CN112316178A

CN112316178A - Device and method for disinfecting polluted porous culture plate

Info

Publication number: CN112316178A
Application number: CN202011212196.2A
Authority: CN
Inventors: 浠讳华; 任仪
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-03
Filing date: 2020-11-03
Publication date: 2021-02-05

Abstract

The invention discloses a device and a method for disinfecting a polluted porous culture plate, and the device comprises a bottom plate and a drying box, wherein an ultrasonic cleaning box is arranged above the left side of the bottom plate, liquid inlet pipes are respectively arranged on the outer walls of the left side and the right side of the ultrasonic cleaning box, first moving assemblies are respectively arranged on the edges of the front side and the rear side of the bottom plate, a second moving assembly is connected above the first moving assembly, the drying box is arranged above the right side of the bottom plate, clamping grooves are respectively formed in the middle positions of the upper edges of the front side and the rear side of the drying box, a third moving assembly is arranged on the right side of the drying box, a sealing cover is arranged above the drying box, sealing blocks are respectively arranged on the front side and the rear side of the sealing cover, a flat plate is fixed above the sealing. The culture plate is subjected to primary cleaning, acid soaking overnight, repeated running water fine cleaning, distilled water washing, drying and sterilization to finish the disinfection process, the cleaning effect is good, and the pollution residue is greatly reduced.

Description

Device and method for disinfecting polluted porous culture plate

Technical Field

The invention relates to the technical field of biological tools, in particular to a device and a method for disinfecting a polluted porous culture plate.

Background

Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an indispensable process for both the whole biotechnology and one of the biological cloning technologies, and is a large-scale clone of cells, and the cell culture technology can be a simple single cell or a few differentiated multiple cells through mass culture of one cell, which is an indispensable link of the cloning technology, and is a clone of cells, and a large amount of cells or metabolites thereof are obtained through cell culture, and the biological products are all obtained from the cells, so the cell culture technology can be said to be the most core and basic technology in the biotechnology. The cell culture plate is needed to be used in cell culture, is also called a porous culture plate, is a biological tool, and is needed to be disinfected before cell culture in order to improve the quality of cell culture and avoid the influence of microorganisms carried on the cell culture plate on the result of cell culture.

The porous culture plate on the existing market is when disinfecting, multiunit equipment needs to be used, whole equipment occupies volumetrically, and need the manual work to transport the culture plate between multiunit equipment, lead to the condition of culture plate secondary pollution easily, and current disinfection method exists and can not disinfect to the cell culture plate comprehensively, cell culture plate disinfection quality is not good, the cell culture plate drying efficiency after the disinfection is not high and the obscure problem of bactericidal effect, for this reason, we propose a higher device and disinfection method to polluting the disinfection of porous culture plate of practicality.

Disclosure of Invention

The invention aims to provide a device and a method for disinfecting a polluted porous culture plate, and solves the problems that multiple groups of equipment are needed when the porous culture plate on the market is disinfected, the whole equipment occupies a large volume, the culture plate needs to be manually transported among the multiple groups of equipment, and secondary pollution to the culture plate is easily caused.

In order to achieve the purpose, the invention provides the following technical scheme: a device for disinfecting a polluted porous culture plate comprises a bottom plate and a drying box, wherein an ultrasonic cleaning box is arranged above the left side of the bottom plate, liquid inlet pipes are respectively arranged on the outer walls of the left side and the right side of the ultrasonic cleaning box, first moving assemblies are respectively arranged on the edges of the front side and the rear side of the bottom plate, a second moving assembly is connected above the first moving assembly, the drying box is arranged above the right side of the bottom plate, clamping grooves are respectively formed in the middle positions of the upper edges of the front side and the rear side of the drying box, a third moving assembly is arranged on the right side of the drying box, a sealing cover is arranged above the drying box, sealing blocks are respectively arranged on the front side and the rear side of the sealing cover, a flat plate is fixed above the sealing cover, a support is connected above the flat plate, a draining basket is arranged inside the ultrasonic cleaning box, and connecting rods are respectively, the utility model discloses a drying box, including stoving case, draining basket, ultrasonic cleaning machine case, fan, filter, the inside of draining basket is provided with the cassette, the drain pipe is installed to the bottom intermediate position of ultrasonic cleaning machine case, the safety net is all installed to both sides inner wall around the stoving case, and the outside of safety net is provided with the heating wire, the wind channel has been seted up in the outside of heating wire, the tuber pipe is installed to the bottom intermediate position of stoving case, and the intermediate position of tuber pipe installs the fan, the lower extreme of tuber pipe is connected with the filter, and the.

Preferably, the first movable assembly comprises a screw rod, a servo motor and a rolling sliding table, the servo motor is installed at the left end of the screw rod, the rolling sliding table is connected to the outer side of the screw rod, and meanwhile the rolling sliding table is in threaded connection with the screw rod.

Preferably, the first moving assembly, the second moving assembly and the third moving assembly have the same structure, and the second moving assembly and the third moving assembly are perpendicular to the first moving assembly.

Preferably, the number of the liquid inlet pipes is four, the liquid inlet pipes are communicated with the inside of the ultrasonic cleaning case, and the liquid inlet pipes are distributed in bilateral symmetry about the vertical center line of the ultrasonic cleaning case.

Preferably, the outer surface of the sealing cover and the inner surface of the clamping groove are both connected with sealing gaskets, and the sealing cover forms a sealing structure with the drying box through the sealing block and the clamping groove.

Preferably, the connecting rod and the second moving assembly are fixedly connected, the connecting rod is of an L-shaped structure, and the draining basket is of a hollow mesh structure.

Preferably, the air duct is communicated with the air pipe through a fan, and the air duct is communicated with the inside of the drying box through a safety net.

Preferably, the outer wall of the filter and the inner wall of the air pipe are both provided with threads, the sizes of the threads between the filter and the air pipe are matched, and the filter layer is made of activated carbon.

A sterilization method for sterilizing a contaminated multi-well culture plate, comprising the steps of:

s1, placing the polluted porous culture plate in a draining basket, introducing tap water into an ultrasonic cleaning case, starting the ultrasonic cleaning case for primary cleaning, and discharging sewage;

s2, introducing a disinfectant into the ultrasonic cleaning case, soaking the multi-pore culture plate in the disinfectant overnight, and discharging the disinfectant;

s3, introducing tap water into the ultrasonic cleaning case, starting the ultrasonic cleaning case to clean, discharging sewage, and repeating the step S3 for 3-5 times;

s4, introducing triple distilled water into the ultrasonic cleaning case, starting the ultrasonic cleaning case for cleaning, and discharging sewage;

s5, putting the porous culture plate cleaned in the step S4 into a drying box, covering a sealing cover, and drying;

and S6, finally, irradiating the dried porous culture plate by using an ultraviolet germicidal lamp for 30-60min for sterilization treatment.

Compared with the prior art, the invention has the following beneficial effects:

1. three groups of moving assemblies are arranged and made of the same parts, wherein the first moving assembly is transversely arranged to conveniently drive the second moving assembly to horizontally move so as to complete the transfer of the draining basket between the ultrasonic cleaning case and the drying case, the second moving assembly and the third moving assembly are vertically distributed so as to conveniently complete the lifting of the draining basket and the lifting of the sealing cover, and the three groups of moving assemblies effectively replace manual operation, so that the burden of operators can be reduced, and the possibility of secondary pollution to the culture plate in the transferring process can be reduced;

2. the four liquid inlet pipes are arranged and can be respectively connected with different liquid supply pipelines outside, namely a tap water liquid supply pipeline, a disinfectant liquid supply pipeline, a triple distilled water liquid supply pipeline and other pipelines, so that the cleaning operation with various different effects can be conveniently realized in the ultrasonic cleaning case, and each pipeline supplies liquid separately, so that the condition that the front end mixed pollution of liquid is generated is favorably avoided;

3. according to the invention, the sealing cover and the clamping groove are provided with the sealing gaskets, so that the sealing effect of the sealing cover when the sealing cover covers the drying box can be improved, further, the heat loss in the drying process of the culture plate can be avoided, the overall drying efficiency is higher, and the ultraviolet germicidal lamp on the inner top surface of the sealing cover can completely irradiate the inside of the drying box when in work, so that the sterilization effect of the whole device on the culture plate can be improved;

4. according to the invention, the heat wind is formed to blow to the middle position of the drying box through the matching action of the fan and the electric heating wire in the drying box, so that the culture plate is dried conveniently, the drying efficiency is high, and the air entering the fan can be cleaned and filtered through the filter, so that the pollution of pollutants in the air to the culture plate is reduced;

5. according to the invention, the whole disinfection method is adopted to complete the cleaning process by carrying out primary cleaning on the culture plate, soaking in acid overnight, carrying out flowing water fine cleaning for many times and washing with distilled water, the cleaning effect is good, the pollution residue is greatly reduced, then drying and sterilization are carried out, the disinfection effect on the culture plate is effectively improved, in addition, the mechanical assembly is utilized to complete the transportation of the culture plate in the whole disinfection and cleaning process, the manual operation is reduced, and the reduction of the burden of workers is facilitated.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a left side view of the ultrasonic cleaning cabinet and the second movable assembly of the present invention;

FIG. 3 is a schematic structural view of a left side cross section of the drying box of the present invention;

FIG. 4 is a schematic view of the internal cross-sectional structure of the sealing cap of the present invention;

FIG. 5 is a schematic cross-sectional view of the connection between the air duct and the filter according to the present invention.

In the figure: 1. a base plate; 2. ultrasonically cleaning the chassis; 3. a liquid inlet pipe; 4. a first moving assembly; 401. a screw rod; 402. a servo motor; 403. a rolling sliding table; 5. a second moving assembly; 6. a drying box; 7. a third moving assembly; 8. a support; 9. a flat plate; 10. a sealing cover; 11. a sealing block; 12. a connecting rod; 13. a draining basket; 14. a card holder; 15. a drain pipe; 16. an air duct; 17. an electric heating wire; 18. a safety net; 19. an air duct; 20. a fan; 21. a filter; 22. a metal mesh; 23. a filter layer; 24. an ultraviolet germicidal lamp; 25. a gasket; 26. a clamping groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, a device for sterilizing a contaminated multi-well culture plate comprises a bottom plate 1, an ultrasonic cleaning cabinet 2, a liquid inlet pipe 3, a first moving assembly 4, a screw rod 401, a servo motor 402, a rolling sliding table 403, a second moving assembly 5, a drying box 6, a third moving assembly 7, a bracket 8, a flat plate 9, a sealing cover 10, a sealing block 11, a connecting rod 12, a draining basket 13, a clamping seat 14, a drain pipe 15, an air duct 16, an electric heating wire 17, a safety net 18, an air duct 19, a fan 20, a filter 21, a metal net 22, a filter layer 23, an ultraviolet germicidal lamp 24, a sealing gasket 25 and a clamping groove 26, wherein the ultrasonic cleaning cabinet 2 is installed above the left side of the bottom plate 1, the liquid inlet pipe 3 is installed on the outer walls of the left and right sides of the ultrasonic cleaning cabinet 2, the first moving assembly 4 is installed on the edges of the front and back sides of the bottom plate 1, and the, the drying box 6 is arranged above the right side of the bottom plate 1, the middle positions of the upper edges of the front side and the rear side of the drying box 6 are respectively provided with a clamping groove 26, the right side of the drying box 6 is provided with a third moving component 7, the upper side of the drying box 6 is provided with a sealing cover 10, the front side and the rear side of the sealing cover 10 are respectively provided with a sealing block 11, a flat plate 9 is fixed above the sealing cover 10, a bracket 8 is connected above the flat plate 9, a draining basket 13 is arranged inside the ultrasonic cleaning machine case 2, connecting rods 12 are arranged on the front side and the rear side above the draining basket 13, a clamping seat 14 is arranged inside the draining basket 13, a draining pipe 15 is arranged at the middle position of the bottom of the ultrasonic cleaning machine case 2, safety nets 18 are arranged on the inner walls of the front side and the rear side of the drying box 6, heating wires 17 are arranged on the outer, a fan 20 is arranged in the middle of the air pipe 19, a filter 21 is connected to the lower end of the air pipe 19, a metal mesh 22 is fixed inside the filter 21, a filter layer 23 is arranged inside the metal mesh 22, and an ultraviolet germicidal lamp 24 is arranged inside the sealing cover 10;

the first moving assembly 4 comprises a screw rod 401, a servo motor 402 and a rolling sliding table 403, the servo motor 402 is installed at the left end of the screw rod 401, the rolling sliding table 403 is connected to the outer side of the screw rod 401, meanwhile, the rolling sliding table 403 is in threaded connection with the screw rod 401, the first moving assembly 4, the second moving assembly 5 and the third moving assembly 7 are in the same structure, the second moving assembly 5, the third moving assembly 7 and the first moving assembly 4 are perpendicular to each other, three groups of moving assemblies are arranged and are made of the same parts, wherein the first moving assembly 4 is transversely arranged to conveniently drive the second moving assembly 5 to horizontally move, the draining basket 13 is transferred between the ultrasonic cleaning case 2 and the drying case 6, and the second moving assembly 5 and the third moving assembly 7 are vertically distributed to conveniently complete the lifting of the draining basket 13 and the lifting of the sealing cover 10, the three groups of moving assemblies effectively replace manual operation, the burden of operators can be relieved, and the possibility of secondary pollution to the culture plates in the transferring process can be reduced, the number of the liquid inlet pipes 3 is four, the liquid inlet pipes 3 are communicated with the inside of the ultrasonic cleaning case 2, the liquid inlet pipes 3 are symmetrically distributed in the left and right direction of the vertical central line of the ultrasonic cleaning case 2, the four liquid inlet pipes 3 are respectively connected with different external liquid supply pipelines, namely a tap water liquid supply pipeline, a disinfectant liquid supply pipeline, a triple-distilled water liquid supply pipeline and other pipelines, so that the cleaning operation with various different effects can be realized in the ultrasonic cleaning case 2, and each pipeline supplies liquid separately, and the condition that the front end mixed pollution of the liquid is generated can be avoided;

the outer surface of the sealing cover 10 and the inner surface of the clamping groove 26 are both connected with a sealing gasket 25, the sealing cover 10 forms a sealing structure with the drying box 6 through the sealing block 11 and the clamping groove 26, the connecting mode between the connecting rod 12 and the second moving component 5 is fixed connection, the appearance structure of the connecting rod 12 is an L-shaped structure, the appearance structure of the draining basket 13 is a hollowed-out mesh structure, the sealing gasket 25 is arranged on the sealing cover 10 and the clamping groove 26, the sealing effect of the sealing cover 10 covering the drying box 6 is improved, heat loss in the drying process of the culture plate is avoided, the overall drying efficiency is higher, the ultraviolet germicidal lamp 24 on the inner top surface of the sealing cover 10 can completely irradiate the inside of the drying box 6 when in work, the sterilizing effect of the whole device on the culture plate is improved, and the air duct 16 forms a communicating structure with the air duct 19 through the fan 20, and wind channel 16 is linked together through safety net 18 and stoving case 6 is inside, the outer wall of filter 21 and the inner wall of tuber pipe 19 all set up the screw thread, and the screw thread size between filter 21 and the tuber pipe 19 is identical, and the material of filter layer 23 is active carbon, set up the cooperation that passes through fan 20 and heating wire 17 in the stoving case 6, it blows to stoving case 6 intermediate position to form the hot-blast, so that dry to the cultivation board, drying efficiency is high, and the air that gets into fan 20 inside can be cleaned through filter 21 and filtered, and then be favorable to reducing the pollution of the pollutant in the air to the cultivation board.

s1, placing the polluted porous culture plate in the draining basket 13, introducing tap water into the ultrasonic cleaning case 2, starting the ultrasonic cleaning case 2 for primary cleaning, and discharging sewage;

s2, introducing a disinfectant into the ultrasonic cleaning case 2, soaking the multi-hole culture plate in the disinfectant overnight, and discharging the disinfectant;

s3, introducing tap water into the ultrasonic cleaning case 2, starting the ultrasonic cleaning case 2 to clean, discharging sewage, and repeating the step S3 for 3-5 times;

s4, introducing triple distilled water into the ultrasonic cleaning case 2, starting the ultrasonic cleaning case 2 for cleaning, and discharging sewage;

s5, putting the porous culture plate cleaned in the step S4 into a drying box 6, covering a sealing cover 10, and drying;

s6, finally, irradiating the dried porous culture plate by using the ultraviolet germicidal lamp 24 for 30-60min for sterilization treatment,

set up whole disinfection method through carrying out preliminary washing to the culture plate, steep sour overnight, flowing water fine washing many times, the washing process is accomplished in distilled water washing, and the cleaning performance is good, and the pollution that has significantly reduced remains, dries and disinfects again, has effectively improved the disinfection effect to the culture plate to utilize mechanical assembly to accomplish the transportation of culture plate among the clean process of whole disinfection, reduce manual operation, be favorable to alleviateing workman's burden.

The working principle is as follows: for the device for sterilizing the polluted porous culture plate, firstly, a bottom plate 1 supports the whole device, liquid inlet pipes 3 at different positions are respectively connected with a tap water supply pipeline, a disinfectant liquid supply pipeline and a triple distilled water supply pipeline, culture plates are firstly obliquely inserted into a clamping seat 14 one by one, the culture plates are placed in a draining basket 13, then the liquid inlet pipe 3 connected with the tap water supply pipeline is opened, tap water is injected into an ultrasonic cleaning case 2, the liquid inlet pipe 3 is closed after the culture plates can be completely immersed when the water level reaches a certain height, the ultrasonic cleaning case 2 is started at the moment, the ultrasonic cleaning case 2 carries out ultrasonic cleaning on the culture plates, after the cleaning is finished, a drain pipe 15 is opened to discharge sewage, then the drain pipe 15 is closed, disinfectant liquid is introduced into the ultrasonic cleaning case 2 to immerse the culture plates, after soaking for one night, draining the disinfectant, introducing tap water, starting the ultrasonic cleaning case 2, flushing for three to five times by running water, discharging sewage, introducing triple-distilled water, and performing ultrasonic cleaning;

after the cleaning is finished, the second moving component 5 is started, so that the second moving component 5 drives the connecting rod 12 and the draining basket 13 to rise to a certain height until the draining basket 13 is completely separated from the ultrasonic cleaning machine case 2, then the servo motor 402 in the first moving assembly 4 is started, the servo motor 402 drives the screw rod 401 to rotate, the screw rod 401 drives the rolling sliding table 403 to move rightwards, the rolling sliding table is moved to the position of the drying box 6, then the second moving assembly 5 is operated, so that the second moving assembly 5 drives the connecting rod 12 and the draining basket 13 to descend until the connecting rod 12 is clamped in the clamping groove 26, the draining basket 13 enters the drying box 6, then, the third moving assembly 7 is operated, so that the third moving assembly 7 drives the sealing cover 10 to descend through the bracket 8 and the flat plate 9, the sealing cover 10 covers the drying box 6, the sealing block 11 is clamped in the clamping groove 26, the connecting rod 12 is pressed, and the sealing gasket 25 enhances the tightness of the joint;

then start fan 20 and heating wire 17, fan 20 sucks the air to the inside of tuber pipe 19 through filter 21, the air gets into inside tuber pipe 19 after filtering through metal mesh 22 and filter layer 23, carry the wind channel 16 inside by tuber pipe 19 again, then blow to heating wire 17, heating wire 17 work gives off the heat, make the air current become hot, hot-air blows to waterlogging caused by excessive rainfall basket 13 through safety net 18, carry out drying process to the culture plate, after the drying is accomplished, start sterilamp 24, sterilamp 24 launches the ultraviolet ray and shines on the culture plate of waterlogging caused by excessive rainfall basket 13 inside, carry out germicidal treatment to it, just so accomplish the whole use of device to the disinfection of contaminated porous culture plate.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A device for sterilizing a contaminated multi-well culture plate, comprising a base plate (1) and a drying box (6), characterized in that: an ultrasonic cleaning case (2) is installed above the left side of the bottom plate (1), liquid inlet pipes (3) are installed on the outer walls of the left side and the right side of the ultrasonic cleaning case (2), first moving assemblies (4) are installed on the edges of the front side and the rear side of the bottom plate (1), a second moving assembly (5) is connected above the first moving assembly (4), the drying box (6) is installed above the right side of the bottom plate (1), clamping grooves (26) are formed in the middle positions of the upper edges of the front side and the rear side of the drying box (6), a third moving assembly (7) is arranged on the right side of the drying box (6), a sealing cover (10) is arranged above the drying box (6), sealing blocks (11) are installed on the front side and the rear side of the sealing cover (10), a flat plate (9) is fixed above the sealing cover (10), and a support (8) is connected above the flat, a draining basket (13) is arranged in the ultrasonic cleaning case (2), connecting rods (12) are arranged on the front side and the rear side above the draining basket (13), a clamping seat (14) is arranged in the draining basket (13), a draining pipe (15) is arranged at the middle position of the bottom of the ultrasonic cleaning case (2), safety nets (18) are arranged on the inner walls of the front side and the rear side of the drying case (6), electric heating wires (17) are arranged on the outer sides of the safety nets (18), an air duct (16) is arranged on the outer side of each electric heating wire (17), an air pipe (19) is arranged at the middle position of the bottom of the drying case (6), a fan (20) is arranged at the middle position of the air pipe (19), a filter (21) is connected to the lower end of the air pipe (19), a metal net (22) is fixed in the filter (21), a filtering layer (23) is arranged in the, an ultraviolet germicidal lamp (24) is arranged inside the sealing cover (10).

2. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: first removal subassembly (4) include lead screw (401), servo motor (402) and roll slip table (403), and servo motor (402) are installed to the left end of lead screw (401), and the outside of lead screw (401) is connected with roll slip table (403), and the connected mode between roll slip table (403) and lead screw (401) is threaded connection simultaneously.

3. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: the first moving assembly (4), the second moving assembly (5) and the third moving assembly (7) are identical in structure, and the second moving assembly (5) and the third moving assembly (7) are perpendicular to the first moving assembly (4).

4. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: the quantity of the liquid inlet pipe (3) is set to four, the liquid inlet pipe (3) is communicated with the inside of the ultrasonic cleaning case (2), and the liquid inlet pipe (3) is distributed in a bilateral symmetry mode about the vertical central line of the ultrasonic cleaning case (2).

5. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: the outer surface of the sealing cover (10) and the inner surface of the clamping groove (26) are both connected with sealing gaskets (25), and the sealing cover (10) forms a sealing structure with the drying box (6) through a sealing block (11) and the clamping groove (26).

6. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: the connecting rod (12) and the second moving assembly (5) are fixedly connected, the connecting rod (12) is of an L-shaped structure, and the draining basket (13) is of a hollow-out mesh structure.

7. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: the air duct (16) is communicated with the air pipe (19) through a fan (20), and the air duct (16) is communicated with the inside of the drying box (6) through a safety net (18).

8. An apparatus according to claim 1, wherein the apparatus is adapted to sterilize a contaminated multi-well culture plate by: the outer wall of the filter (21) and the inner wall of the air pipe (19) are both provided with threads, the sizes of the threads between the filter (21) and the air pipe (19) are identical, and the filter layer (23) is made of activated carbon.

9. A method of sterilizing a contaminated multi-well culture plate, comprising the steps of:

s1, placing the polluted porous culture plate in a draining basket (13), introducing tap water into the ultrasonic cleaning case (2), starting the ultrasonic cleaning case (2) for primary cleaning, and draining sewage;

s2, introducing a disinfectant into the ultrasonic cleaning case (2), soaking the multi-hole culture plate in the disinfectant overnight, and discharging the disinfectant;

s3, introducing tap water into the ultrasonic cleaning case (2), starting the ultrasonic cleaning case (2) for cleaning, discharging sewage, and repeating the step S3 for 3-5 times;

s4, introducing triple distilled water into the ultrasonic cleaning case (2), starting the ultrasonic cleaning case (2) for cleaning, and discharging sewage;

s5, putting the porous culture plate cleaned in the step S4 into a drying box (6), covering a sealing cover (10), and drying;

s6, finally, irradiating the dried porous culture plate by using an ultraviolet germicidal lamp (24) for 30-60min for sterilization treatment.