CN112414506A - Reagent suction detection device and computer readable storage medium - Google Patents
Reagent suction detection device and computer readable storage medium Download PDFInfo
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- CN112414506A CN112414506A CN202011252320.8A CN202011252320A CN112414506A CN 112414506 A CN112414506 A CN 112414506A CN 202011252320 A CN202011252320 A CN 202011252320A CN 112414506 A CN112414506 A CN 112414506A
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- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
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Abstract
The application relates to a reagent air suction detection method and a reagent air suction detection device. And then continuously updating the comparison reference value in the liquid taking process, and judging whether the height of the liquid suction needle can suck enough liquid, and detecting and avoiding reagent air suction through the steps.
Description
Technical Field
The application belongs to the technical field of medical instruments, and particularly relates to a reagent air suction detection device and a computer readable storage medium.
Background
Chemiluminescent instrument need absorb reagent and magnetic bead and participate in the reaction in project test procedure, and reagent and magnetic bead all set up on a circular shape reagent dish usually, and reagent dish is reagent cup accommodation hole all around, and the reagent cup is placed in accommodation hole back, stretches into the reagent cup by the imbibition needle and carries out the imbibition in order to absorb reagent and magnetic bead, detects through the liquid level when absorbing reagent and magnetic bead and judges whether the needle point touches the liquid level. However, in a large number of testing processes, due to the fact that a liquid level detection feedback system is sporadically unstable, a liquid level signal is triggered easily when a needle tip does not touch a liquid level, subsequent liquid sucking actions are caused to suck reagents in an air mode, no effective reagent is added in corresponding project tests, an empty cup value is caused, and result errors are caused. At present, the air suction detection in the market is mainly judged by a pressure sensor, but the method is limited by the signal acquisition precision and stability of the sensor, and phenomena such as false alarm and the like are easy to occur.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: in order to solve the defects in the prior art, the reagent air-aspiration detection device and the computer readable storage medium are provided.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a reagent suction detection method comprises the following steps:
carrying out liquid level detection twice on the reagent bottle to obtain detection times, and if the detection times are not empty, obtaining a liquid level height value;
if the number of times of sounding is 0, comparing liquid level height values obtained by two liquid level detections, judging whether the difference value of the liquid level height values obtained by the two liquid level detections exceeds a set first threshold value, and if the difference value of the liquid level height values obtained by the two liquid level detections does not exceed the set threshold value, designating the hole site as a non-abnormal hole site;
if the number of times of sounding is 1, or if the difference value of the liquid level height values obtained by liquid level detection for two times when the number of times of sounding is 0 exceeds a set first threshold value, performing liquid level detection again on the reagent bottle to judge whether sounding is performed, and if not, acquiring the liquid level height value;
when one of the following conditions is met, the reagent bottle is marked as an abnormal bottle body, and the initial value of the liquid level height is not recorded, otherwise, the reagent bottle is marked as a normal bottle body:
a, detecting the empty times for two times of liquid level detection for 2 times;
b, detecting the liquid level again to obtain a detection result;
c, the difference values of the acquired liquid level height value of the liquid level detection and the liquid level height values of the previous two times exceed a set first threshold value;
d, the number of times of sounding in the two liquid level detections is 1, and the difference value between the liquid level height value obtained by the two liquid level detections and the liquid level height value obtained by the two liquid level detections exceeds a set first threshold value;
if the reagent bottle is a normal bottle body, taking the average of all liquid level height values as a theoretical reference value;
during testing, taking a theoretical reference value as a comparison reference value during first liquid taking, wherein the comparison reference value is A, and taking the liquid level drop caused by subtracting the total liquid absorption amount of N-1 times from A during subsequent liquid taking for the Nth time as the theoretical reference value;
and judging whether the height of the corresponding liquid suction needle during each liquid taking is lower than the liquid level height after the liquid taking, wherein the liquid level height after the liquid taking is the value obtained by subtracting the descending value of the liquid level height after the liquid taking from the comparison reference value during the liquid taking, and if the height of the corresponding liquid suction needle during the liquid taking is higher than the liquid level height after the liquid taking, determining that the liquid taking is invalid and not carrying out the liquid taking.
Preferably, according to the reagent air-suction detection method, when N is larger than or equal to 6, the reference value is obtained by performing linear fitting calculation on the previous reference value N-1 times, and invalid liquid taking is omitted.
Preferably, in the reagent air-aspiration detection method, when N is more than or equal to 6 and the number of times of effective liquid extraction is more than 5, the reference value is obtained by performing linear fitting calculation on the reference value for the first N-1 times.
Preferably, in the reagent aspiration detection method of the present invention, the height of the pipette needle is obtained by recording the stroke of the motor by an encoder on the pipette needle actuation stepping motor.
The invention also provides a reagent suction detection device, which comprises:
the device comprises a liquid level detection module, a comparison reference value determination module and a judgment module;
the liquid level detection module is used for carrying out liquid level detection on the reagent bottle twice to obtain detection sounding times, and if the reagent bottle is not probed, a liquid level height value is obtained; if the number of times of sounding is 0, comparing liquid level height values obtained by two liquid level detections, judging whether the difference value of the liquid level height values obtained by the two liquid level detections exceeds a set first threshold value, and if the difference value of the liquid level height values obtained by the two liquid level detections does not exceed the set threshold value, designating the hole site as a non-abnormal hole site;
if the number of times of sounding is 1, or if the difference value of the liquid level height values obtained by liquid level detection for two times when the number of times of sounding is 0 exceeds a set first threshold value, performing liquid level detection again on the reagent bottle to judge whether sounding is performed, and if not, acquiring the liquid level height value; when one of the following conditions is met, the reagent bottle is marked as an abnormal bottle body, and the initial value of the liquid level height is not recorded, otherwise, the reagent bottle is marked as a normal bottle body:
a, detecting the empty times for two times of liquid level detection for 2 times;
b, detecting the liquid level again to obtain a detection result;
c, the difference values of the acquired liquid level height value of the liquid level detection and the liquid level height values of the previous two times exceed a set first threshold value;
d, the number of times of sounding in the two liquid level detections is 1, and the difference value between the liquid level height value obtained by the two liquid level detections and the liquid level height value obtained by the two liquid level detections exceeds a set first threshold value;
if the reagent bottle is a normal bottle body, taking the average of all liquid level height values as a theoretical reference value;
the comparison reference value determining module is used for taking a theoretical reference value as a comparison reference value during first liquid taking when testing is carried out, wherein the comparison reference value is A, and liquid level reduction caused by subtracting total liquid absorption amount of N-1 times from A during subsequent liquid taking is taken as the theoretical reference value for the Nth liquid taking;
and judging whether the height of the corresponding liquid suction needle during each liquid taking is lower than the liquid level height after the liquid taking, wherein the liquid level height after the liquid taking is the value obtained by subtracting the descending value of the liquid level height after the liquid taking from the comparison reference value during the liquid taking, and if the height of the corresponding liquid suction needle during the liquid taking is higher than the liquid level height after the liquid taking, determining that the liquid taking is invalid and not carrying out the liquid taking.
Preferably, when N is larger than or equal to 6, the reagent air-suction detection device performs linear fitting calculation on the previous N-1 reference values to obtain a reference value, and abandons invalid liquid taking.
Preferably, in the reagent air-aspiration detection device, when N is more than or equal to 6 and the number of times of effective liquid extraction is more than 5, the reference value is obtained by performing linear fitting calculation on the reference value for the first N-1 times.
Preferably, in the reagent aspiration detection device of the present invention, the height of the pipette needle is obtained by recording the stroke of the motor by an encoder on the pipette needle actuation stepping motor.
The invention has the beneficial effects that:
according to the reagent air suction detection method and device, the liquid level height of the reagent bottle can be detected by detecting the effective liquid level twice, and the theoretical reference value is determined by detecting twice. And then continuously updating the comparison reference value in the liquid taking process, and judging whether the height of the liquid suction needle can suck enough liquid, and detecting and avoiding reagent air suction through the steps.
Drawings
The technical solution of the present application is further explained below with reference to the drawings and the embodiments.
FIG. 1 is a flow chart of a reagent aspiration detection method according to an embodiment of the present application;
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
The technical solutions of the present application will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
Example 1
The embodiment provides a reagent aspiration detection method, as shown in fig. 1, including the following steps:
carrying out liquid level detection twice on the reagent bottle to obtain detection times, and if the detection times are not empty, obtaining a liquid level height value;
if the number of times of sounding is 0, comparing liquid level height values obtained by two liquid level detections, and judging whether a difference value of the liquid level height values obtained by the two liquid level detections exceeds a set first threshold (the setting of the first threshold is generally not more than 5%), if the difference value of the liquid level height values obtained by the two liquid level detections does not exceed the set threshold, designating the hole site as a non-abnormal hole site;
if the number of times of sounding is 1, or if the difference value of the liquid level height values obtained by liquid level detection for two times when the number of times of sounding is 0 exceeds a set first threshold value, performing liquid level detection again on the reagent bottle to judge whether sounding is performed, and if not, acquiring the liquid level height value;
when one of the following conditions is met, the reagent bottle is marked as an abnormal bottle body, and the initial value of the liquid level height is not recorded, otherwise, the reagent bottle is marked as a normal bottle body:
a, detecting the empty times for two times of liquid level detection for 2 times;
b, detecting the liquid level again to obtain a detection result;
c, the difference values of the acquired liquid level height value of the liquid level detection and the liquid level height values of the previous two times exceed a set first threshold value;
d, the number of times of sounding in the two liquid level detections is 1, and the difference value between the liquid level height value obtained by the two liquid level detections and the liquid level height value obtained by the two liquid level detections exceeds a set first threshold value;
if the reagent bottle is a normal bottle body, taking the average of all liquid level height values as a theoretical reference value;
during testing, taking a theoretical reference value as a comparison reference value during first liquid taking, wherein the comparison reference value is A, and taking the liquid level drop caused by subtracting the total liquid absorption amount of N-1 times from A during subsequent liquid taking for the Nth time is taken as the comparison reference value; the liquid level drop caused by the amount of liquid aspirated at each pipetting is obtained by dividing the amount of liquid aspirated by the cross-sectional area of the reagent bottle, and the total amount of liquid aspirated is obtained by dividing the sum of all the amounts of liquid aspirated at N-1 pipetting by the cross-sectional area of the reagent bottle.
And judging whether the height of the corresponding liquid suction needle during each liquid taking is lower than the liquid level height after the liquid taking (the liquid level height after the liquid taking is a predicted value, and the liquid suction amount required by the liquid taking is divided by the cross sectional area of the reagent bottle to predict), wherein the liquid level height after the liquid taking is a comparison reference value during the liquid taking minus a descending value of the liquid level height after the liquid taking, and if the height of the corresponding liquid suction needle during the liquid taking is higher than the liquid level height after the liquid taking, determining that the liquid taking is invalid and not performing the liquid taking. If the height of the corresponding liquid suction needle is lower than the liquid level height after the liquid is taken for the time when the liquid is taken, the liquid suction needle is considered to be capable of sucking enough liquid, and therefore the liquid is considered to be effective for the time.
As an embodiment: and when N is more than or equal to 6, performing linear fitting calculation according to the previous N-1 comparison reference values to obtain a comparison reference value of the liquid level height during next liquid taking, and discarding invalid liquid taking.
When N is more than or equal to 6, enough fitting points exist, and the accuracy of fitting data is improved.
As a preferred embodiment: and when N is more than or equal to 6 and the effective liquid taking times are more than 5, performing linear fitting calculation through the previous N-1 comparison reference values to obtain a comparison reference value of the liquid level height in the next liquid taking process.
The height of the liquid suction needle is obtained by recording the stroke of the motor by an encoder on the liquid suction needle action stepping motor.
Example 2
The embodiment provides a reagent aspiration detection device, includes:
the device comprises a liquid level detection module, a comparison reference value determination module and a judgment module;
the liquid level detection module is used for carrying out liquid level detection on the reagent bottle twice to obtain detection sounding times, and if the reagent bottle is not probed, a liquid level height value is obtained; if the number of times of sounding is 0, comparing liquid level height values obtained by two liquid level detections, judging whether the difference value of the liquid level height values obtained by the two liquid level detections exceeds a set first threshold value, and if the difference value of the liquid level height values obtained by the two liquid level detections does not exceed the set threshold value, designating the hole site as a non-abnormal hole site;
if the number of times of sounding is 1, or if the difference value of the liquid level height values obtained by liquid level detection for two times when the number of times of sounding is 0 exceeds a set first threshold value, performing liquid level detection again on the reagent bottle to judge whether sounding is performed, and if not, acquiring the liquid level height value; when one of the following conditions is met, the reagent bottle is marked as an abnormal bottle body, and the initial value of the liquid level height is not recorded, otherwise, the reagent bottle is marked as a normal bottle body:
a, detecting the empty times for two times of liquid level detection for 2 times;
b, detecting the liquid level again to obtain a detection result;
c, the difference values of the acquired liquid level height value of the liquid level detection and the liquid level height values of the previous two times exceed a set first threshold value;
d, the number of times of sounding in the two liquid level detections is 1, and the difference value between the liquid level height value obtained by the two liquid level detections and the liquid level height value obtained by the two liquid level detections exceeds a set first threshold value;
if the reagent bottle is a normal bottle body, taking the average of all liquid level height values as a theoretical reference value;
the comparison reference value determining module is used for taking a theoretical reference value as a comparison reference value during first liquid taking when testing is carried out, wherein the comparison reference value is A, and liquid level reduction caused by subtracting total liquid absorption amount of N-1 times from A during subsequent liquid taking is taken as the comparison reference value for the Nth liquid taking;
and judging whether the height of the corresponding liquid suction needle during each liquid taking is lower than the liquid level height after the liquid taking, wherein the liquid level height after the liquid taking is the value obtained by subtracting the descending value of the liquid level height after the liquid taking from the comparison reference value during the liquid taking, and if the height of the corresponding liquid suction needle during the liquid taking is higher than the liquid level height after the liquid taking, determining that the liquid taking is invalid and not carrying out the liquid taking.
As an embodiment: and when N is more than or equal to 6, performing linear fitting calculation according to the previous N-1 comparison reference values to obtain a comparison reference value of the liquid level height during next liquid taking, and discarding invalid liquid taking.
As a preferred embodiment: and when N is more than or equal to 6 and the effective liquid taking times are more than 5, performing linear fitting calculation through the previous N-1 comparison reference values to obtain a comparison reference value of the liquid level height in the next liquid taking process.
The height of the liquid suction needle is obtained by recording the stroke of the motor by an encoder on the liquid suction needle action stepping motor.
In light of the foregoing description of the preferred embodiments according to the present application, it is to be understood that various changes and modifications may be made without departing from the spirit and scope of the invention. The technical scope of the present application is not limited to the contents of the specification, and must be determined according to the scope of the claims.
As will be appreciated by one skilled in the art, embodiments of the present application may be provided as a method, system, or computer program product. Accordingly, the present application may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present application may take the form of a computer program product embodied on one or more computer-usable storage media having computer-usable program code embodied in the medium.
The present application is described with reference to flowchart illustrations and/or block diagrams of methods, apparatus, and computer program products according to embodiments of the application. It will be understood that each flow and/or block of the flow diagrams and/or block diagrams, and combinations of flows and/or blocks in the flow diagrams and/or block diagrams, can be implemented by computer program instructions. These computer program instructions may be provided to a processor of a general purpose computer, special purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, create means for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
Claims (5)
1. A reagent aspiration detection device, comprising:
the device comprises a liquid level detection module, a comparison reference value determination module and a judgment module;
the liquid level detection module is used for carrying out liquid level detection on the reagent bottle twice to obtain detection sounding times, and if the reagent bottle is not probed, a liquid level height value is obtained; if the number of times of sounding is 0, comparing liquid level height values obtained by two liquid level detections, judging whether the difference value of the liquid level height values obtained by the two liquid level detections exceeds a set first threshold value, and if the difference value of the liquid level height values obtained by the two liquid level detections does not exceed the set threshold value, designating the hole site as a non-abnormal hole site;
if the number of times of sounding is 1, or if the difference value of the liquid level height values obtained by liquid level detection for two times when the number of times of sounding is 0 exceeds a set first threshold value, performing liquid level detection again on the reagent bottle to judge whether sounding is performed, and if not, acquiring the liquid level height value; when one of the following conditions is met, the reagent bottle is marked as an abnormal bottle body, and the initial value of the liquid level height is not recorded, otherwise, the reagent bottle is marked as a normal bottle body:
a, detecting the empty times for two times of liquid level detection for 2 times;
b, detecting the liquid level again to obtain a detection result;
c, the difference values of the acquired liquid level height value of the liquid level detection and the liquid level height values of the previous two times exceed a set first threshold value;
d, the number of times of sounding in the two liquid level detections is 1, and the difference value between the liquid level height value obtained by the two liquid level detections and the liquid level height value obtained by the two liquid level detections exceeds a set first threshold value;
if the reagent bottle is a normal bottle body, taking the average of all liquid level height values as a theoretical reference value;
the comparison reference value determining module is used for taking a theoretical reference value as a comparison reference value during first liquid taking when testing is carried out, wherein the comparison reference value is A, and liquid level reduction caused by subtracting total liquid absorption amount of N-1 times from A during subsequent liquid taking is taken as the comparison reference value for the Nth liquid taking;
and judging whether the height of the corresponding liquid suction needle during each liquid taking is lower than the liquid level height after the liquid taking, wherein the liquid level height after the liquid taking is the value obtained by subtracting the descending value of the liquid level height after the liquid taking from the comparison reference value during the liquid taking, and if the height of the corresponding liquid suction needle during the liquid taking is higher than the liquid level height after the liquid taking, determining that the liquid taking is invalid and not carrying out the liquid taking.
2. The device for detecting reagent aspiration according to claim 1, wherein when N is greater than or equal to 6, a comparison reference value of the liquid level height at the next liquid taking is calculated by linear fitting from the previous comparison reference values N-1 times, and invalid liquid taking is omitted.
3. The device for detecting reagent aspiration according to claim 2, wherein when N is greater than or equal to 6 and the number of times of effective liquid extraction is greater than 5, a comparison reference value of the liquid level height at the next liquid extraction is obtained by performing linear fitting calculation on the comparison reference value for the previous N-1 times.
4. The device for detecting reagent aspiration according to claim 3, wherein the height of the pipette needle is obtained by recording the stroke of a motor by an encoder on the pipette needle action stepping motor.
5. A computer-readable storage medium storing a computer program, wherein the computer program comprises the reagent aspiration detection device according to any one of claims 1 to 4.
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| CN113188840A (en) * | 2021-03-31 | 2021-07-30 | 深圳普门科技股份有限公司 | Sampling method, device, detection equipment and storage medium |
| CN113720417A (en) * | 2021-09-08 | 2021-11-30 | 苏州长光华医生物医学工程有限公司 | Reagent sucking method based on bottle setting |
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