CN112305313A - Plant leaf impedance spectrum measuring device and method based on AD5933 chip - Google Patents
Plant leaf impedance spectrum measuring device and method based on AD5933 chip Download PDFInfo
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- CN112305313A CN112305313A CN202011176771.8A CN202011176771A CN112305313A CN 112305313 A CN112305313 A CN 112305313A CN 202011176771 A CN202011176771 A CN 202011176771A CN 112305313 A CN112305313 A CN 112305313A
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- 238000001453 impedance spectrum Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 32
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000000853 adhesive Substances 0.000 claims abstract description 17
- 230000001070 adhesive effect Effects 0.000 claims abstract description 17
- 229910052709 silver Inorganic materials 0.000 claims abstract description 10
- 239000004332 silver Substances 0.000 claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims description 63
- 238000001566 impedance spectroscopy Methods 0.000 claims description 6
- 239000003973 paint Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims 2
- 238000005259 measurement Methods 0.000 abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 5
- 238000005070 sampling Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000002591 computed tomography Methods 0.000 description 2
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- 238000001035 drying Methods 0.000 description 2
- 230000005251 gamma ray Effects 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000001225 nuclear magnetic resonance method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
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- 230000001766 physiological effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R27/00—Arrangements for measuring resistance, reactance, impedance, or electric characteristics derived therefrom
- G01R27/02—Measuring real or complex resistance, reactance, impedance, or other two-pole characteristics derived therefrom, e.g. time constant
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
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Abstract
The invention discloses a plant leaf impedance spectrum measuring device and method based on an AD5933 chip, and belongs to the technical field of impedance spectrum measurement. The plant leaf impedance spectrum measuring device based on the AD5933 chip comprises: the bracket is in a shape like a Chinese character 'tu', one end of the bracket is opened, and the two ends of the opening are respectively wound on the rubber rod; one end of each insulating tube is sleeved outside one end of the rubber rod, silver wires penetrate through the rubber rod and extend out of two ends of the rubber rod, and conductive adhesive is filled in the insulating tubes; the other ends of the two rubber rods are respectively connected with an AD5933 chip electrode clamp through silver wires at the port. The impedance spectrum measuring device measures the impedance spectrum of the plant leaf by unifying the clamping force and fixing the measuring area, has high possibility of measuring the repeatability of the same position, and can accurately construct the impedance spectrum model of the plant leaf.
Description
Technical Field
The invention belongs to the technical field of impedance spectroscopy measurement, and particularly relates to a plant leaf impedance spectroscopy measurement device and a plant leaf impedance spectroscopy measurement method based on an AD5933 chip.
Background
Water is an important basis for plant metabolism and is essential for plant survival. The water content of the plant stem is more closely related to the efficiency of the plant in transporting substances, the efficiency of photosynthesis and the growth condition of the plant. At present, a great deal of research on the moisture content of plant stems faces an important technical bottleneck, namely, a convenient, nondestructive, visual, accurate and cheap plant moisture content measuring device is lacked.
At present, the most direct, most common and most representative plant water content determination method in China is a drying method, and a sample weighing method is also adopted. The operation process is that the plant stem is directly sampled, the weight of the obtained isolated branch segment is weighed and then put into an oven to be dried until the quality is not changed, namely the dry weight is obtained, and the water content can be measured. However, this method has the advantage of being very accurate, intuitive and simple. The method has the prominent disadvantage that the method has great damage to the plant, particularly the stem part which is wanted to be researched is a key part of the plant, and the physiological activity of the plant is greatly influenced. Moreover, the great loss of water at the cut can cause great influence on the accuracy of the experimental result. In addition, different parts of the plant have large water content difference, and the drying method cannot realize real-time measurement and accurate measurement.
And the non-destructive real-time online detection of the water content of the plant stem body leads foreign scholars to start earlier and adopts a plurality of indirect means. Such as gamma ray methods, nuclear magnetic resonance methods, computed tomography techniques and resistivity methods. Among the methods, the gamma ray method has potential safety hazards, the nuclear magnetic resonance method and the computer tomography technology have high cost and very high requirements on the use environment, the realization degree and the convenience degree of the method are greatly limited, and the field fixed-point long-term monitoring can not be realized, so the method can not be widely applied and popularized. When the water content of the living arbor is measured by the resistance method, the huge defect that the absolute water content and the resistance value are not single-valued functions exists. These methods also do not solve the problems encountered at the present stage.
Similarly, the theoretical perfection and technical realization of the technology, which is a technology for accurately controlling irrigation according to plant requirements and the like in the future development trend of water-saving irrigation technology, are also limited by the technology. Based on the impedance spectrum module, the method for measuring the water content of the plant stem is researched, and the water content data of the plant stem can be conveniently and accurately measured in real time on the basis of not damaging the physiological structure of the plant, so that the method is used for researching the growth condition, the physiological characteristics and the like of the plant.
The main problem when measuring the impedance spectrum of the plant at present is that the repeatability is poor, even if the same sensor repeatedly measures the leaf result in the same state, the result still has great difference, so although the moisture content of the plant changes, the impedance spectrum changes remarkably, the measured value is a relative value, the normalization is difficult, and the measured result is difficult to compare. The reason for this is that the blade surface is not flat and cannot be machined, and the clamping force applied by the electrode in each measurement is different, which not only easily causes blade damage, but also causes different actual contact areas and different contact resistances, thereby causing deviation of results. In order to accurately compare the physiological impedance of plants, flexible materials are used for contacting the leaf, and the contact area and the contact impedance of the fixed electrode are urgent prerequisites of current plant electrophysiological research.
Disclosure of Invention
The invention aims to solve the defects of the prior art and provide an AD5933 chip-based plant leaf impedance spectrum measuring device and a measuring method, the impedance spectrum measuring device overcomes the uncertainty of the metal electrode contacting the leaf by using a conductive adhesive to contact the leaf, fixing a measuring area and contact resistance, and the measured impedance spectrum of the plant leaf has good repeatability, so that the impedance spectrum values of different leaves of different tree species have comparability, and the impedance spectrum measuring device can be used for accurately constructing a plant leaf impedance spectrum model.
The invention adopts the following technical scheme:
the invention provides a plant leaf impedance spectrum measuring device based on an AD5933 chip, which comprises:
the bracket is in a shape like a Chinese character 'tu', one end of the bracket is opened, and the two ends of the opening are respectively wound on the rubber rod;
one end of each insulating rubber tube is sleeved outside one end of the rubber rod, and the other end of each insulating rubber tube presses the blade during measurement; the silver wire penetrates through the middle of the rubber rod and extends out of two ends of the rubber rod, and the rubber rod is used as an insulating airtight seal to seal conductive adhesive in the insulating rubber tube. One end of the silver wire is in contact with the conductive adhesive, and the other end of the silver wire is connected with the impedance spectrum measuring circuit;
the contact surfaces of the two insulating rubber tubes pressing to the blades are completely the same and are aligned up and down, the two insulating rubber tubes are pressed against each other by an elastic oc-shaped bracket, and the cavities of the insulating rubber tubes are filled with conductive adhesive. The conductive adhesive is used as an electrode for connecting the blade and the measuring device, is in liquid state and is in close contact with the surface of the blade, so that the contact resistance and the actual contact area are constant.
And the other ends of the two rubber rods are respectively connected with an AD5933 chip electrode clamp through silver wires at ports.
Furthermore, the oc-shaped bracket is a spring wire, and two ends of the oc-shaped bracket are respectively spirally wound on the rubber rod.
Furthermore, the surface of the part of the oc-shaped bracket wound on the rubber rod is coated with paint so as to insulate the part.
Furthermore, the insulating tube is an insulating rubber tube, and the rubber tube is cylindrical.
Furthermore, the ^ shaped bracket is made of spring wires.
The invention provides a method for measuring an impedance spectrum of a plant leaf by using a plant leaf impedance spectrum measuring device based on an AD5933 chip, which comprises the following steps:
the method comprises the following steps: selecting fresh branches of plants to be detected growing in different environments with leaves, and wrapping the base parts of the branches;
step two: cleaning leaves on the fresh branches, and picking the leaves with more consistent growth vigor;
step three: the leaf is clamped between two insulating pipes of the measuring device, and the measuring voltage and frequency are set through arduino software to measure the plant impedance spectrum.
The spring wire is bent into a small tweezer shape (the small tweezer spring wire is a bracket which is in a shape of being a mark or a mark in the description), the open end of the spring wire is wrapped with paint to insulate the spring wire so as not to influence the experimental result, and the elasticity of the spring wire generates acting force to enable the two ends of the device to be attached to the leaves more tightly, so that the possible experimental error is reduced; the rubber rod wound by the spring wire is wrapped by an insulated rubber tube, so that the silver wires at two ends can be fixed; and the silver wires at the two ends are bent, the contact area with the conductive adhesive is increased, electrodes are led out (the silver wires are inserted into the rubber rod, the two ends are led out to ensure the tightness), and meanwhile, the conductive adhesive is injected into the rubber tube to ensure the good conductivity of the device.
The invention uses the rubber tube made of insulating material, fixes the rubber tube on two sides of the leaf by a clamp in a shape of a size of a half, the conductive adhesive is poured in the rubber tube, and the opening is sealed by the sealing head with the silver wire electrode.
The resistances of the silver wire and the conductive adhesive are very small in the whole frequency spectrum range and basically unchanged. The design of the electrode enables the impedance spectrum measuring result to well reflect the water content change of the plant leaves, and the problem of poor repeated stability is well solved. According to the invention, a circuit module based on an AD5933 chip is built to replace a heavy impedance meter, so that the miniaturization of the measuring device can be well realized, and the living body measurement of the moisture content of the plant can be finally realized.
Compared with the prior art, the invention has the beneficial effects that:
firstly, the method comprises the following steps: according to the invention, the contact resistance and the actual contact area are stable through the contact of the conductive adhesive and the blade, so that the contact of the metal electrode and the blade and the uncertainty of the actual contact area and the contact resistance are thoroughly overcome;
secondly, the method comprises the following steps: according to the invention, the rubber tube is selected to contact the blade, and the conductive adhesive is restrained, so that the measurement area is fixed;
thirdly, the method comprises the following steps: according to the invention, the insulating rubber tube wraps the conductive adhesive to contact the blades, the conductive adhesive is liquid, and the rubber tube is made of flexible material, so that the plant blades cannot be damaged during measurement, the impedance of the plant blades with different thicknesses can be nondestructively detected on line, and the structure of the device is simplified;
fourthly: the method has the advantages that the repeatability of the measurement result is good, the comparability is realized, the moisture content of the plant leaves can be conveniently and quantitatively tested, and the impedance performance of the plant leaves can be quantitatively judged;
fifth, the method comprises the following steps: on the premise of not damaging the physiological structure of the plant, the impedance spectrum of the plant stem is researched in a large quantity so as to conveniently and accurately measure the water content data of the plant stem in real time;
sixth: the invention is simple, accurate and high in precision, and the measuring result is not influenced by the determined conditions, thus having universality.
Drawings
FIG. 1 is a schematic structural diagram of a plant leaf impedance spectroscopy device based on an AD5933 chip according to the present invention;
FIG. 2 is a schematic view showing the structure of an insulating tube of the apparatus for measuring impedance spectrum of plant leaves according to the present invention;
FIG. 3 is a scatter plot of plant stem impedance spectrum data measured at various time points using the apparatus of the present invention;
reference numerals: 1. a oc-shaped stent; 2. an insulating tube; 3. a rubber rod; 4. silver wire; 5. and (3) conductive adhesive.
Detailed Description
In order that the invention may be more fully understood, reference will now be made to the following description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Examples
As can be seen from fig. 1 to 2, the plant leaf impedance spectroscopy apparatus based on the AD5933 chip of the present invention includes:
the support comprises a oc-shaped spring wire support 1, wherein one end of the oc-shaped support 1 is opened, two ends of the opening are spirally wound on a rubber rod 3 respectively, and paint is coated on the surface of a part, wound on an insulating rod 3, of the oc-shaped support 1 so as to insulate the part;
one end of each insulating rubber tube 2 is sleeved at one end of the rubber rod 3, a silver wire 4 penetrates through the rubber rod 3, the silver wires 4 extend out of two ends of the rubber rod 3, conductive adhesive 5 is filled in the insulating rubber tubes 2, and the insulating rubber tubes are cylindrical;
the other ends of the two rubber rods 3 are respectively connected with an AD5933 chip electrode clamp through silver wires at the port.
The invention provides a method for measuring an impedance spectrum of a plant leaf by using a plant leaf impedance spectrum measuring device based on an AD5933 chip, which comprises the following steps:
the method comprises the following steps: selecting fresh branches of plants to be detected growing in different environments with leaves, and wrapping the base parts of the branches;
step two: cleaning leaves on the fresh branches, and picking the leaves with more consistent growth vigor;
step three: the leaf is clamped between two insulating pipes 2 of the measuring device, and the measuring voltage and frequency are set through arduino software to measure the plant impedance spectrum.
Plant stem impedance spectrum data are measured through the experimental device. The experimental data are shown in table 1, table 2 and fig. 3.
TABLE 1 impedance spectrum data of plant stems tested at different time points
TABLE 2 impedance spectrum data of plant stems tested at different time points
It can be seen from tables 1 and 2 that experimental data are obtained continuously through repeated tests, and a large amount of experimental data are analyzed and processed, we find that, aiming at the continuously generated burr phenomenon in the obtained data, in addition to eliminating an extreme value, we can enlarge sampling intervals by reducing and dispersing sampling points, and sharply reduce burrs in a mode of repeatedly sampling a single frequency to ensure the overall density and integrity of the data.
The embodiments of the present invention have been described in detail with reference to the above examples, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (6)
1. Plant leaf impedance spectroscopy survey device based on AD5933 chip characterized by, includes:
the oc-shaped bracket (1), one end of the oc-shaped bracket (1) is opened, and the two ends of the opening are respectively wound on the rubber rod (3);
one end of each insulating tube (2) is sleeved outside one end of the rubber rod (3), silver wires (4) penetrate through the rubber rod (3), the silver wires (4) extend out of two ends of the rubber rod (3), and the insulating tubes (2) are filled with conductive adhesive (5);
the other ends of the two rubber rods (3) are respectively connected with an AD5933 chip electrode clamp.
2. The device for measuring the impedance spectrum of the plant leaves based on the AD5933 chip as claimed in claim 1, wherein the oc-shaped bracket (1) is a spring wire, and both ends of the opening of the oc-shaped bracket (1) are respectively spirally wound on the rubber rod (3).
3. The device for measuring the impedance spectrum of the plant leaves based on the AD5933 chip as claimed in claim 1, wherein the oc-shaped bracket (1) is coated with paint on the surface of the part wound on the rubber rod (3) to insulate the part.
4. The device for measuring the impedance spectrum of the plant leaves based on the AD5933 chip as claimed in claim 1, wherein the insulation tube (2) is an insulation rubber tube, and the rubber tube is cylindrical.
5. The device for measuring the impedance spectrum of the plant leaves based on the AD5933 chip as claimed in claim 1, wherein the oc-shaped bracket (1) is made of spring wire.
6. The method for measuring the impedance spectrum of the plant leaf by using the plant leaf impedance spectrum measuring device based on the AD5933 chip as claimed in claim 1, which is characterized by comprising the following steps:
the method comprises the following steps: selecting fresh branches of plants to be detected growing in different environments with leaves, and wrapping the base parts of the branches;
step two: cleaning leaves on the fresh branches, and picking the leaves with more consistent growth vigor;
step three: the leaf is clamped between two insulating pipes (2) of the measuring device, and the voltage and the frequency are set through arduino software to measure the plant impedance spectrum.
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Cited By (1)
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