CN112280893B - Molecular marker of wheat yield related gene TaD11-2A and application thereof - Google Patents
Molecular marker of wheat yield related gene TaD11-2A and application thereof Download PDFInfo
- Publication number
- CN112280893B CN112280893B CN202011479038.3A CN202011479038A CN112280893B CN 112280893 B CN112280893 B CN 112280893B CN 202011479038 A CN202011479038 A CN 202011479038A CN 112280893 B CN112280893 B CN 112280893B
- Authority
- CN
- China
- Prior art keywords
- tad11
- wheat
- molecular marker
- gene
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the field of plant variation or genetic engineering, and discloses a wheat yield related geneTaD11‑2AThe molecular marker is TaD11-ID3, specific primers of the molecular marker TaD11-ID3 are TaD11-ID3-1F and TaD11-ID3-1R, and the sequence of the TaD11-ID3-1R is shown as SEQ ID NO: 1, the TaD11-ID3-1F has a sequence shown in SEQ ID NO: 2, respectively. The invention is to dig from the wheat natural populationTaD11‑2AThe excellent allele of the gene provides a relevant functional molecular marker for identifying the wheat ear length, the number of spikelets and the number of grains per ear, and provides gene resources and an effective way for genetic improvement of wheat yield traits.
Description
Technical Field
The present invention belongs to a plant varietyThe field of XOR genetic engineering, in particular to a gene related to wheat yieldTaD11-2AAnd applications thereof.
Background
Wheat (A), (B)Triticum aestivumL.) is the most important food crop in the world, providing 20% energy to humans. The need to improve crop yields to accommodate global population increases and social growth is still the current mainstream (sentihold Asseng et al, 2020). The traditional breeding identification phenotype has large workload, high cost and overlong period, and the development of gene functional markers provides a reliable molecular marker toolbox for the genetic improvement of wheat and the development of new germplasm, thereby reducing the cost to the maximum extent (Rasheet et., 2016).
Brassinosteroids (BRs) are novel plant endogenous hormones, and play an important role in promoting crop growth and increasing crop yield.OsD11Is a key gene of BR biosynthesis pathway in rice, and research finds thatOsD11Can obviously improve the grain size and the yield of the single plant (Wu et al, 2016). However, wheat has not been found yetTaD11The functional research report of gene and the application of molecular marker.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a gene related to wheat yieldTaD11-2AThe molecular marker and the application thereof provide gene resources and effective ways for genetic improvement of wheat yield traits.
To achieve the above objects, one aspect of the present invention provides a gene related to wheat yieldTaD11-2AThe molecular marker adopts the following technical scheme:
wheat yield related geneTaD11-2AThe molecular marker is TaD11-ID3, specific primers of the molecular marker TaD11-ID3 are TaD11-ID3-1F and TaD11-ID3-1R, and the sequence of the TaD11-ID3-1R is shown as SEQ ID NO: 1, the TaD11-ID3-1F has a sequence shown in SEQ ID NO: 2, respectively.
In another aspect of the present invention, the wheat yield-related gene is providedTaD11-2AApplication of molecular marker in identification of wheat yield traits。
The invention also provides a wheat flourTaD11-2APrimers designed from genome sequences are used for amplification sequencing, and the primers are SeqD11-2A-1F and SeqD11-2A-1R, SeqD11-2A-2F and SeqD11-2A-2R, and the sequences of SeqD11-2A-1F, SeqD11-2A-1R, SeqD11-2A-2F and SeqD11-2A-2R are respectively shown as SEQ ID NO: 3-SEQ ID NO: and 6.
The above-mentionedTaD11-2AThere are 3 SNPs and 1 3-bp InDel in the gene region sequence, and 2 haplotypes Hapl a and Hapl b are present.
Further, theTaD11-2A3 SNPs in the gene region sequence and 1 3-bp InDel locus are completely cosegregated.
The above-mentionedTaD11-2AThe genome sequence is shown as SEQ ID NO: shown at 7.
The invention is to dig from the wheat natural populationTaD11-2AThe excellent allele of the gene provides a relevant functional molecular marker for identifying the wheat ear length, the number of spikelets and the number of grains per ear, and provides gene resources and an effective way for genetic improvement of wheat yield traits.
Drawings
FIG. 1 is a drawing ofTaD11-2ASchematic diagram of gene region variation;
FIG. 2 shows the partial detection results of TaD11-ID3 on denaturing polyacrylamide gel.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
The experimental procedures used in the following examples are conventional unless otherwise specified.
Example 1: wheat yield related geneTaD11-2AObtaining polymorphic sites
According to wheatTaD11-2ADesigning specific primers for genome sequence to carry out amplification sequencing, wherein the primers are used for amplification sequencingThe compounds are SeqD11-2A-1F and SeqD11-2A-1R, and SeqD11-2A-2F and SeqD11-2A-2R, wherein the sequences of SeqD11-2A-1F, SeqD11-2A-1R, SeqD11-2A-2F and SeqD11-2A-2R are respectively shown as SEQ ID NO: 3-SEQ ID NO: and 6.
The specific primer sequences are as follows:
SeqD11-2A-1F AATGATAGCTAGACAGAGAGGCA
SeqD11-2A-1R TCTGAAGGGTCACATATTTGGT
SeqD11-2A-2F TCCACCAAATATGTGACCCTT
SeqD11-2A-2R TTCATACATATCTCCCCATAACG
(1) the DNA of 60 wheat materials (all from the national germplasm resource pool) in Table 1 was amplified by PCR using specific primers (SeqD 11-2A-1F/1R, SeqD 11-2A-2F/2R) in a 50ml system comprising 5ml of DNA, 2.5ml of upstream primer, 2.5ml of downstream primer, 25ml of Phanta Max Super-Fidelity DNA Polymerase, 15ml of ddH20；
The method adopts a common amplification program for amplification and comprises the following steps:
1) denaturation is carried out for 5min at the temperature of 95 ℃,
2) common PCR program for 34 cycles: denaturation at 95 ℃ for 30s, renaturation at 58 ℃ for 30s, and extension at 72 ℃ for 2 min;
3) extending for 10 min at 72 ℃; after completion of amplification, the cells were stored at 4 ℃.
Table 160 parts of common wheat varieties
(2) And (3) performing gel cutting recovery on the target fragment, and performing bidirectional one-generation sequencing on the recovered product by utilizing SeqD11-2A-1F/1R, SeqD11-2A-2F/2R primers. Through the discovery of the sequence analysis, the method has the advantages of simple process,TaD11-2Athere are 3 SNPs and 1 3-bp InDel in the gene region sequence, and 2 haplotypes Hapl a and Hapl b in total (as shown in FIG. 1).
Example 2: wheat yield related geneTaD11-2ADevelopment of molecular markers and haplotype identification
(1) Development of molecular markers
TaD11-2AThe sequence of (1) is completely separated from 3 SNPs and 1 3-bp InDel locus, a new specific primer is designed according to the 3-bp InDel and is marked as TaD11-ID3, the specific primers of the molecular marker TaD11-ID3 are TaD11-ID3-1F and TaD11-ID3-1R, the sequence of the TaD11-ID3-1R is shown as SEQ ID NO: 1, the TaD11-ID3-1F and TaD11-ID3-1R have the sequences shown in SEQ ID NO: 2, the concrete steps are as follows:
TaD11-ID3-1F GCTCGTACCACCACACCAC
TaD11-ID3-1R CCCGCATTGCAAAGATCC
(2) identification of haplotypes
Selecting a natural population (table 2) consisting of 190 parts of common hexaploid wheat, and carrying out genotype identification on the natural population by using the molecular marker TaD11-ID3, wherein the method comprises the following steps:
1) performing PCR amplification on the DNA of the wheat variety to be detected by using a labeled primer, wherein the PCR amplification system is 10 mu l, and comprises the following steps: mu.l of DNA template, 0.5. mu.l of upstream primer, 0.5. mu.l of downstream primer, 5. mu.l of 2 XTAQQ PCR StarMix, 3. mu.l of ddH 2O;
the method adopts a common amplification program for amplification and comprises the following steps:
a) denaturation is carried out for 5min at the temperature of 95 ℃,
b) common PCR program for 34 cycles: denaturation at 95 deg.C for 30s, renaturation at 50 deg.C for 30s, and extension at 72 deg.C for 1min;
c) extending for 10 min at 72 ℃; after completion of amplification, the cells were stored at 4 ℃.
2) The 5% denatured polyacrylamide gel means that 100ml of polyacrylamide gel solution contains 4.75g of acrylamide, 0.25g of methylene acrylamide and 45g of urea, and the results of partial electrophoresis are shown in FIG. 2, and all the identification results are shown in Table 2.
TABLE 2 typing results for marker TaD11-ID3 in the Natural population
Example 3: association analysis of haplotype and yield traits
The GLM (PCA) model of software TASSEL 5.2 is utilized to combine genotype data (55K chip + TaD11-ID3 genotype) and phenotype data of natural population under 2 years (2018-. As a result, it was found that the ear length, the number of spikelets and the number of grains per ear of the Hapl b type were significantly increased (P < 0.05) relative to the Hapl a type (Table 3), and the average increase ratios were 8.5% (ear length), 5.8% (number of spikelets) and 8.0% (number of grains per ear), respectively. The above results show that Hapl b type isTaD11-2AThe excellent genotype of the gene, TaD11-ID3 marker, can play a role in breeding wheat with excellent yield traits.
The above-mentionedTaD11-2AThe genome sequence is shown as SEQ ID NO: shown at 7.
TABLE 3TaD11-2AGene haplotype and yield character correlation analysis result
Note: p < 0.01, P < 0.05.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention.
<110> university of Ludong
<120> molecular marker of wheat yield related gene tad11-2a and application thereof
<160> 7
<170> SIPOSequenceListing 1.0
<210> 1
<211> 19
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 1
gctcgtacca ccacaccac 19
<210> 2
<211> 18
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 2
cccgcattgc aaagatcc 18
<210> 3
<211> 26
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 3
aatgata gctagacaga gaggca 23
<210> 4
<211> 26
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 4
tctgaa gggtcacata tttggt 22
<210> 5
<211> 24
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 5
tccacca aatatgtgac cctt 21
<210> 6
<211> 27
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 6
ttcata catatctccc cataacg 23
<210> 7
<211> 4049
<212> DNA
<213> tad11-2a(tad11-2a)
<400> 7
gagagagaga agcagagggc gagcaagtga atgatagcta gacagagagg caaggcatgg 60
caggctccat gcagcttgtg agatgtaact aggtagggtg gggcggcggc ggcggcggtg 120
gtgtggtggt acgagcatga tggtgggggt gggcgatctt gtgctggctg ctccggcgat 180
cctgctcgcc ttgctcctga ctctggtgct gagccacttc ctgcctttgc tcctcaaccc 240
caaggctccg aggggcagct ttggatggcc ccttgtcggc gagaccctca ggttcctcac 300
ccctcacgcc tccaacacgc tgggtagctt cctggaggat cactgctcca ggtactttta 360
ttttcttctt cttttttgtt atcattcacc acagccacac cacacctcaa gatttctcca 420
cgatgcaatc aagcacgtag tattgtttat catttactca tgtggcagca aaagatgatg 480
catgtcgtgc atgttgtcct gactcagcac ctttatcttc ctgtctttgc tccaaggctg 540
tgatatattt gaatttctac aggttttgtg cacagaacct gtttctgaaa aagttttacc 600
tattgttttg tgggggagag aggatggaaa gatctcataa gaccccctat caaatgatgg 660
caacttaaca acatgaaaaa agatctaaca ttgcagttga tgtgatgatg atgcaggtat 720
gggagggtgt tcaagtccca tctgttctgc acccccaccg tagtgtcctg tgaccaggag 780
ctgaaccact tcatcctgca gaatgaggag aggttgttcc agtgcagcta ccccaggcca 840
attcatggca tactgggcaa gtcctccatg ctggtggtcc tcggggagga ccacaagcgg 900
ctcaggaacc ttgctctggc cctcgtcacc tccaccaagc tcaagccaag ctaccttggc 960
gacattgaga ggattgcgct gcacatagtc gggtcatggc acggcaaggg cggcagcatc 1020
accttctgcg aggaggcaag aaaggttggt ctccccgtgc atgcactaac tacaaccaaa 1080
gctggctagg catgcatcag tagggacatg atcccgtcaa acatggacga aaaactctgc 1140
acatgttacc tatggctaca tcatcatgct gttacttgct gatgcatcat cacatatttt 1200
ttcaaacgct ggctttgact taccagggtc attcttctcc gtattttgac tctctttttc 1260
tgactttttt tttcctttgg cacagtttgc gttcagtgtg atagtgaagc aggtgctggg 1320
gctgtcgcca gaggagcctg tcactgccat gatactggag gacttcctca ccttcatgaa 1380
gggcctcatc tctttccctc tccgcatccc agggacccca tacgccaaag ctgtccaggt 1440
aactaagtaa acaactctgg aataccacct tcttaaactg tcaaagtttc cagcacttct 1500
tatctacttc accatgcatc aggctcatga aactctggta tatattgttc taacactgac 1560
tgaggttctt gggtatgccg atcattcctc acaggccaga gagaggatat caagcactgt 1620
gaagggcatt atcgaggaga ggaggaaggc tgactgctgc aagagggatg atttcctcaa 1680
cgtgctcctg tcaaccgatg aactctctga cgaggagaaa gtgagctttg tgctggactc 1740
cctgctagga ggatacgaga ccacctcact catgatctcc atggtcgtct acttccttgg 1800
gcagtcagct caagatctgg acctagtgaa ggtacataca tacctgatca agcttaagcc 1860
acaggtgatt ggtggttgcg tgcatgtgtc acttattata tatgaacatg ctgtgcatgc 1920
agagggagca tcaaggcata agatctacca aagcaaagga ggagtgcttg agctctgagg 1980
actacaagaa gatggaatat acccaacatg taagtaagat gatgatcatt cagtacacgc 2040
tttaggatat gtagccggtt ggtaagcagg gatccttttt tctttctatt atcagacatc 2100
caccaaatat gtgacccttc agaatttatg ttttttctgt ccgtatgcct gcctatatct 2160
ggaattttga ctagagcaaa caacactttg caagaacagt gcaaatgggg aaggaagggt 2220
ctcttaattt gttctgtaat aattggaatg gttcaatgat acatacatac atgcgtggat 2280
gtcctttcag gttatcaatg aggcgctgag atgtggaaac attgtcaagt ttgtccacag 2340
gaaggccctc aaagatgtca gatacaaagg taattaaccc tgctagtact tggggctttc 2400
ccagcagatc ccattgtgct catcacatga taatggatca ggcaaagggg gcggttgggc 2460
ctttattgct gatttgtgag cattgttatt cctaaacacc tagcttttga tagaagatgc 2520
tcaaaagcaa gtggacctct tcttttgctc tgttgcagag tatctgattc catctggctg 2580
gaaggtccta cctgttttca gtgctgttca tttgaacccc tcgcttcatg gaaatgccca 2640
acagtttcag ccttgcagat gggaggtatg tctgatttcc ataccatgtg cacgggcatc 2700
tatatttgta gtcaagtaat gaatacatct tagtttatgc agattagctt taatataata 2760
tgcttgttta gggaagccta actttgacat aatatgcttt ttatgaacta ttttatacaa 2820
tgaaaaggtg agaacaaatc agaagagctt atctgtagct ttgagagaat cttctttaag 2880
gttgctctgt taactaattg atgcctttaa ttagtgagtg tccaactaac ccatgccata 2940
ataattactt gcaactatcc tggtatcttc agatgccaag caggctttgg ctgagggcct 3000
agtgccttct gtacatggtc ctaaccatga ttcctcactc aaagccagca tccttggctt 3060
tgcttcttca ttttcttaac tcccttgatg aagctagctc atatgcatga cttgtacaat 3120
tgattgatgc aacagggccc aagccaaggt acaagcaaga agtttacacc attcggcggt 3180
ggcaccaggc tctgccctgg atcagagctt gccaaagtag aggctgcttt cttcctccat 3240
caccttgtgc tcaatttcag gtaaaacatt tctcctagcc gattaagaga tacacatgta 3300
gcaactaacg cagcaagagc aacaaatcgg agaaacgaag aacctaaata ttttgtaagg 3360
ataaatcaac ttaagaatga aattaaccag aggaaatatg aatagcatga ccacagattg 3420
atgcagtctc atactagtgg agatgctctg caaacatggc agcatggagt atccaataag 3480
tatggcatgc aagttgagtg gtaccaaatc tggtccatag atatctgcag ttatcagatt 3540
gtgacagaag aaaatgcatc tccctctact ttttttcaga gcaaggagat aagtgcttac 3600
tgaatctcta tagttactga tcatggtata atttggcacc ctgcagatgg aaaatcgatg 3660
gcgatgacat tccaatggca tacccgtacg tggagttccc gagaggcctg ccgatagaaa 3720
tcgagccgat ttgctctgaa tcttgactgg gttcttgcag ctgcaaacat ggcggtgagt 3780
gaaaaaagag attgcaagca gtgaagcgaa gaagcaccga tgactagctt agcttagcta 3840
gttcctggaa tggggtagct gaggaggttg tgatctcctc tgtacatggt gtacatatat 3900
tttgtccatt gtgattactt gaggtggttg ggtcaccctg ttacatcctc cactgtctgg 3960
actactatcc tatagaaaaa aaatgggctc ctggtgttta catttacttc tgaaattcgt 4020
tatggggaga tatgtatgaa ccggacctc 4049
Claims (4)
1. Wheat yield related geneTaD11-2AThe molecular marker is TaD11-ID3, specific primers of the molecular marker TaD11-ID3 are TaD11-ID3-1F and TaD11-ID3-1R, and the sequence of the TaD11-ID3-1R is shown as SEQ ID NO: 1, the TaD11-ID3-1F has a sequence shown in SEQ ID NO: 2 is shown in the specification;
the above-mentionedTaD11-2AThe genome sequence is shown as SEQ ID NO: shown at 7.
2. Wheat yield-related gene according to claim 1TaD11-2AThe molecular marker of (a) is applied to identification of wheat yield traits, wherein the wheat yield traits comprise wheat spike length, number of spikelets and number of grains per spike.
3. As shown in SEQ ID NO: 7 is shown inTaD11-2AGene characterized by the presence of 3 SNPs and 1 3-bp InDel, for a total of 2 haplotypes, Hapl a and Hapl b.
4. The method of claim 3TaD11-2AThe gene of (1), wherein 3 SNPs and 1 SNP in the sequenceThe 3-bp InDel sites were completely cosegregated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011479038.3A CN112280893B (en) | 2020-12-16 | 2020-12-16 | Molecular marker of wheat yield related gene TaD11-2A and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011479038.3A CN112280893B (en) | 2020-12-16 | 2020-12-16 | Molecular marker of wheat yield related gene TaD11-2A and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112280893A CN112280893A (en) | 2021-01-29 |
| CN112280893B true CN112280893B (en) | 2021-04-23 |
Family
ID=74425943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011479038.3A Active CN112280893B (en) | 2020-12-16 | 2020-12-16 | Molecular marker of wheat yield related gene TaD11-2A and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112280893B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116334301B (en) * | 2023-05-29 | 2023-08-11 | 鲁东大学 | Molecular marker closely linked with major QTL of wheat spike length and application thereof |
| CN116377121B (en) * | 2023-06-05 | 2023-08-18 | 鲁东大学 | Molecular marker related to wheat flag leaf length and spike length and application thereof |
| CN116926233B (en) * | 2023-09-13 | 2023-12-12 | 鲁东大学 | Molecular marker of wheat TaHAL3-7B gene and application thereof |
| CN118086578B (en) * | 2024-04-28 | 2024-06-25 | 鲁东大学 | Molecular marker of wheat biological rhythm clock gene TaPRR-B1 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349542A (en) * | 2015-12-07 | 2016-02-24 | 安徽农业大学 | Indel molecular marker related to wheat thousand seed weights and application |
| CN110982933A (en) * | 2020-03-02 | 2020-04-10 | 鲁东大学 | Molecular marker closely linked with major QTL (quantitative trait locus) of wheat grain length and application thereof |
| CN111304351A (en) * | 2020-02-14 | 2020-06-19 | 山西省农业科学院生物技术研究中心 | Method for screening wheat with different grain number per ear and single plant yield and kit used by method |
-
2020
- 2020-12-16 CN CN202011479038.3A patent/CN112280893B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349542A (en) * | 2015-12-07 | 2016-02-24 | 安徽农业大学 | Indel molecular marker related to wheat thousand seed weights and application |
| CN111304351A (en) * | 2020-02-14 | 2020-06-19 | 山西省农业科学院生物技术研究中心 | Method for screening wheat with different grain number per ear and single plant yield and kit used by method |
| CN110982933A (en) * | 2020-03-02 | 2020-04-10 | 鲁东大学 | Molecular marker closely linked with major QTL (quantitative trait locus) of wheat grain length and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| CLUSTERED PRIMARY BRANCH 1, a new allele of DWARF11, controls panicle architecture and seed size in rice;Wu Yongzhen 等;《Plant biotechnology journal》;20160130;第14卷(第1期);全文 * |
| 小麦株高相关基因TaDwarf11的克隆与功能分析;赵毅.;《中国优秀硕士学位论文全文数据库 农业科技辑》;20190815;第二章第13页第2.2.1节及图 2-1 * |
| 小麦油菜素内酯合成基因TaD11的功能分析;徐彗渊 等;《第十届全国小麦基因组学及分子育种大会摘要集》;20190811(第1期);摘要 * |
| 小麦穗粒数主效QTL-qKnps-4A遗传效应解析;崔俊鹏 等;《分子植物育种》;20180908;第17卷(第11期);第3632-3640页 * |
| 赵毅..小麦株高相关基因TaDwarf11的克隆与功能分析.《中国优秀硕士学位论文全文数据库 农业科技辑》.2019,D047-53页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112280893A (en) | 2021-01-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112280893B (en) | Molecular marker of wheat yield related gene TaD11-2A and application thereof | |
| CN109913572B (en) | Molecular marker tightly linked with spike length major QTL (quantitative trait locus) and application thereof | |
| CN116926233B (en) | Molecular marker of wheat TaHAL3-7B gene and application thereof | |
| CN110042171B (en) | Method for identifying wheat yield traits and related molecular markers | |
| CN113699269B (en) | SNP (single nucleotide polymorphism) site related to small spike number per spike and spike grain number characters of wheat and application thereof | |
| Shu et al. | SNPs discovery and CAPS marker conversion in soybean | |
| CN116377121B (en) | Molecular marker related to wheat flag leaf length and spike length and application thereof | |
| CN117403003B (en) | Molecular marker of wheat TaCHLI-7B gene and application thereof | |
| CN111850161B (en) | Sand-melt aegilops 7SshChromosome specific molecular marker and application thereof | |
| CN109797242B (en) | Molecular marker and method for identifying wheat yield-related traits | |
| CN109234423B (en) | Application of soybean molecular marker and polymorphism thereof in identification of soybean branch number traits | |
| CN113699268B (en) | Wheat thousand grain weight character related SNP site and application thereof | |
| CN117385098B (en) | Molecular marker related to wheat grain density and application thereof | |
| CN109811074B (en) | Marker for auxiliary detection of high single-ear and small-ear number characters of wheat and application of marker | |
| CN113308554B (en) | SNP molecular marker related to bovine growth traits and application thereof | |
| CN113621733A (en) | Wheat spike number per spike and drought-resistant character related SNP site and application thereof | |
| CN117025832A (en) | Molecular marker for auxiliary detection of soybean oil content and application thereof | |
| CN113699267B (en) | SNP locus related to wheat plant height traits and application thereof | |
| CN106222268B (en) | POU1F1 haplotype molecular marker related to duck growth traits and application | |
| CN114317807A (en) | Wheat thousand grain weight character related SNP site and application thereof | |
| CN118086577B (en) | Molecular marker of wheat biological rhythm clock gene TaPRR-A1 and application thereof | |
| CN112430681A (en) | Molecular marker for identifying wheat plant height and yield traits and application thereof | |
| CN106636386B (en) | Molecular marker DNdCAPS8.03-1 linked with maize head smut resistant minor major site and application thereof | |
| CN110484651B (en) | Molecular marker in wheat yield related gene TaNRT2-6D and application thereof | |
| CN116732230B (en) | Rice nitrogen efficient InDel molecular marker GRF4M1 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |