CN112263861A - Sample cup, cell pretreatment method and sample slide device - Google Patents
Sample cup, cell pretreatment method and sample slide device Download PDFInfo
- Publication number
- CN112263861A CN112263861A CN202011074173.XA CN202011074173A CN112263861A CN 112263861 A CN112263861 A CN 112263861A CN 202011074173 A CN202011074173 A CN 202011074173A CN 112263861 A CN112263861 A CN 112263861A
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- cavity
- cup
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- sample
- filter screen
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- 238000002203 pretreatment Methods 0.000 title abstract description 7
- 238000005192 partition Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000005070 sampling Methods 0.000 claims description 27
- 125000006850 spacer group Chemical group 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000003761 preservation solution Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims 7
- 239000012488 sample solution Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 abstract description 6
- 239000012212 insulator Substances 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 206010040844 Skin exfoliation Diseases 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000035618 desquamation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/03—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements self-supporting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a sample cup comprising: the cup comprises a cup body and a partition body, wherein the partition body is arranged in the cup body and divides the cup body into a first cavity and a second cavity; the partition body comprises a filter screen and an easy-to-puncture part, and the filter screen and the easy-to-puncture part are positioned on the same plane or different planes; when the filter screen and the easy-to-pierce part are positioned on the same plane, the filter screen extends on the outer side of the easy-to-pierce part; when the filter screen and the easy-to-pierce part are on different planes, the filter screen can be convexly arranged on the easy-to-pierce part or concavely arranged on the easy-to-pierce part. The invention provides a cell pretreatment method, which uses the sample cup to carry out cell pretreatment. The invention also provides a sample slide device which comprises the sample cup. Through adopting the insulator for the preservation liquid bottle has the impurity to the cell sample that drops and carries out filterable function, through set up easy puncture portion on the insulator, can not pour out the cell sap and can carry out the puncture sample, thereby prevent the secondary pollution in the cell sap transportation process.
Description
Technical Field
The invention relates to the technical field of biological inspection, in particular to a sample cup, a cell pretreatment method and a sample slide device.
Background
At present, the application range of medical inspection technology in disease screening and detection is wider and wider, and the processing before inspection is important except for the requirement on the accuracy of instruments. The screening of liquid-based cells includes: sampling by a cell brush, storing a sample, pretreating the sample, preparing and dyeing a slide, and scanning microscopic examination. Since cells collected by a cytobrush often contain impurities such as mucus, large cell aggregates, etc., the prior art methods of slide preparation often require pretreatment of the sample. The most common method is to transfer the sample from a preservation liquid cup to a centrifugal device, add the gradient centrifugate and achieve the purpose of separating impurities through multiple centrifugations. This operation is time consuming and laborious and increases the risk of contamination. If the sample is not pretreated, the prepared smear has dense impurities and uneven cell distribution, which is not beneficial to observing the cell morphology.
Through the retrieval, patent document CN209609670U discloses a body and filter equipment, filter equipment is used for filtering the foreign matter, filter equipment sets up in the inside of body to divide into first holding chamber and second holding chamber with the inside of body, during the sample, first holding intracavity is arranged in to the cervical brush, the second holding intracavity is arranged in to the straw, the filter screen blocks foreign matters such as mucus or leucorrhea in first holding chamber, the cell passes in the filter screen gets into in the second holding chamber, the straw is taken a sample in the second holding intracavity. However, the prior art has the disadvantages that the filter screen is arranged at one side of the bottle body, and the cells have higher specific gravity and can settle at one side of the bottom of the bottle body, so that a considerable number of cells cannot be taken during sampling, and the sampling representativeness is reduced, and even the missed detection is caused. Patent document CN205562242U discloses a cup body and a cup cover or a cup plug matched with the cup body, wherein a filter device is arranged in the cup body, which is disadvantageous in that the design of sleeve type is disadvantageous, the sleeve needs to be lifted up to take out a cytobrush during sampling, and operation steps are increased, and the cup is especially not favorable for full-automatic sample pretreatment. And cross contamination is easily caused in the process of lifting the sleeve, and the accuracy of a detection result is influenced.
Disclosure of Invention
In view of the drawbacks of the prior art, it is an object of the present invention to provide a specimen cup, a cell pretreatment method, and a specimen slide device, in which a partition is used for the cup body, so that a specimen can be filtered and sampled without pouring out a liquid and taking out a cell brush.
According to the present invention there is provided a sample cup comprising: the cup comprises a cup body and a partition body, wherein the partition body is arranged in the cup body and divides the cup body into a first cavity and a second cavity;
the partition body comprises a filter screen and an easy-to-pierce part, and the filter screen and the easy-to-pierce part are positioned on the same plane or different planes;
when the filter screen and the easy-to-puncture part are positioned on the same plane, the filter screen extends on the easy-to-puncture part;
when the filter screen and the easy-to-puncture part are positioned on different planes, the filter screen can be convexly arranged on the easy-to-puncture part or concavely arranged on the easy-to-puncture part;
before sampling, the easy-to-puncture part is in an integral state, and the effect of sealing a sampling port is achieved;
the side wall of the sampling needle is pressed and sealed when the easy puncture part is punctured by the sampling needle.
Preferably, a cyclic structure is also included; the filter screen is fixedly connected with the cup body through an annular structure; the cyclic structure is formed in any one of the following forms:
-a caulking groove snap form;
-widening the screen casing;
the height of the ring structure is greater than a set threshold.
Preferably, the space between the partition body and the cup bottom of the sample cup forms a second cavity, the space between the partition body and the cup cover of the sample cup forms a first cavity, the first cavity is used for placing the cell brush, and the second cavity is used for storing cell sap to be sampled.
Preferably, when the cytobrush is placed on the spacer, the portion of the cytobrush with the sample is located below the liquid level.
Preferably, the separator is located at the bottom of the first cavity, and the cells in the first cavity are dispersed in the preservation solution and can be concentrated in the second cavity through the filter screen.
Preferably, when the easy-to-puncture part is positioned at the geometric center of the separation body, the bottom of the second cavity is in a conical structure, and the conical structure can enrich settled cells.
Preferably, when the easy-to-puncture part is located on one side of the partition body, the bottom of the second cavity is of a wedge-shaped structure, and the wedge-shaped structure can enlarge the filtering area of the cell sampling liquid.
Preferably, the inner wall of the second cavity is coated with a buffer solution, so that the cell sampling solution does not remain on the inner wall of the cavity.
According to the cell pretreatment method provided by the invention, the sample cup is used for cell pretreatment.
According to a sample preparation device, the sample preparation device comprises the sample cup.
Compared with the prior art, the invention has the following beneficial effects:
1. through adopting the insulator, make the preservation liquid bottle have the function of filtering the impurity of desquamation cell sample. Avoid pouring out liquid and filter, reduced the risk that the cell sample is polluted by external environment in the bottle.
2. Through set up easy puncture portion on the insulator, can not pour out the cell sap and can carry out the puncture sample to prevent the secondary pollution in the cell sap transportation process.
3. Through setting up and placing the cytobrush in first cavity, can be under the condition of not taking out the cytobrush, can carry out sample preliminary treatment, be convenient for realize the full automatization sample of machine.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic diagram of the overall structure of a sample cup:
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.
As shown in fig. 1, the specimen preservation liquid bottle provided by the present invention includes a cup body 1201, an easy-to-pierce portion 1202, an indent 1203, a filter net 1204, a first cavity 1205, a second cavity 1206, and a spacer 1207, wherein the cup body 1201 has the spacer 1207 therein; septum 1207 comprises a filter screen 1204 and a piercing-susceptible portion 1202 which is easily pierced by a sampling needle, wherein filter screen 1204 and piercing-susceptible portion 1202 are in the same plane, and filter screen 1204 extends outside piercing-susceptible portion 1202; a spacer 1207 divides cup 1201 into a first chamber 1205 and a second chamber 1206.
Easy portion of puncturing is impaled by the sampling needle to have certain elastic rubber, when the sampling needle puncture was gone into, the sampling needle was still closely extruded all around, and the sample finishes taking out the back, and the hole that is punctured will be extruded again and seal, has reliable sealed effect, has guaranteed can not get into each other and has polluted.
Further, a second cavity 1206 is formed between the partition 1207 and the bottom of the cup, and the partition 1207 is located at the bottom of the first cavity 1205; the first chamber 1205 is used for placing a cell brush, and the second chamber 1206 is used for storing cell fluid to be sampled.
The cup body 1201 is provided with an embedding groove 1203 clamped with the outer periphery of the spacer 1207 and used for fixing the spacer 1207 and ensuring that the spacer 1207 does not deviate when stressed; when the cell brush is placed on the partition 1207, at least part of the cell brush is positioned below the liquid level, and the cells are dissolved and dispersed in the preservation solution and can be concentrated into the second cavity only through the filter screen; when the sampling needle pierces the easy-to-pierce portion 1202, the sampling needle enters the second cavity 1206 from the first cavity 1205 to aspirate the settled cells; when the easy-to-puncture part 1202 is located in the center of the partition 1207, the bottom of the second cavity 1206 is in a conical structure, and the conical structure is used for enriching settled cells and facilitating the suction of a sampling needle; the easy-to-pierce portion 1202 has flexibility, and ensures that the sampling needle extrudes and wraps the sampling needle to be sealed when piercing the sampling needle, thereby ensuring that impurities cannot enter the second cavity 1206 from the hole.
The invention also provides a sample slide device which comprises the sample cup.
Furthermore, the invention also provides a cell pretreatment method, which uses the sample cup to carry out pretreatment of cell sap.
The following are the present inventionPreferred embodiment(s) of the inventionAs will be further explained.
Can scribble the buffer solution at the cavity inner wall of second cavity 1206, can make cell sampling liquid not remain on the cavity inner wall.
The cross section of the conical structure at the bottom of the second cavity 1206 forms an angle of 60 degrees with the bottom of the liquid bottle body 1204, which is the best scheme.
The mesh size of the screen 1204 is 20 mesh to 100 mesh, preferably 60 mesh.
The following are the present inventionVariation exampleAs will be further explained.
The filter screen 1204 and the frangible portion 1202 may be in different planes, and the frangible portion 1202 may be convex or concave.
The material of the easy-to-pierce portion 1202 may be a coated aluminum foil.
In the invention, the filter screen 1204 is fastened and connected with the cup body 1201, and can adopt a caulking groove clamping connection mode; the outer frame of the filter screen can also be widened to form a ring structure with a certain height, and the ring structure is pressed and contacted with the cup body 1201 to achieve the purpose of fixing. Wherein, the height of the annular structure is larger than a set threshold value.
The working principle is as follows:
medical staff samples the back and places the cell brush in the separate body 1207 of sample cup, and through shaking the solution, the scattered subsides the cell and passes through filter screen 1204 and enter second cavity 1206, and the cell enrichment is gone into to the bottom structure of second cavity 1206. During sampling, the sampling needle pierces the easy-to-pierce portion 1202, enters the second cavity 1206 from the first cavity 1205, sucks a sufficient amount of cells from the bottom, and transfers the slide.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (10)
1. A specimen cup, comprising: the cup body (1201) and a partition body (1207), wherein the partition body (1207) is arranged in the cup body (1201), and the partition body (1207) divides the cup body (1201) into a first cavity (1205) and a second cavity (1206);
the partition body (1207) comprises a filter screen (1204) and a easily-punctured part (1202), and the filter screen (1204) and the easily-punctured part (1202) are positioned on the same plane or different planes;
when the filter screen (1204) and the easy-to-pierce part (1202) are in the same plane, the filter screen (1204) extends out of the easy-to-pierce part (1202);
when the filter screen (1204) and the easy-to-pierce part (1202) are in different planes, the filter screen (1204) can be arranged on the easy-to-pierce part (1202) in a protruding mode or arranged on the easy-to-pierce part (1202) in a recessed mode;
before sampling, the easy-to-puncture part (1202) is in an integral state and plays a role in sealing a sampling port;
the side wall of the sampling needle is pressed and sealed when the sampling needle pierces the easy puncture part (1202).
2. The specimen cup of claim 1, further comprising a ring-like structure;
the filter screen (1204) is fixedly connected with the cup body (1201) through an annular structure;
the ring structure is formed by adopting any one of the following forms:
-a caulking groove snap form;
-widening the screen casing;
the height of the ring-shaped structure is greater than a set threshold.
3. The sample cup as claimed in claim 1, wherein the space between the spacer (1207) and the bottom of the sample cup forms a second cavity (1206), the space between the spacer (1207) and the lid of the sample cup forms a first cavity (1205), the first cavity (1205) is used for placing a cell brush, and the second cavity (1206) is used for storing the cell fluid to be sampled.
4. A cuvette according to claim 3, characterized in that the part of the cytobrush with the sample is located below the liquid surface when the cytobrush is placed on the spacer (1207).
5. A cuvette according to claim 3, characterized in that the spacer (1207) is located at the bottom of the first cavity (1205), and the cells in the first cavity (1205) are dispersed in the preservation solution and can be collected in the second cavity (1206) through the sieve.
6. A sample cup according to claim 1, characterized in that the bottom of the second cavity (1206) is in a conical configuration capable of enriching settled cells when the pierceable portion (1202) is located in the geometric center of the spacer (1207).
7. A sample cup according to claim 1, characterized in that when the pierceable portion (1202) is located at one side of the spacer (1207), the bottom of the second cavity (1206) has a wedge-shaped configuration, which increases the filtration area of the cell sample fluid.
8. The specimen cup according to claim 1, wherein the inner wall of the second chamber (1206) is coated with a buffer solution, so that the cell sample solution does not remain on the inner wall of the chamber.
9. A method for pretreating cells, comprising using the specimen cup according to any one of claims 1 to 8.
10. A specimen-producing device comprising the specimen cup according to any one of claims 1 to 8.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202011074173.XA CN112263861A (en) | 2020-10-09 | 2020-10-09 | Sample cup, cell pretreatment method and sample slide device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202011074173.XA CN112263861A (en) | 2020-10-09 | 2020-10-09 | Sample cup, cell pretreatment method and sample slide device |
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CN112263861A true CN112263861A (en) | 2021-01-26 |
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CN202011074173.XA Pending CN112263861A (en) | 2020-10-09 | 2020-10-09 | Sample cup, cell pretreatment method and sample slide device |
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CN103616267A (en) * | 2013-11-13 | 2014-03-05 | 厦门宝太生物科技有限公司 | Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear |
CN105713828A (en) * | 2016-01-31 | 2016-06-29 | 广州三瑞医疗器械有限公司 | Cell preserving fluid bottle and using method thereof |
CN205562242U (en) * | 2016-04-15 | 2016-09-07 | 四川迈克生物医疗电子有限公司 | Sample is preserved cup and is adopted sample film -making device of this save cup |
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WO2020035468A1 (en) * | 2018-08-13 | 2020-02-20 | Accroma Labtec Ag | Filter apparatus for use in an automated system for the preparation of a sample for a chemical or composition analysis |
CN210493606U (en) * | 2019-06-05 | 2020-05-12 | 山东华宇工学院 | Tea making cup |
CN210811079U (en) * | 2019-07-15 | 2020-06-23 | 石家庄市第五医院 | Special built-in serum cup for testing blood collection tube |
US20200231920A1 (en) * | 2017-02-20 | 2020-07-23 | Satake Corporation | Microorganism sampling device |
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2020
- 2020-10-09 CN CN202011074173.XA patent/CN112263861A/en active Pending
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US6306350B1 (en) * | 1999-05-19 | 2001-10-23 | Itt Manufacturing Enterprises, Inc. | Water sampling method and apparatus with analyte integration |
JP2008139106A (en) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Marrow puncture liquid housing appliance and marrow puncture liquid filtering method |
CN102252865A (en) * | 2011-06-14 | 2011-11-23 | 邱清芳 | Totally closed device for sampling and treating excrement for inspection |
CN103616267A (en) * | 2013-11-13 | 2014-03-05 | 厦门宝太生物科技有限公司 | Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear |
CN106474813A (en) * | 2015-08-31 | 2017-03-08 | 中国石油化工股份有限公司 | A kind of filtration liquor separator |
CN105713828A (en) * | 2016-01-31 | 2016-06-29 | 广州三瑞医疗器械有限公司 | Cell preserving fluid bottle and using method thereof |
CN205562242U (en) * | 2016-04-15 | 2016-09-07 | 四川迈克生物医疗电子有限公司 | Sample is preserved cup and is adopted sample film -making device of this save cup |
US20200231920A1 (en) * | 2017-02-20 | 2020-07-23 | Satake Corporation | Microorganism sampling device |
WO2020035468A1 (en) * | 2018-08-13 | 2020-02-20 | Accroma Labtec Ag | Filter apparatus for use in an automated system for the preparation of a sample for a chemical or composition analysis |
CN210493606U (en) * | 2019-06-05 | 2020-05-12 | 山东华宇工学院 | Tea making cup |
CN210811079U (en) * | 2019-07-15 | 2020-06-23 | 石家庄市第五医院 | Special built-in serum cup for testing blood collection tube |
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Application publication date: 20210126 |
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