CN112239727B - Method for culturing microalgae based on artificial intelligence microalgae mixing system - Google Patents
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Abstract
The invention discloses a method for culturing microalgae based on an artificial intelligence microalgae mixing system, and relates to the technical field of microalgae culture. The method realizes the balanced management of the mixing energy by measuring the temperature and the light intensity in real time to adjust the mixing intensity. More importantly, the method can enhance the mixing efficiency of the saved energy in the optimal growth time, thereby being expected to further improve the production efficiency of the microalgae. Therefore, the invention can reduce the mixing energy consumption and ensure the high-efficiency growth of the microalgae at the same time, and has great application potential.
Description
Technical Field
The invention relates to the technical field of microalgae culture, in particular to a microalgae culture method based on artificial intelligence.
Background
As a kind of photosynthetic microorganism, the microalgae has the advantages of high propagation speed, high photosynthesis efficiency and CO2High fixation efficiency, rich nutrients such as protein, polysaccharide, polyunsaturated fatty acid and pigment, easy culture and no occupation of valuable farmland resources. Due to the advantages, the microalgae has great application potential in the aspects of food, aquatic animal feed, biological energy, sewage treatment, carbon emission reduction and the like, and is the most favorable candidate for solving the current environmental, energy and food crisis.
The high-efficiency and low-cost culture system is the key for promoting the large-scale industrial application of microalgae. At present, the culture system of microalgae mainly comprises a closed photobioreactor and an open culture system, such as an open pond. The photobioreactor is reliable in culture conditions, high in culture density and efficiency, but high in manufacturing and operating cost, so that the photobioreactor can only be applied to commercial production of high-value microalgae products such as haematococcus pluvialis, phaeodactylum tricornutum and the like at present. In contrast, open culture systems have lower manufacturing and operating costs, which are currently being applied to large scale outdoor commercial production of microalgae. But the production efficiency is low and uncontrollable, and the microalgae biomass with lower cost is difficult to produce by using the culture system.
In fact, poor mixing efficiency is the main reason for the low production efficiency of microalgae culture systems. Mixing is an important factor affecting microalgae growth, such as its ability to promote mass transfer; the switching frequency (namely the flashing effect) of the cells in the light and dark interval is improved, and the higher flashing effect can improve the photosynthesis efficiency of the microalgae; preventing cell sedimentation and local over-temperature. In addition, the mixing effect of open culture systems is spatially unbalanced. As reported by Mendoza, the oxygen mass transfer coefficients at the open cell paddle, at the gas sparge, straight and curved near the paddle were 164.50, 63.66, 0.87, 0.94h, respectively-1. Therefore, it is important to improve the productivity of the open culture system to improve the mixing intensity, particularly the mixing efficiency in the entire area of the open culture system.
In addition to mixing efficiency, temperature and light intensity are two other very important factors that affect microalgae growth. Theoretically, the growth rate of microalgae is the highest only if the optimal temperature and illumination intensity of microalgae are maintained simultaneously. However, due to the limitation of natural light-dark cycle, the optimal temperature and optimal illumination intensity of microalgae in the open pond cannot be synchronized, and the time during which the optimal temperature and optimal illumination intensity can be maintained simultaneously in one day is very limited, while the corresponding non-optimal production time is long. On the other hand, the mixing of microalgae cultivation systems is operated at a constant speed all the day, which results in the open pond wasting too much energy in a non-optimal production time.
Disclosure of Invention
The invention provides a method for culturing microalgae based on an artificial intelligence microalgae mixing system, aiming at improving the production efficiency of the microalgae culturing system and solving the problem of energy consumption of the microalgae culturing system which is wasted in non-optimal production time. The artificial intelligence mixed microalgae system adjusts the mixing intensity through the culture solution temperature and the illumination intensity (light intensity) detected in real time, so that the excessive energy is prevented from being wasted in the non-optimal production time, the saved energy consumption can be used for improving the mixing efficiency in the optimal production time, and the purposes of energy conservation and high efficiency production are achieved.
Prior to the present disclosure, in order to improve the production efficiency of the culture system, the mixing efficiency is generally improved by increasing the mixing intensity or by adding a baffle inside the culture system. Although these methods can significantly improve the production efficiency of the culture system, they all come at the expense of higher energy consumption. This is mainly because these methods do not take into account the mismatch between the temperature and the light intensity due to the natural light-dark cycle, i.e., the optimal temperature and the optimal illumination intensity of the microalgae cannot be synchronized, and the time during which the optimal temperature and the optimal illumination intensity can be maintained at the same time in one day is very limited. Thus, the above method wastes excessive energy in a non-optimal production time that is long. In contrast, the technology disclosed by the invention utilizes an artificial intelligence microalgae mixing system to culture microalgae, and the system realizes the balanced management of mixing energy by a method of measuring the temperature and the light intensity in real time to adjust the mixing intensity, so that the mixing energy is intensively utilized in the optimal growth time, and the excessive waste in the non-optimal production time is avoided.
Specifically, the technical scheme of the invention is as follows:
a method for culturing microalgae based on an artificial intelligence microalgae mixing system comprises the following steps:
(1) connecting the artificial intelligent microalgae mixing system with a mixing device of a microalgae culture system;
(2) putting a microalgae culture medium into the microalgae culture system containing the artificial intelligent mixing system, and inoculating microalgae into the culture medium;
(3) the power supply of the mixing device is switched on, and the mixing device controls the running speed of the mixing device according to the control program instruction built in the artificial intelligent mixing system so as to control the mixing intensity, thereby carrying out microalgae culture.
According to the technical scheme, the artificial intelligence microalgae mixing system comprises a controller, a thermometer and a light intensity instrument, wherein the controller is respectively connected with the thermometer and the light intensity instrument, and the thermometer and the light intensity instrument are respectively used for monitoring the temperature and the light intensity of the culture solution in real time.
According to the technical scheme, the controller is connected with the mixing device of the microalgae culture system, and the mixing strength is adjusted by measuring the temperature and the light intensity of the culture solution in real time. The controller is a controller which is programmed to control the motor to run and stop at a certain frequency, is mainly used for monitoring the processing of data and the command control of the mixing device, can read and process the monitored temperature and light intensity data, sends pulses to the driver of the stepping motor through a programmed program, and the stepping motor receives signals to drive the mixing device to run at a given speed.
According to the technical scheme, the controller is electrically connected with the light intensity instrument, the mixing device and the thermometer respectively.
According to the above technical solution, the microalgae cultivation system can be, but is not limited to: closed photobioreactors such as open raceway ponds, round ponds, flat plate photobioreactors, pipeline photobioreactors, column photobioreactors and floating photobioreactors.
According to the technical scheme, the mixing device comprises a stirring paddle, a circulating water pump, aerated air stones and a pipeline.
According to the above technical solution, the method (mixing adjustment strategy) for adjusting the mixing intensity in real time by the measured temperature T and illumination intensity L of the culture fluid in the artificial intelligence microalgae mixing system is as follows:
when the temperature T is less than or equal to 15.0 ℃, the rotating speed n of the stirring paddle motor is automatically adjusted to be more than 30rpm and less than or equal to 60 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 240rpm<n≤300rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 400 mu mol.m-2·s-1<L≤1000μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 240rpm<n≤300rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L>1000μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 300rpm<n≤360rpm；
When the temperature T is>30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is>30.0 ℃ and a light intensity L of 50 [ mu ] mol m-2·s-1<L≤200μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is>30.0 ℃ and a light intensity L of 200. mu. mol. m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is>30.0 ℃ and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 300rpm<n is less than or equal to 360 rpm. Specifically, see the following table:
note: illumination intensity L unit: μ mol. m-2·s-1Temperature, T units: DEG C, motor speed n unit: rpm.
The method (mixing adjustment strategy) for adjusting the mixing intensity in real time by the measured culture solution temperature T and the illumination intensity L of the artificial intelligent microalgae mixing system is preferably as follows:
when the temperature T is less than or equal to 15.0 ℃, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L>400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 260 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 400 mu mol.m-2·s-1<L≤1000μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 260 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L>1000μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 320 rpm;
when the temperature T is>30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is>30.0 ℃ and a light intensity L of 50 [ mu ] mol m-2·s-1<L≤200μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is>30.0 ℃ and a light intensity L of 200. mu. mol. m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is>30.0 ℃ and the illumination intensity L>400μmol·m-2·s-1In the meantime, the rotation speed n of the paddle motor is automatically adjusted to 320 rpm. Specifically, see the following table:
note: illumination intensity L unit: μ mol. m-2·s-1Temperature, T units: DEG C, motor speed n unit: rpm.
According to the technical scheme, the carbon source used for culturing the microalgae comprises organic carbon sources such as acetic acid, glucose, ethanol, sodium acetate and the like, and CO2And a bicarbonate.
According to the above technical scheme, the microalgae are Dunaliella salina (Dunaliella sp.), Chlorella vulgaris (Chlorella sp.), Lysimachia hypotenus (Euhalothece sp.), Spirulina (Spirolinia.), Microcoleus vaginalis (Microcoleus sp.), Synechocystis sp.), Isochrysis globosa (Isochrysis sp.), Verticillium parvum (Prymnesium sp.), Neochloris fulvus (Neochloris oleabunans), Nannochloropsis globuloides (Nannochloropsis), and Phaeodactylum tricornutum (Phaeodactylum tricornutum).
Compared with the traditional continuous mixed culture method, the invention has the following advantages:
the invention provides a method for culturing microalgae based on an artificial intelligence microalgae mixing system, which realizes balanced management of mixing energy by adjusting mixing intensity by measuring temperature and light intensity in real time. More importantly, the method can enhance the mixing efficiency of the saved energy in the optimal growth time, thereby being expected to further improve the production efficiency of the microalgae. Therefore, the method can be used for culturing the microalgae with energy conservation and high efficiency, can ensure the high-efficiency growth of the microalgae while reducing the mixing energy consumption, and has great application potential.
Drawings
FIG. 1 is a schematic diagram of an artificial intelligent microalgae hybrid system.
FIG. 2 is a graph showing the effect of temperature and light intensity on the growth of Spirulina in example 1.
FIG. 3 shows the intelligent open pond culture of Spirulina in example 2.
Detailed Description
The following non-limiting examples are presented to enable those of ordinary skill in the art to more fully understand the present invention and are not intended to limit the invention in any way. The following is merely an exemplary illustration of the scope of the claims of the present application and various changes and modifications of the invention of the present application may be made by those skilled in the art based on the disclosure, which should also fall within the scope of the claims of the present application.
EXAMPLE 1 Effect of temperature and light intensity on Spirulina growth
Firstly, the following culture media are prepared: 25.2g L-1NaHCO3，2.5g L-1NaNO3，0.04g L-1CaCl2·2H2O,1.0g L-1K2SO4,0.5g L-1K2HPO4,0.2g L-1MgSO4·7H2O,0.01g L-1FeSO4·7H2O,1.979g L- 1MoCl2·4H2O，3.092g L-1H3BO3，0.484g L-1NaMo4·2H2O，0.23g L-1ZnSO4·7H2O，0.183g L- 1NaVO3，0.048g L-1CoCl2·6H2O，0.2g L-1CuSO4·5H2O。
The culture medium was injected into a rectangular tubular photobioreactor of 10cm height, 5.0cm width and 20cm length, the mixing of the reaction was provided by a rocking platform set at 60rpm in a continuous mode. Then fresh spirulina FACHB-314 was inoculated into the reactor, and the final inoculum volume and inoculum density were controlled to 200ml and 0.1g L respectively-1. Finally, at 50, 200 and 400. mu. mol. m, respectively-2·s-1Under three continuous illumination intensities, the influence of temperatures of 15, 20, 25, 30, 35, 40 ℃ and the like on the growth of the spirulina is examined, and the culture time is 2 days.
As shown in FIG. 2, at low light intensity (50.0. mu. mol. m)-2·s-1) Under culture conditions, specific growth rates of spirulina were less than 1 at all temperature conditions studied. At a low temperature of<20 deg.C), high light intensity (200 and 400. mu. mol. m)-2·s-1) The specific growth rate of spirulina under the culture conditions is also less than 1. In contrast, spirulina was found to have high luminous intensity (200 and 400. mu. mol. m)-2·s-1) The specific growth rate under the culture conditions of 20-35 ℃ is greater than 1, wherein at 400 [ mu ] mol.m-2·s-1The specific growth rate under the culture condition of 25 ℃ is the maximum and is 1.83 +/-0.01 d-1. The above results indicate that spirulina grows fastest only under the condition of optimum temperature and optimum illumination intensity at the same time.
Example 2 Intelligent open pond culture of Spirulina
The 400L of the medium described in example 1 was poured into a 2.0m cultivation area2The thickness of the liquid layer after the culture medium is injected into the raceway pond is 20.0 cm. The mixing of the culture solution in the open pond is driven by a stirring paddle of the culture solution, wherein the stirring paddle is connected with a controller of the intelligent mixing system through a data line and is operated under the instruction of the controller. In addition, the controller is also respectively connected with a light intensity instrument and a thermometer and is respectively used for detecting and acquiring light intensity and temperature, wherein the measured temperature is the temperature of the culture solutionAnd (4) degree. The controller will be programmed to control the speed of rotation of the paddles according to the data detected in real time, according to the experimental results from example 1. A specific intelligent open cell mixing control strategy, under which the paddles would be adjusted and operated in real time at motor speeds of 30, 120, 200, 260, 320rpm, is shown in the table below. As a control, two additional identical open cells were set, but they were constantly operated at stirring speeds of 30rpm and 320rpm, respectively, and were designated as low-speed open cells and high-speed open cells, respectively. Finally according to 0.05g L-1The spirulina is inoculated with the spirulina FACHB-314, and the spirulina is cultured under the outdoor natural condition, the stirring paddle runs for 14 hours every day (from 5 o 'clock to 7 o' clock later), and the culture time is 9 days.
Note: illumination intensity L unit: μ mol. m-2·s-1Temperature, T units: DEG C, motor speed n unit: rpm.
As shown in FIG. 3, after 9 days of culture, the intelligent open cell obtained the same cell density as the high-speed open cell, which was 0.233. + -. 0.00g L-And 0.237. + -. 0.01g L-1And there is no significant difference (p) between them<0.05). And the cell density of the two open cells is higher than that of the low-speed open cell (0.186 +/-0.01 g L)-1，p>0.05), which indicates that enhancing the mixing efficiency can significantly improve the production efficiency. More importantly, the intelligent open pond of the invention also saves 38.6% of energy when the same culture level as the high-speed open pond is achieved, wherein the energy consumption of the intelligent open pond and the open pond is 12.18kWh and 19.85kWh respectively. In conclusion, the invention can save energy consumption and maintain higher production efficiency.
It will be apparent to those skilled in the art from this disclosure that many changes and modifications can be made, or equivalents modified, in the embodiments of the invention without departing from the scope of the invention. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention shall still fall within the protection scope of the technical solution of the present invention, unless the contents of the technical solution of the present invention are departed.
Claims (5)
1. A method for culturing microalgae based on an artificial intelligence microalgae mixing system is characterized in that the artificial intelligence microalgae mixing system can adjust the mixing intensity in real time through the measured temperature and illumination intensity of a culture solution;
the artificial intelligent microalgae mixing system comprises a microalgae culture system and a mixing device, wherein the microalgae culture system is an open raceway pond or a circular pond, and the mixing device is a stirring paddle;
the microalgae is Spirulina (Spirulina);
the method for adjusting the mixing intensity of the artificial intelligent microalgae mixing system in real time through the measured temperature and illumination intensity of the culture solution comprises the following steps:
when the temperature T is less than or equal to 15.0 ℃, the rotating speed n of the stirring paddle motor is automatically adjusted to be 30rpm < n < 60 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 240rpm<n≤300rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 400 mu mol.m-2·s-1<L≤1000μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 240rpm<n≤300rpm；
When the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L>1000μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 300rpm<n≤360rpm；
When the temperature T is>30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30rpm<n≤60rpm；
When the temperature T is>30.0 ℃ and a light intensity L of 50 [ mu ] mol m-2·s-1<L≤200μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 60rpm<n≤180rpm；
When the temperature T is>30.0 ℃ and a light intensity L of 200. mu. mol. m-2·s-1<L≤400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 180rpm<n≤240rpm；
When the temperature T is>30.0 ℃ and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 300rpm<n≤360rpm。
2. A method for culturing microalgae based on an artificial intelligence microalgae mixing system is characterized in that the artificial intelligence microalgae mixing system can adjust the mixing intensity in real time through the measured temperature and illumination intensity of a culture solution;
the artificial intelligent microalgae mixing system comprises a microalgae culture system and a mixing device, wherein the microalgae culture system is an open raceway pond or a circular pond, and the mixing device is a stirring paddle;
the microalgae is Spirulina (Spirulina);
the method for adjusting the mixing intensity of the artificial intelligent microalgae mixing system in real time through the measured temperature and illumination intensity of the culture solution comprises the following steps:
when the temperature T is less than or equal to 15.0 ℃, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is 15.0 DEG C<T is less than or equal to 20.0 ℃, and the illumination intensity L>400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and illumination is performedStrength L less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is 20.0 DEG C<T is less than or equal to 25.0 ℃, and the illumination intensity L>400μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 260 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 50 mu mol.m-2·s-1<L≤200μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 200 mu mol.m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L is 400 mu mol.m-2·s-1<L≤1000μmol·m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 260 rpm;
when the temperature T is 25.0 DEG C<T is less than or equal to 30.0 ℃, and the illumination intensity L>1000μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 320 rpm;
when the temperature T is>30.0 ℃, and the illumination intensity L is less than or equal to 50 mu mol.m-2·s-1During the process, the rotating speed n of the stirring paddle motor is automatically adjusted to 30 rpm;
when the temperature T is>30.0 ℃ and a light intensity L of 50 [ mu ] mol m-2·s-1<L≤200μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 120 rpm;
when the temperature T is>30.0 ℃ and a light intensity L of 200. mu. mol. m-2·s-1<L≤400μmol·m-2·s-1When the speed is high, the rotating speed n of the stirring paddle motor is automatically adjusted to 200 rpm;
when the temperature T is>30.0 ℃ and the illumination intensity L>400μmol·m-2·s-1In the meantime, the rotation speed n of the paddle motor is automatically adjusted to 320 rpm.
3. The method as claimed in claim 1 or 2, wherein the artificial intelligence microalgae hybrid system comprises a controller, a thermometer and a light intensity meter, wherein the controller is respectively connected with the thermometer and the light intensity meter, and the thermometer and the light intensity meter are respectively used for monitoring the temperature and the illumination intensity of the culture solution in real time.
4. The method of claim 3, wherein the controller is connected to a mixing device of the microalgae cultivation system, and the controller adjusts the mixing intensity by measuring the temperature and illumination intensity of the culture solution in real time.
5. The method according to claim 1 or 2, wherein the carbon source used for culturing the microalgae is an organic carbon source, CO2And a bicarbonate.
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