CN112458129B - Preparation process of short-chain ganoderan sulfuric acid derivative - Google Patents
Preparation process of short-chain ganoderan sulfuric acid derivative Download PDFInfo
- Publication number
- CN112458129B CN112458129B CN202011590143.4A CN202011590143A CN112458129B CN 112458129 B CN112458129 B CN 112458129B CN 202011590143 A CN202011590143 A CN 202011590143A CN 112458129 B CN112458129 B CN 112458129B
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- China
- Prior art keywords
- ganoderma lucidum
- chain
- short
- polysaccharide
- ganoderan
- Prior art date
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- 150000003467 sulfuric acid derivatives Chemical class 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 143
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 143
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 107
- 239000005017 polysaccharide Substances 0.000 claims abstract description 107
- 150000004676 glycans Chemical class 0.000 claims abstract description 104
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 66
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 21
- 241000222336 Ganoderma Species 0.000 claims abstract description 18
- 238000001212 derivatisation Methods 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims description 74
- 230000004151 fermentation Effects 0.000 claims description 74
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 37
- 238000004108 freeze drying Methods 0.000 claims description 28
- 238000001556 precipitation Methods 0.000 claims description 19
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 239000008213 purified water Substances 0.000 claims description 18
- 238000005406 washing Methods 0.000 claims description 18
- 238000000605 extraction Methods 0.000 claims description 13
- 239000000047 product Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000002518 antifoaming agent Substances 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 9
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 9
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 230000001376 precipitating effect Effects 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- LNOPIUAQISRISI-UHFFFAOYSA-N n'-hydroxy-2-propan-2-ylsulfonylethanimidamide Chemical compound CC(C)S(=O)(=O)CC(N)=NO LNOPIUAQISRISI-UHFFFAOYSA-N 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 244000144972 livestock Species 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000009304 pastoral farming Methods 0.000 claims 1
- 238000009374 poultry farming Methods 0.000 claims 1
- 230000004071 biological effect Effects 0.000 abstract description 6
- 244000144977 poultry Species 0.000 abstract description 6
- 210000004185 liver Anatomy 0.000 abstract description 5
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 4
- 230000002155 anti-virotic effect Effects 0.000 abstract description 3
- 230000003832 immune regulation Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- -1 polysaccharide sulfuric acid derivative Chemical class 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000000502 dialysis Methods 0.000 description 7
- 241000287828 Gallus gallus Species 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 229920002307 Dextran Polymers 0.000 description 5
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 5
- 235000013594 poultry meat Nutrition 0.000 description 5
- 230000003078 antioxidant effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 3
- 229910001626 barium chloride Inorganic materials 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000001937 non-anti-biotic effect Effects 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003471 anti-radiation Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 230000019635 sulfation Effects 0.000 description 1
- 238000005670 sulfation reaction Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Example 3 | Example 4 | Example 5 | Comparative example 1 | Comparative example 2 | |
Dialysis bag | 0.3567 | 0.3426 | 0.3215 | 0.3357 | 0.0072 |
Outside the dialysis bag | 0.0057 | 0.0090 | 0.0032 | 0.0055 | 0.0084 |
Grouping | Group A | Group B | Group C | Group D | Group E |
Size of ratio/%) | 0.77 | 0.88 | 0.82 | 0.37 | 0.23 |
Grouping | Group A | Group B | Group C | Group D | Group E |
SOD(U/mg) | 18.6 | 19.3 | 17.7 | 11.7 | 10.5 |
GSH(ng/mg) | 105.6 | 111.2 | 94.3 | 73.4 | 78.2 |
Claims (8)
Priority Applications (1)
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CN202011590143.4A CN112458129B (en) | 2020-12-29 | 2020-12-29 | Preparation process of short-chain ganoderan sulfuric acid derivative |
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CN202011590143.4A CN112458129B (en) | 2020-12-29 | 2020-12-29 | Preparation process of short-chain ganoderan sulfuric acid derivative |
Publications (2)
Publication Number | Publication Date |
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CN112458129A CN112458129A (en) | 2021-03-09 |
CN112458129B true CN112458129B (en) | 2022-02-15 |
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CN202011590143.4A Active CN112458129B (en) | 2020-12-29 | 2020-12-29 | Preparation process of short-chain ganoderan sulfuric acid derivative |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114903101A (en) * | 2022-05-27 | 2022-08-16 | 内蒙古阿迪斯蒙医药有限公司 | Double-jujube lucid ganoderma tea and preparation method thereof |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR0156986B1 (en) * | 1995-02-20 | 1998-10-15 | 이신영 | Preparation process of polysaccharide by cultivating ganoderma lucidum |
CN101157732A (en) * | 2007-11-12 | 2008-04-09 | 浙江大学 | Meat lucid ganoderma polysaccharide sodium sulphate and method for making same |
CN101560260A (en) * | 2009-02-26 | 2009-10-21 | 浙江大学 | Technique for extracting polysaccharide in glossy ganoderma mycelium cell and method thereof |
CN102972211A (en) * | 2012-12-21 | 2013-03-20 | 徐州工程学院 | Method for increasing growth speed of ganoderma lucidum mycelia and liquid fermentation biomass |
CN104292357B (en) * | 2014-11-11 | 2017-01-25 | 牛睦元 | Extraction method of ganoderma lucidum polysaccharide |
TWI673058B (en) * | 2018-07-20 | 2019-10-01 | 金穎生物科技股份有限公司 | Ganoderma lucidum complex polysaccharide composition |
CN109234331A (en) * | 2018-08-02 | 2019-01-18 | 宁德师范学院 | A kind of Ganoderma lucidum submerged fermentation is naturally cultivated and tunning complete utilization method |
CN111118083A (en) * | 2018-11-01 | 2020-05-08 | 王昌纬 | Method for extracting ganoderma lucidum polysaccharide |
CN110564664B (en) * | 2019-09-23 | 2023-02-21 | 桂林理工大学 | Application of sulfated xylan derivative in promoting proliferation of probiotics in vitro |
CN111620959A (en) * | 2020-06-08 | 2020-09-04 | 广州颜如玉生物科技有限公司 | Preparation method and application of ganoderma lucidum mycelium polysaccharide |
CN111533823A (en) * | 2020-06-08 | 2020-08-14 | 广州颜如玉生物科技有限公司 | Process for extracting polysaccharide from ganoderma lucidum mycelia |
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2020
- 2020-12-29 CN CN202011590143.4A patent/CN112458129B/en active Active
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation of a Short Chain Ganoderma Polysaccharide Sulfuric Acid Derivative Effective date of registration: 20221212 Granted publication date: 20220215 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010165 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20220215 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010165 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation process of a short chain Ganoderma lucidum polysaccharide sulfate derivative Granted publication date: 20220215 Pledgee: China Construction Bank Co.,Ltd. Jimo Branch Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024370010065 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |