CN112370528B

CN112370528B - Pheromone composition with marking function and preparation thereof

Info

Publication number: CN112370528B
Application number: CN202011145704.XA
Authority: CN
Inventors: 包汝泼; 李开刚; 尹子煜; 邵梦瑜; 李晓虹; 舒佳妮; 戚岑聪
Original assignee: Ningbo Sansheng Biotechnology Co ltd
Current assignee: Ningbo Sansheng Biotechnology Co ltd
Priority date: 2020-10-23
Filing date: 2020-10-23
Publication date: 2022-04-08
Anticipated expiration: 2040-10-23
Also published as: CN112370528A

Abstract

The invention relates to a pheromone composition, in particular to a pheromone composition with a marking function and a preparation thereof. The pheromone composition of the present invention comprises: the component A comprises: androstenone and/or androstenol; and B component: one or more of geraniol, tiglic aldehyde, palmitic acid, oleic acid, linoleic acid, capric acid, myristic acid, lauric acid, and 3-methylindole; and C, component C: a colorant. According to the invention, through the combined use of various pheromones and the addition of synergistic components derived from plants, the oestrus rate and the reproductive success rate of sows are improved, and meanwhile, a coloring agent is added for identification so as to improve the oestrus identification efficiency. In addition, in the preparation process of the pheromone composition preparation, the nitrogen is filled to protect the androstenol from being oxidized, so that the stability of the preparation is ensured, the components such as an antioxidant, a preservative and the like are avoided, the product is safer, and the requirements of green production are met.

Description

Pheromone composition with marking function and preparation thereof

Technical Field

The invention relates to a pheromone composition, in particular to a pheromone composition with a marking function and a preparation thereof.

Background

In the field of live pig breeding, estrus identification is one of key links, and accurate estrus identification can determine mating time and improve conception success rate. At present, the oestrus identification of sows mainly adopts methods such as external observation, boar oestrus test, back pressure test and the like. The conventional pig farm checks whether a replacement sow or an adult sow is in the oestrus by using a boar test, but the boar test method is high in cost, the boar needs to be fed and taught independently, the action is single, time and labor are wasted, the boar test efficiency is low, the oestrus sow is difficult to find sometimes, the success rate is difficult to guarantee, a small part of sows still have no response reaction in the boar test, and the missed breeding is easy to cause mismatching and missed breeding for recessive oestrus or insensible sows, and the optimal breeding opportunity can be missed, so that the conception rate is reduced, and great loss is caused.

The existing research shows that the method for testing whether the sows oestrus by simulating the sound and the smell of the boars is an effective identification method and has high conception rate. Pheromones which appear in the beginning of the fifties of the last century are chemical pheromones which can stimulate the sexual reaction of animals with the same sex. Androstenone and androstenol secreted by the salivary glands of the boars are the main odor sources of the pigs, and can stimulate oestrus behaviors of the sows such as dorsal arching or upright reflex. Currently, efforts are being made to detect changes in oestrus behavior in sows by using boar pheromones and/or steroid hormones to enhance oestrus performance in sows. But the effect in practical application is still not ideal, products on the market are few, and the problem of how to improve the oestrus rate and the breeding success rate of sows still needs to be solved.

Chinese patent CN108289898B uses aromatic heterocyclic compound quinoline, androstenone and androstenol together, and adds aromatic, antiseptic and antioxidant to make into pheromone composition for improving reproductive success rate and productivity, but quinoline compounds have certain inhalation toxicity and ecological toxicity, and improper use not only affects health of animals and technicians, but also affects ecological environment. The aromatic heterocyclic compound may also interfere with the growth and development of female animals, brings about food safety problems, and is not suitable for the breeding and animal husbandry.

In addition, in order to improve the stability of the product and prolong the storage time, other additives such as preservatives are often added into the preparation of the existing pheromone composition, and the development and growth of sows and fetuses thereof can be influenced by taking the preservatives for a long time, so that the health burden of a human body can be increased along with the accumulation of a food chain in the past, and the existing pheromone composition does not meet the requirement of green cultivation.

Disclosure of Invention

The invention aims to solve the technical problems and provides a green pheromone composition which can improve the oestrus rate and the reproductive success rate of sows and has a recognition function and a preparation thereof.

The above object of the present invention is achieved by the following technical solutions:

a pheromone composition with a labeling function, comprising:

the component A comprises: one or more of androstenone, androstenol, androsterone, androstatetraene, 3 beta-androstenol, pheromone, 4, 16-androstadiene-3 beta-ol, 5, 16-androstadiene-3 beta-ol, estratetraene, and androstan-3-one;

and B component: one or more of geraniol, tiglic aldehyde, palmitic acid, oleic acid, linoleic acid, capric acid, myristic acid, lauric acid, and 3-methylindole;

and C, component C: a colorant.

Further, the component A of the invention is androstenone and/or androstenol.

Further, the component A is a mixture of androstenone and androstenol, wherein the mass ratio of androstenone to androstenol is (8-15): 1.

Furthermore, the mass ratio of the component A to the component B is 1 (1-30).

Further preferably, the mass ratio of the component A to the component B is 1 (15-25).

The component A of the invention is pig pheromone, and has certain promotion effect on stimulating oestrus of sows. The combination of the androstenone and the androstenol can make the smell of the composition closer to the taste of a boar, can better stimulate the sow and improve the mating estrus rate. It was also found in earlier trials that the mixture of androstenone and androstenol works better and the average excitation time is shorter than the use of androstenone or androstenol alone.

In the invention, aromatic heterocyclic compounds such as quinoline are not used, but synergistic components from natural plant extracts are added on the A component. In actual breeding production, factors causing the sow not to oestrus are complex, and besides internal factors such as variety and congenital defects, a large part of the factors are also influenced by external factors such as malnutrition, environmental factors or diseases. Inadequate feeding or long-term exposure to unpleasant conditions can lead to sow over-lean or inadequate nutrient intake, thereby inhibiting the normal physiological function and estrous activity of the ovary, resulting in low estrous rate and difficult reproduction.

The geraniol can be mainly separated from the volatile oil of geranium, lemongrass, roses and other plants, and has mild and sweet rose smell, so that the sow appetite and happiness can be stimulated, the pleasant mental state of the sow can be kept, the food intake is increased, the nutritional status is improved, the immunity is improved, the problem of oestrus difficulty caused by external factors is solved, and the oestrus rate of the sow is improved. The 3-methylindole (skatole) is mainly derived from civet, cheese, milk and excrement, has jasmine fragrance after being diluted, and can play an exciting role after being added in a small amount. The angelica aldehyde can be obtained from natural products such as onion, mint, coffee, fried hazelnut and the like, has special faint scent and is slightly soluble in water, so that the pigs can feel pleased by adding a small amount of the angelica aldehyde and can enter the oestrus state more easily. Capric acid, myristic acid, and lauric acid have fragrance under low concentration condition, and can also play similar role. Palmitic acid, oleic acid and linoleic acid in the component B are ubiquitous in animal and vegetable oil and fat, are important fatty acid components, can play a role in relieving emotion, and can also be used as an emulsifier to improve the dispersibility of the components and improve the stability. The component B is from oil or volatile oil components of plants, is matched with the component A, and can effectively reduce the stress response of sows and improve the physical and mental states of the sows through the combination of different plant and animal information, thereby promoting the oestrus.

Further, the component C is a natural colorant, and the natural colorant is at least one of monascus red, paprika red, caramel pigment, alkannin, beet red, gardenia blue, gardenia yellow, curcumin, zeaxanthin, tea green pigment, astaxanthin and algae blue.

Further, the component C is a synthetic colorant, and the synthetic colorant is at least one of brilliant blue, indigo, amaranth, carmine, new red, erythrosine, allura red, lemon yellow, sunset yellow and grass green.

The colorant of the present invention can rapidly bind to proteins in the skin and act as a label for a certain period of time. The coloring agents with different colors can generate the same effect after contacting the skin of the animal, and the reaction is fast and stable, so that the animal presents stable color on the face, particularly the nose, but the attachment time is different. Due to the marking function of the colorant, technicians do not need to check the ear number repeatedly during emotion check, the emotion check operation process is simplified, and great convenience is brought to the expanded production of live pig breeding.

In the preferred embodiment of the invention, coloring agents with different colors can be added according to pheromones with different concentrations, so that the coloring agents are convenient for technicians to identify. In practical practice, technicians can also give different concentrations of pheromone compositions according to the difference between different individuals, such as different ages, physical conditions, weights, behaviors and performances, and distinguish the compositions by different colors, so that the utilization rate of pheromones and the working efficiency of estrus identification are improved.

The invention also provides a pheromone composition preparation which is a liquid preparation comprising any one of the pheromone compositions and a solvent.

Further, the concentration of the component A in the pheromone composition preparation is 0.05-0.2 mg/ml.

Further, the solvent in the pheromone composition preparation is a mixed solvent at least comprising water and an alcohol solvent.

Further preferably, the alcoholic solvent in the present invention is selected from one or more of ethanol, ethylene glycol, propylene glycol, isopropanol, butanol, pentanol, hexanol, heptanol, octanol, dipropylene glycol, and phenethyl alcohol.

Still more preferably, the alcohol solvent in the present invention is a mixture of ethanol and isopropanol, and the ratio of ethanol to isopropanol is (1-3): 1 (g/g).

Further, the pheromone composition preparation is a spray.

Another object of the present invention is to provide a method for preparing a pheromone composition preparation, comprising: sequentially adding the component A, the component B and the component C into an alcohol solvent, and stirring until the components are completely dissolved; adding water, and stirring uniformly to obtain a semi-finished product solution; filling nitrogen into the filling bottle, filling the semi-finished product solution into the bottle, and sealing to obtain the finished product.

The pheromone composition is not added with any preservative or antioxidant, but is filled with nitrogen in a filling bottle before filling, oxygen in the bottle is expelled, and the effect of protecting androstenol from being oxidized is achieved, so that the stability of the preparation is effectively improved. Because the molecular structures of androstenol and androstenone are only different by two hydrogen atoms, the androstenone is easily oxidized to form androstenone after contacting air, although androstenone is also a sex hormone of a boar, the androstenone and the sex hormone of the boar are different in odor, the stimulation effect of a single component on a sow can be obviously reduced, and the simulation effect of the double components is not as good. Therefore, to control the ratio of androstenone to androstenol in the a-component, it is only under the combined effect of the two that the sow receives a signal more effectively resembling the taste of a boar, thereby showing oestrus. The invention avoids the oxidation reaction of androstenol by improving the process, not only ensures the stability of the preparation, but also reduces the use of additives such as preservative, antioxidant and the like, avoids the influence on the health of animals and people, and better meets the requirement of green cultivation.

Further, in the preparation process of the pheromone composition preparation, the stirring speed is 150-200 rpm, and the stirring time is 20-30 min.

Further, the filling of the invention is performed by adopting an automatic filling line.

Compared with the prior art, the invention has the following beneficial effects:

1. in the pheromone composition, the component A can stimulate the oestrus of the sows, and meanwhile, the component B with a vegetal source is added in a matching manner, so that the pheromone composition is beneficial to improving the physical and mental states of the sows, plays a role in synergism, can promote the oestrus behavior of the sows and improves the effective oestrus rate.

2. The coloring agent is added into the pheromone composition, so that the coloring agent is combined with skin protein and is marked by color, thereby being beneficial to improving the estrus identification efficiency of workers and simultaneously ensuring the full utilization of pheromones.

3. The pheromone composition preparation is not added with an aromatic heterocyclic compound and other preservatives, and the oxidizable androstenol is protected by filling nitrogen during production and filling, so that the stability of the preparation is improved, and the pheromone composition preparation is safer for animals and human beings.

Detailed Description

The technical solution of the present invention is further described and illustrated by the following specific examples. The raw materials used in the examples of the present invention are those commonly used in the art, and the methods used in the examples are those conventional in the art, unless otherwise specified. It should be understood that the specific embodiments described herein are merely to aid in the understanding of the invention and are not intended to limit the invention specifically.

Example 1

Example 1 provides a pheromone composition spray wherein the amounts of each component are:

androstenone: 120mg of

Androstenol: 12mg of

3-methylindole: 120mg of

Palmitic acid: 1g

Oleic acid: 1g

Linoleic acid: 1g

Bright blue: 1g of a compound;

the information composition spray of this example was prepared by the following method:

weighing 160g of ethanol and isopropanol respectively, adding the ethanol and the isopropanol into a batching tank, uniformly mixing, sequentially adding androstenone, androstenol, 3-methylindole, palmitic acid, oleic acid and brilliant blue according to a ratio, stirring at a stirring speed of 200rpm for 25min to completely dissolve the materials, adding 600g of purified water, and continuously stirring at a speed of 200rpm for 25min to obtain a uniformly mixed semi-finished product solution for later use; filling nitrogen into the filling bottle, driving off oxygen in the bottle, filling the prepared semi-finished product solution into the bottle, controlling the amount of each bottle to be 100 +/-3 ml, and covering and sealing the bottle to obtain the finished pheromone composition spray.

Wherein, androstenone (98.00% or more) and androstenol (98.00% or more) are purchased from Sigma-Aldrich company, 3-methylindole (98.00%), palmitic acid (99%), and oleic acid (90%) are purchased from Sigma-Aldrich company; ethanol (95%, AR grade), isopropanol (99%, pharmaceutical grade) were purchased from Nanjing chemical reagents GmbH; brilliant blue (food grade) is purchased from Shanghai dye research institute, Inc., and purified water is self-made by Ningbo Sansheng biology, Inc. by a reverse osmosis method.

Example 2

In the pheromone composition spray provided in example 2, the amount of each component in 1000mL of the spray is as follows:

androstenone: 120mg of

Androstenol: 12mg of

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Bright blue: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of the embodiment 2 are the same as those of the embodiment 1, and the details are not repeated; among them, geraniol (98%), and angelaldehyde (96%) were purchased from Sigma-Aldrich.

Example 3

In the pheromone composition spray provided in example 3, the amount of each component in 1000mL of the spray is as follows:

androstenone: 120mg of

Androstenol: 12mg of

Myristic acid: 120mg of

Lauric acid: 1g

Linoleic acid: 1g

Bright blue: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of the embodiment 3 are the same as those of the embodiment 1, and the details are not repeated; among them, myristic acid (98%), and lauric acid (98%) were purchased from Nanjing chemical reagents, Inc.

Example 4

In the pheromone composition spray provided in example 4, the amount of each component in 1000mL of the spray is as follows:

androstenone: 150mg of

Androstenol: 10mg of

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Red yeast rice red: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of the embodiment 4 are the same as those of the embodiment 2, and the details are not repeated; wherein the monascus red is purchased from Shanghai dye research institute, Inc.

Example 5

In the pheromone composition spray provided in example 5, the amount of each component in 1000mL of the spray is as follows:

androstenone: 100mg of

Androstenol: 10mg of

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Curcumin: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of the embodiment 5 are the same as those of the embodiment 2, and the details are not repeated; curcumin was purchased from Shanghai dye research institute, Inc., among others.

Example 6

In the pheromone composition spray provided in example 6, the amount of each component in 1000mL of the spray is as follows:

androstenone: 80mg of

Androstenol: 10mg of

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Green grass green: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of example 6 are the same as those of example 2, and the details are not repeated herein, wherein the grass green is purchased from Shanghai dye research institute, Inc.

Example 7

In the pheromone composition spray provided in example 7, the amount of each component in 1000mL of the spray is as follows:

androstenone: 12mg of

Androstenol: 120mg of

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Bright blue: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of example 7 are the same as those of example 2, and the details are not repeated herein.

Example 8

In the pheromone composition spray provided in example 8, the amount of each component in 1000mL of the spray is as follows:

androstenone: 132mg

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Bright blue: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of the embodiment 8 are the same as those of the embodiment 2, and the details are not repeated.

Example 9

In the pheromone composition spray provided in example 9, the amount of each component in 1000mL of the spray is:

androstenol: 132mg

Geraniol: 120mg of

C, ironaldehyde: 520mg of

Bright blue: 1g of a compound;

the solvent and preparation method of the pheromone composition spray of example 9 are the same as those of example 2, and the details are not repeated herein.

Comparative example 1

In the pheromone composition spray provided by comparative example 1, the amount of each component in 1000mL of the spray is as follows:

androstenone: 120mg of

Androstenol: 12mg of

Quinoline: 220mg of

Bright blue: 1g of a compound;

the solvent and the preparation method of the pheromone composition spray of the comparative example 1 are the same as those of the example 1, and the details are not repeated herein.

Comparative example 2

In the pheromone composition spray provided by comparative example 2, the amount of each component in 1000mL of the spray is as follows:

androstenone: 120mg of

Androstenol: 12mg of

Bright blue: 1g of a compound;

the solvent and the preparation method of the pheromone composition spray of the comparative example 2 are the same as those of the example 1, and the details are not repeated herein.

Comparative example 3

The traditional pheromone composition spray product provided by the comparative example 3 is a semi-finished product solution which is uniformly mixed and is prepared by dissolving boar saliva extract 132mg in 1000mL of spray without adding colorant into 95% ethanol for later use; filling nitrogen into the filling bottle, removing oxygen in the bottle, filling the prepared semi-finished product solution into the bottle, controlling the amount of each bottle to be 100 +/-3 ml, and covering and sealing the bottle to obtain the product.

Wherein, the boar saliva extract is prepared by Ningbo Sansheng biology Limited company by a conventional method.

Comparative example 4

Comparative example 4 provides a pheromone composition spray having the same formulation ratio as example 1, which differs from example 1 only in that nitrogen gas charging is not performed before filling, and the remaining steps and conditions are the same as example 1.

Test one:

the effect of the pheromone composition spray was tested in an expansion field of 3000 sows (Du X Long X big) belonging to a certain pig group.

Selecting multiparous sows with the same age, body type, health and other conditions in a gestational house 1-9 and a gestational house 11-14 as test groups, starting to use the pheromone composition spray of the embodiment 1-9 on the third day after the multiparous sows in the gestational house 1-9 are weaned, starting to use the pheromone composition spray of the comparative example 1-4 on the third day after the multiparous sows in the gestational house 11-14 are weaned, and spraying 0.5-1.0 mL each time in the morning and afternoon; the multiparous sows in the gestational house 10 with the age, body type, health and other conditions equivalent to those of the test group are selected as a control group, and the traditional estrus induction is carried out after the multiparous sows in the control group are weaned, and the traditional estrus induction is carried out once every morning and afternoon according to the traditional estrus induction time. Manually checking the condition from the third day, and observing whether the sow has standing performance or not through a back pressing test; and (5) finishing the situation examination on the 10 th day after weaning. The number of sows showing standing performance in different pens was counted, and the oestrus rate of each group was calculated, and the results are shown in table 1. Wherein the oestrus rate (%) < total oestrus head/test head × 100%.

TABLE 1 statistical data on oestrus of sows from examples 1 to 9 and comparative examples 1 to 3

The gestational house 10 uses a traditional boar estrus induction method, and compared with the traditional boar estrus induction method, the sow estrus rate of the gestational house 10 is obviously lower than that of the sows in the gestational house 1-6. In addition, when traditional estrus induction is carried out, the boar and the mouth and the nose of the sow can be in inevitable contact, and particularly, when the boar and the sow are influenced by swine fever epidemic situation, cross contact can be caused, the epidemic situation is diffused, and great biological safety prevention and control pressure is brought to a breeding unit. The pheromone composition takes the artificially synthesized pheromone and the natural plant extract as main components, so that the pheromone composition avoids pathogen carrying and has higher biological safety. Meanwhile, as the coloring agent marks the sow, the workers for checking the emotion do not need to check the ear number repeatedly, the emotion checking operation is simplified, the labor cost of the workers for driving the boars is saved, and great convenience is brought to the expanded production of live pig breeding. The gestational house 13 uses the traditional boar saliva extract for emotion induction, only uses 95% ethanol as a solvent, has strong irritation when being sprayed on the nose, is easy to cause agitation of sows, can also be insensitive to boar odor, has heavy boar saliva extract odor, is difficult to remove when being stuck on clothes of workers, and is easy to cause discomfort of people. The palmitic acid, the oleic acid, the linoleic acid, the geraniol, the angelaldehyde and the like contained in the pheromone composition can sooth the excited emotion of the sow, and the perception of the biological pheromone is effectively improved, so that the oestrus rate is improved.

And (2) test II:

in the breeding of replacement gilts, the sow starts to induce the estrus when the 160-day-old late sow is in the age of 195 days, if the sow has the initial estrus before the age of 195 days, the sow can be considered to have advantages in the breeding performances such as the mating conception rate, the litter size, the healthy litter size and the like, and if the initial estrus does not appear after the age of 285 days, the breeding performance of the sow can be considered to be lower than the average level. Therefore, in this test, replacement gilts (long x white) 833 with equivalent weight, body type and health status from a certain pig group were selected and randomly divided into 4 groups, and estrus induction was performed on replacement gilts (long x white) at the age of 160 days, group a (212) used the pheromone composition spray of example 1, group B (203) used the pheromone composition spray of comparative example 1, group C (210) used the pheromone composition spray of comparative example 3, and group D (208) used the traditional boar estrus induction method, and estrus detection was performed by manual "back pressure test" once every day in the morning and afternoon. And comparing the oestrus conditions of the replacement sows of each group, performing statistical analysis on the initial oestrus rate of 195 days by adopting Chi-square test (Chi 2), taking P <0.05 as a significance judgment standard, performing Fisher exact probability significance analysis on data meeting the Chi 2 test condition, expressing the average initial oestrus day age and the oestrus frequency result before initial mating by using the average value +/-standard error, analyzing the data by using single-factor variance analysis, and analyzing the significance of the data by using a Duncan method.

TABLE 2 examination data of initial condition of replacement gilts

Test grouping	Number of heads	Initial rate of 195 days old	Average age in days of onset	Number of oestrus before initial distribution
					Group A	212	23.2％^c	222.4±15.6^ab	2.0±0.09^a
Group B	203	20.4％^b	207.3±9.7^a	2.2±0.15^b
					Group C	210	15.6％^a	240.6±12.7^b	1.9±0.03^a
Group D	208	20.6％^b	209.0±17.7^a	2.3±0.17^b

Note: the significance of the difference of different test groups in the table is represented by letters

According to the table, the replacement sow of the group A has an initial rate of 195 days old which is significantly higher than that of the rest groups (P <0.05), while the group B has no significant difference in inducing effect with the traditional boar of the group D, while the group C has significantly lower inducing effect with the traditional pheromone composition product than that of the rest groups (P < 0.05). Although the B group can obviously improve the number of oestrus before formulation, the pheromone composition used in the B group contains quinoline, and the pheromone composition can generate great harm to animals and the environment after long-term use. On the basis of androstenone and androstenol, the plant extract is added with the components with the functions of relieving and exciting, so that the anxiety and the stress state of the sow can be relieved, and the oestrus behavior of the sow can be excited. Compared with the existing pheromone product, the pheromone composition disclosed by the invention better meets the market demands of green cultivation and sustainable development, and also has a wider application prospect.

The above embodiments are not exhaustive of the range of parameters of the claimed technical solutions of the present invention and the new technical solutions formed by equivalent replacement of single or multiple technical features in the technical solutions of the embodiments are also within the scope of the claimed technical solutions of the present invention, and if no specific description is given for all the parameters involved in the technical solutions of the present invention, there is no unique combination of the parameters with each other that is not replaceable.

The specific embodiments described herein are merely illustrative of the spirit of the invention and do not limit the scope of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.

Claims

1. The pheromone composition spray is characterized in that the amount of each component in 1000ml of the pheromone composition spray is as follows:

androstenone: 120mg of the total weight of the mixture,

androstenol: 12mg of the total weight of the mixture,

geraniol: 120mg of the total weight of the mixture,

520mg of the ironic aldehyde and the pharmaceutically acceptable carrier,

bright blue: 1g of a compound;

the pheromone composition spray is prepared by the following method:

weighing 160g of ethanol and isopropanol respectively, adding the ethanol and the isopropanol into a mixing tank, uniformly mixing, sequentially adding androstenone, androstenol, geraniol, angelica aldehyde and brilliant blue according to a proportion, stirring at a stirring speed of 200rpm for 25min to completely dissolve the materials, adding 600g of purified water, and continuously stirring at a speed of 200rpm for 25min to obtain a uniformly mixed semi-finished solution for later use; filling nitrogen into the filling bottle, removing oxygen in the bottle, filling the prepared semi-finished product solution into the bottle, controlling the amount of each bottle to be 100 +/-3 ml, and covering and sealing to obtain the pheromone composition spray.