CN108508288A - Plant electric signal detection device and method - Google Patents
Plant electric signal detection device and method Download PDFInfo
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- CN108508288A CN108508288A CN201810060475.8A CN201810060475A CN108508288A CN 108508288 A CN108508288 A CN 108508288A CN 201810060475 A CN201810060475 A CN 201810060475A CN 108508288 A CN108508288 A CN 108508288A
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- G—PHYSICS
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- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
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Abstract
The present invention provides a kind of plant electric signal detection device and methods, including：Insulate vessel, first electrode, second electrode, conductive colloid, signal amplifier and data acquisition unit；The conduction colloid is attached to the upper and lower surface in the insulation vessel, one end of the first electrode is arranged in the conductive colloid of the upper surface, one end of the second electrode is arranged in the conductive colloid of the lower surface, the side that the other end of the first electrode and the other end of the second electrode both pass through the insulation vessel extends to outside the insulation vessel, and be connected with the signal amplifier, the signal amplifier is connect with the data acquisition unit.Detect plant electric signal to be measured in the case where not influencing plant normal growth to be measured, when detection uses contact type measurement, can't treat measuring plants and cause any harm.Integral material and experiment condition are very low compared to other plant electric signal measurement installation cost.
Description
Technical field
The present invention relates to Agricultural Information detection technique fields, more particularly, to plant electric signal detection device and method.
Background technology
Plant electric signal is initial reaction of the plant to environmental stimuli, after plant is stimulated, in plant
It will appear the granting sequence of action potential or action potential, that is, the potential fluctuation of plant electric signal between cell.Veggetable electricity
Signal can reflect growth and development and the nutrition condition of plant, and only carrying out quantitative analysis to plant electric signal could solve well
The mechanism of production and regulatory mechanism of plant electric signal are released, and plant electric signal is applied in interactive environmental control of greenhouse, plant
The intelligentized control method etc. of object growth and development and the diagnosis of Nutritional Status and adjusting, water-saving irrigation.
Traditional plant is extracellular or surface potential recording method is typically directly to be pierced into plant leaf blade using electrode, in this way will
Prodigious damage can be generated to plant.And since electrode needs to be pierced into plant leaf blade, the plant leaf blade after once test
It has been be damaged that, can lead to not carry out contrast experiment using identical blade, it will greatly increased experimental cost and experiment is difficult.
Invention content
It solves the above problems in order to overcome the problems referred above or at least partly, realizes that precision is high, speed is fast, at low cost, tool
There is high duplication, the detection of the live body that can be used for repeatedly the measuring plant electric signal of non-damaging formula in situ.The present invention provides one
Kind plant electrical signal detection device and method.
On the one hand, the present invention provides a kind of plant electric signal detection devices, including：Insulation vessel, first electrode, second
Electrode, conductive colloid, signal amplifier and data acquisition unit；
The conduction colloid is attached to the upper and lower surface in the insulation vessel, and one end of the first electrode is set
It sets in the conductive colloid of the upper surface, one end of the second electrode is arranged in the conductive colloid of the lower surface, institute
The other end of the other end and the second electrode of stating first electrode both pass through it is described insulation vessel side extend to it is described absolutely
Outside edge vessel, and it is connected with the signal amplifier, the signal amplifier is connect with the data acquisition unit；
It is provided in the insulation vessel and waits for measuring plants, the conductive colloid of the root for waiting for measuring plants and the lower surface
Contact, the blade for waiting for measuring plants are contacted with the conductive colloid of the upper surface.
Preferably, it is additionally provided with nutritive solution in the insulation vessel.
Preferably, the side of the insulation vessel is additionally provided with ventilation opening.
Preferably, described device further includes filter, the filter respectively with the signal amplifier and the data
Collecting unit connects.
Preferably, the first electrode and the second electrode are unpolarized electrode.
Preferably, the unpolarized electrode is specially silver electrode, silver chloride electrode, gold electrode, and platinum black electrode or glass are micro-
Electrode.
On the other hand, the plant electric signal detection method based on above-mentioned apparatus that the present invention also provides a kind of, including：
It alternately irradiates in insulation vessel and waits for by the light of the first default intensity of illumination and the light of the second default intensity of illumination
Measuring plants；
Obtain the first electric signal that the blade for waiting for measuring plants that data acquisition unit collects generates and described
Wait for the second electric signal that the root of measuring plants generates；
The difference of first electric signal and the second electric signal is waited for the detection knot of the plant electric signal of measuring plants as described in
Fruit.
Preferably, the light of the described first default intensity of illumination and the light of the second default intensity of illumination are preset wavelength
Monochromatic light or mixed wavelengths light.
Preferably, the light of the described first default intensity of illumination and the light of the second default intensity of illumination are visible light.
Preferably, it is alternately irradiated in the light by the first default intensity of illumination and the light of the second default intensity of illumination exhausted
In edge vessel wait for measuring plants before further include：
If the height for waiting for measuring plants waits for measuring plants in the insulation less than the height of the insulation vessel by described
It is cultivated in vessel, until the height for waiting for the height of measuring plants and reaching the insulation vessel.
A kind of plant electric signal detection device and method provided by the invention, by the way that conductive colloid is arranged, respectively with it is to be measured
The blade of plant and root contact, plant electric signal to be measured is detected in the case where not influencing plant normal growth to be measured, is detected
When use contact type measurement, measuring plants can't be treated and caused any harm.Integral material and experiment condition are planted compared to other
Object electric signal measurement installation cost is very low.It measures easy to operate, the fixation and the searching that carry out electrode need not be gone to wait in measuring plants
The cumbersome operation such as specific position.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram for plant electric signal detection device that one embodiment of the invention provides；
Fig. 2 is a kind of flow diagram of plant electric signal detection method provided in an embodiment of the present invention；
Fig. 3 is that a kind of curve for the plant electric signal that plant electric signal detection method obtains provided in an embodiment of the present invention shows
It is intended to.
Specific implementation mode
With reference to the accompanying drawings and examples, the specific implementation mode of the present invention is described in further detail.Implement below
Example is not limited to the scope of the present invention for illustrating the present invention.
As shown in Figure 1, one embodiment of the invention provides a kind of plant electric signal detection device, including：Insulation vessel 11,
First electrode 12, second electrode 13, conductive colloid 14, signal amplifier 15 and data acquisition unit 16；
The conduction colloid 14 is attached to the upper and lower surface in the insulation vessel 11, the first electrode 12
One end is arranged in the conductive colloid of the upper surface, and the conducting resinl in the lower surface is arranged in one end of the second electrode 13
In matter, the other end of the other end of the first electrode 12 and the second electrode 13 both passes through the side of the insulation vessel 11
It extends to outside the insulation vessel 11, and is connected with the signal amplifier 15, the signal amplifier 15 and the number
It is connected according to collecting unit 16；
It is provided in the insulation vessel 11 and waits for measuring plants 17, the root for waiting for measuring plants 17 is led with the lower surface
Electric colloid contact, the blade for waiting for measuring plants 17 are contacted with the conductive colloid of the upper surface.
Specifically, the structure of the insulation vessel used in the present invention may include vessel body and vessel cover, and insulate vessel
Interior lower surface is the bottom of vessel body, and the upper surface insulated in vessel is vessel cover.Conductive colloid is respectively attached to absolutely
The macromoleculars such as potassium chloride and potassium nitrate are added based on agar in upper and lower surface in edge vessel, the material of conductive colloid
The solution of solute and salt solute composition, solution concentration can be adjusted as needed.Conductive colloid is set to be respectively attached to insulator
The specific method of upper and lower surface in ware is：The vessel cover for the vessel that insulate is removed, by 5mmol KCl and 0.5% fine jade
Lipoprotein solution is poured on the lower surface and upper surface for so that solution is paved in insulation vessel in vessel body and in vessel cover respectively, and makes
Solution and first electrode and the good contact of second electrode simultaneously couple, and wait for solution to be cooled to solid-state and complete conductive colloid
Setting.Conductive colloid in the present invention is transparent or semitransparent state, to ensure to wait for that measuring plants can be fully irradiated by light below,
Need the light for making conductive colloid not absorb visible light wave range, i.e., absorbing wavelength is not in the light of 300-800nm, intensity of illumination it is saturating
It crosses rate to need 50% or more, the thickness range of conductive colloid is adjustable, and conductive colloid has good conductive ability, and conductance is in 100 μ
S or more.
Meanwhile to avoid insulation vessel from having an impact incident light, preferably, insulation vessel are transparent or half
Pellucidity, is made using insulating materials such as such as plastics, and wave-length coverage is not lost in 300nm- 70% or more in light transmittance
Light between 800nm.Insulate vessel inner space can according to requiring to have a greater change range, highly with the parameters such as width
Also it can adjust according to demand.Under normal circumstances, insulation vessel be semitight, but not limit and this, can also be arranged according to demand
At the form that is absolutely sealed, the present invention is not specifically limited herein.
On the basis of the above embodiments, it is additionally provided with nutritive solution in the insulation vessel, nutritive solution usually exists
KCl agar solutions are cooled to solid and pour into insulation vessel, and the height poured into can be 2-3mm.Nutritive solution may be selected 1/2
Huo Gelan (hogland) nutrient solution, to ensure to wait for the normal existence of measuring plants in insulation vessel.Allow and waits for measuring plants at this
The potential measurement that (2-5 days) for a long time are provided in the device provided is invented, the electric signal detected is stablized, compared with other devices
The electric signal stability that the device obtains is very strong.
It should be noted that the side for the vessel that insulate in the present invention can be smooth surface, can also be multiple plane structures
At, can be specifically cylindrical structure, can also be rectangular parallelepiped structure, can be selected according to the difference for waiting for measuring plants it is suitable absolutely
The shape of edge vessel is treated measuring plants and is detected.Insulation vessel are traditionally arranged to be transparent, may be set to be if needed non-
Transparent, the material for the vessel that insulate can choose the insulators such as glass, plastics.The vessel that insulate are generally semi-enclosed, and effect is real
Now wait for that the environment residing for measuring plants is controllable, semiclosed is to ensure to wait for that measuring plants can have air exchange inside insulation vessel, into
Row will not treat measuring plants physiological property when measuring for a long time has an impact, and will not lead to Plant death to be measured.Meanwhile half
Closed insulation vessel limit environmental change with the stability of the plant electric signal ensured, avoid the straight of environmental factor
Influence is connect, avoids influence of the environmental factor to conductive colloid to influence the stability of plant electric signal indirectly.Wherein, it half seals
50% or more, the ventilation aperture for the vessel that insulate can be adjusted the air humidity in insulation vessel closed according to demand.Make
For preferred embodiment, the maximum open area for the vessel that insulate is no more than a quarter of whole surface area.
In the present invention, by providing a kind of plant electric signal detection device, by the way that conductive colloid is arranged, respectively with plant to be measured
The blade of object and root contact, detect plant electric signal to be measured in the case where not influencing plant normal growth to be measured, when detection
Using contact type measurement, measuring plants can't be treated and caused any harm.Integral material and experiment condition are compared to other plant
Electric signal measurement installation cost is very low.Measure it is easy to operate, need not go carry out electrode fixation with searching it is to be measured endophytic
The cumbersome operation such as specific position.
On the basis of the above embodiments, the plant electric signal in the present invention is exported as electro physiology phenotype as a result, including
With the action potential of container contact site, make a variation current potential and local potential etc., these electric signals are to wait for that measuring plants itself produce
Raw, it can export in the form of voltage or current.To wait for that the electric signal that measuring plants itself generate is defeated in the form of voltage in the present invention
For going out, i.e. current potential, through waiting for that the first electric signal that the blade of measuring plants generates is the first current potential, through waiting for that the root of measuring plants generates
The second electric signal be the second current potential.First current potential and the second current potential are amplified by signal amplifier, and acquire list by data
Member acquisition, finally by the difference of the first current potential and the second current potential as final testing result, this is because root obtain the
For two current potentials usually as a reference potential, what is actually detected is the current potential for waiting for each leaf position of measuring plants.
The signal amplifier used in the present invention is high-impedance amplifier, specially high impedance micro-electrode amplifier, as
The input impedance of preferred embodiment, signal amplifier is not less than 1013Ohm, amplification factor are 1 times, and frequency response is：DC~
10KHz, noise are less than 200 μ V, without notable electromagnetic interference, without significantly vibrations.It should be noted that ensure to provide in the present invention
Device safety, an input end grounding of signal amplifier can be handled.Data acquisition unit in the present invention can be with
Plant electric signal is recorded by different modes such as AC or DCs.
On the basis of the above embodiments, it is needed in the present invention when the plant electric signal for treating measuring plants is detected
Stimulation is set and waits for that measuring plants generate the external environment of electric signal, that is, light and the second default illumination of the first default intensity of illumination are set
The light of intensity alternately waits for measuring plants in irradiation insulation vessel, that is to say, that can utilize two kinds and two or more different light intensity or
The combination of different wave length carries out cycle stimulation to the plant inside container, and then detection waits for measuring plants in such circumstances in real time
The plant electric signal of generation.For example, setting the first default intensity of illumination to 144 μm of ol*m-2s-1, the second default illumination is strong
Degree is set as 0 μm of ol*m-2s-1, cycle-index may be configured as 2 times and 2 times or more, every time the first default intensity of illumination in cycle
The duration of the light irradiation for the time interval and each intensity of illumination that light and the light of the second default intensity of illumination are irradiated is equal
Such as it can switch an intensity of illumination every 10 minutes according to requiring to be adjusted.Duration can be 1 minute or 10 minutes
Deng.It should be noted that the light of the first default intensity of illumination and the light of the second default intensity of illumination can be that wave-length coverage is
The visible light of 300nm-800nm can also be the result in 300nm-800nm after the optical superposition of multiple Single wavelengths.The present invention
The light of middle use can be generated by light source, and light source may include the artificial light sources such as halogen lamp, incandescent lamp.Same light is used in the present invention
The plant electric signal that photoinduction according to intensity obtains can reach 95% or more similitude, and ensure that repeatedly to measure can obtain phase
With as a result, repeatability it is very high.
On the basis of the above embodiments, the light of the described first default intensity of illumination and the second default intensity of illumination
Light is the monochromatic light or mixed wavelengths light of preset wavelength.It is the visible of 300nm-800nm that mixed wavelengths light, which refers to wave-length coverage,
The value range of light, preset wavelength is also 300nm-800nm, and it is 500nm such as to choose preset wavelength, in 144 μm of ol*m-2s-1With 0
μmol*m-2s-1Between switch over light intensity, the time interval of switching is 10 minutes.
On the basis of the above embodiments, described device further includes filter 18, the filter 18 respectively with the letter
Number amplifier 15 and the data acquisition unit 16 connect.
Specifically, since there may be noises in the electric signal for waiting for measuring plants that is passed out through first electrode and second electrode
The detection of practical plant electric signal is influenced, so filter is accessed after signal amplifier, the noise in filtering electric signal.As
Preferred embodiment, the present invention use low-pass filter, and cutoff frequency is adjustable between 10Hz-10KHz, i.e. the passband of low-pass filter
Ranging from 0 to cutoff frequency.
The sample frequency for the data acquisition unit that the present invention uses is more than 200Hz, and common-mode rejection ratio is more than 100dB, noise
Less than ± 3 microvolts (μ V), it is 5 μ V to 1V to allow the voltage range of input.
On the basis of the above embodiments, the conductive colloid that the present invention uses is based on low concentration potassium chloride and agar, tool
There is a higher conductivity, and with certain viscosity, there is no injury effect to plant leaf blade and root, and waits for measuring plants and the first electricity
Pole, second electrode all have good coupling.It is noted that conductive colloid cannot form electricity time with plant to be measured when in use
Road, it is not possible to same conduction colloid not only connected to the blade for waiting for measuring plants but also connected the root for waiting for measuring plants.
On the basis of the above embodiments, the side of the insulation vessel is additionally provided with ventilation opening, and the ventilation opening is opened
Open area is less than a quarter of the lateralarea of the insulation vessel.
On the basis of the above embodiments, the first electrode and the second electrode are unpolarized electrode.The non-pole
Polarizing electrode is specially silver chloride electrode, gold electrode, platinum black electrode or glass microelectrode.
On the basis of the above embodiments, wait for that measuring plants are usually higher plant, such as bryophyte, fern in the present invention
Class Plants and Seeds plant, but it is not limited to this.Wait for that the height of measuring plants is equal to or slightly above the height of insulation vessel, it is to be measured
The root of plant is coupled with the conductive colloid on the lower surface in insulation vessel, culture solution with good, waits for the leaf of measuring plants
Piece good is coupled with the conductive colloid on the upper surface of insulation vessel.When wait for the height of measuring plants be not enough to contact insulation vessel
Upper surface on conductive colloid when, then can will after measuring plants insulation vessel in carry out a period of time culture after make it is to be measured
It is detected again after the blade of plant and the conductive colloid good coupling on the upper surface of insulation vessel.Doing such setting is mainly
The normal growth for influencing to wait for measuring plants is avoided, belongs to non-nocuity detection, allows to wait for that measuring plants are led with non-irritating naturally
Electric colloid is bonded, and need not be treated measuring plants and be carried out strong compressing, ensure the stabilization for the plant electric signal that detection obtains
Property.
On the basis of the above embodiments, wait for that the blade contacted with the conductive colloid of upper surface on measuring plants generates average electricity
Position V1, wait for that the root of measuring plants generates average potential V2, record V1With V2Difference, as this wait for measuring plants receive occulting light friendship
For the plant electric signal generated after irradiation.Wherein, V1It depends primarily on and waits for micro-scaled range around the blade contact face of measuring plants
And the current potential average value that the plant cell activity of depth bounds generates, V2Then depend on waiting for the root of the root contact surface of measuring plants
Area's cell potential average value.The difference V recorded1-V2The potential change for depending primarily on two contact surface periphery cells, with it
His cell of position is unrelated.
On the basis of the above embodiments, described device further includes：Respectively corresponding with first electrode and second electrode
One electrode connector and second electrode connector, the both ends of first electrode connector respectively with first electrode and signal amplifier
Grade is practiced in one interface connection, the both ends of second electrode connector with another interface of second electrode and signal amplifier respectively.The
One electrode connector and second electrode connector can also be replaced with crocodile clip, carried out electrode and picked out a little company with signal amplifier
It connects.
Specifically, the building method of plant electric signal detection device provided in the present invention is：By transparent organic glass
Or the hole that the closing vessel middle part position with cover of plastic production is about 1-2mm using one diameter of tool open is used for sample
Product are ventilated, i.e., ventilation opening are arranged on insulation vessel.One is opened respectively directly in the side of vessel cover with insulation vessel bottom
About the hole of 1mm is for being placed in first electrode and second electrode.Diameter is less than to KCl solution of the filamentary silver in 100mmol of 1mm
Middle plating is the non-polarised first electrodes of Ag/AgCl and second electrode, and the length of two electrodes can be depending on container dimensional, will
Wherein one end of two electrodes is respectively welded conducting wire and is used for drawing plant electric signal.It is being placed in and fixed electroplated perfect two
Cavity is sealed using hot melt adhesive is slight after a electrode.Then vessel cover is removed, uses 0.5% fine jade of 5mmol KCl
Lipoprotein solution be poured in vessel body in vessel cover, make agar solution and first electrode and second electrode carry out it is good couple,
And the 1/2hogland nutrient solutions that height is about 2-3mm are poured into the bottom of vessel body after waiting for agar to be cooled to solid-state.
As shown in Fig. 2, on the basis of the above embodiments, the present invention also provides one kind realizing plant based on above-mentioned apparatus
The method of electrical signal detection, including：
S1 is alternately irradiated by the light of the first default intensity of illumination and the light of the second default intensity of illumination in insulation vessel
Wait for measuring plants；
S2 obtains the first electric signal that the blade for waiting for measuring plants that data acquisition unit collects generates, and
The second electric signal that the root for waiting for measuring plants generates；
The difference of first electric signal and the second electric signal is waited for the detection of the plant electric signal of measuring plants by S3 as described in
As a result.
On the basis of the above embodiments, the light of the described first default intensity of illumination and the second default intensity of illumination
Light is the monochromatic light or mixed wavelengths light of preset wavelength.
On the basis of the above embodiments, the light of the described first default intensity of illumination and the second default intensity of illumination
Light is visible light.
On the basis of the above embodiments, in the light by the first default intensity of illumination and the second default intensity of illumination
Light alternately irradiation insulation vessel in wait for measuring plants before further include：
If the height for waiting for measuring plants waits for measuring plants in the insulation less than the height of the insulation vessel by described
It is cultivated in vessel, until the height for waiting for the height of measuring plants and reaching the insulation vessel.
Specifically, the operating process in the present embodiment the step of each method and in above-mentioned apparatus embodiment and explanation one are a pair of
It answers, details are not described herein for the present embodiment.
The placement location of measuring plants is waited in the present invention as shown in Figure 1, waiting for that the root of measuring plants is immersed in hogland nutrient solutions
In, while waiting for that the blade position of measuring plants has good couple with the conductive colloid of upper surface.By first electrode and the second electricity
Pole is connected to two incoming ends of signal amplifier, and waits for measuring plants using the alternate light irradiation of light and shade, so that it may be waited for being recorded
The plant electric signal that measuring plants are stablized, as shown in Figure 3.A curve indicates do not have in plant electric signal detection device above in Fig. 3
There is a curve of the plant electric signal obtained by data acquisition unit when high-pass filter, a curve indicates plant below in Fig. 3
There are the veggetable electricities obtained by data acquisition unit when the high-pass filter that cutoff frequency is 0.16Hz in electrical signal detection device
The curve of signal.The position of corresponding two curves up and down of white box in Fig. 3 is indicated by 144 μm of ol*m-2s-1Light irradiation
The position of the value of the plant electric signal obtained when measuring plants, corresponding two curves up and down of black box in Fig. 3 indicates
By 0 μm of ol*m-2s-1The value of plant electric signal that is obtained when measuring plants of light irradiation.Abscissa in Fig. 3 is the time, is indulged
Coordinate is mV.It can also be seen that occulting light alternately irradiates obtained plant electric signal each time from Fig. 3 and all have very high phase
Like property.
As can be seen from the above embodiments, by using semienclosed environment control method, non-nocuity in the present invention
Detection method, non-polarized microelectrode draws signal, finally measured plant leaf blade and root zone high duplication under light stimulation
Potential difference.
Finally, method of the invention is only preferable embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in the protection of the present invention
Within the scope of.
Claims (10)
1. a kind of plant electric signal detection device, which is characterized in that including：Insulate vessel, first electrode, second electrode, conduction
Colloid, signal amplifier and data acquisition unit；
The conduction colloid is attached to the upper and lower surface in the insulation vessel, and one end setting of the first electrode exists
In the conductive colloid of the upper surface, one end of the second electrode is arranged in the conductive colloid of the lower surface, and described
The side that the other end of one electrode and the other end of the second electrode both pass through the insulation vessel extends to the insulator
Outside ware, and it is connected with the signal amplifier, the signal amplifier is connect with the data acquisition unit；
It is provided in the insulation vessel and waits for measuring plants, the conductive colloid of the root for waiting for measuring plants and the lower surface connects
It touches, the blade for waiting for measuring plants is contacted with the conductive colloid of the upper surface.
2. the apparatus according to claim 1, which is characterized in that be additionally provided with nutritive solution in the insulation vessel.
3. the apparatus according to claim 1, which is characterized in that the side of the insulation vessel is additionally provided with ventilation opening.
4. device according to any one of claim 1-3, which is characterized in that further include filter, the filter point
It is not connect with the signal amplifier and the data acquisition unit.
5. device according to any one of claim 1-3, which is characterized in that the first electrode and the second electrode
For unpolarized electrode.
6. device according to claim 5, which is characterized in that the unpolarized electrode is specially silver chloride electrode, gold electricity
Pole, platinum black electrode or glass microelectrode.
7. a kind of plant electric signal detection method based on such as claim 1-6 described devices, which is characterized in that including：
Pass through the light of the first default intensity of illumination and the light plant to be measured that alternately irradiation is insulated in vessel of the second default intensity of illumination
Object；
Obtain the first electric signal that the blade for waiting for measuring plants that data acquisition unit collects generates and described to be measured
The second electric signal that the root of plant generates；
The difference of first electric signal and the second electric signal is waited for the testing result of the plant electric signal of measuring plants as described in.
8. the method according to the description of claim 7 is characterized in that the light of the first default intensity of illumination and described second is in advance
If the light of intensity of illumination is the monochromatic light or mixed wavelengths light of preset wavelength.
9. the method according to the description of claim 7 is characterized in that the light of the first default intensity of illumination and described second is in advance
If the light of intensity of illumination is visible light.
10. according to the method described in any one of claim 7-9, which is characterized in that described strong by the first default illumination
The light of degree and the light of the second default intensity of illumination alternately in irradiation insulation vessel wait for measuring plants before further include：
If the height for waiting for measuring plants waits for measuring plants in the insulation vessel less than the height of the insulation vessel by described
It is inside cultivated, until the height for waiting for the height of measuring plants and reaching the insulation vessel.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109959832A (en) * | 2019-04-25 | 2019-07-02 | 福建农林大学 | A kind of plant electric signal acquisition platform and method |
| CN112305313A (en) * | 2020-10-29 | 2021-02-02 | 北京林业大学 | Plant leaf impedance spectrum measuring device and method based on AD5933 chip |
| CN113176301A (en) * | 2021-04-30 | 2021-07-27 | 中国科学院地球化学研究所 | Method for detecting plant health activity based on electrophysiological rhythm |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202006005185U1 (en) * | 2006-03-31 | 2006-07-06 | Chen, Lu, Dr. | Water shortage warning device for indoor plant, has control panel connected with electrodes, and connector to output electrical signals, where humidity of soil is collected by stream flow to provide information about water shortage |
| CN101021518A (en) * | 2006-11-01 | 2007-08-22 | 中国农业大学 | Method for detecting plant electric signal in green house and special equipment thereof |
| CN101666767A (en) * | 2009-10-10 | 2010-03-10 | 北京航空航天大学 | Multichannel plant hydropenia monitoring system based on impedance measurement |
| CN201503407U (en) * | 2009-10-10 | 2010-06-09 | 张晗 | Non-invasive plant hydropenia monitoring system based on measurement of impedance |
| CN204008535U (en) * | 2014-08-18 | 2014-12-10 | 江苏大学 | The test macro of the critical freeze injury temperature of one Plants |
| CN204102010U (en) * | 2014-09-25 | 2015-01-14 | 天津职业技术师范大学 | Plant electric signal detects contrast experiment's device |
-
2018
- 2018-01-22 CN CN201810060475.8A patent/CN108508288B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202006005185U1 (en) * | 2006-03-31 | 2006-07-06 | Chen, Lu, Dr. | Water shortage warning device for indoor plant, has control panel connected with electrodes, and connector to output electrical signals, where humidity of soil is collected by stream flow to provide information about water shortage |
| CN101021518A (en) * | 2006-11-01 | 2007-08-22 | 中国农业大学 | Method for detecting plant electric signal in green house and special equipment thereof |
| CN101666767A (en) * | 2009-10-10 | 2010-03-10 | 北京航空航天大学 | Multichannel plant hydropenia monitoring system based on impedance measurement |
| CN201503407U (en) * | 2009-10-10 | 2010-06-09 | 张晗 | Non-invasive plant hydropenia monitoring system based on measurement of impedance |
| CN204008535U (en) * | 2014-08-18 | 2014-12-10 | 江苏大学 | The test macro of the critical freeze injury temperature of one Plants |
| CN204102010U (en) * | 2014-09-25 | 2015-01-14 | 天津职业技术师范大学 | Plant electric signal detects contrast experiment's device |
Non-Patent Citations (3)
| Title |
|---|
| 孟晓丽 等: "三种植物叶片电信号的特征分析", 《西安理工大学学报》 * |
| 汪懋华 等: "《现代精细农业理论与实践》", 31 October 2012 * |
| 赵东杰 等: "基于模型及实验的植物电信号胞外测量关键问题研究", 《中国科学：信息科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109959832A (en) * | 2019-04-25 | 2019-07-02 | 福建农林大学 | A kind of plant electric signal acquisition platform and method |
| CN109959832B (en) * | 2019-04-25 | 2024-02-23 | 福建农林大学 | Plant electric signal acquisition platform and method |
| CN112305313A (en) * | 2020-10-29 | 2021-02-02 | 北京林业大学 | Plant leaf impedance spectrum measuring device and method based on AD5933 chip |
| CN113176301A (en) * | 2021-04-30 | 2021-07-27 | 中国科学院地球化学研究所 | Method for detecting plant health activity based on electrophysiological rhythm |
| CN113176301B (en) * | 2021-04-30 | 2024-04-30 | 中国科学院地球化学研究所 | Method for detecting plant health vigor based on electrophysiological rhythm |
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