CN112279455A - Microbial treatment method for landscape water body eutrophication - Google Patents
Microbial treatment method for landscape water body eutrophication Download PDFInfo
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- CN112279455A CN112279455A CN202011013587.1A CN202011013587A CN112279455A CN 112279455 A CN112279455 A CN 112279455A CN 202011013587 A CN202011013587 A CN 202011013587A CN 112279455 A CN112279455 A CN 112279455A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000000813 microbial effect Effects 0.000 title claims abstract description 25
- 238000012851 eutrophication Methods 0.000 title claims abstract description 22
- 239000000126 substance Substances 0.000 claims abstract description 26
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000011574 phosphorus Substances 0.000 claims abstract description 23
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 23
- 239000002131 composite material Substances 0.000 claims abstract description 21
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 17
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 17
- 238000000746 purification Methods 0.000 claims abstract description 12
- 238000005067 remediation Methods 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 8
- 239000010452 phosphate Substances 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims description 35
- 239000013078 crystal Substances 0.000 claims description 30
- 241000195493 Cryptophyta Species 0.000 claims description 20
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 20
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 20
- 241000196324 Embryophyta Species 0.000 claims description 15
- 239000011573 trace mineral Substances 0.000 claims description 14
- 235000013619 trace mineral Nutrition 0.000 claims description 14
- 244000063299 Bacillus subtilis Species 0.000 claims description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 10
- 206010016654 Fibrosis Diseases 0.000 claims description 10
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 10
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 10
- 241000108664 Nitrobacteria Species 0.000 claims description 10
- 241000187654 Nocardia Species 0.000 claims description 10
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 10
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 10
- 230000002353 algacidal effect Effects 0.000 claims description 10
- 239000001110 calcium chloride Substances 0.000 claims description 10
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 10
- 230000004761 fibrosis Effects 0.000 claims description 10
- 238000011049 filling Methods 0.000 claims description 10
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 10
- 238000009630 liquid culture Methods 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 10
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 10
- 239000001632 sodium acetate Substances 0.000 claims description 10
- 235000017281 sodium acetate Nutrition 0.000 claims description 10
- 239000004317 sodium nitrate Substances 0.000 claims description 10
- 235000010344 sodium nitrate Nutrition 0.000 claims description 10
- 244000005700 microbiome Species 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 5
- 241000628997 Flos Species 0.000 claims description 5
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 5
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 5
- 241000192710 Microcystis aeruginosa Species 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229920002494 Zein Polymers 0.000 claims description 5
- 230000003213 activating effect Effects 0.000 claims description 5
- 238000009388 chemical precipitation Methods 0.000 claims description 5
- 229930002868 chlorophyll a Natural products 0.000 claims description 5
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 5
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 5
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 5
- 229960001484 edetic acid Drugs 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 239000011565 manganese chloride Substances 0.000 claims description 5
- 235000002867 manganese chloride Nutrition 0.000 claims description 5
- 229940099607 manganese chloride Drugs 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 239000005019 zein Substances 0.000 claims description 5
- 229940093612 zein Drugs 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 10
- 239000000945 filler Substances 0.000 abstract description 8
- 230000032770 biofilm formation Effects 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 description 4
- 239000010802 sludge Substances 0.000 description 4
- 238000000053 physical method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/58—Treatment of water, waste water, or sewage by removing specified dissolved compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a microbial treatment method for landscape water body eutrophication, which comprises the following steps: step one, chemical phosphorus removal, namely adding a chemical phosphorus removal agent into a landscape water body; step two, microbial purification, namely adding a composite microbial remediation microbial inoculum to the landscape water body; and step three, performing auxiliary purification on the aquatic plants, performing filler biofilm culturing, placing the biofilm-cultured filler on the lower part of the floating bed, and placing the biological floating bed on the landscape water body to be treated. The invention organically combines a chemical method and an ecological method, and utilizes the advantage of quick effect taking of the chemical method to quickly remove phosphate in the water body, so that flora which plays a main role in subsequently added microbial inoculum and filler biofilm formation can quickly establish the advantage, the water body is quickly and continuously treated, the occurrence of eutrophication is avoided, and the proportion of the flora in the microbial inoculum and the filler biofilm formation is reasonable and quick effect taking is realized.
Description
Technical Field
The invention relates to the technical field of landscape water body treatment, in particular to a microbial treatment method for landscape water body eutrophication.
Background
Most of landscape water in cities is artificially constructed water, most of landscape water is in a closed or semi-closed state, the flowability is poor, the flow is small, and under the condition that the conditions such as pollutants, temperature, illumination and the like are all suitable, algae and microorganisms in the water are propagated in a large quantity, so that the water is in a eutrophication state, the conditions that the water is low in transparency, serious in peculiar smell and even hard to recover, such as aquatic animal death and the like, are easily caused.
At present, the method for treating landscape water eutrophication mainly comprises three major types, namely a physical method, a chemical method and an ecological method, wherein the physical method can only remove the existing algae by using a physical method, and although the effect is quick, the water body still recovers the eutrophication state in a short time; the chemical method can obtain obvious algae removal effect in a short time and improve the transparency of the water body, but can not remove nutrient substances such as nitrogen, phosphorus and the like out of the water body, and the low-concentration algae removal agent can cause the algae to generate drug resistance after long-term use; although the ecological method can solve the problem of water eutrophication fundamentally, the treatment effect is not good due to unreasonable flora proportion, and the defect of slow effect is also existed.
Disclosure of Invention
The invention aims to solve the defects of the prior art and provides a microbial treatment method for landscape water body eutrophication.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microbial treatment method for landscape water body eutrophication comprises the following specific steps:
step one, chemical phosphorus removal,
adding a chemical phosphorus removal agent into the landscape water body, and removing phosphate in the water body through chemical precipitation and adsorption, wherein the chemical phosphorus removal agent is polyaluminium chloride or a phosphorus locking agent;
step two, the microorganism purification is carried out,
adding a composite microbial remediation microbial inoculum to the landscape water body, wherein the composite microbial remediation microbial inoculum comprises the following components in parts by weight: 10-30 parts of wheat bran, 6-10 parts of zein, 40-60 parts of diatomite and 8-10 parts of composite bacteria; wherein the composite bacteria comprise rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, nocardia fibrosis, lactobacillus plantarum and bacillus subtilis, and the mixing mass ratio of the rhodopseudomonas palustris, the nitrobacteria, the denitrifying bacteria, the nocardia fibrosis, the lactobacillus plantarum and the bacillus subtilis is as follows: 2-4: 2-4: 1-3: 2-6: 4-6: 1-4;
step three, the aquatic plant assists in purifying,
activating denitrifying algicidal bacteria in a water distribution culture solution, then filling and filming, placing aquatic plants on the upper part of a floating bed, placing the filmed filling on the lower part of the floating bed to prepare a biological floating bed, placing the biological floating bed on a landscape water body to be treated, and performing auxiliary purification on the water body.
Further, the formula of the water-distributed culture solution in the third step is as follows: 1.0g/L of sodium acetate, 0.2g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystal, 0.05g/L of calcium chloride and 2ml/L of trace element solution.
Further, the preparation method of the denitrifying algicidal bacterium in the third step comprises the following steps:
mixing the bottom mud of the landscape water to be treated, the water sample and microcystis aeruginosa, adding the mixture into an enrichment medium for culturing, replacing part of the enrichment medium every 4-5 days, indirectly representing the number of algae cells through chlorophyll a, and culturing the bottom mud of the product in a liquid culture solution I when the algae dissolving efficiency is more than 50% to obtain the denitrifying algae dissolving bacteria.
Further, the formula of the liquid culture solution I is as follows: 0.5g/L of sodium acetate, 0.1g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystals, 0.05g/L of calcium chloride and 2ml of trace element solution.
Further, the formula of the trace element solution is as follows: 1.0g/L of ethylene diamine tetraacetic acid, 0.2g/L of zinc sulfate, 0.1g/L of manganese chloride crystal, 0.5g/L of ferrous sulfate crystal, 0.5g/L of copper sulfate crystal and 0.2g/L of cobalt chloride crystal.
Further, the aquatic plants in step three include: one or more of rhizoma Iridis Tectori, medulla Junci, rhizoma Acori Calami, herba Apii, rhizoma Sparganii, rhizoma Phragmitis, and flos Nelumbinis.
The invention has the beneficial effects that: the invention organically combines a chemical method and an ecological method, and utilizes the advantage of quick effect taking of the chemical method to quickly remove phosphate in the water body, so that flora which plays a main role in subsequently added microbial inoculum and filler biofilm formation can quickly establish the advantage, the water body is quickly and continuously treated, the occurrence of eutrophication is avoided, and the proportion of the flora in the microbial inoculum and the filler biofilm formation is reasonable and quick effect taking is realized.
Detailed Description
The invention is further illustrated by the following examples:
example one
A microbial treatment method for landscape water body eutrophication comprises the following specific steps:
step one, chemical phosphorus removal,
adding a chemical phosphorus removal agent into the landscape water body, and removing phosphate in the water body through chemical precipitation and adsorption, wherein the chemical phosphorus removal agent is polyaluminium chloride or a phosphorus locking agent;
step two, the microorganism purification is carried out,
adding a composite microbial remediation microbial inoculum to the landscape water body, wherein the composite microbial remediation microbial inoculum comprises the following components in parts by weight: 10 parts of wheat bran, 6 parts of zein, 40 parts of diatomite and 8 parts of composite bacteria; wherein the composite bacteria comprise rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, nocardia fibrosis, lactobacillus plantarum and bacillus subtilis, and the mixing mass ratio of the rhodopseudomonas palustris, the nitrobacteria, the denitrifying bacteria, the nocardia fibrosis, the lactobacillus plantarum and the bacillus subtilis is as follows: 2: 2: 1: 2: 4: 1;
step three, the aquatic plant assists in purifying,
activating denitrifying algicidal bacteria in a water distribution culture solution, then filling and filming, placing aquatic plants on the upper part of a floating bed, placing the filmed filling on the lower part of the floating bed to prepare a biological floating bed, placing the biological floating bed on a landscape water body to be treated, and performing auxiliary purification on the water body.
Further, the formula of the water-distributed culture solution in the third step is as follows: 1.0g/L of sodium acetate, 0.2g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystal, 0.05g/L of calcium chloride and 2ml/L of trace element solution.
Further, the preparation method of the denitrifying algicidal bacterium in the third step comprises the following steps:
mixing the bottom sludge of landscape water to be treated, a water sample and microcystis aeruginosa, adding the mixture into an enrichment medium for culture, replacing part of the enrichment medium every 4 days, indirectly representing the number of algae cells through chlorophyll a, and culturing the bottom sludge of a product in a liquid culture solution I when the algae dissolving efficiency is more than 50 percent to obtain the denitrifying algae dissolving bacteria.
Further, the formula of the liquid culture solution I is as follows: 0.5g/L of sodium acetate, 0.1g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystals, 0.05g/L of calcium chloride and 2ml of trace element solution.
Further, the formula of the trace element solution is as follows: 1.0g/L of ethylene diamine tetraacetic acid, 0.2g/L of zinc sulfate, 0.1g/L of manganese chloride crystal, 0.5g/L of ferrous sulfate crystal, 0.5g/L of copper sulfate crystal and 0.2g/L of cobalt chloride crystal.
Further, the aquatic plants in step three include: one or more of rhizoma Iridis Tectori, medulla Junci, rhizoma Acori Calami, herba Apii, rhizoma Sparganii, rhizoma Phragmitis, and flos Nelumbinis.
Example two
A microbial treatment method for landscape water body eutrophication comprises the following specific steps:
step one, chemical phosphorus removal,
adding a chemical phosphorus removal agent into the landscape water body, and removing phosphate in the water body through chemical precipitation and adsorption, wherein the chemical phosphorus removal agent is polyaluminium chloride or a phosphorus locking agent;
step two, the microorganism purification is carried out,
adding a composite microbial remediation microbial inoculum to the landscape water body, wherein the composite microbial remediation microbial inoculum comprises the following components in parts by weight: 30 parts of wheat bran, 10 parts of zein, 60 parts of diatomite and 10 parts of composite bacteria; wherein the composite bacteria comprise rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, nocardia fibrosis, lactobacillus plantarum and bacillus subtilis, and the mixing mass ratio of the rhodopseudomonas palustris, the nitrobacteria, the denitrifying bacteria, the nocardia fibrosis, the lactobacillus plantarum and the bacillus subtilis is as follows: 4: 4: 3: 6: 6: 4;
step three, the aquatic plant assists in purifying,
activating denitrifying algicidal bacteria in a water distribution culture solution, then filling and filming, placing aquatic plants on the upper part of a floating bed, placing the filmed filling on the lower part of the floating bed to prepare a biological floating bed, placing the biological floating bed on a landscape water body to be treated, and performing auxiliary purification on the water body.
Further, the formula of the water-distributed culture solution in the third step is as follows: 1.0g/L of sodium acetate, 0.2g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystal, 0.05g/L of calcium chloride and 2ml/L of trace element solution.
Further, the preparation method of the denitrifying algicidal bacterium in the third step comprises the following steps:
mixing the bottom mud of the landscape water to be treated, the water sample and microcystis aeruginosa, adding the mixture into an enrichment medium for culturing, replacing part of the enrichment medium every 4-5 days, indirectly representing the number of algae cells through chlorophyll a, and culturing the bottom mud of the product in a liquid culture solution I when the algae dissolving efficiency is more than 50% to obtain the denitrifying algae dissolving bacteria.
Further, the formula of the liquid culture solution I is as follows: 0.5g/L of sodium acetate, 0.1g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystals, 0.05g/L of calcium chloride and 2ml of trace element solution.
Further, the formula of the trace element solution is as follows: 1.0g/L of ethylene diamine tetraacetic acid, 0.2g/L of zinc sulfate, 0.1g/L of manganese chloride crystal, 0.5g/L of ferrous sulfate crystal, 0.5g/L of copper sulfate crystal and 0.2g/L of cobalt chloride crystal.
Further, the aquatic plants in step three include: one or more of rhizoma Iridis Tectori, medulla Junci, rhizoma Acori Calami, herba Apii, rhizoma Sparganii, rhizoma Phragmitis, and flos Nelumbinis.
EXAMPLE III
A microbial treatment method for landscape water body eutrophication comprises the following specific steps:
step one, chemical phosphorus removal,
adding a chemical phosphorus removal agent into the landscape water body, and removing phosphate in the water body through chemical precipitation and adsorption, wherein the chemical phosphorus removal agent is polyaluminium chloride or a phosphorus locking agent;
step two, the microorganism purification is carried out,
adding a composite microbial remediation microbial inoculum to the landscape water body, wherein the composite microbial remediation microbial inoculum comprises the following components in parts by weight: 20 parts of wheat bran, 8 parts of zein, 50 parts of diatomite and 9 parts of composite bacteria; wherein the composite bacteria comprise rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, nocardia fibrosis, lactobacillus plantarum and bacillus subtilis, and the mixing mass ratio of the rhodopseudomonas palustris, the nitrobacteria, the denitrifying bacteria, the nocardia fibrosis, the lactobacillus plantarum and the bacillus subtilis is as follows: 3: 3: 2: 3: 5: 2;
step three, the aquatic plant assists in purifying,
activating denitrifying algicidal bacteria in a water distribution culture solution, then filling and filming, placing aquatic plants on the upper part of a floating bed, placing the filmed filling on the lower part of the floating bed to prepare a biological floating bed, placing the biological floating bed on a landscape water body to be treated, and performing auxiliary purification on the water body.
Further, the formula of the water-distributed culture solution in the third step is as follows: 1.0g/L of sodium acetate, 0.2g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystal, 0.05g/L of calcium chloride and 2ml/L of trace element solution.
Further, the preparation method of the denitrifying algicidal bacterium in the third step comprises the following steps:
mixing the bottom sludge of landscape water to be treated, a water sample and microcystis aeruginosa, adding the mixture into an enrichment medium for culture, replacing part of the enrichment medium every 4 days, indirectly representing the number of algae cells through chlorophyll a, and culturing the bottom sludge of a product in a liquid culture solution I when the algae dissolving efficiency is more than 50 percent to obtain the denitrifying algae dissolving bacteria.
Further, the formula of the liquid culture solution I is as follows: 0.5g/L of sodium acetate, 0.1g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystals, 0.05g/L of calcium chloride and 2ml of trace element solution.
Further, the formula of the trace element solution is as follows: 1.0g/L of ethylene diamine tetraacetic acid, 0.2g/L of zinc sulfate, 0.1g/L of manganese chloride crystal, 0.5g/L of ferrous sulfate crystal, 0.5g/L of copper sulfate crystal and 0.2g/L of cobalt chloride crystal.
Further, the aquatic plants in step three include: one or more of rhizoma Iridis Tectori, medulla Junci, rhizoma Acori Calami, herba Apii, rhizoma Sparganii, rhizoma Phragmitis, and flos Nelumbinis.
The invention organically combines a chemical method and an ecological method, and utilizes the advantage of quick effect taking of the chemical method to quickly remove phosphate in the water body, so that flora which plays a main role in subsequently added microbial inoculum and filler biofilm formation can quickly establish the advantage, the water body is quickly and continuously treated, the occurrence of eutrophication is avoided, and the proportion of the flora in the microbial inoculum and the filler biofilm formation is reasonable and quick effect taking is realized.
The present invention has been described in connection with the specific embodiments, and it is obvious that the specific implementation of the present invention is not limited by the above-mentioned manner, and it is within the protection scope of the present invention as long as various modifications are made by using the method concept and technical solution of the present invention, or the present invention is directly applied to other occasions without modification.
Claims (6)
1. A microbial treatment method for landscape water body eutrophication is characterized by comprising the following specific steps:
step one, chemical phosphorus removal,
adding a chemical phosphorus removal agent into the landscape water body, and removing phosphate in the water body through chemical precipitation and adsorption, wherein the chemical phosphorus removal agent is polyaluminium chloride or a phosphorus locking agent;
step two, the microorganism purification is carried out,
adding a composite microbial remediation microbial inoculum to the landscape water body, wherein the composite microbial remediation microbial inoculum comprises the following components in parts by weight: 10-30 parts of wheat bran, 6-10 parts of zein, 40-60 parts of diatomite and 8-10 parts of composite bacteria; wherein the composite bacteria comprise rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, nocardia fibrosis, lactobacillus plantarum and bacillus subtilis, and the mixing mass ratio of the rhodopseudomonas palustris, the nitrobacteria, the denitrifying bacteria, the nocardia fibrosis, the lactobacillus plantarum and the bacillus subtilis is as follows: 2-4: 2-4: 1-3: 2-6: 4-6: 1-4;
step three, the aquatic plant assists in purifying,
activating denitrifying algicidal bacteria in a water distribution culture solution, then filling and filming, placing aquatic plants on the upper part of a floating bed, placing the filmed filling on the lower part of the floating bed to prepare a biological floating bed, placing the biological floating bed on a landscape water body to be treated, and performing auxiliary purification on the water body.
2. The microbial treatment method for eutrophication of landscape water bodies according to claim 1, wherein the formula of the water distribution culture solution in the third step is as follows: 1.0g/L of sodium acetate, 0.2g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystal, 0.05g/L of calcium chloride and 2ml/L of trace element solution.
3. The method for treating the eutrophication microorganisms in the landscape water bodies according to claim 2, wherein the method for preparing the denitrifying algicidal bacteria in the third step is as follows:
mixing the bottom mud of the landscape water to be treated, the water sample and microcystis aeruginosa, adding the mixture into an enrichment medium for culturing, replacing part of the enrichment medium every 4-5 days, indirectly representing the number of algae cells through chlorophyll a, and culturing the bottom mud of the product in a liquid culture solution I when the algae dissolving efficiency is more than 50% to obtain the denitrifying algae dissolving bacteria.
4. The method for treating the eutrophication microorganisms in the landscape water bodies according to claim 3, wherein the formula of the liquid culture solution I is as follows: 0.5g/L of sodium acetate, 0.1g/L of sodium nitrate, 0.1g/L of monopotassium phosphate, 0.05g/L of magnesium sulfate crystals, 0.05g/L of calcium chloride and 2ml of trace element solution.
5. The microbial treatment method for eutrophication of landscape water bodies according to any one of claims 2 to 4, wherein the formula of the microelement solution is as follows: 1.0g/L of ethylene diamine tetraacetic acid, 0.2g/L of zinc sulfate, 0.1g/L of manganese chloride crystal, 0.5g/L of ferrous sulfate crystal, 0.5g/L of copper sulfate crystal and 0.2g/L of cobalt chloride crystal.
6. The method for microbial remediation of landscape water eutrophication of claim 1, wherein the aquatic plants in step three include: one or more of rhizoma Iridis Tectori, medulla Junci, rhizoma Acori Calami, herba Apii, rhizoma Sparganii, rhizoma Phragmitis, and flos Nelumbinis.
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