CN112342147A - Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof - Google Patents
Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof Download PDFInfo
- Publication number
- CN112342147A CN112342147A CN202011264508.4A CN202011264508A CN112342147A CN 112342147 A CN112342147 A CN 112342147A CN 202011264508 A CN202011264508 A CN 202011264508A CN 112342147 A CN112342147 A CN 112342147A
- Authority
- CN
- China
- Prior art keywords
- lut
- guilliermondii
- pichia guilliermondii
- pichia
- alternaria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000235048 Meyerozyma guilliermondii Species 0.000 title claims abstract description 104
- 244000052616 bacterial pathogen Species 0.000 title claims abstract description 11
- 230000003042 antagnostic effect Effects 0.000 title claims description 8
- 241000223602 Alternaria alternata Species 0.000 claims abstract description 20
- 241000266349 Alternaria tenuissima Species 0.000 claims abstract description 19
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 16
- 201000010099 disease Diseases 0.000 claims abstract description 15
- 235000012055 fruits and vegetables Nutrition 0.000 claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims description 25
- 238000002360 preparation method Methods 0.000 claims description 23
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 230000002401 inhibitory effect Effects 0.000 claims description 15
- 241000196324 Embryophyta Species 0.000 claims description 11
- 239000012681 biocontrol agent Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000000047 product Substances 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 241000221955 Chaetomium Species 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 5
- 244000061456 Solanum tuberosum Species 0.000 claims description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 241001480037 Microsporum Species 0.000 claims description 3
- 244000053095 fungal pathogen Species 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 244000052769 pathogen Species 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- -1 /or Species 0.000 claims 1
- 239000008121 dextrose Substances 0.000 claims 1
- 241000222195 Ascochyta Species 0.000 abstract description 17
- 241000228453 Pyrenophora Species 0.000 abstract description 11
- 238000004321 preservation Methods 0.000 abstract description 5
- 230000012010 growth Effects 0.000 description 21
- 239000001965 potato dextrose agar Substances 0.000 description 21
- 239000002609 medium Substances 0.000 description 20
- 229920001817 Agar Polymers 0.000 description 16
- 239000008272 agar Substances 0.000 description 16
- 241001447252 Leptospora Species 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000007787 solid Substances 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 241000223600 Alternaria Species 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 5
- 108010080698 Peptones Proteins 0.000 description 5
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 235000019319 peptone Nutrition 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 206010027146 Melanoderma Diseases 0.000 description 4
- 241000732549 Sphaerius Species 0.000 description 4
- 244000000010 microbial pathogen Species 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 235000017784 Mespilus germanica Nutrition 0.000 description 3
- 244000182216 Mimusops elengi Species 0.000 description 3
- 235000000560 Mimusops elengi Nutrition 0.000 description 3
- 235000007837 Vangueria infausta Nutrition 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 244000000005 bacterial plant pathogen Species 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 235000021022 fresh fruits Nutrition 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 235000016623 Fragaria vesca Nutrition 0.000 description 2
- 240000009088 Fragaria x ananassa Species 0.000 description 2
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 2
- 241000605372 Fritillaria Species 0.000 description 2
- 244000241838 Lycium barbarum Species 0.000 description 2
- 235000015459 Lycium barbarum Nutrition 0.000 description 2
- 244000241872 Lycium chinense Species 0.000 description 2
- 235000015468 Lycium chinense Nutrition 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 2
- 241000220324 Pyrus Species 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000015784 hyperosmotic salinity response Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000190150 Bipolaris sorokiniana Species 0.000 description 1
- 241001126268 Cooperia Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000371644 Curvularia ravenelii Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 241000238710 Dermatophagoides Species 0.000 description 1
- 240000001008 Dimocarpus longan Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 244000061508 Eriobotrya japonica Species 0.000 description 1
- 235000009008 Eriobotrya japonica Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000000235 Euphoria longan Nutrition 0.000 description 1
- 241000935235 Fritillaria meleagris Species 0.000 description 1
- 241001547125 Fritillaria thunbergii Species 0.000 description 1
- 241000735234 Ligustrum Species 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 241001106041 Lycium Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 241001533598 Septoria Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241000223230 Trichosporon Species 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 241001247821 Ziziphus Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000019990 fruit wine Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a Pichia guilliermondii strain Meyerozyma guilliermondii lut-Y1 with the preservation number of CGMCC NO.20461, which can antagonize various pathogenic bacteria. The strain can antagonize Alternaria alternata (Alternaria alternata), Alternaria tenuissima (Alternaria tenuissima), Ascochyta leprospora and Deschltzilla (Drechslera spp.), and has wide application value in the aspects of preventing and treating mildew and rot of fruits and vegetables and plant diseases caused by the mildew.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a Pichia guilliermondii strain (Meyerozyma guilliermondii) lut-Y1 capable of antagonizing various pathogenic bacteria, and application thereof in inhibiting growth of plant pathogenic bacteria.
Background
Fruit and vegetable spoilage and plant diseases are two important factors causing yield reduction and threatening food safety, and both are caused by parasitism of pathogenic microorganisms such as mold, bacteria and the like. Pathogenic microorganisms parasitize on fruits and vegetables, which not only change the appearance, smell and color of the fruits and vegetables to cause putrefaction, but also secrete toxins to poison people eating the fruits and vegetables by mistake. The plant infection pathogenic microorganism can cause various plant diseases, such as plant dwarfing, generation of lesion leaves, yield reduction and the like. Therefore, the development of safe and highly effective biocontrol agents for inhibiting spoilage and plant disease using microbial antagonism is one of the most effective ways to solve the problem.
Pichia guilliermondii is a widely available yeast that can be isolated in the course of fermentation of coastal, deep sea, marine algae surfaces, high salt soy sauce and acidic juice beverages. The pichia mondii strains in different seasons have different characteristics, and some strains are used for brewing fruit wine to play a role in increasing the flavor; some can inhibit plant pathogenic bacteria or putrefying bacteria, can be made into biocontrol preparation for keeping loquat, strawberry, pear and other fruits fresh and inhibiting strawberry diseases, and some strains are used for producing xylitol by fermentation.
Alternaria alternata (Alternaria alternata) is one of Alternaria of the order of Trichosporon of the class of mitosporitic spore fungi, is also a main crop pathogenic bacterium, can cause tobacco brown spot, is generated in Shandong, Henan, Anhui, Jilin, Yunnan, Guizhou, Zhejiang and the like in China, and brings serious economic loss to tobacco production. Alternaria alternata is also a fungus causing decay of picked fruits such as pears, hami melons, jujubes and the like. The infection of fruit can cause the epidermis to appear brown and concave round spots, the flesh under the spots is in a black sponge shape, and the commodity is reduced. At present, chemical control is mainly used for preventing and treating alternaria fungal diseases, and the problems of drug resistance, environmental safety and the like caused by the chemical control are increasingly serious.
Alternaria tenuissima (Alternaria tenuissima) is also a main plant pathogenic bacterium of Alternaria, and can cause leaf spot of water wax (Ligustrum obtusifolia Sieb.) and black spot of Fritillaria thunbergii. Black spot is one of important diseases of fritillary, mainly harms leaves, the thunberg region usually begins to get ill in the late 3 th of the month, yellow brown water stain-like disease spots appear at the top of the leaves in the early stage of onset, the diseased part and the healthy part have obvious limits, the disease spots spread to the base part until the overground part of the plant dies early, the underground bulb is thin and small, the serious yield reduction is caused, and especially the disease is more serious in rainy years; meanwhile, the black spot easily overwinter on the damaged plants and the diseased leaves with hypha and conidia, and the damage is infected again in the next year. Therefore, the prevention and control of fritillaria black spot disease is the key point for guaranteeing the development of fritillaria industry.
Ascochyta leptospora (Ascochyta leptospora) is a fungus of Deuteromycotina, Ascochyta and Ascochyta, which can cause leaf spot of peanut and longan. The ascochyta microsporum can cause ryegrass wilt, and brings huge loss to production management of the lawn.
The genus Dermatopteria (Drechslera spp.) is a pathogenic microorganism that causes leaf spotting, leaf blight, root rot and stem rot of a variety of turf grasses. Under the appropriate environmental conditions, the disease condition is rapidly developed, and the lawn can be premature senility and bald spots, which seriously harm the lawn landscape.
Disclosure of Invention
The invention aims to provide a Pichia guilliermondii strain (Meyerozyma guilliermondii) lut-Y1 capable of inhibiting the growth of various pathogenic bacteria, which is preserved in China general microbiological culture Collection center (GCMCC) at 7-28 months in 2020 at the preservation address of: the invention also provides application of the compound in inhibiting various phytopathogens, wherein the number of the compound is CGMCC No. 1, and the number of the compound is CGMCC No. 3.
The invention provides a Pichia guilliermondii strain Meyerozyma guilliermondii lut-Y1 with the preservation number of CGMCCNO.20461, which can antagonize various pathogenic bacteria.
The invention also provides a preparation method of the pichia guilliermondii lut-Y1 fermentation product, which comprises the steps of inoculating the pichia guilliermondii lut-Y1 into a potato glucose liquid culture medium for culture to obtain a fermentation liquid, centrifuging the fermentation liquid, and taking a supernatant to obtain the pichia guilliermondii lut-Y1 fermentation product.
Preferably, the culture conditions are: at 25-30 ℃, 120--1Shaking and culturing for 18-35 h. When the resulting fermentation broth is centrifuged, the parameters conventional in the art may be selected so long as the majority of the fermentation product is present in the centrifuged supernatant, e.g., 4000 r.min-1And centrifuging for 10 min.
The invention also provides a biological control preparation, and the active ingredient of the biological control preparation is the fermentation product of the pichia guilliermondii lut-Y1 prepared by the method.
The invention also provides application of the thallus, the sprout or the biological control preparation of the pichia guilliermondii lut-Y1 strain in preparing products for inhibiting the mildew rot pathogen.
Preferably, the product comprises a fruit and vegetable fresh-keeping preservative, a plant disease control preparation and a medicament.
Preferably, the mould pathogenic fungus is alternaria, alternaria tenuis, ascochyta and/or a mould of the genus drechslera.
The Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 is separated from fruit mold rotten spots of Lycium barbarum and is determined to be the Pichia guilliermondii (Meyerozyma guilliermondii) by ITS region sequence sequencing analysis and morphological identification. The microbiological properties of the yeast strain are: on a yeast extract peptone glucose agar medium (YPDA), a bacterial colony is pink, round, neat in edge, large and thick, and bulged in the middle, is opaque, smooth and moist, and is easy to pick up; white, round, smooth and moist colonies were formed on potato dextrose agar medium (PDA) and wort agar Medium (MEA). The strain can tolerate NaCl with the mass percent of less than 3%, and when the concentration of NaCl is more than 3%, the growth of the yeast is obviously inhibited. The characteristics of the growth curve of the strain are as follows: 0-6h is latent period, 6-18h is exponential growth period, 18-35h is stable growth period, and the decline begins after 36 h.
The Pichia guilliermondii strain (Meyerozyma guilliermondii) lut-Y1 of the present invention is capable of antagonizing Alternaria (Alternaria alternata), Alternaria tenuissima (Alternaria tenuissima), Chaetomium microsporum (Ascocchyta leptospora) and a mold of the genus Derma (Drechslera spp.); the fermentation liquor can be used as a biological control preparation to obviously inhibit the growth of the 4 moulds, and has wide application value in the aspects of preventing and treating mildew and rot of fruits and vegetables and plant diseases caused by the moulds.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 shows the colony morphology and cell morphology of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1.
FIG. 2 shows the comparison of rDNA-ITS sequence of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 with Gen Bank nucleic acid database.
FIG. 3 shows the colony morphology of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 on different media.
FIG. 4 shows the salt tolerance of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1.
FIG. 5 is a graph showing the growth curves of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 in YPDA medium.
FIG. 6 is a graph showing the antagonistic effect of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 against 4 pathogenic bacteria.
FIG. 7 is a graph showing the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent on Alternaria alternata (Alternaria alternata).
FIG. 8 is a graph showing the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent against a mold of the genus Dermatopteria (Drechslera spp.).
FIG. 9 shows the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent against Chaetomium microsporus (Ascochyta leptospora) mold.
FIG. 10 is a graph showing the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent on Alternaria tenuissima (Alternaria tenuissima).
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from conventional biochemicals, unless otherwise specified.
The preparation method of the culture medium comprises the following steps:
potato dextrose agar medium (PDA): weighing 200g, and cutting into 1cm3Boiling potato blocks with different sizes in boiling water for 20min, filtering with 4 layers of gauze, adding 20g of glucose and 15-20g of agar into the filtrate, adding water to 1L, adjusting pH to natural, and sterilizing to obtain PDA solid culture medium; the PDA liquid culture medium is prepared by the same method as the PDA solid culture medium except that agar powder is not added.
Yeast extract peptone glucose agar medium (YPDA): 20g of peptone, 20g of glucose, 10g of yeast extract powder and 15-20g of agar powder, adding water to 1L, naturally adjusting pH, and sterilizing. The YPDA liquid culture medium is prepared by the same method as YPDA solid culture medium except that no agar powder is added.
The formula of the wort agar culture Medium (MEA) is as follows: 130g of wort culture medium and 15-20g of agar powder, adding water to 1L, naturally adjusting the pH value, and sterilizing.
The strain source is as follows: alternaria alternata (Alternaria alternata) is separated from fresh fruit mildew rot spots of medlar produced by medlar production cooperative society of Yongden county, Lanzhou, Gansu province and stored in the laboratory, and the specific separation and identification process is shown in the literature (Yuanjun, Li Hujun, Jiahong Sha, and the like. separation and identification of mildew rot pathogenic fungi in the airing process of the fresh fruits of Yongden medlar. food industry science and technology 2016,37(21): 135. 138.).
Dermatopteria (Drechslera spp.) mold, Ascochyta leptospora (Ascochyta leptospora) and Alternaria tenuisiana (Alternaria tenuissima) were given by the plant pathology laboratory of the Soft. agri, Lanzhou university, and the specific isolation and identification process was described in the literature (Mashi, perennial ryegrass-endophytic fungi symbiont disease resistance and its research on the disease resistance mechanism of Helminthosporium rothii (Bipolaris sorokiniana) [ D ].2015 ]
Example 1
The Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 of the invention is isolated from the fresh fruit mold rot spot of Lycium barbarum produced by Gongdeng county Lycium production Cooperia, Lanzhou, Gansu. The separation process is as follows: the fermented fruit of Chinese wolfberry is collected from the co-production bed of Chinese wolfberry of Chuanzhen county of Yongdeng county of Lanzhou, Gansu province in 2014 8 months, is transported to a laboratory of the university for separation and purification on the same day, adopts a tissue separation method, selects and dries 3d diseased fruits typical of disease spots to be washed clean, cuts off a small tissue with the size of 5mm multiplied by 5mm at the boundary of disease keys, uses 2% sodium hypochlorite for surface disinfection for 8min, uses sterile water for 5 times, uses 75% alcohol for disinfection for 30s, uses sterile water for 8 times, moves to a sterilization culture dish, adds 1mL of sterile water for mashing, uses an inoculating loop to dip a tissue fluid containing bacteria on a PDA flat plate for scribing, cultures at 28 ℃ and picks up a single colony, and a pure culture after continuous purification for 3 times is stored on a slant PDA culture medium and is stored in a refrigerator at 4 ℃ for later use; representative strains were placed on different media, respectively. The yeast is cultured in malt extract agar medium at 28 deg.C for 3d, and the colony culture characteristics are observed and recorded. Selecting yeast, making into water-filled tablet, and observing shape under common optical microscope; the yeast is picked up, stuck on a sample table, placed in liquid nitrogen for quick freezing, and then observed under a scanning electron microscope for morphological characteristics such as cells and the like (figure 1). The test strains were inoculated to PDA medium and cultured at 25 ℃ for 3-5 days, and then sent to Dalibao bioengineering GmbH for strain genomic DNA extraction, PCR amplification and ITS region sequence sequencing (FIG. 2), and the obtained sequences were compared with GenBank nucleic acid database (www.ncbi.nlm.nih.gov/BLAST) (FIG. 3). Finally, the strain is determined to be Pichia guilliermondii (Meyerozyma guilliermondii) according to the sequence sequencing analysis and morphological identification of the ITS region. The strain is preserved in China general microbiological culture Collection center (GCMCC) at 7 months and 28 days in 2020, with the preservation addresses as follows: no. 3 of Xilu No. 1 of Beijing, Chaoyang, and the preservation number is CGMCC NO. 20461.
Example 2
The specific experimental process of the antibacterial performance of the Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 strain CGMCC NO.20461 comprises the following steps:
(1) activating strains: subpackaging the sterilized PDA solid culture medium in 50mL test tubes, pouring 15mL of the PDA solid culture medium in each test tube, obliquely placing the test tubes on a table top to prepare a slant culture medium, and cooling and solidifying the culture medium; pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 strain CGMCC NO.20461 was streaked on a slant medium with an inoculating needle and cultured at room temperature for 2-3 days. The preparation of the bacterial suspension comprises the following steps: 4mL of sterile water is added into the slant strains respectively, and the mixture is fully oscillated to prepare bacterial suspension.
(2) And (4) observing colony morphology: pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 were streaked onto 3 solid medium plates and observed for colony morphology after 2 days of inverted culture at 30 ℃. The 3 solid culture media are respectively: yeast extract peptone glucose agar medium (YPDA), malt extract agar Medium (MEA) and potato glucose agar medium (PDA).
(3) Determination of salt resistance: mu.l of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 bacterial suspension was inoculated into 50ml of YPDA-NaCl liquid medium containing 0%, 0.5%, 1%, 3%, 5% by mass, and subjected to shaking culture at 30 ℃ and 120r/min for 24 hours, and then the absorbance of the culture was measured at a wavelength of 660nm using a spectrophotometer. Each treatment was set to 5 replicates.
(4) And (3) measuring a growth curve: 50 μ l of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 bacterial suspension was added to 50ml of YPDA liquid medium, shaking-cultured at 30 ℃ for 24 hours at 160r/min in a shaker, and the absorbance of the culture solution was measured every 2 hours at a wavelength of 660nm by a spectrophotometer, and the growth curve was plotted with absorbance as the vertical axis and time as the horizontal axis. Each treatment was set to 5 replicates.
(5) Antagonism: adopting a flat plate opposing method. Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 and the test strains (Alternaria alternata, a mold of the genus Deschleria (Drechslera spp.), Chaetomium microsporus (Ascochyta leptospora) and Alternaria tenuissima) were inoculated on the same PDA plate, respectively, and the colony growth was observed.
(6) Preparation of the biological control preparation: inoculating 500 μ L of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 strain suspension into PDA liquid culture medium, and culturing at 30 deg.C for 160 r.min-1After shaking culture for 18h, the fermentation liquor is cultured at 4000 r.min-1Centrifuging for 10min to obtain supernatant as biological control preparation with OD value of 1.6-2.0.
(7) Preparing a fungus cake for inoculation: alternaria alternata (Alternaria alternate), Alternaria tenuissima (Alternaria tenuissima), Ascochyta leptospora, a mold of the genus Deschschltzilla (Drechslera spp.) were prepared as test bacteria. Respectively adding 4mL of sterile water into slant strains of the bacteria to be tested, fully oscillating, preparing bacterial suspension, coating 1mL of the bacterial suspension in a PDA solid culture medium flat plate, culturing at 24-28 ℃ for 5-7 days until the strains grow to fill the flat plate, and taking a bacteria cake to be tested with the diameter of 1cm from the center of the flat plate by using a sterile puncher.
(8) The biological control preparation has the following inhibition effect on bacteria to be tested: a biological control preparation with gradient concentration, namely PDA solid culture medium, is prepared according to the proportion of table 1, sterilized and then subpackaged in culture dishes with the diameter of 9cm, and each dish is 25 mL. Sticking the bacterial cake to be tested to the center of a biological control preparation-PDA solid culture medium flat plate, repeating each strain for 3 times, placing culture dishes inoculated with the bacterial and the fungal cake to a constant-temperature incubator at 30 ℃ for 5-7d respectively, observing the colony morphology, measuring the diameter of a growth ring of the bacteria to be tested, and performing single-factor variance analysis on the diameter change of the growth ring of the bacteria to be tested by using SPSS statics 21 statistical software.
TABLE 1 formulation ratio of PDA culture medium as biological control agent with gradient concentration
The experimental results are as follows:
(1) the Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 of the present invention was cultured on wort agar medium for 3d to form colonies with a diameter of 0.5-1 cm. The colony is pink, round, irregular in edge wrinkles, convex in surface, smooth and opaque (fig. 1). The cell shape is oval, and has the shape and size characteristics of yeast. The rDNA-ITS region primer is used for PCR amplification to obtain the rDNA-ITS sequence of 552bp of the strain, and BLAST comparison is carried out on the rDNA-ITS sequence and the Pichia guilliermondii (Meyerozyma guilliermondii) which is recorded in GenBank, and the sequence similarity reaches 100 percent (figure 2). Based on the above morphological and molecular biological characteristics, the strain was identified as Pichia guilliermondii (Meyerozyma guilliermondii).
The rDNA-ITS sequence of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 is as follows:
FIG. 1 shows the colony morphology and cell morphology of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1.
FIG. 2 shows the comparison of rDNA-ITS sequence of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 with Gen Bank nucleic acid database.
(2) The colony of the Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 on a yeast extract peptone glucose agar medium (YPDA) is pink, round, neat in edge, large and thick, and has a bulged middle part, is opaque, smooth and moist, and is easy to pick up; white, round, smooth and moist colonies were formed on potato dextrose agar medium (PDA) and on wort agar Medium (MEA) (fig. 3).
FIG. 3 shows the colony morphology of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 on different media.
(2) The Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 strain of the present invention was able to tolerate NaCl at a mass percentage of 3% or less, and significantly inhibited the growth of the yeast when the concentration of NaCl was greater than 3% by mass (FIG. 4).
FIG. 4 shows the salt tolerance of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1.
(3) The growth curve of the Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 strain is characterized in that: 0-6h is incubation period, 6-18h is exponential growth period, 18-35h is stationary growth period, and beginning to decline at 36h (fig. 5).
FIG. 5 is a graph showing the growth curves of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 in YPDA medium.
(4) The Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 of the present invention antagonized Alternaria alternata (Alternaria alternata), a mold of the genus Detrichella (Drechslera spp.), Septoria microsporus (Ascochyta leptospora) and Alternaria tenuissima (Alternaria tenuissima) (FIG. 6).
FIG. 6 is a graph showing the antagonistic effect of Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 against 4 pathogenic bacteria.
(5) The biological control preparation of the invention has obvious inhibition effect on the growth of alternaria alternate. Compared with the control, the culture medium is added with biological control preparations with the volume percentages of 20 percent, 30 percent and 40 percent, so that the diameters of Alternaria spores are respectively reduced by 26 percent, 75 percent and 91 percent (figure 7).
FIG. 7 is a graph showing the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent on Alternaria alternata (Alternaria alternata).
(6) The biological control preparation of the invention has obvious inhibition effect on the growth of Deerhella (Drechslera spp.). Compared with the control, the culture medium is added with 30 percent and 40 percent of biological control agent by volume, so that the diameters of the mould colonies of the Dermatophagoides respectively are obviously reduced by 52 percent and 68 percent (figure 8).
FIG. 8 is a graph showing the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent against a mold of the genus Dermatopteria (Drechslera spp.).
(7) The biological control preparation of the invention has obvious inhibition effect on the growth of Ascochyta leptospora. Addition of 10%, 20%, 30%, 40% by volume of biocontrol agent to the culture medium resulted in a significant reduction in colony diameter of Ascochyta leptospora (Ascochyta leptospora) of 51%, 90%, 97%, 99%, respectively, compared to the control (fig. 9).
FIG. 9 shows the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent against Chaetomium microsporus (Ascochyta leptospora) mold.
(8) The biological control preparation of the invention has obvious inhibition effect on the growth of Alternaria tenuissima. Compared with a control, the culture medium is added with biological control preparations with the volume percentages of 10%, 20%, 30% and 40%, so that the diameters of Alternaria tenuissima colonies can be respectively and remarkably reduced by 90%, 96% and 100% (figure 10).
FIG. 10 is a graph showing the inhibitory effect of a Pichia guilliermondii (Meyerozyma guilliermondii) lut-Y1 biocontrol agent on Alternaria tenuissima (Alternaria tenuissima).
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> university of Rituo-Risk of Lanzhou
<120> Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 552
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
tcctacctga tttgaggtca aacttgtttg gttgttgtaa ggccgggcca acaataccag 60
aaatatcccg ccacaccatt caacgagttg gataaaccta atacattgag aggtcgacag 120
cactatctag tactacccat gccaatactt ttcaagcaaa cgcctagtcc gactaagagt 180
atcactcaat accaaacccg ggggtttgag agagaaatga cgctcaaaca ggcatgccct 240
ctggaatacc agagggcgca atgtgcgttc aaagattcga tgattcacga aaatctgcaa 300
ttcatattac ttatcgcatt tcgctgcgtt cttcatcgat gcgagaacca agagatccgt 360
tgttgaaagt tttgaagatt aattcaaaat ttgactaact gtaaaaataa ttaaattgtg 420
ttttgttaaa cctctggccc aacctatctc taggccaaac caaagcaaga gttctgtatc 480
aaaaagacac tgtgtgtaag gtttttcgcc gcgcagttaa gcgctggcaa aagaatactg 540
taatgatcct tc 552
Claims (7)
1. A Pichia guilliermondii strain Meyerozyma guilliermondii lut-Y1 with CGMCC NO.20461 is capable of antagonizing multiple pathogenic bacteria.
2. The method for preparing fermentation product of Pichia guilliermondii lut-Y1 of claim 1, wherein: the pichia guilliermondii lut-Y1 of claim 1 is inoculated in a potato dextrose liquid culture medium for culture to obtain a fermentation liquid, and the fermentation liquid is centrifuged to obtain a supernatant to obtain a pichia guilliermondii lut-Y1 fermentation product.
3. The method of claim 2, wherein: the culture conditions are as follows: at 25-30 ℃, 120--1Shaking and culturing for 18-35 h.
4. A biological control formulation characterized by: the active ingredient of the biological control preparation is a fermentation product of Pichia guilliermondii lut-Y1 prepared by the method of claim 2 or 3.
5. The use of a thallus, a sprout, or a biocontrol agent of claim 1 from the strain pichia guilliermondii lut-Y1 for the preparation of a product for inhibiting pathogenic fungi of mold rot.
6. Use according to claim 5, characterized in that: the product comprises a fruit and vegetable fresh-keeping preservative, a plant disease control preparation and a medicament.
7. Use according to claim 5 or 6, characterized in that: the mildew rot pathogen is Alternaria alternata, Alternaria tenuissima, Chaetomium microsporum and/or a mold of Deerhermella.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011264508.4A CN112342147A (en) | 2020-11-12 | 2020-11-12 | Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011264508.4A CN112342147A (en) | 2020-11-12 | 2020-11-12 | Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112342147A true CN112342147A (en) | 2021-02-09 |
Family
ID=74363426
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011264508.4A Pending CN112342147A (en) | 2020-11-12 | 2020-11-12 | Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112342147A (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20140067947A (en) * | 2012-11-27 | 2014-06-05 | 한국생명공학연구원 | A novel thermostable yeast pichia guilliermondii y-2 and use thereof |
CN107460134A (en) * | 2017-06-29 | 2017-12-12 | 江苏大学 | The Pichia guilliermondii of one plant of control the operatic circle postharvest disease |
CN107937284A (en) * | 2017-11-23 | 2018-04-20 | 青岛农业大学 | A kind of Pichia guilliermondii and its application |
WO2018206419A1 (en) * | 2017-05-12 | 2018-11-15 | Syngenta Participations Ag | Microbiocidal heterobicyclic derivatives |
CN113215016A (en) * | 2021-01-22 | 2021-08-06 | 兰州理工大学 | Bacillus amyloliquefaciens and application thereof |
-
2020
- 2020-11-12 CN CN202011264508.4A patent/CN112342147A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20140067947A (en) * | 2012-11-27 | 2014-06-05 | 한국생명공학연구원 | A novel thermostable yeast pichia guilliermondii y-2 and use thereof |
WO2018206419A1 (en) * | 2017-05-12 | 2018-11-15 | Syngenta Participations Ag | Microbiocidal heterobicyclic derivatives |
CN107460134A (en) * | 2017-06-29 | 2017-12-12 | 江苏大学 | The Pichia guilliermondii of one plant of control the operatic circle postharvest disease |
CN107937284A (en) * | 2017-11-23 | 2018-04-20 | 青岛农业大学 | A kind of Pichia guilliermondii and its application |
CN113215016A (en) * | 2021-01-22 | 2021-08-06 | 兰州理工大学 | Bacillus amyloliquefaciens and application thereof |
Non-Patent Citations (3)
Title |
---|
VORONIN LV等: "checklist of fungi and fungi-like organisms on the common reed Phragmites australis", ASIAN JOURNAL OF MYCOLOGY, vol. 4, no. 2, 2 December 2021 (2021-12-02), pages 67 - 113 * |
周海莲等: "季也蒙毕赤酵母对草莓采后灰葡萄孢霉的抑制机理", 食品科学, 8 July 2014 (2014-07-08), pages 6 - 11 * |
袁惠君;李虎军;贾鸿震;巩慧玲;冯再平;: "永登枸杞鲜果晾晒过程中霉腐病原真菌的分离鉴定", 食品工业科技, no. 21, 1 November 2016 (2016-11-01), pages 135 - 138 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230174925A1 (en) | Bacillus velezensis, culture method therefor and application thereof | |
CN102533617B (en) | Bacillus subtilis strain and application thereof | |
CN104531578A (en) | Lactobacillus plantarum strain having functions of effectively degrading nitrite and strongly producing acid and application of lactobacillus plantarum strain | |
CN112175888B (en) | Bacillus belgii Hsg1949 and application thereof | |
Zhang et al. | Biological control of postharvest blue mold of oranges by Cryptococcus laurentii (Kufferath) Skinner | |
CN113215016B (en) | Bacillus amyloliquefaciens and application thereof | |
CN111235039B (en) | Culture medium for culturing metarhizium anisopliae with high toxicity, preparation and application thereof | |
CN115806913B (en) | Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and application thereof | |
CN109234210A (en) | For the bacillus subtilis of antagonism grey mould fruit rot of strawberry and its preparation of biocontrol agent and application | |
CN114032179B (en) | Industrial cannabis endophytic fungus for producing CBD and application thereof | |
CN108396002B (en) | Bacillus licheniformis and application thereof in preventing and treating sweet melon fusarium wilt | |
CN110591932A (en) | Yeast MA for controlling postharvest diseases of fruits and vegetables and use method thereof | |
CN110527639A (en) | A kind of U.S. pole plum surprise yeast and its application | |
CN112342147A (en) | Pichia guilliermondii strain capable of antagonizing various pathogenic bacteria and application thereof | |
CN112931044B (en) | Culture medium and culture method of harrow tooth bacteria | |
CN112481141B (en) | Debaryomyces hansenii strain and application thereof in preparation of fruit preservative | |
CN110628655B (en) | Yeast Lg-3 for controlling postharvest diseases of fruits and vegetables and using method thereof | |
CN109913379B (en) | High-esterase-activity Meiji yeast and application thereof in fermentation of mead | |
CN107523525B (en) | Bacillus belgii and application thereof in prevention and treatment of walnut canker | |
CN111778165A (en) | Aspergillus niger DFY1 and application thereof | |
CN117305135B (en) | Trichoderma pseudokoningii T0027 and application thereof in preventing and treating soft rot of kiwi fruits | |
CN112195107B (en) | Erwinia glauca strain and application thereof | |
CN116240136B (en) | Leuconostoc mesenteroides WZ-44 for antagonizing erwinia amylovora and Asian erwinia amylovora and application thereof | |
CN116064241B (en) | Shell mould YAFEF037 strain and separation method and application thereof | |
CN116333891B (en) | Biocontrol bacterium JSNL-TX55 for gray mold and anthracnose of strawberries and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |