CN112403031B - Extraction device and extraction method of medical theaflavin - Google Patents
Extraction device and extraction method of medical theaflavin Download PDFInfo
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- CN112403031B CN112403031B CN202011518074.6A CN202011518074A CN112403031B CN 112403031 B CN112403031 B CN 112403031B CN 202011518074 A CN202011518074 A CN 202011518074A CN 112403031 B CN112403031 B CN 112403031B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
Abstract
The invention discloses a device and a method for extracting medical theaflavin, wherein the extraction device comprises an extraction box, the lower end of the extraction box is provided with a rotating assembly, a chromatographic column is clamped on the rotating assembly, a stabilizing assembly is arranged inside the upper end of the extraction box, the middle part of the stabilizing assembly is provided with a communicating assembly matched with the chromatographic column, the communicating assembly comprises a communicating pipe passing through the middle part of the stabilizing assembly, the upper end of the communicating pipe is communicated with six liquid inlet pipes, the lower end of the communicating pipe is connected with the chromatographic column through a communicating bearing, and the outer ring of the communicating bearing is connected with a shunting box communicated with the communicating pipe. According to the invention, through the arrangement of the auxiliary mechanism, when the solution needing to be extracted with theaflavin is pumped in, the auxiliary mechanism is driven by pressure to compress the filler on the inner side wall of the chromatographic column, so that the tube wall effect is reduced, and the better extraction effect on theaflavin in the solution is ensured.
Description
Technical Field
The invention relates to the technical field of theaflavin extraction, in particular to a device and a method for extracting medical theaflavin.
Background
Theaflavin is a golden pigment present in black tea, is a product of tea fermentation, and has a compound with a definite pharmacological action, but the proportion of theaflavin in the dry tea is very low, only 0.5% to 2%, and the extraction of theaflavin is very difficult; the existing theaflavin extraction mostly adopts column chromatography and high-speed counter-current chromatography, and under the existing conditions, certain disadvantages exist when the high-speed counter-current chromatography is adopted for extracting theaflavin.
The chromatographic column is used when theaflavin is extracted by high-speed counter-current chromatography, the filling conditions of the fillers of the existing chromatographic column are always different between the central part of the column and the part along the tube wall, the part close to the tube wall is loose, channeling is easy to generate, the flow rate is high, the flow shape of a flushing agent is influenced, the spectral band is widened, namely, the tube wall effect is generated, and the extraction effect of the chromatographic column on theaflavin is seriously influenced by the existence of the tube wall effect.
The invention aims to provide a medical theaflavin extraction device to overcome the defect of poor theaflavin extraction effect caused by the tube wall effect.
Disclosure of Invention
The invention aims to provide a medical theaflavin extraction device to solve the problem of poor theaflavin extraction effect caused by the tube wall effect in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a theaflavin extraction element is used to medicine, is including drawing the case, it is provided with the runner assembly to draw the case lower extreme, the last joint of runner assembly has the chromatographic column, it is equipped with firm subassembly to draw the inside intercommunication subassembly that is equipped with of case upper end, firm subassembly middle part be provided with the chromatographic column suits.
The intercommunication subassembly is including passing the communicating pipe at firm subassembly middle part, communicating pipe upper end intercommunication has six feed liquor pipes, communicating pipe lower extreme through the intercommunication bearing with the chromatographic column is connected, intercommunication bearing inner race be connected with the reposition of redundant personnel box that is linked together communicating pipe, reposition of redundant personnel box lateral wall evenly is equipped with complementary unit along its circumference direction, reposition of redundant personnel box lower extreme intercommunication have with the reposition of redundant personnel mechanism that complementary unit is linked together.
Wherein the auxiliary mechanism comprises an auxiliary hole arranged on the side wall of the shunting box, an L-shaped auxiliary pipe communicated with the shunting box is movably connected in the auxiliary hole, an auxiliary spring is arranged between the L-shaped auxiliary pipe and the inner wall of the auxiliary hole, the lower end of the L-shaped auxiliary pipe is connected with a connecting plate, a circular groove communicated with the L-shaped auxiliary pipe is arranged in the connecting plate, the upper end of the circular groove is in a circular truncated cone shape, a movable rod is movably connected in the circular groove, the lower end of the movable rod passes through the side wall of the lower end of the connecting plate, the upper end of the movable rod is connected with a circular truncated cone-shaped sealing plate which is matched with the inner wall of the upper end of the circular groove, the side wall of the middle part of the movable rod is connected with a sealing piston which is matched with the inside of the circular groove, the lower end of the movable rod is provided with an arc plate, and a reset spring is arranged between the arc plate and the connecting plate.
Preferably, the flow distribution mechanism comprises a cone-shaped sealing plug attached to the lower end of the communicating pipe, the lower end of the cone-shaped sealing plug is connected with a flow distribution plate through a connecting column, water flowing holes are evenly formed in the flow distribution plate, and a flow distribution spring is arranged between the flow distribution plate and the inner wall of the flow distribution box.
Preferably, the rotating assembly comprises a rotating shaft penetrating through the side wall of the lower end of the extraction box, a rotating seat is arranged on the rotating shaft, limit blocks are evenly arranged on the rotating seat, and limit grooves matched with the limit blocks are formed in the lower end of the chromatographic column.
Preferably, the subassembly that stabilizes is including setting up draw the firm board of case upper end inner wall, firm board middle part threaded connection has firm bearing, firm bearing's outer lane lateral wall be equipped with firm board middle part threaded connection's thread tooth, firm bearing's inner circle with communicating pipe lateral wall is connected, firm bearing's outer lane evenly is equipped with the bull stick along its circumference direction.
Preferably, the lower end face of the arc-shaped plate is movably connected with three arc-shaped pressing plates through the protection springs, and the strength of the protection springs of the three arc-shaped pressing plates is gradually enhanced from inside to outside.
Preferably, the side wall of the lower end of the connecting plate is further provided with a water outlet, the water outlet is communicated with the flow distribution box through a hose, and the position where the water outlet is communicated with the flow distribution box is located at the upper end of the flow distribution plate.
Preferably, the elastic strength of the return spring is smaller than that of the shunt spring, and the elastic strength of the shunt spring is smaller than that of the protection spring.
Preferably, the upper end of the flow distribution plate is a plane, and the lower end of the flow distribution plate is of an arc structure.
The invention also provides a theaflavin extraction method adopting the medical theaflavin extraction device, which comprises the following steps:
s1, taking down the communicating component, and placing the auxiliary mechanism and the flow dividing mechanism at the lower end of the communicating component into the chromatographic column;
s2, putting the communicating component and the chromatographic column into an extraction box, limiting the chromatographic column on the rotating component at the moment, and connecting the outer side wall of the stabilizing bearing with the stabilizing plate through threads;
s3, rotating the stabilizing bearing according to the height and the fluffiness of the filler in the chromatographic column to enable the distance between the lower end of the auxiliary mechanism and the filler in the chromatographic column to be 1 cm;
s4, pumping the prepared shunting solution and the solution needing to extract theaflavin into a chromatographic column by using a pump;
s5, collecting the solution passing through the chromatographic column from a liquid outlet at the lower end of the extraction box; measuring the absorbance of the collected solution in each test tube at 380 nm;
s6, respectively collecting different components in the solution, concentrating, freeze drying, and recrystallizing to obtain pure theaflavin extract.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, through the arrangement of the auxiliary mechanism, when the solution needing to be extracted with theaflavin is pumped in, the auxiliary mechanism is driven by pressure to compress the filler on the inner side wall of the chromatographic column, so that the tube wall effect is reduced, and the better extraction effect on theaflavin in the solution is ensured.
2. According to the invention, through the matching of the auxiliary mechanism and the flow dividing mechanism, the solution to be extracted with theaflavin can flow out from the middle part of the chromatographic column, and the tube wall effect is reduced from another angle, so that the better extraction effect of theaflavin in the solution is ensured.
3. The invention also ensures that the chromatographic column keeps stable during high-speed rotation through the arrangement of the rotating assembly and the stabilizing assembly, reduces the shaking of the chromatographic column caused by high-speed rotation, and ensures the stable operation of the whole extraction device and the chromatographic column.
Drawings
FIG. 1 is a first internal structural schematic of the present invention.
Fig. 2 is a second internal structural diagram of the present invention.
FIG. 3 is an enlarged view of a portion of FIG. 2 according to the present invention.
Fig. 4 is a partially enlarged view of the position B in fig. 2 according to the present invention.
In the figure: 1 extraction box, 2 rotating assemblies, 21 rotating shaft, 22 rotating seats, 23 limiting blocks, 24 limiting grooves, 3 chromatographic columns, 4 stabilizing assemblies, 41 stabilizing plates, 42 stabilizing bearings, 43 rotating rods, 5 communicating assemblies, 51 communicating pipes, 52 liquid inlet pipes, 53 communicating bearings, 54 shunting boxes, 6 auxiliary mechanisms, 61 auxiliary holes, 62L-shaped auxiliary pipes, 63 auxiliary springs, 64 connecting plates, 65 circular grooves, 66 movable rods, 67 circular table type sealing plates, 68 sealing pistons, 69 arc-shaped plates, 610 return springs, 611 arc-shaped pressing plates, 612 water outlets, 613 hoses, 7 shunting mechanisms, 71 circular table type sealing plugs, 72 connecting columns, 73 shunting plates and 74 shunting springs.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1 to 4, an embodiment of the present invention includes: the utility model provides a theaflavin extraction element for medicine, is provided with runner assembly 2 including drawing case 1, draws 1 lower extreme of case, and the joint has chromatographic column 3 on runner assembly 2, draws 1 inside firm subassembly 4 that is equipped with in case upper end, and firm subassembly 4 middle part is provided with the intercommunication subassembly 5 that suits with chromatographic column 3.
Specifically, rotating assembly 2 cooperatees with firm subassembly 4 and 3 spacing firm with the chromatographic column, can ensure that chromatographic column 3 operates steadily when rotating at a high speed, guarantees the effect of extracting of chromatographic column 3 to theaflavin in the solution.
Referring to fig. 2, the communicating assembly 5 includes a communicating pipe 51 passing through the middle portion of the stabilizing assembly 4, the upper end of the communicating pipe 51 is communicated with six liquid inlet pipes 52, the lower end of the communicating pipe 51 is connected with the chromatographic column 3 through a communicating bearing 53, the outer ring of the communicating bearing 53 is connected with a flow dividing box 54 communicated with the communicating pipe 51, the side wall of the flow dividing box 54 is uniformly provided with the auxiliary mechanisms 6 along the circumferential direction thereof, and the lower end of the flow dividing box 54 is communicated with a flow dividing mechanism 7 communicated with the auxiliary mechanisms 6.
Specifically, the configured theaflavin extraction solution and the solution to be extracted theaflavin are pumped into the communicating pipe 51 from the liquid inlet pipe 52 through the water pump, so as to enter the chromatographic column 3 for extracting theaflavin, in addition, the side wall of the upper end of the communicating pipe 51 is also communicated with the auxiliary pipe 8 for pumping the configured theaflavin extraction solution, and the six liquid inlet pipes 52 are used for pumping the theaflavin solution to be extracted.
Further, when using, take out firm subassembly 4 and intercommunication subassembly 5 earlier, then compression is accomodate among the intercommunication subassembly 5 complementary unit 6, place into chromatographic column 3 inside complementary unit 6, last with firm subassembly 4, in intercommunication subassembly 5 and the chromatographic column 3 dress back draw box 1, at this moment, with chromatographic column 3 lower extreme spacing to rotating assembly 2 on, with firm subassembly 4 in the firm bearing 42 threaded connection of ground to firm board 41, the realization is to the complementary unit 6 and the reposition of redundant personnel mechanism 7 phase-match among chromatographic column 3 and the intercommunication subassembly 5, and with chromatographic column 3 spacing firm.
Referring to fig. 2 and 3, the auxiliary mechanism 6 includes an auxiliary hole 61 formed in a side wall of the diversion box 54, an L-shaped auxiliary tube 62 connected with the diversion box 54 is movably connected in the auxiliary hole 61, an auxiliary spring 63 is arranged between the L-shaped auxiliary tube 62 and an inner wall of the auxiliary hole 61, a connecting plate 64 is connected to a lower end of the L-shaped auxiliary tube 62, a circular groove 65 communicated with the L-shaped auxiliary tube 62 is formed in the connecting plate 64, an upper end of the circular groove 65 is in a circular table shape, a movable rod 66 is movably connected in the circular groove 65, a lower end of the movable rod 66 penetrates through a lower end side wall of the connecting plate 64, a circular table-shaped sealing plate 67 corresponding to an inner wall of an upper end of the circular groove 65 is connected to an upper end of the movable rod 66, a sealing piston 68 corresponding to an inner portion of the circular groove 65 is connected to a middle side wall of the movable rod 66, an arc-shaped plate 69 is installed at a lower end of the movable rod 66, and a return spring 610 is arranged between the arc-shaped plate 69 and the connecting plate 64.
The outer end of the filler in the chromatographic column 3 can be compacted through the auxiliary mechanism 6, and the generation of a tube wall effect is reduced, so that the extraction effect of the chromatographic column 3 on theaflavin is guaranteed.
Specifically, after the prepared theaflavin extraction solution is pumped into the chromatographic column 3, the rotating assembly 2 operates, and the theaflavin solution to be extracted is pumped from the six liquid inlet pipes 52 at the upper end of the communicating pipe 51 at the same time, at this time, because the water outlet 612 on the connecting plate 64 in the auxiliary mechanism 6 is not opened yet, a large pressure is formed in the L-shaped auxiliary pipe 62 and the connecting plate 64 in the auxiliary mechanism 6, when the pressure reaches a certain value, the truncated cone-shaped sealing plate 67 is pushed open, that is, the return spring 610 is compressed and drives the truncated cone-shaped sealing plate 67 and the movable rod 66 to move downwards, and with the increase of the pressure, the movable rod 66 continues to move downwards until the sealing piston 68 on the side wall in the middle of the movable rod 66 moves downwards below the water outlet 612, and the solution enters the diversion box 54 from the circular groove 65 in the connecting plate 64 through the water outlet 612 and the hose 613.
Arc pressing plate 611 is connected to the lower terminal surface of arc 69 through protection spring swing joint, and arc pressing plate 611 is equipped with three, and the protection spring intensity of three arc pressing plate 611 strengthens from inside to outside gradually.
Furthermore, in the process of continuously moving downwards along with the increase of the pressure of the movable rod 66, the arc-shaped plate 69 is driven to move downwards, and when the arc-shaped plate 69 moves downwards, the arc-shaped pressing plate 611 is driven to press the filler on the side wall of the chromatographic column 3, so that the filler on the side wall of the chromatographic column 3 is pressed tightly, and the tube wall effect is reduced.
It should be added that, because the filler in the chromatographic column 3 is compact in the middle, the closer to the side wall, the more the filler needs to be pressed, that is, the higher the pressure required by the filler, therefore, the strength of the protection springs of the three arc-shaped pressing plates 611 is gradually increased from inside to outside, so as to adapt to the condition of the filler in the chromatographic column 3, that is, the phenomenon that the pressure in the auxiliary mechanism 6 is too large because the movable rod 66 cannot move downward due to the compact filler in the chromatographic column 3 is avoided, and the fact that the filler on the inner side wall of the chromatographic column 3 can be pressed tightly is also ensured.
Referring to fig. 2 and 3, the flow dividing mechanism 7 includes a truncated cone shaped sealing plug 71 attached to the lower port of the communicating tube 51, the lower end of the truncated cone shaped sealing plug 71 is connected to a flow dividing plate 73 through a connecting column 72, water flow holes are uniformly formed in the flow dividing plate 73, a flow dividing spring 74 is arranged between the flow dividing plate 73 and the inner wall of the flow dividing box 54, and the flow dividing plate 73 is attached to the inner wall of the flow dividing box 54.
Specifically, when the pressure in the communicating component 5 reaches a certain value, the water outlet 612 in the auxiliary mechanism 6 is opened, the solution enters the diversion box 54, more specifically, the solution enters the space between the diversion box 54 and the upper end of the diversion plate 73, since the diameter of the water outlet 612 is limited, and the elastic coefficient of the return spring 610 is smaller than that of the shunt spring 74, the shunt spring 74 is stretched as the pressure increases, i.e., the truncated cone shaped sealing plug 71 is separated from the lower port of the communicating pipe 51, the solution will enter the diversion box 54 from the communicating pipe 51, more specifically, the solution enters the space at the upper ends of the diversion box 54 and the diversion plate 73, and finally, the solution in the space above the diverter box 54 and the diverter plate 73 flows out from the water flow holes on the diverter plate 73, and the extraction of the theaflavin in the theaflavin solution to be extracted is realized by filtering the filler in the chromatographic column 3.
In the process, no matter the auxiliary mechanism 6 at the side or the flow dividing mechanism 7 at the middle, the solution conveyed into the chromatographic column 3 is conveyed into the chromatographic column 3 from the middle of the chromatographic column 3, and the tube wall effect is reduced from another angle, so that the extraction effect of the chromatographic column 3 is ensured.
Referring to fig. 2 and 4, the rotating assembly 2 includes a rotating shaft 21 penetrating through the sidewall of the lower end of the extracting box 1, a rotating seat 22 is disposed on the rotating shaft 21, a limiting block 23 is uniformly disposed on the rotating seat 22, and a limiting groove 24 corresponding to the limiting block 23 is disposed at the lower end of the chromatographic column 3.
Specifically, place into chromatographic column 3 with complementary unit 6 and reposition of redundant personnel mechanism 7 and in, install chromatographic column 3 and get into and draw case 1, be about to spacing groove 24 and the stopper 23 joint on the seat 22 of rotating of chromatographic column 3 lower extreme to spacing chromatographic column 3, at the during operation, drive whole seat 22 and the high-speed rotation of chromatographic column 3 of rotating through axis of rotation 21.
Referring to fig. 2 and 4, the stabilizing assembly 4 includes a stabilizing plate 41 disposed on the inner wall of the upper end of the extraction box 1, a stabilizing bearing 42 is screwed to the middle of the stabilizing plate 41, a thread tooth screwed to the middle of the stabilizing plate 41 is disposed on the outer side wall of the stabilizing bearing 42, the inner ring of the stabilizing bearing 42 is connected to the outer side wall of the communicating pipe 51, and a rotating rod 43 is uniformly disposed on the outer ring of the stabilizing bearing 42 along the circumferential direction thereof.
Specifically, when installing chromatographic column 3, with chromatographic column 3 spacing to rotating on the seat 22 back, rotate the firm bearing 42 of upside to firm board 41 on, and adjust the arc of auxiliary mechanism 6 lower extreme and press the packing in board 611 and the chromatographic column 3 to suitable distance, receive spacingly when chromatographic column 3 lower extreme, the firm bearing 42 of rethread upper end is spacing again to chromatographic column 3, guaranteed further that chromatographic column 3 is stable when high-speed rotation, stability when improving chromatographic column 3 during high-speed rotation of during operation.
The side wall of the lower end of the connecting plate 64 is further provided with a water outlet 612, the water outlet 612 is communicated with the diversion box 54 through a hose 613, the position where the water outlet 612 is communicated with the diversion box 54 is located at the upper end of the diversion plate 73, the upper end of the diversion plate 73 is of a plane structure, and the lower end of the diversion plate 73 is of an arc structure.
Specifically, the water outlet 612 is arranged at the upper end of the splitter plate 73, and the upper end of the splitter plate 73 is of a planar structure, so that when the solution is gathered between the splitter boxes 54 and the splitter plates 73, the pressure stably enters the ground fillers in the chromatographic column 3, and the lower end of the splitter plate 73 is arranged in an arc-shaped structure, so as to adapt to the tight and tight situation of the fillers in the chromatographic column 3, because the fillers in the inner side wall of the chromatographic column 3 are easily compressed and the middle part is tight and not easily compressed, when the fillers in the inner side wall of the chromatographic column 3 are compressed by the auxiliary mechanism 6, the splitter plate 73 of the arc-shaped structure can adapt to the tight and tight situation of the fillers in the chromatographic column 3, thereby not only ensuring that the splitter mechanism 7 and the auxiliary mechanism 6 can be matched with each other to compact the fillers in the chromatographic column 3, but also ensuring that the solution enters the fillers from the middle part of the chromatographic column 3 under the same pressure, and ensuring the extraction effect of the chromatographic column 3, reducing the generation of the tube wall effect.
The return spring 610 has an elastic strength smaller than that of the shunt spring 74, and the shunt spring 74 has an elastic strength smaller than that of the shield spring.
Specifically, through the setting to reset spring 610, reposition of redundant personnel spring 74 and protection spring elastic strength, can ensure when intercommunication subassembly 5 internal pressure increases gradually, complementary unit 6, reposition of redundant personnel mechanism 7 and arc press plate 611 mutually support in proper order and carry out work, ensure the smooth operation of whole device.
The embodiment also provides a theaflavin extraction method using the above medical theaflavin extraction device, and the extraction method includes the following steps:
s1, taking down the communication component 5, and placing the auxiliary mechanism 6 and the flow dividing mechanism 7 at the lower end of the communication component 5 into the chromatographic column 3;
s2, the communicating component 5 and the chromatographic column 3 are arranged in the extraction box 1 together, at the moment, the chromatographic column 3 is limited on the rotating component 2, and the outer side wall of the stabilizing bearing 42 is in threaded connection with the stabilizing plate 41;
s3, rotating the stabilizing bearing 42 according to the height and fluffiness of the filler in the chromatographic column 3, so that the distance between the lower end of the auxiliary mechanism 6 and the filler in the chromatographic column 3 is 1 cm;
s4, pumping the prepared shunting solution and the solution needing to extract theaflavin into the chromatographic column 3 by using a pump;
s5, collecting the solution passing through the chromatographic column 3 from a liquid outlet at the lower end of the extraction box 1; measuring the absorbance of the collected solution in each test tube at 380 nm;
s6, respectively collecting different components in the solution, concentrating, freeze drying, and recrystallizing to obtain pure theaflavin extract.
Specifically, the extraction method for extracting theaflavin can greatly reduce the tube wall effect of the existing chromatographic column 3, ensure the use effect of the chromatographic column 3 and ensure the extraction effect of the chromatographic column 3 on theaflavin from different aspects and different angles.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. A theaflavin extraction element is used in medicine, includes and draws case (1), its characterized in that: a rotating assembly (2) is arranged at the lower end of the extraction box (1), a chromatographic column (3) is clamped on the rotating assembly (2), a stabilizing assembly (4) is arranged inside the upper end of the extraction box (1), and a communicating assembly (5) matched with the chromatographic column (3) is arranged in the middle of the stabilizing assembly (4);
the communication assembly (5) comprises a communication pipe (51) penetrating through the middle part of the stabilizing assembly (4), the upper end of the communication pipe (51) is communicated with six liquid inlet pipes (52), the lower end of the communication pipe (51) is connected with the chromatographic column (3) through a communication bearing (53), the outer ring of the communication bearing (53) is connected with a distribution box (54) communicated with the communication pipe (51), the side wall of the distribution box (54) is uniformly provided with auxiliary mechanisms (6) along the circumferential direction of the side wall, and the lower end of the distribution box (54) is communicated with a distribution mechanism (7) communicated with the auxiliary mechanisms (6);
wherein the auxiliary mechanism (6) comprises an auxiliary hole (61) arranged on the side wall of the shunting box (54), an L-shaped auxiliary pipe (62) communicated with the shunting box (54) is movably connected in the auxiliary hole (61), an auxiliary spring (63) is arranged between the L-shaped auxiliary pipe (62) and the inner wall of the auxiliary hole (61), the lower end of the L-shaped auxiliary pipe (62) is connected with a connecting plate (64), a circular groove (65) communicated with the L-shaped auxiliary pipe (62) is arranged in the connecting plate (64), the upper end of the circular groove (65) is in a circular table shape, a movable rod (66) is movably connected in the circular groove (65), the lower end of the movable rod (66) penetrates through the side wall of the lower end of the connecting plate (64), the upper end of the movable rod (66) is connected with a circular table-shaped sealing plate (67) matched with the inner wall of the upper end of the circular groove (65), the side wall of the middle part of the movable rod (66) is connected with a sealing piston (68) which is adaptive to the inside of the circular groove (65), the lower end of the movable rod (66) is provided with an arc-shaped plate (69), and a return spring (610) is arranged between the arc-shaped plate (69) and the connecting plate (64);
the flow distribution mechanism (7) comprises a cone-shaped sealing plug (71) attached to a lower port of the communication pipe (51), the lower end of the cone-shaped sealing plug (71) is connected with a flow distribution plate (73) through a connecting column (72), water flowing holes are evenly formed in the flow distribution plate (73), and a flow distribution spring (74) is arranged between the flow distribution plate (73) and the inner wall of the flow distribution box (54).
2. The device for extracting theaflavins for medicine according to claim 1, wherein: rotating assembly (2) is including passing axis of rotation (21) of drawing case (1) lower extreme lateral wall, be provided with on axis of rotation (21) and rotate seat (22), evenly be equipped with stopper (23) on rotating seat (22), chromatographic column (3) lower extreme be equipped with stopper (23) adapted spacing groove (24).
3. The device for extracting theaflavins for medicine according to claim 1, wherein: firm subassembly (4) is including setting up draw firm board (41) of case (1) upper end inner wall, firm board (41) middle part threaded connection has firm bearing (42), the outer lane lateral wall of firm bearing (42) be equipped with firm board (41) middle part threaded connection's thread tooth, the inner circle of firm bearing (42) with communicating pipe (51) lateral wall is connected, the outer lane of firm bearing (42) evenly is equipped with bull stick (43) along its circumference direction.
4. The device for extracting theaflavins for medicine according to claim 1, wherein: arc pressing plates (611) are movably connected to the lower end face of the arc plate (69) through protection springs, three arc pressing plates (611) are arranged, and the strength of the protection springs of the three arc pressing plates (611) is gradually enhanced from inside to outside.
5. The device for extracting theaflavins for medicine according to claim 1, wherein: the side wall of the lower end of the connecting plate (64) is also provided with a water outlet (612), the water outlet (612) is communicated with the diversion box (54) through a hose (613), and the position where the water outlet (612) is communicated with the diversion box (54) is positioned at the upper end of the diversion plate (73).
6. The device for extracting theaflavins for medicine according to claim 1, wherein: the elastic strength of the return spring (610) is smaller than that of the shunt spring (74), and the elastic strength of the shunt spring (74) is smaller than that of the protection spring.
7. The device for extracting theaflavins for medicine according to claim 1, wherein: the upper end of the flow distribution plate (73) is of a plane structure, and the lower end of the flow distribution plate is of an arc structure.
8. A method for extracting theaflavin by using the device for extracting theaflavins for pharmaceutical use according to any one of claims 1 to 7, characterized by comprising: the extraction method comprises the following steps:
s1, taking down the communication component (5), and placing the auxiliary mechanism (6) and the diversion mechanism (7) at the lower end of the communication component (5) into the chromatographic column (3);
s2, the communication component (5) and the chromatographic column (3) are arranged in the extraction box (1) together, at the moment, the chromatographic column (3) is limited on the rotating component (2), and the outer side wall of the stabilizing bearing (42) is in threaded connection with the stabilizing plate (41);
s3, rotating the stabilizing bearing (42) according to the height and the fluffiness of the filler in the chromatographic column (3) to enable the lower end of the auxiliary mechanism (6) to be 1 cm away from the filler in the chromatographic column (3);
s4, pumping the prepared shunting solution and the solution needing to extract theaflavin into a chromatographic column (3) by using a pump;
s5, collecting the solution passing through the chromatographic column (3) from a liquid outlet at the lower end of the extraction box (1); measuring the absorbance of the collected solution in each test tube at 380 nm;
s6, respectively collecting different components in the solution, concentrating, freeze drying, and recrystallizing to obtain pure theaflavin extract.
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| CN202011518074.6A CN112403031B (en) | 2020-12-21 | 2020-12-21 | Extraction device and extraction method of medical theaflavin |
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| CN202011518074.6A CN112403031B (en) | 2020-12-21 | 2020-12-21 | Extraction device and extraction method of medical theaflavin |
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| CN112403031B true CN112403031B (en) | 2022-02-22 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205668931U (en) * | 2016-06-14 | 2016-11-02 | 回音必集团(江西)东亚制药有限公司 | A kind of constrictor buffering anti-collision device of filling machine |
| CN108577160A (en) * | 2018-07-08 | 2018-09-28 | 安庆市金丝制刷有限公司 | It is a kind of can liquid storage paintbrush |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB646825A (en) * | 1943-10-21 | 1950-11-29 | Fiat Spa | Improvements relating to fluid pressure transmission systems |
| TW200734026A (en) * | 2006-01-31 | 2007-09-16 | Mallinckrodt Baker Inc | End-cap device for high efficiency disposable chromotography column |
| CN208320050U (en) * | 2018-04-08 | 2019-01-04 | 柳挺 | A kind of traditional Chinese medicine extraction blending tank |
| CN209264661U (en) * | 2018-12-27 | 2019-08-16 | 成都普睿法药物研发有限公司 | It is a kind of to prepare chromatographic column filling device |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205668931U (en) * | 2016-06-14 | 2016-11-02 | 回音必集团(江西)东亚制药有限公司 | A kind of constrictor buffering anti-collision device of filling machine |
| CN108577160A (en) * | 2018-07-08 | 2018-09-28 | 安庆市金丝制刷有限公司 | It is a kind of can liquid storage paintbrush |
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