CN112438276B - Seed pelleting slow-release coating agent containing metarhizium anisopliae and preparation method thereof - Google Patents
Seed pelleting slow-release coating agent containing metarhizium anisopliae and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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Abstract
The invention relates to a seed pelleting slow-release coating agent containing metarhizium anisopliae and a preparation method thereof, belonging to the technical field of biological pesticides and biological pest control. The seed pelleting slow-release coating agent comprises the following components in percentage by mass: 2-10% of metarhizium anisopliae powder, 5-8% of wetting dispersant, 0.5-2% of antioxidant, 0.2-0.4% of thickening agent, 0.1-0.5% of defoaming agent, 1-2% of film forming agent, 1-2% of plant growth regulator, 30-40% of filling agent and the balance of water. The invention modifies the surface of metarhizium anisopliae spore powder by a physical method, and a layer of film is coated on the outer layer, thereby having the slow release effect after being processed into pellets. The control time of the seed pelleting slow-release coating agent prepared by the invention can reach 20-30 days, and compared with the common microorganism pelleting coating agent, the duration is longer, so that the metarhizium anisopliae can be ensured to continuously and stably play a role, and the control effect on underground pests can be realized during the whole rooting and sprouting period of the seeds.
Description
Technical Field
The invention relates to a seed pelleting slow-release coating agent containing metarhizium anisopliae and a preparation method thereof, belonging to the technical field of biological pesticides and biological pest control.
Background
The entomogenous fungi are important natural enemy factors capable of naturally regulating insect populations, and have the characteristics of rich resources, no harm to people and livestock, no environmental pollution and the like. Metarhizium anisopliae (Metarhizium anisopliae) is an important entomopathogenic fungus, has the infection capacity on coleoptera, lepidoptera, hymenoptera, homoptera, diptera and other insects, and particularly has a good control effect on underground pests (such as grubs).
The coating of the seeds means that substances such as bactericides, insecticides, plant growth regulators and the like are adhered to the surfaces of the seeds by using adhesives. Pelleting is a main seed coating type, the weight of the pelleted and coated seeds is increased, so that the seeds are easy to fix in the soil, the problem that the seeds drift along with wind during aerial sowing can be solved, and meanwhile, nutrients and active substances are added on the surfaces of the seeds through pelleting, so that the seed growth and the resistance to pest and disease infection are facilitated. At present, the seed coating agent with slow release function in the development stage of the whole world is only a microcapsule type suspension seed coating agent, and the seed coating agent is prepared by wrapping active ingredients by polymer pellets with the diameter of 5-20 microns or less to form a microcapsule, and then processing the microcapsule into a water suspension type or dry gel suspension type seed coating agent according to the requirement of the seed coating agent. Therefore, the microcapsule type suspension seed coating agent needs to be processed into the microcapsule suspension agent firstly. The preparation method of the microcapsule suspending agent mainly comprises two methods, namely an in-situ polymerization method and an interface polymerization method. However, if the seed coating agent is prepared by using microorganisms as active ingredients, the active ingredients are live spores, and the spores are inevitably influenced by the ambient temperature and other forces, so that the spore germination rate is influenced. The in-situ polymerization method involves a step of heating and solidifying in the preparation process, the temperature is generally required to be kept at about 60-70 ℃, and even if strains with high temperature resistance are screened, the activity of spores is seriously influenced. However, the interfacial polymerization method is not suitable for this method because the active ingredient having an active group on the molecule cannot be encapsulated because the reactivity of the monomer of the wall material is high and the active group of the active ingredient takes part in the reaction.
The existing technical system for preparing microcapsule type suspension seed coating agent is to form a layer of surface covering film on the particle surface by chemical synthesis, but the surface covering is less by physical method. The invention adopts a physical method to prepare the metarhizium anisopliae pelletized sustained-release coating agent, and solves the problem that the activity of metarhizium anisopliae spores is damaged by chemically synthesizing a microcapsule film.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a novel pelleted slow-release coating agent containing metarhizium anisopliae seeds, which modifies the surface of metarhizium anisopliae spore powder through a physical method, and coats a layer of film on the outer layer, so that the slow-release coating agent has a slow-release effect after being processed into pellets.
The invention also aims to provide a preparation method of the pelleting slow-release coating agent for the seeds containing the metarhizium anisopliae.
In order to achieve the purpose, the invention adopts the technical scheme that: a seed pelleting slow-release coating agent containing metarhizium anisopliae comprises the following components: metarhizium anisopliae powder, a wetting dispersant, an antioxidant, a thickening agent, a defoaming agent, a film-forming agent, a plant growth regulator, a filling agent and water.
The metarhizium anisopliae provided by the invention can be separated from soil and used as a main component of a seed pelleting slow-release coating agent, the compatibility of seeds and soil can be well improved, a beneficial microbial environment is constructed to promote the germination and growth of the seeds, and meanwhile, the screened metarhizium anisopliae strain is resistant to high temperature and ultraviolet rays, so that the preparation of a seed coating agent is facilitated.
Preferably, the seed pelleting slow-release coating agent comprises the following components in percentage by mass: 2-10% of metarhizium anisopliae powder, 5-8% of wetting dispersant, 0.5-2% of antioxidant, 0.2-0.4% of thickening agent, 0.1-0.5% of defoaming agent, 1-2% of film forming agent, 1-2% of plant growth regulator, 30-40% of filling agent and the balance of water.
Preferably, the wetting dispersant is submicron macromolecular porous polycarboxylate; the particle size of the submicron order polymer porous polycarboxylate is 0.1-1 μm, and the molecular weight of the submicron order polymer porous polycarboxylate is 2000-3000. More preferably, the submicron order polymeric porous polycarboxylate is at least one selected from G-102 and G-103.
Preferably, the antioxidant is at least one selected from the group consisting of 2, 6-di-tert-butyl-4-methylphenol (BHT), Butyl Hydroxyanisole (BHA), and tert-butylhydroquinone (TBHQ).
Preferably, the thickener is a polyacrylic thickener;
preferably, the defoaming agent is selected from silicones, C8-C10At least one of fatty alcohol and polyether defoaming agent; the film forming agent is selected from at least one of polyvinyl alcohol, polyoxyethylene, butyl cellulose and hydroxypropyl cellulose.
Preferably, the plant growth regulator is selected from at least one of gibberellin, indoleacetic acid, ABT rooting powder and naphthylacetic acid.
Preferably, the filler is selected from at least one of bentonite, montmorillonite, sepiolite and diatomite.
Preferably, the seed pelleting slow-release coating agent comprises the following components in percentage by mass: 5% of metarhizium anisopliae powder, G-1037%, 1.3% of tert-butyl hydroquinone, 0.25% of polyacrylic acid thickening agent, 0.3% of organic silicone, 1% of butyl cellulose, 1% of gibberellin, 30% of bentonite and the balance of water.
In another aspect, the present invention provides a preparation method of the pelleted slow-release coating agent for seeds containing metarhizium anisopliae, comprising the following steps:
(1) putting metarhizium anisopliae powder, a wetting dispersant, an antioxidant, a thickening agent, a defoaming agent, a film forming agent, a plant growth regulator and water into a shearing reaction kettle, uniformly shearing, and then performing wet sanding by a sand mill to prepare a suspension;
(2) and (2) putting seeds into a horizontal roller by using a rotary seed pelleting machine by adopting a rolling granulation method, adding the suspension and the filler in the step (1) when the seeds roll, and drying after pelleting to obtain the seed pelleting slow-release coating agent.
Preferably, in the step (1), the time for wet sanding by the sand mill is 1-2 h.
The invention provides a preparation method of a pelleted slow-release coating agent containing metarhizium anisopliae seeds. Modifying the surfaces of metarhizium anisopliae particles by using a submicron-grade high-molecular porous polymer, reducing the surface tension of the particles and simultaneously forming steric hindrance and electrostatic steric hindrance, thereby physically forming a thicker surface covering film; and the polyacrylic acid thickener synthesized by the micro-crosslinked high molecular polymer has better fluidity and elasticity, and can have synergistic action with the submicron high molecular porous polymer to form secondary coating. The thickness of the surface film after the secondary coating is about 300-400 nm through a particle size distribution test.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention adopts a physical method to prepare the pelleted slow-release coating agent containing the metarhizium anisopliae seeds, and solves the problem that the spore activity of the metarhizium anisopliae is damaged by chemically synthesizing a microcapsule film. Meanwhile, compared with the microcapsule suspending agent, the method for preparing the suspending liquid with the slow release performance has simpler processing technology and shorter processing time;
(2) the control time of the seed pelleting slow-release coating agent prepared by the invention can reach 20-30 days, and compared with the common microorganism pelleting coating agent, the duration is longer, so that the metarhizium anisopliae can be ensured to continuously and stably play a role, and the control effect on underground pests can be realized during the whole rooting and sprouting period of the seeds.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1: seed pelleting slow-release coating agent containing 2% metarhizium anisopliae
The preparation method of the seed pelleting slow-release coating agent containing 2% metarhizium anisopliae comprises the following steps:
(1) putting the metarhizium anisopliae powder, G-102, BHA, polyacrylic acid thickening agent, polyether defoaming agent, polyvinyl alcohol, heteroauxin, naphthylacetic acid and water into a shearing reaction kettle according to the proportion, and uniformly shearing at low speed; then sanding for 2 hours by a sand mill wet method to prepare suspension;
(2) and (2) putting the seeds into a horizontal roller by using a rotary seed pelleting machine by adopting a rolling granulation method, adding the suspension and the diatomite in the step (1) when the seeds roll, and drying after pelleting to obtain the seed pelleting slow-release coating agent.
Example 2: seed pelleting slow-release coating agent containing 5% metarhizium anisopliae
The preparation method of the seed pelleting slow-release coating agent containing 5% metarhizium anisopliae comprises the following steps:
(1) putting the metarhizium anisopliae powder, G-103, TBHQ, polyacrylic acid thickening agent, organic silicone, butyl cellulose, gibberellin and water into a shearing reaction kettle according to the proportion, uniformly shearing at low speed, and performing wet sanding for 2 hours by a sand mill to prepare suspension;
(2) and (2) putting seeds into a horizontal roller by using a rotary seed pelleting machine by adopting a rolling granulation method, adding the suspension and bentonite in the step (1) when the seeds roll, and drying after pelleting to obtain the seed pelleting slow-release coating agent.
Example 3: seed pelleting slow-release coating agent containing 10% metarhizium anisopliae
The preparation method of the pelleted slow-release coating agent containing 10% metarhizium anisopliae comprises the following steps:
(1) firstly, putting the metarhizium anisopliae powder, G-102, BHT, polyacrylic acid thickening agent, C8-C10 fatty alcohol, polyvinyl alcohol, gibberellin, NAA and water into a shearing reaction kettle according to the proportion, and uniformly shearing at a low speed; then sanding for 2 hours by a sand mill wet method to prepare suspension;
(2) and (2) putting seeds into a horizontal roller by using a rotary seed pelleting machine by adopting a rolling granulation method, adding the suspension and bentonite in the step (1) when the seeds roll, and drying after pelleting to obtain the seed pelleting slow-release coating agent.
Example 4: summary of test data applied to preventing and treating grubs of cruciferous crops
This example is to verify the effect of the granulated slow-release coating agent containing metarhizium anisopliae seeds of examples 1-3 of the present invention applied to cruciferous crops for controlling grubs, and a drug effect test was performed. The test crop is rape, the control object is grub, the seed pelleting slow-release coating agent of the embodiment 1-3 and rape seeds are prepared into metarhizium anisopliae pelleting slow-release coating agent which is marked as a first test group, a second test group and a third test group respectively; the submicron order polymer porous polycarboxylate and the polyacrylic acid thickener in the examples 1-3 are partially replaced by common wetting dispersant and thickener with the same mass, other components and the preparation method are not changed, and the obtained pelleting coating agents are respectively marked as CK1, CK2 and CK3 to be used as comparison groups; rape seed CK without any treatment was blank.
In a vegetable field with severe grubs, the 3 test groups, the 3 control groups and the blank control are respectively treated by 7 treatments and field sowing is carried out in 7 test cells with equal areas, each cell is equally divided into 10 rows, 10 seeds are sowed in each row, each treatment is repeated for 3 times, the number of seedlings of each treatment after 7 days of sowing is investigated, and the rate of emergence is calculated, and the results are shown in table 1.
TABLE 1 emergence rates of oilseed rape seeds treated differently
As can be seen from Table 1, the emergence rates of the rape seeds (examples 1-3) coated by the metarhizium anisopliae seed pelleting are all up to 86% or more, which indicates that the metarhizium anisopliae pelleting coating can effectively protect rape seedlings from grubs.
Selecting 7 cultivation pots with the same specification, adding soil with the same weight, and putting 10 grubs with basically identical sizes into each cultivation pot. After sowing in the field for 7d, selecting 30 rape seedlings with basically consistent growth vigor for each treatment, uniformly planting the rape seedlings in a cultivation pot in connection with soil, placing the rape seedlings in an indoor laboratory with the constant temperature of 25 ℃, the humidity of 80 +/-5%, the illumination of 16h and the darkness of 8h for cultivation, repeating each treatment for three times, investigating the number of insect pest plants of each treated rape seedling after 5d, 10d and 20d, calculating the insect pest rate and the control effect after 20d, and obtaining the results shown in table 2. Insect pest rate (%) (number of insect pest plants/total number of plants) × 100%; the control effect (%) (blank group pest ratio-control group pest ratio)/blank group pest ratio.
TABLE 2 control of grubs by different treatments
As can be seen from Table 2, the number of the pest plants of the rape seedlings in the white group at 5d is large, and the number of the pest plants in the comparison group and the test group is small; thus, the general metarhizium anisopliae pelletized sustained-release coating agent still has the control effect. After 10 days, the number of insect pest plants in a comparison group is increased, the insect pest rate is above 40% after 20 days, and the control effect is below 40%; the test group shows that the number of the insect pests is still small after 20 days, the insect pest rate is about 12 percent after 20 days, and the control effect is over 80 percent, which shows that the metarhizium anisopliae pill slow-release coating agent has slow-release effect and has the lasting period of about 27 days for the control of the white grubs.
Example 5: summary of test data applied to cruciferous crops for preventing and treating flea beetles
The experiment is to explore the effect of the seed pelleting slow-release coating agent containing the metarhizium anisopliae in the embodiments 1-3 of the invention applied to cruciferous crops for preventing and treating flea beetles, and a pesticide effect experiment is carried out. The test crop was flowering cabbage, the control target was phyllotreta striolata, and the seed treatment of the flowering cabbage was as in example 4.
In alternate phyllotreta striolata generations, perennial field, the treated flowering cabbage seeds were sown as in example 4. After 25 days, 10 cabbage seedlings with basically consistent growth vigor are selected for each treatment, the roots of the cabbage seedlings are moisturized by using wet paper towels and placed in a transparent test tube with a plug, 10 heads of phyllotreta striolata adults are inserted into each tube, and a rubber plug is plugged. Culturing in laboratory at constant temperature of 25 deg.C and humidity of 80 + -5%, lighting for 16h and darkness for 8h, repeating each treatment for three times, investigating death number of flea beetle adults after 72h, and calculating the preventing and treating effect, the results are shown in Table 3.
TABLE 3 control of Phyllotreta striolata by different treatments
As can be seen from the table 3, the general metarhizium anisopliae pelletized slow-release coating agent has almost no control effect after 25 days, but the control effect of the invention on the phyllotreta striolata after 25 days is still more than 83%, which indicates that the seed pelletized slow-release coating agent containing the metarhizium anisopliae provided by the invention has a slow-release effect and long lasting period on the phyllotreta striolata.
Example 6: comparison of Metarhizium anisopliae spore activity
Respectively preparing suspension liquid according to the step (1) in the preparation method provided by the invention in the embodiments 1, 2 and 3, and marking the suspension liquid as (I), (II) and (III); examples 1, 2 and 3 metarhizium anisopliae powder, a film forming agent, an antioxidant and a plant growth regulator are kept unchanged, a capsule shell and a microcapsule auxiliary agent are added, and the metarhizium anisopliae microcapsule suspension seed coating agent is respectively prepared by an in-situ polymerization method and an interfacial polymerization method and is respectively marked as A1, A2, A3 and B1, B2 and B3. The suspension was diluted so that the number of spores was not more than 100 per 10uL, 10uL was plated on a PDA plate, the number of spores was recorded by observation under a microscope, and the suspension was kept at 28 ℃ and kept moist for 36 hours. And observing the number of fungus germination on the PDA after 36h, calculating the spore germination rate, repeating the steps for three times, and taking an average value. The results are shown in Table 4.
TABLE 4 spore germination Rate results
As can be seen from Table 4, the spore germination rate of the metarhizium anisopliae microcapsule suspension seed coating prepared by the in-situ polymerization method and the interface is only about 38-45%, while the spore germination rate of the metarhizium anisopliae suspension prepared by the invention is more than 96%, so that the activity of the metarhizium anisopliae cannot be damaged in the process of preparing the seed pelleting slow-release coating agent containing the metarhizium anisopliae, and the obvious influence on the activity is not caused.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (1)
1. A preparation method of a seed pelleting slow-release coating agent containing metarhizium anisopliae is characterized by comprising the following steps:
(1) putting metarhizium anisopliae powder, a wetting dispersant, an antioxidant, a thickening agent, a defoaming agent, a film forming agent, a plant growth regulator and water into a shearing reaction kettle, uniformly shearing, and performing wet sanding for 2 hours by a sand mill to prepare a suspension;
(2) putting seeds into a horizontal roller by using a rotary seed pelleting machine by adopting a rolling granulation method, adding the suspension and the filler in the step (1) when the seeds roll, and drying after pelleting to obtain the seed pelleting slow-release coating agent;
in the step (1), the wetting dispersant is submicron macromolecular porous polycarboxylate, and the submicron macromolecular porous polycarboxylate is G-103; the antioxidant is tert-butyl hydroquinone, the thickener is a polyacrylic acid thickener, the defoaming agent is organosilicone, the film-forming agent is butyl cellulose, and the plant growth regulator is gibberellin;
in the step (2), the filler is bentonite;
the seed pelleting slow-release coating agent comprises the following components in percentage by mass: 5% of metarhizium anisopliae powder, G-1037%, 1.3% of tert-butyl hydroquinone, 0.25% of polyacrylic acid thickening agent, 0.3% of organic silicone, 1% of butyl cellulose, 1% of gibberellin, 30% of bentonite and the balance of water.
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| CN105284866A (en) * | 2015-11-05 | 2016-02-03 | 浙江新安化工集团股份有限公司 | Seed treatment agent composition having sustained release and repellent effects |
| CN109757488A (en) * | 2019-02-20 | 2019-05-17 | 广东省农业科学院植物保护研究所 | A kind of microorganism seed pill pelletizing coating composition |
| CN111631231A (en) * | 2020-07-02 | 2020-09-08 | 贵州大学 | Biological medicine-fertilizer composite pelletized seed coating agent pelletized seed and preparation method thereof |
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