CN112370397B - Application of microcos paniculata leaf extract and preparation thereof in skin care product and skin care product - Google Patents

Application of microcos paniculata leaf extract and preparation thereof in skin care product and skin care product Download PDF

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CN112370397B
CN112370397B CN202011490154.5A CN202011490154A CN112370397B CN 112370397 B CN112370397 B CN 112370397B CN 202011490154 A CN202011490154 A CN 202011490154A CN 112370397 B CN112370397 B CN 112370397B
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microcos paniculata
leaf extract
extract
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CN112370397A (en
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董银卯
张卫红
吕永博
刘月恒
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Nutri Woods Bio Tech Beijing Co ltd
Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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    • A61K8/9789—Magnoliopsida [dicotyledons]
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A61Q1/14—Preparations for removing make-up
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/005—Preparations for sensitive skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

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Abstract

The invention discloses a microcos paniculata leaf extract and application of a preparation of the microcos paniculata leaf extract in skin care products and skin care products, and particularly relates to application of the microcos paniculata leaf extract and a preparation of the microcos paniculata leaf extract in preparation of skin care preparations or skin care products with effects of resisting oxidation and/or aging, resisting inflammatory injury, resisting haze, inhibiting deposition of xanthophyll and/or removing yellow, and skin care products containing the microcos paniculata leaf extract. The microcosmic extraction process is adopted to prepare the microcosmic leaf extract, the total flavone content is high, organic solvent residue is avoided, and the microcosmic leaf extract can be widely applied to preparation of skin care products with the effects of resisting oxidation, resisting inflammatory injury, resisting haze, inhibiting skin tan pigment deposition and removing yellow.

Description

Application of microcos paniculata leaf extract and preparation thereof in skin care product and skin care product
Technical Field
The invention belongs to the field of skin care products, and particularly relates to a microcos paniculata extract and application of a preparation thereof in cosmetics.
Background
In recent years, air pollution becomes a focus of attention of people, and pollutants from various sources, especially atmospheric pollutants such as haze and the like, can not only harm the health of people through breathing to cause respiratory tract and cardiovascular diseases, but also directly or indirectly cause problems of skin allergy, atopic dermatitis, skin aging and the like. Studies have shown that most pollutants cause adverse effects on human skin, such as impaired skin barrier, inflammatory factor production, the appearance of free radicals or other aging-inducing factors, and the like. Since 2011, the terms "haze", "PM2.5", and the like have started to enter the field of view of the public and have attracted a lot of attention throughout society. PM2.5 refers to particulate matters with the diameter less than or equal to 2.5 mu m, and PM2.5 is formally named as 'fine particulate matters' by the national Committee for the examination and approval of scientific and technical terms in 2013. The fine particles are suspended in the air for a long time, which not only causes great damage to systems such as respiration, cardiovascular and the like, but also can cause skin damage such as aging, inflammatory reaction and the like.
The Microcos paniculata leaf is dry leaf of Tiliaceae plant, and is rich in various bioactive components such as flavonoids, alkaloids, triterpenes, organic acids, etc., wherein flavonoids are the main active components. The flavonoids mainly comprise vitexin, isovitexin, narcissus and the like, and have the effects of eliminating free radicals in vivo, enhancing the immunologic function of the human body and the like. At present, the application range of the microcos paniculata extract mainly relates to the fields of beverages, foods, health care medicines and the like, and related researches in the field of cosmetics are few, particularly cosmetic preparations with specific effects. The invention patent CN102727413A discloses a traditional Chinese medicine extracting solution with an anti-skin aging effect, and the preparation method thereof is as follows: soaking the components of rose, safflower, microcos paniculata and peach blossom in water 4-50 times of the components by weight for 10-120 min, and reflux extracting for 30-120 min; collecting the clear liquid of reflux extraction, and concentrating to obtain the Chinese medicinal extractive solution with skin aging resisting effect. However, the application of the microcos paniculata and the extract thereof in cosmetics with the effects of resisting inflammatory injury, resisting haze and the like is not reported in related research/application.
The preparation process of the microcos paniculata extract in the prior art has the problems of low content of active ingredients, residual organic solvent and the like, and influences the application and the full play of skin care effect; and the research on the efficacy of the composition has great limitation, so that the application of the composition in skin care products and/or cosmetics is limited.
Disclosure of Invention
Based on the defects of the prior art, the invention aims to solve the problems of applying the microcos paniculata extract and the preparation thereof to the field of skin care products and providing the skin care products containing the microcos paniculata extract. The skin care product has effects of resisting oxidation, resisting inflammatory injury, resisting haze, inhibiting skin tan pigment deposition, and removing yellow.
The technical scheme of the invention is as follows:
the invention relates to application of a microcos paniculata extract in preparing a skin care preparation and/or a skin care product with antioxidant and/or anti-aging and/or anti-inflammatory injury and/or anti-haze and/or skin fustin deposition inhibiting and/or skin yellowing effects.
According to the invention, the total flavone content in the microcos paniculata leaf extract is 1.04-4.07mg/mL.
According to the invention, the general flavone content in the microcos paniculata extract is 1.95-4.07 mg/mL.
According to the invention, the microcos paniculata leaf extract is prepared by a method comprising the following steps: sieving Microcos paniculata She Cufen, mixing with water, and extracting at certain temperature and pressure to obtain Microcos paniculata leaf extract.
According to the invention, the extraction temperature is between 110 and 135 ℃.
According to the invention, the extraction pressure is between 0.07 and 0.19 MPa.
According to the invention, the extraction time is 20-120 min.
According to some embodiments of the invention, the extraction temperature is 121 ℃ to 135 ℃.
According to some embodiments of the invention, the extraction pressure is between 0.13 and 0.19 MPa.
According to some embodiments of the invention, the extraction time is 30-120min.
According to still further specific embodiments of the present invention, the extraction temperature is 135 ℃.
According to still further specific embodiments of the present invention, the extraction pressure is 0.19 MPa.
According to further specific embodiments of the present invention, the extraction time is 30 min.
According to the invention, the feed-liquid ratio of the microcos paniculata to water is 1:20 (m/m), 1:21 (m/m), 1:22 (m/m), 1:23 (m/m), 1:24 (m/m), 1:25 (m/m), 1:30 (m/m), 1:35 (m/m), 1:40 (m/m), 1:45 (m/m), 1:50 (m/m), 1:55 (m/m), 1:60 (m/m), and point values between the above values.
According to some embodiments of the invention, the feed-liquid ratio of the microcos paniculata to water is 1.
According to still other embodiments of the present invention, the ratio of the microcos paniculata to the water feed is 1:30 (m/m).
According to the invention, the number of extractions is 1-2.
According to the invention, the number of extractions is 2.
According to the invention, the water used is preferably deionized water.
According to the invention, the preparation method of the microcos paniculata leaf extract further comprises the step of sieving coarse powder of microcos paniculata leaves by a sieve with 10-24 meshes (the sieve with 10 meshes below and the sieve with 24 meshes above for later use).
According to the invention, the preparation method of the microcos paniculata extract further comprises a filtration step.
According to the invention, the microcosmic extraction process is adopted to prepare the microcosmic extraction extract.
According to the invention, the microcos paniculata leaf extract is obtained in a high-temperature high-pressure extraction device.
According to the invention, the microcos paniculata extract is carried out in a high-pressure steam cooker.
According to the invention, the total flavone yield of the preparation process of the microcos paniculata extract is 5.37% -8.17%.
According to a second aspect of the invention, the invention provides an application of a liquid preparation in preparing cosmetics with effects of resisting oxidation and/or aging and/or inflammation injury and/or haze and/or inhibiting skin fulvic acid deposition and/or removing yellow.
According to the present invention, the liquid preparation includes a microcos paniculata extract, a liquid dispersant, and a color fixative.
According to the invention, the liquid preparation is prepared by adding 0.2-2.0wt% of activated carbon into the microcos paniculata extract for decolorization, filtering and concentrating, and compounding 10-50wt% of liquid dispersant and color fixative.
According to some embodiments of the present invention, the microcos paniculata extract in the liquid formulation is prepared by a method comprising the steps of: sieving cloth residues She Cufen, mixing with water, and extracting at 110-135 deg.C under 0.07-0.19MPa for 20-120 min.
According to some specific embodiments of the invention, the extraction temperature is between 121 ℃ and 135 ℃.
According to some embodiments of the invention, the extraction pressure is between 0.13 and 0.19 MPa.
According to some embodiments of the invention, the extraction time is 30-120min.
According to still further specific embodiments of the present invention, the extraction temperature is 135 ℃.
According to still further specific embodiments of the present invention, the extraction pressure is 0.19 MPa.
According to further specific embodiments of the present invention, the extraction time is 30 min.
According to the invention, the feed-liquid ratio of the microcos paniculata to water is 1:60 (m/m), for example, may be 1:20 (m/m), 1:21 (m/m), 1:22 (m/m), 1:23 (m/m), 1:24 (m/m), 1:25 (m/m), 1:30 (m/m), 1:35 (m/m), 1:40 (m/m), 1:45 (m/m), 1:50 (m/m), 1:55 (m/m), 1:60 (m/m), and point values between the above values.
According to some embodiments of the invention, the feed-to-liquid ratio of the microcos paniculata to water is 1:20-1:40 (m/m).
According to still other embodiments of the present invention, the ratio of the microcos paniculata to the water feed is 1:30 (m/m).
According to the invention, the number of extractions is 1-2.
According to the invention, the number of extractions is 2.
According to the invention, the water used is preferably deionized water.
According to the invention, the preparation method of the microcos paniculata leaf extract further comprises the step of sieving coarse powder of microcos paniculata leaves by a sieve with 10-24 meshes (the sieve with 10 meshes below and the sieve with 24 meshes above for later use).
According to the invention, the preparation method of the microcos paniculata extract further comprises a filtration step.
According to some embodiments of the invention, the amount of activated carbon added to the liquid formulation is 0.2wt% to 2.0wt% of the microcos paniculata extract, and may be, for example, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1.0wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%, 1.6wt%, 1.7wt%, 1.8wt%, 1.9wt%, 2.0wt%, and points between the foregoing values.
According to still further embodiments of the present invention, the activated carbon is added to the liquid preparation cosmetic in an amount of 0.5wt% based on the extract of microcos paniculata.
According to some embodiments of the invention, the liquid dispersant in the liquid formulation is one or more of glycerol, 1,3 propylene glycol, butylene glycol, and propylene glycol.
According to some embodiments of the invention, the liquid dispersant in the liquid formulation is glycerol.
According to some embodiments of the invention, the liquid dispersant is added in an amount of 10wt% to 50wt% of the microcos paniculata extract in the liquid formulation.
According to still further embodiments of the present invention, the liquid dispersion agent is added to the liquid preparation cosmetic in an amount of 45wt% of the microcos paniculata extract.
According to the present invention, the cosmetic may be a basic cosmetic, a face makeup cosmetic, a head care product, a body makeup cosmetic, etc., and the formulation thereof is not particularly limited and may be appropriately selected according to various purposes.
According to the invention, the cosmetic is facial cleanser, makeup remover, toner, lotion, essence cream, essence oil, skin oil, massage oil, facial cream, eye cream, facial mask, gel and the like, without limitation.
According to a third aspect of the invention, the application of the solid preparation in preparing the skin care product with the effects of resisting oxidation and/or aging and/or inflammation injury and/or haze and/or inhibiting skin fulvic acid deposition and/or removing yellow is provided.
According to the invention, the solid preparation comprises the microcos paniculata extract and a solid dispersing agent.
According to the invention, the solid preparation is prepared by adding activated carbon into the microcos paniculata leaf extract for decolorization, filtering and concentrating, compounding a solid dispersing agent, and performing vacuum freeze drying.
According to some embodiments of the present invention, the solid preparation of the microcos paniculata extract is prepared by a method comprising the following steps: sieving Microcos paniculata She Cufen, mixing with water, extracting at 110-135 deg.C under 0.07-0.19MPa for 20-120 min to obtain Microcos paniculata leaf extract;
according to some embodiments of the invention, the extraction temperature is 121 ℃ to 135 ℃.
According to some embodiments of the invention, the extraction pressure is between 0.13 and 0.19 MPa.
According to some embodiments of the invention, the extraction time is 30-120min.
According to still further specific embodiments of the present invention, the extraction temperature is 135 ℃.
According to still further specific embodiments of the present invention, the extraction pressure is 0.19 MPa.
According to further specific embodiments of the present invention, the extraction time is 30 min.
According to the invention, the feed-liquid ratio of the microcos paniculata to water is 1:60 (m/m), for example, may be 1:20 (m/m), 1:21 (m/m), 1:22 (m/m), 1:23 (m/m), 1:24 (m/m), 1:25 (m/m), 1:30 (m/m), 1:35 (m/m), 1:40 (m/m), 1:45 (m/m), 1:50 (m/m), 1:55 (m/m), 1:60 (m/m), and point values between the above values.
According to some embodiments of the invention, the feed-liquid ratio of the microcos paniculata to water is 1:40 (m/m).
According to still other embodiments of the present invention, the ratio of the microcos paniculata to the water feed is 1:30 (m/m).
According to the invention, the number of extractions is 1-2.
According to the invention, the number of extractions is 2.
According to the invention, the water used is preferably deionized water.
According to the invention, the preparation method of the microcos paniculata leaf extract further comprises the step of sieving coarse powder of microcos paniculata leaves by a sieve with 10-24 meshes (the sieve with 10 meshes below and the sieve with 24 meshes above for later use).
According to the invention, the preparation method of the microcos paniculata extract further comprises a filtration step.
According to some embodiments of the invention, the amount of activated carbon added to the solid formulation is 0.2wt% to 2.0wt% of the microcos paniculata extract, and may be, for example, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1.0wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%, 1.6wt%, 1.7wt%, 1.8wt%, 1.9wt%, 2.0wt%, and points therebetween.
According to still other embodiments of the present invention, the amount of activated carbon added to the solid preparation is 0.5wt% of the microcos paniculata extract.
According to some embodiments of the invention, the solid dispersant in the solid preparation is one or more of chitosan, trehalose, beta-cyclodextrin and polyvinylpyrrolidone (PVP k 30).
According to some embodiments of the invention, the solid dispersant in the solid formulation is beta-cyclodextrin.
According to still further embodiments of the present invention, the solid dispersion agent is added to the solid preparation in an amount of 20wt% of the Microcos paniculata extract.
According to the present invention, the cosmetic may be a basic cosmetic, a face makeup cosmetic, a head care product, a body makeup cosmetic, etc., and the formulation thereof is not particularly limited and may be appropriately selected according to various purposes.
According to the invention, the cosmetic is facial cleanser, makeup remover, toner, lotion, essence cream, essence oil, skin oil, massage oil, facial cream, eye cream, facial mask, gel and the like, without limitation.
In a fourth aspect, the present invention provides a cosmetic comprising, as an active ingredient, an extract of microcos paniculata according to the first aspect of the present invention and/or a liquid preparation according to the second aspect and/or a solid preparation according to the third aspect, and a process for producing the same.
According to the invention, the cosmetic contains the microcos paniculata leaf extract and auxiliary materials which are conventional in the field of cosmetics.
According to the invention, the cosmetic contains liquid preparations and adjuvants customary in the cosmetic field.
According to the invention, the cosmetic contains solid preparations and adjuvants customary in the cosmetic field.
According to some embodiments of the invention, the emulsion comprises the following components in parts by weight:
phase A: cetearyl alcohol/coco glucoside 1.5% -5.0%, pentaerythritol distearate 1.0% -5.0%, stearic acid 1.0% -3.0%, cetearyl alcohol 0.1% -2.0%;
phase B: 0.01-0.5% of xanthan gum, 1.0-10.0% of butanediol, 2.0-8.0% of glycerol, 0.03-0.1% of EDTA disodium and water TO100;
and C phase: sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80.1% -0.5%;
phase D: 2.0 to 10.0 percent of microcos paniculata leaf extract;
e phase: 0.5 to 1.0 percent of phenoxyethanol/caprylyl glycol/decanediol.
According to the invention, the content of the flavone in the microcos paniculata extract in the emulsion is 1.04-4.07mg/mL.
According to the present invention, the emulsion may be prepared by a method conventional in the art.
According to some embodiments of the invention, the emulsion is prepared by a method comprising the steps of:
1) Weighing the phase A raw material, adding the phase A raw material into an oil phase tank, heating to 80-85 ℃, and keeping the temperature for 0.5-1 hour;
2) Pre-dispersing xanthan gum in phase B with glycerol and butanediol, adding into a pot, adding other materials in phase B, heating to 80-85 deg.C, and maintaining the temperature for 0.5-1 hr;
3) Starting homogenizing, adding the phase A raw material into the phase B raw material while homogenizing, and homogenizing for about 3-10 minutes;
4) Stirring for 0.5-1 hour under heat preservation, and cooling;
5) When the temperature is reduced to 70 ℃, adding the pre-dispersed phase C and phase D, and homogenizing for 3-10 minutes;
6) When the temperature reaches 40 ℃, adding the E-phase raw material, continuing stirring and discharging.
According to some embodiments of the invention, the essence consists of the following components in parts by weight:
phase A: 0.2 TO 0.5 percent of carbomer, 0.03 TO 0.1 percent of EDTA disodium, 1.0 TO 10.0 percent of butanediol, 0.01 TO 1.0 percent of allantoin and water TO100;
phase B: 0.5 to 1.0 percent of sodium hydroxide;
and C phase: 0.5 to 1.0 percent of microcos paniculata leaf extract;
phase D: 0.5 to 1.0 percent of phenoxyethanol/caprylyl glycol/decanediol;
according to the invention, the flavone content in the microcos paniculata leaf extract in the Jinghua is 1.04-4.07mg/mL.
According to the present invention, the essence may be prepared by a conventional method in the art.
According to some embodiments of the invention, the essence is prepared by a method comprising the following steps:
1) Dispersing carbomer in water, swelling uniformly, adding phase A other raw materials, stirring, and heating to 80-85 deg.C;
2) Stirring and cooling to 50-60 ℃, adding the phase B raw material for neutralization, and stirring until the phase B raw material is completely dissolved;
3) Cooling to 40-45 deg.C under stirring, adding D phase raw material, stirring, and cooling;
4) Sampling and detecting at the temperature below 38 ℃;
5) Aging for 24 hours;
6) And (6) discharging.
According to the invention, the cosmetic has antioxidant and/or anti-aging and/or anti-inflammatory damage and/or anti-haze and/or anti-fulvic element deposition and/or anti-yellowing effects.
The invention has the beneficial effects that:
the microcosmic extraction process is adopted to prepare the microcosmic extraction product, and the microcosmic extraction product is extracted from the microcosmic extraction product to obtain the microcosmic extraction product. The folium Microcoris Paniculatae extract can be widely used in preparation of skin care preparation or skin care product with effects of scavenging free radicals, resisting oxidation, resisting inflammatory injury, resisting haze, inhibiting tawny pigment deposition, and removing yellow.
Drawings
FIG. 1 is a graph showing the results of the inhibition of non-enzymatic glycosylation of proteins by the extract of Microcos paniculata;
FIG. 2 is a graph showing the effect of liquid preparation of Microcos paniculata on the survival rate of THP-1 cells;
FIG. 3 is a graph showing the effect of the liquid preparation of Microcos paniculata on the amount of IL-1 β secretion, which is a cytokine of THP-1.
Detailed Description
The invention is further illustrated below with reference to specific examples, to which, however, the invention is not restricted.
It should be appreciated by those skilled in the art that the present invention is not limited to the above embodiments, and any changes and modifications to the present invention are within the scope of the present invention.
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the experimental materials and reagents are commercially available, unless otherwise specified.
In the following examples, the starting materials used were: the leaf of panicum fruit was purchased from Junyutang medicine station in Bozhou city.
The apparatus used in the present invention is shown in table 1.
Name (R) Model number Suppliers of goods
Vertical high-pressure steam sterilizing pot LDZM-30L-II Shanghai Shen An autoclave
Vacuum freeze drier LGJ-30FD Beijing Songyuan Huaxing science and technology development company
TABLE 1 test apparatus
1. Screening of extraction solvent
The method comprises the steps of taking the cloth residue leaves which are crushed and sieved by a 10-24-mesh sieve (below the 10-mesh sieve and above a 24-mesh sieve for later use) as raw materials, selecting numerical control ultrasonic extraction equipment, fixing ultrasonic frequency at 40 kHZ, temperature at 45 ℃, ultrasonic power at 400W, ultrasonic treatment for 30min, and selecting an extraction solvent for extracting the flavone in the cloth residue leaves by taking pure water, 95% ethanol, acetone and ethyl acetate as extraction solvents, wherein the material-liquid ratio is 1.
TABLE 2 comparison of different extraction solvents
Solvent(s) Total Flavonoids yield (%) Total Flavonoids content (mg/mL)
Pure water 5.67 2.84
95% ethanol 6.11 3.06
Acetone (II) 4.27 2.14
Ethyl acetate 3.99 2.00
As can be seen from Table 2, the total flavone yield of the microcos paniculata leaves extracted by different solvents is the best in ethanol, the second in pure water and the worst in propanol and ethyl acetate. In combination with the production safety requirement, the ethanol dosage is large, the problems of explosion, organic solvent residue, skin irritation and the like are easily caused, and the water is non-toxic, safe and easy to recycle, so that the subsequent experiments all use pure water as an extraction solvent.
2. Total flavone testing method
Using NaNO 2 -Al(NO 3 ) 3 And (3) testing the yield of the total flavone in the folium Microcoris Paniculatae extract by a colorimetric method (reference standard: a spectrophotometric colorimetric method for determining the content of the total flavone in the propolis in GB/T20574-2006).
Examples 1 to 4
Sieving the coarse powder of the microcos paniculata leaves with a 10-24 mesh sieve (below the 10 mesh sieve and above the 24 mesh sieve for later use), adding deionized water according to the proportion of 1. Examples 1-4 micropressure preparation methods specific feed-liquid ratio (m/m), extraction time (min), extraction temperature (. Degree. C.), extraction pressure (MPa) and extraction times are shown in Table 3.
TABLE 3 optimization of the feed-liquid ratio parameters for micropressure preparation process
Figure DEST_PATH_IMAGE001
Examples 5 to 8
Sieving the coarse powder of the microcos paniculata leaf with a 10-24 mesh sieve (below the 10 mesh sieve and above the 24 mesh sieve for later use), adding deionized water according to the proportion of 1. Examples 5-8 micropressure preparation Process the specific feed-to-liquid ratio (m/m), extraction time (min), extraction temperature (. Degree. C.), extraction pressure (MPa) and the number of extractions are shown in Table 4.
TABLE 4 micropressure preparation Process extraction time parameter optimization
Figure 633400DEST_PATH_IMAGE002
Examples 9 to 12
Sieving the coarse powder of the microcos paniculata leaves with a 10-24 mesh sieve (the part below the 10 mesh sieve is on the 24 mesh sieve for standby), adding deionized water according to the proportion of 1 to 30 (m/m), extracting for 30min for 2 times at the temperature of 110-135 ℃ and under the pressure of 0.07-0.19MPa, combining the filtrates, and filtering to obtain the microcos paniculata leaf extract. Examples 9-12 micropressure preparation methods the specific feed-to-liquid ratios (m/m), extraction times (min), extraction temperatures (. Degree. C.), extraction pressures (MPa) and extraction times are shown in Table 5.
TABLE 5 micropressure preparation Process extraction temperature and pressure parameter optimization
Figure 147558DEST_PATH_IMAGE004
Comparative example 1 (atmospheric cooking technique)
Sieving coarse powder of cloth residue leaves with a 10-24 mesh sieve (below the 10 mesh sieve and above the 24 mesh sieve for later use), and mixing the powder with the powder according to the weight ratio of 1:20 Adding deionized water at a ratio of (m/m), steaming at 95-100 deg.C under normal pressure for 2 times, each for 30min, mixing filtrates, and filtering to obtain folium Microcoris Paniculatae extract.
Comparative example 2 (ultrasonic assisted extraction method)
Sieving the coarse powder of the microcos paniculata leaves with a 10-24-mesh sieve (the sieve below the 10-mesh sieve is on the sieve with 24 meshes for later use), and mixing the coarse powder of the microcos paniculata leaves with the powder according to a ratio of 1:20 Adding deionized water at a ratio of (m/m), setting power at 400W and temperature at 70-75 deg.C, ultrasonic-assisted extracting for 2 times, each for 30min, mixing filtrates, and filtering to obtain folium Microcoris Paniculatae extract.
Comparative example 3
Sieving the coarse powder of the microcos paniculata leaves with a 10-24-mesh sieve (the sieve below the 10-mesh sieve is on the sieve with 24 meshes for later use), and mixing the coarse powder of the microcos paniculata leaves with the powder according to a ratio of 1:20 Adding deionized water at a ratio of (m/m), setting the technological parameters of comparative example 3, extracting for 30min at 105 deg.C under 0.03MPa for 1 time, and filtering to obtain folium Microcoris Paniculatae extract.
The experimental results are as follows:
taking the raw material microcos paniculata, taking water as an extraction solvent, extracting by the processes of examples and comparative examples, and testing the yield and the content of total flavonoids in microcos paniculata in the extract, wherein the test results are shown in Table 6:
TABLE 6 Total flavone yield and content in Bupleura leaf extract
Serial number Total Flavonoids yield (%) Total Flavonoids content (mg/mL)
Example 1 5.37 2.69
Example 2 5.86 1.95
Example 3 6.12 1.53
Example 4 6.22 1.04
Example 5 6.09 2.03
Example 6 6.33 2.11
Example 7 6.18 2.06
Example 8 6.21 2.07
Example 9 8.13 4.07
Example 10 7.52 2.51
Example 11 8.12 2.71
Example 12 8.17 2.72
Comparative example 1 5.32 2.66
Comparative example 2 5.98 2.99
Comparative example 3 4.73 2.37
According to the test results of the buchana leaf flavones in the examples 10-12, the total flavone yield is gradually increased along with the increase of the extraction temperature and pressure of the micro-pressure extraction process, and when the extraction temperature reaches 128 ℃, the extraction temperature is continuously increased, so that the buchana leaf flavone yield is basically kept unchanged; on the other hand, the increase of the extraction temperature leads to the increase of the compressive strength and safety risk factor of the equipment, so that the maximum temperature of the micro-pressure extraction process is recommended to be 135 ℃, and the corresponding pressure is 0.19 MPa.
Taking the yield and the content of the total flavone as research targets, adopting a micro-pressure extraction process for preparation, investigating the influence of different material-liquid ratios (m/m), extraction time (min), extraction temperature (DEG C) and corresponding pressure (MPa) on the yield of the target total flavone, and optimizing the better preparation process as follows: the feed-liquid ratio is 1.
The total flavone yield of the microcosmic extraction process for preparing the microcosmic extraction is high, the residue of organic solvent is avoided, and the extraction time is shortened.
Example 13 (liquid preparation)
The microcos paniculata leaf extract obtained in example 12 was decolorized by adding 0.5wt% of activated carbon, filtered and concentrated, and mixed with 45wt% of liquid dispersant glycerol and 0.12 wt% of disodium EDTA as a color fixative to obtain a microcos paniculata leaf extract liquid preparation of the present invention.
Example 14 (solid preparation)
The microcos paniculata leaf extract obtained in example 12 was added with 0.5wt% of activated carbon for decolorization, filtered and concentrated, and then compounded with 20wt% of a solid dispersant, i.e., beta-cyclodextrin, and vacuum freeze-dried to obtain the solid preparation of the present invention.
Comparative example 4 (liquid preparation)
And (4) adding 0.5wt% of activated carbon into the microcos paniculata extract obtained in the comparative example 3 for decolorization, filtering and concentrating, and compounding 45wt% of liquid dispersant glycerol and 0.12 wt% of disodium EDTA to obtain the comparative example 4.
COMPARATIVE EXAMPLE 5 (solid preparation)
And (3) adding 0.5wt% of activated carbon into the microcos paniculata leaf extract obtained in the comparative example 3 for decolorization, filtering and concentrating, compounding 20wt% of solid dispersant beta-cyclodextrin, and performing vacuum freeze drying to obtain the comparative example 5.
Example 15 (emulsion)
Figure 932980DEST_PATH_IMAGE006
Example 16 (emulsion)
Figure 481773DEST_PATH_IMAGE008
Example 17 (emulsion)
Figure 897711DEST_PATH_IMAGE010
Examples 15-17 the emulsion preparation process included the following steps:
1) Weighing the phase A raw material, adding the phase A raw material into an oil phase tank, heating to 80-85 ℃, and keeping the temperature for 0.5-1 hour;
2) Pre-dispersing xanthan gum in phase B with glycerol and butanediol, adding into a pot, adding other materials of phase B, heating to 80-85 deg.C, and maintaining the temperature for 0.5-1 hr;
3) Starting homogenizing, adding the phase A raw material into the phase B raw material while homogenizing, and homogenizing for about 3-10 minutes;
4) Stirring for 0.5-1 hour under heat preservation, and cooling;
5) When the temperature is reduced to 70 ℃, adding the pre-dispersed phase C and phase D, and homogenizing for 3-10 minutes;
6) When the temperature reaches 40 ℃, adding the E-phase raw material, continuing stirring and discharging.
Examples 18 to 20
Figure 961482DEST_PATH_IMAGE012
Test example 1: scavenging free radicals
Scavenging ABTS free radicals is a routine way to analyze antioxidants. The clearance rate in the test is used for expressing the capability of the tested substance for eliminating free radicals, and the higher the clearance rate is, the stronger the oxidation resistance is. The specific test method comprises the following steps:
0.0192 g of ABTS is added into 5.00 mL deionized water to be dissolved and mixed evenly to prepare 7 mmol.L -1 ABTS stock solution of (a). Weighing 0.1892 g potassium persulfate, adding 5.000 mL deionized water for dissolving, mixing well to prepare 140 mmol.L -1 The stock solution of (1). A certain amount of 7 mmol. Multidot.L -1 ABTS and 140 mmol. Multidot.L of -1 Mixing with potassium persulfate, and standing at room temperature in the dark for a period of time to form ABTS free radical stock solution. A certain amount of ABTS free radical stock solution is taken and diluted by 50% ethanol, so that the light absorption value of the final test negative control is 0.70 +/-0.02.
Diluting or dissolving the test solution 1 in 50% ethanol to obtain test solution 1 with mass concentration of 0.5%, 0.20%, 0.10% and 0.05%; example 14 was dissolved in 50% ethanol to obtain test solution 2 having mass concentrations of 0.10%, 0.05%, 0.02%, and 0.01%.
Adding the test solution 1 or the test solution 2 and the ABTS free radical stock solution in sequence, uniformly mixing by vortex, placing in an enzyme-labeling instrument for incubation in dark, and measuring the light absorption value of the enzyme-labeling instrument at the wavelength of 734 nm at intervals of 20min. ABTS free radical clearance was calculated according to equation (1).
Figure DEST_PATH_IMAGE013
In formula (1): a is OD value of the test solution 1 or the test solution 2 mixed with the ABTS solution, B is OD value of the 50% ethanol mixed with the ABTS solution, and C is OD value of the 50% ethanol mixed with the test solution.
The antioxidant activity of the samples of the microcos paniculata extract (liquid formulation example 13, solid formulation example 14) was evaluated by ABTS free radical test, and the test results are shown in tables 7 and 8.
TABLE 7 Eleocharis paniculata leaf extract in example 13 for ABTS radical scavenging
Test concentration (%) ABTS radical scavenging ratio (%)
0.50 91
0.20 68
0.10 41
0.05 19
TABLE 8 Eleocharis paniculata leaf extract in example 14 exhibits ABTS radical scavenging efficiency
Test concentration (%) ABTS radical scavenging ratio (%)
0.10 100
0.05 100
0.02 66
0.01 37
As can be seen from the results in tables 7 and 8, the microcos paniculata leaf extract prepared by the invention has the efficacy of eliminating ABTS free radical activity, and shows that the microcos paniculata leaf extract has the capacity of resisting oxidative damage at a biochemical level.
Test example 2: anti-inflammatory injury
Lipopolysaccharide (LPS) is adopted for induction, an action object is RAW264.7, general body inflammation is simulated, and a tested inflammatory factor is IL-1 beta. IL-1 beta plays an important role in immune regulation and inflammatory reaction processes, is considered to be one of main endogenous mediators causing fever reaction in the inflammatory reaction process, and once the content of inflammatory factors in skin cells is increased and the skin temperature is higher than normal temperature, the skin inflammatory reaction is aggravated and the body inflammatory cascade reaction is initiated.
An anti-inflammatory assay (IL-1 beta) assay based on the LPS-induced RAW264.7 cell inflammation model:
(1) Inoculation: seeding the cells into 96-well plates, 37 ℃,5% CO 2 Incubating in an incubator overnight;
(2) Preparing liquid: test substances and positive controls were prepared according to table 9;
(3) Administration: the experimental groups and concentration settings according to table 9 were administered in groups of 3 duplicate wells per treatment group after 24h of growth of the cell plating in the 96-well plate. In group 1, cell culture medium containing 1 ‰ DMSO was added to both blank control and negative control, and added to positive controlCell culture medium containing 0.001% dexamethasone; in group 2, the blank control and the negative control were added with cell culture medium, and the sample group was added with cell culture medium containing samples at corresponding concentrations at 37 ℃ with 5% CO 2 The incubator continues to culture for 24h.
Table 9 experimental groupings and concentration settings
Figure DEST_PATH_IMAGE015
(4) And (3) LPS induction: after culturing for 24h, sucking out the culture medium in the plate, adding PBS for light washing once, adding cell culture medium into a blank control group, adding cell culture medium containing LPS into a negative control group, a sample group and a positive control group, and culturing at 37 ℃ with 5% CO 2 The incubator continues to culture for 24h and then collects the supernatant.
(5) Detecting the content of IL-1 beta: the cell supernatants of the wells were collected and assayed for the content of the cytokine IL-1. Beta. According to the ELISA kit protocol, the results are shown in Table 10.
TABLE 10 detection of inflammatory factor IL-1 beta expression
Figure DEST_PATH_IMAGE017
Note: the lowest limit of quantitation (the lowest concentration of the standard curve) of the method is 12.5 pg/mL, and the lowest detection limit is 1pg/mL.
Compared with the LPS uninduced (LPS-) group, the LPS induction (LPS +) causes the expression quantity of the inflammatory factor IL-1 beta to be remarkably increased (P < 0.01), which indicates that the LPS induction modeling is successful; compared with SC (0.1% DMSO, LPS +), dexamethasone in the PC (dexamethasone) group can significantly reduce the expression amount of IL-1 beta (P < 0.01) at the administration concentration of 0.001%, which indicates that the detection result is effective.
Compared with NC (LPS +) group, the solid preparation of the microcos paniculata extract (example 14) can obviously reduce the content of IL-1 beta factor at the concentration of 0.001%, and the inhibition effect is equivalent to that of a positive control (0.001% dexamethasone) (Table 10), and compared with comparative example 5, the anti-inflammatory effect is more obvious.
Test example 3: inhibiting fulvestrant deposition
One of the main features of skin aging is skin yellowing and the appearance of age spots, mainly due to lipid peroxidation and non-enzymatic glycosylation reactions. The non-enzymatic glycosylation reaction in organisms refers to that under the condition of no enzyme catalysis, the aldehyde group or ketone group of reducing sugar and the amino group of macromolecules such as protein and the like undergo Maillard (Maillard) reaction to generate yellow brown glycosylation end products (AGEs).
By inhibiting the nonenzymatic glycosylation of proteins, the inhibitory effect of substances on the deposition of brown pigment can be reflected. The samples to be tested were: microcos paniculata extract obtained in example 12, microcos paniculata extract obtained in comparative example 3, and a positive control (Vc ethyl ether) used in the experimental model were obtained. The specific test method is as follows:
mixing a 0.5mol/L bovine serum albumin solution (BCA) and a 20 mg/mL fructose solution in equal volume to obtain a BCA-fructose reaction solution. The reagents were added sequentially in the order of Table 11, and after addition, vortexed and mixed. After incubation for 5 days at 37 ℃ in the absence of light, the fluorescence intensity was measured at an excitation wavelength of 370 nm and an emission wavelength of 440 nm.
And (3) calculating the inhibition degree of the sample to be detected on the non-enzymatic glycosylation reaction according to the formula (2) according to the Fluorescence intensity (RFU, relative Fluorescence Unit).
Figure 753857DEST_PATH_IMAGE018
TABLE 11 grouping and addition of non-enzymatic glycosylation test experiments
Group of Sample to be tested (μ L) PBS buffer (mu L) BCA-fructose reaction solution (mu L)
Group to be tested 200 0 200
Negative control 0 200 200
Blank group 200 200 0
The results of the non-enzymatic glycosylation test are shown in fig. 1, the microcos paniculata extract prepared in example 12 has excellent effect of inhibiting the non-enzymatic glycosylation of protein, and the effect of the non-enzymatic glycosylation inhibiting rate of protein is better than that of Vc ethyl ether (positive control) under the same adding amount (1%) and that of comparative example 3 under the same concentration, namely the microcos paniculata extract is better than that of Vc ethyl ether and comparative example 3 in the aspect of inhibiting the yellow-brown spots.
Test example 4: haze resistance test
In recent years, the frequent appearance of 'haze' weather in China makes people pay more attention to the health hazards of the environment and the environmental changes to human bodies. However, suspended Particulate Matter (PMs) in the air is one of the important factors causing air pollution, and seriously affects human health. PMs are a complex mixture containing metals, minerals, organic toxins and microorganisms.
The skin provides an effective biological barrier function for the external environment and the body to resist important tissues and organs of environmental pollution, and researches show that air pollution can cause oxidative stress and inflammation of the skin, cause the barrier function of the skin to be degraded, and cause skin diseases (such as erythema, edema, hyperplasia, skin aging, allergic dermatitis, psoriasis and even carcinogenesis) when the haze content exceeds the protective potential and the skin structure is damaged.
PM2.5 is adopted for induction, the effect is human monocyte HTP-1, and the influence of haze on the IL-1 beta secretion amount in the skin is inspected.
(1) THP-1 cell-based safety concentration detection
This time, the sample is set to 6 concentrations, each concentration is set to 3 repeat wells, and simultaneously, the test is set to blank control wells. The test adopts a CCK-8 detection method to screen the maximum safe concentration of cell drug delivery. The specific operation steps are as follows:
a. inoculation: inoculating THP-1 cells in logarithmic phase to a 96-well plate, and adding 100 mu L of THP-1 cells into each well;
b. adding medicine: according to the concentration setting of table 12, test substances with different concentrations are prepared, then the test substances with different concentrations are added, and the mixture is lightly blown and beaten uniformly. The plates were placed at 37 ℃ in 5% CO 2 The incubator incubates 24h.
TABLE 12 sample cytotoxic concentration settings
Sample name Sample concentration (w/v,%)
Example 13 0.00125、0.0025、0.005、0.01、0.02、0.04
Comparative example 4 0.00125、0.0025、0.005、0.01、0.02、0.04
c. And (3) detection: after culturing the cells at 24h, 10. Mu.L of CCK-8 was added to each well, mixed gently, and incubated at 37 ℃ for 1h in the dark. After incubation, the OD was read with a microplate reader 450nm The value is obtained.
d. Calculating the formula:
Figure DEST_PATH_IMAGE019
as can be seen from fig. 2, the survival rate of THP-1 cells of the liquid preparation of buchner leaf prepared in example 13 was ensured to be 85% or more, and it was found that: the maximum safe administration concentration of the liquid preparation of buchner leaf in example 13 was determined to be 0.04%, based on the maximum safe administration concentration test, and the effect of PM2.5 on the secretion amount of THP-1 cytokines 1L-1 β was tested by ELISA.
(2) Assay for the amount of IL-1 beta secretion based on the induction of THP-1 cytokine by PM2.5
a, inoculation: inoculating cells in logarithmic phase to a 96-well plate, and adding 100 mu L of cells into each well;
b, adding medicine: according to the concentration setting in table 13, test substances with different concentrations were prepared, and then test substances with different concentrations were added and blown gently and beaten uniformly. The plates were placed at 37 ℃ in 5% CO 2 The incubator incubates and cultures 4h.
c, adding a stimulant: adding 1640 culture medium into blank control group, adding PM into negative control group and sample group 2.5 37 ℃ 5% of the cell culture medium of (1) 2 The incubator continues to culture 24h and then collects the supernatant.
d, detecting the content of IL-1 beta: and transferring the cells of each hole to a 1.5 mL clean EP tube, centrifuging to obtain a supernatant, and detecting the content of the IL-1 beta of the cell inflammatory factor according to the operation instruction of the ELISA kit.
e, data processing: each item of data obtained in the experiment was processed and plotted by Excel software. Statistical analysis was performed using SPSS 17.0, and the differences were judged to be significant when p <0.05 using one-way analysis of variance (ANOVA) for group comparisons.
Table 13 test groupings and concentration settings
Figure 750632DEST_PATH_IMAGE021
The results of the haze-resistant test (shown in fig. 3) show that the liquid preparation prepared in example 13 has excellent effect of inhibiting the secretion of the THP-1 cytokine IL-1 β caused by the PM2.5+ stimulus, and the effect is superior to that of comparative example 4, which indicates that the microcos paniculata extract of the present invention has a skin-protecting effect superior to that of comparative example 4 on the haze-resistant effect cosmetic.

Claims (9)

1. Application of folium Microcoris Paniculatae extract in preparing skin care preparation with anti-inflammatory injury and/or anti-haze and/or anti-yellow effects;
the microcos paniculata leaf extract is prepared by a preparation method comprising the following steps: sieving the cloth residues She Cufen, mixing with water according to a material-liquid ratio of 1.
2. The application of claim 1, wherein in the preparation method, the feed-liquid ratio of the microcos paniculata to water is 1-20-1 (m/m), the extraction temperature is 121-135 ℃, the extraction pressure is 0.13-0.19MPa, and the extraction time is 30-120min.
3. The use of claim 1, wherein the preparation method comprises the following steps of 1-30 (m/m) feed-liquid ratio of the microcos paniculata to water, 135 ℃ extraction temperature, 0.19MPa pressure and 30min extraction time.
4. The application of a liquid preparation in preparing a skin care preparation with anti-inflammatory injury and/or anti-haze and/or yellow-dispelling effects is characterized in that the liquid preparation is prepared by adding 0.2-2.0wt% of activated carbon into a microcos paniculata leaf extract for decolorization, filtering and concentrating, and compounding 10-50wt% of a liquid dispersant and a color fixative, wherein the liquid dispersant is added into the microcos paniculata leaf extract;
the microcos paniculata leaf extract is obtained by a preparation method comprising the following steps: sieving the cloth residues She Cufen, mixing with water according to the material-liquid ratio of 1;
the liquid dispersant is one or more of glycerol, 1,3 propanediol, butanediol or propanediol;
the color fixative is one or more of EDTA disodium, citric acid, beta-cyclodextrin, tartaric acid or Vc ethyl ether.
5. The application of a solid preparation in preparing a skin care preparation with anti-inflammatory injury and/or anti-haze and/or anti-yellowing effects is characterized in that the solid preparation is prepared by adding 0.2-2.0wt% of activated carbon in a microcos paniculata extract for decolorization, filtering and concentrating, compounding and adding 10-50wt% of solid dispersing agent in the microcos paniculata extract, and performing vacuum freeze drying;
the solid dispersing agent is one or more of chitosan, trehalose, beta-cyclodextrin or polyvinylpyrrolidone;
the microcos paniculata leaf extract is obtained by a preparation method comprising the following steps: sieving the cloth residues She Cufen, mixing with water according to the material-liquid ratio of 1.
6. An emulsion, characterized in that the emulsion consists of the following components in parts by weight:
phase A: cetearyl alcohol/coco glucoside 1.5% -5.0%, pentaerythritol distearate 1.0% -5.0%, stearic acid 1.0% -3.0%, cetearyl alcohol 0.1% -2.0%;
phase B: 0.01-0.5% of xanthan gum, 1.0-10.0% of butanediol, 2.0-8.0% of glycerol, 0.03-0.1% of EDTA disodium and water TO100;
and C phase: sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80.1% -0.5%;
phase D: 2.0 to 10.0 percent of microcos paniculata extract;
e phase: 0.5 to 1.0 percent of phenoxyethanol/caprylyl glycol/decanediol;
the microcos paniculata leaf extract is prepared by a preparation method comprising the following steps: sieving the cloth residues She Cufen, mixing with water according to the material-liquid ratio of 1.
7. The emulsion of claim 6, wherein the bushy leaf extract in the emulsion has a flavone content of 1.04-4.07mg/mL.
8. The essence is characterized by comprising the following components in parts by weight:
phase A: 0.2 TO 0.5 percent of carbomer, 0.03 TO 0.1 percent of EDTA disodium, 1.0 TO 10.0 percent of butanediol, 0.01 TO 1.0 percent of allantoin and water TO100;
phase B: 0.5 to 1.0 percent of sodium hydroxide;
and C phase: 0.5 to 1.0 percent of microcos paniculata extract;
phase D: 0.5 to 1.0 percent of phenoxyethanol/caprylyl glycol/decanediol;
the microcos paniculata leaf extract is obtained by a preparation method comprising the following steps: sieving the cloth residues She Cufen, mixing with water according to the material-liquid ratio of 1.
9. The essence of claim 8, wherein the bushy leaf extract of the seminal vesicle is 1.04-4.07mg/mL of flavone.
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Address before: 102488 80701, 7th floor, education practice building, 11 Xueyuan North Street, Gongchen sub district office, Fangshan District, Beijing

Patentee before: TAIHE KANGMEI (BEIJING) TRADITIONAL CHINESE MEDICINE INSTITUTE Co.,Ltd.

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