CN112229698A - Liquid-based thin-layer cell technology-based liquid-based cell full-automatic dyeing machine - Google Patents
Liquid-based thin-layer cell technology-based liquid-based cell full-automatic dyeing machine Download PDFInfo
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- CN112229698A CN112229698A CN202011240556.XA CN202011240556A CN112229698A CN 112229698 A CN112229698 A CN 112229698A CN 202011240556 A CN202011240556 A CN 202011240556A CN 112229698 A CN112229698 A CN 112229698A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 60
- 239000007788 liquid Substances 0.000 title claims abstract description 53
- 238000005516 engineering process Methods 0.000 title claims abstract description 17
- 230000007246 mechanism Effects 0.000 claims abstract description 48
- 238000010186 staining Methods 0.000 claims abstract description 27
- 239000011521 glass Substances 0.000 claims description 56
- 230000007704 transition Effects 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 230000003028 elevating effect Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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Abstract
A liquid-based thin-layer cell technology-based liquid-based cell full-automatic dyeing machine relates to the field of precision test instruments and comprises a machine base; an upper cover movably connected with one side of the base; the first cylinder and the second cylinder are fixed on one side of the base and are fixed with the inner walls of the two sides of the upper cover; a plurality of dyeing box seats are uniformly distributed on the upper surface of the machine seat by taking the center of the machine seat as a circle center; a staining box placed in the staining box seat; a vertical shaft installed in the center through hole of the machine base; the rotating mechanism is arranged in the base and is connected with the lower end of the vertical shaft, and the rotating mechanism can drive the vertical shaft to rotate around the axis of the rotating mechanism; the lifting mechanism is slidably mounted on the vertical shaft and can move up and down along the vertical shaft; a slide cassette fixed to the lifting mechanism, wherein a slide is placed in the slide cassette. The invention has the advantages of easy operation, safety, reliability, energy saving, environmental protection, low cost, high dyeing efficiency and good effect.
Description
Technical Field
The invention relates to the technical field of precision test instruments, in particular to a liquid-based cell full-automatic dyeing machine based on a liquid-based thin-layer cell technology.
Background
At present, in the aspect of cell staining, a plurality of hospitals mainly adopt a traditional manual staining mode, and the manual staining mode has many defects and shortcomings, such as long time consumption and low efficiency, and meanwhile, the staining quality has great difference according to different personal experiences, and the domestic increasing staining requirements are difficult to meet.
Disclosure of Invention
The invention provides a liquid-based cell full-automatic dyeing machine based on a liquid-based thin-layer cell technology, aiming at solving the problems of long time consumption, low efficiency, unstable quality and poor effect in the existing manual cell dyeing.
The technical scheme adopted by the invention for solving the technical problem is as follows:
the invention relates to a liquid-based thin-layer cell technology-based liquid-based cell full-automatic dyeing machine, which comprises:
a machine base;
an upper cover movably connected with one side of the base;
the first cylinder and the second cylinder are fixed on one side of the base and are fixed with the inner walls of the two sides of the upper cover;
a plurality of dyeing box seats are uniformly distributed on the upper surface of the machine seat by taking the center of the machine seat as a circle center;
a staining box placed in the staining box seat;
a dyeing liquid placed in the dyeing box;
a vertical shaft installed in the center through hole of the machine base;
the rotating mechanism is arranged in the base and is connected with the lower end of the vertical shaft, and the rotating mechanism can drive the vertical shaft to rotate around the axis of the rotating mechanism;
the lifting mechanism is slidably mounted on the vertical shaft and can move up and down along the vertical shaft;
a slide cassette secured to the lift mechanism, the slide cassette having a slide disposed therein.
The control system is respectively and electrically connected with the switch, the control display screen, the lifting mechanism, the rotating mechanism, the first cylinder and the second cylinder.
Further, the rotation mechanism includes:
the bearing seat and the motor seat are fixed in the base;
the lower end of the vertical shaft is in transition fit with an inner hole of the thrust bearing, the lower end face of the thrust bearing is in contact with the bearing seat, the upper end face of the thrust bearing is in contact with the lower end face of an inner ring of the first ball bearing, the inner hole of the first ball bearing and the inner hole of the second ball bearing are in transition fit with the outer diameter of the vertical shaft, the inner ring of the first ball bearing and the inner ring of the second ball bearing rotate together with the vertical shaft, the outer diameter of the first ball bearing and the outer diameter of the second ball bearing are fixedly connected with the bearing seat, and the inner diameter of the rotary driven gear is in movable fit with the outer diameter;
the rotary servo motor is fixed on the motor base and is electrically connected with the control system;
the inner hole of the rotary driving gear is tightly matched with an output shaft of the rotary servo motor;
the first sensor, the identification system and the second sensor are sequentially fixed on the vertical shaft from top to bottom; the first sensor, the identification system and the second sensor are electrically connected with the control system respectively.
Furthermore, a vertical shaft key groove is formed in the outer diameter of the vertical shaft, a rotating driven gear key groove is formed in an inner hole of the rotating driven gear, first flat keys are mounted on the vertical shaft key groove and the rotating driven gear key groove, half of two side faces of each first flat key are in transition fit with the vertical shaft key groove, and the other half of two side faces of each first flat key are in movable fit with the side face of the rotating driven gear key groove.
Furthermore, an output shaft key groove is formed in the outer diameter of an output shaft of the rotary servo motor, a rotary driving gear key groove is formed in an inner hole of the rotary driving gear, a second flat key is arranged on the output shaft key groove and the rotary driving gear key groove, one half of two side faces of the second flat key are in transition fit with the output shaft key groove, the other half of two side faces of the second flat key are in movable fit with the side face of the rotary driving gear key groove, torque is transmitted through the second flat key, and the second flat key plays a role in fixing the output shaft of the rotary servo motor and the rotary driving.
Further, the rotating mechanism further includes: a photoelectric encoder mounted on the vertical shaft; the photoelectric encoder is electrically connected with the control system.
Further, the lifting mechanism includes:
a lifting guide rail rack fixed on the outer wall of the upper end of the vertical shaft;
a slide glass lifting sliding frame is arranged at the upper end of the vertical shaft through a lifting driving gear;
the lifting servo motor is fixed on the slide glass lifting sliding frame and is electrically connected with the control system, an output shaft of the lifting servo motor is tightly matched with the lifting driving gear, and the lifting driving gear is meshed with the lifting guide rail rack;
a slide support fixed to the slide lifting carriage;
the slide glass box fixing rod is vertically fixed at the other end of the slide glass bracket, a dovetail groove is formed in the other end of the slide glass box fixing rod, a dovetail structure is arranged at the upper end of the slide glass box, and the slide glass box is clamped and fixed in the dovetail groove of the slide glass box fixing rod through the dovetail structure; the slide glass box is driven to move up and down or shake by the lifting servo motor.
Furthermore, the device also comprises a waste liquid tank arranged in the machine base.
The invention has the beneficial effects that:
aiming at the defects in the prior art, the invention designs a precision instrument integrating mechanical, electrical, pneumatic and program control into a whole, and specially designs a slide glass box, a lifting mechanism and a rotating mechanism, wherein a vertical shaft rotates to the corner of each dyeing tank for positioning, the slide glass bracket vibrates at high frequency, and slides stay in dyeing liquid of the dyeing box for different time, 6-10 slides coated with uniform cells are loaded into the slide glass box, the instrument is operated to automatically identify the slides and the corresponding dyeing liquid, the slides automatically enter the corresponding dyeing liquid respectively, and can be shaken to ensure uniform and stable dyeing, the dyeing time and amplitude can be adjusted and controlled, the dyeing process is fully automatic, the dyeing effect is good, and the performance is stable.
The invention overcomes the defects of unstable quality and poor effect of artificial dyeing, removes the factors of artificial operation errors, and greatly improves the dyeing efficiency and quality of the liquid-based cell reagent.
The invention realizes the automation of cell dyeing, has safe and convenient operation, uniform treatment of waste dyeing liquid, closed instrument operation and no pollution in use, and has the advantages of easy operation, safety, reliability, energy conservation, environmental protection and low cost.
The invention obviously improves the dyeing quality, improves the dyeing efficiency by more than 20 times compared with the artificial dyeing, is suitable for the cell dyeing work of large, medium and small hospitals in China, and fills the technical blank in the field in China.
Drawings
Fig. 1 is a schematic structural diagram of a liquid-based cell full-automatic staining machine based on a liquid-based thin-layer cell technology.
Fig. 2 is a schematic side view of the liquid-based cell full-automatic staining machine based on the liquid-based thin-layer cell technology.
FIG. 3 is a schematic view of the positional relationship between the lifting rail rack and the slide lifting carriage.
Fig. 4 is an internal schematic view of the elevating mechanism.
Fig. 5 is an internal schematic view of the elevating mechanism.
FIG. 6 is a schematic view showing the connection relationship between the rotary driven gear and the vertical shaft.
Fig. 7 is a sectional view showing a connection relationship between the rotary driven gear shown in fig. 6 and the vertical shaft.
In the figure, 1, a first sensor, 2, a slide box, 3, a slide, 4, a staining box, 5, a base, 6, a switch, 7, a second sensor, 8, a vertical shaft, 9, a control display screen, 10, a staining box base, 11, a lifting guide rail rack, 12, a lifting servo motor, 13, a first cylinder, 14, an upper cover, 15, a slide support, 16, a second cylinder, 17, a thrust bearing, 18, a first ball bearing, 19, a rotary driven gear, 20, a rotary servo motor, 21, a rotary driving gear, 22, a second ball bearing, 23, an identification system, 24, a slide lifting carriage, 25, a slide box fixing rod, 26, a lifting driving gear, 27, a vertical shaft key groove, 28, a rotary driven gear key groove, 29 and a first flat key.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1 to 3, the liquid-based cell full-automatic staining machine based on the liquid-based thin-layer cell technology of the present invention mainly comprises: the device comprises a slide glass box 2, a slide glass 3, a plurality of staining boxes 4, a base 5, a switch 6 (standard part), a vertical shaft 8, a control display screen 9, a plurality of staining box bases 10, a first cylinder 13 (standard part), an upper cover 14, a second cylinder 16 (standard part), a waste liquid box, a lifting mechanism, a rotating mechanism and a control system.
The engine base 5 is of a square structure and is hollow inside, a through hole is formed in the center of the upper surface of the engine base 5, and an annular groove is formed in the upper surface of the engine base.
The upper cover 14 is a transparent square box, the inside of the upper cover is opened, the rear side of the upper cover 14 is movably connected with the rear side of the machine base 5, the lower ends of the first air cylinder 13 and the second air cylinder 16 are fixed on the rear side of the machine base 5 through bolts, and the upper ends of the first air cylinder 13 and the second air cylinder 16 are fixedly connected with the inner walls of the two sides of the upper cover 14. When the first air cylinder 13 and the second air cylinder 16 are started, the telescopic rods of the first air cylinder 13 and the second air cylinder 16 simultaneously extend upwards or retract downwards, and the upper cover 14 can be driven to open or close.
The plurality of dyeing box seats 10 are uniformly distributed in the annular groove on the upper surface of the machine base 5 by taking the center of the machine base 5 as a circle center and a circumference of 360 degrees.
The plurality of dyeing boxes 4 correspond to the plurality of dyeing box seats 10 one by one, and the dyeing boxes 4 are placed in the dyeing box seats 10. The dyeing box 4 is filled with dyeing liquid.
The lower part of the vertical shaft 8 is cylindrical, the upper part of the vertical shaft is cuboid, and the vertical shaft 8 is arranged in a central through hole of the base 5.
The glass slides 3 are arranged in the glass slide box 2, and a certain gap is arranged between two adjacent glass slides 3. The slide cassette 2 is fixed to the elevating mechanism. The lifting mechanism and the rotating mechanism are respectively connected with the vertical shaft 8, the rotating mechanism is arranged in the base 5, the lifting mechanism can move up and down on the vertical shaft 8, and the rotating mechanism can drive the vertical shaft 8 to rotate around the axis of the rotating mechanism.
The switch 6 and the control display screen 9 are both arranged on the front side surface of the machine base 5.
The waste liquid tank and the control system are installed inside the machine base 5. The waste liquid box is used for collecting waste liquid in the dyeing process.
The control system is respectively electrically connected with the switch 6, the control display screen 9, the lifting mechanism, the rotating mechanism, the first air cylinder 13 and the second air cylinder 16.
As shown in fig. 2, the rotating mechanism mainly includes: photoelectric encoder, first sensor 1, second sensor 7, thrust bearing 17, first ball bearing 18, rotatory driven gear 19, rotatory servo motor 20, rotatory driving gear 21, second ball bearing 22, identification system 23, bearing frame and motor cabinet.
The bearing seat and the motor seat are both fixed inside the machine base 5.
The thrust bearing 17, the first ball bearing 18, the rotary driven gear 19 and the second ball bearing 22 are sequentially arranged at the lower end of the vertical shaft 8 from bottom to top. The outer diameter of the lower end of the vertical shaft 8 is in transition fit with an inner hole of a thrust bearing 17, the lower end face of the thrust bearing 17 is in contact with a bearing seat in the base 5, the thrust bearing 17 mainly bears axial force, the upper end face of the thrust bearing 17 is in contact with the lower end face of an inner ring of a first ball bearing 18, the inner hole of the first ball bearing 18 and the inner hole of a second ball bearing 22 are in transition fit with the outer diameter of the vertical shaft 8, the inner ring of the first ball bearing 18 and the inner ring of the second ball bearing 22 both rotate together with the vertical shaft 8, and the outer diameter of the first ball bearing. The first ball bearing 18 and the second ball bearing 22 function to fix the vertical shaft 8 and reduce the rotational resistance.
The rotary driven gear 19 is arranged between the first ball bearing 18 and the second ball bearing 22, the inner diameter of the rotary driven gear 19 is in movable fit with the outer diameter of the vertical shaft 8, as shown in fig. 6 and 7, a vertical shaft key groove 27 is arranged on the outer diameter of the vertical shaft 8, a rotary driven gear key groove 28 is arranged on the inner hole of the rotary driven gear 19, first flat keys 29 are arranged on the vertical shaft key groove 27 and the rotary driven gear key groove 28, half of two side surfaces of each first flat key 29 are in transitional fit with the vertical shaft key groove 27, the other half of two side surfaces of each first flat key 29 are in movable fit with the side surface of the rotary driven gear key groove 28, torque is transmitted by the first flat keys 29, and the first flat keys 29 play a role in fixing the circumferential rotation.
The rotary servo motor 20 is fixed on a motor base inside the base 5 through bolts, and the rotary servo motor 20 is electrically connected with a control system. The rotary driven gear 19 is meshed with the rotary driving gear 21, an inner hole of the rotary driving gear 21 is tightly matched with an output shaft of the rotary servo motor 20, and the inner hole of the rotary driving gear 21 and the output shaft of the rotary servo motor 20 transmit torque through a second flat key. The connection of the inner bore of the rotary driving gear 21 to the output shaft of the rotary servomotor 20 is the same as the connection of the vertical shaft 8 to the rotary driven gear 19. Specifically, the method comprises the following steps: an output shaft key groove is formed in the outer diameter of an output shaft of the rotary servo motor 20, a rotary driving gear key groove is formed in an inner hole of the rotary driving gear 21, a second flat key is arranged on the output shaft key groove and the rotary driving gear key groove, one half of two side faces of the second flat key are in transition fit with the output shaft key groove, the other half of two side faces of the second flat key are in movable fit with the side face of the rotary driving gear key groove, torque is transmitted through the second flat key, and the second flat key plays a role in fixing the output shaft of the rotary servo motor 20 and.
When the rotary servo motor 20 rotates, the rotary driven gear 19 is driven to rotate together through the rotary driving gear 21, the rotary driving gear 21 and the rotary driven gear 19 are meshed to rotate, the vertical shaft 8 is driven to rotate, and meanwhile, the lifting mechanism on the vertical shaft 8 is driven to rotate together.
The first sensor 1, the recognition system 23 and the second sensor 7 are sequentially fixed on the vertical shaft 8 from top to bottom, and the first sensor 1, the recognition system 23 and the second sensor 7 are respectively electrically connected with the control system and used for detecting the start and stop of the corner position.
Role of the first sensor 1: when the slide glass box 2 reaches the position of the top edge, the dyeing is not performed, the first sensor 1 sends a signal to the control system, and the control system controls the rotating mechanism to rotate by an angle and simultaneously avoids collision.
The second sensor 7 functions: when the glass slide 3 is dyed, the second sensor 7 is used for detecting the distance between the glass slide 3 and the liquid level in the dyeing box 4 and transmitting a signal to the control system, and the control system controls the lifting mechanism to stay for a certain time, namely, the glass slide 3 is completely soaked in the dyeing liquid and is kept for a certain time.
Role of the recognition system 23: the identification system 23 can be realized by adopting the prior art in the invention, namely, the liquids in the staining boxes 4 are different, the boxes are externally provided with corresponding labels, namely corresponding two-dimensional codes, when the slide glass box 2 rotates, the identification system 23 can accurately capture the two-dimensional codes on the staining boxes 4, if the staining is needed, an instruction is sent to the control system, the control system controls the rotating mechanism to stop rotating, namely, the staining boxes 4 stop rotating, the lifting mechanism is started to stain, and meanwhile, the identification system 23 can identify the cell staining quality on the slide glass 3.
The photoelectric encoder is electrically connected with the control system. The photoelectric encoder is arranged on the vertical shaft 8 and is used for detecting the corner precision of the slide glass box 2 driven by the vertical shaft 8. If the rotation angle has an error, the photoelectric encoder sends a signal to the control system to correct the rotation of the servo motor 20.
As shown in fig. 1, 2, 3, 4 and 5, the lifting mechanism mainly includes: a lifting guide rail rack 11, a lifting servo motor 12, a slide support 15, a lifting drive gear 26, a slide lifting carriage 24 and a slide cassette fixing rod 25.
As shown in fig. 3, the lifting guide rack 11 is fixed on the outer wall of the upper end of the vertical shaft 8 through screws.
The lifting servo motor 12 is fixed on the slide lifting carriage 24 through screws, and the lifting servo motor 12 is electrically connected with the control system. Slide lifting carriage 24 is mounted on vertical shaft 8. Slide elevator carriage 24 is mounted internally on the upper end of vertical shaft 8 by an elevator drive gear 26. The output shaft of the lifting servo motor 12 is tightly matched with the lifting driving gear 26, the lifting driving gear 26 is meshed with the lifting guide rail rack 11, and the slide lifting carriage 24 is driven by the lifting servo motor 12 to slide up and down along the lifting guide rail rack 11.
When the output shaft of the elevation servo motor 12 rotates, the elevation driving gear 26 is driven to rotate together and is meshed with the elevation guide rail rack 11, and the slide glass elevation sliding frame 24, the slide glass bracket 15, the slide glass box fixing rod 25 and the slide glass box 2 are driven to move up and down or shake along the axial direction of the vertical shaft 8.
When the full-automatic liquid-based cell dyeing machine based on the liquid-based thin-layer cell technology is used, firstly, dyeing preparation work is carried out:
the switch 6 is turned on, the display screen 9 is controlled to display an operation page, the first air cylinder 13 and the second air cylinder 16 are started to act by controlling the display screen 9, the upper cover 14 is opened, the coloring agent is added into each coloring box 4, the lifting mechanism is started by controlling the display screen 9, the servo motor 12 is lifted to act, the glass slide box 2 is driven, the glass slides 3 are loaded, and about 6-10 glass slides 3 are loaded.
Then, dyeing work is carried out: the first air cylinder 13 and the second air cylinder 16 are started to act by controlling the display screen 9, and the upper cover 14 is closed.
The lifting servo motor 12 is started by controlling the display screen 9, the slide lifting carriage 24 is driven by the lifting servo motor 12 to slide downwards along the lifting guide rail rack 11, and meanwhile, the slide bracket 15, the slide box fixing rod 25 and the slide box 2 are driven to move downwards, the slide 3 can stay or shake in the corresponding dyeing box 4 according to requirements, and the stay time and the shaking amplitude are controllable; the slide lift carriage 24 is then driven by the lift servo motor 12 to slide upwardly along the lift rail rack 11, simultaneously, the slide glass bracket 15, the slide glass box fixing rod 25 and the slide glass box 2 are driven to move upwards, the rotary servo motor 20 is started to drive the rotary driven gear 19, the rotary driving gear 21 is meshed with the rotary driven gear 19 to rotate, the vertical shaft 8 is driven to rotate by a certain rotation angle, the rotation angle is controllable, the photoelectric encoder positions the rotation angle, at the moment, the glass slide box 2 corresponds to another dyeing box 4, the lifting servo motor 12 is started to drive the glass slide box 2 to move downwards, the glass slide 3 can stay or shake in the corresponding dyeing box 4, so that the dyeing is uniform and stable, the actions are repeated, the glass slide 3 is dyed in each dyeing groove 4, the automatic dyeing work of the glass slide 3 is realized, the whole dyeing process is completely automatically completed, and the machine program controls the dyeing process.
After dyeing is finished, the display screen 9 is controlled to start the first air cylinder 13 and the second air cylinder 16 to act, the upper cover 14 is opened, the lifting servo motor 12 is started by controlling the display screen 9 to drive the slide glass box 2 to move upwards, the slide glass box fixing rod 25 is lifted, the slide glass box 2 is taken down, each slide glass 3 is taken down to be packaged, and dyeing work is finished.
In the description of the present invention, it is to be understood that the terms "central", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be considered as limiting the scope of the present invention.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: it is to be understood that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof, but such modifications or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. Full-automatic dyeing machine of liquid-based cell based on liquid-based thin layer cell technique, its characterized in that includes:
a machine base;
an upper cover movably connected with one side of the base;
the first cylinder and the second cylinder are fixed on one side of the base and are fixed with the inner walls of the two sides of the upper cover;
a plurality of dyeing box seats are uniformly distributed on the upper surface of the machine seat by taking the center of the machine seat as a circle center;
a staining box placed in the staining box seat;
a dyeing liquid placed in the dyeing box;
a vertical shaft installed in the center through hole of the machine base;
the rotating mechanism is arranged in the base and is connected with the lower end of the vertical shaft, and the rotating mechanism can drive the vertical shaft to rotate around the axis of the rotating mechanism;
the lifting mechanism is slidably mounted on the vertical shaft and can move up and down along the vertical shaft;
a slide cassette secured to the lift mechanism, the slide cassette having a slide disposed therein.
2. The full-automatic liquid-based cell dyeing machine based on the liquid-based thin-layer cell technology as claimed in claim 1, further comprising a switch and a control display screen mounted on the outer side of the machine base, and a control system mounted inside the machine base, wherein the control system is electrically connected with the switch, the control display screen, the lifting mechanism, the rotating mechanism, the first cylinder and the second cylinder respectively.
3. The liquid-based thin layer cell technology-based liquid-based cell full-automatic staining machine of claim 2, wherein the rotating mechanism comprises:
the bearing seat and the motor seat are fixed in the base;
the lower end of the vertical shaft is in transition fit with an inner hole of the thrust bearing, the lower end face of the thrust bearing is in contact with the bearing seat, the upper end face of the thrust bearing is in contact with the lower end face of an inner ring of the first ball bearing, the inner hole of the first ball bearing and the inner hole of the second ball bearing are in transition fit with the outer diameter of the vertical shaft, the inner ring of the first ball bearing and the inner ring of the second ball bearing rotate together with the vertical shaft, the outer diameter of the first ball bearing and the outer diameter of the second ball bearing are fixedly connected with the bearing seat, and the inner diameter of the rotary driven gear is in movable fit with the outer diameter;
the rotary servo motor is fixed on the motor base and is electrically connected with the control system;
the inner hole of the rotary driving gear is tightly matched with an output shaft of the rotary servo motor;
the first sensor, the identification system and the second sensor are sequentially fixed on the vertical shaft from top to bottom; the first sensor, the identification system and the second sensor are electrically connected with the control system respectively.
4. The full-automatic liquid-based cell dyeing machine based on the liquid-based thin-layer cell technology as claimed in claim 3, wherein a vertical shaft key groove is formed on the outer diameter of the vertical shaft, a rotating driven gear key groove is formed on the inner hole of the rotating driven gear, a first flat key is installed on the vertical shaft key groove and the rotating driven gear key groove, half of the two side surfaces of the first flat key is in transition fit with the vertical shaft key groove, and the other half of the two side surfaces of the first flat key is in movable fit with the side surfaces of the rotating driven gear key groove.
5. The full-automatic liquid-based cell dyeing machine based on the liquid-based thin-layer cell technology as claimed in claim 3, wherein the outer diameter of the output shaft of the rotary servo motor is provided with an output shaft key groove, the inner hole of the rotary driving gear is provided with a rotary driving gear key groove, a second flat key is arranged on the output shaft key groove and the rotary driving gear key groove, one half of two side surfaces of the second flat key is in transition fit with the output shaft key groove, the other half of two side surfaces of the second flat key is in movable fit with the side surfaces of the rotary driving gear key groove, torque is transmitted by the second flat key, and the second flat key plays a role in fixing the output shaft of the rotary servo.
6. The full-automatic liquid-based cell staining machine based on liquid-based thin-layer cell technology of claim 3, wherein the rotating mechanism further comprises: a photoelectric encoder mounted on the vertical shaft; the photoelectric encoder is electrically connected with the control system.
7. The full-automatic liquid-based cell staining machine based on liquid-based thin-layer cell technology of claim 2, wherein the lifting mechanism comprises:
a lifting guide rail rack fixed on the outer wall of the upper end of the vertical shaft;
a slide glass lifting sliding frame is arranged at the upper end of the vertical shaft through a lifting driving gear;
the lifting servo motor is fixed on the slide glass lifting sliding frame and is electrically connected with the control system, an output shaft of the lifting servo motor is tightly matched with the lifting driving gear, and the lifting driving gear is meshed with the lifting guide rail rack;
a slide support fixed to the slide lifting carriage;
the slide glass box fixing rod is vertically fixed at the other end of the slide glass bracket, a dovetail groove is formed in the other end of the slide glass box fixing rod, a dovetail structure is arranged at the upper end of the slide glass box, and the slide glass box is clamped and fixed in the dovetail groove of the slide glass box fixing rod through the dovetail structure; the slide glass box is driven to move up and down or shake by the lifting servo motor.
8. The full-automatic liquid-based cell staining machine based on the liquid-based thin-layer cell technology of claim 1, further comprising a waste liquid tank installed inside the machine base.
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| CN202011240556.XA CN112229698A (en) | 2020-11-09 | 2020-11-09 | Liquid-based thin-layer cell technology-based liquid-based cell full-automatic dyeing machine |
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| CN202011240556.XA CN112229698A (en) | 2020-11-09 | 2020-11-09 | Liquid-based thin-layer cell technology-based liquid-based cell full-automatic dyeing machine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113959815A (en) * | 2021-11-08 | 2022-01-21 | 重庆医药高等专科学校 | Cell staining device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113959815A (en) * | 2021-11-08 | 2022-01-21 | 重庆医药高等专科学校 | Cell staining device |
| CN113959815B (en) * | 2021-11-08 | 2023-08-08 | 重庆医药高等专科学校 | Cell staining device |
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