CN112410378A - Uniformly mixed biological fermentation process for microbial technology - Google Patents
Uniformly mixed biological fermentation process for microbial technology Download PDFInfo
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- CN112410378A CN112410378A CN202011351321.8A CN202011351321A CN112410378A CN 112410378 A CN112410378 A CN 112410378A CN 202011351321 A CN202011351321 A CN 202011351321A CN 112410378 A CN112410378 A CN 112410378A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
Abstract
The invention discloses a uniformly mixed biological fermentation process for microbial science and technology, which comprises the following steps: step one, selecting strains; selecting raw materials; step three, seed culture; step four, shake flask culture; step five, stirring for the first time; step six, pressure maintaining and sterilization; seventhly, stirring for the second time; step eight, fermentation culture; step nine, ultrasonic treatment; step ten, filter pressing and drying; the raw materials and the fermentation liquor are respectively stirred, so that the raw materials in the fermentation liquor are favorably and fully mixed, ultrasonic treatment is performed by using the ultrasonic generator in the fermentation process, the metabolism of cells can be increased by ultrasonic waves, the fermentation speed is favorably improved, the uniform mixing degree of microorganisms and the fermentation liquor is further improved by the vibration of the ultrasonic waves, the process is simple, the raw materials are easy to obtain, the waste straws are used as the raw materials, the fermentation cost is reduced, and meanwhile, no chemical additive is added, so that the environment is favorably protected.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a biological fermentation process for microbial technology, which is uniformly mixed.
Background
Fermentation refers to the process by which a person produces the microbial cells themselves, or direct or secondary metabolites, by virtue of the life activities of the microorganism under aerobic or anaerobic conditions.
The biological fermentation engineering is an important component of the biological engineering, microorganisms utilize carbohydrates to ferment and produce various industrial solvents and chemical raw materials, however, the existing biological fermentation process on the market has the condition of uneven mixing of raw materials, the microorganisms and fermentation liquor are not fully mixed in the fermentation process, the fermentation efficiency is reduced, meanwhile, the existing fermentation technology process is complex, various additives are added in the fermentation process, the fermentation cost is high, the production efficiency is low, the economic benefit is reduced, and the addition of the additives can cause certain environmental pollution and is not beneficial to environmental protection.
Disclosure of Invention
The invention aims to provide a biological fermentation process for microbial technology, which is uniformly mixed, so as to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: a uniformly mixed biological fermentation process for microbial technology comprises the following steps: step one, selecting strains; selecting raw materials; step three, seed culture; step four, shake flask culture; step five, stirring for the first time; step six, pressure maintaining and sterilization; seventhly, stirring for the second time; step eight, fermentation culture; step nine, ultrasonic treatment; step ten, filter pressing and drying;
selecting pure yeast, lactic acid bacteria and bacillus spore after screening and culturing as cultured strains in the first step, and freezing and storing the strains;
in the second step, soybean dregs, corn steep liquor, bagasse, straw dregs and sugar residues with low cost are selected as main raw materials of the fermentation liquor;
in the third step, the strain frozen and preserved in the first step is inoculated on a slant for activation, and then the activated strain is inoculated on a flat plate for purification;
in the fourth step, the strain purified in the third step is scraped into a triangular flask sterilized at high temperature by a scraper, a seed culture solution is filled in the triangular flask, the flask is put into a shaking table for shake culture, and a suspension liquid is obtained for later use after the culture is finished;
in the fifth step, the raw materials selected in the second step are put into a crushing stirrer to be crushed and stirred until all the raw materials are fully stirred to prepare fermentation liquor for later use;
in the sixth step, the fermentation liquor prepared in the fifth step is injected into a fermentation tank, a coil pipe of the fermentation tank is opened for steam heating, the fermentation liquor in the fermentation tank is sterilized by high-temperature steam, then high-pressure heat preservation is carried out, and then cooling is carried out to 35-37 ℃ for later use;
in the seventh step, after the fermentation tank in the sixth step is cooled to 35-37 ℃, opening the stirring blades of the fermentation tank, performing secondary stirring on the fermentation liquor, and keeping the fermentation liquor for later use after the stirring is completed;
in the eighth step, the suspension bacterial liquid prepared in the fourth step is poured into the fermentation tank from the inoculation port of the fermentation tank, the pH inside the fermentation tank is adjusted to be 3.4-4.4, and at the moment, the stirring blade of the fermentation tank is opened for constant-temperature fermentation;
in the ninth step, in the fermentation process in the eighth step, carrying out ultrasonic treatment on the fermentation liquor in the fermentation tank by using an ultrasonic generator until the fermentation is finished;
and in the tenth step, conveying the fermentation liquor which is subjected to the ultrasonic treatment in the ninth step and is fermented into a filter press for filter pressing treatment, and obtaining the required fermentation product after the filter pressing treatment.
According to the technical scheme, in the fourth step, the temperature of the shaking table is 35-37 ℃, the rotating speed of the shaking table is 200-.
According to the technical scheme, in the fifth step, the crushing rotating speed of the crushing stirrer is 2500-.
According to the technical scheme, in the sixth step, the temperature of high-temperature steam sterilization is 121-125 ℃, the heat preservation time is 20-30min, and the internal pressure of the fermentation tank is 0.09-0.095 MPa.
According to the technical scheme, in the seventh step, the stirring speed is 150-250 r/min.
According to the technical scheme, in the eighth step, the fermentation process is divided into a front-stage aerobic fermentation stage and a rear-stage anaerobic fermentation stage, the internal temperature of the fermentation tank in the front-stage aerobic fermentation stage is 28-30 ℃, the ventilation amount is 300L/min, the stirring speed is 45-50r/min, the fermentation time is 24-26h, the ventilation is stopped for 10min every 1h, the internal temperature of the fermentation tank in the rear-stage anaerobic fermentation stage is 32-35 ℃, the stirring speed is 40-50r/min, and the fermentation time is 20-24 h.
According to the technical scheme, in the step ten, the working time of the filter press is 10-20min, and the pressure of the filter press is 0.3-0.6 MPa.
Compared with the prior art, the invention has the following beneficial effects: the raw materials and the fermentation liquor are respectively stirred, so that the raw materials in the fermentation liquor are fully mixed and are more uniformly mixed, ultrasonic treatment is performed by using an ultrasonic generator in the fermentation process, the permeability of cell membranes can be increased by ultrasonic waves, the metabolism of cells is increased, the fermentation speed is increased, the uniform mixing degree of microorganisms and the fermentation liquor is further improved by the vibration of the ultrasonic waves, the full reaction of the microorganisms and the fermentation liquor is facilitated, the process is simple and direct, the raw materials are easy to obtain, the fermentation cost is greatly reduced, no chemical auxiliary agent is added, waste straws are used as the raw materials, the waste utilization is realized, and the environment protection is facilitated.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a process flow diagram of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution: a uniformly mixed biological fermentation process for microbial technology comprises the following steps: step one, selecting strains; selecting raw materials; step three, seed culture; step four, shake flask culture; step five, stirring for the first time; step six, pressure maintaining and sterilization; seventhly, stirring for the second time; step eight, fermentation culture; step nine, ultrasonic treatment; step ten, filter pressing and drying;
selecting pure yeast, lactic acid bacteria and bacillus spore after screening and culturing as cultured strains in the first step, and freezing and storing the strains;
in the second step, soybean dregs, corn steep liquor, bagasse, straw dregs and sugar residues with low cost are selected as main raw materials of the fermentation liquor;
in the third step, the strain frozen and preserved in the first step is inoculated on a slant for activation, and then the activated strain is inoculated on a flat plate for purification;
in the fourth step, the strain purified in the third step through one ring is scraped into a triangular flask sterilized at high temperature by a scraper, the triangular flask is filled with seed culture solution, the flask is put into a shaking table for shake culture, the temperature of the shaking table is 35-37 ℃, the rotating speed of the shaking table is 200-250r/min, the culture time is 14-16h, and suspension liquid is obtained for later use after the culture is finished;
in the fifth step, the raw materials selected in the second step are put into a crushing stirrer for crushing and stirring, the crushing speed of the crushing stirrer is 2500-;
injecting the fermentation broth prepared in the fifth step into a fermentation tank, opening a coil pipe of the fermentation tank for steam heating, sterilizing the fermentation broth in the fermentation tank by using high-temperature steam, then performing high-pressure heat preservation, wherein the temperature of the high-temperature steam sterilization is 121-125 ℃, the heat preservation time is 20-30min, the internal pressure of the fermentation tank is 0.09-0.095MPa, and then cooling to 35-37 ℃ for later use;
in the seventh step, after the fermentation tank in the sixth step is cooled to 35-37 ℃, the stirring blade of the fermentation tank is opened, the fermentation liquid is stirred for the second time, the stirring speed is 150-;
pouring the suspension liquid prepared in the fourth step into a fermentation tank from an inoculation port of the fermentation tank, adjusting the pH inside the fermentation tank to be 3.4-4.4, opening a stirring blade of the fermentation tank for constant-temperature fermentation, wherein the fermentation process is divided into an early-stage aerobic fermentation stage and a later-stage anaerobic fermentation stage, the temperature inside the fermentation tank in the early-stage aerobic fermentation stage is 28-30 ℃, the aeration amount is 300L/min, the stirring speed is 45-50r/min, the fermentation time is 24-26h, the fermentation is stopped for 10min every 1h when aeration is carried out, the temperature inside the fermentation tank in the later-stage anaerobic fermentation stage is 32-35 ℃, the stirring speed is 40-50r/min, and the fermentation time is 20-24 h;
in the ninth step, in the fermentation process in the eighth step, carrying out ultrasonic treatment on the fermentation liquor in the fermentation tank by using an ultrasonic generator until the fermentation is finished;
and step ten, conveying the fermentation liquor which is subjected to the ultrasonic treatment in the step nine and is fermented into a filter press for filter pressing treatment, wherein the working time of the filter press is 10-20min, the pressure of the filter press is 0.3-0.6MPa, and the required fermentation product is obtained after the filter pressing is finished.
Based on the above, the method has the advantages that the method is simple in process, convenient to operate and easy to obtain raw materials, waste straws are used as the raw materials, the production cost is greatly reduced, no chemical auxiliary agent is added, the method is beneficial to environmental protection, meanwhile, the fermentation broth is stirred for multiple times in the preparation process, the raw materials are fully mixed, meanwhile, ultrasonic waves are used for processing in the fermentation process, the activity of microorganisms in the fermentation broth is improved, the fermentation efficiency is improved, the microorganisms and the fermentation broth are more uniformly mixed through ultrasonic vibration, the full reaction of the microorganisms and the fermentation broth is facilitated, and the yield of biological products is improved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A uniformly mixed biological fermentation process for microbial technology comprises the following steps: step one, selecting strains; selecting raw materials; step three, seed culture; step four, shake flask culture; step five, stirring for the first time; step six, pressure maintaining and sterilization; seventhly, stirring for the second time; step eight, fermentation culture; step nine, ultrasonic treatment; step ten, filter pressing and drying; the method is characterized in that:
selecting pure yeast, lactic acid bacteria and bacillus spore after screening and culturing as cultured strains in the first step, and freezing and storing the strains;
in the second step, soybean dregs, corn steep liquor, bagasse, straw dregs and sugar residues with low cost are selected as main raw materials of the fermentation liquor;
in the third step, the strain frozen and preserved in the first step is inoculated on a slant for activation, and then the activated strain is inoculated on a flat plate for purification;
in the fourth step, the strain purified in the third step is scraped into a triangular flask sterilized at high temperature by a scraper, a seed culture solution is filled in the triangular flask, the flask is put into a shaking table for shake culture, and a suspension liquid is obtained for later use after the culture is finished;
in the fifth step, the raw materials selected in the second step are put into a crushing stirrer to be crushed and stirred until all the raw materials are fully stirred to prepare fermentation liquor for later use;
in the sixth step, the fermentation liquor prepared in the fifth step is injected into a fermentation tank, a coil pipe of the fermentation tank is opened for steam heating, the fermentation liquor in the fermentation tank is sterilized by high-temperature steam, then high-pressure heat preservation is carried out, and then cooling is carried out to 35-37 ℃ for later use;
in the seventh step, after the fermentation tank in the sixth step is cooled to 35-37 ℃, opening the stirring blades of the fermentation tank, performing secondary stirring on the fermentation liquor, and keeping the fermentation liquor for later use after the stirring is completed;
in the eighth step, the suspension bacterial liquid prepared in the fourth step is poured into the fermentation tank from the inoculation port of the fermentation tank, the pH inside the fermentation tank is adjusted to be 3.4-4.4, and at the moment, the stirring blade of the fermentation tank is opened for constant-temperature fermentation;
in the ninth step, in the fermentation process in the eighth step, carrying out ultrasonic treatment on the fermentation liquor in the fermentation tank by using an ultrasonic generator until the fermentation is finished;
and in the tenth step, conveying the fermentation liquor which is subjected to the ultrasonic treatment in the ninth step and is fermented into a filter press for filter pressing treatment, and obtaining the required fermentation product after the filter pressing treatment.
2. The process of claim 1, wherein the biological fermentation process comprises: in the fourth step, the temperature of the shaking table is 35-37 ℃, the rotation speed of the shaking table is 200-.
3. The process of claim 1, wherein the biological fermentation process comprises: in the fifth step, the crushing rotation speed of the crushing stirrer is 2500-.
4. The process of claim 1, wherein the biological fermentation process comprises: in the sixth step, the temperature of high-temperature steam sterilization is 121-125 ℃, the heat preservation time is 20-30min, and the internal pressure of the fermentation tank is 0.09-0.095 MPa.
5. The process of claim 1, wherein the biological fermentation process comprises: in the seventh step, the stirring speed is 150-250 r/min.
6. The process of claim 1, wherein the biological fermentation process comprises: in the eighth step, the fermentation process is divided into a front-stage aerobic fermentation stage and a rear-stage anaerobic fermentation stage, the internal temperature of the fermentation tank in the front-stage aerobic fermentation stage is 28-30 ℃, the aeration quantity is 300L/min, the stirring speed is 45-50r/min, the fermentation time is 24-26h, the fermentation is stopped for 10min every 1h when aeration is carried out, the internal temperature of the fermentation tank in the rear-stage anaerobic fermentation stage is 32-35 ℃, the stirring speed is 40-50r/min, and the fermentation time is 20-24 h.
7. The process of claim 1, wherein the biological fermentation process comprises: in the step ten, the working time of the filter press is 10-20min, and the pressure of the filter press is 0.3-0.6 MPa.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696423A (en) * | 2009-10-27 | 2010-04-21 | 浙江天台药业有限公司 | Microorganism fermentation process |
| CN107988111A (en) * | 2017-12-26 | 2018-05-04 | 江苏星海生物科技有限公司 | A kind of complex microbial inoculum for handling farm's pollutant and its application |
| CN109704823A (en) * | 2019-03-13 | 2019-05-03 | 黑龙江省农业科学院土壤肥料与环境资源研究所 | Stalk xylo-oligosaccharide content and antioxidant activity are improved using microbial fermented stalk |
| CN110684663A (en) * | 2019-11-27 | 2020-01-14 | 湖南明镜生物科技有限公司 | Preparation process of biological bacteria for pig manure fermentation |
| CN111939100A (en) * | 2020-08-19 | 2020-11-17 | 广州黛诗莎化妆品有限公司 | Water-supplementing hyaluronic acid combined water level fermentation whitening liquid extraction process |
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- 2020-11-26 CN CN202011351321.8A patent/CN112410378A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696423A (en) * | 2009-10-27 | 2010-04-21 | 浙江天台药业有限公司 | Microorganism fermentation process |
| CN107988111A (en) * | 2017-12-26 | 2018-05-04 | 江苏星海生物科技有限公司 | A kind of complex microbial inoculum for handling farm's pollutant and its application |
| CN109704823A (en) * | 2019-03-13 | 2019-05-03 | 黑龙江省农业科学院土壤肥料与环境资源研究所 | Stalk xylo-oligosaccharide content and antioxidant activity are improved using microbial fermented stalk |
| CN110684663A (en) * | 2019-11-27 | 2020-01-14 | 湖南明镜生物科技有限公司 | Preparation process of biological bacteria for pig manure fermentation |
| CN111939100A (en) * | 2020-08-19 | 2020-11-17 | 广州黛诗莎化妆品有限公司 | Water-supplementing hyaluronic acid combined water level fermentation whitening liquid extraction process |
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