CN112251347B - Biochemical culture apparatus for environmental detection - Google Patents

Biochemical culture apparatus for environmental detection Download PDF

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Publication number
CN112251347B
CN112251347B CN202011060414.5A CN202011060414A CN112251347B CN 112251347 B CN112251347 B CN 112251347B CN 202011060414 A CN202011060414 A CN 202011060414A CN 112251347 B CN112251347 B CN 112251347B
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temperature
culture
cultivation
incubator
low
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CN112251347A (en
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杨小萍
黄福彬
李秀虹
邱加明
沈楠杰
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Fujian Zhongkai Detection Technology Co ltd
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Fujian Zhongkai Detection Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25—Greenhouse technology, e.g. cooling systems therefor

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a biochemical culture device for environment detection, in particular to the technical field of environment detection equipment. The device comprises an incubator, supporting legs, a heat insulation plate, a high-temperature culture area, a low-temperature culture area, a culture rotating basket, a first rotating shaft, a second rotating shaft, a gear, an annular gear ring, a first bevel gear, a second bevel gear, a driving motor, a dehumidifying fan, a hot air conditioner, a cold air conditioner and a drying mechanism, wherein more than two placing plates are arranged in the culture rotating basket along the vertical direction, the tops of the high-temperature culture area and the low-temperature culture area are respectively provided with a dehumidifying port, one side wall of the high-temperature culture area is provided with a hot air equalizing plate, one side wall of the low-temperature culture area is provided with a cold air equalizing plate, the hot air equalizing plate is connected with the hot air conditioner through a pipeline, the cold air equalizing plate is connected with the cold air conditioner through a pipeline, and the drying mechanism is arranged below the culture rotating basket. The uniformity of temperature distribution in the incubator is realized, and the service life of the incubator is prolonged.

Description

Biochemical culture apparatus for environmental detection
Technical Field
The invention relates to the technical field of environment detection equipment, in particular to a biochemical culture device for environment detection.
Background
The biochemical incubator is widely suitable for environmental protection, epidemic prevention, drug inspection, agricultural and livestock, aquatic product and other researches, universities and production departments, and is special constant temperature equipment for water body analysis and BOD measurement, and culture, preservation, plant cultivation and breeding experiments of bacteria, mold and microorganisms.
The existing biochemical incubator has the defects of uneven temperature distribution, uneven heating of the culture and influence on the detection result. In addition, the existing biochemical incubator cannot realize drying and moisture removal in the incubator after use, and the existence of moisture easily damages devices and influences the service life of the biochemical incubator.
Disclosure of Invention
(1) Technical problem to be solved
The invention provides a biochemical culture device for environment detection, which overcomes the defects that the temperature distribution of the existing biochemical incubator is not uniform, so that the culture is heated unevenly, the detection result is influenced, the existing biochemical incubator cannot realize drying and moisture removal in the incubator after being used, the devices are easily damaged by the moisture, and the service life of the biochemical incubator is influenced.
(2) Technical scheme
In order to solve the technical problems, the invention provides a biochemical culture device for environmental detection, which comprises an incubator, support legs, a heat insulation plate, a high-temperature culture area, a low-temperature culture area, a culture rotating basket, a first rotating shaft, a second rotating shaft, a gear, an annular gear ring, a first bevel gear, a second bevel gear, a driving motor, a moisture exhaust fan, a hot air conditioner, a cold air conditioner and a drying mechanism, wherein the support legs are arranged at the bottom of the incubator, the incubator is divided into the high-temperature culture area and the low-temperature culture area through the heat insulation plate, the first rotating shaft is vertically and downwards rotatably arranged on the inner walls of the tops of the high-temperature culture area and the low-temperature culture area, the lower end of the first rotating shaft is connected with the culture rotating basket, the second rotating shaft is rotatably arranged on one side of the culture rotating basket, and the gear is fixedly arranged in the middle of the second rotating shaft, the annular gear ring is fixedly installed in the middle of the outer side of the culture rotating basket, the annular gear ring is meshed with the gear, the upper portion of the second rotating shaft penetrates through the top of the culture box and is rotatably connected with the top of the culture box, the first bevel gear is fixedly connected to the upper end of the second rotating shaft, the second bevel gear is fixedly connected to an output shaft of the driving motor, the first bevel gear is meshed with the second bevel gear, more than two placing plates are arranged in the culture rotating basket along the vertical direction, moisture discharging ports are formed in the top of the high-temperature culture area and the top of the low-temperature culture area, moisture discharging fans are installed in the moisture discharging ports, a hot air equalizing plate is arranged on one side wall of the high-temperature culture area, a cold air equalizing plate is arranged on one side wall of the low-temperature culture area and is connected with the hot air conditioner through a pipeline, and the cold air equalizing plate is connected with the cold air conditioner through a pipeline, a drying mechanism is arranged below the cultivation rotary basket, a high-temperature box door is arranged between one side of the incubator and the heat insulation plate, and a low-temperature box door is arranged between the other side of the incubator and the heat insulation plate.
Preferably, cultivate the front of changeing the basket and be open state, cultivate the interior both sides inner wall of changeing the basket and be equipped with more than two from last to being used for the joint more than two the draw-in groove of placing the board.
Preferably, more than two hot air outlets are uniformly distributed on the hot air equalizing plate along the vertical direction, and more than two cold air outlets are uniformly distributed on the cold air equalizing plate along the vertical direction.
Preferably, the high temperature chamber door is hinged on one side of the incubator by a hinge member, and the low temperature chamber door is hinged on the other side of the incubator by a hinge member.
Preferably, sealing strips are arranged at the contact positions of the high-temperature box door and the low-temperature box door with the incubator and the heat insulation plate.
Preferably, the drying mechanism comprises an exhaust main pipe, exhaust branch pipes, a drying box, an air heater and a fan, the exhaust main pipe is arranged below the cultivation rotary basket, the top of the exhaust main pipe is communicated with more than two exhaust branch pipes, the bottom of the exhaust main pipe is connected with one end of the drying box through a pipeline, the other end of the drying box is connected with one end of the air heater through a pipeline, and the other end of the air heater is connected with the fan through a pipeline.
(3) Advantageous effects
The invention provides a biochemical culture device for environment detection, which overcomes the defects that the temperature distribution of the existing biochemical incubator is not uniform, so that the culture is heated unevenly, the detection result is influenced, the existing biochemical incubator cannot realize drying and moisture removal in the incubator after being used, the devices are easily damaged by the moisture, and the service life of the biochemical incubator is influenced. Compared with the prior art, the invention has the following beneficial effects:
1. through cultivateing both sides inner wall in the commentaries on classics basket from last to being equipped with the draw-in groove that more than two are used for the joint to place the board down, can be convenient for realize through setting up like this that every is placed the adjustment of height between the board in order to adapt to the cultivation of different volume, not co-altitude culture.
2. More than two hot air outlets are uniformly distributed on the hot air equalizing plate along the vertical direction, and more than two cold air outlets are uniformly distributed on the cold air equalizing plate along the vertical direction, so that hot air or cold air is uniformly discharged into a high-temperature culture area or a low-temperature culture area, and the uniformity of temperature distribution is realized.
3. The rotation of the first bevel gear is driven by the driving motor and then the rotation of the second bevel gear is driven by meshing, so that the second rotating shaft rotates, the rotation of the culture rotating basket is realized, and the uniform heating (cooling) of the culture is ensured.
4. The high-temperature culture area and the low-temperature culture area are separated by arranging the heat insulation plate, and the sealing performance is ensured.
5. The culture at different culture temperatures can be cultured at one time by arranging the high-temperature culture area and the low-temperature culture area in the incubator.
6. Set up the high temperature chamber door through the high temperature cultivation district, the low temperature cultivation district sets up the low temperature chamber door, can open or close corresponding chamber door pertinence, avoids two temperature in the district to receive the interference.
7. Realized the drying process to incubator inside through drying mechanism to discharge the moisture of drying process through the hydrofuge fan fast, guaranteed the drying in the incubator, the life of extension incubator.
Drawings
Fig. 1 is a schematic structural view of the high-temperature box door and the low-temperature box door of the invention when opened.
FIG. 2 is a schematic structural view of the high temperature chamber door and the low temperature chamber door of the present invention.
Fig. 3 is a schematic structural view of the hot air equalizing plate and the cold air equalizing plate of the present invention.
The reference signs are: 1-incubator, 2-supporting legs, 3-heat insulation plate, 4-high temperature culture area, 5-low temperature culture area, 6-culture rotating basket, 7-first rotating shaft, 8-second rotating shaft, 9-gear, 10-annular gear ring, 11-first bevel gear, 12-second bevel gear, 13-driving motor, 14-clamping groove, 15-placing plate, 16-moisture discharging port, 17-moisture discharging fan, 18-hot air equalizing plate, 181-hot air outlet, 19-cold air equalizing plate, 191-cold air outlet, 20-hot air conditioner, 21-cold air conditioner, 22-high temperature chamber door, 23-low temperature chamber door, 24-sealing strip, 25-drying mechanism, 251-exhaust main pipe, 252-branch pipe, 253-drying box, 254-air heater, 255-blower.
Detailed Description
The invention is further described with reference to the accompanying drawings and examples.
As shown in fig. 1 to 3, the biochemical culture apparatus for environmental testing according to the present invention comprises an incubator 1, support legs 2, a heat insulation plate 3, a high temperature culture region 4, a low temperature culture region 5, a culture basket 6, a first rotation shaft 7, a second rotation shaft 8, a gear 9, an annular gear ring 10, a first bevel gear 11, a second bevel gear 12, a driving motor 13, a dehumidifying fan 17, a hot air conditioner 20, a cold air conditioner 21, and a drying mechanism 25, wherein the support legs 2 are disposed at the bottom of the incubator 1, the incubator 1 is divided into the high temperature culture region 4 and the low temperature culture region 5 by the heat insulation plate 3, the first rotation shaft 7 is vertically and downwardly rotatably mounted on inner walls of the tops of the high temperature culture region 4 and the low temperature culture region 5, the culture basket 6 is connected to the lower end of the first rotation shaft 7, the second rotation shaft 8 is rotatably mounted on one side of the culture basket 6, the gear 9 is fixedly mounted in the middle of the second rotating shaft 8, the annular gear ring 10 is fixedly mounted in the middle of the outer side of the cultivation rotating basket 6, the annular gear ring 10 is meshed with the gear 9, the upper portion of the second rotating shaft 8 penetrates through the top of the cultivation box 1 and is rotatably connected with the top of the cultivation box 1, the first bevel gear 11 is fixedly connected to the upper end of the second rotating shaft 8, the second bevel gear 12 is fixedly connected to an output shaft of the driving motor 13, the first bevel gear 11 is meshed with the second bevel gear 12, more than two placing plates 15 are vertically arranged in the cultivation rotating basket 6, moisture exhausting ports 16 are respectively arranged at the tops of the high-temperature cultivation region 4 and the low-temperature cultivation region 5, the moisture exhausting fan 17 is mounted in the moisture exhausting ports 16, a hot air equalizing plate 18 is arranged on one side wall of the high-temperature cultivation region 4, and a cold air equalizing plate 19 is arranged on one side wall of the low-temperature cultivation region 5, the hot air homogenizing plate 18 is connected with the hot air conditioner 20 through a pipeline, the cold air homogenizing plate 19 is connected with the cold air conditioner 21 through a pipeline, a drying mechanism 25 is arranged below the cultivation rotary basket 6, a high-temperature box door 22 is arranged between one side of the incubator 1 and the heat insulation plate 3, and a low-temperature box door 23 is arranged between the other side of the incubator 1 and the heat insulation plate 3.
The front surface of the cultivation rotary basket 6 is in an open state, more than two clamping grooves 14 for clamping the placing plate 15 are arranged on the inner walls of the two sides in the cultivation rotary basket 6 from top to bottom, more than two hot air outlets 181 are uniformly distributed on the hot air equalizing plate 18 along the vertical direction, more than two cold air outlets 191 are uniformly distributed on the cold air equalizing plate 19 along the vertical direction, the high temperature box door 22 is hinged on one side of the incubator 1 through a hinge, the low temperature box door 23 is hinged on the other side of the incubator 1 through a hinge, sealing strips 24 are arranged at the positions where the high temperature box door 22 and the low temperature box door 23 are contacted with the incubator 1 and the heat insulation plate 3, the drying mechanism 25 comprises an exhaust main pipe 251, an exhaust branch pipe 252, a drying box 253, an air heater 254 and a fan 255, the exhaust main pipe 251 is arranged below the cultivation rotary basket 6, the top of the main exhaust pipe 251 is communicated with more than two branch exhaust pipes 252, the bottom of the main exhaust pipe 251 is connected with one end of the drying box 253 through a pipeline, the other end of the drying box 253 is connected with one end of the air heater 254 through a pipeline, and the other end of the air heater 254 is connected with the fan 255 through a pipeline.
In actual work, according to the temperature that the culture needs to be cultivated on placing plate 15 of culture rotating basket 6 in high temperature culture area 4 or low temperature culture area 5 with the culture, cultivate and be equipped with more than two and be used for the joint from last to down to the inner wall of both sides in rotating basket 6 place the draw-in groove 14 of plate 15, can be convenient for realize through setting up like this that every is placed the adjustment of height between the plate 15 in order to adapt to the cultivation of not co-altitude culture of different volumes. After the culture medium is placed, the corresponding high-temperature box door 22 or low-temperature box door 23 is closed, the driving motor 13 is started to drive the first bevel gear 11 to rotate so as to drive the second bevel gear 12 to rotate in a meshed mode, the second rotating shaft 8 rotates, the culture rotating basket 6 rotates along with the second bevel gear, the corresponding hot air conditioner 20 or hot air conditioner 21 is started to discharge hot air or cold air into the hot air equalizing plate 18 or cold air equalizing plate 19 correspondingly, more than two hot air outlets 181 are uniformly distributed in the vertical direction on the hot air equalizing plate 18, more than two cold air outlets 191 are uniformly distributed in the cold air equalizing plate 19 in the vertical direction, hot air or cold air is uniformly discharged into the high-temperature culture area 4 or the low-temperature culture area 5, and the uniformity of temperature distribution is achieved. Meanwhile, the rotation of the culture rotating basket 6 is matched, so that the uniform heating (cooling) of the culture is ensured. The high-temperature culture area 4 and the low-temperature culture area 5 are separated by arranging the heat insulation plate 3, and the sealing performance is ensured. The culture at different culture temperatures can be cultured once by setting the high-temperature culture zone 4 and the low-temperature culture zone 5 in the incubator 1, the high-temperature culture zone 4 is provided with the high-temperature box door 22, the low-temperature culture zone 5 is provided with the low-temperature box door 23, the corresponding box door can be opened or closed pertinently, and the temperature in the two zones is prevented from being interfered. The provision of the sealing strip 24 improves the sealing when the door is closed. After the incubator 1 is used, the wind generated by the fan 255 can be started to enter the air heater 254 and be converted into hot wind, then the drying is realized through the drying box 253, then the drying is carried out, the drying is carried out on the exhaust main pipe 251, the exhaust is uniformly carried out on the exhaust branch pipe 252, the drying treatment on the inside of the incubator 1 is realized, meanwhile, the dehumidifying fan 17 can be opened, the moisture in the drying process can be rapidly exhausted through the dehumidifying fan 17, the drying in the incubator is ensured, and the service life of the incubator is prolonged.
The above-described embodiments only express the preferred embodiments of the present invention, and the description thereof is more specific and detailed, but the present invention is not limited to these embodiments, and it should be noted that it is obvious to those skilled in the art. Any modification falling within the scope of protection of the present invention without departing from the gist of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (4)

1. The biochemical culture device for environment detection is characterized by comprising an incubator (1), supporting legs (2), an insulating board (3), a high-temperature culture area (4), a low-temperature culture area (5), a culture rotating basket (6), a first rotating shaft (7), a second rotating shaft (8), a gear (9), an annular gear ring (10), a first bevel gear (11), a second bevel gear (12), a driving motor (13), a dehumidifying fan (17), a hot air conditioner (20), a cold air conditioner (21) and a drying mechanism (25), wherein the supporting legs (2) are arranged at the bottom of the incubator (1), the high-temperature culture area (4) and the low-temperature culture area (5) are separated from the inside of the incubator (1) through the insulating board (3), the first rotating shaft (7) is vertically and downwardly rotatably installed on the inner walls of the tops of the high-temperature culture area (4) and the low-temperature culture area (5), the lower end of the first rotating shaft (7) is connected with the cultivation rotating basket (6), one side of the cultivation rotating basket (6) is rotatably provided with the second rotating shaft (8), the middle part of the second rotating shaft (8) is fixedly provided with the gear (9), the middle part of the outer side of the cultivation rotating basket (6) is fixedly provided with the annular gear ring (10), the annular gear ring (10) is meshed with the gear (9), the upper part of the second rotating shaft (8) penetrates through the top of the cultivation box (1) and is rotatably connected with the top of the cultivation box (1), the upper end of the second rotating shaft (8) is fixedly connected with the first bevel gear (11), the output shaft of the driving motor (13) is fixedly connected with the second bevel gear (12), the first bevel gear (11) is meshed with the second bevel gear (12), more than two placing plates (15) are arranged in the cultivation rotating basket (6) along the vertical direction, the high-temperature cultivation area (4) and the top of the low-temperature cultivation area (5) are provided with a moisture discharging port (16), a moisture discharging fan (17) is installed in the moisture discharging port (16), a hot air equalizing plate (18) is arranged on one side wall of the high-temperature cultivation area (4), a cold air equalizing plate (19) is arranged on one side wall of the low-temperature cultivation area (5), more than two hot air outlets (181) are uniformly distributed on the hot air equalizing plate (18) along the vertical direction, more than two cold air outlets (191) are uniformly distributed on the cold air equalizing plate (19) along the vertical direction, the hot air equalizing plate (18) is connected with a hot air conditioner (20) through a pipeline, the cold air equalizing plate (19) is connected with the cold air conditioner (21) through a pipeline, a drying mechanism (25) is arranged below the cultivation rotary basket (6), and the drying mechanism (25) comprises an exhaust main pipe (251), The air exhaust branch pipe (252), the drying box (253), the air heater (254) and the fan (255) are arranged, the air exhaust main pipe (251) is arranged below the cultivation rotary basket (6), the top of the air exhaust main pipe (251) is communicated with more than two air exhaust branch pipes (252), the bottom of the air exhaust main pipe (251) is connected with one end of the drying box (253) through a pipeline, the other end of the drying box (253) is connected with one end of the air heater (254) through a pipeline, the other end of the air heater (254) is connected with the fan (255) through a pipeline, a high-temperature box door (22) is arranged between one side of the cultivation box (1) and the heat insulation plate (3), and a low-temperature box door (23) is arranged between the other side of the cultivation box (1) and the heat insulation plate (3).
2. The biochemical culture apparatus for environmental monitoring according to claim 1, wherein the front surface of the culture basket (6) is open, and two or more slots (14) for clamping the placement plate (15) are formed in the inner walls of the two sides of the culture basket (6) from top to bottom.
3. The biochemical culture device for environmental detection according to claim 1, wherein the high temperature chamber door (22) is hinged on one side of the incubator (1) by a hinge member, and the low temperature chamber door (23) is hinged on the other side of the incubator (1) by a hinge member.
4. The biochemical culture apparatus for environmental monitoring according to claim 1, wherein a sealing strip (24) is provided at a position where the high temperature chamber door (22) and the low temperature chamber door (23) contact the incubator (1) and the heat insulating plate (3).
CN202011060414.5A 2020-09-30 2020-09-30 Biochemical culture apparatus for environmental detection Active CN112251347B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7776584B2 (en) * 2003-08-01 2010-08-17 Genetix Limited Animal cell colony picking apparatus and method
CN206014889U (en) * 2016-08-31 2017-03-15 云南坤发环境科技有限公司 A kind of biochemical cultivation case for biological detection
CN207109015U (en) * 2017-07-14 2018-03-16 华夏安健检测评价技术服务有限公司 A kind of public health detection CO2gas incubator
CN108823096A (en) * 2018-08-14 2018-11-16 南京唯诺尔科技项目管理有限公司 A kind of biotechnology cell culture apparatus
CN209193978U (en) * 2018-10-24 2019-08-02 云南迪安医学检验所有限公司 A kind of microorganism fast culture detection device
CN109971640B (en) * 2019-04-13 2022-04-01 河南省中医院（河南中医药大学第二附属医院） Clinical laboratory's bacterial incubator

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