CN112414760B - Ring fiber core beak-shaped optical fiber tweezers system with stable capturing function - Google Patents

Ring fiber core beak-shaped optical fiber tweezers system with stable capturing function Download PDF

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CN112414760B
CN112414760B CN202011328629.0A CN202011328629A CN112414760B CN 112414760 B CN112414760 B CN 112414760B CN 202011328629 A CN202011328629 A CN 202011328629A CN 112414760 B CN112414760 B CN 112414760B
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optical fiber
beak
fiber
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tweezers
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CN112414760A (en
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成煜
冯智宇
申泽
苑立波
陈明
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Guilin University of Electronic Technology
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02395—Glass optical fibre with a protective coating, e.g. two layer polymer coating deposited directly on a silica cladding surface during fibre manufacture
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/036—Optical fibres with cladding with or without a coating core or cladding comprising multiple layers
    • G02B6/03605—Highest refractive index not on central axis
    • G02B6/03611—Highest index adjacent to central axis region, e.g. annular core, coaxial ring, centreline depression affecting waveguiding
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/255—Splicing of light guides, e.g. by fusion or bonding
    • G02B6/2552—Splicing of light guides, e.g. by fusion or bonding reshaping or reforming of light guides for coupling using thermal heating, e.g. tapering, forming of a lens on light guide ends

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Abstract

The optical tweezers comprise a fiber core beak-shaped optical fiber probe, a laser, an adjusting frame, a sample groove, a microscopic imaging system, a Raman laser source and a Raman detector. The beak-shaped optical fiber probe is characterized in that the distribution of evanescent fields on the side surface of the optical fiber is enhanced by changing the curvature radius of the bent tip area of the optical fiber, and the gradient force borne by cells is increased, so that the cells are stably captured. And finally introducing Raman laser into the beak-shaped optical tweezers, and obtaining corresponding Raman frequency shift through a detector, thereby realizing the characteristic detection of the cells.

Description

Ring fiber core beak-shaped optical fiber tweezers system with stable capturing function
(I) technical field
The invention relates to a ring fiber core beak-shaped optical fiber optical tweezers system with a stable capturing function, and belongs to the technical field of optical control.
(II) background of the invention
Since the pioneering work of Ashkin et al, optical tweezers have become one of the most important research tools in the field of biological and physical research. Tapered fiber optical tweezers tools (TOFTs) are an important optical manipulation tool, and are widely used for capturing micro-nano particles and manipulating multi-particles, such as patterning of micro-particle tissues and cells. In typical TOFTs, the fiber tip is tapered using manufacturing methods such as polishing, hot-drawing, and chemical etching. In recent years, a great deal of research has been conducted on the performance of tapered fiber optical tweezers to achieve non-contact trapping, dual-particle trapping, particle rotation, and the like.
The particle capture range is one of the important performance indexes of the optical tweezers. Extending the particle capture range has practical applications that facilitate particle capture, and can have a tremendous impact on particle manipulation in biochemistry and biophysics, among others. In the previous TOFTs study, the fiber tapers were all straight tapers: the light field radiates to free space at the tip of the straight fiber cone and is focused to form a small and strong focus, and a strong capturing force is generated. The light field on the side of the right cone is a very weak evanescent wave, which can only generate a negligible force on the particles. Thus, generally, straight-tapered TOFTs can only capture particles in a small region near the tip of the fiber taper.
With the birth of optical fiber and the continuous development of optical fiber technology, the development of optical tweezers of optical fiber has also made great progress. The optical tweezers based on four-core spiral fiber and its making process (patent No. CN106094099A) and the single fiber optical tweezers based on wavelength division multiplexing technology (patent No. CN109254346B) control the capture and movement of particle by regulating the emergent light parameters of the tunable light source. A single optical fiber optical tweezers (patent number: CN104698532A) based on elliptical core optical fiber and a single optical fiber optical tweezers (patent number: CN103996423A) with laterally adjustable capture position are adjusted by adjusting LP11The mode light field forms an optical potential well to capture the particle. In addition, the applicant can also carry out particle capture by changing the structure of the optical fiber tweezers, such as the optical fiber tweezers probe with the secondary cone angle (patent number: CN109212667A) prepared by the two-step method, which is obtained by adopting secondary corrosion to the optical fiberThe secondary cone angle controllable in a certain range can improve the capture range and the capture efficiency.
However, the process difficulty and the working efficiency of the preparation process of the optical tweezers cannot meet the requirements at the same time, for example, the single optical tweezers based on the wavelength division multiplexing technology (patent number: CN109254346B) and the single optical tweezers based on the elliptical core fiber (patent number: CN104698532A) are simple in preparation process, but can only realize cell capture and position adjustment in the axial direction or in front of the optical fiber tip, and the application range is limited. And a single fiber optical tweezers (patent number: CN103996423A) with a laterally adjustable capture position and a fiber optical tweezers probe (patent number: CN109212667A) with a secondary cone angle, which is prepared by a two-step method, are formed by adopting chemical corrosion, and the concentration ratio is difficult to control, so that the degree of the cone angle is difficult to control, and errors are very likely to occur.
The invention provides a ring fiber core beak-shaped optical fiber optical tweezers system with a stable capturing function, which is an effective method for enhancing a side evanescent field of an optical fiber. The invention adopts a special process to change the curvature radius of the bending cone, can enhance the evanescent field distribution of the side surface of the optical fiber and realizes the stable capture of multiple cells on the side surface of the optical fiber. Compared with the prior art, the beak-shaped optical tweezers can enlarge the cell capture range and improve the capture efficiency, and can be combined with the cell Raman spectrum detection technology to detect the cell characteristics, thereby being beneficial to the progress of scientific research and the medical field.
Disclosure of the invention
The invention provides a ring fiber core beak-shaped optical fiber optical tweezers system with a stable capturing function, which comprises a beak-shaped optical fiber probe, a 940nm laser, a three-dimensional adjusting frame, a 'convex' sample groove, a Raman laser source, a Raman detector and a microscopic imaging system comprising an inverted microscope.
The invention aims to provide a ring fiber core beak-shaped optical fiber tweezers system with a stable capturing function, and the optical fiber tweezers can be used for stably capturing multiple cells.
The invention provides a ring fiber core beak-shaped optical fiber tweezers system with a stable capturing function, wherein the optical fiber tweezers are optical fiber probes with beak-shaped front ends.
Compared with a straight cone, the ring fiber core beak-shaped optical fiber optical tweezers system with the stable capturing function can improve the capturing efficiency of cells, namely, the magnitude of optical potential well force borne by the cells under the light source power of every 1W at the incident boundary is increased, namely, the capturing efficiency represents the magnitude of optical trapping force, so that the capturing efficiency is too low to realize cell capturing.
The invention provides a beak-shaped optical fiber tweezers system with a ring fiber core and a stable capturing function, wherein the optical fiber consists of a coating layer, an outer cladding layer, the ring fiber core and an inner silicon dioxide layer. Wherein, the radius of the coating layer is 150 μm, the radius of the outer cladding layer is 62.5 μm, the radius of the outer core is 47 μm, the thickness of the ring of the core is 5.2 μm, the radius of the inner silica layer is 41.8 μm, the refractive index of the outer cladding layer is 1.457, the refractive index of the core is 1.460, and the refractive index of the inner silica layer is 1.457.
The invention provides a beak-shaped optical fiber probe, wherein the curvature radius of the beak-shaped optical fiber probe represents the falling degree of a bent cone tip relative to the center of a straight cone, and the larger the falling degree is, the smaller the curvature radius is. The curvature radius of the optical fiber is between 200 and 400 mu m.
The preparation method of the beak-shaped optical fiber probe provided by the invention comprises the following specific steps:
firstly, removing a coating layer of the ring fiber core optical fiber, and then cleaning the coating layer by using ethanol to obtain the bare optical fiber. The bare fiber was then placed in a non-uniform hot zone (tungsten filament heating, electrode heating, CO)2Laser heating, H2/O2Heating), non-affine deformation is carried out on the steel, the deformation of the hot end is large, and the deformation of the cold end is small. The ring-shaped fiber core with non-uniform deformation can form a larger evanescent field on the tensile stress surface more easily to form a bird beak-shaped characteristic light field, and the evanescent field is asymmetrically distributed on one side of the hot area.
The invention adopts a ring fiber core beak-shaped optical fiber optical tweezers system to perform stable capture and characteristic detection of multiple cells by changing the curvature radius of a bending cone, and the specific steps are as follows:
firstly, 940nm laser is introduced into the optical fiber optical tweezers, and due to the bending property of the optical fiber tip, light beams are continuously refracted and converged at the bent end, evanescent field distribution of the side surface of the optical fiber optical tweezers is enhanced, a gradient optical field is increased, and gradient force borne by cells is increased along with the increase of the evanescent field distribution. Because the gradient force of the cell is larger than the scattering force, the light trapping force of the cell stably binds the cell on the side surface of the optical fiber. And then moving the beak-shaped optical tweezers to a buffer area, coupling Raman pump laser to the beak-shaped optical tweezers, and finally obtaining corresponding Raman frequency shift on a Raman detector to realize the cell detection function. In addition, as the evanescent field at a plurality of positions on the side surface of the optical tweezers of the optical fiber is enhanced, stable capture and characteristic detection of cells can be carried out at a plurality of positions on the side surface of the optical tweezers of the optical fiber.
The optical tweezers with the ring fiber core and the beak-shaped optical fibers adopt eccentric fusion, namely, a light source is injected by a space method. The ring fiber core beak-shaped optical fiber probe adopts a mode conversion coupler to change an LP01 Gaussian spot into LP21, collimation coupling is carried out through a free space, 940nm laser is coupled into the ring fiber core, and the ring fiber core optical fiber transmits the laser to the beak-shaped optical fiber probe.
The invention has at least the following unique and significant advantages:
(1) the ring fiber core beak-shaped optical fiber tweezers system with the stable trapping function provided by the invention can control the position and the number of cell trapping by controlling the curvature radius of the bending cone, and can provide a wider application range for cell trapping.
(2) Compared with other proposed optical tweezers with the same functions, the optical tweezers with the ring fiber core and the bird beak-shaped optical fiber with the stable trapping function, provided by the invention, can expand the trapping range of cells, and can not only trap the cells at the tip of the cone-shaped optical fiber, but also trap the cells at the side surface of the optical tweezers.
(3) Compared with single fiber core beak-shaped optical fiber tweezers, the ring fiber core beak-shaped optical fiber tweezers system with the stable capturing function provided by the invention not only can capture more cells on the side surface of the optical tweezers, but also can capture cells with larger particle size.
(IV) description of the drawings
FIG. 1 is a schematic view of a beak-shaped optical fiber probe.
FIG. 2 is a schematic cross-sectional view of a ring core.
FIG. 3 is a diagram of an apparatus for manufacturing a bird's beak-shaped optical fiber probe.
FIG. 4 is a schematic diagram of a bird beak fiber probe prepared by non-uniform thermal field.
FIG. 5 is a schematic diagram of a ring core bird's beak type optical fiber tweezers system with stable trapping function.
FIG. 6 is a schematic diagram of a stable cell trapping and characteristic detection experiment using a ring-core beak-shaped optical fiber tweezers system with a stable trapping function.
Fig. 7 is a graph showing the trapping efficiency for different particle sizes at different locations on a beak optical tweezers with a bending radius of 150 μm.
(V) concrete embodiment
The invention provides a ring fiber core beak-shaped optical fiber tweezers system with a stable capturing function, wherein the front end of the optical fiber tweezers is provided with an optical fiber probe with a beak-shaped structure.
As shown in FIG. 1, the optical fiber probe has a tail length of 18 μm and a taper length of 35 μm, and can form bird beak shapes with different bending degrees according to different processes, wherein the upper side is in a parabola-like shape, and the lower side is in an asymmetric structure with a gentle arc line.
As shown in fig. 2, the ring core is composed of a cladding layer, an outer cladding layer, a ring core, and an inner silica layer. Wherein the radius of the coating layer is 150 μm, the radius of the outer cladding layer is 62.5 μm, the outer radius of the fiber core is 47 μm, the thickness of the ring of the fiber core is 5.2 μm, the radius of the inner silica layer is 41.8 μm, the refractive index of the outer cladding layer is 1.457, the refractive index of the fiber core is 1.460, and the refractive index of the inner silica layer is 1.457.
The invention provides a ring fiber core beak-shaped optical fiber optical tweezers system with a stable capturing function, and as shown in fig. 3, a preparation device of a beak-shaped optical fiber probe consists of a weight 1, a ring fiber core optical fiber 2, a non-uniform thermal field device 3 and a control console 4.
As shown in fig. 4, the method for preparing the beak-shaped optical fiber probe includes the following steps:
firstly, taking a ring fiber core optical fiber, removing a coating layer of the ring fiber core optical fiber, and cleaning the ring fiber core optical fiber by using ethanol to obtain a bare optical fiber. Then, the weight 1 was attached to both ends of the treated optical fiber, and the optical fiber was placed on the console 4 to be straightened. Finally, it is placed in the non-uniform thermal field area (tungsten filament heating, electrode heating, CO) generated by the non-uniform thermal field device 32Laser heating, H2/O2Heating), non-affine deformation is performed on the fiber. Because the temperature of the upper end is higher, the deformation degree of the upper end of the optical fiber is larger than that of the lower end, and the annular fiber core with non-uniform deformation can form a larger evanescent field on the tensile stress surface more easily to form a beak-shaped characteristic light field. In addition, a transition region is formed due to the temperature difference between the fiber and the non-uniform thermal field. And at the junction of the transition region and the non-uniform thermal field, a uniform lumbar cone region is formed because the temperature difference between the two regions is not large. At the moment, a bird beak-shaped structure can be formed by bending the tip region, and the evanescent field of the bird beak-shaped structure is non-uniformly and symmetrically distributed on one side of the thermal field.
As shown in fig. 5 and fig. 6, the optical fiber tweezers system of the present invention comprises a ring core beak-shaped optical fiber probe 11, a 940nm laser 5, a three-dimensional adjusting frame 6, a sample groove 7 shaped like a Chinese character 'tu', a raman laser source 8, a raman detector 9, and a microscopic imaging system 10 including an inverted microscope.
As shown in FIG. 7, the present invention uses a bird beak-shaped fiber optic probe with a curvature radius r of 150 μm for stable capture of cells of different particle sizes. The tip of the beak-shaped optical fiber probe was labeled with X15 μm, 20 μm, 25 μm, 30 μm, and 35 μm as Yb1、Yb2、Yb3、Yb4、Yb5。
The ring core beak optical fiber tweezers system with stable trapping function according to the present invention will be further described in detail with reference to the following embodiments, and the technical solutions of the present invention include, but are not limited to, the following embodiments.
Example 1 bird beak-shaped fiber Probe Yb1Capture of cells with different particle sizes
In the system, a steering ringLaser with the wavelength of 940nm is introduced into the fiber core beak-shaped optical fiber, the laser is adjusted by the three-dimensional adjusting frame in the transmission process, the beak-shaped optical fiber probe is placed in the sample groove, the sample groove is observed through an inverted microscope below the beak-shaped optical fiber probe, and finally the beak-shaped optical fiber probe is displayed on a PC through a microscopic imaging system. As shown in FIG. 7, at Yb1In the above case, since the gradient force exerted on the cell having a particle size of 4 μm is smaller than the scattering force exerted on the cell, the resultant force of the gradient force and the scattering force is directed in the direction of light propagation, and therefore the cell cannot be trapped at the position, and the trapping efficiency ξ is 14.73 PN/W. Similarly, cells with particle sizes of 5 μm and 6 μm could not be trapped there, and trapping efficiencies ξ were 17.07PN/W and 19.39 PN/W.
Example 2 bird beak-shaped fiber Probe Yb2Capture of cells with different particle sizes
As shown in FIG. 7, at Yb2The gradient force of the cells with the particle sizes of 4 μm, 5 μm and 6 μm is smaller than the scattering force, the resultant force of the two points to the direction of light propagation, so that the cells cannot be captured at the position, and the capturing efficiencies ξ are 42.47PN/W, 49.41PN/W and 56.05PN/W respectively.
EXAMPLE 3 bird beak-shaped fiber Probe Yb3To capture cells with different particle sizes
As shown in FIG. 7, at Yb3The gradient force of the cell with the particle size of 4 μm is larger than the scattering force, the resultant force of the two forces points to the position where the light field is focused, so the cell can be captured at the position, and the capturing efficiency ξ is 104.60 PN/W. Similarly, cells with particle sizes of 5 μm and 6 μm can be captured at the same place, and the capturing efficiencies ξ are 122.44PN/W and 139.31PN/W, respectively.
Example 4 bird beak-shaped fiber Probe Yb4Capture of cells with different particle sizes
As shown in FIG. 7, at Yb4The gradient force of the cell with the particle sizes of 4 microns, 5 microns and 6 microns is larger than the scattering force of the cell, the resultant force of the gradient force and the scattering force points to the position where the light field is focused, so that the cell can be captured at the position, and the capturing efficiencies xi are 170.12PN/W, 195.70PN/W and 220.00PN/W respectively.
Example 5 bird's beak-shaped optical fiber Probe Yb5Capture of cells with different particle sizes
As shown in FIG. 7, at Yb5The gradient force of the cells with the particle sizes of 4 microns, 5 microns and 6 microns is smaller than the scattering force of the cells, the resultant force of the two is directed to the direction of light propagation, so that the cells cannot be captured at the position, and the capturing efficiency xi is 73.16PN/W, 64.04PN/W and 64.04PN/W respectively.

Claims (6)

1. A ring fiber core beak-shaped optical fiber tweezers system with a stable capturing function is characterized in that the optical fiber tweezers comprise a beak-shaped optical fiber probe, a laser, an adjusting bracket, a sample groove, a Raman laser source, a Raman detector and a microscopic imaging system;
the bird beak-shaped optical fiber probe is in a parabola-like shape at the upper side and is in an asymmetric structure with a gentle arc line at the lower side; the optical fiber tweezers system is used for stably capturing cells and detecting characteristics of the cells and comprises the following steps: firstly, 940nm laser is introduced into the optical fiber optical tweezers, and due to the bending property of the tip end of the optical fiber, light beams are continuously refracted and converged at the bent end part, evanescent field distribution of the side surface of the optical fiber optical tweezers is enhanced, a gradient optical field is increased, and gradient force borne by cells is increased along with the increase of the evanescent field distribution; because the gradient force borne by the cell is greater than the scattering force borne by the cell, the cell is stably bound on the side surface of the optical fiber by the optical trapping force borne by the cell; and then moving the beak-shaped optical tweezers to a buffer area, coupling Raman pump laser to the beak-shaped optical tweezers, and finally obtaining corresponding Raman frequency shift on a Raman detector to realize the cell detection function.
2. The fiber optical tweezers system of claim 1, wherein the optical fiber is comprised of a coating layer, an outer cladding layer, an annular core, and an inner silica layer.
3. The fiber optical tweezers system of claim 1, wherein the radius of curvature of the curved cone of the beak fiber optic probe is characterized by the degree of drop of the curved cone tip of the beak fiber optic probe from the center of the straight cone, and the radius of curvature of the optical fiber is between 200 μm and 400 μm.
4. The fiber optical tweezers system of claim 3, wherein the beak shaped fiber optic probe is prepared by a method comprising the steps of:
firstly, removing a coating layer of the ring fiber core optical fiber, and then cleaning the coating layer with ethanol to obtain a bare optical fiber; then placing the bare optical fiber in a non-uniform hot zone, and carrying out non-affine deformation on the bare optical fiber; the ring-shaped fiber core with non-uniform deformation can form a larger evanescent field on the tensile stress surface more easily to form a bird beak-shaped characteristic light field, and the evanescent field is asymmetrically distributed on one side of the hot area.
5. The fiber optical tweezers system of claim 1, wherein the stable cell capture and characteristic detection using the fiber optical tweezers system comprises the following steps:
firstly, 940nm laser is introduced into the optical fiber, and due to the existence of the bending cone of the beak-shaped optical fiber probe, the light can be refracted and converged continuously at the tip end of the optical fiber, so that evanescent field distribution of the side surface of the optical fiber tweezers is enhanced, and a differential gradient optical field is formed; on the side surface of the bending cone of the beak-shaped optical fiber probe, the direction of the light trapping force borne by the cell is the resultant force direction of the gradient force and the scattering force, and if the gradient force borne by the cell is greater than the scattering force borne by the cell, the resultant force of the gradient force and the scattering force always points to the focusing position of the light field, so that the cell is stably bound on the side surface of the optical fiber, and the stable capture of the cell is realized; and then coupling Raman pump laser to the beak-shaped optical fiber tweezers, and obtaining corresponding Raman frequency shift through a detector, thereby realizing the function of cell characteristic detection.
6. The fiber optical tweezers system of claim 5, wherein the ring core bird's beak fiber optic probe uses a mode conversion coupler to change the LP01 Gaussian spot to LP21, and then couples the collimated light through free space to couple 940nm laser into the ring core, which delivers the laser to the bird's beak fiber optic probe.
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