CN112322477A - Fermentation system and fermentation method thereof - Google Patents

Abstract

The invention provides a fermentation system and a fermentation method thereof, wherein the fermentation system comprises: the fermentation tank is connected with the control system to control the working state of the fermentation tank, the top of the fermentation tank is provided with a bacterial liquid inlet and a feed liquid inlet for the entry of the bacterial liquid and the feed liquid, and the bottom of the fermentation tank is provided with a liquid outlet for the discharge of fermentation products; the fermentation tank is internally provided with a liquid ejector and a micro-interface generator, the micro-interface generator is arranged at the bottom in the fermentation tank, the liquid ejector is arranged at the upper part in the fermentation tank, the side wall of the fermentation tank is provided with a cleaning water inlet and an air inlet, the air inlet is introduced into the micro-interface generator through a pipeline, and the liquid ejector is connected with the cleaning water inlet through a pipeline. The fermentation system solves the problem of low fermentation efficiency caused by the fact that air and fermentation liquor cannot be fully mixed in the fermentation tank in the prior art, and saves labor cost for cleaning and field operation.

Description

Fermentation system and fermentation method thereof

Technical Field

The invention relates to the field of fermentation, in particular to a fermentation system and a fermentation method thereof.

Background

Industrial fermentation is an industrial process for converting fermentation raw materials into microbial products required by human beings through the vital activities of microorganisms. In our country, the fermentation industry has been developed greatly in recent years as an important branch in biotechnology, and new fermentation industries (e.g., amino acids, enzyme preparations, organic acids, single-cell proteins, starch sugars, etc.) have been increasing at an average rate of 21% per year. To date, China has formed a fermentation industrial system with various varieties, complete categories and a considerable scale, and the product application covers various industries such as medicine, sanitation, light industry, agriculture, energy, environmental protection and the like.

In aerobic fermentation, the dissolved oxygen content is a limiting factor of thallus growth and fermentation, and in the traditional aerobic fermentation process, the dissolved oxygen concentration in the fermentation liquor is generally only 7-8 ppm, so that the fermentation efficiency is low. Therefore, it is an important direction for the development of modern fermentation industry to seek a fermentation apparatus with higher gas-liquid mass transfer efficiency to increase the dissolved oxygen concentration and improve the fermentation yield.

In addition, the existing fermentation process requires a lot of manpower, including access to the inside of the tank through a manhole for washing, and on-site operation of valves of various pipes. Therefore, the labor cost is improved, and the production safety is reduced.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The first purpose of the invention is to provide a fermentation system, which is characterized in that a micro-interface generator and a liquid ejector are arranged in a fermentation tank, on one hand, the liquid ejector can efficiently crush water for cleaning a tank body into micron-sized liquid drops, and the micron-sized liquid drops are collided above the fermentation tank through the ejector to replace manual cleaning, on the other hand, air is efficiently crushed into micron-sized bubbles through the micro-interface generator and dispersed into fermentation liquor to form a micro-interface system, so that the gas-liquid interface area in a reverse gas-liquid is increased by tens of times, the mass transfer rate of oxygen to the fermentation liquor is greatly increased, the concentration of dissolved oxygen and the macroscopic fermentation rate are increased, furthermore, an intelligent cleaning device is adopted to replace manual cleaning, a PLC (or DCS, PLC and DCS) control system is adopted to carry out remote control on production, and the production.

The second purpose of the invention is to provide a method for fermentation by adopting the fermentation system, the fermentation method is simple and convenient to operate, the obtained fermentation product has high purity and good fermentation effect, and the method is worthy of wide popularization and application.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the present invention provides a fermentation system comprising: the fermentation tank is connected with the control system to control the working state of the fermentation tank, a bacterial liquid inlet and a feed liquid inlet are formed in the top of the fermentation tank and used for allowing bacterial liquid and feed liquid to enter, and a liquid outlet is formed in the bottom of the fermentation tank and used for discharging fermentation products;

the fermentation tank is internally provided with a liquid ejector and a micro-interface generator, the micro-interface generator is arranged at the bottom in the fermentation tank, the liquid ejector is arranged at the upper part in the fermentation tank, the side wall of the fermentation tank is provided with a cleaning water inlet and an air inlet, the air inlet is communicated into the micro-interface generator through a pipeline, and the liquid ejector is connected with the cleaning water inlet through a pipeline.

In the aerobic fermentation process of the fermentation system in the prior art, generally, the fermentation yield is not high due to low gas-liquid mass transfer efficiency, and the invention provides a novel fermentation system for solving the technical problems.

Preferably, the top of the liquid ejector is a semicircular arc surface, a plurality of injection ports are sequentially arranged on the semicircular arc surface, the injection direction of the injection ports faces the top of the fermentation tank, and the cleaning water inlet is connected with the bottom of the liquid ejector through a pipeline.

The liquid ejector is mainly connected with a cleaning water inlet through a pipeline, cleaning water is introduced into the liquid ejector and is ejected through the ejection openings on the semicircular arc surface of the liquid ejector, the semicircular arc surface structure is designed to improve the cleaning effect and ensure that the ejected cleaning water can carry out all-around cleaning on the wall surface of a fermentation tank, and in addition, preferably, a net surface with a plurality of micropores uniformly distributed is paved in each ejection opening, so that the ejected water is broken and dispersed into fog to improve the mass transfer effect and also play a role in achieving the corresponding effect of the micro-interface generator.

Preferably, the micro-interface generator is disposed opposite the liquid injector, the reason for the opposite disposition being to enhance the effect of the mutual cooperation between the two.

It should be noted that the micro-interface generator is preferably located relatively low in the fermenter so that air can enter the fermenter to fill the whole fermenter, and if it is located too high, it may affect the dispersion effect between oxygen and bacteria liquid and feed liquid.

Preferably, the micro-interface generator is a single one that can meet practical process requirements, and its specific type is preferably a pneumatic micro-interface generator because the pneumatic type is relatively low cost and easy to install.

The micro-interface generator in the fermentation tank breaks air into micro-bubbles with micron scale, and releases the micro-bubbles into the fermentation liquid, so that the phase boundary mass transfer area between oxygen and the fermentation liquid in the fermentation process is increased, two phases are in full contact, the concentration of dissolved oxygen in the fermentation liquid is improved, the fermentation efficiency is improved, and the fermentation time is shortened.

It will be appreciated by those skilled in the art that the micro-interface generator used in the present invention is described in the prior patents of the present inventor, such as the patents of application numbers CN201610641119.6, CN201610641251.7, CN201710766435.0, CN106187660, CN105903425A, CN109437390A, CN205833127U and CN 207581700U. The detailed structure and operation principle of the micro bubble generator (i.e. micro interface generator) is described in detail in the prior patent CN201610641119.6, which describes that "the micro bubble generator comprises a body and a secondary crushing member, wherein the body is provided with a cavity, the body is provided with an inlet communicated with the cavity, the opposite first end and second end of the cavity are both open, and the cross-sectional area of the cavity decreases from the middle of the cavity to the first end and second end of the cavity; the secondary crushing member is disposed at least one of the first end and the second end of the cavity, a portion of the secondary crushing member is disposed within the cavity, and an annular passage is formed between the secondary crushing member and the through holes open at both ends of the cavity. The micron bubble generator also comprises an air inlet pipe and a liquid inlet pipe. "the specific working principle of the structure disclosed in the application document is as follows: liquid enters the micro-bubble generator tangentially through the liquid inlet pipe, and gas is rotated at a super high speed and cut to break gas bubbles into micro-bubbles at a micron level, so that the mass transfer area between a liquid phase and a gas phase is increased, and the micro-bubble generator in the patent belongs to a pneumatic micro-interface generator.

In addition, the first patent 201610641251.7 describes that the primary bubble breaker has a circulation liquid inlet, a circulation gas inlet and a gas-liquid mixture outlet, and the secondary bubble breaker communicates the feed inlet with the gas-liquid mixture outlet, which indicates that the bubble breakers all need to be mixed with gas and liquid, and in addition, as can be seen from the following drawings, the primary bubble breaker mainly uses the circulation liquid as power, so that the primary bubble breaker belongs to a hydraulic micro-interface generator, and the secondary bubble breaker simultaneously introduces the gas-liquid mixture into an elliptical rotating ball for rotation, thereby realizing bubble breaking in the rotating process, so that the secondary bubble breaker actually belongs to a gas-liquid linkage micro-interface generator. In fact, the micro-interface generator is a specific form of the micro-interface generator, whether it is a hydraulic micro-interface generator or a gas-liquid linkage micro-interface generator, however, the micro-interface generator adopted in the present invention is not limited to the above forms, and the specific structure of the bubble breaker described in the prior patent is only one of the forms that the micro-interface generator of the present invention can adopt.

Furthermore, the prior patent 201710766435.0 states that the principle of the bubble breaker is that high-speed jet flows are used to achieve mutual collision of gases, and also states that the bubble breaker can be used in a micro-interface strengthening reactor to verify the correlation between the bubble breaker and the micro-interface generator; moreover, in the prior patent CN106187660, there is a related description on the specific structure of the bubble breaker, see paragraphs [0031] to [0041] in the specification, and the accompanying drawings, which illustrate the specific working principle of the bubble breaker S-2 in detail, the top of the bubble breaker is a liquid phase inlet, and the side of the bubble breaker is a gas phase inlet, and the liquid phase coming from the top provides the entrainment power, so as to achieve the effect of breaking into ultra-fine bubbles, and in the accompanying drawings, the bubble breaker is also seen to be of a tapered structure, and the diameter of the upper part is larger than that of the lower part, and also for better providing the entrainment power for the liquid phase.

Since the micro-interface generator was just developed in the early stage of the prior patent application, the micro-interface generator was named as a micro-bubble generator (CN201610641119.6), a bubble breaker (201710766435.0) and the like in the early stage, and is named as a micro-interface generator in the later stage along with the continuous technical improvement, and the micro-interface generator in the present invention is equivalent to the micro-bubble generator, the bubble breaker and the like in the prior art, and has different names.

In summary, the micro-interface generator of the present invention belongs to the prior art, although some micro-interface generators belong to the pneumatic type micro-interface generator, some micro-interface generators belong to the hydraulic type micro-interface generator, and some micro-interface generators belong to the gas-liquid linkage type micro-interface generator, the difference between the types is mainly selected according to the different specific working conditions, and the connection between the micro-interface generator and the reactor and other devices, including the connection structure and the connection position, is determined according to the structure of the micro-interface generator, which is not limited.

Preferably, the two sides of the bottom of the fermentation tank are flat, a bulge is formed upwards in the center of the bottom of the fermentation tank, and the liquid outlets are respectively arranged in the flat positions on the two sides of the bottom of the fermentation tank. The reason why the center position is protruded upward is to discharge the fermentation product generated by aerobic fermentation from the discharge hole efficiently, and a small amount of the fermentation product remains if the bottom is flat, so it is preferable to perform the protrusion of the center position of the bottom of the fermentation tank upward according to the embodiment of the present invention.

Preferably, a stirring paddle is arranged at the central bulge of the bottom in the fermentation tank to play a role in accelerating discharging. The bellied position sets up the stirring rake also is in order to improve fermentation product exhaust efficiency, can also promote fermentation efficiency through the stirring moreover.

Preferably, the fermentation system of the present invention further comprises a plurality of high temperature steam pipelines for sterilizing the fermentation tank, and the pipelines are used for sterilizing the feed liquid, the bacterial liquid and the cleaning water respectively.

Preferably, an ultrasonic sterilization device is arranged in the fermentation tank and is tightly attached to the inner wall of the fermentation tank, so that steam can be introduced into the fermentation tank through a steam pipeline and the ultrasonic sterilization device can be used for assisting sterilization.

Preferably, the number of the washing water inlets arranged on the side wall of the fermentation tank is two, wherein one washing water inlet is connected with the liquid ejector through a pipeline, and the other washing water inlet is arranged at a position close to the bottom of the fermentation tank. Therefore, the effect of cleaning the upper part in the fermentation tank can be achieved, and the effect of cleaning the lower part in the fermentation tank can also be achieved.

In addition, the invention also provides a fermentation method, which comprises the following steps:

crushing the cleaning water into micron-sized micro-droplets to clean the interior of the fermentation tank;

dispersing and crushing the air micro interface, mixing with a bacterial liquid and a feed liquid, carrying out aerobic fermentation, discharging and collecting a fermentation product.

In a word, the fermentation method of the invention is characterized in that the micro-interface generator is arranged in the fermentation tank, so that before the air and the fermentation liquor are fermented, the micro-interface generator breaks the air into micro bubbles with the diameter of more than or equal to 1 mu m and less than 1mm, the air is contacted with the fermentation liquor in a micro bubble state, the phase boundary mass transfer area between the oxygen and the fermentation liquor in the fermentation process is increased, two phases are fully contacted, the dissolved oxygen concentration in the fermentation liquor is improved, the fermentation efficiency is improved, the fermentation time is shortened, and the problem of low fermentation efficiency in the prior art is solved.

Especially in this fermentation system, realized automatic fermentation cylinder cleaning process and intelligent control process through adding control system, further practiced thrift manufacturing cost.

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the fermentation system, the micro-interface generator and the liquid ejector are arranged in the fermentation tank, so that on one hand, the liquid ejector can efficiently crush water for cleaning the tank body into micron-sized liquid drops, and the micron-sized liquid drops are collided above the fermentation tank through the ejector to replace manpower for cleaning, on the other hand, air is efficiently crushed into micron-sized bubbles through the micro-interface generator and is dispersed into fermentation liquor to form a micro-interface system, the gas-liquid phase interface area in reverse gas-liquid is increased by tens of times, the mass transfer rate of oxygen to the fermentation liquor is greatly increased, and the concentration of dissolved oxygen and the macro-fermentation rate are increased;

(2) the fermentation system adopts an intelligent cleaning device to replace manual cleaning, adopts a PLC (or DCS, PLC and DCS) control system to remotely control the production, and accords with the intellectualization of the production;

(3) the fermentation method is simple and convenient to operate, and the obtained fermentation product has high purity and good fermentation effect, and is worthy of wide popularization and application.

Drawings

Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:

FIG. 1 is a schematic structural diagram of a fermentation system provided in an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a mesh surface of a fermentation system provided in an embodiment of the present invention.

Description of the drawings:

10-a fermentation tank; 101-a bacterium liquid inlet;

102-feed liquid inlet; 103-a liquid ejector;

104-a micro-interface generator; 105-an air inlet;

106-wash water inlet; 1031-jet orifice;

1032-mesh side; 107-a liquid outlet;

108-a stirring paddle; 109-ultrasonic sterilization device;

and 20, controlling the system.

Detailed Description

The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and the detailed description, but those skilled in the art will understand that the following described embodiments are some, not all, of the embodiments of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In order to more clearly illustrate the technical solution of the present invention, the following description is made in the form of specific embodiments.

Examples

Referring to fig. 1, a fermentation system according to an embodiment of the present invention mainly includes a fermentation tank 10 and a control system 20, a liquid ejector 103 and a micro-interface generator 104 are disposed in the fermentation tank 10, the liquid ejector 103 and the micro-interface generator 104 are disposed opposite to each other, a side wall of the fermentation tank 10 is provided with a cleaning water inlet 106 and an air inlet 105, a top of the fermentation tank 10 is provided with a bacterial liquid inlet 101 and a feed liquid inlet 102 for entering of bacterial liquid and feed liquid, the micro-interface generator 104 is connected to the air inlet 105 through a pipeline, and the air is crushed into micro-bubbles with a diameter of micron order before a fermentation reaction, so as to increase a phase boundary mass transfer area between oxygen and fermentation liquid in a fermentation process, so that the two phases are in full contact with each other, thereby increasing a dissolved oxygen concentration in the fermentation liquid.

The liquid ejector 103 is connected with the cleaning water inlet 106 through a pipeline, the top surface of the liquid ejector 103 is in a semicircular arc surface, a plurality of injection ports 1031 are sequentially arranged on the semicircular arc surface, the injection directions of the injection ports 1031 face the top of the fermentation tank 10, the cleaning water inlet 106 is connected with the bottom of the liquid ejector 103 through a pipeline, and the cleaning water is crushed into micro-droplets with micron-sized diameters and sprayed out from the injection ports 1031 protruding from the surface so as to collide the top of the tank body for efficient cleaning. In order to improve the cleaning effect, a mesh surface 1032 with a plurality of uniformly distributed micropores is laid in the spray opening 1031, the number of the mesh surfaces 1032 is not limited, in order to spray the cleaning water in a mist form and improve the cleaning mass transfer effect, and the structure of the mesh surface 1032 is specifically shown in fig. 2.

The bottom of the fermentation tank 10 is provided with a liquid outlet 107 for discharging waste water and waste gas and discharging the product. The bottom both sides of fermentation cylinder 10 level, and central point puts and upwards forms the arch the level and smooth position in both sides of fermentation cylinder 10 bottom is provided with respectively the liquid outlet 107, bottom center bulge department is provided with stirring rake 108 in the fermentation cylinder 10 in order to play the effect of arranging the material with higher speed, and the direction of blade is up to be used for the fermentation cylinder 10 bottom to wash, the stirring when fermentation and flowing back, the rotational speed can infinitely variable control.

For the purpose of cleaning, two cleaning water inlets 106 are provided on the side wall of the fermentation tank 10, wherein one of the cleaning water inlets 106 is connected to the liquid sprayer 103 through a pipeline, and the other cleaning water inlet 106 is provided near the bottom of the fermentation tank 10. This allows for efficient cleaning of various locations within the fermentor 10.

The fermentation tank 10 of the present invention can also realize the disinfection function, and each feeding pipeline is correspondingly provided with a steam pipeline, and the inner side wall surface of the fermentation tank 10 is correspondingly provided with an ultrasonic sterilization device 109 to assist the steam pipeline in sterilization.

This embodiment also includes a PLC (or DCS, PLC and DCS) control system 20: is connected with the sensor of the fermentation tank 10 and is used for intelligently controlling the process operation and parameters, thereby realizing the remote control of the production and conforming to the intellectualization of the production.

The specific fermentation process of the embodiment of the invention is as follows:

(1) cleaning: the cleaning water above the fermentation tank 10 is conveyed to the inside of the liquid sprayer 103, sprayed out through the spray opening 1031 on the arc surface, efficiently crushed into micron-sized (d is more than or equal to 1 mu m and less than 1mm) liquid drops through the mesh surface 1032, sprayed out through the spray opening 1031 protruding from the surface of the liquid sprayer 103, collided with the top of the fermentation tank 10 and cleaned above. The liquid drops after cleaning form a liquid level at the bottom of the tank, when the liquid level rises to the middle part of the tank body, the upper water conveying valve is closed, and the stirring paddle 108 at the bottom is opened to 200 rpm. Because the installation direction of stirring rake 108 blade is downward, can be with the liquid of blade top to both sides of below drawing to form the swirl in stirring rake 108 both sides, wash the washing to fermentation cylinder 10 lower part both sides. And simultaneously, a water channel at the lower part is opened, the inputted water flow cleans the wall of the high-side tank under the action of the stirring paddle 108, the water delivery valve is closed after the water flow is washed for 30min, the rotating speed is reduced to 100rpm/min, the waste water is discharged through the liquid outlets 107 at the two sides of the lower end of the tank, the liquid outlet 107 is closed, and the rotating speed is reduced to 50 rpm/min.

(2) And (3) disinfection: opening a high-temperature steam pipeline, introducing 121-180 ℃, sterilizing and disinfecting by 3 kg of steam, and maintaining the stirring speed at 50rpm/min while introducing the steam so that the steam in the tank body is uniformly distributed. The ultrasonic sterilization device 109 on the inner wall of the upper side of the tank body can be opened to assist sterilization at the same time of steam sterilization. And (3) closing the steam pipeline after 30-50 min, discharging water condensed by the steam from liquid outlets 107 at two sides of the bottom of the tank, closing the liquid outlets 107, and cooling the tank body to room temperature.

(3) Feeding: the feed liquid inlet 102 at the top of the fermentation tank 10 is opened, the feed liquid is fed from the feed liquid inlet 102 through a pipeline, and when the liquid level reaches a certain height, the feed is finished, and the valve is closed to stop feeding.

(4) And (3) disinfection: opening a high-temperature steam pipeline, introducing 121-180 ℃, sterilizing and disinfecting the pipeline passing through the material liquid and the material liquid by 3 kg of steam respectively, maintaining the rotating speed of the stirring paddle 108 at 50rpm/min, closing a steam valve after 30-50 min, and cooling the tank body to room temperature.

(5) Ventilating: after the feed liquid is cooled to room temperature, fermented fungi are introduced from the bacterial liquid inlet 101, the stirring speed is increased to 100rpm/min, the bacteria inlet pipeline is closed, and air is introduced into the micro-interface generator 104 from the air inlet 105. The micro-interface generator 104 breaks the air into micro-bubbles with micron scale, and releases the micro-bubbles into the fermentation liquid, so as to increase the mass transfer area of the phase boundary between the oxygen and the fermentation liquid in the fermentation process, make the two phases fully contacted, improve the concentration of dissolved oxygen in the fermentation liquid, improve the fermentation efficiency, and shorten the fermentation time.

(6) Discharging: after fermentation is completed, ventilation is stopped, the liquid outlet 107 is opened to discharge materials, the stirring speed is reduced to 50rpm/min, the material liquid is used for subsequent separation and purification after the materials are completely discharged, the waste gas in the fermentation tank 10 is replaced by air after being stirred for a period of time, and the discharge valve is closed to perform the next circular fermentation process.

The operation and technological parameters in the fermentation process are completely controlled by a control system 20 of a PLC (or a DCS, a PLC and a DCS), and the control system 20 is connected with each sensor on the fermentation tank 10, so that the automatic intelligent control is realized, and the labor cost is saved.

In the above embodiment, the micro-interface generator 104 is single, and the pressure energy of the gas and/or the kinetic energy of the liquid are converted into the surface energy of the bubbles and transferred to the bubbles, so that the bubbles are broken into micro-bubbles with a diameter of 1 μm or more and less than 1mm, and the micro-bubbles are divided into the pneumatic micro-interface generator 104, the hydraulic micro-interface generator 104 and the gas-liquid linkage micro-interface generator 104 according to an energy input mode or a gas-liquid ratio, wherein the pneumatic micro-interface generator 104 is driven by gas, and the input gas amount is much larger than the liquid amount; the hydraulic micro-interface generator 104 is driven by liquid, and the input gas amount is generally smaller than the liquid amount; the gas-liquid linkage type micro-interface generator 104 is driven by gas and liquid at the same time, and the input gas quantity is close to the liquid quantity. The micro-interface generator 104 is selected from one or more of a pneumatic micro-interface generator 104, a hydraulic micro-interface generator 104 and a gas-liquid linkage micro-interface generator 104.

In order to increase the dispersion and mass transfer effects, an additional micro-interface generator 104 can be additionally arranged, the installation position is not limited in fact, the micro-interface generator can be arranged externally or internally, and the micro-interface generator can be arranged on the side wall in the kettle in a relative mode when the micro-interface generator is arranged internally, so that micro-bubbles discharged from the outlet of the micro-interface generator 104 are opposite.

In the above embodiment, the number of the pump bodies is not specifically required, and the pump bodies may be arranged at corresponding positions as required.

In the above embodiment, the fermentation temperature may be normal temperature or temperature required by the strain, the fermentation pressure is normal pressure, and the fermented substance may be penicillin, citric acid, erythromycin, etc.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims (9)

1. A fermentation system, comprising: the fermentation tank is connected with the control system to control the working state of the fermentation tank, a bacterial liquid inlet and a feed liquid inlet are formed in the top of the fermentation tank and used for allowing bacterial liquid and feed liquid to enter, and a liquid outlet is formed in the bottom of the fermentation tank and used for discharging fermentation products;

the fermentation tank is internally provided with a liquid ejector and a micro-interface generator, the micro-interface generator is arranged at the bottom in the fermentation tank, the liquid ejector is arranged at the upper part in the fermentation tank, the side wall of the fermentation tank is provided with a cleaning water inlet and an air inlet, the air inlet is communicated into the micro-interface generator through a pipeline, and the liquid ejector is connected with the cleaning water inlet through a pipeline.

2. The fermentation system of claim 1, wherein the top of the liquid ejector is a semicircular arc surface, a plurality of injection ports are sequentially arranged on the semicircular arc surface, the injection direction of the injection ports faces the top of the fermentation tank, and the cleaning water inlet is connected with the bottom of the liquid ejector through a pipeline.

3. The fermentation system of claim 2, wherein a mesh surface with a plurality of uniformly distributed micropores is laid in each spray opening.

4. The fermentation system of claim 1, wherein the micro-interface generator is disposed opposite the liquid jet.

5. The fermentation system of any one of claims 1 to 4, wherein the bottom of the fermentation tank is flat on two sides, a protrusion is formed at the center of the bottom of the fermentation tank, and the liquid outlets are respectively arranged at the flat positions on two sides of the bottom of the fermentation tank.

6. The fermentation system of claim 5, wherein a stirring paddle is arranged at the central bulge of the bottom in the fermentation tank to accelerate the discharging.

7. The fermentation system of any one of claims 1 to 4, wherein an ultrasonic sterilization device is disposed in the fermentation tank, and the ultrasonic sterilization device is tightly attached to the inner wall of the fermentation tank.

8. The fermentation system according to any one of claims 1 to 4, wherein the number of the washing water inlets provided on the side wall of the fermentation tank is two, one of the washing water inlets is connected to the liquid ejector through a pipe, and the other washing water inlet is provided near the bottom of the fermentation tank.

9. A fermentation process using a fermentation system according to any one of claims 1 to 8, comprising the steps of:

crushing the cleaning water into micron-sized micro-droplets to clean the interior of the fermentation tank;

dispersing and crushing the air micro interface, mixing with a bacterial liquid and a feed liquid, carrying out aerobic fermentation, discharging and collecting a fermentation product.

Images