CN112469733A - Mart-1(27-35)表位特异性t细胞受体 - Google Patents
Mart-1(27-35)表位特异性t细胞受体 Download PDFInfo
- Publication number
- CN112469733A CN112469733A CN201880095200.6A CN201880095200A CN112469733A CN 112469733 A CN112469733 A CN 112469733A CN 201880095200 A CN201880095200 A CN 201880095200A CN 112469733 A CN112469733 A CN 112469733A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- seq
- amino acid
- sequence
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101000578784 Homo sapiens Melanoma antigen recognized by T-cells 1 Proteins 0.000 title claims abstract description 52
- 102100028389 Melanoma antigen recognized by T-cells 1 Human genes 0.000 title claims abstract description 51
- 108091008874 T cell receptors Proteins 0.000 title claims abstract description 31
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 title claims abstract description 30
- 210000004027 cell Anatomy 0.000 claims abstract description 73
- 210000001744 T-lymphocyte Anatomy 0.000 claims abstract description 36
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 17
- 108010047041 Complementarity Determining Regions Proteins 0.000 claims abstract description 10
- 108020004707 nucleic acids Proteins 0.000 claims description 36
- 102000039446 nucleic acids Human genes 0.000 claims description 36
- 150000007523 nucleic acids Chemical class 0.000 claims description 36
- 239000013598 vector Substances 0.000 claims description 23
- 201000001441 melanoma Diseases 0.000 claims description 17
- 125000000539 amino acid group Chemical group 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 5
- 238000011282 treatment Methods 0.000 claims description 5
- 239000008194 pharmaceutical composition Substances 0.000 claims description 3
- 108091026890 Coding region Proteins 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 238000011321 prophylaxis Methods 0.000 claims 3
- 108010075254 C-Peptide Proteins 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 43
- 229920001184 polypeptide Polymers 0.000 abstract description 42
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 42
- 102100037850 Interferon gamma Human genes 0.000 abstract description 4
- 108010074328 Interferon-gamma Proteins 0.000 abstract description 4
- 238000009509 drug development Methods 0.000 abstract description 2
- 239000003596 drug target Substances 0.000 abstract description 2
- 230000009258 tissue cross reactivity Effects 0.000 description 20
- 239000000203 mixture Substances 0.000 description 17
- 238000006243 chemical reaction Methods 0.000 description 15
- 238000002156 mixing Methods 0.000 description 11
- 239000000427 antigen Substances 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 239000002609 medium Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 230000000638 stimulation Effects 0.000 description 9
- 239000000178 monomer Substances 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 238000003556 assay Methods 0.000 description 7
- 238000007664 blowing Methods 0.000 description 7
- 238000001962 electrophoresis Methods 0.000 description 7
- 238000011068 loading method Methods 0.000 description 7
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 7
- 239000012146 running buffer Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 5
- GUAHPAJOXVYFON-ZETCQYMHSA-N (8S)-8-amino-7-oxononanoic acid zwitterion Chemical compound C[C@H](N)C(=O)CCCCCC(O)=O GUAHPAJOXVYFON-ZETCQYMHSA-N 0.000 description 5
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 5
- 230000003321 amplification Effects 0.000 description 5
- 230000006037 cell lysis Effects 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000011324 bead Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 4
- 238000010839 reverse transcription Methods 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 230000001502 supplementing effect Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- NEHKZPHIKKEMAZ-ZFVKSOIMSA-N (2s)-2-[[(2s,3r)-2-[[(2s)-2-[[(2s,3s)-2-[[2-[[(2s,3s)-2-[[2-[[(2s)-2-[[(2s)-2-azaniumylpropanoyl]amino]propanoyl]amino]acetyl]amino]-3-methylpentanoyl]amino]acetyl]amino]-3-methylpentanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-methylb Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O NEHKZPHIKKEMAZ-ZFVKSOIMSA-N 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 229920001917 Ficoll Polymers 0.000 description 3
- 108010010995 MART-1 Antigen Proteins 0.000 description 3
- 102000016200 MART-1 Antigen Human genes 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 239000003161 ribonuclease inhibitor Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- 239000013603 viral vector Substances 0.000 description 3
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
- 238000011510 Elispot assay Methods 0.000 description 2
- 108090000172 Interleukin-15 Proteins 0.000 description 2
- 108010002350 Interleukin-2 Proteins 0.000 description 2
- 108010002586 Interleukin-7 Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108091093126 WHP Posttrascriptional Response Element Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229960003237 betaine Drugs 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 238000003114 enzyme-linked immunosorbent spot assay Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000010926 purge Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
- 102000017420 CD3 protein, epsilon/gamma/delta subunit Human genes 0.000 description 1
- 210000001239 CD8-positive, alpha-beta cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000009946 DNA mutation Effects 0.000 description 1
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 1
- 102000011786 HLA-A Antigens Human genes 0.000 description 1
- 108010075704 HLA-A Antigens Proteins 0.000 description 1
- 102000025850 HLA-A2 Antigen Human genes 0.000 description 1
- 108010074032 HLA-A2 Antigen Proteins 0.000 description 1
- 101000599940 Homo sapiens Interferon gamma Proteins 0.000 description 1
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 102000004388 Interleukin-4 Human genes 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 101800001494 Protease 2A Proteins 0.000 description 1
- 101800001066 Protein 2A Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 101100043635 Solanum tuberosum SS2 gene Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 210000003161 choroid Anatomy 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 208000035250 cutaneous malignant susceptibility to 1 melanoma Diseases 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 231100000075 skin burn Toxicity 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/7051—T-cell receptor (TcR)-CD3 complex
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/463—Cellular immunotherapy characterised by recombinant expression
- A61K39/4632—T-cell receptors [TCR]; antibody T-cell receptor constructs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/4644—Cancer antigens
- A61K39/46449—Melanoma antigens
- A61K39/464491—Melan-A/MART
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/867—Retroviral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Oncology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
提供一种MART‑1(27‑35)表位特异性T细胞受体,该T细胞受体包含α链和β链;所述α链包含三个互补决定区,序列分别为SEQ ID No.3的第61‑66位、第84‑89位以及第124‑136位;所述β链包含三个互补决定区,氨基酸序列分别为SEQ ID No.4的第46‑50位、第68‑73位以及第112‑125位。提供表达TCR的T细胞可以有效的识别T2细胞负载的MART‑1(27‑35)表位多肽,并分泌IFN‑γ,证明其具有功能。该TCR与相关药物靶标联用,可以有效的进行药物开发。
Description
PCT国内申请,说明书已公开。
Claims (15)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2018/095395 WO2020010565A1 (zh) | 2018-07-12 | 2018-07-12 | Mart-1(27-35)表位特异性t细胞受体 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112469733A true CN112469733A (zh) | 2021-03-09 |
CN112469733B CN112469733B (zh) | 2022-04-19 |
Family
ID=69142897
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201880095200.6A Active CN112469733B (zh) | 2018-07-12 | 2018-07-12 | Mart-1(27-35)表位特异性t细胞受体 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20220340638A1 (zh) |
CN (1) | CN112469733B (zh) |
WO (1) | WO2020010565A1 (zh) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008039818A2 (en) * | 2006-09-26 | 2008-04-03 | Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Modified t cell receptors and related materials and methods |
WO2008042814A2 (en) * | 2006-09-29 | 2008-04-10 | California Institute Of Technology | Mart-1 t cell receptors |
CN101415827A (zh) * | 2005-12-09 | 2009-04-22 | 阿哥斯医疗公司 | 产生抗原特异性效应t细胞的方法 |
CN105821080A (zh) * | 2016-05-06 | 2016-08-03 | 深圳精准医疗科技有限公司 | 一种提升安全性、用于表达密码子优化的Anti-MART-1 TCR基因的慢病毒载体的制备及其应用 |
CN106478809A (zh) * | 2015-11-06 | 2017-03-08 | 广州市香雪制药股份有限公司 | 识别prame抗原短肽的tcr |
WO2017193104A1 (en) * | 2016-05-06 | 2017-11-09 | Fred Hutchinson Cancer Research Center | T-cell immunotherapy specific for mart-1 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX343633B (es) * | 2007-03-05 | 2016-11-11 | Int Inst Cancer Immunology Inc | Gen receptor de celulas t especificas de antigeno cancerigeno, peptido codificado por el gen, y uso de los mismos. |
US8603810B2 (en) * | 2008-05-09 | 2013-12-10 | Agency For Science, Technology And Research | T-cell having a T-cell receptor that recognizes epitope HBC18-27 of hepatitis B viral core antigen |
TWI840351B (zh) * | 2018-04-05 | 2024-05-01 | 美商奇諾治療有限公司 | T細胞受體及表現其之工程化細胞 |
-
2018
- 2018-07-12 CN CN201880095200.6A patent/CN112469733B/zh active Active
- 2018-07-12 WO PCT/CN2018/095395 patent/WO2020010565A1/zh active Application Filing
- 2018-07-12 US US17/259,584 patent/US20220340638A1/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101415827A (zh) * | 2005-12-09 | 2009-04-22 | 阿哥斯医疗公司 | 产生抗原特异性效应t细胞的方法 |
WO2008039818A2 (en) * | 2006-09-26 | 2008-04-03 | Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Modified t cell receptors and related materials and methods |
WO2008042814A2 (en) * | 2006-09-29 | 2008-04-10 | California Institute Of Technology | Mart-1 t cell receptors |
CN106478809A (zh) * | 2015-11-06 | 2017-03-08 | 广州市香雪制药股份有限公司 | 识别prame抗原短肽的tcr |
CN105821080A (zh) * | 2016-05-06 | 2016-08-03 | 深圳精准医疗科技有限公司 | 一种提升安全性、用于表达密码子优化的Anti-MART-1 TCR基因的慢病毒载体的制备及其应用 |
WO2017193104A1 (en) * | 2016-05-06 | 2017-11-09 | Fred Hutchinson Cancer Research Center | T-cell immunotherapy specific for mart-1 |
Non-Patent Citations (2)
Title |
---|
LAURA A. JOHNSON等: "Gene Transfer of Tumor-Reactive TCR Confers Both High Avidity and Tumor Reactivity to Nonreactive Peripheral Blood Mononuclear Cells and Tumor-Infiltrating Lymphocytes", 《THE JOURNAL OF IMMUNOLOGY》 * |
PAUL F. ROBBINS等: "Single and Dual Amino Acid Substitutions in TCR CDRs Can Enhance Antigen-Specific T Cell Functions", 《THE JOURNAL OF IMMUNOLOGY》 * |
Also Published As
Publication number | Publication date |
---|---|
WO2020010565A1 (zh) | 2020-01-16 |
US20220340638A1 (en) | 2022-10-27 |
CN112469733B (zh) | 2022-04-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105777911B (zh) | 抗bcma嵌合抗原受体、编码基因、重组表达载体及其构建方法和应用 | |
CN108409840B (zh) | 抗cd123单链抗体及其组合的嵌合抗原受体和应用 | |
CN104910278B (zh) | 一种用于制备cart细胞的具有高效转染能力和生物学活性的慢病毒 | |
CN105820254B (zh) | 抗cd138嵌合抗原受体、编码基因、重组表达载体及其构建方法和应用 | |
Niu et al. | Preclinical evaluation of HIV-1 therapeutic ex vivo dendritic cell vaccines expressing consensus Gag antigens and conserved Gag epitopes | |
CN105950663B (zh) | 一种靶向cd30的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用 | |
CN114230658A (zh) | 新型冠状病毒特异性t细胞受体和其用途 | |
CN112251412A (zh) | 一种靶向bcma的嵌合抗原受体t细胞及其应用 | |
Jiang et al. | Rapid generation of genetically engineered T cells for the treatment of virus‐related cancers | |
WO2022012198A1 (zh) | 一种用于杀伤肿瘤的tcr-t细胞及其制备方法和应用 | |
CN107286246B (zh) | 治疗脑胶质瘤的嵌合抗原受体修饰的树突状细胞及其制备方法 | |
CN112469733B (zh) | Mart-1(27-35)表位特异性t细胞受体 | |
CN113423724B (zh) | Ebv表位高亲和力t细胞受体 | |
CN112940109B (zh) | 识别ebv抗原的t细胞受体及其应用 | |
EP4267173A2 (en) | Mage-b2-specific t-cell receptors | |
WO2020164167A1 (zh) | 一种用于通用型car-t制备的重组腺相关病毒载体及其构建方法和应用 | |
CN111484561A (zh) | 一种靶向于cd19分子的嵌合抗原受体 | |
EP4415761A1 (en) | Compositions and methods for use of recombinant t cell receptors against claudin 6 | |
CN116813744B (zh) | 一种识别mage-a3抗原短肽的t细胞受体及其应用 | |
CN117736340B (zh) | 一种靶向cll-1和nkg2dl的嵌合抗原受体nk细胞的构建及应用 | |
WO2018032619A1 (zh) | 可溶性蛋白baff在b细胞体外培养及扩增的应用 | |
CN111647606B (zh) | 靶向afp全抗原的dc细胞、ctl细胞及其制备方法和应用 | |
CN113249331B (zh) | 负载Tax抗原的DC细胞、CTL细胞及其制备方法和应用 | |
CN116063577A (zh) | Tcr或其抗原结合片段及其应用 | |
CN116769017A (zh) | Mart-1(27-35)表位特异性t细胞受体及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |