod 28 is fixed on the side surface of the first scraper 21, the other end of the straight guide rod 28 penetrates through the mounting plate 29 and is slidably connected therewith, the second spring 30 is sleeved on the straight guide rod 28, and two ends of the second spring 30 are respectively fixedly connected with the first scraper 21 and the straight guide rod 28; during operation, after the sliding ejector rod 24 is separated from the limiting groove 33, the second spring 30 is matched with the straight guide rod 28 to restore the first scraper 21 to the initial position, so that the requirement of automatic material injection next time is met.

As a further scheme of the invention, the automatic control mechanism comprises a discharge pipe 13, a material baffle plate 37, a rotating shaft 38, a gear 39, a rack rod 40, a limiting sleeve 41 and a third reset mechanism for driving the rack rod 40 to reset, the discharge pipe 13 is fixed at the bottom of the second mixing drum 7 and communicated with the second mixing drum, the rotating shaft 38 penetrates through the discharge pipe 13, two ends of the rotating shaft are respectively fixedly connected with the material baffle plate 37 and the gear 39, the material baffle plate 37 is arranged inside the discharge pipe 13 and used for controlling the circulation of the discharge pipe 13, the limiting sleeve 41 is fixed on the inner wall of the filter tank 14 and is assembled with the rack rod 40 in a sliding manner, the top of the rack rod 40 is provided with a gear surface meshed with the gear 39, and the top is; during operation, when the culture dish mechanism begins to move into the material injection position under the quantitative liquid injection part, first scraper 21 moves rightwards simultaneously, in the process that first scraper 21 moves rightwards, rack bar 40 on first scraper 21 moves with following, and simultaneously acts on with gear 39, gear 39 can produce and rotate thereby order about striker plate 37 upset, make discharging pipe 13 become open state by the closure state, carry out automatic blanking, after slip ejector pin 24 breaks away from spacing groove 33, first scraper 21 resets under the effect of third canceling release mechanical system, and make striker plate 37 upset be initial condition, make discharging pipe 13 become the closure state by the opening state, the automatic shutdown blanking, thereby make whole process ability ration blanking.

As a further scheme of the present invention, the third return mechanism comprises a fourth spring 42, the fourth spring 42 is fixed on the inner wall of the filter tank 14 through a connecting lug 43; in operation, when the first scraper 21 is in reset, the fourth spring 42 will push the first scraper to return to the original position, so as to meet the requirement of automatic material injection next time.

As a further scheme of the invention, a material leakage prevention mechanism is arranged on the discharge pipe 13 and comprises a sealing ring 44 which is slidably sleeved on the outer wall of the discharge pipe 13, the bottom of the sealing ring 44 is in contact with the top of the filter tank 14, the front side and the rear side of the sealing ring 44 are fixedly connected with T-shaped guide blocks 45, the two T-shaped guide blocks 45 are in contact with the first scraper 21, the tops of the two T-shaped guide blocks 45 are fixedly connected with two compression springs 46, the top ends of the two compression springs 46 on the same side are fixedly connected with a fourth connecting plate 47, the fourth connecting plate 47 is fixedly connected on the inner side wall of the side of the filter tank 14, and the bottom of the filter tank; in operation, because there is a distance of the first scraper 21 between the discharging pipe 13 and the filtering tank 14, during each discharging, the culture fluid may flow from the discharging pipe 13 to the outside of the culture dish mechanism, which may cause the amount of the culture fluid added to the culture dish mechanism to be inconsistent each time, by slidably connecting the sealing ring 44 to the outer side wall of the discharging pipe 13, when the first scraper 21 is about to pass through the bottom of the discharging pipe 13, the first scraper 21 first acts on the T-shaped guide block 45, the T-shaped guide block 45 makes it move upwards, which drives the sealing ring 44 to slide upwards, so as to avoid the interference between the sealing ring 44 and the first scraper 21, after the first scraper 21 completely passes through the bottom of the discharging pipe 13, the first scraper 21 first disengages from the T-shaped guide block 45, the T-shaped guide block 45 drives the sealing ring 44 to descend to the top of the filtering tank 14 under the action of the compression spring 46, so as to seal between the discharging pipe 13 and the culture dish mechanism, realize that the culture solution can both be followed discharging pipe 13 at every turn and directly flow in the culture dish mechanism, avoid the culture solution edgewise to flow, avoid the loss of culture solution, guarantee to pour into the volume of the culture solution in the culture dish mechanism unanimous at every turn, prevent that the culture dish mechanism from leading to the fact the problem production that the culture effect is different because of the volume inconsistency of culture solution.

As a further scheme of the invention, the front side and the back side of the culture dish 20 are fixedly connected with a second guide rail 49, the second guide rail 49 is connected with a sealing cover 50 in a sliding way, the top of the sealing cover 50 is fixedly connected with a first stop dog 51, the right side of the first stop dog 51 is contacted with a U-shaped push plate 52, the U-shaped push plate 52 is fixedly connected on the left side wall of the front sliding ejector rod 24 and the back sliding ejector rod 24, the inner side wall of the second guide rail 49 is fixedly connected with a first magnet 53, and the right side wall of the sealing cover 50 is; in operation, after the culture dish 20 is filled with culture fluid, the culture dish 20 needs to be manually covered and sealed, which is time-consuming and labor-consuming, and when manual covering is performed, the culture dish 20 is easily polluted, the sealing cover 50 is connected to the top of the culture dish 20 in a sliding manner, when the culture dish 20 moves towards the bottom of the discharge pipe 13, the U-shaped push plate 52 acts on the first stop 51, the sealing cover 50 is slowly opened, two first magnets 53 and two second magnets 54, which are attracted together, of the sealing cover 50 are separated, the sealing cover 50 slides along the second guide rail 49 to be opened and moves to the right side of the discharge pipe 13, so that the interference of the culture fluid entering the culture dish 20 is avoided, when the feeding is completed, the sliding push rod 24 moves upwards to drive the U-shaped push plate 52 to move upwards to separate from the first stop 51, the sealing cover 50 reseals the culture dish 20 under the attraction force of the first magnets 53 and the second magnets 54, and, the automatic sealed lid 50 of will opening, reinforced completion back, automatic sealed culture dish 20 need not personnel to participate in, avoids the culture solution to expose in the air, guarantees the cultivation effect of culture solution in the culture dish 20 of cultivateing.

The working principle is as follows: when the culture medium automatic feeding device works, firstly, the mixing part can uniformly mix the culture medium needing to be fed into the culture dish mechanism completely, the mixing part can uniformly mix the raw materials as much as possible, the filter tank 14 is prevented from blocking a filter hole on the filter tank 14 due to a large amount of large particles when the culture medium is filtered, the quantity of the culture medium fed into the culture dish mechanism every time is influenced, when the belt conveying part conveys the culture dish mechanism and the inclined plane top block 19 on the belt conveying part to the position below the quantitative liquid feeding part, the inclined plane top block 19 can act with the sliding ejector rod 24, so that the sliding ejector rod 24 obtains the upward movement and rightward movement trends, at the moment, the third spring 34 can push the positioning plate 32 and the limiting groove 33 to be clamped together, so as to limit the upward movement of the sliding ejector rod 24, so that the sliding ejector rod 24 can only move rightwards under the pushing of the inclined plane top block 19, when the sliding ejector rod 24 moves rightwards, the first scraper 21 can move together with the, when the sliding ejector rod 24 and the positioning plate 32 move to the end of the first guide rail 31, the position of the positioning plate 32 is limited by the first guide rail 31, and displacement does not occur, but the inclined plane top block 19 still moves rightwards continuously, after a period of time, the positioning plate 32 can be separated from the limiting groove 33 on the sliding ejector rod 24, the positioning plate 32 moves upwards under the action of the inclined plane top block 19 and gradually separates from the inclined plane top block 19, after the positioning plate 32 separates from the inclined plane top block 19, the positioning plate 32 recovers to the initial position under the action of the first reset mechanism, the limiting groove 33 on the positioning plate is clamped with the positioning plate 32 again under the action of the third spring 34, under the action of the second reset mechanism, the first scraper 21, the first sliding sleeve 22, the sliding ejector rod 24 and the positioning plate 32 recover to the initial positions, and the self-control mechanism is controlled to complete the stop of material injection of the quantitative liquid injection part, and the time of material injection into the culture dish mechanism is consistent each time in the previous process, so the automatic material of annotating of each culture dish mechanism is all quantitative, when the mixing drum bleeder valve of personnel's control culture solution interpolation device had been solved and the ejection of compact, be difficult to accurate hold the volume of the culture solution in the culture medium of adding at every turn, lead to the inconsistent problem of nutrient solution addition in every culture medium, and simultaneously, at the in-process that first scraper blade 21 resets, first scraper blade 21 can strike off the large granule material at 14 tops of filter tank, the filter of having solved culture solution interpolation device among the prior art is behind the filtration culture solution, large granule material can be persisted at the top, can block up the filtration mouth, lead to the culture solution in same time, the culture solution that gets into in the culture dish mechanism reduces, the volume that leads to adding the culture solution in the culture dish mechanism at every turn is different, the different problem of culture effect that causes the culture dish mechanism.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. The utility model provides a mesenchymal stem cell culture medium production system, includes mixing portion, its characterized in that: a quantitative liquid injection part is assembled below the blending part, a belt conveying part is arranged below the quantitative liquid injection part, a plurality of culture dish mechanisms are arranged on the belt conveying part in an array manner, and two side surfaces of each culture dish positioning mechanism are provided with inclined plane top blocks (19) for controlling the quantitative liquid injection part to automatically inject materials quantitatively;

the quantitative liquid injection part comprises a filter tank (14), sliding openings (23) are formed in two sides of the bottom surface of the filter tank (14), two sliding openings (23) are assembled with first scraping plates (21) in a sliding mode, two ends of each first scraping plate (21) are fixedly connected with first sliding sleeves (22), a sliding ejector rod (24) used for being in butt joint with an inclined plane ejector block (19) is vertically assembled in each first sliding sleeve (22) in a sliding mode, a first reset mechanism used for resetting the sliding ejector rod (24) is arranged at the top of each first sliding sleeve (22), limiting grooves (33) are formed in the tops of the opposite sides of the two sliding ejector rods (24), a limiting positioning plate (32) is clamped in each limiting groove (33), a first guide rail (31) fixed on the side surface of the filter tank (14) is assembled in a sliding mode, and a mounting plate (29) is fixed to the tops of the first guide rails (31), equal fixedly connected with is used for promoting third spring (34) of locating plate (32) on mounting panel (29), and still is equipped with the second canceling release mechanical system that is used for pulling first scraper blade (21) and resets on mounting panel (29), still be equipped with the automatic self-control mechanism who opens the notes material of cooperation first scraper blade (21) removal in filter-tank (14).

2. A mesenchymal stem cell culture medium production system according to claim 1, wherein: the mixing part comprises a shell (1), an L-shaped fixing plate (2) is fixedly connected to the inner side wall of the shell (1), a first mixing barrel (3) is fixedly connected to the top of the L-shaped fixing plate (2), a first stirring rod (4) is rotatably connected to the top of the first mixing barrel (3), a first inlet pipe (5) is fixedly connected to the top of the first mixing barrel (3), an outlet pipe (6) is fixedly connected to the right side of the first mixing barrel (3), a second mixing barrel (7) is communicated with the right end of the outlet pipe (6), a first supporting plate (8) is fixedly connected to the bottom of the second mixing barrel (7), the first supporting plate (8) is fixedly connected to the inner side wall of the shell (1), a heater (9) is fixedly connected to the top of the first supporting plate (8), and a second stirring rod (10) is fixedly connected to the inside of the second mixing barrel (7), and second mixing cylinder (7) top fixedly connected with second inlet pipe (11), first puddler (4) with the equal fixedly connected with driving motor (12) in second puddler (10) top, driving motor (12) fixed connection is at the top of shell (1), outflow pipe (6) inside is provided with the solenoid valve.

3. A mesenchymal stem cell culture medium production system according to claim 1, wherein: the belt conveying part comprises a conveying belt (15), a servo motor (16) and two driving bases (17), the driving bases (17) are formed by supporting seats and driving rollers connected to the supporting seats in a rotating mode, the conveying belt (15) is sleeved on the driving rollers on the two driving bases (17), the servo motor (16) is fixedly installed on one of the supporting seats of the driving bases (17), and output shafts of the servo motor are fixedly connected with the driving rollers on the driving bases (17).

4. A mesenchymal stem cell culture medium production system according to claim 3, wherein: the culture dish mechanism comprises two arc-shaped limiting pieces (18) which are symmetrically fixed on the conveying belt (15), and a culture dish (20) is tightly contained between the two arc-shaped limiting pieces (18).

5. A mesenchymal stem cell culture medium production system according to claim 1, wherein: the first reset mechanism comprises a 7-shaped installation strip plate (26) and a first spring (25), and two ends of the first spring (25) are respectively fixed to the bottom surface of the top of the installation strip plate (26) and the top of the sliding ejector rod (24).

6. A mesenchymal stem cell culture medium production system according to claim 1, wherein: the second reset mechanism comprises a straight guide rod (28) and a second spring (30), one end of the straight guide rod (28) is fixed on the side face of the first scraper (21), the other end of the straight guide rod (28) penetrates through the mounting plate (29) and is in sliding connection with the mounting plate, the second spring (30) is sleeved on the straight guide rod (28), and two ends of the second spring (30) are respectively fixedly connected with the first scraper (21) and the straight guide rod (28).

7. A mesenchymal stem cell culture medium production system according to claim 2, wherein: from control mechanism includes discharging pipe (13), striker plate (37), axis of rotation (38), gear (39), rack bar (40), stop collar (41) and is used for driving the third canceling release mechanical system that rack bar (40) reset, discharging pipe (13) are fixed in second mixing drum (7) bottom and rather than the intercommunication, axis of rotation (38) run through discharging pipe (13) and both ends respectively with striker plate (37) and gear (39) fixed connection, striker plate (37) set up the circulation that is used for controlling discharging pipe (13) in discharging pipe (13) inside, stop collar (41) are fixed on filter tank (14) inner wall and with rack bar (40) sliding assembly, rack bar (40) top is equipped with the gear face with gear (39) meshing, and the top forms with it with first scraper blade (21) laminating and blocks.

8. A mesenchymal stem cell culture medium production system according to claim 7, wherein: the third reset mechanism comprises a fourth spring (42), and the fourth spring (42) is fixed on the inner wall of the filter tank (14) through a connecting lug (43).

9. A mesenchymal stem cell culture medium production system according to claim 7, wherein: be equipped with leak protection material mechanism on discharging pipe (13), leak protection material mechanism includes sealing ring (44) of slip suit on discharging pipe (13) outer wall, sealing ring (44) bottom contacts with filter-tank (14) top, the equal fixedly connected with T type guide block (45) in both sides around sealing ring (44), two T type guide block (45) contact with first scraper blade (21), two T type guide block (45) top fixedly connected with two compression spring (46), the homonymy is two compression spring (46) top fixedly connected with fourth connecting plate (47), fourth connecting plate (47) fixed connection is on filter-tank (14) side inside wall, filter-tank (14) bottom fixedly connected with honeycomb duct (48).

10. A mesenchymal stem cell culture medium production system according to claim 4, wherein: both sides fixedly connected with second guide rail (49) around culture dish (20), sliding connection has sealed lid (50) in second guide rail (49), sealed lid (50) first dog of top fixedly connected with (51), first dog (51) right side contact has U type push pedal (52), U type push pedal (52) fixed connection is on two slip ejector pin (24) side left side walls in the front and back, first magnet (53) of fixedly connected with on second guide rail (49) inside wall, fixedly connected with second magnet (54) on sealed lid (50) right side wall.