CN112385656A - Efficient attractant for athetis lepigone and application of efficient attractant - Google Patents
Efficient attractant for athetis lepigone and application of efficient attractant Download PDFInfo
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- CN112385656A CN112385656A CN201910765252.6A CN201910765252A CN112385656A CN 112385656 A CN112385656 A CN 112385656A CN 201910765252 A CN201910765252 A CN 201910765252A CN 112385656 A CN112385656 A CN 112385656A
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- attractant
- athetis lepigone
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/10—Catching insects by using Traps
- A01M1/106—Catching insects by using Traps for flying insects
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- Insects & Arthropods (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses a athetis lepigone efficient attractant and application thereof, and belongs to the technical field of biological control. Wherein the attractant has a general formula of ROCOCnHaXbIn the general formula of the attractant, n =2, 3, 4, X = F, Cl, Br, a = 1-7, and b = (2n-1) -a. The athetis lepigone attractant can effectively trap and kill male insects of the athetis lepigone, reduce mating frequency and generation alternation times, and control the generation amount of the athetis lepigone to be below an economic threshold value, thereby achieving the purpose of preventing and treating the athetis lepigone. The invention has the advantages of small dosage, stable chemical property and environmental protection, and can be accurately applied to insect situation prediction, mass trapping and mating interference of athetis lepigone, and the application of the invention to the insect situation prediction, mass trapping and mating interference of athetis lepigoneThe comprehensive treatment of athetis lepigone is of great significance.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a high-efficiency attractant for athetis lepigone and application thereof.
Background
Athetis lepigone (Moschler) belongs to Lepidoptera, the family of noctuidae, and is mainly distributed in Japan, Korea, Russia, Europe, etc. In 2011, athetis lepigone invades China and becomes one of the important pests in summer corn areas in China. The Athetis lepigone mainly damages the roots and stems of the surface layer of corn soil, so that corn plants cannot grow normally, heavy people cause seedling shortage and ridge breaking, and field re-cultivation needs to be damaged when the harm is serious. Traditionally, chemical prevention and control such as spraying, poison baits, poison soil and pesticide filling are mostly adopted, but a great amount of chemical pesticides are used, so that the athetis lepigone can generate drug resistance, and the threat to human health and environmental safety can be caused.
The regulation and control of insect behaviors by chemical substances for pest monitoring and biological control are increasingly paid more attention by agricultural workers. The insect attractant is a form of insect sex pheromone analogues, and can attract adult target insects to reach a preset attracting place for trapping and killing, so that the meeting probability of male and female insects is reduced, mating is disturbed, the reproduction rate is reduced, and pests are controlled below an economic threshold value, so that the purpose of preventing and controlling the pests is achieved.
Disclosure of Invention
The invention aims to provide a high-efficiency attractant for athetis lepigone and application thereof, which are used for insect situation prediction, pest quarantine, mating interference and mass trapping of the athetis lepigone and achieve the ideal effect of comprehensively treating the athetis lepigone.
The invention provides a high-efficiency attractant for athetis lepigone, and the general formula of the attractant is ROOCnHaXbIn the general formula, R is cis-9-tetradecenyl, X is halogen atom, N, a and b are formed by N, wherein N is more than or equal to 2 and less than or equal to 4, a + b is 2N-1, a is more than or equal to 0 and less than 2N-1, and b is more than 0 and less than or equal to 2N-1.
Preferably, in the general formula of the attractant, n is 2, 3 and 4, X is F, Cl and Br, a is 1-7, and b is (2n-1) -a; the efficient attractant for the athetis lepigone is one or more of the above.
More preferably, the high-potency attractant comprises cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate.
The invention also discloses a preparation method of the attractant core of the efficient attractant for the athetis lepigone, which comprises the steps of dripping n-hexane solution containing the athetis lepigone attractant onto the attractant core, wherein the attractant core is a cylindrical concave rubber plug, is 0.5-2 cm high and 0.5-2 cm in bottom diameter, namely a trans-rubber head, dripping a certain amount of n-hexane onto the attractant core after the n-hexane is volatilized, preparing the attractant core containing the athetis lepigone attractant after the n-hexane is completely volatilized, and storing the attractant core in a refrigerator at the temperature of-20 ℃ for later use.
The application of the efficient athetis lepigone attractant in biological control of athetis lepigone is used for pest quarantine, pest situation monitoring, mass trapping and mating interference of athetis lepigone.
Compared with the prior art, the invention has the following beneficial effects: 1) the athetis lepigone attractant can trap a large amount of male athetis lepigone so as to kill physically, can reduce the generation amount of offspring greatly, and control the quantity of the athetis lepigone below an economic threshold; 2) the athetis lepigone attractant disclosed by the invention is stable in chemical property, long in field persistent period, cheap and easily available in raw materials, small in dosage and environment-friendly. 3) The athetis lepigone attractant can be accurately applied to insect pest forecast and pest quarantine, and achieves the ideal effect of comprehensively treating athetis lepigone.
Drawings
FIG. 1 is a chemical structure diagram of a general formula of the attractant component of Athetis lepigone.
FIG. 2 is a schematic diagram of a synthetic route of the attractant cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate.
Detailed Description
The present invention is described in detail below with reference to the attached drawings and the specific embodiments, but it should be understood that the scope of the present invention is not limited by the specific embodiments.
Example 1 preparation of cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate
The compounds of the invention can be synthesized by the same route, and fig. 2 shows a synthetic method taking cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate as an example.
1.00g (6.49mmol) cis-9-tetradecene-1-ol is dissolved in 27mL anhydrous dichloromethane, 0.77g (7.065mmol) Triethylamine (TEA) is added, the mixture is uniformly mixed, ice bath is carried out to keep the temperature at about 0 ℃, 1.09g (7.065mmol) of 3-methyl-4, 4-difluoro-2-butenoyl chloride is dissolved in 3mL dichloromethane solution, dichloromethane solution containing 3-methyl-4, 4-difluoro-2-butenoyl chloride is dropwise added into the reaction solution, after the dropwise addition, ice bath is carried out for 30min, the reaction is carried out at room temperature overnight, the solvent is removed by reduced pressure rotary evaporation, and the chromatographic column is separated to obtain 1.38g (4.182mmol) of yellow oily matter with the yield of 89.0%.
Cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate:1H-NMR(300MHz,CDCl3)δ:0.88(t,J＝9.00Hz,3H),1.29-1.43(m,14H),2.09(m,3H),2.16(d,J＝12.00Hz,4H),3.97(m,2H),5.34(m,2H)，5.84(m,1H)，6.09(m,1H).13C-NMR(75MHz,CDCl3)δ:5.4,11.4,22.7,25.9,27.7(2C),28.6,29.1(2C),31.9,65.3,116.5,128.6，130.6(2C),149.1,166.0.HRMS(ESI):m/z calcd for[M+Na]+C19H32F2NaO2,353.2370；found,353.2316。
example 2 field bioactivity assay
The lure core of the prepared athetis lepigone attractant is placed in a box-shaped trap, and then the trap is hung on a land where athetis lepigone is more serious. In order to ensure the release effect, 500-1500 mu g of athetis lepigone attractant is placed in each 1mL of rubber lure core. To further illustrate the feasibility of this example, 19 specific formulations are given in table 1:
TABLE 1
Selecting a land plot with more serious Athetis lepigone occurrence, wherein the area is more than 20 mu, arranging traps with the height of about 150cm from the ground and the distance between the traps of about 15m for each formula, randomly placing the traps containing the Athetis lepigone attractant, and setting 3 times for each treatment. After the placement, the number of males of athetis lepigone trapped by one time was investigated every two days, and trapping sites were randomly changed for continuous investigation for 15 days. The results of the investigation are shown in Table 2:
TABLE 2
| Formulation of | Total trapping volume (head) | Average daily trapping amount (first/day) |
| 1 | 0 | 0.0±0.0 |
| 2 | 32 | 2.1±0.89c |
| 3 | 43 | 2.9±3.02 |
| 4 | 98 | 13.2±1.23b |
| 5 | 38 | 2.5±2.21c |
| 6 | 15 | 1.0±0.38c |
| 7 | 10 | 0.7±2.16c |
| 8 | 56 | 3.7±1.73c |
| 9 | 73 | 4.9±2.91bc |
| 10 | 298 | 26.5±3.41a |
| 11 | 48 | 3.2±1.43c |
| 12 | 36 | 2.4±2.56c |
| 13 | 43 | 2.9±0.79c |
| 14 | 72 | 4.8±3.24bc |
| 15 | 73 | 4.9±5.03c |
| 16 | 345 | 28.3±4.63a |
| 17 | 37 | 2.5±2.47c |
| 18 | 49 | 3.3±6.14c |
| 19 | 60 | 4.0±0.79c |
The results in table 2 show that the athetis lepigone attractant has a good trapping effect, wherein cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate has a great difference compared with other attractants, and the trapping effect of the athetis lepigone attractant is remarkably different from that of other attractant components when P is less than 0.05. According to the formula 4, the formula 10 and the formula 16, the amount of the attractant is increased, the number of the trapped male athetis lepigone is gradually increased, and the formula 10 and the formula 16 have obvious difference with the formula 4, but the formula 10 and the formula 16 are compared, so that the attractant dosage is increased, the number of trapped heads can be increased, but the difference does not exist, and the formula 10 has the best attraction effect and the highest prevention effect from the aspect of economy and environmental protection.
The cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate provided by the invention is simple to prepare, and raw materials are easy to obtain; the stability is good, the lasting period is long, and the control effect can be maintained for more than 30 days; the specificity is strong, and resistance of athetis lepigone can not be generated; is harmless to human and livestock and environment-friendly; strong stress resistance and convenient use. The method can be accurately applied to the insect situation prediction, the interference mating and the mass trapping of the athetis lepigone, and has important significance for comprehensively treating the athetis lepigone.
The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and other embodiments of the present invention, and any variations that can be made by those skilled in the art are intended to fall within the scope of the present invention.
Claims (5)
1. An efficient attractant for athetis lepigone, which is characterized in that the general formula of the attractant is ROOCnHaXbIn the general formula, R is cis-9-tetradecenyl, X is halogen atom, N, a and b are epsilon to N, wherein N is more than or equal to 2 and less than or equal to 4, a + b =2N-1, a is more than or equal to 0 and less than 2N-1, and b is more than 0 and less than or equal to 2N-1.
2. A potent attractant for athetis lepigone according to claim 1, wherein n =2, 3, 4, X = F, Cl, Br, a = 1-7, b = (2n-1) -a; the efficient attractant for the athetis lepigone is one or more of the above.
3. A potent attractant for athetis lepigone according to claim 1, wherein the potent attractant comprises cis-9-tetradecene-3-methyl-4, 4-difluoro-2-butenoate.
4. A preparation method of a high-efficiency attractant core for athetis lepigone as claimed in claims 1-3, wherein n-hexane solution containing the athetis lepigone attractant is dripped onto the core, a certain amount of n-hexane is dripped onto the core after the n-hexane is volatilized, the core containing the athetis lepigone attractant is prepared after the n-hexane is completely volatilized, and the core is stored in a refrigerator at-20 ℃ for later use.
5. The use of the athetis lepigone efficient attractant disclosed in claims 1-3 for biological control of athetis lepigone.
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Citations (5)
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|---|---|---|---|---|
| JP2001302507A (en) * | 2000-04-20 | 2001-10-31 | Nof Corp | Perfluorocarbon emulsion |
| JP3289138B2 (en) * | 1998-09-17 | 2002-06-04 | 独立行政法人国際農林水産業研究センター | Sexual lure of Himechimojimadameiga |
| CN102422831A (en) * | 2011-09-30 | 2012-04-25 | 北京中捷四方生物科技有限公司 | Lure core for controlling proxenus lepigone |
| CN107279141A (en) * | 2017-07-21 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of halo parapheromone antagonist for being used to prevent and treat prodenia litura |
| CN107286021A (en) * | 2017-05-29 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of halo parapheromone analog for being used to prevent and treat Xestia c nigrum |
-
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- 2019-08-19 CN CN201910765252.6A patent/CN112385656A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3289138B2 (en) * | 1998-09-17 | 2002-06-04 | 独立行政法人国際農林水産業研究センター | Sexual lure of Himechimojimadameiga |
| JP2001302507A (en) * | 2000-04-20 | 2001-10-31 | Nof Corp | Perfluorocarbon emulsion |
| CN102422831A (en) * | 2011-09-30 | 2012-04-25 | 北京中捷四方生物科技有限公司 | Lure core for controlling proxenus lepigone |
| CN107286021A (en) * | 2017-05-29 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of halo parapheromone analog for being used to prevent and treat Xestia c nigrum |
| CN107279141A (en) * | 2017-07-21 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of halo parapheromone antagonist for being used to prevent and treat prodenia litura |
Non-Patent Citations (1)
| Title |
|---|
| TETSU ANDO等: "Sex Attractants for Male Lepidoptera", 《AGRIC.BIOL.CHEM》 * |
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