CN101696423A - Microorganism fermentation process - Google Patents
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Abstract
The invention relates to a microorganism fermentation process, which comprises two processes including seed culture and fermentation disinfection, wherein the process of the seed culture comprises the following steps of: preparation of a millet inclined plane, a sterile room operation process and seed scraping. The process of the fermentation disinfection comprises the following steps: firstly, blending materials; secondly, putting the prepared strains into a fermentation tank to perform strain culture; thirdly, putting water, washing powder and crushed methoxydienone into a feeding tank, stirring the mixture and pressing the mixture into the fermentation tank; and finally, performing fermentation oxidation, tank releasing and sheet frame filtration. In the feeding process of fermentation culture, a cosolvent is changed into the water and a small amount of the washing powder from an organic solvent, so the microorganism fermentation process not only is environment-friendly but also can reduce the process cost, and can simplify the process steps of liquid waste disposal, wherein the washing powder has a function of a dispersing agent, so the microorganism fermentation process has strong practicability and is suitable for popularized application.
Description
Technical field
The invention belongs to fermentation technique, relate to a kind of microorganism fermentation process.
Background technology
Existing microorganism fermentation process, in the process that feeds intake of fermentation culture, all adopt organic solvent such as propylene glycol etc. as solubility promoter, because organic solvent self price height, and disposing waste liquid also needs many costs also may cause environmental pollution, along with people's environmental consciousness improve constantly and low cost, high efficiency management mode are pursued for obtaining competitive power by enterprise, this traditional microorganism fermentation process will be difficult to continue to use again.
Summary of the invention
The purpose of this invention is to provide a kind of environmental protection, microorganism fermentation process cheaply.
The technical solution adopted in the present invention is to achieve the above object of the invention:
A kind of microorganism fermentation process comprises the steps:
A, cultivation seed, it comprises the steps: again
1. dispose substratum: get millet 0.0001～0.0002%, cleaning the back soaked 25～35 minutes, then millet is cooked, and control pressure is to carry out pressurize sterilization 10～20 minutes under the condition of 100～115 ℃ of 0.04～0.06Mpa, temperature, pulverize in the eggplant bottle of packing into after then the millet that cooks being taken out and carry out disinfection, the sterilization postcooling is during to normal temperature, the millet in the bottle unfolded put tiltedly that to constitute millet seed inclined-plane be substratum (weight percent);
2. carry out sterilisable chamber operation: in sterilisable chamber millet seed inclined-plane is moved to the blank inclined-plane of millet, be placed on after finishing in the permanent room temperature and cultivated 8～12 days, will cultivating bread mould seed after 8～12 days then, to be placed on temperature be to preserve in 0～6 ℃ the refrigerator;
3. scrape kind: the bread mould seed on the seed inclined-plane is scraped in the bottle and break up even one-tenth bacteria suspension with scraper, seal with tampon then, scrape remaining seed inclined-plane with method, above-mentioned bacteria suspension is put into the Erlenmeyer flask of containing sterilized water for per 4～8 bottles synthetic one bottle and is waited for inoculation;
B, fermentation sterilization, it also comprises the steps:
1. batching: by the glucose syrup of total amount 3.5～4%, 3.3～3.6% corn steep liquor, 0.11～0.15% ammonium sulfate, 0.010～0.015% bubble oppose, (Wo Shi thing weight is designated as W for 0.50%～0.85% Wo Shi thing
1), residual content is that the composition of water drops into fermentor tank, then adds to shut cover and open stirring after alkali is transferred PH to 4.2～4.4, then carry out disinfection and inoculate (weight percent);
2. fermentation culture and feeding intake: inoculation earlier, after carry out spawn culture, the temperature of spawn culture is controlled at 26 ± 4 ℃, pressure-controlling is at 0.06 ± 0.10MPa, and is 450～550m by the air flow quantity of incubation time in 1～2h
3/ h, the incubation time air flow quantity in 3～14h is 600～700m
3/ h, incubation time are 700～800m in the air flow quantity of 15h～before feeding intake
3/ h requires the control air flow; Adding weight before beginning to feed intake in charging tank is 3～6W
1Water, add washing powder 0.0024～0.048%, be 0.8～2.0W with weight then
1The Wo Shi thing pulverize the back and drop in the charging tank, open stirring, close cover and the sterilization pipeline that feeds intake, feed liquid can be pressed into fermentor tank (weight percent) when PH descends to changeing when rising;
3. ferment oxidation and put jar: feeding intake enters oxidation period after finishing, behind oxidation 25～35h, begin to do microscopy, seriously should in time put jar if find microbiological contamination, finish sampling oxidation period and detect residual sugar content, begin heating after the sampling, stop after Heating temperature rises to 70～80 ℃ to stir and carry out binder, strengthen tank pressure during binder and guarantee that a feed liquid is seethed in the jar, then feed liquid is pressed into the transfer jar;
4. Plate Filtration: fermented liquid carries out blowing after being pressed into the transfer jar, utilizes tank pressure to carry out press filtration earlier during blowing, when mycelia press dry, dries up with pressurized air, unloading down on the mycelia slave plate, filter cake is ground into fine powder then, has pulverized the back pack.
Described carrying out in the sterilisable chamber operation; the operation steps that millet seed inclined-plane is moved to the blank inclined-plane of millet is: open the seed bottle; insert big bacterial classification of soya bean of seed bottle picking with the culture transferring pin; bacterial classification is put into adds sterilized water in the sterilized water again until watching spore to be as the criterion with naked eyes; make another parallel sample with method; draw the above-mentioned bacterium liquid that makes with suction pipe respectively according to the aseptic technique method then, and move on on the blank millet inclined-plane.
In the described fermentation disinfectant program, the operation steps of sterilization and inoculation is:
A, pressurize sterilization: open steam heating, control steam total pressure is at 0.1～0.25Mpa, and stirring, when treating that a jar temperature reaches 100 ℃, stops stirring, and directly sterilizes with steam, regulates pot liquid and acutely seethes, and makes it even; When tank pressure reaches 0.08Mpa, control steam stagnation pressure; When 118 ℃ of jar Nei Wenduda, begin insulation, 118～120 ℃ of controlled temperature, 20～30 minutes time, pressure-controlling is at 0.09～0.10MPa;
B, pressurize finish postcooling, and when tank pressure was reduced to 0.03～0.05Mpa, logical pressurized air carried out pressurize, inoculate when treating a jar temperature drop to 30 ± 1 ℃;
Whether a temperature in checking before c, the inoculation jar, tank pressure be normal, 26 ± 1 ℃ of inoculation temps, with inoculation mouth on the 75% ethanol wiping jar, close the air inlet valve, when the fast emptying of fermentor tank gas, rapidly pyrosphere is placed on the inoculation mouth, wait jar interior gas to arrange, light pyrosphere, will plant liquid and pour in the fermentor tank from flame hole.
Described Plate Filtration needs to check whether liquid-outlet valve has mycelia to overflow in pressure-filtering process, overflow as finding mycelia, must turn off feed valve at once, and blow for some time with pressurized air, and pressure testing filter again, as not finding to run material, continue again to press, otherwise stop pressure filter work.
The present invention replaces to water and a small amount of washing powder to solubility promoter by organic solvent in the process that feeds intake of fermentation culture, not only environmental protection but also can reduce the technology cost, can also simplify the liquid waste disposal operation, wherein washing powder plays the effect of dispersion agent, so the present invention is practical, is fit to promote the use of.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
Embodiment
A kind of step of microorganism fermentation process is as follows:
1 seed culture process:
1.1 the preparation of substratum (millet inclined-plane):
Claim certain millet quantity, soaked after 30 minutes the cleaning back, millet is put in the pallet paved, and puts in the sterilizing-cabinet to cook, and control pressure is that 0.05Mpa, temperature are 105～110 ℃ during the sterilization pressurize, pressurize 15 minutes; After sterilization finishes, the millet that cooks taken out pulverize, in the eggplant bottle of packing into, the bottled amount of each eggplant is 10g, ties bottleneck and puts in the sterilizing-cabinet sterilization 30 minutes, and pressure is 0.10Mpa, and the sterilization postcooling is unfolded the millet in the bottle during to normal temperature.
1.2 sterilisable chamber operating process:
1.2.1 preparation work: put on the blank inclined-plane in millet seed inclined-plane on the white working suit band, millet after having a bath and use in addition article shift-in sterilisable chamber will cushion outward rapidly between door shut, changing aseptic clothing slippers and band muzzles, advance between second buffering, to use sterilisable chamber in the article dislocation equally, shut the door simultaneously and carry out aseptic technique, earlier clean worktable with 75% ethanol cotton, soak the both hands of oneself, put three flat boards at three places, operator's console left, center, right respectively then, make the sterilisable chamber sterility test.
1.2.2 millet seed inclined-plane moves millet blank inclined-plane operation: right hand thumb; forefinger; middle finger is taken the culture transferring pin; left hand is got the eggplant bottle; right hand little finger; the third finger is opened the tampon on the seed bottle; after the culture transferring pin enters the big bacterial classification of soya bean of seed bottle picking; after tampon got back into the seed bottle; with above-mentioned aseptic technique method bacterial classification is put in the sterilized water of the 100ml left and right sides respectively; in the sterilized water of the 100ml left and right sides, pour in the sterilized water of the 150ml left and right sides again and (can watch spore degree of being) with naked eyes; make another parallel sample with method; draw the above-mentioned bacterium liquid that makes with suction pipe respectively according to the aseptic technique method then; and move on on the blank millet inclined-plane; inclined-plane after culture transferring finishes; bottleneck stopper jam-pack; bind up with gauze; with the method for beaing millet with the abundant mixing of bacterium liquid; be paved into the inclined-plane and put into thermostatic chamber 26 ℃ of following constant temperature culture 9～10 days; write down the primordial seed lot number that culture transferring is used; date; bottle number and exposure experiment colony number; the bread mould seed of cultivating after 9～10 days is loaded on respectively in the dried plastics bag; by last lot number plate, put 2～4 ℃ of preservations of refrigerator.
1.3 scrape kind:
1.3.1 preparation work: the Erlenmeyer flask 12 bottles (1 batches) that scraper, stopper, gauze, kraft paper is bandaged afterwards and fills 800ml water was sterilized 1 hour together, and pressure is 0.15Mpa, and the cooling back is standby; Aseptic clothing and mouth mask sterilization 30 minutes must not surpass two days later behind the production equipment disinfection; Inoculating appliance (except that bacterial classification) was placed the sterilization of sterilisable chamber ultraviolet lamp more than 45 minutes, opened simultaneously and purified working power, ventilated 30 minutes; Put on working suit band seed inclined-plane after operator have a bath and advance sterilisable chamber, the company's of putting on hatband footwear formula aseptic clothing simultaneously is with 75% ethanol erasing worktable and soak both hands.
1.3.2 scrape kind: the sterilized water in the inoculation bottle is poured into respectively in 6 inclined-planes, right hand thumb, forefinger, middle finger take out scraper from Glass tubing, left hand takes inclined-plane, right ring finger, little finger of toe to open tampon, with scraper thallospore is broken up even one-tenth bacteria suspension, tampon is beyond the Great Wall scraped remaining seed inclined-plane with method then, above-mentioned bacteria suspension per 5～6 bottles synthetic a bottle (containing the Erlenmeyer flask of sterilized water), fill source recording behind the operation section end, wait for inoculation.
2 fermentation sterilizations:
2.1 batching:
2.1.1 supplementary material proportioning and charging capacity
| The supplementary material title | Proportioning | Charging capacity | Remarks |
| Glucose syrup | ??3.7％ | ??1400Kg | Nutritive ingredient is provided |
| Corn steep liquor | ??3.45％ | ??1300Kg | Nutritive ingredient is provided |
| Ammonium sulfate | ??0.13％ | ??49Kg | Nutritive ingredient is provided |
| Sodium hydroxide | In right amount | In right amount | Transfer PH |
| The bubble enemy | ??0.012％ | ??4.5Kg | Eliminate foam |
| The Wo Shi thing | ??0.55～0.78％ | ??200～300Kg(W 1) | Substrate |
2.1.2 batching: corn steep liquor is pressed into test tank, to 1300Kg, squeeze in the fermentor tank fully with pump then, subsequently glucose syrup is gone into test tank from the glucose syrup tank pressure, be depressed into 1400Kg always, then with steam flush glucose syrup pipeline, till thoroughly rinsing, then glucose syrup is pumped in the fermentor tank, clean test tank 2-5 time with tap water again, the water of beginning to drink is then squeezed into fermentor tank to accumulative total water consumption 38000L, drop into 49Kg ammonium sulfate from the fermentor tank mouth simultaneously, 4.5Kg bubble enemy (according to the material situation, and during sterilization foam what, can suitably increase or reduce bubble enemy's usage quantity, scope 4.5～14Kg) and 200～300Kg (be designated as W
1) the Wo Shi thing, after all materials all drop in the fermentor tank on request, close the dnockout pump, claim to shut cover behind an amount of sheet alkali accent PH to 4.2～4.4, open to stir and prepare sterilization.
2.2 sterilization:
2.2.1 substratum sterilization: open the coil pipe water discharging valve, again opening quotation pipe steam heating.Control steam total pressure is at 0.1～0.25Mpa, when treating that a jar temperature reaches 100 ℃, stop stirring, close coil pipe steam, using three road steam instead directly sterilizes, open bottom steam earlier, open blast main steam again, open stopple coupon steam at last, slightly open exhaust-valve, it is an amount of to open each drain tap of fermentor tank can, regulates pot liquid and acutely seethes, and makes it even; When tank pressure reaches 0.08Mpa, control three road steam stagnation pressures, when 118 ℃ of jar Nei Wenduda, begin insulation, 118～120 ℃ of controlled temperature, 20～30 minutes time, pressure-controlling is at 0.09～0.10MPa; Pressurize is closed each drain tap after finishing, and closes three road steam (order is followed successively by: stopple coupon, bottom, air distribution pipe) again, and opening quotation pipe recirculated water cools off, and opens the air exhaust-valve, and when tank pressure was reduced to 0.03～0.05Mpa, logical pressurized air carried out pressurize; When temperature is reduced to 35 ℃, get substratum and survey residual sugar content, inoculate when treating a jar temperature drop to 30 ± 1 ℃.
2.2.2 inoculation: check temperature in the jar before the inoculation, whether tank pressure is normal, when temperature is reduced to 26 ± 1 ℃,, close the air inlet valve with inoculation mouth on the 75% ethanol wiping jar, when the fast emptying of fermentor tank gas, rapidly pyrosphere is placed on the inoculation mouth, waits jar interior gas to arrange, light pyrosphere, be with the gloves of preventing fires, to plant liquid and pour in the fermentor tank, after having inoculated, wrap pyrosphere with wet cotton from flame hole, shut the inoculation mouth, open stirring, drive the air inlet valve, regulate vent valve, control tank pressure and flow well, enter the fermentation culture process.
2.3 fermentation culture:
2.3.1 enter the spawn culture phase after the inoculation, the incubation period temperature is controlled at 26 ± 1 ℃, pressure-controlling is at 0.06 ± 0.01MPa.
2.3.2, control the air flow quantity of incubation period by following requirement according to present Wo Shi charging capacity:
| Incubation time (h) | Air flow quantity (m 3/h) |
| ??1～2 | ??500 |
| ??3～14 | ??600～700 |
| 15～finish | ??700～800 |
2.4 feed intake:
2.4.1 supplementary material proportioning and charging capacity
2.4.2 preparation work: add 1000～1500kg tap water in charging tank, be warming up to 100 ℃ of postcooling to 70～80 ℃, the back adds washing powder 10kg, the Wo Shi thing W after will pulverizing then
2=(600～720-W
1) kg drops in the charging tank, open stirring, close cover,, begin sampling and do transformation efficiency after 24 hours at spawn culture, and detect PH with the PH meter, change when rising and to feed intake when PH descends, general transformation efficiency at this moment when detecting data near feeding point, carries out disinfection until feeding intake to the pipeline that feeds intake with high pressure steam about 20～35%.
2.4.3 feed intake: when possessing the condition of feeding intake, begin to feed intake, the pressure of control fermentor tank is at 0.03Mpa when feeding intake, open the refrigerated water cooling,, open charging tank binder pipe valve when charging tank pressure rises to 0.10～0.15Mpa, feed liquid is pressed into fermentor tank, regulate charging tank air door control charging time 10～20 minutes, feed intake finish after, the air flow quantity 750～800m of control fermentor tank
3/ h, temperature is at 26 ± 1 ℃, pressure 0.03 ± 0.01Mpa.
2.5 fermentation oxidising process:
2.5.1 by present charging capacity, oxidation period, temperature, pressure and the flow control of different steps were as follows:
| Time (h) | Pressure (Mpa) | Air flow quantity (M 3/h) | Temperature (℃) |
| ??0-2 | ??0.03 | ??1000-1050 | ??26±1 |
| ??3-14 | ??0.06 | ??1000-1050 | ??26±1 |
| ??11-20 | ??0.06 | ??1100-1200 | ??26±1 |
2.5.2 begin sampling behind the oxidation 30h, sampling is every two hours once done microscopy, and detects transformation efficiency and PH, purpose is that oxidation situation in the jar is done preliminary understanding, mainly surveys its whether microbiological contamination and conversion situation, and unusual circumstance can in time solve; And with the timely record of every data, the bacterium inspection finds that the microbiological contamination situation is arranged, and should in time take measures, and weight according to circumstances determines whether putting jar; If microscopy is only found seldom assorted bacterium, and filtering velocity is good, but proper extension oxidization time; If microscopy finds that situation is more serious, should in time heat and put jar; If microscopy is normal, pH value in allowed band and filtering velocity good, oxidation after 30～40 hours i.e. heating put jar.
2.6 put jar:
Finish oxidation period to take a sample with beaker before the heating, send quality inspection to survey residual sugar content, begin heating after the sampling, close recirculated water or refrigerated water inlet valve, open the coil pipe sewage draining valve, the pipe steam that opens the set heats, and after Heating temperature rises to 75 ℃ feed liquid is pressed into the transfer jar.
2.7 Plate Filtration:
2.7.1 filter: treat that fermented liquid is pressed into blowing behind the transfer jar, open the fluid valve that to ferment of pressure filter during blowing, turn off air door and high-pressure hydraulic pump valve, open return valve, utilize the press filtration of tank pressure elder generation about 20 minutes; When the discharge of finding liquid-outlet valve is too small, turn off reverse flow valve, open high-pressure pump, control pressure≤0.8Mpa, during press filtration, check whether liquid-outlet valve has mycelia to overflow, overflow as finding mycelia, must turn off feed valve at once, and blow for some time with pressurized air, and pressure testing filter again; When liquid outlet also seldom has fermented liquid to come out, illustrate that mycelia press dry, turn off high-pressure hydraulic pump, dry up with pressurized air; The back is delivered between pulverizing unloading down on the mycelia slave plate.
2.7.2 pulverize: filter cake is ground into meal with Roughpulverizer earlier, is ground into fine powder with slimer again, pulverized the back pack, fasten sack, must contain the mold oxide mycelia.
Claims (4)
1. a microorganism fermentation process comprises the steps:
A, cultivation seed, it comprises the steps: again
1. dispose substratum: get millet 0.0001～0.0002%, cleaning the back soaked 25～35 minutes, then millet is cooked, and control pressure is to carry out pressurize sterilization 10～20 minutes under the condition of 100～115 ℃ of 0.04～0.06Mpa, temperature, pulverize in the eggplant bottle of packing into after then the millet that cooks being taken out and carry out disinfection, the sterilization postcooling is during to normal temperature, the millet in the bottle unfolded put tiltedly that to constitute millet seed inclined-plane be substratum (weight percent);
2. carry out sterilisable chamber operation: in sterilisable chamber millet seed inclined-plane is moved to the blank inclined-plane of millet, be placed on after finishing in the permanent room temperature and cultivated 8～12 days, will cultivating bread mould seed after 8～12 days then, to be placed on temperature be to preserve in 0～6 ℃ the refrigerator;
3. scrape kind: the bread mould seed on the seed inclined-plane is scraped in the bottle and break up even one-tenth bacteria suspension with scraper, seal with tampon then, scrape remaining seed inclined-plane with method, above-mentioned bacteria suspension is put into the Erlenmeyer flask of containing sterilized water for per 4～8 bottles synthetic one bottle and is waited for inoculation;
B, fermentation sterilization, it also comprises the steps:
1. batching: by the glucose syrup of total amount 3.5～4%, 3.3～3.6% corn steep liquor, 0.11～0.15% ammonium sulfate, 0.010～0.015% bubble oppose, (Wo Shi thing weight is designated as W for 0.50%～0.85% Wo Shi thing
1), residual content is that the composition of water drops into fermentor tank, then adds to shut cover and open stirring after alkali is transferred PH to 4.2～4.4, then carry out disinfection and inoculate (weight percent);
2. fermentation culture and feeding intake: inoculation earlier, after carry out spawn culture, the temperature of spawn culture is controlled at 26 ± 4 ℃, pressure-controlling is at 0.06 ± 0.10MPa, and is 450～550m by the air flow quantity of incubation time in 1～2h
3/ h, the incubation time air flow quantity in 3～14h is 600～700m
3/ h, incubation time are 700～800m in the air flow quantity of 15h～before feeding intake
3/ h requires the control air flow; Adding weight before beginning to feed intake in charging tank is 3～6W
1Water, add washing powder 0.0024～0.048%, be 0.8～2.0W with weight then
1The Wo Shi thing pulverize the back and drop in the charging tank, open stirring, close cover and the sterilization pipeline that feeds intake, feed liquid can be pressed into fermentor tank (weight percent) when PH descends to changeing when rising;
3. ferment oxidation and put jar: feeding intake enters oxidation period after finishing, behind oxidation 25～35h, begin to do microscopy, seriously should in time put jar if find microbiological contamination, finish sampling oxidation period and detect residual sugar content, begin heating after the sampling, stop after Heating temperature rises to 70～80 ℃ to stir and carry out binder, strengthen tank pressure during binder and guarantee that a feed liquid is seethed in the jar, then feed liquid is pressed into the transfer jar;
4. Plate Filtration: fermented liquid carries out blowing after being pressed into the transfer jar, utilizes tank pressure to carry out press filtration earlier during blowing, when mycelia press dry, dries up with pressurized air, unloading down on the mycelia slave plate, filter cake is ground into fine powder then, has pulverized the back pack.
2. microorganism fermentation process according to claim 1; it is characterized in that described carrying out in the sterilisable chamber operation; the operation steps that millet seed inclined-plane is moved to the blank inclined-plane of millet is: open the seed bottle; insert big bacterial classification of soya bean of seed bottle picking with the culture transferring pin; bacterial classification is put into adds sterilized water in the sterilized water again until watching spore to be as the criterion with naked eyes; make another parallel sample with method; draw the above-mentioned bacterium liquid that makes with suction pipe respectively according to the aseptic technique method then, and move on on the blank millet inclined-plane.
3. microorganism fermentation process according to claim 1 is characterized in that in the described fermentation disinfectant program, and the operation steps of sterilization and inoculation is:
A, pressurize sterilization: open steam heating, control steam total pressure is at 0.1～0.25Mpa, and stirring, when treating that a jar temperature reaches 100 ℃, stops stirring, and directly sterilizes with steam, regulates pot liquid and acutely seethes, and makes it even; When tank pressure reaches 0.08Mpa, control steam stagnation pressure; When 118 ℃ of jar Nei Wenduda, begin insulation, 118～120 ℃ of controlled temperature, 20～30 minutes time, pressure-controlling is at 0.09～0.10MPa;
B, pressurize finish postcooling, and when tank pressure was reduced to 0.03～0.05Mpa, logical pressurized air carried out pressurize, inoculate when treating a jar temperature drop to 30 ± 1 ℃;
Whether a temperature in checking before c, the inoculation jar, tank pressure be normal, 26 ± 1 ℃ of inoculation temps, with inoculation mouth on the 75% ethanol wiping jar, close the air inlet valve, when the fast emptying of fermentor tank gas, rapidly pyrosphere is placed on the inoculation mouth, wait jar interior gas to arrange, light pyrosphere, will plant liquid and pour in the fermentor tank from flame hole.
4. microorganism fermentation process according to claim 1, it is characterized in that described Plate Filtration is in pressure-filtering process, need to check whether liquid-outlet valve has mycelia to overflow, as find the mycelia effusion, must turn off feed valve at once, and blow for some time with pressurized air, and pressure testing filter again, as not finding to run material, continue again to press, otherwise stop pressure filter work.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171974A (en) * | 2016-07-04 | 2016-12-07 | 广西大学 | The sterilization of Pseudobulbus Bletillae (Rhizoma Bletillae) the surface of the seed and the method for inoculation |
| CN112410378A (en) * | 2020-11-26 | 2021-02-26 | 江苏星海生物科技有限公司 | Uniformly mixed biological fermentation process for microbial technology |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706961A (en) * | 2004-06-07 | 2005-12-14 | 西安益尔高科技股份有限公司 | Dexamethasone hormone medicine intermediate and its prepn process |
| CN1876808A (en) * | 2006-05-26 | 2006-12-13 | 华东理工大学 | Streptomyces virginiae and its application in transformation and degradation of steroid |
-
2009
- 2009-10-27 CN CN 200910186403 patent/CN101696423B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171974A (en) * | 2016-07-04 | 2016-12-07 | 广西大学 | The sterilization of Pseudobulbus Bletillae (Rhizoma Bletillae) the surface of the seed and the method for inoculation |
| CN112410378A (en) * | 2020-11-26 | 2021-02-26 | 江苏星海生物科技有限公司 | Uniformly mixed biological fermentation process for microbial technology |
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| CN101696423B (en) | 2013-07-24 |
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