CN108508288B - Plant electric signal detection device and method - Google Patents
Plant electric signal detection device and method Download PDFInfo
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- CN108508288B CN108508288B CN201810060475.8A CN201810060475A CN108508288B CN 108508288 B CN108508288 B CN 108508288B CN 201810060475 A CN201810060475 A CN 201810060475A CN 108508288 B CN108508288 B CN 108508288B
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Abstract
The invention provides a plant electric signal detection device and a plant electric signal detection method, wherein the plant electric signal detection device comprises the following steps: the device comprises an insulating vessel, a first electrode, a second electrode, conductive colloid, a signal amplifier and a data acquisition unit; the conductive colloid is attached to the upper surface and the lower surface in the insulating vessel, one end of the first electrode is arranged in the conductive colloid on the upper surface, one end of the second electrode is arranged in the conductive colloid on the lower surface, the other end of the first electrode and the other end of the second electrode both penetrate through the side surface of the insulating vessel and extend out of the insulating vessel, and are both connected with the signal amplifier, and the signal amplifier is connected with the data acquisition unit. The electrical signal of the plant to be detected is detected under the condition that the normal growth of the plant to be detected is not influenced, and the plant to be detected is not damaged by using contact measurement during detection. Compared with other plant electric signal measuring devices, the cost of the whole material and the experimental condition is very low.
Description
Technical Field
The invention relates to the technical field of agricultural information detection, in particular to a plant electric signal detection device and method.
Background
The plant electric signal is an initial response of a plant body to an external stimulus, and after the plant body is stimulated, an action potential or an action potential distribution sequence, that is, a potential fluctuation of the plant electric signal, occurs between cells in the plant body. The plant electric signals can reflect the growth, development and nutrition conditions of plants, the generation mechanism and the regulation mechanism of the plant electric signals can be well explained only by carrying out quantitative analysis on the plant electric signals, and the plant electric signals are applied to the aspects of interactive greenhouse environment regulation, diagnosis and regulation of the growth, development and nutrition health conditions of the plants, intelligent control of water-saving irrigation and the like.
The traditional plant extracellular or surface potential recording method usually utilizes an electrode to directly penetrate into the plant leaf, which causes great damage to the plant. And because the electrode need pierce the plant leaf, carry out a experiment after the plant leaf damaged, can lead to utilizing the same leaf to carry out the contrast experiment, will greatly increased experiment cost and experiment difficulty.
Disclosure of Invention
In order to overcome the problems or at least partially solve the problems, the in-situ non-damage type plant electric signal detection device has the advantages of high precision, high speed, low cost and high repeatability, and can be used for detecting in-situ non-damage plant electric signals of a living body for multiple times of measurement. The invention provides a plant electric signal detection device and method.
In one aspect, the present invention provides a plant electrical signal detection device, including: the device comprises an insulating vessel, a first electrode, a second electrode, conductive colloid, a signal amplifier and a data acquisition unit;
the conductive colloid is attached to the upper surface and the lower surface in the insulating vessel, one end of the first electrode is arranged in the conductive colloid on the upper surface, one end of the second electrode is arranged in the conductive colloid on the lower surface, the other end of the first electrode and the other end of the second electrode both penetrate through the side surface of the insulating vessel and extend out of the insulating vessel, and are both connected with the signal amplifier, and the signal amplifier is connected with the data acquisition unit;
the insulation container is internally provided with a plant to be detected, the root of the plant to be detected is in contact with the conductive colloid on the lower surface, and the blade of the plant to be detected is in contact with the conductive colloid on the upper surface.
Preferably, a nutrient solution is also provided in the insulating vessel.
Preferably, the side surface of the insulation vessel is further provided with a vent.
Preferably, the device further comprises a filter, and the filter is respectively connected with the signal amplifier and the data acquisition unit.
Preferably, the first electrode and the second electrode are non-polarized electrodes.
Preferably, the non-polarized electrode is specifically a silver electrode, a silver chloride electrode, a gold electrode, a platinum black electrode or a glass microelectrode.
On the other hand, the invention also provides a plant electric signal detection method based on the device, which comprises the following steps:
alternately irradiating the plant to be detected in the insulating vessel by using light with the first preset illumination intensity and light with the second preset illumination intensity;
acquiring a first electric signal generated by the leaves of the plant to be detected and acquired by a data acquisition unit and a second electric signal generated by the roots of the plant to be detected;
and taking the difference between the first electric signal and the second electric signal as the detection result of the plant electric signal of the plant to be detected.
Preferably, the light with the first preset illumination intensity and the light with the second preset illumination intensity are both monochromatic light with a preset wavelength or light with a mixed wavelength.
Preferably, the light with the first preset illumination intensity and the light with the second preset illumination intensity are both visible light.
Preferably, before the plant to be tested in the insulating vessel is alternatively irradiated by the light with the first preset illumination intensity and the light with the second preset illumination intensity, the method further comprises:
and if the height of the plant to be detected is lower than that of the insulating vessel, culturing the plant to be detected in the insulating vessel until the height of the plant to be detected reaches the height of the insulating vessel.
According to the plant electric signal detection device and method provided by the invention, the conductive colloid is arranged and is respectively contacted with the leaves and the roots of the plants to be detected, the plant electric signals to be detected are detected under the condition that the normal growth of the plants to be detected is not influenced, and the plants to be detected are not damaged by using contact measurement during detection. Compared with other plant electric signal measuring devices, the cost of the whole material and the experimental condition is very low. The measurement operation is simple, and complicated operations such as fixing the electrode and searching for a specific position in the plant to be measured are not needed.
Drawings
Fig. 1 is a schematic structural diagram of a plant electrical signal detection device according to an embodiment of the present invention;
FIG. 2 is a schematic flow chart of a plant electrical signal detection method according to an embodiment of the present invention;
fig. 3 is a schematic graph of a plant electrical signal obtained by the plant electrical signal detection method according to the embodiment of the present invention.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
As shown in fig. 1, an embodiment of the present invention provides a plant electrical signal detection apparatus, including: the device comprises an insulating vessel 11, a first electrode 12, a second electrode 13, conductive colloid 14, a signal amplifier 15 and a data acquisition unit 16;
the conductive colloid 14 is attached to the upper surface and the lower surface in the insulating vessel 11, one end of the first electrode 12 is arranged in the conductive colloid on the upper surface, one end of the second electrode 13 is arranged in the conductive colloid on the lower surface, the other ends of the first electrode 12 and the second electrode 13 both penetrate through the side surface of the insulating vessel 11 and extend out of the insulating vessel 11, and are both connected with the signal amplifier 15, and the signal amplifier 15 is connected with the data acquisition unit 16;
a plant 17 to be tested is arranged in the insulating vessel 11, the root of the plant 17 to be tested is in contact with the conductive colloid on the lower surface, and the blade of the plant 17 to be tested is in contact with the conductive colloid on the upper surface.
Specifically, the insulating vessel adopted in the invention can comprise a vessel main body and a vessel cover, wherein the lower surface in the insulating vessel is the bottom of the vessel main body, and the upper surface in the insulating vessel is the vessel cover. The conductive colloid is attached to the upper surface and the lower surface in the insulating vessel respectively, the conductive colloid is mainly made of agar, and a solution composed of macromolecular solutes such as potassium chloride and potassium nitrate and salt solutes is added, so that the concentration of the solution can be adjusted as required. The specific method for respectively attaching the conductive colloid to the upper surface and the lower surface in the insulating vessel comprises the following steps: and taking down the container cover of the insulating container, pouring 5mmol KCl and 0.5% agar solution into the container body and the container cover respectively, enabling the solution to be spread on the lower surface and the upper surface in the insulating container, enabling the solution to be in good contact and coupled with the first electrode and the second electrode, and waiting until the solution is cooled to be solid, thus completing the setting of the conductive colloid. The conductive colloid is in a transparent or semitransparent state, so that in order to ensure that a plant to be detected can be fully irradiated by light, the conductive colloid needs to not absorb light in a visible light waveband, namely light with the wavelength of 300-800nm, the transmittance of the illumination intensity needs to be over 50 percent, the thickness range of the conductive colloid is adjustable, the conductive colloid has good conductive capability, and the electrical conductivity is over 100 microseconds.
Meanwhile, in order to avoid the influence of the insulating vessel on the incident light, as a preferred scheme, the insulating vessel is in a transparent or semitransparent state, is made of insulating materials such as plastics and the like, has the light transmittance of 70 percent or more, and does not lose the light with the wavelength range of 300nm-800 nm. The internal space of the insulating vessel can have a large variation range according to requirements, and parameters such as height, width and the like can be adjusted according to requirements. Generally, the insulating vessel is a semi-sealed vessel, but the invention is not limited thereto, and may be provided in an absolute sealed form according to the requirement, and the invention is not limited thereto.
On the basis of the above embodiment, a nutrient solution is also arranged in the insulating vessel, and the nutrient solution is usually cooled in a KCl-agar solution until the solid is poured into the insulating vessel, wherein the pouring height can be 2-3 mm. The nutrient solution can be 1/2Hogland (hogland) nutrient solution to ensure the normal survival of the plant to be detected in the insulated container. The plant to be detected can carry out potential measurement for a long time (2-5 days) in the device provided by the invention, the detected electric signal is stable, and the stability of the electric signal obtained by the device is very strong compared with other devices.
It should be noted that the side surface of the insulating container in the invention may be a smooth curved surface, or may be formed by a plurality of planes, specifically, it may be a cylindrical structure, or may be a rectangular structure, and the shape of the insulating container may be selected according to the difference of the plants to be detected to detect the plants to be detected. The insulating vessel is generally transparent, and may be non-transparent if necessary, and the material of the insulating vessel may be selected from glass, plastic and other insulators. The insulating vessel is generally semi-closed, and the effect is to realize that the environment that the plant that awaits measuring is located is controllable, and semi-closed is to guarantee that the plant that awaits measuring can have air exchange in insulating vessel, can not exert an influence to the physiological characteristic of the plant that awaits measuring when carrying out the long-time measurement, also can not lead to the plant death that awaits measuring. Meanwhile, the semi-closed insulating vessel limits the environmental change so as to ensure the stability of the obtained plant electric signal, avoid the direct influence of environmental factors and avoid the influence of the environmental factors on the conductive colloid so as to indirectly influence the stability of the plant electric signal. Wherein, the air humidity in the semi-closed insulating ware is more than 50%, and the ventilation aperture of insulating ware can be adjusted as required. Preferably, the maximum open area of the insulating vessel is no more than one quarter of the total surface area.
According to the plant electric signal detection device, the conductive colloid is arranged and is respectively contacted with the leaves and the roots of the plants to be detected, so that the plant electric signals to be detected are detected under the condition that the normal growth of the plants to be detected is not influenced, and the plants to be detected are not damaged by using contact measurement during detection. Compared with other plant electric signal measuring devices, the cost of the whole material and the experimental condition is very low. The measurement operation is simple, and complicated operations such as fixing the electrode and searching for a specific position in the plant to be measured are not needed.
In addition to the above embodiments, the plant electric signals in the present invention include the action potential, the variation potential, the local potential, and the like of the contact part with the container as the electrophysiological phenotype output result, and these electric signals are generated by the plant to be tested itself and can be output in the form of voltage or current. In the present invention, the electrical signal generated by the plant to be tested is output in the form of voltage, that is, the electrical potential, the first electrical signal generated by the leaf of the plant to be tested is the first electrical potential, and the second electrical signal generated by the root of the plant to be tested is the second electrical potential. The first potential and the second potential are amplified by the signal amplifier and collected by the data acquisition unit, and finally the difference value of the first potential and the second potential is used as a final detection result, because the second potential obtained from the root is usually used as a reference potential, and actually the potential of each leaf position of the plant to be detected is detected.
The signal amplifier adopted in the invention is a high-impedance amplifier, in particular a high-impedance microelectrode amplifier, and as a preferred scheme, the input impedance of the signal amplifier is not lower than 1013Ohm, magnification 1, frequency response: DC-10 KHz, noise lower than 200 μ V, no significant electromagnetic interference and no significant vibration. It should be noted that, in order to ensure the safety of the device provided in the present invention, one input terminal of the signal amplifier may be grounded. The data acquisition unit in the invention can record the plant electric signal in different modes such as alternating current or direct current.
On the basis of the above embodiment, in the invention, when detecting the plant electrical signal of the plant to be detected, an external environment for stimulating the plant to be detected to generate the electrical signal needs to be set, that is, the light with the first preset illumination intensity and the light with the second preset illumination intensity are set to alternately irradiate the plant to be detected in the insulating container, that is, the combination of two or more different light intensities or different wavelengths can be utilized to circularly stimulate the plant in the container, and then the plant electrical signal generated by the plant to be detected in the environment is detected in real time. For example, the first preset illumination intensity is set to 144 μmol m-2s-1Setting the second preset illumination intensity to 0 [ mu ] mol m-2s-1The number of cycles can be set to 2 or more than 2, and the time interval between the irradiation of the first preset illumination intensity light and the second preset illumination intensity light and the duration of the irradiation of each illumination intensity light in each cycle can be adjusted according to the requirements, for example, every 10 minutesThe clock switches the illumination intensity once. The duration may be 1 minute or 10 minutes, etc. It should be noted that both the light with the first preset illumination intensity and the light with the second preset illumination intensity may be visible light with a wavelength range of 300nm to 800nm, or may be a result of overlapping a plurality of lights with single wavelength within 300nm to 800 nm. The light used in the present invention may be generated by a light source, which may include a halogen lamp, an incandescent lamp, and the like, artificial light sources. The plant electric signals obtained by light induction with the same illumination intensity can reach the similarity of more than 95 percent, so that the same result can be obtained by multiple measurements, and the repeatability is very high.
On the basis of the above embodiment, the light with the first preset illumination intensity and the light with the second preset illumination intensity are both monochromatic light with a preset wavelength or mixed-wavelength light. The mixed wavelength light refers to visible light with wavelength range of 300nm-800nm, and the preset wavelength range is 300nm-800nm, such as 500nm at 144 μmol × m-2s-1With 0. mu. mol x m-2s-1The light intensity is switched, and the time interval of the switching is 10 minutes.
On the basis of the above embodiment, the apparatus further comprises a filter 18, and the filter 18 is respectively connected with the signal amplifier 15 and the data acquisition unit 16.
Specifically, because noise may exist in the electric signals of the plant to be detected transmitted by the first electrode and the second electrode to influence the detection of the actual plant electric signals, a filter is connected after the signal amplifier to filter the noise in the electric signals. Preferably, the low-pass filter is adopted, the cut-off frequency is adjustable between 10Hz and 10KHz, namely the pass-band range of the low-pass filter is 0 to the cut-off frequency.
The sampling frequency of the data acquisition unit adopted by the invention is more than 200Hz, the common mode rejection ratio is more than 100dB, the noise is lower than +/-3 microvolts (MuV), and the allowable input voltage range is 5 MuV to 1V.
On the basis of the embodiment, the conductive colloid adopted by the invention mainly comprises low-concentration potassium chloride and agar, has higher conductivity and certain viscosity, has no harm to the leaves and roots of the plants, and has good coupling with the plants to be detected, the first electrode and the second electrode. It should be noted that when in use, the conductive colloid cannot form an electric loop with the plant to be detected, and the same conductive colloid cannot be connected with both the leaf and the root of the plant to be detected.
On the basis of the embodiment, the side surface of the insulating vessel is also provided with a vent, and the opening area of the vent is smaller than one fourth of the side surface area of the insulating vessel.
On the basis of the above embodiment, the first electrode and the second electrode are non-polarized electrodes. The non-polarized electrode is specifically a silver chloride electrode, a gold electrode, a platinum black electrode or a glass microelectrode.
On the basis of the above embodiments, the plant to be tested in the present invention is generally higher plants, such as bryophyte, fern and seed plant, but not limited thereto. The height of the plant to be detected is equal to or slightly higher than that of the insulating vessel, the root of the plant to be detected is well coupled with the conductive colloid and the culture solution on the lower surface in the insulating vessel, and the leaf of the plant to be detected is well coupled with the conductive colloid on the upper surface of the insulating vessel. When the height of the plant to be detected is not enough to contact the conductive colloid on the upper surface of the insulating vessel, the plant to be detected can be cultured in the insulating vessel for a period of time, and then the leaf of the plant to be detected is well coupled with the conductive colloid on the upper surface of the insulating vessel for detection. The device is mainly used for avoiding influencing the normal growth of the plant to be detected, belongs to non-destructive detection, enables the plant to be detected to be naturally attached to non-irritant conductive colloid, does not need to strongly press the plant to be detected, and ensures the stability of the plant electric signal obtained by detection.
On the basis of the above embodiment, the average potential V is generated on the leaf of the plant to be tested, which is in contact with the conductive colloid on the upper surface1The root of the plant to be tested generates an average potential V2Record V1And V2The difference value is the plant electric signal generated after the plant to be detected receives the alternating illumination of the bright light and the dark light. Wherein, V1Mainly depending on the measurement to be madeMean value of the potentials, V, generated by the action of plant cells in the micrometer range and in the depth range around the leaf contact surface of a plant2It depends on the average value of the cell potential of the root zone of the root contact surface of the plant to be tested. Recorded difference V1-V2Mainly depends on the potential change of cells around the two contact surfaces, and is independent of cells at other positions.
On the basis of the above embodiment, the apparatus further includes: and the two ends of the second electrode connector are respectively connected with the second electrode and the other interface of the signal amplifier. The first electrode connector and the second electrode connector can also be replaced by crocodile clips, and the connection of the electrode outgoing point and the signal amplifier is carried out.
Specifically, the construction method of the plant electric signal detection device provided by the invention comprises the following steps: a hole with the diameter of about 1-2mm is opened at the middle part of a closed vessel made of transparent organic glass or plastic and provided with a cover by using a tool for ventilating a sample, namely, a vent is arranged on an insulating vessel. A hole with the diameter of about 1mm is directly formed in the side surfaces of the vessel cover and the bottom of the insulating vessel respectively for placing the first electrode and the second electrode. Electroplating silver wires with the diameter of less than 1mm in a KCl solution of 100mmol into Ag/AgCl non-polarized first electrodes and second electrodes, wherein the lengths of the two electrodes can be determined according to the size of a container, and welding wires at one ends of the two electrodes respectively for leading out plant electric signals. After the two electrodes which are completely electroplated are placed and fixed, the hollow hole is slightly sealed by using hot melt adhesive. Then the vessel cover is taken down, 0.5% agar solution of 5mmol KCl is poured into the vessel body and the vessel cover, the agar solution is well coupled with the first electrode and the second electrode, and 1/2hogland nutrient solution with the height of about 2-3mm is poured into the bottom of the vessel body after the agar is cooled to be solid.
As shown in fig. 2, on the basis of the above embodiment, the present invention further provides a method for detecting plant electrical signals based on the above apparatus, including:
s1, alternately irradiating the plant to be detected in the insulating container by the light with the first preset illumination intensity and the light with the second preset illumination intensity;
s2, acquiring a first electric signal generated by the leaves of the plant to be detected and acquired by the data acquisition unit and a second electric signal generated by the roots of the plant to be detected;
and S3, taking the difference between the first electric signal and the second electric signal as the detection result of the plant electric signal of the plant to be detected.
On the basis of the above embodiment, the light with the first preset illumination intensity and the light with the second preset illumination intensity are both monochromatic light with a preset wavelength or mixed-wavelength light.
On the basis of the above embodiment, the light of the first preset illumination intensity and the light of the second preset illumination intensity are both visible light.
On the basis of the above embodiment, before the plant to be tested in the insulating container is alternatively irradiated by the light with the first preset illumination intensity and the light with the second preset illumination intensity, the method further includes:
and if the height of the plant to be detected is lower than that of the insulating vessel, culturing the plant to be detected in the insulating vessel until the height of the plant to be detected reaches the height of the insulating vessel.
Specifically, the steps of the methods in this embodiment correspond to the operation processes and explanations in the above device embodiments one to one, and the details of this embodiment are not repeated herein.
The placement position of the plant to be detected is shown in figure 1, the root of the plant to be detected is immersed in the hogland nutrient solution, and meanwhile, the leaf part of the plant to be detected is well coupled with the conductive colloid on the upper surface. The first electrode and the second electrode are connected to two access ends of the signal amplifier, and the plant to be detected is irradiated by light and dark which are alternated, so that a stable plant electric signal of the plant to be detected can be recorded, as shown in fig. 3. FIG. 3 is a graph showing the plant electric signal obtained by the data acquisition unit when the high-pass filter is not provided in the plant electric signal detection apparatusThe lower curve in 3 represents the curve of the plant electric signal obtained by the data acquisition unit when a high-pass filter with the cutoff frequency of 0.16Hz exists in the plant electric signal detection device. The positions of the upper and lower two curves corresponding to the white boxes in FIG. 3 are indicated by 144. mu. mol m-2s-1The position of the upper and lower two curves corresponding to the black square in FIG. 3 is represented by 0 μmol × m-2s-1The value of the plant electrical signal obtained when the plant to be tested is irradiated by the light. The abscissa in fig. 3 is time and the ordinate is mV. As can be seen from FIG. 3, the electrical signals of the plants obtained by each alternating illumination of the bright light and the dark light have high similarity.
According to the embodiments, the semi-closed environment control method, the non-destructive detection method and the non-polarized microelectrode lead out signals, and finally the potential difference value with high repeatability between the plant leaf and the root zone under the illumination stimulation is measured.
Finally, the method of the present invention is only a preferred embodiment and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A plant electrical signal detection device, comprising: the device comprises an insulating vessel, a first electrode, a second electrode, conductive colloid, a signal amplifier and a data acquisition unit;
the conductive colloid is attached to the upper surface and the lower surface in the insulating vessel, one end of the first electrode is arranged in the conductive colloid on the upper surface, one end of the second electrode is arranged in the conductive colloid on the lower surface, the other end of the first electrode and the other end of the second electrode both penetrate through the side surface of the insulating vessel and extend out of the insulating vessel, and are both connected with the signal amplifier, and the signal amplifier is connected with the data acquisition unit;
a plant to be detected is arranged in the insulating vessel, the root of the plant to be detected is in contact with the conductive colloid on the lower surface, and the blade of the plant to be detected is in contact with the conductive colloid on the upper surface; the insulating vessel does not lose light between 300nm and 800 nm;
the specific method for respectively attaching the conductive colloid to the upper surface and the lower surface in the insulating vessel comprises the following steps: taking down a utensil cover of the insulating utensil, pouring 5mmol KCl and 0.5% agar solution into the utensil main body and the utensil cover respectively, enabling the solution to be paved on the lower surface and the upper surface in the insulating utensil, enabling the solution to be in good contact and coupled with the first electrode and the second electrode, and finishing the setting of the conductive colloid after the solution is cooled to a solid state; the conductive gel does not absorb light in the visible band.
2. The apparatus of claim 1, wherein a nutrient solution is further disposed within the insulating vessel.
3. The apparatus of claim 1, wherein the side of the insulating vessel is further provided with a vent.
4. The apparatus of any one of claims 1-3, further comprising filters connected to the signal amplifier and the data acquisition unit, respectively.
5. The device of any one of claims 1-3, wherein the first electrode and the second electrode are non-polarized electrodes.
6. The device according to claim 5, characterized in that the non-polarizing electrode is in particular a silver chloride electrode, a gold electrode, a platinum black electrode or a glass microelectrode.
7. A plant electric signal detection method based on the device of any one of claims 1 to 6, comprising:
alternately irradiating the plant to be detected in the insulating vessel by using light with the first preset illumination intensity and light with the second preset illumination intensity;
acquiring a first electric signal generated by the leaves of the plant to be detected and acquired by a data acquisition unit and a second electric signal generated by the roots of the plant to be detected;
and taking the difference between the first electric signal and the second electric signal as the detection result of the plant electric signal of the plant to be detected.
8. The method of claim 7, wherein the first predetermined illumination intensity and the second predetermined illumination intensity are both monochromatic or mixed wavelength light of a predetermined wavelength.
9. The method of claim 7, wherein the first predetermined illumination intensity and the second predetermined illumination intensity are both visible light.
10. The method according to any one of claims 7 to 9, further comprising, before the alternately irradiating the plant to be tested in the insulated container with the first preset light intensity and the second preset light intensity:
and if the height of the plant to be detected is lower than that of the insulating vessel, culturing the plant to be detected in the insulating vessel until the height of the plant to be detected reaches the height of the insulating vessel.
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| CN109959832B (en) * | 2019-04-25 | 2024-02-23 | 福建农林大学 | Plant electric signal acquisition platform and method |
| CN112305313A (en) * | 2020-10-29 | 2021-02-02 | 北京林业大学 | Plant leaf impedance spectrum measuring device and method based on AD5933 chip |
| CN113176301B (en) * | 2021-04-30 | 2024-04-30 | 中国科学院地球化学研究所 | Method for detecting plant health vigor based on electrophysiological rhythm |
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