CN112314484A - Shrimp egg in-vitro hatching device and using method thereof - Google Patents
Shrimp egg in-vitro hatching device and using method thereof Download PDFInfo
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- CN112314484A CN112314484A CN202011186532.0A CN202011186532A CN112314484A CN 112314484 A CN112314484 A CN 112314484A CN 202011186532 A CN202011186532 A CN 202011186532A CN 112314484 A CN112314484 A CN 112314484A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a shrimp egg in-vitro hatching device and a using method thereof, and the shrimp egg in-vitro hatching device comprises a rack, an upper water tank, a lower water tank, a net bag, a water bucket and a submersible pump, wherein the two ends of the lower water tank are respectively communicated with a water inlet and a water outlet; set up the basin in order to set for range and frequency reciprocal horizontal rectilinear movement that makes a round trip in the basin down, the swing action of simulation female shrimp armful of eggs in-process appendage that can be scientific prevents effectively that the oxygen deficiency from leading to the shrimp ovum to die sets up a plurality of string bag matrix arrangements in the basin of going up, is convenient for collect and hierarchical management the shrimp ovum of new rupture of membranes.
Description
Technical Field
The invention relates to the technical field of shrimp egg breeding, in particular to a shrimp egg in-vitro hatching device and a using method thereof.
Background
At present, the breeding of the red swamp crayfish mostly adopts the traditional female crayfish spawning natural hatching, which has the following defects:
(1) the shrimp larvae are difficult to collect, no matter the pond, the cement pond or the net cage, the shrimp larvae are collected and all consume manpower very much, and the loss is big:
(2) the shrimp fries are irregular, so that the phenomena of big deception and eating of young shrimps after shelling easily occur, and temporary rearing and large transportation loss are caused;
(3) bacterial and fungal infection and parasitic diseases are difficult to control;
(4) the close breeding can not be avoided, and the quality of the variety is degraded.
Although similar patent technologies in the prior art include procambarus clarkii egg ex vivo cultivation method, exosomatic cultivation technology of fertilized eggs of exosomatic white shrimps, exosomatic cultivation technology of eggs of red crayfish, etc., these technologies also generally have the following disadvantages:
(1) the classification of shrimp eggs is inaccurate, a large error exists when the development degree of the eggs is classified only by naked eyes, the time for completing the development of the shrimp larvae is probably separated by about 7 days, and the shrimp larvae are extremely not beneficial to the collection and temporary culture of the shrimp larvae;
(2) fertilized eggs are placed in a hatching container and are subjected to oxygen increasing and hatching through water flow or air flow, the risk of water mold infection is high, dead fertilized eggs cannot be picked out in time in the hatching process, or the operation difficulty of picking out dead fertilized eggs is high, the workload is high, and water mold infection can be further caused to cause hatching failure.
Disclosure of Invention
In view of the above, the invention provides the shrimp egg in-vitro hatching device and the use method thereof, wherein the shrimp egg in-vitro hatching device has less death of shrimp eggs, is convenient for the graded management of the shrimp eggs, can effectively control the infection of saprolegnia, and is convenient for accurately judging the growth condition of shrimp seedlings.
The technical scheme of the invention is realized as follows: the invention provides a shrimp egg in-vitro hatching device which comprises a rack, an upper water tank, a lower water tank, a net bag, a water bucket and a submersible pump, wherein the upper water tank is arranged on the rack; the lower water tank is horizontally and fixedly arranged on the frame, and two ends of the lower water tank are respectively communicated with a water inlet and a water outlet; the upper water tank is nested in the lower water tank, the upper water tank moves linearly and horizontally in the lower water tank in a reciprocating manner at a fixed frequency, and a gap is reserved between the bottom of the upper water tank and the bottom surface of the lower water tank; the net bags are fixedly nested on the bottom surface of the upper water tank in a matrix arrangement mode, the net bags are positioned in a gap between the bottom of the upper water tank and the bottom surface of the lower water tank, and the bottoms of the net bags are not in contact with the bottom surface of the lower water tank; the water barrel is fixedly arranged at one end of the frame, and the barrel opening is positioned right below the water outlet; the immersible pump is fixed to be set up in the cask, and the immersible pump communicates to the water inlet.
On the basis of the technical scheme, preferably, all shrimp eggs of only one shrimp are contained in each net bag.
On the basis of the technical scheme, the automatic water feeding device is preferred to further comprise rollers, at least two groups of rollers are symmetrically connected to two sides of the upper water tank in an axial mode, and the roller surfaces are tightly attached to the top end surfaces of the side walls of the lower water tank.
Still further preferably, the top end surfaces of the side walls at two sides of the lower water tank are symmetrically provided with a groove, and each roller is correspondingly positioned in one of the grooves.
Still further preferably, the groove is arcuately concave.
Still further preferably, the table further comprises a table plate and a transmission assembly, wherein the transmission assembly comprises a support, a connecting rod, a rotating disc and a motor; the bedplate is horizontally and fixedly connected with one end of the upper surface of the lower water tank and is close to the water inlet; the motor is fixedly arranged on the bedplate, the rotary table is connected to the output shaft of the motor in a shaft mode, the support is fixedly connected to the top of the upper water tank, and two ends of the connecting rod are respectively connected to the top of the support and the edge of the surface of the rotary table in a shaft mode.
On the basis of the technical scheme, preferably, the water inlet is higher than the water storage surface of the lower water tank, and the water outlet is an overflow outlet.
On the basis of the technical scheme, preferably, the horizontal section of the net bag is square.
On above technical scheme's basis, preferred, still including sled the subassembly, sled the subassembly and include cam, baffle and handle, cam base circle coupling in the lateral wall of frame one end and be close to the water inlet, baffle fixed connection in the lateral wall of basin one end under and be close to the water inlet, the baffle bottom is hugged closely in the cam wheel face, handle fixed connection in cam base circle.
In another aspect, a method for using the shrimp egg hatching device in vitro is provided, which comprises the following steps,
s1, after the water is stored in the water discharge tank and the water bucket, the submersible pump is started, and the water circulation between the water discharge tank and the water bucket is always kept;
s2, gently scraping the shrimp eggs by using stainless steel forceps, and independently placing each egg of the female shrimps into a net bag;
s3, orderly arranging the net bags filled with the shrimp eggs, then placing the net bags into the upper water tank, and locking the net bags to ensure that the shrimp eggs in the net bags are completely immersed in the stored water in the lower water tank;
s4, starting a motor to drive the upper water tank to move back and forth in the lower water tank in a horizontal straight line with a set amplitude and frequency so as to simulate the swing action of the appendages in the egg-taking process of the shrimps;
s5, in the hatching process, the water temperature and the water quality are intelligently controlled in the whole process, and in cooperation with manual management, the net bags containing the shrimp eggs with the new broken membranes are collected and arranged in one area in the upper water tank every day to carry out grading management, so that the shrimp seedlings with completely consistent development degree are obtained;
s6, the net bag containing the developed shrimp seeds is moved out of the water feeding tank every day and is placed into a temporary culture pond or a standard seed culture pond for subsequent culture.
Compared with the prior art, the shrimp egg in-vitro hatching device and the using method thereof have the following beneficial effects:
(1) the upper water tank is arranged to reciprocate horizontally and linearly in the lower water tank with set amplitude and frequency, swing actions of appendages in the process of egg-holding of the female shrimps can be scientifically simulated, the shrimp eggs are effectively prevented from dying due to oxygen deficiency, and the hatching success rate is greatly improved.
(2) The ovum that sets up each female shrimp is placed in a string bag alone, and the back and forth movement along with the basin in the hatching process can make the shrimp ovum in the string bag constantly turn to prevent the shrimp ovum adhesion of each other, and conveniently choose out the dead ovum, thereby effective control water mould infection.
(3) Set up a plurality of string bag matrix arrangement in last basin, be convenient for collect and hierarchical management the shrimp egg of new rupture of membranes to can be more accurate calculate the molting time when shrimp seedling later stage is raised temporarily, the at utmost avoids the molting loss, uses the string bag to also be convenient for carry out the efficient and receives the seedling operation simultaneously, very big saving the cost of labor, and the shrimp seedling loss is low, and is clean neat, and the size is unanimous, and it is very convenient to weigh the count.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a front cross-sectional view of an incubation device of the present invention;
FIG. 2 is a front view of the water supply trough of the present invention;
FIG. 3 is a top view of an incubation device of the present invention;
fig. 4 is a right side view of the pry assembly of the present invention.
In the figure: 1. a frame; 2. a water feeding groove; 3. a water discharging tank; 31. a water inlet; 32. a water outlet; 33. a groove; 4. a platen; 5. a roller; 6. a net bag; 7. a transmission assembly; 71. a support; 72. a connecting rod; 73. a turntable; 74. a motor; 8. a water bucket; 9. a submersible pump; 10. prying the assembly; 101. a cam; 102. a baffle plate; 103. a handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in figure 1, the shrimp egg in-vitro hatching device comprises a rack 1, an upper water tank 2, a lower water tank 3, a bedplate 4, rollers 5, a net bag 6, a transmission assembly 7, a water barrel 8, a submersible pump 9 and a prying assembly 10.
Wherein, the lower water tank 3 is horizontally and fixedly arranged on the frame 1, and two ends of the lower water tank 3 are respectively communicated with a water inlet 31 and a water outlet 32. Wherein, preferably, the water inlet 31 is higher than the water storage surface of the lower water tank 3, and the water outlet 32 is an overflow outlet, so as to form a flowing water body in the lower water tank 3, which helps to prevent shrimp eggs from adhering to each other.
Go up basin 2 nestification in basin 3 down, go up basin 2 and make a round trip reciprocal straight line horizontal migration with fixed frequency in basin 3 down, go up and leave the clearance between 2 bottoms of basin and the 3 tank bottoms faces of basin down, the swing action of simulation female shrimp armful egg in-process appendage that can be scientific effectively prevents that the oxygen deficiency from leading to the shrimp ovum to die, improves the hatching success rate greatly.
Fixed nestification in 2 tank bottoms of last basin of 6 matrix arrangements of string bag, it is used for holding the shrimp ovum, and string bag 6 is located the clearance between 2 bottoms of last basin and 3 tank bottoms of lower basin, and 6 bottoms of string bag do not contact 3 tank bottoms of lower basin. It should be noted that the mesh size of the net 6 should be smaller than the size of a single shrimp egg. Wherein, only hold whole shrimp eggs of a shrimp in every string bag 6 to guarantee that the hatching of shrimp egg is unanimous with rupture of membranes time, help the growth situation of shrimp egg to carry out unified control and management, also be convenient for simultaneously receive the seedling.
The water barrel 8 is fixedly arranged at one end of the frame 1, and the barrel opening is positioned right below the water outlet 32 to provide water storage.
The submersible pump 9 is fixedly arranged in the water barrel 8, and the submersible pump 9 is communicated to the water inlet 31, so that water circulation is always kept between the lower water tank 3 and the water barrel 8.
Specifically, the invention is also realized by the following technical scheme.
As shown in fig. 1, with reference to fig. 2 and 3, at least two sets of rollers 5 are symmetrically and axially connected to two sides of the upper water tank 2, the surfaces of the rollers 5 are tightly attached to the top end surface of the side wall of the lower water tank 3, and the upper water tank 2 is erected in the lower water tank 3 through the rollers 5.
The bedplate 4 is horizontally and fixedly connected with one end of the upper surface of the lower water tank 3 and close to the water inlet 31 and is used for installing a motor 74.
The transmission assembly 7 comprises a support 71, a connecting rod 72, a rotary disc 73 and a motor 74, the motor 74 is fixedly installed on the bedplate 4, the rotary disc 73 is coupled to an output shaft of the motor 74, the support 71 is fixedly connected to the top of the upper water tank 2, and two ends of the connecting rod 72 are respectively coupled to the top of the support 71 and the disc surface edge of the rotary disc 73.
When the technical scheme is adopted, the motor 74 drives the rotary disc 73 to rotate, and one end of the connecting rod 72 is connected to the edge of the rotary disc 73 in a shaft mode, so that when the rotary disc 73 rotates, the connecting rod 72 can be pulled and pushed alternately in sequence, the support 71 can be pulled and pushed, and the reciprocating horizontal linear motion of the upper water tank 2 is further realized.
In addition, as shown in fig. 1 and with reference to fig. 4, the prying assembly 10 includes a cam 101, a baffle 102 and a handle 103, wherein a base circle of the cam 101 is coupled to a side wall of one end of the frame 1 and is close to the water inlet 31, the baffle 102 is fixedly connected to a side wall of one end of the lower water tank 3 and is close to the water inlet 31, a bottom of the baffle 102 is tightly attached to a wheel surface of the cam 101, and the handle 103 is fixedly connected to a base circle of the cam 101.
When the technical scheme is adopted, the handle 103 is pressed downwards to enable the cam 101 to rotate, so that the small circle of the cam 101 pushes the baffle plate 102, and one end of the lower water tank 3 is tilted, and therefore the stored water in the tank can be discharged from the water outlet 32 to clean the device. It should be noted that, in order to avoid the inclined launching chute 3 from sliding off the rack 1 when one end of the launching chute 3 is tilted, the other ends of the launching chute 3 and the rack 1 may be hinged.
In order to limit the moving range of the rollers 5, grooves 33 are symmetrically formed in the top end surfaces of the side walls of the two sides of the lower water tank 3, and each roller 5 is correspondingly positioned in one groove 33. Preferably, the groove 33 is arc-shaped and concave, so that the upper water tank 2 can swing like a cradle when reciprocating, so as to simulate swing of the appendage in the process of spawning of the female shrimps more scientifically.
As an alternative embodiment, the net bags 6 are square in horizontal section, so that the adjacent net bags 6 can be tightly attached to each other, and the accommodating area of the upper water tank 2 is utilized to the maximum extent.
In another aspect, a method of using the same, comprising the steps of,
s1, after the water is stored in the lower water tank 3 and the water bucket 8, the submersible pump 9 is started, and the water circulation between the lower water tank 3 and the water bucket 8 is always kept;
s2, gently scraping the shrimp eggs by using stainless steel forceps, and independently placing each egg of the female shrimps into a net bag 6;
s3, orderly arranging the net bags 6 filled with the shrimp eggs, then placing the net bags 6 into the upper water tank 2, and locking the net bags 6 to ensure that the shrimp eggs in the net bags 6 are completely immersed in the water storage of the lower water tank 3;
s4 starts the motor 74 to drive the upper water tank 2 to move back and forth in the lower water tank 3 in a reciprocating horizontal straight line with a set amplitude and frequency so as to simulate the swing action of the appendages in the egg-taking process of the female shrimps, so that the death of the shrimp eggs caused by oxygen deficiency can be effectively prevented, and the hatching success rate is greatly improved;
s5, in the hatching process, the water temperature and the water quality are intelligently controlled in the whole process, and in cooperation with manual management, the net bags 6 containing the shrimp eggs with the new broken membranes are collected and arranged in one of the areas in the upper water tank 2 every day for hierarchical management, so that the shrimp seeds with completely consistent development degree are obtained, the shelling time of the shrimp seeds during later temporary rearing can be accurately calculated, and shelling loss is avoided to the maximum extent; it should be noted that, in the hatching process, the temperature of the water in the lower water tank 3 can be automatically and intelligently controlled to be kept at about 26.5 ℃ through a control system and a sensor so as to simulate an ideal temperature environment for hatching the shrimp eggs, and the oxygen flow rate for storing water in the lower water tank 3 is controlled so as to avoid breeding of germs, wherein each tuck net 6 needs to be regularly inspected in manual management, and dead eggs in the tuck net are picked up;
s6, the net bag 6 containing the developed shrimp seeds is moved out of the upper water tank 2 every day and is placed into a temporary culture pond or a standard seed culture pond for subsequent culture.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The utility model provides a shrimp egg hatching apparatus in vitro which characterized in that: comprises a frame (1), an upper water tank (2), a lower water tank (3), a net bag (6), a water bucket (8) and a submersible pump (9);
the lower water tank (3) is horizontally and fixedly arranged on the rack (1), and two ends of the lower water tank (3) are respectively communicated with a water inlet (31) and a water outlet (32);
the upper water tank (2) is nested in the lower water tank (3), the upper water tank (2) moves back and forth linearly and horizontally in the lower water tank (3) at a fixed frequency, and a gap is reserved between the bottom of the upper water tank (2) and the bottom surface of the lower water tank (3);
the net bags (6) are fixedly nested on the bottom surface of the upper water tank (2) in a matrix arrangement manner, the net bags (6) are positioned in a gap between the bottom of the upper water tank (2) and the bottom surface of the lower water tank (3), and the bottoms of the net bags (6) are not in contact with the bottom surface of the lower water tank (3);
the bucket (8) is fixedly arranged at one end of the rack (1), and a bucket opening is positioned right below the water outlet (32);
the submersible pump (9) is fixedly arranged in the water barrel (8), and the submersible pump (9) is communicated to the water inlet (31).
2. The shrimp egg hatching apparatus of claim 1 wherein: all shrimp eggs of only one shrimp are contained in each net bag (6).
3. The shrimp egg hatching apparatus of claim 1 wherein: the water tank is characterized by further comprising at least two groups of rollers (5), wherein the rollers (5) are symmetrically and axially connected to two sides of the upper water tank (2), and the wheel surfaces of the rollers (5) are tightly attached to the top end surface of the side wall of the lower water tank (3).
4. The shrimp egg hatching apparatus of claim 3 wherein: the top end surfaces of the side walls of the two sides of the water discharging groove (3) are symmetrically provided with grooves (33), and each roller (5) is correspondingly positioned in one of the grooves (33).
5. The shrimp egg hatching device in vitro of claim 4, wherein: the groove (33) is arc-shaped and concave.
6. The shrimp egg hatching apparatus of claim 3 wherein: the table plate (4) and the transmission assembly (7) are further included, and the transmission assembly (7) comprises a support (71), a connecting rod (72), a rotary disc (73) and a motor (74);
the bedplate (4) is horizontally and fixedly connected to one end of the upper surface of the lower water tank (3) and is close to the water inlet (31);
the motor (74) is fixedly arranged on the bedplate (4), the turntable (73) is coupled to an output shaft of the motor (74), the bracket (71) is fixedly connected to the top of the upper water tank (2), and two ends of the connecting rod (72) are respectively coupled to the top of the bracket (71) and the edge of the surface of the turntable (73).
7. The shrimp egg hatching apparatus of claim 1 wherein: the water inlet (31) is higher than the water storage surface of the lower water tank (3), and the water outlet (32) is an overflow outlet.
8. The shrimp egg hatching apparatus of claim 1 wherein: the horizontal section of the net bag (6) is square.
9. The shrimp egg hatching apparatus of claim 1 wherein: still move subassembly (10) including prizing, prizing subassembly (10) and including cam (101), baffle (102) and handle (103), cam (101) base circle coupling just is close to water inlet (31) in the lateral wall of frame (1) one end, baffle (102) fixed connection just is close to water inlet (31) in the lateral wall of basin (3) one end down, baffle (102) bottom is hugged closely in cam (101) wheel face, handle (103) fixed connection is in cam (101) base circle.
10. The using method of the shrimp egg in-vitro hatching device is characterized in that: comprises the following steps of (a) carrying out,
s1, after the water is stored in the lower water tank (3) and the water bucket (8), starting the submersible pump (9), and always keeping the water flow circulation between the lower water tank (3) and the water bucket (8);
s2, gently scraping the shrimp eggs by using stainless steel tweezers, and independently placing each egg of the female shrimps into one net bag (6);
s3, the net bags (6) with the separated shrimp eggs are arranged in order and then placed in the upper water tank (2), the net bags (6) are locked, and the shrimp eggs in the net bags (6) are completely immersed in the water storage of the lower water tank (3);
s4, the motor (74) is started to drive the upper water tank (2) to reciprocate horizontally and linearly in the lower water tank (3) at a set amplitude and frequency so as to simulate the swing action of the appendages in the process of hatching the female shrimps;
s5, in the hatching process, the water temperature and the water quality are intelligently controlled in the whole process, and the net bags (6) containing the shrimp eggs with the new broken membranes are collected in one area arranged in the upper water tank (2) every day to carry out grading management by matching with manual management, so that the shrimp seedlings with completely consistent development degree are obtained;
s6, the net bag (6) containing the developed shrimp seeds is moved out of the water feeding tank (2) every day and is placed into a temporary culture pond or a standard seedling pond for subsequent culture.
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| CN202011186532.0A CN112314484A (en) | 2020-10-30 | 2020-10-30 | Shrimp egg in-vitro hatching device and using method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115443929A (en) * | 2022-10-17 | 2022-12-09 | 广西壮族自治区水产技术推广站 | Freshwater crayfish fertilized egg incubator and use method thereof |
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Application publication date: 20210205 |
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